Frontiers in Cellular and Infection Microbiology最新文献

{"title":"Clinicopathological characteristics and diagnostic performance of metagenomic pathogen detection technology in mycobacterial infections among HIV patients.","authors":"Man Li, Jiamin Chen, Liang Zhang, Xiangmei Chen, Jianfeng Zhou, Feifei Liu, Xingang Zhou, Jiang Xiao, Kun Yang, Liming Qi, Xiaoyi Han, Ting Liu, Hongxin Zhao, Zhen Zhou, Xiaoyou Chen, Lei Sun","doi":"10.3389/fcimb.2025.1584189","DOIUrl":"10.3389/fcimb.2025.1584189","url":null,"abstract":"<p><strong>Background: </strong>Mycobacterial infections represent a major cause of morbidity and mortality in HIV-infected individuals. This study evaluated diagnostic techniques for mycobacterial identification and compared clinicopathological features between HIV-positive and HIV-negative patients.</p><p><strong>Methods: </strong>We analyzed 88 tissue samples (with 41 matched blood and 28 sputum samples) using histopathology (HE and acid-fast staining), bacterial culture, MTB-PCR (sputum/biopsy), PCR-reverse dot blot hybridization (RDBH), and metagenomic pathogen detection technology (MetaPath™). Logistic regression analyses were performed to identify factors affecting detection rates.</p><p><strong>Results: </strong>Mycobacterial infection was detected in 95.5% (84/88) of patients. Among HIV-positive patients (n=63), 46% (29/63) had <i>Mycobacterium tuberculosis</i> (MTB) infections, and 44% (28/63) had non-tuberculous mycobacteria (NTM) infections, significantly higher than the 20% (5/25) NTM rate in HIV-negative patients. Univariate analysis identified HIV-positive status (<i>p</i>=0.009), lymph node involvement (<i>p</i>=0.020), and positive MetaPath™ results (<i>p</i>=0.002) as significant predictors of detection, while multivariate analysis confirmed these as independent factors (<i>p</i>=0.036; <i>p</i>=0.042; <i>p</i>=0.006). Lymph nodes were the most common infection site in HIV-positive patients (42.9%, 27/63), while lung tissue predominated in HIV-negative patients (48%, 12/25). MetaPath™ demonstrated superior sensitivity and specificity for detecting both MTB and NTM. Biopsy samples provided higher diagnostic accuracy than sputum or blood for lung and lymph node infections, but not for brain. In HIV-positive patients, NTM infections showed significantly more granuloma formation (<i>p</i>=0.032) and foam cells (<i>p</i>=0.005), but less necrosis (<i>p</i>=0.0005) compared to MTB infections. No significant differences were observed in HIV-negative patients.</p><p><strong>Conclusions: </strong>MetaPath™ is a highly effective diagnostic tool for mycobacterial infections, particularly in tissue biopsies. HIV-positive status, lymph node involvement, and MetaPath™ positivity independently predict mycobacterial detection. HIV-positive patients exhibit distinct clinicopathological features, emphasizing the need for tailored diagnostic and therapeutic approaches based on immune status.</p>","PeriodicalId":12458,"journal":{"name":"Frontiers in Cellular and Infection Microbiology","volume":"15 ","pages":"1584189"},"PeriodicalIF":4.6,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12069358/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143989328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Editorial: Interaction of microbiota and metabolomic disorders.","authors":"Jorge A M Pereira","doi":"10.3389/fcimb.2025.1597214","DOIUrl":"https://doi.org/10.3389/fcimb.2025.1597214","url":null,"abstract":"","PeriodicalId":12458,"journal":{"name":"Frontiers in Cellular and Infection Microbiology","volume":"15 ","pages":"1597214"},"PeriodicalIF":4.6,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12069356/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143972322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Egg-driven immunosuppression and granuloma zonation in Peyer's patches of mice with <i>Schistosoma japonicum</i> infection.","authors":"Linzhu Li, Jing Wu, Guangxu Cao, Jiakai Yao, Yanping Miao, Yanglin Zhuang, Yushen Xiang, Xiaolin Zhong, Yicong Liu, Fubo Chen, Yalei Dai, Yang Dai, Xindong Xu, Qingfeng Zhang","doi":"10.3389/fcimb.2025.1587166","DOIUrl":"10.3389/fcimb.2025.1587166","url":null,"abstract":"<p><p>Egg granulomas caused by <i>Schistosoma japonicum</i> (<i>S. japonicum</i>) are important causes of morbidity and mortality in schistosomiasis. The intestine plays a crucial role in the complete life cycle of <i>S. japonicum</i>; eggs are transported through the intestine and excreted with feces. During this process, the interaction between the eggs and the intestine can trigger a strong intestinal immune system response and cause inflammation. Eggs in the intestine preferentially accumulate in Peyer's patches (PPs). However, the cellular composition of intestinal granulomas and the impacts of egg deposition on the immune function