Frontiers in Microbiology最新文献

{"title":"Exploring the multifunctionality of myco-synthesized selenium nanoparticles: biological, docking study and plant growth-promoting perspectives.","authors":"Hana Sonbol, Eman Zakaria Ahmed, Eslam T Mohamed, Asmaa F Abdelmonem, Heba El-Sayed","doi":"10.3389/fmicb.2025.1565907","DOIUrl":"10.3389/fmicb.2025.1565907","url":null,"abstract":"<p><p>Selenium is a vital trace mineral that regulates essential physiological functions, and the development of sustainable methods for synthesizing selenium nanoparticles (SeNPs) is an active area of research. This study reported the mycosynthesis of SeNPs using the aqueous extract of the edible mushroom <i>Pleurotus ostreatus</i>. The synthesized SeNPs were characterized using various analytical techniques, including UV-visible spectroscopy, dynamic light scattering, transmission electron microscopy (TEM), and Fourier-transform infrared spectroscopy (FTIR). The results revealed that the SeNPs exhibited spherical morphology with a diameter range of 72-148 nm, moderate stability with a zeta potential of -10.5 mV, and a polydispersity index of 0.27. The myco-synthesized SeNPs demonstrated potent antioxidant activity with a DPPH radical scavenging IC₅₀ value of 662.1 ± 1.05 μg/mL, comparable to the standard antioxidant Trolox (IC₅₀ = 24.42 ± 0.87 μM). Furthermore, SeNPs exhibited considerable antimicrobial efficacy against <i>Staphylococcus aureus</i> (17 ± 0.02 mm inhibition zone), followed by <i>Escherichia coli</i> (16 ± 1.04 mm) and <i>Candida albicans</i> (12 ± 0.3 mm). Additionally, SeNPs displayed moderate antiviral activity against a low-pathogenic coronavirus (229E) strain, with a selectivity index (SI) of 5. In agriculture, the application of SeNPs at 10 μM significantly increased primary metabolite production in wheat (<i>Triticum aestivum</i>), with total soluble sugars reaching 54.32 mg/g and soluble proteins increasing to 139.66 mg/g, promoting both shoot and root growth. The comprehensive characterization and evaluation of SeNPs provide valuable insights into their multifunctionality, paving the way for further exploration in medicine, agriculture, and environmental applications.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1565907"},"PeriodicalIF":4.0,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11952769/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143751981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gut microbiota community and metabolic profiles in direct total cavopulmonary connection and Fontan circulation: a cross-sectional study in the single center.","authors":"Kaiyu Wang, Linjiang Han, Jianrui Ma, Yushen Fang, Yinru He, Xiaobing Liu, Shusheng Wen, Jian Zhuang, Haiyun Yuan","doi":"10.3389/fmicb.2025.1539046","DOIUrl":"10.3389/fmicb.2025.1539046","url":null,"abstract":"<p><strong>Background: </strong>This study aimed to evaluate the impact of the direct total cavopulmonary connection (dTCPC) procedure on the gut microbiome and metabolome. It also sought to elucidate the features of the gut microbiota community and metabolic profiles in Fontan circulation.</p><p><strong>Methods: </strong>We randomly recruited 45 participants above 14 years old undergoing Fontan procedure by typical extracardiac conduit (TCPC group, <i>n</i> = 15), direct total cavopulmonary connection (dTCPC group, <i>n</i> = 16) procedure and healthy control (control group, <i>n</i> = 14) in our institution between May 2023 and October 2023. 16S rRNA amplicon sequencing and untargeted metabolites measurement were performed on their fecal sample.</p><p><strong>Results: </strong>The four alpha diversity indexes showed no statistical significance between the dTCPC and TCPC groups (<i>p</i> > 0.05). Orthogonal partial least squares discriminant analysis (OPLS-DA) followed by permutation testing indicated an overfitting effect in the model between the dTCPC and TCPC groups. We observed significant differences in the Chao1 index (<i>p</i> = 0.0236), the ACE index (<i>p</i> = 0.0236), and the unweighted beta diversity (<i>p</i> = 0.0099) between the Fontan group and healthy control group. Strains of <i>Fusobacterium</i> were significantly overrepresented in the Fontan group [with linear discriminant analysis (LDA) scores exceeding (log10) >3]. Functional enrichment analysis revealed a significant overrepresentation of several metabolic pathways. These pathways predominantly included those related to amino acid metabolism, such as histidine metabolism, glycine, serine, and threonine metabolism, and cysteine and methionine metabolism. Additionally, the biosynthesis of unsaturated fatty acids was also notably enriched.