Frontiers in Cellular and Infection Microbiology最新文献

{"title":"Investigating the <i>in-vitro</i> antimicrobial activities of sorghum [<i>Sorghum bicolor</i> (L.) Moench] phenolic extracts on liver abscess causing bacterial pathogens.","authors":"Harith M Salih, Raghavendra G Amachawadi, Qing Kang, Dmitriy Smolensky, Ramasamy Perumal, Sarah-Sexton Bowser, P V Vara Prasad, T G Nagaraja","doi":"10.3389/fcimb.2025.1568504","DOIUrl":"10.3389/fcimb.2025.1568504","url":null,"abstract":"<p><strong>Introduction: </strong>Liver abscesses that occur in finishing cattle fed high-grain, low-roughage diets, are of significant economic concern to the feedlot industry. The causative agents include both <i>Fusobacterium necrophorum</i> subspecies (<i>necrophorum</i> and <i>funduliforme</i>), <i>Trueperella pyogenes</i>, and <i>Salmonella enterica</i> serotype Lubbock. Tylosin, a macrolide antibiotic, is supplemented in the feed to reduce liver abscesses. Because of the concern with emergence of potential antimicrobial resistance, there is a need to find antibiotic alternatives. The aim of our study was to investigate the efficacy of phenolic compounds extracted from black and brown sumac sorghum extracts on liver abscess causing bacterial pathogens.</p><p><strong>Methods: </strong>Phenolic compounds were extracted by 75% aqueous acetone and total phenolic content was determined spectrophotometrically. Muller-Hinton broth (for <i>S. enterica</i> and <i>T. pyogenes</i>), and anaerobic Brain-Heart infusion broth (for <i>Fusobacterium</i>) with and without sorghum extracts (1 mg GAE/mL) were used. Growth was measured at 24 and 48 hours to determine bacterial concentration. Micro-broth dilution method was used to quantify growth inhibition.</p><p><strong>Results: </strong>Plant based phenolic compounds have the potential to be an antibiotic alternative to control liver abscesses. Sorghum [<i>Sorghum bicolor</i> (L.) Moench] grain phenolic compounds, have the potential to be one of these alternatives.</p><p><strong>Discussion: </strong>Our study demonstrated that the phenolic extracts of black and brown sumac sorghum exhibited antibacterial activities against the liver abscesses causing pathogens including both subspecies of <i>F. necrophorum</i> and <i>T. pyogenes</i> in a dose dependent manner, but not <i>S. enterica</i>. Sorghum phenolic compounds have the potential to be supplemented in the cattle feed to control liver abscesses.</p>","PeriodicalId":12458,"journal":{"name":"Frontiers in Cellular and Infection Microbiology","volume":"15 ","pages":"1568504"},"PeriodicalIF":4.6,"publicationDate":"2025-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12241046/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144607965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"NO: a key player in microbiome dynamics and cancer pathogenesis.","authors":"Seyedeh Kimia Jasemi, Hossein Faridafshar, Mohammed Namiq Amin, Mehregan Babamohamadi, Marjan Falahati, Roshanak Amirian, Zhila Izadi","doi":"10.3389/fcimb.2025.1532255","DOIUrl":"10.3389/fcimb.2025.1532255","url":null,"abstract":"<p><p>The human microbiome refers to the genomic content of microorganisms inhabiting the human body, including the lungs, oral cavity, intestinal tract, esophagus, and other areas. The human oral microbiota is a diverse and complex ecosystem that includes bacteria, microeukaryotes, archaea, and viruses. These communities have a highly structured biogeography resulting from the various microenvironments in the oral cavity, shaping local metabolic exchange. Dietary nitrate (NO₃ ^-) is an ion naturally present in vegetables, especially leafy greens. When consumed, it leads to the production of nitric oxide (NO). This bioactive molecule benefits bodily functions like host defense and neuronal communication and improves vascular and metabolic health. Dietary NO₃ ^- is reduced to NO via the nitrate-nitrite-NO pathway, facilitated by nitrate-reducing bacteria inside the oral cavity. NO has a leading role in different types of diseases, including cancer, cardiovascular disease, and diabetes. The bioavailability of NO is greatly enhanced by the activity of bacteria residing in the mouth, which reduces NO₃ ^-to NO₂ ^- and increases the concentration of circulating NO₂ ^-. NO is the key to causing different malignancies, including gastrointestinal cancers. NO can cause cell death by inducing DNA damage and anti-apoptotic signaling pathways. Low to moderate levels of NO derived from tumors can activate angiogenesis and promote an invasive phenotype, while high levels of NO may have an anti-tumor effect in protecting against cancer. In this review, we intend to discuss the human microbiome, dietary NO₃ ^-consumption, the vital role of NO in the human body, types of cancers, and treatments based on it.