Frontiers in Microbiology最新文献

{"title":"Isolation, characterization, and genomic analysis of a novel bacteriophage vB_Kp_XP4 targeting hypervirulent and multidrug-resistant <i>Klebsiella pneumoniae</i>.","authors":"Xiaocui Peng, Jianliang Chang, Hongxia Zhang, Xiaoyu Li, Changhong Zhang, Shiyan Jiao, Chengxiu Lv, Na Wang, Jun Zhao, Bu Wang, Wei Zhang, Zhihua Zhang","doi":"10.3389/fmicb.2025.1491961","DOIUrl":"10.3389/fmicb.2025.1491961","url":null,"abstract":"<p><strong>Introduction: </strong>Hypervirulent and multidrug-resistant <i>Klebsiella pneumoniae</i> (hvKP and MDR-KP) are significant public health threats. This study aimed to isolate a lytic bacteriophage targeting these high-risk strains, systematically characterize its biological properties, genomic features, and therapeutic efficacy, and establish a foundation for clinical phage therapy and novel antimicrobial development.</p><p><strong>Methods: </strong>The phage vB_Kp_XP4 was isolated from river water using the double-layer agar plate method with the clinically isolated strain P4 as the host. Morphology was analyzed via transmission electron microscopy (TEM). Host range, pH, and thermal stability were assessed using spot assays and OD₆₃₀ measurements. One-step growth curves determined the latent period and burst size. Whole-genome sequencing and phylogenetic analysis were performed. Therapeutic efficacy and safety were evaluated in a <i>Galleria mellonella</i> infection model.</p><p><strong>Results: </strong>TEM revealed Phage vB_Kp_XP4 as a tailed phage with an icosahedral head and a long, flexible tail. It lysed an hvKP strain (carrying <i>rmp</i>, <i>peg</i>, <i>iuc</i>, <i>iro</i> genes) and an MDR-KP strain (resistant to carbapenems, fluoroquinolones, etc.), with an optimal MOI of 0.1 and latent period <10 minutes. Stability was maintained at pH 4-11 and ≤70°C. Whole-genome sequencing revealed a linear double-stranded DNA genome of 44,344 bp with a G+C content of 53.80%. The genome comprised 54 coding sequences and lacked lysogenic, virulence, or antibiotic resistance genes. Phylogenetic analysis positioned phage vB_Kp_XP4 as a novel species within the genus <i>Drulisvirus</i>, family <i>Autographiviridae</i>. In the <i>Galleria mellonella</i> model, vB_Kp_XP4 prolonged survival of P4-infected larvae (<i>P</i> < 0.001).</p><p><strong>Conclusion: </strong>Phage vB_Kp_XP4 exhibits high stability, specificity, potent lytic activity, and no undesirable genes, demonstrating effective in vivo therapeutic efficacy, suggest its potential for clinical applications against <i>Klebsiella pneumoniae</i> infections. The presence of multiple halos during plaque formation further enhances its research value. The complete genome sequence has been submitted to GenBank under accession number PP663283.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1491961"},"PeriodicalIF":4.0,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11925924/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143691186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differentiation and response mechanisms of the endophytic flora of plants ecologically restored in the ilmenite area.","authors":"Xin Yu, Junqiang Xu, Ziping Zou, Yunfeng Zhang, Peng Wu, Qiang Li","doi":"10.3389/fmicb.2025.1555309","DOIUrl":"10.3389/fmicb.2025.1555309","url":null,"abstract":"<p><p>Heavy metal contamination in soil is a serious environmental challenge, and abandoned mining areas are of particular concern. In order to rehabilitate the ecology of these areas. In this study, we used ICP-MS and potentiometric method to analyze the soil physicochemical and then endophytic bacteria of remediation plants with the help of 16sRNA sequencing, in order to investigate the ecological remediation of abandoned ilmenite mine and the effect of soil microbiology by seven common plants. The results revealed that the abandonment of ilmenite significantly increased the contents of total phosphorus, total potassium, available potassium, iron, and lead in the surrounding soils. It also affected the richness and diversity of endophytic bacterial communities. Pvi had the highest richness, while Tsi had the lowest richness (<i>P</i> < 0.05). A total of 28 phyla, 69 classes, 171 orders, and 521 genera were identified. A total of nine core OTUs were found: <i>Stenotrophomonas</i>, <i>Chryseobacterium</i>, <i>Lactobacillus</i>, <i>Clostridium_sensu_stricto_12</i>, <i>Prevotella</i>, <i>Lactobacillus</i>, <i>Bradyrhizobium</i>, <i>Nocardioides</i>, and <i>Delftia</i>. Beta diversity analysis revealed that the community structure of the endophytic bacteria differed during the remediation process at the ilmenite site. Functional prediction revealed upregulation of Dco transporter protein function, DNA-binding transcriptional regulators, glyoxalase or related metal-dependent hydrolases, acyl coenzyme A synthetases, ATPase components, amino acid synthesis, and cellular respiration-related functions. Pearson correlation analysis revealed that the SOC, TK, AN, AK, and Zn contents were significantly correlated with α diversity. Redundancy analysis (RDA) revealed that Actinobacteriota was significantly positively correlated with soil SOD, AN, TN, and TK contents. For the first time, this study revealed the interactions among plants, endophytic bacteria and soil pollutants, laying a theoretical basis for screening specific plant endophytic bacteria for ecological restoration.