Frontiers in Cellular and Infection Microbiology最新文献

{"title":"Clinical characteristics, molecular epidemiology and mechanisms of colistin heteroresistance in <i>Enterobacter cloacae</i> complex.","authors":"Chunli Wei, Jiming Wu, Jisheng Zhang, Youtao Liang, Kaixin Yu, Mingjing Liao, Xushan Liang, Jianmin Wang, Wenzhang Long, Jin Wang, Shijian Chen, Yang Yang, Xue Gong, Jie Li, Xiaoli Zhang","doi":"10.3389/fcimb.2025.1536058","DOIUrl":"10.3389/fcimb.2025.1536058","url":null,"abstract":"<p><strong>Introduction: </strong>Colistin has emerged as the last resort for treating multidrug-resistant <i>Enterobacter cloacae</i> complex (ECC) infections. The primary purposes of this study were to demonstrate the presence of colistin heteroresistance in ECC and to further investigate their clinical characteristics, molecular epidemiology and mechanisms.</p><p><strong>Methods: </strong>Population analysis profiles (PAP) were performed to confirm the heteroresistance phenotype. Average nucleotide identity (ANI) was determined to classify ECC species. Phylogenetic analysis based on core genome single nucleotide polymorphisms (cg-SNPs), multilocus sequence typing (MLST) and core genome MLST (cg-MLST). Risk factors and clinical outcomes of infections were analyzed through a retrospective case-control study. Potential mechanisms of colistin heteroresistance were evaluated using polymerase chain reaction (PCR), efflux pump inhibition assays and reverse transcription quantitative PCR (RT-qPCR).</p><p><strong>Results: </strong>A high proportion (24.4%) of the non-resistant strains were colistin-heteroresistant isolates. Among the several ECC species, <i>Enterobacter kobei</i> had the largest percentage (29.4%) of colistin-heteroresistant isolates, followed by <i>Enterobacter hormaechei</i> (20.5%) and <i>Enterobacter bugandensis</i> (20.0%). Notably, only one strain (0.8%; 1/132) of <i>Enterobacter hormaechei</i> was fully resistant to colistin. Different ECC species showed varying heteroresistance levels: <i>Enterobacter roggenkampii</i>, <i>Enterobacter kobei</i>, <i>Enterobacter asburiae</i> and <i>Enterobacter bugandensis</i> displayed high heteroresistance levels  (MIC ≥ 128 mg/L). 75% of all ST116 and ST56 strains were heteroresistant to colistin. The infection of ST116 and ST56 strains as well as exposure to cephalosporin antibiotics were independent risk factors for colistin-heteroresistant ECC infections. Mechanistic analysis revealed that heteroresistance strongly correlated with the overexpression of <i>arnA</i>, regulated by the PhoPQ two-component system (TCS). Notably, <i>mgrB</i> had minimal impact. AcrAB-TolC efflux pump genes showed unsynchronized expression; High <i>acrB</i> expression was strongly associated with colistin heteroresistance, while <i>acrA</i> and <i>tolC</i> were not.</p><p><strong>Discussion: </strong>Colistin heteroresistance showed species-dependent variations in levels and prevalence rates. The colistin-heteroresistant mechanisms were complex, involving coordinated regulation of multiple genes. These results highlighted the need for tailored antimicrobial stewardship. In addition, the development of direct, reliable and rapid clinical methods for detecting heteroresistance is essential for improving infection management and prevention.</p>","PeriodicalId":12458,"journal":{"name":"Frontiers in Cellular and Infection Microbiology","volume":"15 ","pages":"1536058"},"PeriodicalIF":4.6,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11922889/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143668957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enterobactin and salmochelin S4 inhibit the growth of <i>Staphylococcus aureus</i>.","authors":"Yaacov Davidov, Noa Tejman-Yarden, Ari Robinson, Galia Rahav, Israel Nissan","doi":"10.3389/fcimb.2025.1456046","DOIUrl":"10.3389/fcimb.2025.1456046","url":null,"abstract":"<p><p>There is increasing demand for novel antimicrobial agents to tackle the antimicrobial resistance crisis. Here we report that two <i>Enterobacteriaceae</i>-produced siderophores, enterobactin and salmochelin S4, inhibit the growth of <i>Staphylococcus aureus</i> isolates, including methicillin-resistance <i>S. aureus</i> (MRSA) clinical isolates. The IC₅₀ for different <i>S. aureus</i> isolates were 2-5 µM for salmochelin S4 and 5-10 µM for enterobactin. This inhibitory activity was partially repressed by adding Fe⁺³. These siderophores also inhibited the growth of <i>Enterococcus</i> strains, including vancomycin-resistant enterococci (VRE) clinical isolates, though less effectively than for <i>S. aureus</i>. The growth of various Gram-negative bacteria was barely affected by these siderophores. These results shed new light on the role of enterobactin and salmochelin in bacterial physiology and ecology and have potential for the development of novel strategies to combat the rapid rise of multidrug-resistant bacteria.