Frontiers in Cellular and Infection Microbiology最新文献

{"title":"Evaluation of the Biosynex AMPLIQUICK^® Fecal Parasitology assays in a Belgian travel clinic.","authors":"Jasmine Coppens, Jean-Marc Schwob, Karin Van Looveren, Jolien Baeyens, Marjan Van Esbroeck","doi":"10.3389/fcimb.2026.1816447","DOIUrl":"10.3389/fcimb.2026.1816447","url":null,"abstract":"<p><strong>Introduction: </strong>Molecular diagnostics have improved infectious disease detection, yet their role in parasitology remains nuanced.</p><p><strong>Methods: </strong>We compared the performance of the AMPLIQUICK^®Fecal Parasitology assay with the conventional methods encompassing microscopy, antigen testing and in-house PCR testing, in a reference laboratory for parasitology in a travel clinic. The detection of protozoa, helminths, and microsporidia was evaluated on 107 stool samples from 99 patients with suspected gastrointestinal parasitic infections, of which 40 specimens were tested retrospectively and 67 were collected prospectively.</p><p><strong>Results: </strong>AMPLIQUICK^® demonstrated high accuracy for protozoa and microsporidia (94.6%, 35/37) detecting all <i>Entamoeba histolytica</i>, <i>Entamoeba dispar</i>, most <i>Giardia duodenalis</i> and <i>Cryptosporidium</i> spp., and all <i>Enterocytozoon bieneusi</i> and <i>Encephalitozoon</i> spp. The assay detected more <i>Dientamoeba fragilis</i> (24 vs. 12) and <i>Blastocystis</i> sp. (61 vs. 44) than the conventional method but the correctness of these results could not be established. AMPLIQUICK^® reliably detected most clinically relevant helminths, including <i>Ascaris lumbricoides</i>, <i>Strongyloides</i> spp., <i>Hymenolepis nana</i>, <i>Taenia</i> spp., and <i>Enterobius vermicularis.</i> Hookworms, <i>Trichuris trichiura</i>, <i>Schistosoma mansoni</i>, <i>Heterophyidae/Opisthorchidae</i> and <i>Trichostrongylus</i> spp. were occasionally missed.</p><p><strong>Discussion: </strong>AMPLIQUICK^® demonstrated good performance compared to the conventional methods and has the potential to improve parasitology diagnostics, particularly in laboratories with limited expertise. Clinicians should interpret findings in the patient's clinical context.</p>","PeriodicalId":12458,"journal":{"name":"Frontiers in Cellular and Infection Microbiology","volume":"16 ","pages":"1816447"},"PeriodicalIF":5.5,"publicationDate":"2026-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13683222/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149237619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diagnostic utility of the modified inflammatory burden index and PCT/LYM ratio for sepsis stratification and early prediction of septic shock.","authors":"Zhen-Zhen Ding, Qian Li, Chun-Yan Ma, Hang Li","doi":"10.3389/fcimb.2026.1845721","DOIUrl":"10.3389/fcimb.2026.1845721","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to investigate the diagnostic performance of a modified inflammatory burden index (mIBI) and conventional biomarkers in sepsis and septic shock and assess the clinical utility of their combined application with the PCT/LYM ratio.</p><p><strong>Methods: </strong>A retrospective analysis included 45 patients with sepsis, 77 with septic shock, and 54 with non-septic infectious inflammation (control). Levels of procalcitonin (PCT), C-reactive protein (CRP), interleukin-6 (IL-6), neutrophil count (NEU), and lymphocyte count (LYM) were measured. Composite indices including mIBI, PCT/LYM, CRP/LYM, and NLR were calculated. Multivariable logistic regression evaluated the independent diagnostic value of mIBI and PCT/LYM after adjusting for confounders.</p><p><strong>Results: </strong>Inflammatory markers and composite indices increased progressively from control to sepsis to septic shock, whereas LYM decreased (all <i>P</i> < 0.05). For sepsis diagnosis, PCT/LYM showed strong diagnostic performance (AUC = 0.851, 95% CI: 0.775-0.928; sensitivity 80.00%, specificity 81.48% at cutoff 0.91), followed by mIBI (AUC = 0.806, specificity 88.89%). For septic shock, PCT/LYM (AUC = 0.880) and mIBI (AUC = 0.877) were comparable to PCT and IL-6 (all AUC = 0.879). Multivariable analysis revealed that after adjustment for age, sex, qSOFA, diabetes, and renal insufficiency, both log₁₀(mIBI) (OR = 2.771, P < 0.001) and PCT/LYM (OR = 1.045, P = 0.002) were independent predictors for sepsis. For septic shock, log₁₀(mIBI) retained independent predictive capacity (OR = 1.399, P = 0.030), whereas PCT/LYM showed a borderline association (OR = 1.004, P = 0.053).