Frontiers in Cellular and Infection Microbiology最新文献

{"title":"Changes in antioxidant capacity and gut microbiota in mice after intake of camel milk.","authors":"Jianwen Wang, Wanlu Ren, Shibo Liu, Zexu Li, Yaqi Zeng, Jun Meng, Xinkui Yao","doi":"10.3389/fcimb.2025.1621031","DOIUrl":"10.3389/fcimb.2025.1621031","url":null,"abstract":"<p><p>Fermented camel milk offers significant nutritional benefits, enriched with probiotics that generate bioactive compounds advantageous to human health. In order to investigate the effects of camel milk with different treatments on Antioxidant Capacity and Gut Microbiota in mice, 32 ICR mice were selected and randomly divided into 4 groups, including gavage with 10 mL/kg body weight of distilled water (DW Group), camel milk (CM Group), fermented camel milk (FCM Group), and pasteurized fermented camel milk (PFCM Group) every morning, respectively. After 28 days, liver and colon samples were collected to assess liver antioxidant capacity, and metagenomic analysis was performed on alterations in microbial community structures. Results demonstrated that all camel milk treatments elevated liver total protein levels while reducing MDA and SOD activity. In addition, the PFCM group had the highest total antioxidant capacity and the lowest SOD content. In addition, the intestinal microorganisms of mice changed at the phylum, genus and species levels after being gavaged with camel milk of different treatments. A total of 4732 microorganisms were identified, of which 259, 222, 116 and 164 were unique to the DW, CM, FCM and PFCM groups, respectively. The relative abundances of Adlercreutzia caecimuris, Adlercreutzia mucosicola and Enterorhabdus sp. P55 were significantly higher in the CM, FCM and PFCM groups than in the DW group, and the relative abundances of Parvibacter caecicola, Adlercreutzia muris and Roseburia sp. 1XD42-69 were significantly higher in the CM and PFCM groups than in the DW group. In addition, the relative abundances of Faecalibaculum rodentium, Alistipes muris and Limosilactobacillus reuteri were different between the CM and FCM groups. The results of the correlation analysis between the relative abundance of microbial species and antioxidant indices showed that Adlercreutzia mucosicola, Adlercreutzia muris, Lactobacillus acidophilus, and Enterorhabdus sp. P55 were significantly correlated with the antioxidant indices of mice. Further functional annotations indicated that these microorganisms might modulate antioxidant activity via metabolic and organismal systems. In summary, camel milk and fermented camel milk can play a positive role in regulating the intestinal flora of mice, thereby regulating the antioxidant capacity of mice and alleviating the effects of oxidative stress on the body. This study provides a scientific foundation for the further exploration and utilization of camel milk.</p>","PeriodicalId":12458,"journal":{"name":"Frontiers in Cellular and Infection Microbiology","volume":"15 ","pages":"1621031"},"PeriodicalIF":4.8,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12440968/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145085642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A one health perspective on multidrug-resistant bacterial infections: integrated approaches for surveillance, policy and innovation.","authors":"Chinenyenwa M D Ohia, Olutayo Israel Falodun, Lucky Icomiare Adebudo, Adeleye Solomon Bakarey","doi":"10.3389/fcimb.2025.1614232","DOIUrl":"10.3389/fcimb.2025.1614232","url":null,"abstract":"<p><p>Multidrug-resistant (MDR) bacterial infections represent a growing global health emergency, driven by interconnected human, animal, and environmental factors. This review adopts a One Health perspective to explore the transmission dynamics, operational integration, and innovative responses to antimicrobial resistance (AMR). Case studies from China, India, Nigeria, Thailand, and Brazil underscore the effectiveness of regulatory reforms, surveillance networks, and public engagement campaigns. Notably, Artificial Intelligence (AI) is transforming MDR management through real-time diagnostics and resistance prediction, though ethical concerns and infrastructure deficits in Low- and Middle-Income Countries (LMICs) remain barriers. Community-led initiatives, gender-sensitive education, and policy reforms are vital to curbing misuse and closing equity gaps. Despite successes, challenges such as fragmented governance, underfunded labs, and limited longitudinal research persist. A proactive, integrated One Health approach-linking clinical, environmental, and policy actions-is essential for reducing MDR burden. Investment in intersectoral surveillance, equitable AI deployment, and community empowerment is imperative for safeguarding antibiotics and ensuring global health resilience.