</p><p><strong>Conclusion: </strong>The dTCPC procedure demonstrated similar gut microbiota composition and metabolic profiles to the traditional ECC procedure in Fontan patients. Notably, the increased abundance of <i>Fusobacterium</i>, reduced microbial biodiversity, and altered metabolic profiles of amino acids and unsaturated fatty acids in the alimentary canal may serve as distinctive characteristics of patients who have undergone Fontan circulation. These findings provide valuable insights into the long-term physiological consequences of Fontan procedure and may inform future clinical management strategies.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1539046"},"PeriodicalIF":4.0,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11952763/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143751983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Human papillomavirus self-sampling in Asia: a systematic review.","authors":"Xuechao Ji, Menglin Hao, Yixiao Wang, Wenzhi Kong, Zangyu Pan, Qi Sun, Jinwei Miao","doi":"10.3389/fmicb.2025.1540609","DOIUrl":"10.3389/fmicb.2025.1540609","url":null,"abstract":"<p><strong>Background: </strong>Human papillomavirus (HPV) self-sampling may be an accurate and effective alternative sampling method to conventional cervical cancer screening methods. This systematic review compares the accuracy and acceptance of self-sampling to clinician sampling for HPV testing in Asia.</p><p><strong>Methods: </strong>The PubMed, Cochrane Library, Cumulative Index to Nursing and Allied Health, and Web of Science databases were searched for publications published from the establishment of the database to 2023. The risk of bias was assessed using the QUADAS-2 tool for studies included in this review. All studies evaluating the accuracy and acceptance of HPV self-sampling, and agreement of self- and clinician-collected samples in Asia were included. The accuracy of each study was demonstrated through the sensitivity and specificity in diagnosing cervical intraepithelial neoplasia or cancer, as well as the detection rate of HPV. The agreement between the two sampling methods was assessed based on the detection outcomes of HPV. Acceptance was indicated by women's preferences for HPV self-sampling.</p><p><strong>Results: </strong>Sixty-seven studies including 117,279 adult, female participants were included in this review. The type of HPV screening, other intervention components, study design, sample size, follow-up period, analysis method, numerical outcomes, results, and limitations were extracted from each study. The sensitivity and specificity of HPV self-sampling in detecting cervical intraepithelial neoplasia were higher than 80% and 70%, consistent with the results of HPV clinician sampling. The consistency between self-sampling and clinician-sampling was high in most studies, and the kappa value was more than 0.7. Women had high acceptance of self-sampling but expressed some concerns.</p><p><strong>Conclusion: </strong>Self-sampling for HPV testing can significantly improve cervical cancer screening coverage, especially in areas with limited medical resources or reluctance to accept physician sampling. In most studies, the accuracy and acceptance of HPV self-sampling was comparable to clinician sampling. However, the diagnostic criteria and HPV detection methods still need to be adjusted due to the low sensitivity of HPV self-sampling in some studies in China and India. Targeted health education should be carried out to improve the acceptance of HPV self-sampling in women.</p><p><strong>Systematic review registration: </strong>https://inplasy.com/?s=INPLASY202520107, INPLASY202520107.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1540609"},"PeriodicalIF":4.0,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11949917/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143751997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Erratum: Diurnal temperature variation in surface soils: an underappreciated control on microbial processes.","authors":"","doi":"10.3389/fmicb.2025.1586779","DOIUrl":"https://doi.org/10.3389/fmicb.2025.1586779","url":null,"abstract":"<p><p>[This corrects the article DOI: 10.3389/fmicb.2024.1423984.].