Frontiers in Microbiology最新文献

筛选
英文 中文
Non-tuberculous mycobacteria isolates from patients with chronic pulmonary disease and no epidemiological relationship show sequence clusters through whole-genome sequencing.
IF 4 2区 生物学
Frontiers in Microbiology Pub Date : 2025-03-11 eCollection Date: 2025-01-01 DOI: 10.3389/fmicb.2025.1549030
Marc Rubio, Mariana Fernandez-Pittol, Sara Batista, Diego Martínez, Lorena San Nicolas, Elena Portell-Buj, Maria Antònia Busquets, Joan Estelrich, Julian Gonzalez-Martin, Ferran Navarro, Griselda Tudó, Montserrat Garrigó
{"title":"Non-tuberculous mycobacteria isolates from patients with chronic pulmonary disease and no epidemiological relationship show sequence clusters through whole-genome sequencing.","authors":"Marc Rubio, Mariana Fernandez-Pittol, Sara Batista, Diego Martínez, Lorena San Nicolas, Elena Portell-Buj, Maria Antònia Busquets, Joan Estelrich, Julian Gonzalez-Martin, Ferran Navarro, Griselda Tudó, Montserrat Garrigó","doi":"10.3389/fmicb.2025.1549030","DOIUrl":"10.3389/fmicb.2025.1549030","url":null,"abstract":"<p><strong>Objectives: </strong>This study aimed to investigate the genomic epidemiology of slow-growing mycobacteria (SGM) isolates from patients with bronchiectasis through whole-genome sequencing (WGS) and assess various bioinformatic tools to establish relationships between the isolates.</p><p><strong>Methods: </strong>A total of 46 SGM isolates from 37 patients with underlying chronic pulmonary disease, previously identified as <i>Mycobacterium avium</i>, <i>Mycobacterium intracellulare</i>, or <i>Mycobacterium chimaera</i> through polymerase chain reaction, were analyzed using WGS and three different clustering methods, namely rPinecone, Split K-mer analysis (SKA), and custom single nucleotide variant threshold calculation.</p><p><strong>Results: </strong>The three analyses revealed one cluster of <i>M. intracellulare</i> subsp. <i>intracellulare</i> isolates and one cluster of <i>M. intracellulare</i> subsp. <i>chimaera</i> isolates from different patients. The analyses did not indicate any clusters formed by <i>M. avium</i> subsp<i>. avium</i> isolates from different patients.</p><p><strong>Conclusion: </strong><i>M. intracellulare</i> subsp. <i>chimaera</i> and <i>M. intracellulare</i> subsp. <i>intracellulare</i> form clusters of very closely related isolates from patients with no epidemiological relationship. This absence of an epidemiological relationship indicated that the infections were likely acquired from common sources rather than through direct transmission between patients. The use of three methodologies is an adequate strategy for an in-depth study of the relationship between isolates of very closely related species and subspecies.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1549030"},"PeriodicalIF":4.0,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11933053/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143709128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The role of CsrA in controls the extracellular electron transfer and biofilm production in Geobacter sulfurreducens.
IF 4 2区 生物学
Frontiers in Microbiology Pub Date : 2025-03-11 eCollection Date: 2025-01-01 DOI: 10.3389/fmicb.2025.1534446
Alberto Hernández-Eligio, Leticia Vega-Alvarado, Xinying Liu, Jessica Cholula-Calixto, Guillermo Huerta-Miranda, Katy Juárez
{"title":"The role of CsrA in controls the extracellular electron transfer and biofilm production in <i>Geobacter sulfurreducens</i>.","authors":"Alberto Hernández-Eligio, Leticia Vega-Alvarado, Xinying Liu, Jessica Cholula-Calixto, Guillermo Huerta-Miranda, Katy Juárez","doi":"10.3389/fmicb.2025.1534446","DOIUrl":"10.3389/fmicb.2025.1534446","url":null,"abstract":"<p><p>CsrA is a post-transcriptional regulator that controls biofilm formation, virulence, carbon metabolism, and motility, among other phenotypes in bacteria. CsrA has been extensively studied in γ-proteobacteria and firmicutes, However the cellular processes controlled for regulation in δ-proteobacteria remain unknown. In this work, we constructed and characterized the Δ<i>csrA</i> mutant strain in <i>Geobacter sulfurreducens</i> to determine the involvement of the CsrA protein in the regulation of biofilm and extracellular electron transfer. The Δ<i>csrA</i> mutant strain shows higher rates of insoluble Fe(III) reduction than the wild type using acetate as electron donor and the growth with fumarate and soluble (Fe(III)) was similar to wild type. Biofilm quantification and characterization by confocal laser scanning microscopy, showed that the