</p>","PeriodicalId":12458,"journal":{"name":"Frontiers in Cellular and Infection Microbiology","volume":"15 ","pages":"1456046"},"PeriodicalIF":4.6,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11919883/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143663137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimized oxygen therapy improves sleep deprivation-induced cardiac dysfunction through gut microbiota.","authors":"Shuqi Cai, Zixuan Li, Jie Bai, Yue Ding, Ruisang Liu, Liben Fang, Dengyong Hou, Sheng Zhang, Xiaohui Wang, Yujia Wang, Yuyu Jiang, Yan Xiang, Wenhui Wu, Ying He, Yunkai Zhang, Xiaomeng Ren","doi":"10.3389/fcimb.2025.1522431","DOIUrl":"10.3389/fcimb.2025.1522431","url":null,"abstract":"<p><p>Adequate sleep is of paramount importance for relieving stress and restoring mental vigor. However, the adverse physiological and pathological responses resulting from sleep insufficiency or sleep deprivation (SD) are becoming increasingly prevalent. Currently, the impact of sleep deficiency on gut microbiota and microbiota-associated human diseases, especially cardiac diseases, remains controversial. Here, we employed the following methods: constructed an experimental sleep-deprivation model in mice; conducted 16S rRNA sequencing to investigate the changes in gut microbiota; through fecal microbiota transplantation (FMT) experiments, transplanted fecal microbiota from sleep-deprived mice to other mice; established an environment with a 30% oxygen concentration to explore the therapeutic effects of oxygen therapy on gut microbiota-associated cardiac fibrosis and dysfunction; and utilized transcriptome data to study the underlying mechanisms of oxygen therapy. The results revealed that: sleep-deprived mice exhibited weakness, depression-like behaviors, and dysfunction in multiple organs. Pathogenic cardiac hypertrophy and fibrosis occurred in sleep-deprived mice, accompanied by poor ejection fraction and fractional shortening. 16S rRNA sequencing indicated that sleep deprivation induced pathogenic effects on gut microbiota, and similar phenomena were also observed in mice that received fecal microbiota from sleep-deprived mice in the FMT experiments. The environment with a 30% oxygen concentration effectively alleviated the pathological impacts on cardiac function. Transcriptome data showed that oxygen therapy targeted several hypoxia-dependent pathways and inhibited the production of cardiac collagen. In conclusion, these results demonstrate the significance of sufficient sleep for gut microbiota and may represent a potential therapeutic strategy, where the oxygen environment exerts a protective effect on insomniacs through gut microbiota.</p>","PeriodicalId":12458,"journal":{"name":"Frontiers in Cellular and Infection Microbiology","volume":"15 ","pages":"1522431"},"PeriodicalIF":4.6,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11919660/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143663138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid detection of <i>Mycoplasma pneumoniae</i> CARDS toxin in clinical respiratory specimens by a loop-mediated isothermal amplification assay.","authors":"Yun Fang, Panpan Xie, Xin Zhang, Yue Zhang, Ning Yang, Yinghui Shi, Ruixia Xin, Yunbiao Bai, Wenkai Niu, Xin Yuan","doi":"10.3389/fcimb.2025.1496829","DOIUrl":"10.3389/fcimb.2025.1496829","url":null,"abstract":"<p><p>In light of the absence of rapid and precise diagnostic laboratory tests for the detection of <i>Mycoplasma pneumoniae</i> (MP), a prominent etiological agent implicated in a range of respiratory infections, we developed and evaluated a rapid and straightforward loop-mediated isothermal amplification (LAMP) assay targeting the MP community-acquired respiratory distress syndrome toxin (CARDS TX) gene. The LAMP assay was performed at 65°C for a duration of 60 min, yielding a minimum detection concentration of MP CARDS TX at 0.4986 pg/μl. The assay exhibited no cross-reactivity with 13 other prevalent pathogens associated with respiratory infections or with other common bacterial toxin genes. To further substantiate the validity of the LAMP assay, 200 pharyngeal swabs or bronchoalveolar lavage (BAL) samples were collected from inpatients diagnosed with community-acquired pneumonia (CAP) between June 2021 and July 2022. The results were compared with those obtained by the quantitative real-time polymerase chain reaction (qPCR) method for verification purposes. Of the 200 clinical specimens, 11 exhibited positive results for MP by LAMP and 10 displayed positive results for MP by qPCR (<i>P</i> = 1.000). In summary, a sensitive, specific, straightforward, and expeditious LAMP method for CARDS TX identification was developed to facilitate rapid detection of MP in point-of-care settings. This assay enables early and accurate diagnosis, even in resource-limited environments, which is important for proper antibiotic treatment and prognosis of MP infection.