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1555309"},"PeriodicalIF":4.0,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11926159/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143691578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tripartite microbial augmentation of <i>Bradyrhizobium diazoefficiens</i>, <i>Bacillus</i> sp. MN54, and <i>Piriformospora indica</i> on growth, yield, and nutrient profiling of soybean (<i>Glycine max</i> L.).","authors":"Munazza Rafique, Muhammad Naveed, Muhammad Zahid Mumtaz, Abid Niaz, Saud Alamri, Sajid Ur Rehman, Manzer H Siddiqui, Adnan Mustafa","doi":"10.3389/fmicb.2024.1437489","DOIUrl":"10.3389/fmicb.2024.1437489","url":null,"abstract":"<p><strong>Introduction: </strong>Enhancing productivity and nutrient content of soybean (<i>Glycine max</i> L.) is vital for sustainable agriculture. The utilization of beneficial bacterial and fungal strains has shown promising results in promoting plant growth and improving nutrient uptake. However, the effects of the individual and interactions of such microbes on soybean growth, yield, and nutrient profiling remain inadequately understood. Thus, there is a pressing need to comprehensively investigate the impact of tripartite microbial augmentation on soybean cultivation.</p><p><strong>Methods: </strong>This field study aims to elucidate the synergistic mechanisms underlying the interactions between <i>Bacillus</i> sp. MN54, <i>Bradyrhizobium diazoefficiens</i>, and <i>Piriformospora indica</i> and their collective influence on soybean growth parameters, yield and nutritional quality.</p><p><strong>Results: </strong><i>In vivo</i> compatibility tests revealed that consortium applications led to a maximum of 90% soybean germination. The field study demonstrated a significant increase in plant height (17.01%), nodules plant^-1 (17.35%), pods plant^-1 (12.11%), and grain yield (20.50%) due to triple inoculation over untreated control. The triple inoculation also significantly increased chlorophyll a, b, and leghemoglobin contents by 19.38, 21.01, and 14.28%, respectively, compared to control. Triple inoculation promoted crude fiber, protein, and oil content by 14.92, 8.78, and 10.52%, respectively, compared to the untreated control. The increase in nitrogen content by 7.33% in grains and 6.26% in stover and phosphorus by 11.31% in grains and 12.72% in stover was observed through triple application over untreated control.</p><p><strong>Discussion: </strong>Our findings highlight the potential of microbial inoculants as biofertilizers in sustainable soybean production. The triple inoculation with <i>Bacillus</i> sp. MN54, <i>Bradyrhizobium diazoefficiens</i>, and <i>Piriformospora indica</i> significantly improved soybean growth, yield, grain quality attributes, and nutrient uptake. This microbial consortium application could help to enhance agricultural productivity by boosting the nodulation in soybean and improving synergism between the microbial strains.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"15 ","pages":"1437489"},"PeriodicalIF":4.0,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11925901/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143691576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unraveling the pathogenomics of <i>Rhizoctonia solani</i> infecting proso millet (<i>Panicum miliaceum</i> L.): genomic perspective on ruthless virulence and adaptive evolution.","authors":"Prasanna S Koti, T S S K Patro, K B Palanna, B Jeevan, Porapu Prasanth, G V Ramesh, N Anuradha, Y Sandhya Rani, Ungata Triveni, K Lavanya Devi, T Tharana Poonacha, Farooq Khan, Boda Praveen, M Divya, D Sabina Mary, V Prasanna Kumari, T E Nagaraja, R Madhusudhana, C Tara Satyavathi","doi":"10.3389/fmicb.2025.1557991","DOIUrl":"10.3389/fmicb.2025.1557991","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Introduction: &lt;/strong&gt;Banded sheath blight (Bsb), caused by &lt;i&gt;Rhizoctonia solani&lt;/i&gt;, is an emerging threat to proso millet cultivation, significantly impacting yield and grain quality. This study on the pathogenomics of &lt;i&gt;R. solani&lt;/i&gt; seeks to unravel its genetic mechanisms, identify key virulence factors, decode host-pathogen interactions, and pinpoint molecular targets for effective control strategies.