Frontiers in Microbiology最新文献

{"title":"Direct and indirect effects of land use on microbiomes of trap-nesting solitary bee larvae and nests.","authors":"Birte Peters, Sara Diana Leonhardt, Michael Schloter, Alexander Keller","doi":"10.3389/fmicb.2024.1513096","DOIUrl":"10.3389/fmicb.2024.1513096","url":null,"abstract":"<p><strong>Introduction: </strong>The global decline in biodiversity and insect populations highlights the urgent need to conserve ecosystem functions, such as plant pollination by solitary bees. Human activities, particularly agricultural intensification, pose significant threats to these essential services. Changes in land use alter resource and nest site availability, pesticide exposure and other factors impacting the richness, diversity, and health of solitary bee species. In this study, we investigated yet another facet currently less well investigated in such context: Microbial communities associated with wild bees play crucial roles in larval development, metabolism, immunity and overall bee health. However, the drivers and dynamics of healthy microbiome in solitary bees are still poorly understood, especially regarding the direct and indirect effects of land use on the diversity and composition of these microbial communities.</p><p><strong>Methods: </strong>We examined bacterial communities in the offspring and nest materials of the Megachilid trap-nesting solitary bee, Osmia bicornis, along a gradient of land use intensification by 16S rRNA gene metabarcoding. Given that landscape composition, climatic conditions, and food resources are known to influence microbial compositions in solitary bee species, we hypothesized that land use changes would alter resources available for food and nest material collection and thereby affecting the microbiomes in offspring and their nest environments. We anticipated reduced microbial diversity and altered composition with increased land use intensification, which is known to decrease the number and diversity of resources, including the pool of floral and soil bacteria in the surrounding environment.</p><p><strong>Results: </strong>As expected, we observed significant shifts in the bacterial composition and diversity of bees and their nests across varying degrees of land use intensity, differing in management types and the availability of flowers. The Shannon diversity of bacteria in nest materials (larval pollen provision, soil nest enclosure) and larval guts decreased with increasing land use intensity. However, the pupae microbiome remained unaffected, indicating a reorganization of the microbiome during metamorphosis, which is not significantly influenced by land use and available resources.</p><p><strong>Discussion: </strong>Our findings provide new insights into the factors shaping environmental transmission and changes in solitary bee microbiomes. This understanding is crucial for comprehending the impacts of intensive land use on wild bee health and developing strategies to mitigate these effects.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"15 ","pages":"1513096"},"PeriodicalIF":4.0,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11753253/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143022654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Shotgun metagenomics reveals the interplay between microbiome diversity and environmental gradients in the first marine protected area in the northern Arabian Gulf.","authors":"Saja A Fakhraldeen, Rakhesh Madhusoodhanan, Nazima Habibi, Sakinah Al-Haddad, Surendraraj Alagarsamy, Sabeena F K Habeebullah, Walid M Al-Zakri, Fathima Thuslim, Loreta Fernandes, Faiza Al-Yamani, Turki Al-Said","doi":"10.3389/fmicb.2024.1479542","DOIUrl":"10.3389/fmicb.2024.1479542","url":null,"abstract":"<p><strong>Introduction: </strong>The northwest Arabian Gulf encounters significant anthropogenic pressures, including nutrient enrichment from coastal development and effluent discharge.</p><p><strong>Methods: </strong>This study presents the first shotgun metagenomics-based characterization of microbial communities in Kuwaiti waters of the northwest Arabian Gulf, focusing on Kuwait's first Marine Protected Area (MPA) in Sulaibikhat Bay, a vital nursery ground for commercially important fish.</p><p><strong>Results: </strong>Analysis revealed significantly higher microbial diversity within the MPA compared to adjacent waters, with Rhodobacteraceae (27.8%) and Flavobacteriaceae (15.3%) being dominant. Elevated inorganic phosphorus, nitrogen, and salinity were key factors driving this diversity. Multivariate analysis highlighted phosphate as a critical component affecting the MPA microbial community structure, particularly for the families Microbacteriaceae, Flavobacteriaceae, and Rhodobacteraceae.