</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1586779"},"PeriodicalIF":4.0,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11949417/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143729617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intervention and research progress of gut microbiota-immune-nervous system in autism spectrum disorders among students.","authors":"Min Zhou, Baoming Niu, Jiarui Ma, Yukang Ge, Yanxin Han, Wenrui Wu, Changwu Yue","doi":"10.3389/fmicb.2025.1535455","DOIUrl":"10.3389/fmicb.2025.1535455","url":null,"abstract":"<p><p>Autism Spectrum Disorder (ASD) is a neurodevelopmental disorder characterized by difficulties in social interaction and communication, repetitive and stereotyped behaviors, restricted interests, and sensory abnormalities. Its etiology is influenced by both genetic and environmental factors, with no definitive cause identified and no specific pharmacological treatments available, posing a significant burden on patients' families and society. In recent years, research has discovered that gut microbiota dysbiosis plays a crucial role in the pathogenesis of ASD. The gut microbiota can influence brain function and behavior through the gut-brain axis via the nervous system, immune system, and metabolic pathways. On the one hand, specific gut microbes such as <i>Clostridium</i> and <i>Prevotella</i> species are found to be abnormal in ASD patients, and their metabolic products, like short-chain fatty acids, serotonin, and GABA, are also involved in the pathological process of ASD. On the other hand, ASD patients exhibit immune system dysfunction, with gut immune cells and related cytokines affecting neural activities in the brain. Currently, intervention methods targeting the gut microbiota, such as probiotics, prebiotics, and fecal microbiota transplantation, have shown some potential in improving ASD symptoms. However, more studies are needed to explore their long-term effects and optimal treatment protocols. This paper reviews the mechanisms and interrelationships among gut microbiota, immune system, and nervous system in ASD and discusses the challenges and future directions of existing research, aiming to provide new insights for the prevention and treatment of ASD.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1535455"},"PeriodicalIF":4.0,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11936958/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143718252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of microbiota in the chronic prostatitis/chronic pelvis pain syndrome: a review.","authors":"An-Qi Deng, Shao-Yu Yue, Di Niu, Dan-Dan Zhang, Bing-Bing Hou, Li Zhang, Chao-Zhao Liang, He-Xi Du","doi":"10.3389/fmicb.2025.1488732","DOIUrl":"10.3389/fmicb.2025.1488732","url":null,"abstract":"<p><p>Chronic prostatitis/Chronic pelvis pain syndrome (CP/CPPS), a kind of frequent urinary condition among adult males, has caused a lot of inconvenience to patients in life, whose pathogenesis is unclear. Current evidence suggests that it is most likely to be an autoimmune disease. Symbiotic microbes, a highly diverse biological community that harbors trillions of microbes in each region of the human body, have gradually made people realize their important role in immune regulation, material metabolism, and health maintenance. In recent years, increasing studies have shown a connection between microbiota and CP/CPPS. In view of this, we performed this review to summarize the literature pertaining to microbiota and its association with the pathophysiological mechanism of CP/CPPS. In addition, we gleaned the latest progress in the therapeutic strategy of CP/CPPS that related to microbiota regulation in order to offer new perspectives on the management of CP/CPPS.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1488732"},"PeriodicalIF":4.0,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11937130/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143718361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of weather and seasonal factors on whitefly dynamics, associated endosymbiotic microbiomes, and <i>Begomovirus</i> transmission causing tomato leaf curl disease: insights from a metagenomic perspective.","authors":"S Sujatha, Kopparthi Amrutha Valli Sindhura, Prasanna S Koti, Shridhar Hiremath, Mantesh Muttappagol, H D Vinay Kumar, K S Shankarappa, V Venkataravanappa, K M Srinivas Reddy, C N Lakshminarayana Reddy","doi":"10.3389/fmicb.2025.1555058","DOIUrl":"10.3389/fmicb.2025.1555058","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Introduction: &lt;/strong&gt;&lt;i&gt;Bemisia tabaci&lt;/i&gt; (Gennadius) is a globally significant agricultural pest, responsible for transmitting over 120 plant viruses, including those from the Begomovirus genus, which contribute to considerable crop losses. The species complex comprises cryptic species, associated with a diverse array of bacterial endosymbionts that play essential roles in host nutrition, virus transmission, and overall host adaptability. These endosymbionts are classified into primary and secondary categories, with primary endosymbionts forming obligatory, long-term associations, and secondary endosymbionts influencing factors such as biotype differentiation and vector competency. Notably, these microbial communities enhance &lt;i&gt;B. tabaci&lt;/i&gt;'s capacity to transmit viruses, including the tomato leaf curl virus (ToLCuV), which poses a significant threat to tomato production.