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;&lt;i&gt;R. solani&lt;/i&gt; isolates were collected from various regions across India, resulting in six distinct isolates. These isolates were comprehensively characterized through morphological observations, molecular analyses, and virulence assessments to gain comprehensive insights into their diversity and pathogenic potential. The most virulent strain, designated VAP-1, infecting proso millet, was sequenced using the Illumina platform and &lt;i&gt;de novo&lt;/i&gt; assembled using the SPAdes assembler, resulting in a highly complete genome. Functional regions of the genome were predicted and annotated using Funannotate. A subsequent comparative genomics study and secretome analysis were conducted to support functional genomic investigations.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;The VAP-1 genome assembly resulted in a total size of 47.12 Mb, with approximately 17.62% of the genome consisting of repetitive sequences, predominantly dominated by interspersed elements (around 97.8%). These interspersed elements were primarily classified as retrotransposons (72%), with DNA transposons comprising a smaller proportion (5%), while the remaining interspersed sequences were not fully annotated. Functional analysis of the genome revealed significant enrichment in KEGG pathways, including \"Carbohydrate metabolism,\" \"Translation,\" \"Signal transduction,\" and \"Transport and catabolism.\" In addition, Gene Ontology (GO) terms such as \"Proteolysis,\" \"Membrane,\" and \"ATP binding\" were notably enriched. The secretory protein profile of the VAP-1 genome from &lt;i&gt;R. solani&lt;/i&gt; features key proteins from the major facilitator superfamily (MFS) transporters, (Trans) glycosidases, P-loop containing nucleoside triphosphate hydrolases, and galactose oxidase, all within the central domain superfamily. Glycoside hydrolases represent the largest class of CAZymes in the VAP-1 genome. Comparative genomic analysis of VAP-1 with other &lt;i&gt;R. solani&lt;/i&gt; strains infecting Poaceae (e.g., rice) and non-Poaceae (e.g., sugar beet and tobacco) hosts showed that VAP-1 clusters closely with rice-infecting strains at the species level, yet exhibits a greater divergence in genomic similarity from strains infecting sugar beet and tobacco. Notably, variations were observed in important secretory proteins, such as multiple base deletions in MFS proteins across strains infecting proso millet, rice, and sugar beet.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Discussion: &lt;/strong&gt;Functional analysis of the VAP-1 genome has unveiled a wealth of insights, though we have only begun to scratch the surface. KE","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1557991"},"PeriodicalIF":4.0,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11925929/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143691423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of simulated digestion and prebiotics properties of polysaccharides extracted from <i>Imperatae Rhizoma</i> based on different pilot processes.","authors":"Mengge Sun, Haotian Huang, Haibao Tang, Jiajie Chen, Wei Chen, Dongsheng Yang","doi":"10.3389/fmicb.2025.1544261","DOIUrl":"10.3389/fmicb.2025.1544261","url":null,"abstract":"<p><p>Recent studies have highlighted the prebiotic potential of natural plant polysaccharides, demonstrating their role in promoting beneficial gut microbiota and improving health. However, research on the digestive properties and prebiotic activities of <i>Imperatae Rhizoma Polysaccharides</i> (IRPs) remains limited. This study investigated fresh <i>Imperatae Rhizoma</i> as the research object. After processing, dry <i>Imperatae Rhizoma</i> and carbonized <i>Imperatae Rhizoma</i> were prepared. Three polysaccharides from the fresh, dry, and carbonized <i>Imperatae Rhizoma</i> were extracted with traditional hot water. And another polysaccharide was obtained by cold water extraction from fresh <i>Imperatae Rhizoma</i>. Total four IRPs were extracted and named: IRPs-F, IRPs-D, IRPs-C, and IRPs-J. This study evaluated the prebiotic activity of four polysaccharides derived from the roots of thatch, demonstrating their resistance to digestion, their ability to promote probiotic growth, and their enhancement of short-chain fatty acid (SCFA) production. The final results show that four IRPs exhibit strong resistance to digestion and IRPs-F ability to promote the growth of beneficial probiotics, making it a promising candidate for functional foods aimed at improving intestinal health, immune regulation, and metabolic benefits. This research is highly relevant to food microbiology and holds significant potential for application in the functional food and gut health sectors.