</p><p><strong>Discussion: </strong>This study underscores the ecological importance of MPAs and highlights the impact of nutrient enrichment and other environmental stressors on microbial diversity, emphasizing the need to reduce nutrient influx to mitigate eutrophication and enhance marine ecosystem resilience in stressed environments.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"15 ","pages":"1479542"},"PeriodicalIF":4.0,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11755137/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143028410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum: Ribosomal protein L40e fused with a ubiquitin moiety is essential for the vegetative growth, morphological homeostasis, cell cycle progression, and pathogenicity of <i>Cryptococcus neoformans</i>.","authors":"Jingyu Zhao, Yali Yang, Yibin Fan, Jiu Yi, Chao Zhang, Zhongkai Gu, Weihua Pan, Julin Gu, Wanqing Liao, Wei Fang","doi":"10.3389/fmicb.2024.1545744","DOIUrl":"https://doi.org/10.3389/fmicb.2024.1545744","url":null,"abstract":"<p><p>[This corrects the article DOI: 10.3389/fmicb.2020.570269.].</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"15 ","pages":"1545744"},"PeriodicalIF":4.0,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11754878/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143028173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pyrrocidines A and B demonstrate synergistic inhibition of <i>Fusarium verticillioides</i> growth.","authors":"Lily W Lofton, Quentin D Read, Hailey L Hamilton, Anthony E Glenn, Jaci A Hawkins, Trevor R Mitchell, Scott E Gold","doi":"10.3389/fmicb.2024.1480920","DOIUrl":"10.3389/fmicb.2024.1480920","url":null,"abstract":"<p><p><i>Fusarium verticillioides</i>-a mycotoxigenic fungus and food safety threat-coinhabits maize kernels with <i>Sarocladium zeae</i>. This protective endophyte produces secondary metabolites of interest, pyrrocidines A and B, which inhibit the growth of <i>F. verticillioides</i> and specifically block fumonisin biosynthesis. Previous transcriptomic analyses found <i>FvZBD1</i> (FVEG_00314), a gene adjacent to the fumonisin biosynthetic gene cluster, to be induced over 4,000-fold in response to pyrrocidine challenge. Deletion of <i>FvZBD1</i> resulted in dramatic increases in fumonisin production (FB₁ >30-fold). Here, using pyrrocidine dose-response assays, we discovered a potent synergy between pyrrocidines A and B, where they functioned powerfully together to inhibit <i>F. verticillioides</i> growth. Further, results provided evidence that <i>FvZBD1</i> confers partial tolerance to pyrrocidines, particularly pyrrocidine A, and that pyrrocidine functions through <i>FvZBD1</i> to effectively eliminate fumonisin biosynthesis. Additionally, we showed that the <i>FvABC3</i> (FVEG_11089) mutant, earlier described as hypersensitive to pyrrocidine, is particularly sensitive to pyrrocidine B. Thus, pyrrocidine A and B show different target specificity (<i>FvZBD1</i> or <i>FvABC3</i>) and synergistic action. These findings will help inform the optimization of maximally efficacious <i>S. zeae</i> strains for eliminating <i>F. verticillioides</i> colonization and fumonisin contamination in maize cropping systems. This novel study contributes significantly to our knowledge of competitive microorganism relationships and the role of secondary metabolites in antagonistic fungal-fungal interactions.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"15 ","pages":"1480920"},"PeriodicalIF":4.0,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11754276/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143028398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Editorial: Women in microbe and virus interactions with plants: 2022/2023.","authors":"Sonia Chadha, Esther Menendez, Nuria Montes","doi":"10.3389/fmicb.2024.1532112","DOIUrl":"10.3389/fmicb.2024.1532112","url":null,"abstract":"","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"15 ","pages":"1532112"},"PeriodicalIF":4.0,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11754181/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143028221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel silver nanoparticle-based biomaterials for combating <i>Klebsiella pneumoniae</i> biofilms.","authors":"Eslam Elashkar, Rihaf Alfaraj, Ola M El-Borady, Mahmoud M Amer, Abdelazeem M Algammal, Azza S El-Demerdash","doi":"10.3389/fmicb.2024.1507274","DOIUrl":"10.3389/fmicb.2024.1507274","url":null,"abstract":"<p><strong>Background: </strong><i>Klebsiella pneumoniae</i> is a significant nosocomial pathogen that has developed resistance to multiple antibiotics, often forming biofilms that enhance its virulence. This study investigated the efficacy of a novel nanoformulation, AgNPs@chitosan-NaF, in combating <i>K. pneumoniae</i> biofilms.