Frontiers in Cellular and Infection Microbiology最新文献

{"title":"In-depth characterization of <i>Klebsiella pneumoniae</i> carbapenemase (KPC)-encoding plasmids points at transposon-related transmission of resistance genes.","authors":"Vincent F van Almsick, Annika Sobkowiak, Natalie Scherff, Franziska Schuler, Johannes Benedict Oehm, Christian Böing, Alexander Mellmann, Vera Schwierzeck","doi":"10.3389/fcimb.2025.1542828","DOIUrl":"10.3389/fcimb.2025.1542828","url":null,"abstract":"<p><p>Antimicrobial resistance (AMR) is a growing threat in healthcare systems, particularly in the management of infections in critically ill patients. This study highlights how to identify clusters and putative sharing of mobile genetic elements, such as transposons, in the hospital setting using long-read whole genome sequencing (lrWGS). The approach described here can be employed to investigate the transmission dynamics of KPC-3-positive <i>Klebsiella pneumoniae</i> at multiple levels, from the entire isolate down to individual plasmids and transposons. Here, a <i>bla</i> _KPC-3 harboring transposon cluster was identified by using a Mash-based distance calculation for plasmids. This approach was used to investigate a local accumulation of KPC-3-positive <i>Klebsiella pneumoniae</i> on surgical and infectious disease wards of a tertiary care center in Germany over a time of six months. In total, seven patients were affected. Core genome multi-locus sequence typing analysis (cgMLST) identified two distinct genetic clusters: a sequence type (ST) 307 cluster (n = 5) and a ST101 cluster (n = 2). All isolates carried a <i>bla</i> _KPC-3 carbapenemase. Further Mash distance-based plasmid analysis was not consistent with plasmid transfer due to genetic heterogeneity, but identified a transposon cluster across all isolates. Infection control evaluation of patient movements within their hospital admission supports a possible clonal transmission. Subsequent infection control measures, including point prevalence screening and enhanced contact precautions, successfully contained further transmissions. The study illustrates the value of in-depth plasmid analysis in understanding the transmission dynamics and epidemiology of AMR, particularly in hospital environments.</p>","PeriodicalId":12458,"journal":{"name":"Frontiers in Cellular and Infection Microbiology","volume":"15 ","pages":"1542828"},"PeriodicalIF":4.6,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11948313/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143729614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of intestinal homeostasis in sevoflurane-induced myelin development and cognitive impairment in neonatal mice.","authors":"Chang Liu, Jinjie Li, Ruizhu Liu, Guoqing Zhao","doi":"10.3389/fcimb.2025.1541757","DOIUrl":"10.3389/fcimb.2025.1541757","url":null,"abstract":"<p><strong>Background: </strong>Inhalational anesthetic sevoflurane is commonly used in pediatric anesthesia. Multiple exposures to sevoflurane in early postnatal life have been associated with long-term abnormalities in myelin development and cognitive and memory impairments, although the underlying mechanisms remain incompletely elucidated. Disruption of gut microbiota is recognized as an important contributor to neurological diseases. Here, we explore the potential mechanisms underlying the abnormal myelin development induced by multiple sevoflurane exposures in neonatal rats by analyzing gut homeostasis.</p><p><strong>Methods: </strong>Six-day-old (P6) C57BL/6 mice were exposed to 3% sevoflurane for 2 hours per day for three consecutive days. Mice exposed to a mixture of 60% nitrogen and oxygen under the same conditions and duration served as controls. Behavioral tests were conducted between P32 and P42. At P9 (24 hours after the last sevoflurane exposure) and P42 (after the completion of behavioral tests), intestinal and brain examinations were performed to investigate the effects of sevoflurane exposure during the lactation and adolescent periods on gut homeostasis and myelin development in mice. Subsequently, the ameliorative effects of butyrate supplementation on sevoflurane-induced abnormalities in myelin development and cognitive and memory impairments were observed.