</p><p><strong>Conclusion: </strong>The composite framework integrating PCT/LYM and mIBI addresses limitations of single biomarkers. PCT/LYM demonstrated independent predictive value, with a numerically higher AUC than PCT alone for both sepsis and septic shock, although the direct DeLong comparison did not reach statistical significance, supporting its potential utility as part of a composite screening approach. Modified IBI showed strong independent diagnostic value for sepsis and high univariate discriminative ability for septic shock. These routinely available indicators offer timely, cost-effective advantages for early sepsis identification and risk stratification.</p>","PeriodicalId":12458,"journal":{"name":"Frontiers in Cellular and Infection Microbiology","volume":"16 ","pages":"1845721"},"PeriodicalIF":5.5,"publicationDate":"2026-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13683176/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149237605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Glycogen synthase AfGsy2 regulates mycelial growth, stress response, predatory activity, and chlamydospore development in the nematode-trapping fungus <i>Arthrobotrys flagrans</i>.","authors":"Qianfei Shi, Yu Zhang, Minghe Mo, Guohong Li","doi":"10.3389/fcimb.2026.1891167","DOIUrl":"10.3389/fcimb.2026.1891167","url":null,"abstract":"<p><strong>Introduction: </strong>Glycogen is a universal carbon and energy reserve that plays crucial roles in fungal stress adaptation, cellular homeostasis, and predatory activity. However, the role of glycogen metabolism in nematode-trapping fungi has largely remained unexplored. Here, we performed a systematic functional characterization of <i>AfGsy2</i>, the glycogen synthase-encoding gene in the biocontrol fungus <i>Arthrobotrys flagrans</i>.</p><p><strong>Methods: </strong>Using a CRISPR-Cas9-mediated deletion mutant, together with overexpression and complementation strains, we investigated the involvement of AfGsy2 in mycelial growth, stress tolerance, trap formation, nematicidal activity against <i>Caenorhabditis elegans</i>, chlamydospore production and germination, and glycogen accumulation. Subcellular localization was examined using an AfGsy2-GFP fusion protein.</p><p><strong>Results: </strong>Deletion of <i>AfGsy2</i> reduced hyphal growth on both nutrient-rich and nutrient-poor media but enhanced tolerance to osmotic, salt, and oxidative stresses, suggesting that blocked glycogen synthesis may trigger metabolic rerouting toward alternative stress-protective compounds. Notably, the <i>ΔAfGsy2</i> mutants exhibited reduced trap production and nematicidal activity against <i>C. elegans</i>, whereas overexpression of <i>AfGsy2</i> accelerated these processes, suggesting a link between glycogen metabolism and predatory activity. Moreover, AfGsy2 positively regulated chlamydospore production, glycogen accumulation, and chlamydospore germination. AfGsy2-GFP predominantly localized to the vacuoles of chlamydospores, and complementation restored the mutant phenotypes to wild-type levels.</p><p><strong>Discussion: </strong>Collectively, these findings uncover a link between glycogen metabolism and growth, stress resilience, sporulation, and predatory activity in <i>A. flagrans</i>, and establish a molecular foundation for the rational improvement of nematode-trapping fungi. Together, these results identify AfGsy2 as a pleiotropic regulator linking glycogen metabolism to biocontrol-related traits.</p>","PeriodicalId":12458,"journal":{"name":"Frontiers in Cellular and Infection Microbiology","volume":"16 ","pages":"1891167"},"PeriodicalIF":5.5,"publicationDate":"2026-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13683002/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149237549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: The <i>CYP4G15</i>-<i>SORD</i>-like-associated pathway promotes ovarian ZIKV infection and egg-associated viral RNA detection in <i>Aedes albopictus</i>.","authors":"Mei Chen, Yajing Zhang, Wenwei Zhong, Mei Hong, Jinbao Gu, Zetian Lai","doi":"10.3389/fcimb.2026.1990923","DOIUrl":"https://doi.org/10.3389/fcimb.2026.1990923","url":null,"abstract":"<p><p>[This corrects the article DOI: 10.3389/fcimb.2026.1910501.].</p>","PeriodicalId":12458,"journal":{"name":"Frontiers in Cellular and Infection Microbiology","volume":"16 ","pages":"1990923"},"PeriodicalIF":5.5,"publicationDate":"2026-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13684789/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149237529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Periodontal diseases and coronary artery disease: epidemiological links, pathogenic mechanisms and prospects for comorbidity integrated management.","authors":"Chengkui Xu, Tong Yu, Lulu Zhang","doi":"10.3389/fcimb.2026.1946319","DOIUrl":"https://doi.org/10.3389/fcimb.2026.1946319","url":null,"abstract":"<p><p>Periodontal disease (PD) and coronary artery disease (CAD) are two