</p>","PeriodicalId":12458,"journal":{"name":"Frontiers in Cellular and Infection Microbiology","volume":"15 ","pages":"1496829"},"PeriodicalIF":4.6,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11962300/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143771926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigating the Gut Microbiota Profile in Prehypertensive Individuals Exhibiting Phlegm-Dampness Constitution.","authors":"Ning Yu, Yaotang Yang, Guangyun Wang, Yanhong Wang, Mei Feng, Peilin Yang, Shuang Liu, Rui-Rui Wang, Lei Zhang","doi":"10.3389/fcimb.2025.1507076","DOIUrl":"10.3389/fcimb.2025.1507076","url":null,"abstract":"<p><strong>Background: </strong>Prehypertension is the preclinical stage of hypertension, which is more likely to develop into hypertension than normal blood pressure. Although the body may experience pathological changes at this stage, there are often no symptoms. Chinese medicine constitution theory is widely used to assess an individual's health and disease status, which provides a new method for disease prevention. The phlegm-dampness constitution (PDC) is the main constitution in prehypertension. Dysbiosis of the intestinal flora is considered to be related to the development of hypertension. However, the characteristics of the intestinal flora in prehypertensive populations with PDCs are still unknown.</p><p><strong>Methods: </strong>16S rRNA gene sequencing of fecal samples was performed in this study, which included 30 prehypertensive subjects with PDCs, 30 nonphlegm-dampness constitution (NPDC) prehypertensive individuals with balanced constitution, and 30 ideal blood pressure subjects with balanced constitution (BC). On the basis of the composition of the intestinal flora, a random forest classifier was constructed to screen the specific bacteria of the prehypertensive PDC population, and the diagnostic efficiency was determined by the area under the curve (AUC).</p><p><strong>Results: </strong>At the phylum level, the abundance of <i>Bacteroidetes</i> decreased in the PDC group compared with the NPDC group. <i>Bacteroides</i> was the most important genus at the genus level. Compared with those in the NPDC or BC group, the relative abundances of <i>o_RF39</i>, <i>f_Porphyromonadaceae</i>, <i>f_Christensenellaceae</i>, <i>g_parabacteroides</i>, and <i>g_nitrobacteria</i> in the PDC group were significantly greater. The random forest analysis results revealed that <i>Alistipes</i>, <i>Butyricimonas</i>, <i>Odoribacter</i>, <i>Parabacteroides</i>, and <i>Corynebacterium</i> are bacterial genera that significantly differ between the PDC and NPDC groups and greatly contribute to group differentiation. Receiver operating characteristic (ROC) analysis revealed that the AUC range of differential bacteria and its combined diagnostic model ranged from 0.653 (95% CI: 0.511-0.794) to 0.706 (95% CI: 0.573-0.838), suggesting that it is a potential risk marker for phlegm-dampness constitution with prehypertension.</p><p><strong>Conclusions: </strong>Our study indicates that PDC individuals with prehypertension can be distinguished from NPDC individuals according to their gut microbiome characteristics. Prevention and treatment measures based on these biomarkers may be beneficial in opening new ideas and directions for identifying more aggressive and effective interventions for prehypertensive populations.</p>","PeriodicalId":12458,"journal":{"name":"Frontiers in Cellular and Infection Microbiology","volume":"15 ","pages":"1507076"},"PeriodicalIF":4.6,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11913815/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143656838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Editorial: Global excellence in fungal pathogenesis: Asia & Australasia.","authors":"Shweta Panchal, Zhangyong Si","doi":"10.3389/fcimb.2025.1573927","DOIUrl":"10.3389/fcimb.2025.1573927","url":null,"abstract":"","PeriodicalId":12458,"journal":{"name":"Frontiers in Cellular and Infection Microbiology","volume":"15 ","pages":"1573927"},"PeriodicalIF":4.6,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11913673/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143656837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Significance of the gut tract in the therapeutic mechanisms of polydopamine for acute cerebral infarction: neuro-immune interaction through the gut-brain axis.","authors":"Feng-Hua Xu, Xiao Sun, Jun Zhu, Ling-Yang Kong, Yuan Chang, Ning Li, Wen-Xiang Hui, Cong-Peng Zhang, Yi-Ming Cheng, Wen-Xin Han, Zhi-Min Tian, Yan-Ning Qiao, Dong-Feng Chen, Lei Liu, Da-Yun Feng, Jing