</p><p><strong>Results: </strong>After repeated exposure to sevoflurane, neonatal mice developed persistent gut microbiota imbalance accompanied by a decrease in short-chain fatty acids. Short-term intestinal inflammation emerged, with damage to the mucus layer and barrier function. In the hippocampus and prefrontal cortex, the expression of genes and transcription factors related to oligodendrocyte differentiation and myelin development was significantly affected, and these changes persisted even after the exposure ended. There was a reduction in proteins associated with oligodendrocytes and myelin formation, which had a certain impact on memory and cognitive behavior. This study also explored the potential connections between microbiota, metabolism, the gut, the brain, and behavior. Timely supplementation with butyrate could effectively reverse these changes, indicating that gut homeostasis is crucial for brain neurodevelopment.</p><p><strong>Conclusion: </strong>Multiple exposures to sevoflurane in neonatal mice disrupt gut homeostasis and affect oligodendrocyte differentiation and myelin development in the hippocampus and prefrontal cortex, inducing cognitive and memory impairments. Supplementation with butyrate can alleviate these changes.</p>","PeriodicalId":12458,"journal":{"name":"Frontiers in Cellular and Infection Microbiology","volume":"15 ","pages":"1541757"},"PeriodicalIF":4.6,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11936920/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143718470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tick HRF-dependent ferroptosis pathway to promote tick acquisition of <i>Babesia microti</i>.","authors":"Songqin Chen, Shanming Hu, Yongzhi Zhou, Jie Cao, Houshuang Zhang, Yanan Wang, Jinlin Zhou","doi":"10.3389/fcimb.2025.1560152","DOIUrl":"10.3389/fcimb.2025.1560152","url":null,"abstract":"<p><p><i>B. microti</i> is a tick-transmitted zoonotic erythrocytic intracellular parasite. Ferroptosis is an iron-dependent form of programmed cell death that affects pathogen replication in the host. Currently, there is limited research concerning the effect of tick ferroptosis on <i>Babesia</i> infection and the underlying mechanism of action. The present study used a <i>B. microti</i> -mouse- <i>Haemaphysalis longicornis</i> infection model in which nymphs fed on the blood of <i>B. microti</i>-infected mice. The midgut divalent iron (<i>p<0.01</i>) and reactive oxygen species (ROS) (<i>p<0.05</i>) levels were significantly elevated in infected ticks, and transmission electron microscopy (TEM) showed that mitochondrial ridges were absent or decreased in size. Downregulation of ferritin 1 and glutathione peroxidase 4 (GPX4) in ticks infected with <i>B. microti</i> suggests that these changes promote ferroptosis. <i>In vivo</i> studies demonstrated that the ferroptosis promoter Erastin increased <i>B. microti</i> load (<i>p<0.05</i>), while the inhibitor Ferrostatin-1 effectively decreased load (<i>p<0.01</i>). Tick histamine-releasing factor (HRF), a protein related to the antioxidant system, was downregulated in infected nymphs compared with uninfected nymphs (<i>p<0.05</i>), and interference with HRF promoted tick acquisition of <i>B. microti</i> (<i>p<0.001</i>). Transcriptomic analyses showed that HRF interference promotes tick ferroptosis by downregulating ferritin 1 and GPX4. Meanwhile, interference with tick HRF molecules showed increased divalent iron and ROS and decreased mitochondrial ridges compared with controls. These findings highlight the critical role of tick HRF molecules in regulating ferroptosis and acquisition of <i>B. microti</i>, thereby providing important insights for a deeper understanding of the tick-<i>Babesia</i> interaction.