highly prevalent chronic systemic disorders with overlapping population risk profiles. Accumulating observational, cellular, and genetic studies have suggested potential positive correlations between PD severity, periodontal pathogen load, and CAD incidence, coronary lesion severity, and major adverse cardiac events, whereas Mendelian randomization analyses may not support a direct causal link between the two illnesses. Several synergistic pathogenic pathways may mediate their association: periodontal pathogens may translocate into peripheral circulation to colonize atherosclerotic plaques and exert potential direct vascular toxicity; sustained systemic low-grade inflammation may disrupt endothelial homeostasis; molecular mimicry may induce cross-autoimmune vascular impairment; shared susceptibility loci and conventional cardiovascular risk factors may jointly amplify vascular damage. Standard non-surgical periodontal therapy may reduce systemic inflammatory burden and improve endothelial function, and clinical and microbial periodontal markers may serve as non-invasive biomarkers for CAD risk stratification. This review synthesizes epidemiological evidence and multilayered pathogenic mechanisms and proposes bidirectional collaborative interventions and multidisciplinary cardiostomatological management frameworks for comorbid patients. Further large-scale randomized controlled trials and multi-ethnic genetic analyses are still required to clarify causal inference and potential long-term cardiovascular benefits of periodontal intervention.</p>","PeriodicalId":12458,"journal":{"name":"Frontiers in Cellular and Infection Microbiology","volume":"16 ","pages":"1946319"},"PeriodicalIF":5.5,"publicationDate":"2026-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13680830/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149237521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Bacterial DNA involvement in carcinogenesis.","authors":"Han Shuwen","doi":"10.3389/fcimb.2026.2006085","DOIUrl":"https://doi.org/10.3389/fcimb.2026.2006085","url":null,"abstract":"<p><p>[This corrects the article DOI: 10.3389/fcimb.2022.996778.].</p>","PeriodicalId":12458,"journal":{"name":"Frontiers in Cellular and Infection Microbiology","volume":"16 ","pages":"2006085"},"PeriodicalIF":5.5,"publicationDate":"2026-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13682169/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149237553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An intravaginal ring for HIV pre-exposure prophylaxis: assessment of safety and pharmacokinetics in a randomized, placebo-controlled clinical trial.","authors":"Kathleen L Vincent, John A Moss, Mark A Marzinke, Richard B Pyles, Lauren Dawson, Trevelyn J Olive, Irina Butkyavichene, Amalia E Castonguay, John M Cortez, Mariana Remedios-Chan, Patricia Galvan, Sofia Rivera, Simon Webster, Manjula Gunawardana, Amy P Adler, Christina M Ramirez, Hans M L Spiegel, Craig W Hendrix, Peter A Anton, Marc M Baum","doi":"10.3389/fcimb.2026.1955105","DOIUrl":"10.3389/fcimb.2026.1955105","url":null,"abstract":"<p><strong>Introduction: </strong>In Eastern and Southern Africa, 63% of new HIV infections occurred in women and girls, with adolescent girls and young women representing a particularly vulnerable subpopulation. Effective vaginal products for HIV prevention are desirable because they are underrepresented in the currently approved portfolio and have attributes motivating product use. In this trial, we evaluated an innovative intravaginal ring (pod-IVR) as a platform for HIV pre-exposure prophylaxis (PrEP).</p><p><strong>Objective: </strong>To measure the safety and multicompartment pharmacokinetics (PKs) of a pod-IVR delivering tenofovir disoproxil fumarate (TDF) combined with emtricitabine (FTC) in healthy women over 28 days.</p><p><strong>Methods: </strong>The Phase 1 clinical trial consisted of a randomized order, controlled, double-blinded, crossover design. Ten women received either an active TDF-FTC pod-IVR or a placebo pod-IVR for 28 days, determined by 1:1 randomization, followed by product crossover for another 28 days. Safety was assessed in terms of adverse events related to product use, colposcopic examination, cervicovaginal epithelium thickness measurement by optical coherence tomography, and culture-independent assessment of the vaginal microbiome. Drug and/or drug metabolite concentrations were measured in cervicovaginal fluid (CVF), vaginal tissue, rectal tissue, rectal fluid, and plasma.