</p>","PeriodicalId":12458,"journal":{"name":"Frontiers in Cellular and Infection Microbiology","volume":"15 ","pages":"1614232"},"PeriodicalIF":4.8,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12440884/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145085629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy of thymosin α1 for sepsis: a systematic review and meta-analysis of randomized controlled trials.","authors":"Bin Gu, Yu Zhou, Yao Nie, Luhao Wang, Liqun Liang, Zihuai Liao, Jingyi Wen, Xiangdong Guan, Minying Chen, Jianfeng Wu, Fei Pei","doi":"10.3389/fcimb.2025.1673959","DOIUrl":"10.3389/fcimb.2025.1673959","url":null,"abstract":"<p><strong>Background: </strong>Despite advances in understanding sepsis pathophysiology and extensive research, few treatments effectively target its underlying immune dysfunction. Thymosin α1 (Tα1) shows promise as an immunomodulator, but its impact on sepsis remains unclear.</p><p><strong>Methods: </strong>A search strategy was designed to include any prospective clinical studies using Tα1 for assessing 28-day mortality in patients with sepsis, excluding combination therapy studies. We conducted trial sequential analysis (TSA) to assess the robustness of meta-analyses findings. Heterogeneity of treatment effects (HTE) was conducted based on individual data from two multicenter randomized clinical trials (RCTs), with result credibility assessed through the instrument to assess the credibility of effect modification analyses (ICEMAN).</p><p><strong>Results: </strong>Out of 3003 identified studies, 11 RCTs met the inclusion criteria (967 patients in Tα1 group and 960 patients in control group). The comprehensive meta-analysis demonstrated a significant reduction in 28-day mortality associated with Tα1 administration (OR 0.73, 95%CI: 0.59-0.90, <i>P</i> = 0.003). Nonetheless, analyses of high-quality (OR 0.82, 95%CI: 0.65-1.03, <i>P</i> = 0.09) and multi-center (OR 0.86, 95%CI: 0.68-1.08, <i>P</i> = 0.20) subgroups did not reveal a mortality benefit. The HTE analysis of multiple subgroups in two large RCTs (representing 75% of the total patients) showed heterogeneity. Potential benefits were noted in subgroups of cancer (moderate credibility), diabetes (low credibility), and coronary heart disease (low credibility). Furthermore, the trial sequential analysis (TSA) suggests that the current sample size is inadequate.</p><p><strong>Conclusion: </strong>Tα1 has the potential to decrease 28-day mortality rates in patients with sepsis; however, it is crucial to recognize that its efficacy differs among various subgroups. These observations underscore the significance of personalized immunotherapy strategies in forthcoming clinical trials.</p><p><strong>Systematic review registration: </strong>https://www.crd.york.ac.uk/prospero/, identifier CRD42024628937.</p>","PeriodicalId":12458,"journal":{"name":"Frontiers in Cellular and Infection Microbiology","volume":"15 ","pages":"1673959"},"PeriodicalIF":4.8,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12440967/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145085647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"3D human epithelial models: a promising platform for studying <i>Candida</i> infections and novel antifungal therapeutic options.","authors":"Cristina Schöpf, Florentine Marx","doi":"10.3389/fcimb.2025.1680261","DOIUrl":"10.3389/fcimb.2025.1680261","url":null,"abstract":"","PeriodicalId":12458,"journal":{"name":"Frontiers in Cellular and Infection Microbiology","volume":"15 ","pages":"1680261"},"PeriodicalIF":4.8,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12440887/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145085672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Single-nucleus RNA sequencing reveals HBV-driven metabolic reprogramming and TIMP1-mediated fibrosis in human-liver-chimeric mice.","authors":"Xiaonan Ren, Cong Wang, Boyin Qin, Hua Yang, Min Wu, Zhanqing Zhang, Wei Lu, Chao Wang, Yabin Liu, Xiaonan Zhang, Xiaohui Zhou","doi":"10.3389/fcimb.2025.1654903","DOIUrl":"10.3389/fcimb.2025.1654903","url":null,"abstract":"<p><strong>Introduction: </strong>Hepatitis B virus (HBV) infection remains a leading cause of chronic liver disease, cirrhosis, and hepatocellular carcinoma worldwide. Despite advances in antiviral therapies, the mechanisms underlying HBV-induced metabolic reprogramming and liver fibrosis remain poorly understood.</p><p><strong>Methods: </strong>We employed single-nucleus RNA sequencing (snRNA-seq) which is particularly suitable for hepatocytic sequencing to dissect the transcriptional landscape of HBV-infected and uninfected hepatocytes in humanized URG mice (Hu-URG).