</p>","PeriodicalId":12458,"journal":{"name":"Frontiers in Cellular and Infection Microbiology","volume":"15 ","pages":"1560152"},"PeriodicalIF":4.6,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11936993/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143718471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum: Clinical characteristics and the role of IL-6 in acute-on-chronic liver failure patients with or without COVID-19: a multicenter paired cohort study.","authors":"Ruoyu Yao, Guofen Xu, Xiujuan Fu, Wenrui Zhang, Han Wang, Yu Chen, Jia Yao","doi":"10.3389/fcimb.2025.1584105","DOIUrl":"https://doi.org/10.3389/fcimb.2025.1584105","url":null,"abstract":"<p><p>[This corrects the article DOI: 10.3389/fcimb.2024.1471974.].</p>","PeriodicalId":12458,"journal":{"name":"Frontiers in Cellular and Infection Microbiology","volume":"15 ","pages":"1584105"},"PeriodicalIF":4.6,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11937595/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143718468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Predicting the immunological nonresponse to antiretroviral therapy in people living with HIV: a machine learning-based multicenter large-scale study.","authors":"Suling Chen, Lixia Zhang, Jingchun Mao, Zhe Qian, Yuanhui Jiang, Xinrui Gao, Mingzhu Tao, Guangyu Liang, Jie Peng, Shaohang Cai","doi":"10.3389/fcimb.2025.1466655","DOIUrl":"10.3389/fcimb.2025.1466655","url":null,"abstract":"<p><strong>Background: </strong>Although highly active antiretroviral therapy (HAART) has greatly enhanced the prognosis for people living with HIV (PLWH), some individuals fail to achieve adequate immune reconstitution, known as immunological nonresponse (INR), which is linked to poor prognosis and higher mortality. However, the early prediction and intervention of INR remains challenging in South China.</p><p><strong>Methods: </strong>This study included 1,577 PLWH who underwent at least two years of HAART and clinical follow-up between 2017 and 2022 at two major tertiary hospitals in South China. We utilized logistic multivariate regression to identify independent predictors of INR and employed restricted cubic splines (RCS) for nonlinear analysis. We also developed several machine-learning models, assessing their performance using internal and external datasets to generate receiver operating characteristic (ROC) curves, calibration curves, and decision curve analysis (DCA). The best-performing model was further interpreted using Shapley additive explanations (SHAP) values.</p><p><strong>Results: </strong>Independent predictors of INR included baseline, 6-month and 12-month CD4+ T cell counts, baseline hemoglobin, and 6-month hemoglobin levels. RCS analysis highlighted significant nonlinear relationships between baseline CD4+ T cells, 12-month CD4+ T cells and baseline hemoglobin with INR. The Random Forest model demonstrated superior predictive accuracy, with ROC areas of 0.866, 0.943, and 0.897 across the datasets. Calibration was robust, with Brier scores of 0.136, 0.102, and 0.126. SHAP values indicated that early CD4+T cell counts and CD4/CD8 ratio were crucial in predicting INR.</p><p><strong>Conclusions: </strong>This study introduces the random forest model to predict incomplete immune reconstitution in PLWH, which can significantly assist clinicians in the early prediction and intervention of INR among PLWH.</p>","PeriodicalId":12458,"journal":{"name":"Frontiers in Cellular and Infection Microbiology","volume":"15 ","pages":"1466655"},"PeriodicalIF":4.6,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11933112/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143708155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of FosA13, a novel fosfomycin glutathione transferase identified in a <i>Morganella morganii</i> isolate from poultry.","authors":"Runzhi Zhang, Yan Yu, Lulu Huang, Susu Chen, Ruxi Hu, Xiuxiu Wang, Dawei Huang, Chunhan Song, Junwan Lu, Qiyu Bao, Yunliang Hu, Pengfei Jiang, Wei Pan","doi":"10.3389/fcimb.2025.1534084","DOIUrl":"10.3389/fcimb.2025.1534084","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Background: &lt;/strong&gt;&lt;i&gt;M. morganii&lt;/i&gt; is a species of the genus &lt;i&gt;Morganella&lt;/i&gt; in the family &lt;i&gt;Enterobacteriaceae&lt;/i&gt;. This species primarily causes infections of postoperative wounds and the urinary tract. Some isolates of &lt;i&gt;M. morganii&lt;/i&gt; exhibit resistance to multiple antibiotics due to multidrug resistance traits, complicating clinical treatment; thus, there is a growing need to elucidate the resistance mechanisms of this pathogen.