</p><p><strong>Results: </strong>There was no evidence of a statistically significant difference in related adverse-event counts between study products. There also was no evidence suggesting the study product altered the vaginal microbiome systematically or reproducibly. Systemic plasma exposure to tenofovir (TFV) and FTC was low, consistent with topical delivery. High median CVF concentrations of TFV (38.9 ng mg^-1; 135×10³ fmol mg^-1) and FTC (239 ng mg^-1; 966×10³ fmol mg^-1) were measured. Median vaginal tissue concentrations of the anabolites responsible for inhibition of HIV reverse transcriptase exceeded our target concentrations: TFV diphosphate 0.651×10³ fmol mg^-1 and FTC triphosphate 0.180×10³ fmol mg^-1. Drug concentrations in rectal tissue samples generally were below the analytical lower limits of quantification, but were quantifiable in rectal fluids, 130 (TFV) and 300 (FTC) times lower than in CVF.</p><p><strong>Conclusions: </strong>The TDF-FTC pod-IVRs were safe and well-tolerated over the 28 days of consecutive use. The PK data met the target benchmarks associated with protection from vaginal HIV infection.</p><p><strong>Clinical trial registration: </strong>https://clinicaltrials.gov/study/NCT03255915, identifier NCT03255915.</p>","PeriodicalId":12458,"journal":{"name":"Frontiers in Cellular and Infection Microbiology","volume":"16 ","pages":"1955105"},"PeriodicalIF":5.5,"publicationDate":"2026-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13680163/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149230998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microplastics and nanoplastics as potential ecological oncogenic co-drivers: rewiring host-microbe interactions in cancer.","authors":"Richard Ponce-Cusi, Claudia Barletta-Carrillo, Nesstor Pilco-Ferreto","doi":"10.3389/fcimb.2026.1923626","DOIUrl":"https://doi.org/10.3389/fcimb.2026.1923626","url":null,"abstract":"<p><p>Microplastics and nanoplastics (MPs/NPs) are increasingly recognized as biologically active interfaces that may reshape host-microbe interactions in carcinogenesis. This review proposes a hypothesis-generating mechanistic framework in which MPs/NPs may influence cancer-related processes through the integration of environmental exposure, epithelial barrier disruption, immune dysregulation, and tumor microenvironment remodeling. Although applicable to multiple epithelial interfaces, the framework primarily focuses on the intestinal mucosa, where the most substantial evidence exists. Through plastisphere formation, MPs/NPs adsorb pollutants, virulence factors, and microbial communities, potentially facilitating pathogen persistence, horizontal gene transfer, and antibiotic resistance enrichment. Upon host exposure, MPs/NPs have been associated with compromised epithelial integrity, enhanced microbial translocation, and activation of inflammatory pathways involving pattern recognition receptors, NF-κB, and the NLRP3 inflammasome. At the microbiome level, MPs/NPs may promote dysbiosis, alter microbial metabolism, and favor pro-inflammatory and genotoxic metabolites that could reinforce oncogenic signaling. These processes may converge to sustain chronic inflammation, immune evasion, extracellular matrix remodeling, angiogenesis, and tumor-permissive niches. While experimental and biomarker-based evidence support this axis, direct human data remain limited. Collectively, this review provides a conceptual basis for understanding environmentally associated carcinogenesis through MPs/NPs as ecological mediators bridging the environmental exposures, microbiome, and cancer biology.</p>","PeriodicalId":12458,"journal":{"name":"Frontiers in Cellular and Infection Microbiology","volume":"16 ","pages":"1923626"},"PeriodicalIF":5.5,"publicationDate":"2026-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13680829/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149237576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrating Mendelian randomization with single-cell and spatial transcriptomics to investigate gut microbiota-associated genetic signals and molecular characteristics in prostate cancer.","authors":"Chenjie Qiu, Huilin Zhang, Zongmin Du, Yuan Gao, Liangliang Dai","doi":"10.3389/fcimb.2026.1899354","DOIUrl":"10.3389/fcimb.2026.1899354","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Background: &lt;/strong&gt;Prostate cancer (PRAD) represents one of the most prevalent malignancies among men globally. Accumulating evidence implicates gut microbiota dysbiosis in the initiation and progression of prostate cancer; however, the causal relationship and underlying molecular mechanisms remain incompletely elucidated. This study integrated Mendelian randomization (MR), bioinformatics, single-cell RNA sequencing, and spatial transcriptomics to systematically investigate the genetic associations between gut microbiota and prostate cancer risk and characterize the expression patterns of microbiota-associated candidate genes within the tumor microenvironment.