</p><p><strong>Results and discussion: </strong>Chronic HBV infection was successfully established in Hu-URG mice, with progressive increases in serum HBV DNA, HBsAg, and HBeAg levels. snRNA-seq revealed distinct human hepatocyte clusters (clusters 9, 16, 23) characterizing elevated expression of metabolic genes (<i>ALB, UGT2B17, CYP2A6</i>) in HBV-infected cells, while HBV-uninfected cells exhibited upregulation of TIMP1 and pro-fibrotic pathways. Immunofluorescence and histological analyses confirmed that HBV-uninfected hepatocytes (HBsAg^-) displayed higher TIMP1 expression and reduced albumin (hALB) levels, correlating with increased collagen deposition in HBV-hu-URG mice. Notably, this TIMP1⁺HBsAg^-hALB^low phenotype was also observed in liver biopsies from chronic HBV patients, underscoring its clinical relevance. Our findings highlight HBV-driven metabolic adaptation and identify TIMP1 as a potential mediator of fibrosis in uninfected hepatocytes, offering novel insights into HBV pathogenesis and therapeutic targeting.</p>","PeriodicalId":12458,"journal":{"name":"Frontiers in Cellular and Infection Microbiology","volume":"15 ","pages":"1654903"},"PeriodicalIF":4.8,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12436295/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145080146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Bacillus velezensis</i> NC-B4 as a promising antifungal agent for biocontrol of <i>Candida auris</i>.","authors":"Chunxi Yang, Chaoyu Cui, Yanru Chen, Zimei Peng","doi":"10.3389/fcimb.2025.1515537","DOIUrl":"10.3389/fcimb.2025.1515537","url":null,"abstract":"<p><strong>Introduction: </strong><i>Candida auris</i>, known as the \"super fungus\", is commonly existed in hospital. The treatment of <i>C. auris</i> infection is difficult for its multi-drug resistance and difficult to accurately detect. The use of synthetic antibacterial agents has caused major problems such as drug-resistance and environment pollution and negatively affects non-target species. Microbial biocontrol agents (probiotics) are needed for fungal infection. <i>Bacillus</i> and related genera produce a variety of bioactive substances. As probiotics, it has been widely studied in the field of medicine and is a novel microbial factor for biological control.</p><p><strong>Methods: </strong><i>B. velezensis</i> NC-B4 was isolated using gradient dilution method. Then it was identified by phylogenetic analysis and physiological and biochemical characteristics. The antibacterial mechanism of NC-B4 was explored by detecting cellulase, protease and genomic analysis. Then antimicrobial effects were analyzed by detecting the growth and biofilm of <i>C. auris</i> BJCA001. Finally, the cytotoxicity and the protective effect on mice were analyzed by cell line and mouse systemic infection models.</p><p><strong>Results: </strong>We isolated <i>B. velezensis</i> NC-B4, which showed cellulase, protease enzyme activity and antimicrobial effects against human pathogenic fungi by inhibiting the growth of Candida auris, <i>Cryptococcus neoformans, Candida albicans</i> and mycelial fungus. <i>B. velezensis</i> NC-B4 inhibited the biofilm formation and growth of C. auris. <i>B. velezensis</i> NC-B4 has a protective effect against the toxicity of Candida auris in A549 cell line and mouse systemic infection models. The complete genome of <i>B. velezensis</i> NC-B4 was 3.93 Mb with a 46.5% G+C content and possessed the macrolactin H, bacillaene, fengycin, difficidin, bacillibactin and bacilysin biosynthesis cluster, which known as key factors in biological control.</p><p><strong>Discussion: </strong>The results of the present study indicated that <i>B. velezensis</i> NC-B4 has antimicrobial properties for its cellulase, protease and antibacterial secondary metabolites, thereby inhibiting the growth of pathogenic bacteria and the formation of biofilms. <i>B. velezensis</i> NC-B4 is expected to be developed as a source for probiotics or new antibiotics.</p>","PeriodicalId":12458,"journal":{"name":"Frontiers in Cellular and Infection Microbiology","volume":"15 ","pages":"1515537"},"PeriodicalIF":4.8,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12436363/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145080148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Global distribution and temporal analysis of R70Q/H oncogenic mutations in hepatitis C virus subtype 1b core protein.","authors":"Gabriela T M Nunes, Natalia M Araujo","doi":"10.3389/fcimb.2025.1619865","DOIUrl":"10.3389/fcimb.2025.1619865","url":null,"abstract":"<p><p>The hepatitis C virus (HCV) core protein is crucial in viral pathogenesis and hepatocarcinogenesis. Amino acid substitutions at position 70, particularly R70Q and R70H, are associated with an increased risk of hepatocellular carcinoma (HCC) and partial resistance