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;A total of 658 bacterial strains were isolated from anal fecal swabs from poultry and livestock and from the surrounding environment in Wenzhou, China, via plate streaking. The full genome sequences of the bacteria were obtained via next-generation sequencing platforms. The standard agar dilution method was employed to determine the minimum inhibitory concentrations (MICs) of various antimicrobial agents. The resistance gene (&lt;i&gt;fosA13&lt;/i&gt;) of the isolate was identified using the Comprehensive Antibiotic Resistance Database (CARD) and confirmed via molecular cloning. The FosA13 protein encoded by the novel resistance gene &lt;i&gt;fosA13&lt;/i&gt; was expressed with the vector pCold I, and its enzyme kinetics parameters were characterized. The genetic background and evolutionary process of the sequence of this novel resistance gene were analyzed by means of bioinformatics methods.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;In this study, we identified a new chromosomally encoded fosfomycin resistance gene, designated &lt;i&gt;fosA13&lt;/i&gt;, from the &lt;i&gt;M. morganii&lt;/i&gt; isolate DW0548, which was isolated from poultry on a farm in Wenzhou, China. Compared with the control strain (pUCP19/DH5α), the recombinant strain carrying &lt;i&gt;fosA13&lt;/i&gt; (pUCP19-&lt;i&gt;fosA13&lt;/i&gt;/DH5α) presented a fourfold increase in the MIC value for fosfomycin. The enzyme kinetics data of FosA13 revealed effective inactivation of fosfomycin, with a &lt;i&gt;k&lt;/i&gt; &lt;sub&gt;cat&lt;/sub&gt; &lt;i&gt;/K&lt;/i&gt; &lt;sub&gt;m&lt;/sub&gt; of (1.50 ± 0.02)×10&lt;sup&gt;4&lt;/sup&gt; M&lt;sup&gt;-1&lt;/sup&gt;·s&lt;sup&gt;-1&lt;/sup&gt;. Among functionally characterized resistance proteins, FosA13 presented the highest amino acid (aa) homology (55.6%) with FosA. FosA13 also contained essential functional residues of FosA proteins. The isolate &lt;i&gt;M. morganii&lt;/i&gt; DW0548 presented high MIC values (≥ 8 μg/mL) for 5 classes of antimicrobials, namely, aminoglycosides, β-lactams, quinolones, tetracycline, and chloramphenicol, but only two functionally characteristic antimicrobial resistance genes (ARGs) have been identified in the complete genome: a β-lactam resistance gene (&lt;i&gt;bla&lt;/i&gt; &lt;sub&gt;DHA-16&lt;/sub&gt;) and a phenol resistance gene (&lt;i&gt;catII&lt;/i&gt;). These findings indicate that in addition to the novel resistance gene identified in this work, other uncharacterized resistance mechanisms might exist in &lt;i&gt;M. morganii&lt;/i&gt; DW0548.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Conclusion: &lt;/strong&gt;A novel chromosomal fosfomycin resistance gene, &lt;i&gt;fosA13&lt;/i&gt;, was identified in an animal &lt;i&gt;M. morganii&lt;/i&gt; isolate, and its enzymatic parameters were characteri","PeriodicalId":12458,"journal":{"name":"Frontiers in Cellular and Infection Microbiology","volume":"15 ","pages":"1534084"},"PeriodicalIF":4.6,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11933065/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143709455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploration of the primary antibiofilm substance and mechanism employed by <i>Lactobacillus salivarius</i> ATCC 11741 to inhibit biofilm of <i>Streptococcus mutans</i>.","authors":"Nan Ma, Wei Yang, Bairu Chen, Meihua Bao, Yimin Li, Meng Wang, Xiaopeng Yang, Junyi Liu, Chengyue Wang, Lihong Qiu","doi":"10.3389/fcimb.2025.1535539","DOIUrl":"10.3389/fcimb.2025.1535539","url":null,"abstract":"<p><strong>Introduction: </strong><i>Lactobacillus salivarius</i> serves as a probiotic potentially capable of preventing dental caries both <i>in vitro</i> and <i>in vivo</i>. This study focused on understanding the key antibiofilm agents and the mechanisms of action of the <i>Lactobacilli</i> supernatant against <i>Streptococcus mutans</i>.