Dietary purslane modulates gut microbiota and fecal metabolites in aging rats.

IF 4 2区生物学 Q2 MICROBIOLOGY

Frontiers in Microbiology Pub Date : 2025-03-19 eCollection Date: 2025-01-01 DOI:10.3389/fmicb.2025.1549853

Jingwen Deng, Xia Wang, Can Yan, Zicheng Huang, Hui Luo, Caihua Dai, Xiaoliu Huang, Yushan Huang, Qiang Fu

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Abstract

Introduction: Portulaca oleracea L. (purslane) is a highly nutritious and edible wild vegetable beneficial to human health. However, its impacts on the structure of gut microbiota and fecal metabolites in aging individuals remain unclear. This study aims to clarify its potential mechanisms in aging-related gut health.

Methods: Naturally aged rats (18 months) were divided into two groups. One group was fed a maintenance chow, and the other was fed a mixture with 3.5% purslane for 15 weeks. Hematoxylin-eosin staining, gas chromatography-mass spectrometry, and 16S rDNA high-throughput sequencing were employed to explore the effects of purslane on the intestinal health of these rats.

Results: The fecal concentrations of acetic acid, propionic acid, butyric acid, valeric acid, caproic acid, and total short-chain fatty acids (SCFAs) were significantly increased in aging rats fed the purslane supplement. Purslane significantly reduced the relative abundance levels of Firmicutes and Fusobacteria, as well as the ratio of Firmicutes to Bacteroidetes. KEGG pathway analysis annotated 109 differential metabolites, which mainly affected metabolic pathways such as linoleic acid metabolism, arachidonic acid metabolism, primary bile acid biosynthesis, steroid biosynthesis, and steroid hormone biosynthesis. There was a strong correlation between Paracbacteroides, the Prevotella NK3B31_group, the Rikenella_RC9_gut_group, and SCFA levels. Aging rats consuming purslane had a more complete and healthy gut morphology than the control group.

Discussion: These results suggested that the maintenance of intestinal health by purslane in aging rats might be associated with the targeted regulation of gut microbiota and fecal metabolites.

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膳食马齿苋调节衰老大鼠肠道微生物群和粪便代谢物。

简介马齿苋（Portulaca oleracea L.）是一种对人体健康有益的高营养可食用野生蔬菜。然而，马齿苋对衰老个体肠道微生物群结构和粪便代谢物的影响仍不清楚。本研究旨在阐明它在与衰老相关的肠道健康中的潜在机制：方法：将自然老龄大鼠（18 个月）分为两组。方法：将自然衰老的大鼠（18 个月）分为两组，一组喂食维持饲料，另一组喂食含有 3.5% 马齿苋的混合饲料，持续 15 周。采用血红素-伊红染色法、气相色谱-质谱法和 16S rDNA 高通量测序法探讨马齿苋对大鼠肠道健康的影响：结果：喂食马齿苋补充剂的老龄大鼠粪便中乙酸、丙酸、丁酸、戊酸、己酸和总短链脂肪酸（SCFAs）的浓度显著增加。马齿苋明显降低了固缩菌和镰刀菌的相对丰度水平，以及固缩菌与类杆菌的比例。KEGG 通路分析注释了 109 个差异代谢物，主要影响亚油酸代谢、花生四烯酸代谢、初级胆汁酸生物合成、类固醇生物合成和类固醇激素生物合成等代谢通路。Paracbacteroides、Prevotella NK3B31_group、Rikenella_RC9_gut_group与SCFA水平之间存在很强的相关性。与对照组相比，食用马齿苋的老年大鼠肠道形态更完整、更健康：这些结果表明，马齿苋对衰老大鼠肠道健康的维护可能与定向调节肠道微生物群和粪便代谢物有关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Frontiers in Microbiology MICROBIOLOGY-

CiteScore

7.70

自引率

9.60%

发文量

4837

审稿时长

14 weeks

期刊介绍： Frontiers in Microbiology is a leading journal in its field, publishing rigorously peer-reviewed research across the entire spectrum of microbiology. Field Chief Editor Martin G. Klotz at Washington State University is supported by an outstanding Editorial Board of international researchers. This multidisciplinary open-access journal is at the forefront of disseminating and communicating scientific knowledge and impactful discoveries to researchers, academics, clinicians and the public worldwide.