</p><p><strong>Methods: </strong>Antimicrobial susceptibility testing was performed to assess the antibiotic resistance profile of <i>K. pneumoniae</i> isolates. The antibiofilm activity of AgNPs@chitosan-NaF was evaluated using crystal violet staining and scanning electron microscopy. The underlying mechanisms of action were investigated through gene expression analysis.</p><p><strong>Results: </strong>The majority of <i>K. pneumoniae</i> isolates exhibited high levels of multidrug resistance. AgNPs@chitosan-NaF demonstrated superior biofilm inhibition compared to AgNPs@chitosan, significantly reducing biofilm biomass and disrupting biofilm architecture at MICs ranging from 0.125 to 1 μg/mL. Mechanistic studies revealed that the nanoformulation downregulated the expression of key biofilm-associated genes, including <i>treC</i>, <i>fimA</i>, <i>mrkA</i>, and <i>ecpA</i>. While AgNPs@chitosan-NaF exhibited a concentration-dependent cytotoxic effect on both normal and cancer cell lines, minimal cytotoxicity was observed at concentrations below 31.25 μg/mL.</p><p><strong>Conclusion: </strong>This study highlights the synergistic effect of silver nanoparticles, chitosan, and sodium fluoride in combating <i>K. pneumoniae</i> biofilms. The nanoformulation, AgNPs@chitosan-NaF, emerges as a promising therapeutic strategy to address the challenge of multidrug-resistant bacterial infections.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"15 ","pages":"1507274"},"PeriodicalIF":4.0,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11754292/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143028290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence and characterization of IncQ1α-mediated multi-drug resistance in <i>Proteus mirabilis</i> Isolated from pigs in Kunming, Yunnan, China.","authors":"Hongmei Liu, Na Xia, Fanan Suksawat, Bundit Tengjaroenkul, Yue Hu, Xiaofeng Zhou, Xiaojiang Li, Cuiqin Huang, Yinli Bao, Qiong Wu, Chunrong Zhang, Sunpetch Angkititrakul, Bin Xiang, Xin Wu","doi":"10.3389/fmicb.2024.1483633","DOIUrl":"10.3389/fmicb.2024.1483633","url":null,"abstract":"<p><strong>Background: </strong><i>Proteus mirabilis</i> is a conditionally pathogenic bacterium that is inherently resistant to polymyxin and tigecycline, largely due to antibiotic resistance genes (ARGs). These ARGs can be horizontally transferred to other bacteria, raising concerns about the Inc plasmid-mediated ARG transmission from <i>Proteus mirabilis</i>, which poses a serious public health threat. This study aims to investigate the presence of Inc plasmid types in pig-derived <i>Proteus mirabilis</i> in Kunming, Yunnan, China.</p><p><strong>Methods: </strong>Fecal samples were collected from pig farms across six districts of Kunming (Luquan, Jinning, Yiliang, Anning, Songming, and Xundian) from 2022 to 2023. <i>Proteus mirabilis</i> isolates were identified using <i>IDS</i> and <i>16S rRNA</i> gene sequencing. Then, positive strains underwent antimicrobial susceptibility testing and incompatibility plasmid typing. Multi-drug-resistant isolates with positive incompatibility plasmid genes were selected for whole-genome sequencing. Resistance and Inc group data were then isolated and compared with 126 complete genome sequences from public databases. Whole-genome multi-locus sequence typing, resistance group analysis, genomic island prediction, and plasmid structural gene analysis were performed.</p><p><strong>Results: </strong>A total of 30 isolates were obtained from 230 samples, yielding a prevalence of 13.04%. All isolates exhibited multi-drug resistance, with 100% resistance to cotrimoxazole, erythromycin, penicillin G, chloramphenicol, ampicillin, and streptomycin. Among these, 15 isolates tested positive for the IncQ1α plasmid <i>repC</i> gene. The two most multi-drug-resistant and <i>repC</i>-positive strains, NO. 15 and 21, were sequenced to compare genomic features on Inc groups and ARGs with public data. Genome analysis revealed that the <i>repC</i> gene was primarily associated with IncQ1α, with structural genes from other F-type plasmids (<i>TraV</i>, <i>TraU</i>, <i>TraN</i>, <i>TraL</i>, <i>TraK</i>, <i>TraI</i>, <i>TraH</i>, <i>TraG</i>, <i>TraF</i>, <i>TraE</i>/<i>GumN</i>, and <i>TraA</i>) also present. Strain NO. 15 carried 33 ARGs, and strain NO. 21 carried 38 ARGs, conferring resistance to tetracyclines, fluoroquinolones, aminoglycosides, sulfonamides, peptides, chloramphenicol, cephalosporins, lincomycins, macrolides, and 2-aminopyrimidines.