</p><p><strong>Methods: </strong><i>Streptococcus mutans</i> biofilm was constructed and the cell-free supernatant of <i>Lactobacillus salivarius</i> was added. After the biofilm was collected, RNA-seq and qRT-PCR were then performed to get gene information. The influence of temperature, pH and other factors on the supernatant were measured and non-targeted metabolome analysis was performed to analyze the effective components.</p><p><strong>Results: </strong>The findings indicated that the supernatant derived from <i>Lactobacillus salivarius</i> could inhibit the biofilm formation of <i>Streptococcus</i> mutans at different times. Through transcriptome analysis, we discovered that the cell-free supernatant reduced biofilm formation, by suppressing phosphoenolpyruvate-dependent phosphotransferase systems along with two ATP-binding cassette transporters, rather than directly affecting the genes that code for glucosyltransferases; additionally, the supernatant was observed to diminish the expression of genes linked to two-component systems, polyketides/non-ribosomal peptides, acid stress response, quorum sensing, and exopolysaccharide formation. Non-targeted LC-MS/MS analysis was employed to discover a variety of potential active compounds present in the cellular filtrate of <i>Lactobacillus salivarius</i> that hinder the growth of S. mutans, including phenyllactic acid, sorbitol, and honokiol.</p><p><strong>Discussion: </strong>In summary, our findings support the evaluation of <i>Lactobacillus salivarius</i> as a promising oral probiotic aimed at hindering the formation of biofilms by cariogenic pathogens and the development of dental caries.</p>","PeriodicalId":12458,"journal":{"name":"Frontiers in Cellular and Infection Microbiology","volume":"15 ","pages":"1535539"},"PeriodicalIF":4.6,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11933110/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143709456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic epidemiology and public health implications of zoonotic monophasic <i>Salmonella</i> Typhimurium ST34.","authors":"Xiaolei Wu, Jiaxin Du, Xiao Zhou, Xianqi Peng, Chenghao Jia, Baikui Wang, Beibei Wu, Yan Li, Min Yue","doi":"10.3389/fcimb.2025.1490183","DOIUrl":"10.3389/fcimb.2025.1490183","url":null,"abstract":"<p><strong>Background: </strong>Monophasic <i>Salmonella</i> Typhimurium sequence type 34 (mSTM ST34) has emerged as a significant global health threat, but our understanding of its genomic epidemiology and potential public health implications in international and regional contexts remains limited. This study aims to fill this crucial gap by assessing the genomic epidemiology of multidrug resistance (MDR) mSTM ST34, as well as its clinical characteristics and virulence.</p><p><strong>Methods: </strong>To achieve the objectives of this study, we conducted a comprehensive genomic analysis of mSTM ST34 isolates. We obtained a global dataset comprising 13,844 strains from public databases, along with 339 strains from a regional surveillance collection in Zhejiang Province, China. This dataset aims to provide in-depth insights into antimicrobial resistance, mobile genetic elements, and pathogenicity. Additionally, we meticulously assessed the association between phenotypic profiles and clinical presentations.</p><p><strong>Results: </strong>Our findings revealed that the prevalence of mSTM ST34 has surpassed that of the previously dominant ST19. In addition, we observed an increase in the detection of the IncQ1 plasmid, which is responsible for disseminating MDR. The prevalence of mSTM ST34 carriage was exceptionally high among children (≤12 years old) and elderly individuals (≥65 years old), with 92.6% of the isolates exhibiting MDR, including resistance to frontline antimicrobials such as third-generation cephalosporins and ciprofloxacin. Additionally, the human mSTM ST34 strain demonstrates a remarkable capacity for biofilm formation, which increases its virulence in animal models and complicates therapeutic interventions.</p><p><strong>Conclusions: </strong>mSTM ST34 has surpassed the previously dominant ST19, and its ability to transmit across multi-species increases its potential for further human transmission. This study addresses critical gaps in our understanding of mSTM ST34 prevalence, highlighting the importance of whole genome sequencing in surveilling zoonotic pathogens.