Δ<i>csrA</i> mutant produces up to twice as much biofilm as the wild type strain and more than 95% viable cells. Transcriptome analysis by RNA-seq showed that in Δ<i>csrA</i> biofilms developed on an inert support, differentially expressed 244 genes (103 upregulated and 141 downregulated), including those related to extracellular electron transfer, exopolysaccharide synthesis, c-di-GMP synthesis and degradation. To validate the transcriptome data, RT-qPCR confirmed the differential expression of several selected genes in the Δ<i>csrA</i> strain. Also, current production in microbial fuel cells was performed and the Δ<i>csrA</i> strain produced 45-50% more current than the wild type. To identify the genes that changed expression in the Δ<i>csrA</i> strain in the graphite electrodes in an MFC, a transcriptome analysis was performed 181 genes changed their expression in the Δ<i>csrA</i> biofilms, of which 113 genes were differentially expressed only in MFC and 68 genes changed their expression as well as the transcriptome of biofilms grown on glass. <i>In silico</i> analysis of the 5'-UTR regions revealed that 76 genes that changed expression in the RNA-seq analysis have a consensus sequence for CsrA binding. To our knowledge this is the first report describing the involvement of CsrA in the regulation of extracellular electron transfer and biofilm in a member of the δ-proteobacteria.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1534446"},"PeriodicalIF":4.0,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11934962/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143709275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Significances of miRNAs for predicting sepsis mortality: a meta-analysis.
IF 4 2区 生物学
Frontiers in Microbiology Pub Date : 2025-03-11 eCollection Date: 2025-01-01 DOI: 10.3389/fmicb.2025.1472124
Yuxi Jin, Yue Zhang, Yifei Li, Xiaolan Zheng
{"title":"Significances of miRNAs for predicting sepsis mortality: a meta-analysis.","authors":"Yuxi Jin, Yue Zhang, Yifei Li, Xiaolan Zheng","doi":"10.3389/fmicb.2025.1472124","DOIUrl":"10.3389/fmicb.2025.1472124","url":null,"abstract":"<p><strong>Background: </strong>Sepsis is a life-threatening condition caused by a dysregulated immune response to infection and remains a major cause of mortality in intensive care units (ICUs). Recent studies have identified microRNAs (miRNAs), a class of small RNA molecules, as potential biomarkers for diagnosing and predicting outcomes in sepsis patients. However, the results of these studies have been inconsistent. This meta-analysis aims to comprehensively evaluate the diagnostic and prognostic value of miRNAs in predicting sepsis-related mortality.</p><p><strong>Methods: </strong>A comprehensive literature search was performed across major databases, including PubMed, Cochrane Library, EMBASE, and CNKI, up to April 7, 2024. Data extraction and meta-analysis were conducted using Meta-disk 1.4 and STATA 15.1, employing both fixed- and random-effects models to ensure robust statistical analysis.</p><p><strong>Results: </strong>A total of 55 studies met the inclusion criteria and were analyzed. The pooled sensitivity, specificity, and area under the summary receiver operating characteristic (SROC) curve for miRNA detection were calculated. The overall performance of total miRNA detection demonstrated a sensitivity of 0.76 (95% confidence interval [CI]: 0.74-0.77), a specificity of 0.72 (95% CI: 0.71-0.73), and an SROC value of 0.83. Subgroup analyses revealed that miR-133a-3p exhibited the highest diagnostic accuracy, with a pooled sensitivity of 0.83 (95% CI: 0.70-0.92), specificity of 0.79 (95% CI: 0.71-0.86), and an SROC value of 0.90. Additionally, other miRNAs, including miR-146a, miR-21, miR-210, miR-223-3p, miR-155, miR-25, miR-122, miR-125a, miR-125b, and miR-150, also demonstrated high SROC values (0.84 to 0.76).</p><p><strong>Conclusion: </strong>This meta-analysis underscores the potential of several microRNAs (miRNAs) as reliable biomarkers for predicting sepsis mortality. Specifically, miR-133a-3p, miR-146a, miR-21, miR-210, miR-223-3p, miR-155, miR-25, miR-122, miR-125b, and miR-150 emerge as promising candidates for clinical applications in sepsis prognosis.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1472124"},"PeriodicalIF":4.0,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11933020/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143709210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effects of regional location on the genotype and phenotype of historical Irish brewing yeast.