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1544261"},"PeriodicalIF":4.0,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11925942/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143691603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Survey of gut microbial biogeography and their functional niche in the grow-finishing swine of ordinary feeding.","authors":"Lili Cao, Wei Guo, Shiyu Yang, Anum Ali Ahmad, Yuntao Dong, Cen Gong, Shuoqi Wang, Xuemin Yang, Zhentao Cheng, Zhihong Yan, Weiwei Wang","doi":"10.3389/fmicb.2025.1530553","DOIUrl":"10.3389/fmicb.2025.1530553","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Background: &lt;/strong&gt;Swine represent one of the most economically significant livestock worldwide, and their intestinal microbial communities are crucial for maintaining physiological development and regulating host metabolism. While extensive research has focused on the fecal microbiota of swine, investigations into microbial communities across different intestinal segments remain limited.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Objective: &lt;/strong&gt;This study aims to elucidate the intestinal microbiota of swine by analyzing luminal contents from different intestinal segments, including the duodenum, jejunum, ileum, cecum, and colon.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;We employed 16S rRNA sequencing to explore the diversity and structure of gut microbial biogeography, microbial functional niches, and their associated pathways.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;Our findings reveal significantly lower microbial richness and diversity in the small intestine (duodenum, jejunum, and ileum) compared to the large intestine (cecum and colon) (&lt;i&gt;p&lt;/i&gt; &lt; 0.05). At the phylum level, Firmicutes, Actinobacteria, Proteobacteria, and Bacteroidetes were the dominant phyla, collectively accounting for over 90% of the total sequences. In the small intestine, Proteobacteria (4.76-34.2%), Actinobacteria, and Fusobacteriota were more abundant, whereas in the large intestine, Firmicutes (89.8-90.4%) was predominated. At the genus level, &lt;i&gt;Fusobacterium&lt;/i&gt;, &lt;i&gt;Corynebacterium&lt;/i&gt;, &lt;i&gt;Rothia&lt;/i&gt;, &lt;i&gt;Bradyrhizobium&lt;/i&gt;, and &lt;i&gt;Brevundimonas&lt;/i&gt; were predominant in duodenum. &lt;i&gt;Romboutsia&lt;/i&gt;, &lt;i&gt;Clostridium_sensu_stricto_1&lt;/i&gt;, &lt;i&gt;Terrisporobacter&lt;/i&gt;, and &lt;i&gt;Jeotgalicoccus&lt;/i&gt; demonstrated greater abundances in the jejunum and ileum. &lt;i&gt;Oscillospiraceae_UCG-005&lt;/i&gt; in the cecum and &lt;i&gt;Christensenellaceae_R-7_group&lt;/i&gt; in the colon were more abundant with 16.4 and 20.2% relative abundances, respectively. The specialists detected from the duodenum to the colon were all the predominant genera in each intestinal segment with relatively higher relative abundance. For instance, &lt;i&gt;Romboutsia&lt;/i&gt; (3.06-36.1%), &lt;i&gt;Clostridium_sensu_stricto_1&lt;/i&gt; (5.31-18.6%), and &lt;i&gt;Terrisporobacter&lt;/i&gt; (0.849-5.72%) were dominant genera and specialists in the small intestine, associated with enriched pathways of Amino acid metabolism and Lipid metabolism. Conversely, &lt;i&gt;Oscillospiraceae_UCG-005&lt;/i&gt; (16.4%, 4.06%) and &lt;i&gt;Christensenellaceae_R-7_group&lt;/i&gt; (5.44%, 20.2%) are predominant genera and specialists within the large intestine, linked to pathways involved in Glycan biosynthesis and metabolism pathway, as well as the Biosynthesis of other secondary metabolites.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Conclusion: &lt;/strong&gt;These highlight the importance of genus specialists compared to genus generalists. The findings provide essential data for assessing the role of the intestinal microbiome in maintaining and enhancing swine health and productivity, offering fundamental guidance for further exploration of host-microbe interaction me","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1530553"},"PeriodicalIF":4.0,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11925874/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143691208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Heterogeneity of rock-hosted microbial communities in a serpentinizing aquifer of the Coast Range Ophiolite.","authors":"Katrina I Twing, William J Brazelton, Tom M McCollom, Florence Schubotz, H Lizethe Pendleton, Rachel L Harris, Annemarie R Brown, Seth M Richins, Michael D Y Kubo, Tori M Hoehler, Dawn Cardace, Matthew O