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;In this study, we examined the population dynamics of &lt;i&gt;B. tabaci&lt;/i&gt; across three major tomato-growing regions in Karnataka, South India, focusing on their seasonal associations with endosymbionts and the incidence of tomato leaf curl disease (ToLCuD). Multiple regression analysis was employed to assess the influence of weather parameters on whitefly populations and disease prevalence. Additionally, we constructed a metagenomic profile to evaluate the effects of geographical location, seasonality, environmental factors, and agricultural practices on the bacterial communities associated with &lt;i&gt;B. tabaci&lt;/i&gt;. Species-specific primers were used to validate the presence and diversity of these bacterial communities.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;Meteorological data revealed a positive correlation between temperature and &lt;i&gt;B. tabaci&lt;/i&gt; populations, which corresponded with an increased incidence of ToLCuD. Genetic characterization of the whitefly identified Asia II-5 and Asia II-7 cryptic species as the dominant forms in the surveyed regions, with Portiera emerging as the most prevalent endosymbiont. A more in-depth analysis of the microbial communities associated with &lt;i&gt;B. tabaci&lt;/i&gt;, utilizing 16S rRNA metagenomic sequencing, revealed a dominance of the Proteobacteria phylum. The endosymbiotic bacterial consortium was primarily composed of &lt;i&gt;Candidatus Portiera&lt;/i&gt;, &lt;i&gt;Candidatus Hamiltonella&lt;/i&gt;, &lt;i&gt;Candidatus Rickettsia&lt;/i&gt;, and &lt;i&gt;Candidatus Arsenophonus&lt;/i&gt;.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Discussion: &lt;/strong&gt;The metagenomic analysis revealed a highly diverse array of bacterial communities, with 92% of sequences classified under Proteobacteria, representing a spectrum of microbial types associated with &lt;i&gt;B. tabaci&lt;/i&gt; ranging from parasitic and pathogenic to mutualistic. Within this phylum, Alphaproteobacteria were predominant, known for their role as facultative symbionts, while Gammaproteobacteria provided essential nutrients to arthropods, enhancing their survival and fitness. The interplay of continuous and intensive tomato cultivatio","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1555058"},"PeriodicalIF":4.0,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11936956/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143718247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbiome analysis reveals dynamic changes of gut microbiota in Guizhou horse and Dutch Warmblood horses.","authors":"Yanfang Lan, Yaonan Li, Yang Wang","doi":"10.3389/fmicb.2025.1562482","DOIUrl":"10.3389/fmicb.2025.1562482","url":null,"abstract":"<p><p>In recent years, the importance of gut microbiota in digestive absorption, metabolism, and immunity has garnered increasing attention. China possess abundant horse breed resources, particularly Guizhou horses, which play vital roles in local agriculture, tourism, and transportation. Despite this, there is a lack of comparative studies on the gut microbiota of native Guizhou horses (GZH) and imported Dutch Warmblood horses (WH). To address this gap, fecal samples were collected from both GZH and WH, and 16S rRNA high-throughput sequencing was utilized to analyze the differences in their gut microbiota. The results indicated that compared with GZH, the abundance of the gut bacterial community in WH was significantly higher, whereas the abundance of the gut fungal community was lower. Furthermore, PCoA-based scatter plot analysis demonstrated distinct differences in the structure of gut bacteria and fungi between the two breeds. While both types of horses share similar major bacterial and fungal phyla, significant differences were observed in numerous bacterial and fungal genera. Moreover, functional predictions of gut bacterial communities suggested that WH exhibit a more robust digestive system and enhanced glycan biosynthesis and metabolism capabilities. This is the first report on the comparative analysis of the gut microbiota in GZH and WH. The results emphasize the significant differences in gut microbiota among various horse breeds and offer valuable insights into the composition and structure of gut microbiota in different horse breeds.