Frontiers in Microbiology最新文献

{"title":"Editorial: Soilborne pathogenic fungi: systematics, pathogenesis and disease control.","authors":"Shitou Xia, W G Dilantha Fernando, Junxing Lu","doi":"10.3389/fmicb.2024.1525583","DOIUrl":"10.3389/fmicb.2024.1525583","url":null,"abstract":"","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"15 ","pages":"1525583"},"PeriodicalIF":4.0,"publicationDate":"2024-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11659278/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142876398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"One-pot MCDA-CRISPR-Cas-based detection platform for point-of-care testing of severe acute respiratory syndrome coronavirus 2.","authors":"Xiaoxia Wang, Rui Yang, Tian Tang, Yuzhen Zhou, Heng Chen, Yihao Jiang, Shirong Zhang, Sihan Qin, Meijuan Wang, Chuan Wang","doi":"10.3389/fmicb.2024.1503356","DOIUrl":"10.3389/fmicb.2024.1503356","url":null,"abstract":"<p><p>Compared to quantitative real-time PCR (q-PCR), CRISPR-Cas-mediated technology is more suitable for point-of-care testing (POCT) and has potential for wider application in the future. Generally, the operational procedure of CRISPR-Cas-mediated diagnostic method consists of two independent steps, the reaction of signal amplification and the CRISPR-Cas-mediated signal detection. Complex multi-step procedures can easily lead to cross-contamination. To develop a convenient and rapid method for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) detection, we propose a MCTOP method (<i>M</i>ultiple cross displacement amplification-<i>C</i>RISPR-Cas12b-based <i>t</i>esting in <i>o</i>ne-<i>p</i>ot), which targets the open reading frame 1ab (ORF1ab) and nucleocapsid protein (N) gene of SARS-CoV-2. This method combines MCDA isothermal amplification and CRISPR-Cas-mediated sequence-specific detection into a one-pot reaction. The optimal reaction was achieved with isothermal amplification of 40 min and CRISPR-Cas-based detection of 15 min, both at 64°C. Then, the results can be visualized by the real-time fluorescence instrument and also lateral flow biosensor. The lowest detection limit of the proposed method is 10 copies of each of target sequences, and it has no cross-reactivity with non-SARS-CoV-2 templates. In a clinical test of 70 pharyngeal swab samples, MCTOP assay showed a specificity of 100% and sensitivities of 98 and 96% for the real-time fluorescence instrument and lateral flow biosensor, respectively. The MCTOP developed in this study is a rapid, convenient, highly sensitive, and specific method for SARS-CoV-2 nucleic acid detection. It can be used as an effective point-of-care testing (POCT) tool for clinical diagnosis and epidemiologic surveillance of SARS-CoV-2 infections, especially suitable for the basic, field and clinical laboratory.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"15 ","pages":"1503356"},"PeriodicalIF":4.0,"publicationDate":"2024-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11659237/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142876595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"β-lactam antibiotics induce metabolic perturbations linked to ROS generation leads to bacterial impairment.","authors":"Dongyang Ye, Jing Sun, Ran Jiang, Jiashen Chang, Yiming Liu, Xiangzheng Wu, Luqi Li, Yihan Luo, Juan Wang, Kangkang Guo, Zengqi Yang","doi":"10.3389/fmicb.2024.1514825","DOIUrl":"10.3389/fmicb.2024.1514825","url":null,"abstract":"<p><p>Understanding the impact of antibiotics on bacterial metabolism is crucial for elucidating their mechanisms of action and developing more effective therapeutic strategies. β-lactam antibiotics, distinguished by their distinctive β-lactam ring structure, are widely used as antimicrobial agents. This study investigates the global metabolic alterations induced by three β-lactam antibiotics-meropenem (a carbapenem), ampicillin (a penicillin), and ceftazidime (a cephalosporin)-in <i>Escherichia coli</i>. Our comprehensive metabolic profiling revealed significant perturbations in bacterial metabolism, particularly in pathways such as glutathione metabolism, pantothenate and CoA biosynthesis, pyrimidine metabolism, and purine metabolism. Antibiotic treatment markedly increased reactive oxygen species levels, with meropenem reaching nearly 200 ± 7%, ampicillin at 174 ± 11%, and ceftazidime at 152 ± 7%. Additionally, β-lactam antibiotics elevated 8-OHdG levels to 4.73 ± 0.56-fold for meropenem, 2.49 ± 0.19-fold for ampicillin, and 3.19 ± 0.34-fold for ceftazidime; 8-OHG levels increased to 5.57 ± 0.72-fold for meropenem, 3.08 ± 0.31-fold for ampicillin, and 