Frontiers in Cellular and Infection Microbiology最新文献

{"title":"Editorial: New techniques in microbiome research - volume II: Host-microbiome interactions using 'meta-omics' techniques.","authors":"Tao Lin","doi":"10.3389/fcimb.2025.1541881","DOIUrl":"10.3389/fcimb.2025.1541881","url":null,"abstract":"","PeriodicalId":12458,"journal":{"name":"Frontiers in Cellular and Infection Microbiology","volume":"15 ","pages":"1541881"},"PeriodicalIF":4.6,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11843044/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143482609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gut microbiota interacting with vitamin D but not anandamide might contribute to the pathogenesis of preeclampsia: a preliminary study.","authors":"Xiao-Qiang Han, Hui-Hui Jiang, Meng-Ling Chen, De-Yang Han, Su-Fen Zhou, Jin-Wen Wang, Shu-Shen Ji, Ling-Yun Wang, Jing-Wei Lou, Ming-Qun Li","doi":"10.3389/fcimb.2024.1469054","DOIUrl":"10.3389/fcimb.2024.1469054","url":null,"abstract":"<p><strong>Introduction: </strong>Preeclampsia (PE) is a pregnancy-specific multisystem disorder and a leading cause of maternal and perinatal mortality globally. Despite numerous studies highlighting the potential roles of gut microbiota, anandamide (AEA), and Vitamin D (VitD) in PE, none have established them as reliable biomarkers for predicting disease onset. Moreover, their interactions in late-stage pregnancy women remain poorly understood.</p><p><strong>Methods: </strong>Thirty-four preeclamptic patients (called PE group) and thirty-nine matched healthy late-pregnant women (called LP group) were involved in this case-control study. Fecal samples, which were used to acquire the diversity and composition of gut microbiota, were analyzed by 16S rRNA gene sequencing. Plasma AEA concentrations and serum VitD levels were determined by high-performance liquid chromatography-mass spectrometry (HPLC-MS) and liquid chromatography-tandem mass spectrometry (LC-MS/MS), respectively.</p><p><strong>Results: </strong>In this study, β diversity but not α diversity significantly differed between the LP and PE groups. Compared with the LP group, the relative abundances of <i>Prevotella</i>, <i>Erysipelotrichaceae_UCG-003</i>, and <i>Dorea</i> were increased dramatically in the PE group, whereas the relative abundances of <i>Subdoligranulum</i>, <i>Parabacteroides</i>, <i>Bacteroides</i> were significantly decreased in the PE group. Furthermore, women with PE had a substantially lower plasma level of AEA and a marked decrease in serum VitD compared to normal late-pregnant women. Lastly, although the serum level of AEA was not significantly correlated with VitD or any of the top 6 marker genera, VitD was significantly negatively correlated with the relative abundance of <i>Dorea</i>, a novel finding in this context.</p><p><strong>Discussion: </strong>The gut microbiota profile of the PE group was significantly different from that of the LP group. Although no significant correlations were identified between the plasma AEA levels and serum VitD levels or any of the top 6 identified marker genera, a significant negative correlation was observed between VitD and Dorea, indicating VitD and gut microbiota have the potential to be combined targets for early diagnosis and management of PE.</p>","PeriodicalId":12458,"journal":{"name":"Frontiers in Cellular and Infection Microbiology","volume":"14 ","pages":"1469054"},"PeriodicalIF":4.6,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11835824/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143457505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A short ncRNA modulates gene expression and affects stress response and parasite differentiation in <i>Leishmania braziliensis</i>.","authors":"José C Quilles, Caroline R Espada, Lissur A Orsine, Tânia A Defina, Letícia Almeida, Fabíola Holetz, Angela K Cruz","doi":"10.3389/fcimb.2025.1513908","DOIUrl":"10.3389/fcimb.2025.1513908","url":null,"abstract":"<p><p>The protozoan parasite <i>Leishmania</i> spp. is a causative agent of leishmaniasis, a disease that affects millions of people in more than 80 countries worldwide. Apart from its medical relevance, this organism has a genetic organization that is unique among eukaryotes. Studies of the mechanisms regulating gene expression in <i>Leishmania</i> led us to investigate noncoding RNAs (ncRNAs) as regulatory elements. We previously identified differentially expressed (DE) ncRNAs in <i>Leishmania braziliensis</i> with potential roles in the parasite biology and development. Herein, we present a functional analysis of one such DE ncRNA, the 147-nucleotide-long transcript ncRNA97, which is preferentially expressed in amastigotes, the replicative form within mammalian phagocytes. By RT-qPCR the ncRNA97 was detected in greater quantities in the nucleus under physiological conditions and in the cytoplasm under nutritional stress. Interestingly, the transcript is protected at the 5' end but is not processed by the canonical trypanosomatid <i>trans</i>-splicing mechanism, according to the RNA circularization assay. ncRNA97 knockout (^KO) and addback (^AB) transfectants were generated and subjected to phenotypic analysis, which revealed that the lack of ncRNA97 impairs the starvation response and differentiation to the infective form. Comparative transcriptomics of ncRNA97^KO and parental cells revealed that transcripts encoding amastigote-specific proteins were affected. This pioneering work demonstrates that ncRNAs contribute to the developmental regulatory mechanisms of <i>Leishmania</i>.