IF 4 2区 生物学
Frontiers in Microbiology Pub Date : 2025-03-11 eCollection Date: 2025-01-01 DOI: 10.3389/fmicb.2025.1452334
Daniel W M Kerruish, Paul Cormican, Elaine M Kenny, Carl J M Whelan, Steve Gilsenan, Eibhlin Colgan, Katherine A Smart, Chris A Boulton, Sandra N E Stelma
{"title":"Effects of regional location on the genotype and phenotype of historical Irish brewing yeast.","authors":"Daniel W M Kerruish, Paul Cormican, Elaine M Kenny, Carl J M Whelan, Steve Gilsenan, Eibhlin Colgan, Katherine A Smart, Chris A Boulton, Sandra N E Stelma","doi":"10.3389/fmicb.2025.1452334","DOIUrl":"10.3389/fmicb.2025.1452334","url":null,"abstract":"<p><p>Most commercial beers are made using water, malted barley, and hops as the principal ingredients and <i>Saccharomyces</i> yeast as the transforming microorganism. The yeast is used in a semi-conservative process in which crops are collected from one fermentation, stored, and a proportion recycled into a subsequent fermentation. This process differs from wine, cider, and spirit manufacturing where the yeast culture is only used once. The serial fermentation process is continued approximately 8-12 times after which a new culture of verified purity and identity is introduced. This increases the likelihood that the yeast remains true to type. Many commercial brewers use proprietary strains the origins of which are usually unknown. Advances in genetic analyses provide a means for probing the origins of brewing yeast strains, and in this study, six historical Irish brewing yeasts from five breweries located within Ireland were assessed. Using Illumina sequencing technology, whole-genome sequencing data were generated. Single nucleotide polymorphism analysis of these data established that the historical Irish brewing yeast group falls within the previously described \"Britain\" subpopulation Beer 1 clade. Further analysis established that the six historical Irish brewing yeasts separate into two subgroupings, which associated with specific regional locations. Furthermore, the assessment of the six historical Irish brewing yeast phenotypic attributes relevant to brewing correlated within the same regional location groupings. Our data provide further evidence of how brewing requirements associated with specific beer styles have influenced yeast strain selection.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1452334"},"PeriodicalIF":4.0,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11933050/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143709203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Application of compost amended with biochar on the distribution of antibiotic resistance genes in a soil-cucumber system-from the perspective of high-dose fertilization.
IF 4 2区 生物学
Frontiers in Microbiology Pub Date : 2025-03-10 eCollection Date: 2025-01-01 DOI: 10.3389/fmicb.2025.1530296
Shuai Shi, Zhenye Tong, Bo Sun, Yiyang Wei, Yu Tian, Qihui Zuo, Xingxing Qiao, Jiaze Duan, Wenlong Bi, Junmei Qin, Jun Zhou, Fenwu Liu
{"title":"Application of compost amended with biochar on the distribution of antibiotic resistance genes in a soil-cucumber system-from the perspective of high-dose fertilization.","authors":"Shuai Shi, Zhenye Tong, Bo Sun, Yiyang Wei, Yu Tian, Qihui Zuo, Xingxing Qiao, Jiaze Duan, Wenlong Bi, Junmei Qin, Jun Zhou, Fenwu Liu","doi":"10.3389/fmicb.2025.1530296","DOIUrl":"10.3389/fmicb.2025.1530296","url":null,"abstract":"<p><p>The transfer of antibiotic resistance genes (ARGs) from soils to vegetables negatively impacts human health. This study explored the effects of the high-dose (18.73 t/ha) application of traditional compost (TC) and composts produced through the co-composting of traditional materials with large-sized (5-10 mm) biochar-amended compost (LBTC) or small-sized (< 0.074 mm) biochar-amended compost (SBTC) on the distribution of ARGs in a soil-cucumber system were explored. Results indicated that the SBTC group had the highest soil nitrogen, phosphorus, and potassium contents, followed by the LBTC, TC, and control treatment groups. These findings aligned with the quality and weight of harvested cucumbers. Bacterial community diversity decreased in compost-fertilized soils. Compared with their preexperimental values in soils, the total absolute abundances of ARGs and mobile genetic elements (MGEs) increased by 23.88 and 6.66 times, respectively, in the control treatment group; by 5.59 and 5.23 times, respectively, in the TC group; by 5.50 and 1.81 times, respectively, in the LBTC group; and by 5.49 and 0.47 times, respectively, in the SBTC group. Compared with those in the control treatment group, the absolute abundance of <i>ermB</i>, <i>ermT</i>, <i>gyrA</i>, <i>qnrS</i>, <i>tetC</i>, and <i>intI1</i> decreased by 6-100% in the soil of the SBTC group. Compost application to soils significantly decreased ARG abundance in cucumbers; SBTC had the most significant effect and reduced the number of host bacteria at the phylum level from four to three. Nutrient levels in soils were important factors influencing the migration of ARGs from soils to cucumbers. In summary, when compared to other composts, the high-dose (18.73 t/ha) application of SBTC is more effective at reducing the risk of the accumulation and transfer of ARGs in the soil-cucumber system.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1530296"},"PeriodicalIF":4.0,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11931161/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143700123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Amomum tsaoko flavonoids attenuate ulcerative colitis by inhibiting TLR4/NF-κB/NLRP3 signaling pathway and modulating gut microbiota in mice.