</p>","PeriodicalId":12458,"journal":{"name":"Frontiers in Cellular and Infection Microbiology","volume":"15 ","pages":"1490183"},"PeriodicalIF":4.6,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11933091/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143709458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The microbial metabolite trimethylamine N-oxide and the kidney diseases.","authors":"Jin-Qi Su, Xiang-Qi Wu, Qi Wang, Bo-Yang Xie, Cui-Yan Xiao, Hong-Yong Su, Ji-Xin Tang, Cui-Wei Yao","doi":"10.3389/fcimb.2025.1488264","DOIUrl":"10.3389/fcimb.2025.1488264","url":null,"abstract":"<p><p>Trimethylamine N-oxide (TMAO), a metabolite, is a co-metabolite produced by both gut microbiota and livers, originating from foods rich in choline or carnitine. Emerging evidence suggests that TMAO may play a role in the pathogenesis of various kidney diseases, including acute kidney injury and chronic kidney disease. Research has demonstrated that heightened levels of TMAO are correlated with a heightened likelihood of kidney disease advancement and cardiovascular incidents among individuals with chronic kidney disease. Furthermore, TMAO has been observed to stimulate inflammation, oxidative stress, and fibrosis in animal models of kidney disease. Mechanistically, TMAO may contribute to kidney disease pathogenesis by inhibiting autophagy, activating the NLRP3 inflammasome, and inducing mitochondrial dysfunction. Therefore, targeting TMAO may represent a promising therapeutic strategy for the treatment of kidney diseases. Future studies are needed to further investigate the role of TMAO in kidney disease pathogenesis and to develop TMAO-targeted therapies for the prevention and treatment of kidney diseases.</p>","PeriodicalId":12458,"journal":{"name":"Frontiers in Cellular and Infection Microbiology","volume":"15 ","pages":"1488264"},"PeriodicalIF":4.6,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11933022/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143708167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fungi from <i>Malus</i> in Qujing, China: two new species, three new records, and insights into potential host jumping and lifestyle switching.","authors":"Gui-Qing Zhang, Zhu-Mei Li, Xin-Lei Fan, Qi-Rui Li, Jaturong Kumla, Nakarin Suwannarach, Abdallah M Elgorban, Ihab M Moussa, Dong-Qin Dai, Nalin N Wijayawardene","doi":"10.3389/fcimb.2025.1517908","DOIUrl":"10.3389/fcimb.2025.1517908","url":null,"abstract":"<p><p>Apple trees [<i>Malus domestica</i> Borkh. (<i>Rosaceae</i>)] are one of the important temperate fruit crops in China. In comparison to other temperate fruits, such as grapes and pears, fungal studies (in Yunnan) associated with <i>M. domestica</i> are fewer in number. In the present study, we investigated fungi associated with <i>M. domestica</i> in Qujing City, Yunnan Province, China. Samples were collected from apple gardens in different locations. Single spore isolation was carried out to isolate saprobic fungi, while the surface sterilization method was carried out to isolate endophytic fungi. Molecular analyses were carried out to determine the phylogenetic placement of the new collections. Based on the combined methods of morphology and phylogeny, <i>Cytospora qujingensis</i> sp. nov. and <i>Hypoxylon malongense</i> sp. nov. are introduced as novel saprobic and endophytic taxa, respectively. Moreover, <i>Aureobasidium pullulans</i> and <i>Cytospora schulzeri</i> are reported as new geological records from southwestern China. <i>Allocryptovalsa castaneae</i> is reported on <i>M. domestica</i> in China for the first time. The checklist of fungi associated with <i>M. domestica</i> in China is presented.</p>","PeriodicalId":12458,"journal":{"name":"Frontiers in Cellular and Infection Microbiology","volume":"15 ","pages":"1517908"},"PeriodicalIF":4.6,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11933099/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143709457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}