of PPs remain poorly understood. Using a mouse model of <i>S. japonicum</i> infection, we revealed that the deposition of eggs disrupted the structure of PPs, resulting in immunosuppression. We further characterized the cellular composition of intestinal granulomas, revealing a layered distribution of neutrophils, macrophages, T cells, and B cells, with marked neutrophil accumulation. Single-cell RNA sequencing revealed that egg deposition drives B-cell apoptosis, T-cell exhaustion, and activation of fibrotic pathways in myeloid cells, collectively impairing PP function. In conclusion, the layered cellular architecture of intestinal granulomas in PPs suggests a unique immune microenvironment of egg-driven immunosuppression and fibrotic remodeling, and the identification of fibrotic pathways in myeloid cells provides a potential therapeutic target to alleviate fibrosis in patients with <i>S. japonicum</i> infection.</p>","PeriodicalId":12458,"journal":{"name":"Frontiers in Cellular and Infection Microbiology","volume":"15 ","pages":"1587166"},"PeriodicalIF":4.6,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12070193/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143973127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Plasma thrombomodulin is a valuable biomarker to predict the severity of hemorrhagic fever with renal syndrome caused by the Hantaan virus.","authors":"Han-Dong Zhao, Yan Zhang, Xiao-Hong Wang, Hong-Bo Qian, Tong-Bo Yu, Peng Li, Kang-Xiao Ma, Hong-Li Liu","doi":"10.3389/fcimb.2025.1563621","DOIUrl":"https://doi.org/10.3389/fcimb.2025.1563621","url":null,"abstract":"<p><strong>Objectives: </strong>This study aimed to investigate the Thrombomodulin (TM) levels in patients who suffered hemorrhagic fever with renal syndrome (HFRS) of varying severities, and to evaluate the predictive properties of TM for the seriousness of HFRS, thereby providing a clue for the monitoring and management of this patients in the future.</p><p><strong>Methods: </strong>Chemiluminescence was used to determine the concentrations of TM in 196 patients with HFRS and 49 healthy controls. Conventional testing techniques were used to test the basic clinical reference values for leukocytes, platelets (PLT), C-reactive protein (CRP), creatine (Cr), uric acid (UA), and urea, and the values for activated partial thromboplastin time, prothrombin time, and fibrinogen. The colloidal gold method was used to measure HFRS antibody levels in the patients. The correlation of TM with conventional parameters was assessed using Spearman correlation analysis, and ordinal logistic regression analysis was used to analyze the severity risk factors. The predictive potency of TM for HFRS patients' severity was evaluated by receiver operating characteristics (ROC) curve analysis.</p><p><strong>Results: </strong>The concentrations of TM increased with disease severity and peaked in the critical type patients. In addition, plasma levels of TM were proportionally correlated with the levels of leukocytes (<i>r</i>= 0.4218; <i>p</i><0.01), creatine (<i>r</i>= 0.3797; <i>p</i><0.01), urea (<i>r</i>= 0.3763; <i>p</i><0.01), uric acid (<i>r</i>= 0.3624; <i>p</i><0.01), and C-reactive protein (<i>r</i>= 0.2767; <i>p</i><0.01). Conversely, there was an inverse correlation between TM, platelet counts (<i>r</i>= -0.4509; <i>p</i><0.01), and fibrinogen levels (<i>r</i>= -0.2431; <i>p</i><0.01). Furthermore, TM demonstrated significant predictive value for the severity of HFRS with an area under the ROC curve (AUC) of 0.872(95% <i>CI</i>: 0.822-0.921, <i>P</i><0.001).</p><p><strong>Conclusions: </strong>TM levels are associated with HFRS severity, suggesting that TM detection might be beneficial for monitoring the status and effective management of HFRS patients.</p>","PeriodicalId":12458,"journal":{"name":"Frontiers in Cellular and Infection Microbiology","volume":"15 ","pages":"1563621"},"PeriodicalIF":4.6,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12066576/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143983814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic analysis of prophages in 44 clinical strains of <i>Pseudomonas aeruginosa</i> isolated in Saudi Arabia.","authors":"Ahlam Alsaadi, Mohammed Imam, Abdulrahman A Alghamdi, Safia S Aljedani, Amal Alsari, Haya Aljami, Mohammad Bosaeed","doi":"10.3389/fcimb.2025.1563781","DOIUrl":"https://doi.org/10.3389/fcimb.2025.1563781","url":null,"abstract":"<p><p>Prophages are bacteriophages that integrate their genomes into the bacterial chromosome. This research aimed to analyze and characterize prophages integrated into 44 <i>Pseudomonas aeruginosa</i> strains isolated from tertiary hospitals in Saudi Arabia. A total of 97 intact prophages were identified among clinical strains, with 16 prophages found present in more than one strain simultaneously. All prophages were found to have lengths ranging from 7.7 kb to 74.1 kb, and their GC content was found to be between 49.91% and 64.9%. Our findings show that prophages are present in the majority of the isolated <i>P. aeruginosa</i> strains (41 out of 44). Additionally, several proteins related to viral defense (toxin/antitoxin modules and proteins against restriction-modification enzymes) were identified, supporting the idea that prophages influence bacterial pathogenesis and anti-phage defenses.