IF 4 2区 生物学
Frontiers in Microbiology Pub Date : 2025-03-10 eCollection Date: 2025-01-01 DOI: 10.3389/fmicb.2025.1557778
Zelin Huang, Yan Zhao, Weixing Yang, Chunfen Duan, Jun Sheng, Yang Tian, Lei Peng, Xiaoyu Gao
{"title":"<i>Amomum tsaoko</i> flavonoids attenuate ulcerative colitis by inhibiting TLR4/NF-κB/NLRP3 signaling pathway and modulating gut microbiota in mice.","authors":"Zelin Huang, Yan Zhao, Weixing Yang, Chunfen Duan, Jun Sheng, Yang Tian, Lei Peng, Xiaoyu Gao","doi":"10.3389/fmicb.2025.1557778","DOIUrl":"10.3389/fmicb.2025.1557778","url":null,"abstract":"<p><p>Ulcerative colitis (UC) is a global disease for which there is little of effective treatment options, thus creating an urgent need for the development of new drug candidates from natural and functional foods. <i>Amomum tsaoko</i> Crevost et Lemarie is a kind of medicinal and food herb that is rich in flavonoids. However, the pharmacological effects of <i>Amomum tsaoko</i> flavonoids (ATF) on UC have not yet been reported. The present study established a mouse model of UC using 3% dextran sulfate sodium (DSS), and modern molecular biology techniques such as IHC, RT-qPCR, Western blot, and 16S rRNA gene analysis were used to investigate the effect of ATF in UC mice. The results demonstrated that a high dose of ATF (100 mg/kg) led to a significant restoration of body weight, disease activity index score, and colon length, in addition to ameliorating colonic tissue damage in UC mice. ATF reduced the serum levels of lipopolysaccharides (LPS), inhibited the activation of the colonic TLR4/NF-κB/NLRP3 signaling pathway, and increased the mRNA expression of tight junction proteins such as <i>ZO-1</i>, <i>Occludin</i>, and <i>Claudin4</i>. Furthermore, ATF was found to reduce the relative abundance of the DSS-induced conditional pathogenic (<i>Escherichia-Shigella</i>, <i>Colidextribacter</i>, and <i>Oscillibacter</i>), increase the potential probiotic taxa (<i>Akkermansia</i>, <i>Bifidobacterium</i> and <i>unclassified_f__Atopobiaceae</i>). Interestingly, these genera were found to be significantly correlated with the UC core phenotypic indicators. These findings indicated that ATF may alleviate UC symptoms by modulating the gut microbiota-LPS/TLR4/NF-κB/NLRP3 axis. The present study has the potential to serve as a valuable reference point for understanding the role of natural flavonoids in the prevention of inflammatory diseases, and to expand the future applications of ATF in the fields of food and medicine.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1557778"},"PeriodicalIF":4.0,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11932354/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143700118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Differentiated fatty acid allocation of Daphnia magna helped to maintain their population under food quality deterioration.