</p>","PeriodicalId":12458,"journal":{"name":"Frontiers in Cellular and Infection Microbiology","volume":"15 ","pages":"1532255"},"PeriodicalIF":4.6,"publicationDate":"2025-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12241020/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144607966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Periodontal disease and chronic kidney disease: mechanistic insights and novel therapeutic perspectives.","authors":"Shuxin Li, Hongliang Cao, Yueqiu Zhang, Fulin Wang, Gengchen Huang, Binbin Wang, Wei Wei, Gang Wang","doi":"10.3389/fcimb.2025.1611097","DOIUrl":"10.3389/fcimb.2025.1611097","url":null,"abstract":"<p><p>Periodontal disease (PD) is one of the most common chronic diseases of the oral cavity, and it usually refers to chronic inflammatory diseases caused by the infection of pathogenic microorganisms in the oral cavity. PD mainly affects the tissues around the teeth, causing inflammation of the gums and periodontium, destroying the alveolar bone, and even leading to tooth loosening. Many studies have shown that PD is not only limited to oral health but is also associated with diseases of multiple systems throughout the body. In recent years, more and more studies have focused on the interaction between PD and urologic diseases, especially chronic kidney disease (CKD). PD is also a typical oral disease frequently observed in patients with kidney disease. This paper reviews the potential link between PD and CKD and discusses the interaction's pathologic mechanisms and clinical implications. In addition, this review aims to identify possible pathogenic mechanisms and suggest potential ways to target PD to prevent and treat CKD. This would lead to better treatment options to delay the progression of CKD.</p>","PeriodicalId":12458,"journal":{"name":"Frontiers in Cellular and Infection Microbiology","volume":"15 ","pages":"1611097"},"PeriodicalIF":4.6,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12237910/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144599888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High-yield bioproduction of virus-free virus-like P4-EKORhE multi-lysin transducing particles as an antimicrobial gene therapeutic.","authors":"Robert Ramirez-Garcia, Antonia P Sagona, Jeremy J Barr, Alfonso Jaramillo","doi":"10.3389/fcimb.2025.1561443","DOIUrl":"10.3389/fcimb.2025.1561443","url":null,"abstract":"<p><p>A description of the construction of the bioengineered P4-EKORhE and a comprehensive method for producing very high yields (up to 10¹² particles per millilitre) enable the use of virus-like particles to transduce genetically encoded antimicrobials through a combination of synthetic biology and optimised upstream and downstream processing. The final product, a gene-delivered antimicrobial in the form of the multi-lysin cassette, is fully functional before and after packaging within P4-EKORhE particles. The antimicrobial activity of the multi-lysin cassette, characterised by its lysis proteins, was tested <i>in vivo</i> in both pure bacterial <i>Escherichia coli</i> cultures and a model of phage infection in co-culture with A549 immortalised human epithelial tissue cells. This work exemplifies several bioproduction methods and demonstrates how the virology of the P4 and P2 phages can be harnessed to establish a bioprocess for producing transducing particles at very high yields, avoiding contamination by the natural virus while maintaining the antimicrobial effectiveness of the final product.</p>","PeriodicalId":12458,"journal":{"name":"Frontiers in Cellular and Infection Microbiology","volume":"15 ","pages":"1561443"},"PeriodicalIF":4.6,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12239096/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144599887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aryl hydrocarbon receptor facilitates HSV-1 lytic infection by enhancing viral gene transcription and receptor expression.","authors":"Pu Huang, Xiaohong Pan, Hongli Chen, Mengyue Lei, Ying Ma, Xiaomei Guo, Jiaxin Xie, Jixiong Li, Jing Sun, Yunzhang Hu, Jiandong Shi","doi":"10.3389/fcimb.2025.1548038","DOIUrl":"10.3389/fcimb.2025.1548038","url":null,"abstract":"<p><p>Herpes simplex virus type 1 (HSV-1) is a major human pathogen with significant morbidity in neonates and immunocompromised individuals. However, antiviral drugs targeting HSV-1 are emerging with antiviral resistance, highlighting the need to identify new targets for future treatment. Once HSV-1 enters the host cells, it recruits host factors to facilitate viral life cycle. In this study, we showed that aryl hydrocarbon receptor (AhR), a ligand-activated nuclear receptor, is required for HSV-1 effective replication and offers an opportunity for pharmacological intervention. Our results showed that HSV-1 infection activates AhR signaling in an interferon-dependent manner. Pharmacological inhibition or knockout of AhR reduced the expression of viral proteins and infectious progeny, while increased AhR signaling promoted the expression of viral proteins and viral replication. Mechanistically, AhR formed a transcription complex with cyclin T1, VP16 and RNA Pol II in the nucleus, bound to viral gene promoters, and promoted their transcription. Additionally, AhR promoted viral replication partially by facilitating the expression of multiple viral receptors. Collectively, AhR is a proviral host factor for HSV-1, and thus may be used as a promising host-directed antiviral target.