4.45 ± 0.66-fold for ceftazidime, indicating that oxidative stress enhances oxidative damage to bacterial DNA and RNA. Notably, we observed a selective upregulation of specific amino acids associated with cellular repair mechanisms, indicating a metabolic adaptation to counteract oxidative damage. These findings illustrate that β-lactam antibiotics induce a complex metabolic perturbations associated with ROS production, potentially compromising critical cellular components. This study enhances our understanding of the intricate relationship between antibiotic action and bacterial metabolism, providing valuable insights for developing effective strategies against antibiotic-resistant pathogens.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"15 ","pages":"1514825"},"PeriodicalIF":4.0,"publicationDate":"2024-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11659197/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142876636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mycobiome analysis of leaf, root, and soil of symptomatic oil palm trees (<i>Elaeis guineensis</i> Jacq.) affected by leaf spot disease.","authors":"Abiodun Abeeb Azeez, Daniel Ofeoritse Esiegbuya, Adebola Azeez Lateef, Fred O Asiegbu","doi":"10.3389/fmicb.2024.1422360","DOIUrl":"10.3389/fmicb.2024.1422360","url":null,"abstract":"<p><p>Recently, attention has been shifting toward the perspective of the existence of plants and microbes as a functioning ecological unit. However, studies highlighting the impacts of the microbial community on plant health are still limited. In this study, fungal community (mycobiome) of leaf, root, and soil of symptomatic leaf-spot diseased (SS) oil palm were compared against asymptomatic (AS) trees using ITS2 rRNA gene metabarcoding. A total of 3,435,417 high-quality sequences were obtained from 29 samples investigated. Out of the 14 phyla identified, Ascomycota and Basidiomycota were the most dominant accounting for 94.2 and 4.7% of the total counts in AS, and 75 and 21.2% in SS, respectively. <i>Neopestalotiopsis</i> is the most abundant genus for AS representing 8.0% of the identified amplicons compared to 2.0% in SS while <i>Peniophora</i> is the most abundant with 8.6% of the identified amplicons for SS compared to 0.1% in AS. The biomarker discovery algorithm LEfSe revealed different taxa signatures for the sample categories, particularly soil samples from asymptomatic trees, which were the most enriched. Network analysis revealed high modularity across all groups, except in root samples. Additionally, a large proportion of the identified keystone species consisted of rare taxa, suggesting potential role in ecosystem functions. Surprisingly both AS and SS leaf samples shared taxa previously associated with oil palm leaf spot disease. The significant abundance of <i>Trichoderma asperellum</i> in the asymptomatic root samples could be further explored as a potential biocontrol agent against oil palm disease.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"15 ","pages":"1422360"},"PeriodicalIF":4.0,"publicationDate":"2024-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11659247/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142876590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Global research trajectories in gut microbiota and functional constipation: a bibliometric and visualization study.","authors":"Shun Seng Ong, Lianjie Xu, Ching Wei Ang, Xiaoyue Deng, Hai Lu, Tianshu Xu","doi":"10.3389/fmicb.2024.1513723","DOIUrl":"10.3389/fmicb.2024.1513723","url":null,"abstract":"<p><strong>Background: </strong>Functional constipation (FC) negatively impacts quality of life and is associated with gut microbiota (GM) imbalances. Despite the growing interest in this area, a thorough analysis of research trends is missing. This study uses bibliometric methods to assess the global research on GM's role in FC, pinpointing key topics, impactful studies, and prominent researchers to guide future research and identify gaps.</p><p><strong>Methods: </strong>In our study, we conducted a performance analysis and science mapping using bibliometric indicators such as publication trends, author and institutional contributions, productivity, impact, keyword analysis, and collaboration networks. We employed software tools like VOSviewer, Biblioshiny, CiteSpace, and SCImago Graphica to automate the assessment of metrics including country, institutional, and journal distribution, authorship, keyword frequency, and citation patterns.