Han","doi":"10.3389/fcimb.2024.1413018","DOIUrl":"10.3389/fcimb.2024.1413018","url":null,"abstract":"<p><strong>Background: </strong>Recent research has made significant progress in elucidating gastrointestinal complications following acute cerebral infarction (ACI), which includes disorders in intestinal motility and dysbiosis of the gut microbiota. Nevertheless, the role of the gut (which is acknowledged as being the largest immune organ) in the immunoreactive effects of polydopamine nanoparticles (PDA) on acute ischemic stroke remains inadequately understood. In addition to its function in nutrient absorption, the gut acts as a protective barrier against microbes. Systemic immune responses, which are triggered by the disruption of gut barrier integrity, are considered as one of the mechanisms underlying acute ischemic stroke, with the gut-brain axis (GBA) playing a pivotal role in this process.</p><p><strong>Methods: </strong>In this study, we used a PDA intervention in an ACI model to investigate ACI-like behavior, intestinal barrier function, central and peripheral inflammation, and hippocampal neuron excitability, thus aiming to elucidate the mechanisms through which PDA improves ACI via the GBA.</p><p><strong>Results: </strong>Our findings indicated that as ACI mice experienced dysbiosis of the gut microbiota and intestinal barrier damage, the levels of proinflammatory factors in the serum and brain significantly increased. Additionally, the activation of astrocytes in the hippocampal region and neuronal apoptosis were observed in ACI mice. Importantly, our study is the first to provide evidence demonstrating that PDA effectively suppresses the neuroimmune interactions of the gut-brain axis and significantly improves intestinal epithelial barrier integrity.</p><p><strong>Conclusion: </strong>We hope that our discoveries will serve as a foundation for further explorations of the therapeutic mechanisms of PDA in ACI, particularly in elucidating the protective roles of gut microbiota and intestinal barrier function, as well as in the development of more targeted clinical interventions for ACI.</p>","PeriodicalId":12458,"journal":{"name":"Frontiers in Cellular and Infection Microbiology","volume":"14 ","pages":"1413018"},"PeriodicalIF":4.6,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11913817/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143656836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Macrolide resistance in <i>Mycoplasma pneumoniae</i> in adult patients.","authors":"Panpan Xie, Yue Zhang, Yanhong Qin, Yun Fang, Ning Yang, Yunbiao Bai, Shimeng Zhi, Wenkai Niu, Fusheng Wang, Xin Yuan","doi":"10.3389/fcimb.2025.1496521","DOIUrl":"10.3389/fcimb.2025.1496521","url":null,"abstract":"<p><p><i>Mycoplasma pneumoniae</i> is one of the most significant pathogens responsible for respiratory infections in humans. Macrolides are recommended as the first-line treatment for <i>M. pneumoniae</i> infection. The prevalence of macrolide-resistant <i>M. pneumoniae</i> has increased significantly in recent decades, particularly in China. The mechanisms of resistance in <i>M. pneumoniae</i> to macrolides have been extensively studied in pediatric patients. However, a paucity reports regarding the resistance characteristics and mechanisms exhibited in adults. The aim of this study was to elucidate the resistance of <i>M. pneumoniae</i> to macrolides and the underlying mechanisms in adult patients. Pharyngeal swab specimens were collected from adult patients presenting with subacute cough or community-acquired pneumonia at our hospital from January 2011 to June 2017 to identify and isolate <i>M. pneumoniae</i> strains. The antimicrobial susceptibility of these isolates to 3 macrolide antibiotics was assessed using broth microdilution method. The <i>23S rRNA</i> genes of macrolide-resistant <i>M. pneumoniae</i> strains were sequenced, and the presence of target methylation genes (<i>ermA</i>, <i>ermB</i>, and <i>ermC</i>), efflux pump genes (<i>mefA</i>, <i>mefA/E</i>, <i>msrA</i>, and <i>msrA/B</i>), and the macrolide resistance gene <i>mphC</i> was identified through polymerase chain reaction (PCR) testing. Additionally, MICs were determined with and without the efflux pump inhibitor reserpine. A total of 72 <i>M. pneumoniae</i> strains were isolated from adult patients, with 41.7% (30/72) exhibiting macrolide resistance. Among the 3 macrolides tested, the 16-membered-ring midecamycin exhibited the greatest activity (MIC₉₀: 16 µg/ml) against <i>M. pneumoniae</i>. All macrolide-resistant <i>M. pneumoniae</i> strains harbored mutations at the 2063 site in domain V of the <i>23S rRNA</i> gene. Two macrolide-resistant <i>M. pneumoniae</i> clinical isolates were found to harbor the efflux pump genes <i>msrA/B</i> and <i>mefA</i>. The efflux pump inhibitor reserpine reduced the MIC for azithromycin in these two strains to a quarter of their original values. In summary, macrolide-resistant <i>M. pneumoniae</i> is commonly observed among adults in Beijing. Point mutations are the primary mechanism responsible for macrolide resistance in adults with <i>M. pneumoniae</i>. Additionally, the efflux pump mechanism may contribute partially to this resistance. Midecamycin presents a promising alternative drug for treating <i>M. pneumoniae</i> infections, particularly in cases of azithromycin-resistant <i>M. pneumoniae</i> infection in young children.