</p>","PeriodicalId":12458,"journal":{"name":"Frontiers in Cellular and Infection Microbiology","volume":"15 ","pages":"1513908"},"PeriodicalIF":4.6,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11841412/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143467608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diverse vaginal microbiota in healthy Japanese women: a combined relative and quantitative analyses.","authors":"Masahiro Ito, Misaki Kataoka, Yoichi Sato, Hideki Nachi, Koji Nomoto, Nobuhiko Okada","doi":"10.3389/fcimb.2024.1487990","DOIUrl":"10.3389/fcimb.2024.1487990","url":null,"abstract":"<p><strong>Introduction: </strong>This cross-sectional study aimed to characterize the viable vaginal microbiota and identify host factors influencing this microbiota by employing a combination of relative and quantitative analyses.</p><p><strong>Methods: </strong>Twenty-four vaginal fluid samples were collected from healthy adult Japanese women for analysis. Vaginal fluid pH was measured using a portable pH meter. DNA was extracted from the vaginal fluid, and the 16S ribosomal RNA gene sequences in the V3-V4 regions were analyzed to identify bacterial species. Additionally, the vaginal fluid was cultured on four types of selective agar plates. The predominant species in the growing colonies were identified using colony polymerase chain reaction, and the colonies were counted.</p><p><strong>Results: </strong>The vaginal microbiota was classified into four categories based on the characterization of the dominant bacterial population: <i>Lactobacillus crispatus</i>, <i>Lactobacillus iners</i>, <i>Lactobacillus gasseri</i>, and a diversity group. The predominant bacterial species were consistent across methods; however, the levels of the viable population varied significantly. Body mass index had a significant influence on the total number of viable bacteria and vaginal pH, while age only affected vaginal pH.</p><p><strong>Conclusions: </strong>Our findings indicate that the vaginal microbiome of healthy Japanese women is not only highly diverse but also affected by host factors such as BMI and age.</p>","PeriodicalId":12458,"journal":{"name":"Frontiers in Cellular and Infection Microbiology","volume":"14 ","pages":"1487990"},"PeriodicalIF":4.6,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11832463/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143448662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Roles of the <i>rpoEc-chrR-chrA</i> operon in superoxide tolerance and β-lactam susceptibility of <i>Stenotrophomonas maltophilia</i>.","authors":"Ren-Hsuan Ku, Hsu-Feng Lu, Li-Hua Li, Ting-Yu Yeh, Yi-Tsung Lin, Tsuey-Ching Yang","doi":"10.3389/fcimb.2025.1492008","DOIUrl":"10.3389/fcimb.2025.1492008","url":null,"abstract":"<p><strong>Introduction: </strong>The <i>rpoE-chrR</i> pair is a regulatory system used by photosynthetic microorganisms to overcome singlet oxygen stress. <i>rpoE</i> and <i>chrR</i> encode the sigma factor σ^E and anti-sigma factor ChrR, respectively. Stenotrophomonas maltophilia, an opportunistic pathogen, is a multidrug-resistant gram-negative bacterium. Although it is not a photosynthetic microorganism, a <i>rpoE-chrR</i> homolog (<i>smlt2377-smlt2378</i>) was found in the <i>S. maltophilia</i> genome. In this study, we aimed to assess the significance of σ^Ec-ChrR pair in oxidative stress alleviation and antibiotic susceptibility of <i>S. maltophilia</i> KJ.</p><p><strong>Methods: </strong>Reverse transcription-polymerase chain reaction was used to validate the presence of operon. The contribution of <i>rpoEc-chrR-chrA</i> operon to oxidative stress alleviation and antibiotic susceptibility was evaluated using mutant constructs and stress-tolerance assays. RNA-seq transcriptome assay of wild-type KJ, KJΔChrR (<i>chrR</i> mutant), and KJΔChrRΔRpoEc (<i>chrR/rpoEc</i> double mutant) was performed to reveal the σ^Ec regulon.