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1562482"},"PeriodicalIF":4.0,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11936890/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143718298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The potential of the antifungal nystatin to be repurposed to fight the protozoan <i>Trypanosoma cruzi</i>.","authors":"Belén Jesús Maciel, Chantal Reigada, Fabio Augusto Digirolamo, Marcos Rengifo, Claudio Alejandro Pereira, Mariana Reneé Miranda, Melisa Sayé","doi":"10.3389/fmicb.2025.1539629","DOIUrl":"10.3389/fmicb.2025.1539629","url":null,"abstract":"<p><p>Chagas disease, caused by the parasite <i>Trypanosoma cruzi</i>, affects 6 million people worldwide. Although the drugs benznidazole (BZN) and nifurtimox are available to treat Chagas, they are not effective in the chronic phase when most patients are diagnosed. Moreover, long-term regimen and severe side effects often lead to poor adherence and treatment abandonment. These problems highlight the urgent need to develop new therapies to treat this neglected disease. Given that the antifungal drug nystatin (NYS) affects arginine uptake in yeasts, and fluctuations on arginine availability through transport processes in <i>T. cruzi</i> can negatively affect its viability, in this work we evaluated the potential of NYS for drug repurposing against <i>T. cruzi</i>. NYS inhibited arginine uptake and presented trypanocidal effect in both epimastigotes (IC50 0.17 μM) and trypomastigotes (IC50 4.90 μM). In addition, treatment of infected cells with NYS decreased the release of trypomastigotes with better efficacy than BZN (IC50s 4.83 μM and 8.60 μM, respectively) suggesting that NYS affects the progression of the intracellular life cycle. Furthermore, we observed a synergistic effect both in isolated trypomastigotes and infected cells when NYS was combined with BZN, which could enhance efficacy while improving treatment safety and adherence. As in yeasts, the mechanism of action of NYS in <i>T. cruzi</i> involved the plasma membrane disruption, and membrane transport processes, like amino acids and thymidine uptake, were affected prior to the disruption probably due to NYS interaction with the membrane. Drug repurposing is a recommended strategy by the World Health Organization to develop new therapeutic alternatives for neglected diseases. Our results indicate that NYS presents great potential to be repurposed as a trypanocidal drug to fight <i>T. cruzi</i>.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1539629"},"PeriodicalIF":4.0,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11937040/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143718357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Akkermansia muciniphila</i> helps in the recovery of lipopolysaccharide-fed mice with mild intestinal dysfunction.","authors":"Yue Hu, Jun Zhou, Xiaoqi Lin","doi":"10.3389/fmicb.2025.1523742","DOIUrl":"10.3389/fmicb.2025.1523742","url":null,"abstract":"<p><strong>Background: </strong>Mild intestinal dysfunction, linked to subtle yet significant health issues, can be induced by lipopolysaccharide (LPS), a Gram-negative bacterial component that disrupts gut function and triggers inflammation. <i>Akkermansia muciniphila</i> has shown promise as a probiotic for gut health due to its roles in mucin degradation and short-chain fatty acid production. This study explores the therapeutic effects of <i>Akkermansia muciniphila</i> on LPS-induced mild intestinal dysfunction in mice.</p><p><strong>Methods: </strong>Thirty-eight 6-week-old C57BL/6 mice were split into control (<i>n</i> = 19) and LPS-treated (<i>n</i> = 19) groups. LPS-treated mice received 300 μg/kg/day of LPS for 4 weeks, followed by <i>Akkermansia muciniphila</i> supplementation at 41 mg/kg/day (Akk1) or 82 mg/kg/day (Akk2) for another 4 weeks. Gut microbiota was analyzed via metagenomic sequencing, and gene expression was evaluated through transcriptomics.</p><p><strong>Results: </strong>LPS significantly altered gut microbiota, reducing diversity and increasing pathogenic genera like Lachnoclostridium. <i>Akkermansia muciniphila</i> supplementation, particularly at higher doses, partially restored gut microbiota by increasing beneficial genera such as Muribaculum. Transcriptomics showed that LPS induced immune and inflammatory responses, while <i>Akkermansia muciniphila</i> reduced these effects by modulating pathways like TNF and NF-kappa B signaling.</p><p><strong>Conclusion: </strong><i>Akkermansia muciniphila</i> mitigates LPS-induced gut dysfunction by restoring microbiota balance and modulating immune responses, highlighting its potential as a therapeutic agent for gut health.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"16 ","pages":"1523742"},"PeriodicalIF":4.0,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11938125/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143718132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}