</p><p><strong>Results: </strong>From 2013 to 2024, annual publications on GM and FC rose from 29 to 252, with a slight decrease to 192 in 2024. Average citations per publication peaked at 11.12 in 2021, declining to 6.43 by 2024. China led in research output (37.8%), followed by the United States (14.4%) and Japan (7.5%). Bibliometric analysis identified key authors like CHEN W and ZHANG H, with 30 and 27 articles, respectively. Jiangnan University and Harvard University were top contributors, with 131 and 81 articles. Keywords analysis revealed \"constipation,\" \"gut microbiota,\" and \"probiotic\" as central themes, with a shift toward \"gut microbiota\" and \"intestinal flora\" in recent years. This study provides a comprehensive overview of the research landscape, highlighting leading authors, institutions, and evolving research priorities in the field.</p><p><strong>Conclusion: </strong>Our review synthesizes current GM and FC research, guiding future studies. It suggests exploring GM in various GI disorders, the impact of lifestyle and drugs on GM, advanced research techniques, and probiotics/prebiotics for FC. There's also a focus on therapies targeting GM's effect on the gut-brain axis, paving the way for improved FC management.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"15 ","pages":"1513723"},"PeriodicalIF":4.0,"publicationDate":"2024-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11659297/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142876471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Salt stress affects the bacterial communities in rhizosphere soil of rice.","authors":"Yujie Zhou, Zhizhou He, Qiuyun Lin, Yuehui Lin, Kaiyi Long, Zhenyu Xie, Wei Hu","doi":"10.3389/fmicb.2024.1505368","DOIUrl":"10.3389/fmicb.2024.1505368","url":null,"abstract":"<p><p>Salt is a primary factor limiting the utilization of saline lands in coastal beach areas, with rhizosphere microorganisms playing a crucial role in enhancing crop stress resistance and exhibiting high sensitivity to environmental changes. Rice (<i>Oryza sativa</i> L.) is the preferred crop for reclaiming salinized soils. This study determined the microbial communities in rhizosphere soil of rice under different salt stress treatments by high-throughput sequencing. We found that salt stress changed the bacterial community diversity, structure and function in rhizosphere soil of rice. Salt stress significantly reduced the richness and diversity of bacterial communities in rhizosphere soil of rice. The bacterial community was characterized by higher abundance of the phyla Chloroflexi, Proteobacteria and Actinobacteria; the relative abundances of Firmicutes, Acidobacteriota and Myxococcota were decreased, while Bacteroidota and Cyanobacteria were increased under salt stress. The functions of bacterial communities in rhizosphere soil of rice mainly include chemoheterotrophy, aerobic_chemoheterotrophy, phototrophy etc., chemoheterotrophy and aerobic_chemoheterotrophy were significantly higher NS3 (adding 3‰ NaCl solution to the base soil) treatment than NS6 (adding 6‰ NaCl solution to the base soil) treatment. These findings provide a theoretical foundation for the development of specialized salt-tolerant microbial agents for rice cultivation and offer a viable strategy for improving the soil environment of saline coastal lands through the application of beneficial microorganisms.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"15 ","pages":"1505368"},"PeriodicalIF":4.0,"publicationDate":"2024-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11659233/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142876601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dynamic microbial changes in exacerbation of chronic obstructive pulmonary disease.","authors":"Yong Jun Choi, Hye Jung Park, Chi Young Kim, Aeri Choi, Jae Hwa Cho, Min Kwang Byun","doi":"10.3389/fmicb.2024.1507090","DOIUrl":"10.3389/fmicb.2024.1507090","url":null,"abstract":"<p><strong>Background: </strong>Microbial profiles in patients with chronic obstructive pulmonary disease (COPD) provide insights for predicting, preventing, and treating exacerbations. This study aimed to analyze the impact of microbial diversity and spectrum on COPD exacerbation.</p><p><strong>Methods: </strong>From November 1, 2018, to May 31, 2023, we prospectively enrolled patients with stable disease (SD) and exacerbation of COPD (ECOPD). Sputum samples were collected for microbiome DNA sequencing, and amplicon sequence variants were analyzed.