IF 4 2区 生物学
Frontiers in Microbiology Pub Date : 2025-03-10 eCollection Date: 2025-01-01 DOI: 10.3389/fmicb.2025.1544005
Sirui Wang, Zhengwen Liu, Xiaoqi Su, Xiaotong Jin, Hui Jin, Yaling Su, Jianjun Wang, Erik Jeppesen, Xiufeng Zhang, Yali Tang
{"title":"Differentiated fatty acid allocation of <i>Daphnia magna</i> helped to maintain their population under food quality deterioration.","authors":"Sirui Wang, Zhengwen Liu, Xiaoqi Su, Xiaotong Jin, Hui Jin, Yaling Su, Jianjun Wang, Erik Jeppesen, Xiufeng Zhang, Yali Tang","doi":"10.3389/fmicb.2025.1544005","DOIUrl":"10.3389/fmicb.2025.1544005","url":null,"abstract":"<p><p>Polyunsaturated fatty acids (PUFAs) are vital to the physiological functioning of crustacean zooplankton. However, cyanobacteria blooms frequently lead to PUFA deficiencies, which poses a substantial challenge to population fitness. Therefore, we hypothesize that <i>D. magna</i> adapt to PUFA-deficient conditions by prioritizing PUFA allocation to somatic growth, and then to offspring during reproduction to ensure population persistence. To test this hypothesis, we applied (compound-specific) <sup>13</sup>C labeling to compare the turnover of total carbon and certain groups of fatty acids in <i>Daphnia magna</i> fed with <i>Scenedesmus bijuba</i> for 6 days and then switching to a diet of <sup>13</sup>C labeled <i>Microcystis wesenbergii</i> for 6 days (with food quality deterioration) or to a diet of <sup>13</sup>C-labeled <i>Scenedesmus</i> (without food quality deterioration), respectively. Fatty acid profiles of <i>D. magna</i> mothers and offspring were also analyzed to reveal their PUFA allocation strategies. Life table parameters from <i>D. magna</i>-feeding <i>Scenedesmus</i> switching to <i>Microcystis</i> were compared with <i>D. magna</i> fed with only <i>Scenedesmus</i> or <i>Microcystis</i> to reveal the effect of PUFA allocation on <i>D. magna</i> performance. Our results showed that with food quality deterioration, <i>D. magna</i> exhibited a significantly lower PUFA and carbon turnover and higher offspring: mother ratios in their PUFA contents. Despite this reduced reproduction, the <i>D. magna</i> switching diets showed no significant different intrinsic increasing rate of populations with those fed only <i>Scenedesmus.</i> Meanwhile, the <i>D. magna</i> switching diets performed significantly better than <i>D. magna</i> fed only <i>Microcystis</i>. These results suggest that differential fatty acid allocation of consumers may serve as an adaptive strategy for population maintenance in food quality deterioration and provide ecological implications with cyanobacterial bloom management and <i>Daphnia</i> reproductive plasticity, which needs further explorations.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1544005"},"PeriodicalIF":4.0,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11931139/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143700127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Research status of the relationship between microecological imbalance and lung cancer.
IF 4 2区 生物学
Frontiers in Microbiology Pub Date : 2025-03-10 eCollection Date: 2025-01-01 DOI: 10.3389/fmicb.2025.1558379
Xin Jin, Yangang Gu, Xiaojie Song
{"title":"Research status of the relationship between microecological imbalance and lung cancer.","authors":"Xin Jin, Yangang Gu, Xiaojie Song","doi":"10.3389/fmicb.2025.1558379","DOIUrl":"10.3389/fmicb.2025.1558379","url":null,"abstract":"<p><p>Microecology refers to the ecosystem formed by human and microbial communities in the process of co-evolution, the microecological imbalance is associated with occurrence and development of multiple diseases, including lung cancer. In this review, we detailedly summarized the concept and roles of microecology, the relationship between microecology and human diseases, and related techniques in microecology studies. Importantly, we specially analyzed the correlations between microecology and lung cancer by focusing on gut microbiota, oral microbiota and lower respiratory tract microbiota, and further evaluated the effects of microbiota dysbiosis on chemotherapy and immunotherapy efficacy in lung cancer. At last, we discussed the potential mechanisms by which dysregulated microbiota promotes the genesis and development of lung cancer. Microecology-centered detection and intervention will improve the early diagnosis of lung cancer and provide new targets for the treatment of lung cancer.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1558379"},"PeriodicalIF":4.0,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11931131/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143700132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Efficacy of dispirotripiperazine PDSTP in a golden Syrian hamster model of SARS-CoV-2 infection.