</p><p><strong>Conclusion: </strong>The <i>repC</i> gene is primarily associated with IncQ1α, with structural genes from other F-type plasmids. A comparison with 126 public genome datasets confirmed this association.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"15 ","pages":"1483633"},"PeriodicalIF":4.0,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11754265/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143028379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"16S rRNA sequencing-based evaluation of the protective effects of key gut microbiota on inhaled allergen-induced allergic rhinitis.","authors":"Yi Tang, Yongchuan She, Danping Chen, Yibo Zhou, Dan Xie, Zhai Liu","doi":"10.3389/fmicb.2024.1497262","DOIUrl":"10.3389/fmicb.2024.1497262","url":null,"abstract":"<p><strong>Introduction: </strong>Allergic rhinitis (AR) is a common respiratory disorder influenced by various factors in its pathogenesis. Recent studies have begun to emphasize the significant role of gut microbiota in immune modulation and its potential association with the development of AR. This research aims to characterize the gut microbiota of patients with AR who are sensitized via inhalation, utilizing 16S rRNA sequencing to shed light on the pathogenesis of AR and identify potential therapeutic targets.</p><p><strong>Methods: </strong>To achieve the study's objectives, we compared the microbiota profiles between patients with AR and healthy controls. Microbial diversity was assessed using alpha and beta diversity indices, and differential microbiota populations were identified through Linear discriminant analysis Effect Size (LEfSe) analysis. A Least Absolute Shrinkage and Selection Operator (LASSO) regression model was employed to pinpoint key species. Additionally, PICRUSt2 was utilized to predict the functional pathways associated with these identified species.</p><p><strong>Results: </strong>The analysis identified a total of 1,122 common species, along with 1,803 species associated with AR and 1,739 species associated with healthy controls. LEfSe analysis revealed 20 significant discrepancies at the genus level. The LASSO regression model identified 8 key genera, including <i>Prevotellaceae</i> UCG-004 and <i>Rhodococcus</i>, which exhibited AUC values exceeding 0.7, indicating strong diagnostic potential. Furthermore, functional pathway analysis suggested that these pivotal species are involved in pathways such as L-lysine biosynthesis and photorespiration, potentially contributing to the pathogenesis of AR.</p><p><strong>Discussion: </strong>This study identifies critical gut microbiota that could serve as potential biomarkers for allergic rhinitis, providing new insights into its pathogenesis and offering avenues for future therapeutic strategies. Further investigation into these microbiota may lead to enhanced understanding and management of AR.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"15 ","pages":"1497262"},"PeriodicalIF":4.0,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11756352/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143028616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influential articles in autism and gut microbiota: bibliometric profile and research trends.","authors":"Jiangbo Ying, Melvyn Weibin Zhang, Ker-Chiah Wei, Sunny H Wong, Mythily Subramaniam","doi":"10.3389/fmicb.2024.1401597","DOIUrl":"10.3389/fmicb.2024.1401597","url":null,"abstract":"<p><strong>Objective: </strong>Autism spectrum disorder (ASD) is a common neurodevelopmental disorder. Increasing evidence suggests that it is potentially related to gut microbiota, but no prior bibliometric analysis has been performed to explore the most influential works in the relationships between ASD and gut microbiota. In this study, we conducted an in-depth analysis of the most-cited articles in this field, aiming to provide insights to the existing body of research and guide future directions.