</p>","PeriodicalId":12458,"journal":{"name":"Frontiers in Cellular and Infection Microbiology","volume":"15 ","pages":"1563781"},"PeriodicalIF":4.6,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12066637/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143961196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling <i>Wolbachia</i> transcriptomic signature in the arboviral vector <i>Aedes aegypti</i>.","authors":"Sebastián Mejías, Natalia E Jiménez, Carlos Conca, J Cristian Salgado, Ziomara P Gerdtzen","doi":"10.3389/fcimb.2025.1538459","DOIUrl":"https://doi.org/10.3389/fcimb.2025.1538459","url":null,"abstract":"<p><strong>Introduction: </strong>The mosquito <i>Aedes aegypti</i> is the main vector of arboviral diseases such as dengue and imposes a global health burden. A promising control strategy is to infect <i>A. aegypti</i> populations with <i>Wolbachia</i>, a genus of intracellular bacteria capable of blocking arboviral infections. Enhancing and preserving the efficacy of this method will depend on a solid mechanistic knowledge of the <i>A. aegypti-Wolbachia</i> symbiosis. By identifying differences between <i>Wolbachia</i>-infected and uninfected <i>A. aegypti</i>, previous transcriptomic studies proposed a wide range of symbiotic interactions, but a systematic identification of consistent effects across datasets is still missing.</p><p><strong>Methods: </strong>To identify <i>A. aegypti</i> genes and functions consistently affected by <i>Wolbachia</i>, we performed differential expression and functional enrichment analysis on published transcriptomic datasets, followed by a meta-analysis of the obtained <i>p-values</i> using the maxP method. Six datasets were retrieved from Gene Expression Omnibus, Sequence Read Archive and ArrayExpress (last searched in July 2024, considering lack of replication as the exclusion criteria). After discarding one dataset from <i>w</i>AlbB-infected cell line due to poor mapping to the <i>A. aegypti</i> genome, the data comprised adult female <i>A. aegypti</i> heads, muscles, carcasses, midguts and bodies, and Wolbachia strains wMel and wMelPop.</p><p><strong>Results and discussion: </strong>Meta-analysis revealed 10 and 21 consistently down- and upregulated host genes, some of which have escaped the focus of previous research, including the consistently downregulated exonuclease <i>AAEL009650</i> which has a pro-dengue virus homolog in <i>Drosophila</i>. At the function level, we found consistent upregulation of electron transport chain (ETC), carbohydrate transport and serine-type peptidase activity and inhibition, and downregulation of DNA replication. ETC upregulation suggests an alternative mechanism for Wolbachia's induction of antiviral oxidative stress, previously attributed to dual- and NADPH-oxidases which here showed downregulation or no regulation. Through analysis of previously published datasets, this work identifies promising molecular and functional targets for future studies aimed at elucidating the most fundamental mechanisms of the <i>A. aegypti-Wolbachia</i> symbiosis.</p>","PeriodicalId":12458,"journal":{"name":"Frontiers in Cellular and Infection Microbiology","volume":"15 ","pages":"1538459"},"PeriodicalIF":4.6,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12066770/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143977548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence of <i>Chlamydia trachomatis</i>, <i>Neisseria gonorrhoeae</i>, and <i>Ureaplasma urealyticum</i> infections in males and females of childbearing age in Chengdu, China.","authors":"Yuwei Li, Zhiyong Liao, Qin Wang, Weijun He, Yao Deng, Chenggui Liu","doi":"10.3389/fcimb.2025.1566163","DOIUrl":"https://doi.org/10.3389/fcimb.2025.1566163","url":null,"abstract":"<p><strong>Background: </strong>Sexually transmitted infections (STIs) are a global public health issue, due to their high prevalence and potential impact on pregnancy outcome and fetal health. The objective of this study is to investigate the prevalence of STI-causative pathogens including <i>Chlamydia trachomatis</i> (CT), <i>Neisseria gonorrhoeae</i> (NG) and <i>Ureaplasma urealyticum</i> (UU) as well as the epidemiological characteristics of STIs among males and females of childbearing age in Chengdu.