</p><p><strong>Results: </strong>We collected sputum samples from 38 patients: 17 samples from patients with SD and samples from patients with ECOPD at two time points-during exacerbation (AE-1: 21 samples) and again during stabilization after 2 weeks of treatment (AE-2: 17 samples). Alpha diversity indices, specifically observed feature count and Fisher's alpha index, were significantly higher in SD (133.0 [98.0-145.0]; 17.1 [12.7-19.6]) compared to AE-1 (88.0 [72.0-125.0], <i>p</i> = 0.025; 10.9 [8.5-16.1], <i>p</i> = 0.031). The SD showed significantly higher abundances of <i>Neisseria</i> (linear discriminant analysis [LDA] 4.996, adj.<i>p</i> = 0.021), <i>Fusobacterium</i> (LDA 3.688, adj.<i>p</i> = 0.047), and <i>Peptostreptococcus</i> (LDA 3.379, adj.<i>p</i> = 0.039) at the genus level compared to AE-1. At the species level, <i>N. perflava</i> (LDA 5.074, adj.<i>p</i> = 0.010) and <i>H. parainfluenzae</i> (LDA 4.467, adj. <i>p</i> = 0.011) were more abundant in SD. Hub genera in the microbial network included <i>Haemophilus</i>, <i>Granulicatella</i>, <i>Neisseria</i>, <i>Lactobacillus</i>, and <i>Butyrivibrio</i> in SD and <i>Streptococcus</i>, <i>Gemella</i>, <i>Actinomyces</i>, <i>Klebsiella</i>, and <i>Staphylococcus</i> in AE-1.</p><p><strong>Conclusion: </strong>COPD exacerbations are linked to changes in specific strains of normal flora. Maintaining microbial diversity and balance within the microbial network is critical for preventing and managing COPD exacerbations.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"15 ","pages":"1507090"},"PeriodicalIF":4.0,"publicationDate":"2024-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11659282/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142876374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physicochemical property and microbial community characteristics of the casing soil for cultivating <i>Oudemansiella raphanipes</i>.","authors":"Jinjia Liu, Zhongyu Qin, Jinqiang Wu, Jiao Su, Pengcheng Feng, Wenting Su","doi":"10.3389/fmicb.2024.1495168","DOIUrl":"https://doi.org/10.3389/fmicb.2024.1495168","url":null,"abstract":"<p><strong>Background: </strong>Casing soil is critical for the cultivation process of <i>Oudemansiella raphanipes</i> and promotes the formation of mushroom fruiting bodies. Therefore, reliable casing soil indicators are crucial for obtaining high yields of high-quality mushrooms.</p><p><strong>Methods: </strong>In this study, soil enzyme activity, physicochemical properties, and microorganisms at five cultivation stages [namely casing (A1), mycelial (A2), primordial (A3), fruiting (A4), and harvesting (A5)] of <i>O. raphanipes</i> cultivation were evaluated in casing soils.</p><p><strong>Results: </strong>The results indicated that sucrase and catalase activities were significantly increased with increasing cultivation time (<i>p</i> < 0.01), and the activities peaked [16.67 and 0.25 g/(g·h), respectively] at A4. Urease activity peaked [1.56 g/(g·h)] at A1, and it decreased gradually (<i>p</i> < 0.01). Polyphenol oxidase activity was significantly higher at A2 [0.95 g/(g·h)] than at the other stages and was significantly lower at A1 [0.06 g/(g·h)]. Soil pH peaked at A1 (8.20) and decreased gradually (<i>p</i> = 0.003). Soil total organic carbon content increased significantly with increasing cultivation time (<i>p</i> < 0.001) and was the highest at A5 (8.40 g/kg). The available phosphorus at A1 (0.40 g/kg) was significantly higher than those at the other stages (<i>p</i> = 0.004), and the available nitrogen at A1 (0.28 g/kg) and A3 (0.26 g/kg) was significantly higher than those at the other stages (<i>p</i> < 0.001). The number and diversity of bacteria and fungi in soil increased gradually, and nine bacterial and four fungal genera were identified.</p><p><strong>Conclusion: </strong>This study offers soil characteristic and microbial community data for <i>O. raphanipes</i> casing soil at different cultivation stages, which could facilitate sustainable cultivation of <i>O. raphanipes</i> and reduction of live contaminants.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"15 ","pages":"1495168"},"PeriodicalIF":4.0,"publicationDate":"2024-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11655482/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142863915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anaerobic <i>Faecalicatena</i> spp. degrade sulfoquinovose via a bifurcated 6-deoxy-6-sulfofructose transketolase/transaldolase pathway to both C₂- and C₃-sulfonate intermediates.","authors":"Sabrina Borusak, Karin Denger, Till Dorendorf, Corentin Fournier, Harry Lerner, Olga Mayans, Dieter Spiteller, David Schleheck","doi":"10.3389/fmicb.2024.1491101","DOIUrl":"10.3389/fmicb.2024.1491101","url":null,"abstract":"<p><p>Plant-produced sulfoquinovose (SQ, 