</p><p><strong>Results: </strong>The <i>rpoEc-chrR</i> pair and downstream chrA formed an operon. Inactivation of <i>chrR</i> upregulated the expression of <i>rpoEc-chrR-chrA</i> operon in an σ^Ec- and ChrA-dependent manner. σ^Ec activation contributed to superoxide tolerance and increased β-lactam susceptibility but did not affect the tolerance to singlet oxygen and hydrogen peroxide. Transcriptome analysis revealed that expression of the nine-gene cluster, <i>smlt2375-smlt2367</i>, was significantly upregulated in KJΔChrR and reverted to the wild-type level in KJΔChrRΔRpoEc. <i>smlt2375-smlt2367</i> cluster was located upstream of the <i>rpoEc-chrR-chrA</i> operon and divergently transcribed, seeming to be involved in membrane lipid modification. Deletion of <i>smlt2375-smlt2367</i> cluster from the chromosome of KJΔChrR reverted the superoxide tolerance and β-lactam susceptibility to the wild-type level.</p><p><strong>Discussion: </strong>The <i>rpoEc-chrR</i> pair of <i>S. maltophilia</i> was involved in superoxide tolerance and β-lactam susceptibility. Notably, a novel regulatory circuit involving <i>rpoEc-chrR-chrA</i> operon and <i>smlt2375-smlt2367</i> cluster was revealed.</p>","PeriodicalId":12458,"journal":{"name":"Frontiers in Cellular and Infection Microbiology","volume":"15 ","pages":"1492008"},"PeriodicalIF":4.6,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11832516/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143448723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Species evolution: cryptic species and phenotypic noise with a particular focus on fungal systematics.","authors":"Anusha H Ekanayaka, Samantha C Karunarathna, Saowaluck Tibpromma, Arun Kumar Dutta, Danushka Sandaruwan Tennakoon, Anuruddha Karunarathna, Ekachai Chukeatirote, Dong-Qin Dai, Steven L Stephenson, Sajeewa S N Maharachchikumbura, Chao Liu, Alan J L Phillips","doi":"10.3389/fcimb.2025.1497085","DOIUrl":"10.3389/fcimb.2025.1497085","url":null,"abstract":"<p><p>The evolution of a species can be understood in the context of two major concepts-the cryptic species concept and the phenotypic noise concept. The former represents morphologically indistinguishable but genetically distinct evolutionary lineages, while the latter represents the phenotypic variations of an isogenic population. Although the concept of cryptic species currently represents a general topic, its effect on other aspects of biology, such as biodiversity, ecology, evolutionary biology, and taxonomy, is still unclear. In particular, cryptic species cause complications and prevent the development of a clear taxonomy. The phenotypic noise concept or phenotypic plasticity generally refers to the various expressions of phenotypes in different environments. Hence, the cryptic species concept refers to genetic variations, while the phenotypic noises concept is about non-genetic variations. Although both concepts are opposites, they each contribute significantly to the evolutionary process of an organism. Despite the extensive research studies and publications discussing those two concepts in separate accounts, a concise account that combines and compares both concepts are generally lacking. Nevertheless, these are essential to understand the evolutionary process clearly. This review addresses the available literature on this topic, intending to provide a general and overall discussion on both the cryptic species concept and the phenotypic noise concept and their effect on evolution, ecology, biodiversity, and taxonomy with a special focus on fungal systematics. hence, several fungal case studies representing the two concepts are presented, compared, and discussed for a better understanding.</p>","PeriodicalId":12458,"journal":{"name":"Frontiers in Cellular and Infection Microbiology","volume":"15 ","pages":"1497085"},"PeriodicalIF":4.6,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11832716/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143448739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Editorial: Metabolomics in bacterial infections.","authors":"Vijay Soni, Aditya Upadhyay, Yong Zhang","doi":"10.3389/fcimb.2025.1557057","DOIUrl":"https://doi.org/10.3389/fcimb.2025.1557057","url":null,"abstract":"","PeriodicalId":12458,"journal":{"name":"Frontiers in Cellular and Infection Microbiology","volume":"15 ","pages":"1557057"},"PeriodicalIF":4.6,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11832537/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143448762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Overcoming beta-lactam resistance in <i>Pseudomonas aeruginosa</i> by targeting metallo-beta-lactamase VIM-1: a one-microsecond molecular dynamics simulation study.","authors":"Mohammed Salleh M Ardawi, Samar A Badreddine, Muhammad Yasir, Aiah M Khateb, Safaa A Turkistani, Ahmed Afandi, Samah O Noor, Adhari Alselmi, Vivek Dhar Dwivedi, Esam I Azhar","doi":"10.3389/fcimb.2025.1521391","DOIUrl":"10.3389/fcimb.2025.1521391","url":null,"abstract":"<p><p><i>Pseudomonas aeruginosa (P. aeruginosa)</i> is a Gram-negative opportunistic pathogen with a high resistance to beta-lactam