to interferon-based therapy in genotype 1b infections. However, the global and temporal dynamics of these oncogenic mutations remain poorly understood. In this study, we analyzed 3,218 publicly available HCV subtype 1b core sequences to investigate the global distribution of R70Q/H mutations and their evolution across therapeutic eras. Our findings reveal notable regional disparities, with R70Q prevalence highest in Western Europe (77.4%) and Northern America (70.4%), while R70H was most frequent in Central America (45%). Temporal analysis of 1,351 dated sequences showed a significant decline in R70Q/H frequency during the pegylated interferon plus ribavirin era (2001-2010: 24%) compared to the conventional interferon period (1989-2000: 39%; <i>p</i> = 0.0081), followed by a resurgence in the direct-acting antivirals (DAAs) era (2014-present: 43%; <i>p</i> = 0.0183). These temporal shifts, including both the decline and resurgence, suggest a complex interplay between treatment-related selective forces, viral diversity, host factors, and possibly sampling bias. Our results underscore the need for regional molecular surveillance to guide HCC monitoring in HCV subtype 1b patients with R70Q/H mutations, even after viral clearance, and to inform targeted prevention strategies in high-prevalence areas.</p>","PeriodicalId":12458,"journal":{"name":"Frontiers in Cellular and Infection Microbiology","volume":"15 ","pages":"1619865"},"PeriodicalIF":4.8,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12436453/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145080084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbiota composition and intestinal barrier function modulated by tamsulosin and <i>Lactococcus lactis</i> in a cirrhosis rat model.","authors":"Silvia Valeria Padilla-García, Abraham Loera-Muro, Martín Humberto Muñoz-Ortega, David Alejandro Hernández-Marín, Javier Ventura-Juárez, Sandra Luz Martínez-Hernández","doi":"10.3389/fcimb.2025.1624065","DOIUrl":"10.3389/fcimb.2025.1624065","url":null,"abstract":"<p><strong>Introduction: </strong>The pathological progression of cirrhosis disrupts the gut-liver axis. <i>Lactococcus lactis</i> (<i>L. lactis</i>) exhibits immunomodulatory properties and an ability to enhance intestinal barrier function. It has been demonstrated that tamsulosin has antifibrotic and anti-inflammatory effects in hepatic injury models. This study evaluated the effect of a tamsulosin and <i>L. lactis</i> co-treatment on the recovery of microbiota and intestinal barrier integrity in a Wistar rat liver cirrhosis model.</p><p><strong>Material and methods: </strong>Male Wistar rats were administered CCl₄ intraperitoneally for 4 weeks. Subsequently, rats received tamsulosin, <i>L. lactis</i>, or both, orally for 2 weeks. The intestinal microbiota was assessed using 16S rRNA gene sequencing. Intestinal barrier integrity was evaluated using qPCR and Western blot for proteins ZO-1, occludin, and claudin-2. Bacterial translocation was evaluated by endotoxin concentration, bacterial DNA, and microbial culture of extraintestinal tissues. Finally, hepatic, intestinal histology, and liver function markers were analyzed.</p><p><strong>Results: </strong><i>L. lactis</i> and its combination with tamsulosin (T/<i>L. lactis</i>) increased microbial diversity and promoted a balanced gut microbiota characterized by a <i>Firmicutes</i> predominance followed by <i>Proteobacteria</i> and reduced <i>Clostridia</i> and <i>Gammaproteobacteria</i> levels. <i>L. lactis</i> group upregulated ZO-1 and occludin expression, while no significant changes were observed with tamsulosin or T/<i>L. actis</i> groups, nonetheless, intestinal morphology resembled that of healthy controls. Bacterial translocation analysis revealed no endotoxins, bacterial DNA, or bacteria in extraintestinal tissues. Both treatments also improved hepatic and intestinal histology, with partial liver function recovery.</p><p><strong>Conclusión: </strong>Findings such as reduced bacterial translocation, lower systemic endotoxin levels, improved intestinal morphology, and modulation of gut microbiota composition suggest that both agents (<i>L. lactis</i> and tamsulosin), particularly in combination, exert positive effects on the intestinal barrier in cirrhosis.</p>","PeriodicalId":12458,"journal":{"name":"Frontiers in Cellular and Infection Microbiology","volume":"15 ","pages":"1624065"},"PeriodicalIF":4.8,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12436309/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145080124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phylogenetic evidence for nationwide expansion <i>Brucella melitensis</i> lineages drives the re-emerging and epidemic of human brucellosis in Jiangsu, China.","authors":"Weixiang Wang, Zhou Lu, Ge Teng, Zikang Yan, Lan Huang, Zhongming Tan, Zhiguo Liu, Songning Ding, Zhenjun Li","doi":"10.3389/fcimb.2025.1603234","DOIUrl":"10.3389/fcimb.2025.1603234","url":null,"abstract":"<p><strong>Objective: </strong>Human brucellosis has re-emerged as a major public health threat in Jiangsu Province, but the sources and transmission dynamics of circulating strains remain poorly understood.