</p>","PeriodicalId":12458,"journal":{"name":"Frontiers in Cellular and Infection Microbiology","volume":"15 ","pages":"1548038"},"PeriodicalIF":4.6,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12238220/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144599884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and application of a curcumin-cinnamon essential oil nanoemulsion agent against mycobacteria.","authors":"Zhihui Lei, Yixuan Ren, Jinyao Wang, Haisu Shi, Hu Lin, Hong Chen, Lijun Bi, Yutang Wang, Hongtai Zhang","doi":"10.3389/fcimb.2025.1582416","DOIUrl":"10.3389/fcimb.2025.1582416","url":null,"abstract":"<p><p>With the increasing prevalence of drug-resistant <i>Mycobacterium tuberculosis</i> (<i>M. tuberculosis</i>), the development of novel anti-mycobacterial agents has become urgent. In this study, a curcumin-cinnamon essential oil (Cur-CEO) nanoemulsion was developed with optimized preparation parameters, including a 10% CEO volume fraction, 7.5 minutes of ultrasonic treatment, and 350 W ultrasound power, yielding a particle size of 101.14 nm. The nanoemulsion demonstrated high encapsulation efficiency (90.2%) and stability, with a stability coefficient of 0.984. Structural analysis revealed a dense network structure of the nanoemulsion and amorphous forms of Cur and CEO, enhanced by hydrogen bonding and electrostatic interactions, which improved solubility and bioavailability. The Cur-CEO nanoemulsion exhibited potent antimicrobial activity against mycobacteria, demonstrating MIC values of 2 µg/mL and 0.25 µg/mL against <i>Mycobacterium smegmatis</i> and <i>M. tuberculosis</i>, respectively, representing a fourfold reduction compared to the CEO solution alone, owing to its ability to induce substantial damage to mycobacterial cell membranes and consequently enhance nucleic acid and protein leakage. Furthermore, the aerosol form of the nanoemulsion effectively inhibited both surface and airborne mycobacteria, with no significant changes in structural properties post-atomization. Lung deposition studies indicated that 75.6% of aerosol particles of the nanoemulsion reached the alveolar region, suggesting its potential as an inhalation agent. Additionally, the Cur-CEO nanoemulsion exhibited negligible effects on macrophage viability, maintaining a survival rate exceeding 85% even at concentrations up to 1250 ng/mL. These findings indicate that the Cur-CEO nanoemulsion, formulated using natural ingredients, holds significant promise as a food-grade antibacterial agent for the prevention and control of mycobacterial infections.</p>","PeriodicalId":12458,"journal":{"name":"Frontiers in Cellular and Infection Microbiology","volume":"15 ","pages":"1582416"},"PeriodicalIF":4.6,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12238011/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144599885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of innate immune system in respiratory viral infection related asthma.","authors":"Xiao Wu, Feifei Huang, Wenbo Yao, Zheng Xue","doi":"10.3389/fcimb.2025.1604831","DOIUrl":"10.3389/fcimb.2025.1604831","url":null,"abstract":"<p><p>The association between viral infections and asthma has garnered significant attention in recent years. Accumulating evidence suggests that viral infections can trigger asthma exacerbations, while asthma may also influence the susceptibility to viral infections, thereby creating a cycle of worsening symptoms and recurrent asthma attacks. Given that asthma is predominantly driven by Type 2 immune responses and viral infections are typically associated with Type 1 immune responses, the innate immune cells and cytokines that participate in both conditions appear to be the critical bridge connecting these two processes. In particular, innate immune cells play a pivotal role in modulating the immune response at the interface of viral infections and asthma. In this review, we summarize the key innate immune cells and cytokines involved in viral infections and asthma, highlighting their immunoregulatory mechanisms. We aim to provide novel perspectives and potential therapeutic directions for the clinical management of recurrent asthma attacks induced by viral infections.