</p>","PeriodicalId":12458,"journal":{"name":"Frontiers in Cellular and Infection Microbiology","volume":"15 ","pages":"1496521"},"PeriodicalIF":4.6,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11955812/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143751729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First environmental survey of <i>Scedosporium</i> species in Lebanon.","authors":"Sara Mina, Hajar Yaakoub, Bienvenue Razafimandimby, Elske Dwars, Méline Wéry, Nicolas Papon, Wieland Meyer, Jean-Philippe Bouchara","doi":"10.3389/fcimb.2025.1547800","DOIUrl":"10.3389/fcimb.2025.1547800","url":null,"abstract":"<p><strong>Background: </strong><i>Scedosporium</i> species are filamentous fungi causing a wide spectrum of infections in healthy and debilitated individuals. Despite their clinical significance, the ecology of <i>Scedosporium</i> species remains understudied, particularly in the Middle East.</p><p><strong>Methods: </strong>In this context, we conducted an environmental study to elucidate the distribution and ecological preferences of <i>Scedosporium</i> species in the North of Lebanon. One hundred and fifty-five soil samples were collected from different environmental areas and analyzed for several chemical parameters. <i>Scedosporium</i> isolates were then selected for species identification and genotyping.</p><p><strong>Results: </strong>Overall, 39 (25.16%) were positive for <i>Scedosporium</i> species, with a predominance of <i>S. apiospermum</i> (80.56%). Soil analysis revealed associations between the fungal presence and pH, nitrogen, phosphorus, and organic matter content. Moreover, genotyping analysis using MultiLocus Sequence Typing identified five major clusters. Interestingly, a number of Lebanese isolates formed an Asian-specific cluster (V) with one clinical Chinese isolate, whereas two clusters (II and III) showed a close association with German isolates, and clusters (I and IV) contained isolates with a global distribution.</p><p><strong>Conclusion: </strong>These findings provide new insights into the ecology of <i>Scedosporium</i> species, bridging a gap in our knowledge of their distribution on the Asian continent and laying the groundwork for future clinical investigations. Future international collaborations are essential to trace the origin of <i>S. apiospermum</i>.</p>","PeriodicalId":12458,"journal":{"name":"Frontiers in Cellular and Infection Microbiology","volume":"15 ","pages":"1547800"},"PeriodicalIF":4.6,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11911385/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143648048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Targeting CD4+ T cells through gut microbiota: therapeutic potential of traditional Chinese medicine in inflammatory bowel disease.","authors":"Xingyao Lu, Yichuan Xv, Weiye Hu, Boyun Sun, Hongyi Hu","doi":"10.3389/fcimb.2025.1557331","DOIUrl":"10.3389/fcimb.2025.1557331","url":null,"abstract":"<p><p>Inflammatory Bowel Disease (IBD) is an autoimmune disease characterized by chronic relapsing inflammation of the intestinal tract. Gut microbiota (GM) and CD4⁺T cells are important in the development of IBD. A lot of studies have shown that GM and their metabolites like short-chain fatty acids, bile acids and tryptophan can be involved in the differentiation of CD4⁺T cells through various mechanisms, which in turn regulate the immune homeostasis of the IBD patients. Therefore, regulating CD4⁺T cells through GM may be a potential therapeutic direction for the treatment of IBD. Many studies have shown that Traditional Chinese Medicine (TCM) formulas and some herbal extracts can affect CD4⁺T cell differentiation by regulating GM and its metabolites. In this review, we mainly focus on the role of GM and their metabolites in regulating the differentiation of CD4⁺T cells and their correlation with IBD. We also summarize the current research progress on the regulation of this process by TCM.</p>","PeriodicalId":12458,"journal":{"name":"Frontiers in Cellular and Infection Microbiology","volume":"15 ","pages":"1557331"},"PeriodicalIF":4.6,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11911530/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143646845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}