</p><p><strong>Methods: </strong>In this study, we integrated conventional biotyping, whole-genome sequencing single-nucleotide polymorphism (WGS-SNP) analysis, and core genome multilocus sequence typing (cgMLST) to investigate the phylogenetic relationships of <i>Brucella melitensis</i> in the region.</p><p><strong>Results: </strong>Among 89 isolates analyzed, all were confirmed as <i>B. melitensis</i> (16 as biovar 1 and 73 as biovar 3), with a widespread geographic distribution across 15 cities in Jiangsu and adjacent areas, indicating extensive regional dissemination. All strains belonged to sequence type 8 (ST8) and genotype group II, clustering within the East Mediterranean lineage. Genomic resolution classified these strains into five SNP clades (C-I to C-V) and 17 SNP-based genotypes (STs), revealing a ladder-like phylogenetic structure. The lack of distinct geographic clustering suggests frequent cross-regional transmission, likely facilitated by the movement of infected sheep and goats. Phylogenomic analysis through cgMLST revealed distinct clustering of the 17 STs into two major groups (G-I and G-II), with 15 STs (88.2%) showing high genetic concordance between Jiangsu isolates and strains from China's northeastern and northwestern. This compelling genomic evidence establishes that the current human brucellosis epidemic in Jiangsu is being driven by the nationwide expansion of dominant <i>B. melitensis</i> lineages.</p><p><strong>Conclusion: </strong>The findings provide crucial insights into the infection sources and interregional transmission dynamics of brucellosis in southern China, highlighting the significant role of domestic animal movement in pathogen dissemination, demanding coordinated cross-regional interventions including strict implementing intervention strategies and enhance disease surveillance.</p>","PeriodicalId":12458,"journal":{"name":"Frontiers in Cellular and Infection Microbiology","volume":"15 ","pages":"1603234"},"PeriodicalIF":4.8,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12436464/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145080107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Portable, precise, and RNA extraction-free: RT-RAA technology for rapid early RSV identification and prevention.","authors":"Zhenfei Wang, Jing Zhang, Xiao He, Chunming Wang, Zhenyue Li, Zitong Yang, Cheng Zhang, Tingli Fan, Kai Su","doi":"10.3389/fcimb.2025.1640503","DOIUrl":"10.3389/fcimb.2025.1640503","url":null,"abstract":"<p><p>The Respiratory Syncytial Virus (RSV) is a significant agent linked to respiratory infections, representing a considerable health risk for vulnerable populations, including infants, older adults, and those with weakened immune systems. This research successfully introduces an RNA extraction-free rapid detection technique for RSV utilizing real-time reverse transcription recombinase-aided amplification (RT-RAA) technology. Through the crafting of specific primers and probes, this approach enables precise identification of RSV without any interference from other prevalent respiratory viruses. Tests for sensitivity indicated that the detection threshold at a 95% confidence interval was 159 copies per reaction, while the visual detection limit was found to be 1,177 copies per reaction. Testing on clinical samples demonstrated a high degree of consistency with reverse transcription quantitative real-time PCR (RT-qPCR), achieving a Kappa value of 1, which signifies excellent correlation. Furthermore, the amplified products from RT-RAA can be seen with the aid of a portable blue light device, rendering this method appropriate for rapid detection in settings where resources are limited. A total of 265 clinical samples were tested, and the results showed 100% concordance with RT-qPCR. Compared with rapid antigen detection tests (RADTs), RT-RAA exhibited significantly higher sensitivity (100% <i>vs</i>. 93.8%). The rapid detection method for RSV using RT-RAA offers solid technical assistance for the early identification and prevention of RSV.</p>","PeriodicalId":12458,"journal":{"name":"Frontiers in Cellular and Infection Microbiology","volume":"15 ","pages":"1640503"},"PeriodicalIF":4.8,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12436364/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145080103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}