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;Two-sample MR analyses were performed using summary statistics from the OpenGWAS database to evaluate causal associations between gut microbiota and PRAD risk, with sensitivity analyses conducted to ensure robustness. Significant SNPs were functionally annotated and mapped to candidate genes using g:SNPense, and differentially expressed associated genes (DEAGs) were identified through integration with GEO datasets. Subsequent analyses encompassed immune infiltration profiling, machine learning model construction, and nomogram validation. Single-cell RNA sequencing and spatial transcriptomics were employed to delineate cell type-specific expression and spatial distribution patterns of signature genes. Candidate gene expression alterations in prostate cancer cells were experimentally validated by quantitative real-time PCR (qRT-PCR).&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;MR analyses identified significant positive causal associations between PRAD risk and Brevibacillaceae, Brevibacillales, CAG-110, and Pararhizobium, whereas Lawsonibacter sp000492175 exhibited a significant inverse association. A total of 21 differentially expressed genes were identified, with RASSF3, CROCC, UBE4B, SMOC2, and ACACA emerging as key signature genes. Immune infiltration analyses revealed a significant positive correlation between FRMD3 and regulatory T cells (Tregs), and a negative correlation between ACACA and neutrophil infiltration. The random forest classifier demonstrated optimal predictive performance, achieving an area under the curve (AUC) of 0.853 in the validation cohort. Single-cell and spatial transcriptomic analyses revealed cell-type-associated expression patterns of ACACA, SMOC2, and UBE4B across different cellular populations. These findings suggest potential associations between signature genes and specific cellular contexts, which require further experimental validation. qRT-PCR validated differential expression patterns of these candidate genes in prostate cancer cells.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Conclusion: &lt;/strong&gt;This study provides genetic causal inference evidence supporting a close association between gut microbiota and prostate cancer risk, and identifies multiple key signature genes implicated in immune microenvironment regulation and tumor progression. Integ","PeriodicalId":12458,"journal":{"name":"Frontiers in Cellular and Infection Microbiology","volume":"16 ","pages":"1899354"},"PeriodicalIF":5.5,"publicationDate":"2026-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13680237/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149231010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prospects of pathogen whole-genome sequencing in outbreak tracing and antimicrobial resistance surveillance of spinal infections.","authors":"Xingsheng Yu, Zhen Jia, Jianhua Li, Zhaohu Mao, Zhengqi Chang","doi":"10.3389/fcimb.2026.1977484","DOIUrl":"https://doi.org/10.3389/fcimb.2026.1977484","url":null,"abstract":"<p><p>Spinal infections are a serious category of deep-seated infectious diseases that pose significant threats to human health. They are caused by a broad and complex spectrum of pathogens, including bacteria, fungi, and rare opportunistic organisms. Traditional diagnostic methods often face limitations in timeliness, accuracy, and source-tracing capability. Whole-genome sequencing (WGS), with its high resolution and throughput, provides comprehensive genetic information from pathogens, opening new avenues for precise diagnosis, outbreak tracing, and antimicrobial resistance monitoring and genomic epidemiology research in spinal infections., and enabling reliable identification of rare and fastidious pathogens that are difficult to characterize by conventional assays. This review systematically summarizes current applications of WGS in pathogen identification, clonal transmission inference, resistance gene and virulence factor analysis, and outbreak investigation. It further discusses the technical advantages, application challenges, and future development directions of WGS in outbreak tracing and resistance surveillance. Ultimately, this review aims to offer novel insights and a theoretical foundation for the precise prevention, control, and clinical management of spinal infections.</p>","PeriodicalId":12458,"journal":{"name":"Frontiers in Cellular and Infection Microbiology","volume":"16 ","pages":"1977484"},"PeriodicalIF":5.5,"publicationDate":"2026-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13681210/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149237578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}