</p><p><strong>Methods: </strong>This retrospective cross-sectional study involved 15,055 participants of childbearing age including 7,235 males and 7,820 females. All specimens of participants were tested for CT, NG, and UU by nucleic acid amplification tests (NAATs) methods.</p><p><strong>Results: </strong>27.80% of the participants were infected with at least one of the three pathogens, with significantly higher overall prevalence in females (45.22%) than males (8.98%, <i>p</i><0.001). Multivariate logistic regression analysis demonstrated that gender was independently associated with both CT positivity (females vs males, OR 2.276, 95% CI 1.724-3.005, <i>p</i><0.001) and UU positivity (females vs males, OR 8.079, 95% CI 7.183-9.086, <i>p</i><0.001). Single infections predominated in both males and females (males: 98.15%; females: 93.16%), while mixed infections were more frequent in females (6.84%) than in males (1.85%). CT prevalence was highest in both males and females aged 18-24, followed by aged 25-30 and 31-35. Among aged 18-24, the prevalence of UU in males and NG and UU in females were also higher. Compared to other age groups, mixed infections (CT+NG, CT+UU, NG+UU, CT+NG+UU) were also highest in females aged 18-24. Compared to other clinical diagnostic groups, The prevalence of CT, NG and mixed infection of CT+NG was highest in both males and females in the urogenital inflammation group (<i>p</i><0.05).</p><p><strong>Conclusions: </strong>Among the population of childbearing age in Chengdu, China, the prevalence among females was significantly higher than that among males. Single infections predominated in both males and females, while mixed infections occurred more frequently in females. STIs were more prevalent in sexually active young people aged 18-35, especially in the 18-24 age group. CT and NG infections in both males and females may cause urogenital inflammation, and mixed infections of CT+NG further elevate the risk of inflammatory responses.</p>","PeriodicalId":12458,"journal":{"name":"Frontiers in Cellular and Infection Microbiology","volume":"15 ","pages":"1566163"},"PeriodicalIF":4.6,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12066496/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143986237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Progress on the mechanism of intestinal microbiota against colorectal cancer.","authors":"Guoqiang Xing, Yu Cui, Zhiyue Guo, Bing Han, Guogang Zhao","doi":"10.3389/fcimb.2025.1565103","DOIUrl":"https://doi.org/10.3389/fcimb.2025.1565103","url":null,"abstract":"<p><p>The intestinal microbiota plays a crucial role in the occurrence and development of colorectal cancer, and its anti - colorectal cancer mechanism has become a research hotspot. This article comprehensively expounds on the molecular mechanisms of the intestinal microbiota in anti - colorectal cancer, including aspects such as immune regulation, activation of carcinogenic signaling pathways (it should be noted that it is more reasonable to be \"inhibition of carcinogenic signaling pathways\"), metabolite - mediated effects, and maintenance of intestinal barrier function. At the same time, it explores the roles and potential mechanisms of intervention methods such as probiotic supplementation therapy, immunotherapy, and fecal microbiota transplantation. In addition, it analyzes the impact of the intestinal flora on the therapeutic efficacy of colorectal cancer. The existing research results are summarized, and the future research directions are prospected, with the aim of providing new theoretical bases and treatment ideas for the prevention and treatment of colorectal cancer.</p>","PeriodicalId":12458,"journal":{"name":"Frontiers in Cellular and Infection Microbiology","volume":"15 ","pages":"1565103"},"PeriodicalIF":4.6,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12066583/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143986238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical evaluation of personalized <i>Helicobacter pylori</i> treatment guided by PCR detection from fecal samples: a real-world study.","authors":"Ruolin Peng, Zhimei Zhang, Chuchu Yang, Zhengyuan Xu, Jiabin Wang, Lei Chen, Sujun Gao, Jian Tao, Meijuan Xi, Xiaofeng Ye, Lu Shen, Qiang Zhan, Lijia Din, Jun Wang, Rong Ou, Jianhua Cui, Lin Chen, Xiaodan Xu, Gongyu Zhang, Chunyan Xu, Jing Du, Guangxia Chen, Xinxin Zhao, Lamei Xu, Min Xu, Zhenyu Zhang","doi":"10.3389/fcimb.2025.1519804","DOIUrl":"https://doi.org/10.3389/fcimb.2025.1519804","url":null,"abstract":"<p><strong>Background: </strong>Growing antibiotic resistance in <i>Helicobacter pylori</i> (<i>H. pylori</i>) diminishes eradication therapy effectiveness, emphasizing the need for accurate, non-invasive diagnostic techniques. This study aims to assess the clinical utility of PCR analysis of fecal samples for detecting antibiotic resistance in guiding personalized treatment for <i>H. pylori</i> infection.