IF 4 2区 生物学
Frontiers in Microbiology Pub Date : 2025-03-10 eCollection Date: 2025-01-01 DOI: 10.3389/fmicb.2025.1546946
Giuseppina Sanna, Olga Riabova, Elena Kazakova, Alexander Lepioshkin, Natalia Monakhova, Alessandra Marongiu, Gianluigi Franci, Aldo Manzin, Vadim Makarov
{"title":"Efficacy of dispirotripiperazine PDSTP in a golden Syrian hamster model of SARS-CoV-2 infection.","authors":"Giuseppina Sanna, Olga Riabova, Elena Kazakova, Alexander Lepioshkin, Natalia Monakhova, Alessandra Marongiu, Gianluigi Franci, Aldo Manzin, Vadim Makarov","doi":"10.3389/fmicb.2025.1546946","DOIUrl":"10.3389/fmicb.2025.1546946","url":null,"abstract":"<p><p>The increasing incidence of viral pandemics calls for new small-molecule therapeutics beyond traditional approaches and targets. Dispirotripiperazine, composed of two positively charged nitrogen atoms, represents an unusual scaffold in drug discovery campaigns, and molecules based on it are known to prevent virus infection by disrupting early host-pathogen interactions. In this study, the adhesion-blocking dispirotripiperazine core compound PDSTP was evaluated against SARS-CoV-2 <i>in vitro</i> and <i>in vivo</i>. We demonstrated that the molecule was acceptably active against two clinical isolates affecting the early stages of the SARS-CoV-2 cycle. In a hamster model of SARS-CoV-2 pneumonia, PDSTP treatment resulted in reduced viral loads in the lungs and turbinates and milder lung tissue lesions. Overall, these data support PDSTP as a preclinical candidate for the treatment of COVID-19.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1546946"},"PeriodicalIF":4.0,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11931052/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143700129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Kovacikia euganea sp. nov. (Leptolyngbyaceae, Cyanobacteria), a new chlorophyll f producing cyanobacterium from the Euganean Thermal District (Italy).
IF 4 2区 生物学
Frontiers in Microbiology Pub Date : 2025-03-10 eCollection Date: 2025-01-01 DOI: 10.3389/fmicb.2025.1545008
Raffaella Margherita Zampieri, Edoardo Bizzotto, Stefano Campanaro, Fabrizio Caldara, Micol Bellucci, Nicoletta La Rocca
{"title":"<i>Kovacikia euganea</i> sp. nov. (Leptolyngbyaceae, Cyanobacteria), a new chlorophyll <i>f</i> producing cyanobacterium from the Euganean Thermal District (Italy).","authors":"Raffaella Margherita Zampieri, Edoardo Bizzotto, Stefano Campanaro, Fabrizio Caldara, Micol Bellucci, Nicoletta La Rocca","doi":"10.3389/fmicb.2025.1545008","DOIUrl":"10.3389/fmicb.2025.1545008","url":null,"abstract":"<p><p>Hot springs are considered modern terrestrial environments analogous to Archean continental surfaces, where photosynthetic life could have evolved. In this habitat cyanobacteria dominate thanks to the adaptations to high temperature and the capability to acclimate to low light intensity and far-red enriched spectra typical of microbial biofilms. The isolation and characterization of new cyanobacterial species from these environments is fundamental to discover genetic and physiological traits allowing them to thrive under such unfavorable conditions, giving useful information to understand the evolution and plasticity of oxygenic photosynthesis as well as to assess their metabolic biodiversity for biotechnological purposes. In this study, we present the polyphasic characterization of a filamentous cyanobacterium, denominated strain ETS-13, isolated from mud biofilms collected in the Euganean Thermal District (Italy). The area is known since ancient times for the presence of thermal springs and muds exploited for the beneficial properties linked to heat, electrolytes, and organic compounds produced by the microbiota. The ETS-13 genome was assembled and annotated, while phylogenetic analyzes were performed using a combined approach based on the 16S rRNA sequence and considering the 16S-23S ITS secondary structures. In addition, morphological, biochemical, and physiological features of the organism were investigated, allowing its classification as a new species of the <i>Kovacikia</i> genus, named <i>Kovacikia euganea</i>, which formed a cluster with other species of Leptolyngbyaceae from thermal environments. Interestingly, the strain was the first isolated in Italy capable of performing Far-Red Light Photoacclimation (FaRLiP) when exposed to far-red light, a feature found in other species of the same genus so far tested for this acclimation and isolated form geographically distant and different environments.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1545008"},"PeriodicalIF":4.0,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11931122/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143700120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
相关产品
×
本文献相关产品
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信