6-deoxy-6-sulfoglucose) is one of the most abundant sulfur-containing compounds in nature and its bacterial degradation plays an important role in the biogeochemical sulfur and carbon cycles and in all habitats where SQ is produced and degraded, particularly in gut microbiomes. Here, we report the enrichment and characterization of a strictly anaerobic SQ-degrading bacterial consortium that produces the C₂-sulfonate isethionate (ISE) as the major product but also the C₃-sulfonate 2,3-dihydroxypropanesulfonate (DHPS), with concomitant production of acetate and hydrogen (H₂). In the second step, the ISE was degraded completely to hydrogen sulfide (H₂S) when an additional electron donor (external H₂) was supplied to the consortium. Through growth experiments, analytical chemistry, genomics, proteomics, and transcriptomics, we found evidence for a combination of the 6-deoxy-6-sulfofructose (SF) transketolase (sulfo-TK) and SF transaldolase (sulfo-TAL) pathways in a SQ-degrading <i>Faecalicatena</i>-phylotype (family Lachnospiraceae) of the consortium, and for the ISE-desulfonating glycyl-radical enzyme pathway, as described for <i>Bilophila wadsworthia,</i> in an <i>Anaerospora</i>-phylotype (Sporomusaceae). Furthermore, using total proteomics, a new gene cluster for a bifurcated SQ pathway was also detected in <i>Faecalicatena</i> sp. DSM22707, which grew with SQ in pure culture, producing mainly ISE, but also 3-sulfolacate (SL) 3-sulfolacaldehyde (SLA), acetate, butyrate, succinate, and formate, but not H₂. We then reproduced the growth of the consortium with SQ in a defined co-culture model consisting of <i>Faecalicatena</i> sp. DSM22707 and <i>Bilophila wadsworthia</i> 3.1.6. Our findings provide the first description of an additional sulfoglycolytic, bifurcated SQ pathway. Furthermore, we expand on the knowledge of sulfidogenic SQ degradation by strictly anaerobic co-cultures, comprising SQ-fermenting bacteria and cross-feeding of the sulfonate intermediate to H₂S-producing organisms, a process in gut microbiomes that is relevant for human health and disease.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"15 ","pages":"1491101"},"PeriodicalIF":4.0,"publicationDate":"2024-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11659671/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142876278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A rapid liquid chromatography-tandem mass spectrometry based method for the detection of Tet(X) resistance gene in <i>Enterobacteriaceae</i>.","authors":"Liyun Zhang, Jie Xie, Ziyu Qu, Duan Duan, Chujun Liu, Di Zhang, Haiyang Jiang, Xinhua Dai, You Jiang, Xiang Fang, Congming Wu","doi":"10.3389/fmicb.2024.1477740","DOIUrl":"10.3389/fmicb.2024.1477740","url":null,"abstract":"<p><p>There is a major public health threat posed by antibiotic resistance around the world. Tigecycline overcomes the resistance mechanisms of traditional tetracyclines and is often seen as the final resort in combating infections caused by bacteria resistant to multiple drugs. However, the introduction of new mobile tet(X) tetracycline destructases is leading to a notable rise in tigecycline resistance. Therefore, a rapid detection method is needed to monitor the spread of tigecycline resistance. In this study, a novel liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to detect tet(X) in bacterial isolates was developed. This method utilized the analysis by LC-MS/MS of metabolite ratios to determine the presence of tet(X). Bacterial suspensions were co-incubated with tigecycline for 1 h, where tet(X) destructase inactivated tigecycline, making a particular metabolite with a 16-Da change in mass. The characterized quantitative ion pairing of tigecycline in the ESI positive mode was observed at 586.1 → 569.1 m/z. The oxygenated tigecycline detection was established at 602.2 → 529.1 m/z. A model was established using 35 tet(X)-positive and 15 tet(X)-negative <i>Enterobacteriaceae</i> strains in this study to optimize the cutoff value. Applying the model to analyze 70 bacterial isolates, the sensitivity of the LC-MS/MS test was 98.9% compared to polymerase chain reaction (PCR), and specificity was 100%. This method is rapid and easy to operate, providing results within 1 h, making it more suitable for routine use in clinical microbiology laboratories.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":"15 ","pages":"1477740"},"PeriodicalIF":4.0,"publicationDate":"2024-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11659754/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142876069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}