</p><p><strong>Methods: </strong>A retrospective, observational study was conducted across 13 hospitals within Jiangsu Province. Fecal samples were analyzed using fluorescence PCR for the <i>23S rRNA</i> and <i>gyrA</i> genes, indicating clarithromycin and levofloxacin resistance. Then, individualized eradication recommendations were proposed for the <i>H. pylori</i>-positive patients. A follow-up was performed one year later to evaluate the eradication outcomes in a routine medical environment, with participants having provided informed consent.</p><p><strong>Results: </strong>A total of 387 participants completed the eradication treatment, with an overall success rate of 91.0% (352/387, 95% CI: 87.6%-93.6%). Among them, 310 individuals received a 14-day course of bismuth quadruple therapy (BQT), achieving an eradication rate of 90.0% (279/310, 95% CI: 86.1%-93.1%). 77 participants were treated with high-dose dual therapy (HDDT) for the same duration, resulting in a slightly higher eradication rate of 94.8% (73/77, 95% CI: 87.2%-98.6%), although this difference was not statistically significant (<i>P</i>=0.188). No significant differences in eradication rates were observed among various BQT antibiotic combinations (<i>P</i>=0.208). The eradication rates for HDDT, based on either vonoprazan or esomeprazole, were 96.8% (61/63, 95% CI: 89.0%-99.6%) and 85.7% (12/14, 95% CI: 57.2%-98.2%), respectively, without a significant difference (<i>P</i>=0.304).</p><p><strong>Conclusion: </strong>PCR detection from fecal samples targeting the resistance genes of <i>H. pylori</i> is effective in guiding personalized treatments, highlighting its clinical utility and potential for broader application.</p>","PeriodicalId":12458,"journal":{"name":"Frontiers in Cellular and Infection Microbiology","volume":"15 ","pages":"1519804"},"PeriodicalIF":4.6,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12066622/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143984252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances in whole genome sequencing for foodborne pathogens: implications for clinical infectious disease surveillance and public health.","authors":"Emílio Gomes, Daniela Araújo, Teresa Nogueira, Ricardo Oliveira, Sónia Silva, Lorena V N Oliveira, Nuno F Azevedo, Carina Almeida, Joana Castro","doi":"10.3389/fcimb.2025.1593219","DOIUrl":"https://doi.org/10.3389/fcimb.2025.1593219","url":null,"abstract":"<p><p>Foodborne outbreaks affecting millions of people worldwide are a significant and growing global health threat, exacerbated by the emergence of new and increasingly virulent foodborne pathogens. Traditional methods of detecting these outbreaks, including culture-based techniques, serotyping and molecular methods such as real-time PCR, are still widely used. However, these approaches often lack the precision and resolution required to definitively trace the source of an outbreak and distinguish between closely related strains of pathogens. Whole genome sequencing (WGS) has emerged as a revolutionary tool in outbreak investigations, providing high-resolution, comprehensive genetic data that allows accurate species identification and strain differentiation. WGS also facilitates the detection of virulence and antimicrobial resistance (AMR) genes, providing critical insight into the potential pathogenicity, treatment/control options and risks of spreading foodborne pathogens. This capability enhances outbreak surveillance, source tracing and risk assessment, making WGS an increasingly integrated component of public health surveillance systems. Despite its advantages, the widespread implementation of WGS faces several pressing challenges, including high sequencing costs, the need for specialized bioinformatics expertise, limited computational infrastructure in resource-constrained settings, and the standardization of data-sharing frameworks across regulatory and public health agencies. Addressing these barriers is crucial to maximizing the impact of WGS on foodborne disease surveillance. Even so, WGS is emerging as a vital tool in food safety and public health, and its potential to become the gold standard in outbreak detection has been recognized by public health authorities in the USA, the European Union, Australia and China, for example. This review highlights the role of WGS in foodborne outbreak investigations, its implementation challenges, and its impact on public health surveillance.</p>","PeriodicalId":12458,"journal":{"name":"Frontiers in Cellular and Infection Microbiology","volume":"15 ","pages":"1593219"},"PeriodicalIF":4.6,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12066639/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143984474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}