</p>","PeriodicalId":12458,"journal":{"name":"Frontiers in Cellular and Infection Microbiology","volume":"15 ","pages":"1604831"},"PeriodicalIF":4.6,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12237907/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144599889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Editorial: The microbiome in surgery - friend or foe?","authors":"Ann-Kathrin Lederer, Sophia Chikhladze, Mohamed Tarek Badr","doi":"10.3389/fcimb.2025.1629822","DOIUrl":"https://doi.org/10.3389/fcimb.2025.1629822","url":null,"abstract":"","PeriodicalId":12458,"journal":{"name":"Frontiers in Cellular and Infection Microbiology","volume":"15 ","pages":"1629822"},"PeriodicalIF":4.6,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12238079/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144599886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>SIRT7</i> deletion inhibits <i>Glaesserella parasuis</i>-mediated inflammatory responses in porcine alveolar macrophages.","authors":"Hao Zheng, Baoxin Wang, Xia Dong, Junjing Wu, Liangyu Shi, Jing Zhang, Hongbo Chen, Ao Zhou","doi":"10.3389/fcimb.2025.1589199","DOIUrl":"10.3389/fcimb.2025.1589199","url":null,"abstract":"<p><p><i>Glaesserella parasuis</i> (GPS) infection causes severe inflammatory disorder, resulting in lung injury. <i>SIRT7</i> is an NAD⁺-dependent deacetylase known to regulate inflammatory responses, but its role in GPS infection remains unclear. Here we found that GPS infection increased <i>SIRT7</i> expression and induced inflammatory responses. Deficiency of <i>SIRT7</i> by CRISPR/Cas9 technology significantly inhibited GPS-induced cytopathic effects and inflammatory responses. In addition, RNA-seq analysis showed that differentially expressed genes(DEGs) induced by <i>SIRT7</i> deficiency were enriched in biological processes such as cell proliferation, actin cytoskeleton formation, lipid synthesis, protein kinase activation regulation, and GTPase activity regulation. Functional enrichment analysis further indicated the involvement of these DEGs in tight junction pathway, PI3K-Akt signaling pathway, actin cytoskeleton regulation, cGMP-PKG signaling pathway, Hippo signaling pathway, and TNF signaling pathway. Finally, we identified some hub genes (<i>GNAI3</i>, <i>GNAI1</i>, <i>JAK1</i>, <i>NDUFS8</i>, <i>CYC1</i>) related to oxidative phosphorylation. In summary, our results demonstrate that SIRT7 is pivotal for GPS-induced inflammatory responses, which represents a promising target resistant to GPS infection.</p>","PeriodicalId":12458,"journal":{"name":"Frontiers in Cellular and Infection Microbiology","volume":"15 ","pages":"1589199"},"PeriodicalIF":4.6,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12238765/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144599883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Host cellular transcriptional response to respiratory syncytial virus infection in HEp-2 cells: insights from cDNA microarray and quantitative PCR analyses.","authors":"Manoj K Pastey, Christopher Lupfer","doi":"10.3389/fcimb.2025.1613386","DOIUrl":"10.3389/fcimb.2025.1613386","url":null,"abstract":"<p><p>Respiratory syncytial virus (RSV) is a leading cause of lower respiratory tract infections in young children and elderly, worldwide and poses significant risks to immunocompromised individuals. To elucidate host-virus interactions at the transcriptional level, we analyzed differential gene expression in HEp-2 cells infected with RSV using cDNA microarray analysis complemented by quantitative PCR (qPCR). HEp-2 cells were infected with RSV at a multiplicity of infection of 1, and total RNA was isolated 24 hours post-infection for gene expression profiling. Radiolabeled cDNA probes from RSV-infected and mock-infected cells were hybridized to Atlas^® Human Cancer cDNA arrays, and differential gene expression was quantified by densitometry. We identified 12 host genes that were significantly upregulated in RSV-infected cells from the cDNA microarray (≥2-fold increase, <i>P</i><0.01), confirmed by qPCR, encompassing functional categories including cell cycle regulation, cytoskeletal organization, apoptosis modulation, immune evasion, and inflammation. Notably, the cyclin-dependent kinase inhibitor CDKN1A was induced ~14-fold, suggesting RSV triggers a host cell cycle arrest. The intermediate filament protein, vimentin was up ~6-fold, consistent with cytoskeletal rearrangements observed during viral syncytium formation. Anti-apoptotic MCL1 increased ~11-fold, while pro-apoptotic caspase-4 showed a more modest 1.6-fold rise, indicating a complex regulation of cell death pathways. We also observed marked upregulation of a fibronectin receptor subunit (~24-fold) and complement regulatory protein CD59 (~2-fold), highlighting potential mechanisms of enhanced cell-cell fusion and viral immune evasion. The proinflammatory cytokine interleukin-6 was elevated ~7-fold, underscoring the inflammatory response to RSV. These findings provide a global snapshot of the host transcriptomic response to RSV infection and yield insights into how RSV modulates host cellular machinery to favor viral replication and spread. Understanding these host-virus interactions may unveil novel targets for antiviral therapy and inform strategies to mitigate RSV disease pathogenesis.</p>","PeriodicalId":12458,"journal":{"name":"Frontiers in Cellular and Infection Microbiology","volume":"15 ","pages":"1613386"},"PeriodicalIF":4.6,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12234574/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144590834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}