Schrenk","doi":"10.3389/fmicb.2025.1504241","DOIUrl":"10.3389/fmicb.2025.1504241","url":null,"abstract":"<p><p>The movement of groundwater through fractured bedrock provides favorable conditions for subsurface microbial life, characterized by constrained flow pathways and distinctive local environmental conditions. In this study, we examined a subsurface microbial ecosystem associated with serpentinized rocks recovered from the Coast Range Ophiolite in northern California, USA. The distribution and diversity of microbial communities at various depths within two separate cores reaching up to 45 m below the land surface were investigated with microbiological and geochemical approaches. Core samples contained low total organic carbon content, low DNA yields, and low copy numbers of <i>16S rRNA</i> genes, yet some samples still yielded amplifiable DNA sequences. The microbial community composition of rock cores was distinct from groundwater, and source tracking of DNA sequences indicated that groundwater is not a significant source of DNA into basement rocks. In contrast, the microbial community of some rock core samples shared similarities with overlying soil samples, which could indicate potential contamination, weathering of shallow serpentinites, or a combination of both. Individual DNA sequences of archaea and bacteria predicted to be endemic to the basement rocks were identified by differential abundance analyses. Core-enriched sequences were distinct from those in groundwater or in the overlying soils and included OTUs related to <i>Serpentinimonas</i> as well as putatively anaerobic, deep subsurface-associated taxa such as methanogens and <i>Bathyarchaeia</i>. Stable isotope analyses of organic and inorganic carbon did not reveal a chemoautotrophic signal and were instead consistent with a primarily surface vegetation source of organic carbon into the basement. This census of archaeal and bacterial DNA sequences associated with altered ultramafic rocks provides a useful resource for further research into the potential for deep subsurface microbial activity fueled by geochemical reactions associated with serpentinization.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1504241"},"PeriodicalIF":4.0,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11926711/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143691232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intercropping grapevine with <i>Solanum nigrum</i> enhances their cadmium tolerance through changing rhizosphere soil microbial diversity.","authors":"Changbing Pu, Ziyao Huang, Xuefeng Jiang, Jiawei Zhu, Lijin Lin, Xiaoli Zhang, Hui Xia, Dong Liang, Jin Wang, Xiulan Lv","doi":"10.3389/fmicb.2025.1537123","DOIUrl":"10.3389/fmicb.2025.1537123","url":null,"abstract":"<p><strong>Introduction: </strong>Cadmium (Cd), a toxic heavy metal, has increasingly impacted vineyard soils and grapevine (<i>Vitis vinifera</i>) production in recent years. Intercropping with the hyperaccumulator plant <i>Solanum nigrum</i> has emerged as a promising strategy to improve soil health and increase plant resilience to the Cd-contaminated soil.</p><p><strong>Methods: </strong>This study investigated the effects of intercropping grapevine with <i>S. nigrum</i> (IntVVSN) on the soil enzyme activity and microbial community.</p><p><strong>Results: </strong>Compared with the monocultures of <i>S. nigrum</i> and grapevine, IntVVSN increased the activities of soil sucrase, soil urease, and soil cellulase, and decreased the activities of soil amylase, and soil neutral phosphatase. The microbial community in IntVVSN showed higher abundances of beneficial groups such as Acidobacteriota, Actinobacteriota, and Chloroflexi. These groups were involved in the metal detoxification and nutrient cycling, indicating their potential role in enhancing Cd tolerance. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis revealed the distinct metabolic adaptations in IntVVSN under Cd-contaminated soil, with significant upregulation of pathways related to the secondary metabolite synthesis, carbohydrate metabolism, glycan biosynthesis, nucleotide metabolism, and protein processing. The changes in microbial composition, along with the enhanced nutrient cycling indicated by increased soil enzyme activities, suggest a healthier and more resilient soil environment. This, in turn, contributes to improved Cd tolerance in grapevines.</p><p><strong>Conclusion: </strong>This study highlights the phytoremediation potential of <i>S. nigrum</i> intercropping, which promotes sustainable agricultural practices in Cd-contaminated soil by improving plant growth and resilience to heavy metal stress.