</p><p><strong>Methods: </strong>A search strategy was constructed and conducted in the Web of Science database to identify the 100 most-cited papers in ASD and gut microbiota. The Biblioshiny package in R was used to analyze and visualize the relevant information, including citation counts, country distributions, authors, journals, and thematic analysis. Correlation and comparison analyses were performed using SPSS software.</p><p><strong>Results: </strong>The top 100 influential manuscripts were published between 2000 and 2021, with a total citation of 40,662. The average number of citations annually increased over the years and was significantly correlated to the year of publication (<i>r</i> = 0.481, <i>p</i> < 0.01, Spearman's rho test). The United States was involved in the highest number of publications (<i>n</i> = 42). The number of publications in the journal was not significantly related to the journal's latest impact factor (<i>r</i> = 0.016, <i>p</i> > 0.05, Spearman's rho test). Co-occurrence network and thematic analysis identified several important areas, such as microbial metabolites of short-chain fatty acids and overlaps with irritable bowel syndrome.</p><p><strong>Conclusion: </strong>This bibliometric analysis provides the key information of the most influential studies in the area of ASD and gut microbiota, and suggests the hot topics and future directions. The findings of this study can serve as a valuable reference for researchers and policymakers, guiding the development and implementation of the scientific research strategies in this area.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"15 ","pages":"1401597"},"PeriodicalIF":4.0,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11755156/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143028246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Long-term adoption of plow tillage and green manure improves soil physicochemical properties and optimizes microbial communities under a continuous peanut monoculture system.","authors":"Yunfeng Yao, Rongyv Zhu, Xiangdong Li, Guoqing Hu, Yuanjie Dong, Zhaoxin Liu","doi":"10.3389/fmicb.2024.1513528","DOIUrl":"10.3389/fmicb.2024.1513528","url":null,"abstract":"<p><p>Continuous monocropping of peanuts (<i>Arachis hypogaea L</i>.) often results in yield decline and soil degradation. The combination of green manure (GM) with tillage practices has been proposed as a sustainable strategy to maintain high crop productivity and improve soil quality. This study investigates the long-term effects of 8 years of GM application combined with plow tillage on soil microbial communities and physicochemical properties under a peanut monocropping system. Treatments included: (i) no tillage (NT); (ii) plow tillage before the winter fallow period (PT); and (iii) growing ryegrass (<i>Lolium perenne L</i>.) during the winter period and applying it as GM before planting next-stubble peanut (PTGM). It was found that both PTGM and PT remarkably decreased the average bulk density (BD), while elevated the mean soil porosity (SP) in 0-30 cm soil layer. Moreover, PTGM significantly increased available potassium (AK), available phosphorus (AP), total nitrogen (TN), and soil organic matter (SOM). Peanut pod yields in the PTGM treatment were 14.1 and 7.2% higher compared to the PT and NT treatments, respectively. Additionally, PTGM could promote shifts in soil bacteria compositions, increasing the abundance of Actinobacteria and Firmicutes while reducing that of Chloroflexi. For fungal abundances, PTGM elevated the abundances of Ascomycota and Basidiomycote. Redundancy analysis demonstrated that SOM, TN, AK, and AP were positively related to dominant flora of fungi and bacteria in PTGM, while negatively related to dominant flora of fungi and bacteria in NT. Overall, tillage practices have the potential to reshape the microbial community during the peanut growing season, primarily due to the influence of SOM, TN, and AP content in shaping microbial diversity and composition. Our study highlights that plow tillage combined with GM application may serve as an effective tillage practice in the future to mitigate continuous cropping obstacles by modulating soil microbial communities, improving soil nutrients and fertility, and enhancing crop productivity.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"15 ","pages":"1513528"},"PeriodicalIF":4.0,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11754393/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143028253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}