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1537123"},"PeriodicalIF":4.0,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11925943/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143691239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Lactobacillus johnsonii</i> HL79 modulates the microbiota-gut-brain axis to protect cognitive function in mice chronically exposed to high altitude.","authors":"Zhifang Zhao, Xufei Zhang, Ning Sun, Lixiao Duan, Jinge Xin, Hao Li, Xueqin Ni, Hesong Wang, Hailin Ma, Yang Bai","doi":"10.3389/fmicb.2025.1561400","DOIUrl":"10.3389/fmicb.2025.1561400","url":null,"abstract":"<p><strong>Introduction: </strong>High-altitude environments have significant effects on brain function, particularly a decline in cognitive function, due to insufficient oxygen supply. The microbiome-gut-brain axis (MGBA) plays an important role in regulating cognitive function, but its specific mechanism of action in high-altitude environments is unclear. Therefore, the aim of this study was to investigate whether the probiotic <i>Lactobacillus johnsonii</i> HL79 could alleviate high altitude-induced cognitive dysfunction in mice by modulating the gut microbiota.</p><p><strong>Methods and results: </strong>Sixty C57BL/6 mice aged 8 weeks were randomly divided into four groups: control, high altitude exposure (HA), HL79-treated (P), and high altitude exposure plus HL79-treated (HAP). the HA and HAP groups were exposed to a low-pressure oxygen chamber at a simulated altitude of 3,500-4,000 m for 20 weeks, while the Control and P groups were maintained at the normal barometric pressure level. Probiotic HL79 was given daily by gavage in the P and HAP groups, while saline gavage was given daily in the other two groups. The cognitive functions of the mice were assessed by new object recognition test and elevated plus maze test. The results showed that HL79 treatment significantly improved the working memory abilities of high altitude exposed mice. In addition, HL79 treatment improved antioxidant capacity, decreased malondialdehyde (MDA) content, and increased superoxide dismutase (SOD) and catalase (CAT) activities in serum and whole brain tissue. Gut microbiota analysis showed that HL79 was able to modulate the structure of gut microbiota and increase the relative abundance of beneficial flora in high altitude environment.</p><p><strong>Conclusion: </strong><i>Lactobacillus johnsonii</i> HL79 significantly ameliorated cognitive dysfunction in high altitude-exposed mice by modulating the gut microbiota and antioxidant capacity, further confirming the important role of MGBA in high altitude environment.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1561400"},"PeriodicalIF":4.0,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11925889/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143691577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"COPB1-knockdown induced type I interferon signaling activation inhibits <i>Chlamydia psittaci</i> intracellular proliferation.","authors":"Nana Li, Huiying Yang, Shan Zhang, Yufei Jiang, Yinhui Lin, Xiaoxiao Chen, Yuchen Zhang, Yonghui Yu, Xuan Ouyang, Yujun Cui, Yajun Song, Jun Jiao","doi":"10.3389/fmicb.2025.1566239","DOIUrl":"10.3389/fmicb.2025.1566239","url":null,"abstract":"<p><strong>Objective: </strong><i>Chlamydia psittaci</i> is a zoonotic pathogen that causes an acute disease known as psittacosis. To establish infection in host cells, <i>Chlamydia</i> manipulates the host cell's membrane trafficking pathways.</p><p><strong>Methods: </strong>In this study, using fluorescently labeled <i>C. psittaci</i> and screening a human membrane trafficking small interfering RNA (siRNA) library, we identified 34 host proteins that influenced <i>C. psittaci</i> infection in HeLa cells.</p><p><strong>Results: </strong>Among these, knockdown (KD) of two genes encoding subunits of the coatomer complex I (COPI) inhibited the pathogen's intracellular survival. Specifically, the knockdown of COPB1, a COPI subunit, significantly reduced the intracellular proliferation of <i>C. psittaci</i>. Mechanistically, we found that type I interferon negatively affected <i>C. psittaci</i> infection. Moreover, COPB1 KD disrupted the homeostasis of STING, preventing its retrieval from the Golgi back to the endoplasmic reticulum (ER), which in turn activated type I interferon signaling.</p><p><strong>Conclusion: </strong>Together, our findings advance the understanding of the mechanisms underlying <i>Chlamydia</i> infection and offer potential avenues for the development of new anti-<i>C. psittaci</i> strategies.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1566239"},"PeriodicalIF":4.0,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11922848/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143669528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}