antibiotics, mainly due to the production of metallo-beta-lactamase VIM-1 (MBL-VIM-1) enzyme. This study aimed to identify new inhibitors targeting MBL-VIM-1 to restore the efficacy of beta-lactam antibiotics. Extensive screening of natural compounds from the COCONUT database was performed to identify the structural analogs of the existing inhibitor of the MBL-VIM-1 protein. The virtual screening process selected four top-performing compounds (CNP0390322, CNP03905695, CNP0079056, and CNP0338283) that exhibited promising docking scores. These compounds were then subjected to re-docking and one-microsecond molecular dynamics (MD) simulations to assess their binding stability and interactions within the MBL-VIM-1 active site. Finally, post-MD simulation calculations were employed to estimate the interaction strengths and compare the efficacy of these compounds against the reference inhibitor. The findings highlighted that these four potent MBL-VIM-1 inhibitors show superior binding affinity and stability, suggesting their potential to combat antibiotic resistance in <i>P. aeruginosa</i>. The identified compounds offer a promising avenue for developing novel therapeutics to restore the efficacy of beta-lactam antibiotics against resistant bacterial strains. Therefore, further <i>in vitro</i> and <i>in vivo</i> studies are warranted to validate their potential.</p>","PeriodicalId":12458,"journal":{"name":"Frontiers in Cellular and Infection Microbiology","volume":"15 ","pages":"1521391"},"PeriodicalIF":4.6,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11832520/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143448704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Knowledge, attitude, and perception of Pakistani populations toward monkeypox: a cross-section study.","authors":"Humayun Yousaf, Abdul Qadeer, Muhammad Sohail, Maqbool Khan, Muhammad Farooq, Zakir Khan, Dalia Fouad, Yu-Chia Liu, Chien-Chin Chen","doi":"10.3389/fcimb.2024.1449096","DOIUrl":"10.3389/fcimb.2024.1449096","url":null,"abstract":"<p><strong>Objective: </strong>The reappearance of monkeypox in non-endemic countries has preceded critical public health concerns. The public's adherence to preventative measures is influenced by their understanding of the infectious monkeypox virus (Mpox), attitude toward it, and perceptions of it. We conducted this study to evaluate the general population's knowledge, attitude, and perceptions of Mpox in Pakistan.</p><p><strong>Methods: </strong>From August 15 to August 30, 2022, 3465 participants in this cross-sectional study provided information via an online survey. The survey has 37 questions divided into four categories: sociodemographic, knowledge, attitude, and perceptions of Mpox. Statistical analyses were performed in Jupyter Notebook using Python 3 and the Pandas, Matplotlib, and stats libraries.</p><p><strong>Results: </strong>The chi-square test and regression analysis evaluated factors related to Mpox knowledge, attitude, and perception of three thousand four hundred sixty-five participants. Among the participants, about 79.51% (2755) were male, and 32.99% (1143) had post-graduation level education. About 521(99.24) participants with post-graduation education have positive knowledge with a p-value of 0.0001. Approximately 66.78% (2314) of participants answered that Mpox is prevalent in African countries. Almost 85.69% (2969) stated that a virus causes Mpox, and more than 72.18% (2501) said that Mpox spreads through contaminated surfaces. About 60.52% (2097) of participants answered that antivirals are required in treating Mpox patients. Around more than half of the participants, 52.64% (1824), believe that those who receive the chickenpox vaccination are protected against Mpox. It's interesting to note that most research participants felt favorably about Mpox. Around 90.22% (3126) of participants believed Mpox would be effectively eradicated. Most participants, 86.7% (3004), believe that people should care more about one another nowadays, and 81.36% (2819) believe visiting areas with a Mpox outbreak is risky. About 83.95% (2909) answered that during interaction with the Mpox patient, they'd dress in the appropriate personal protective clothing, like masks, gloves, and gowns. At the same time, 77.66% (2691) responded that they would perform hand hygiene after touching the patients' surroundings, like beds, tables, doors, etc.</p><p><strong>Conclusions: </strong>Maximum research participants showed good attitudes and knowledge about Mpox. However, it is essential to start and execute a planned planning framework for public health awareness to avoid the occurrence and spread of Mpox in Pakistan.</p>","PeriodicalId":12458,"journal":{"name":"Frontiers in Cellular and Infection Microbiology","volume":"14 ","pages":"1449096"},"PeriodicalIF":4.6,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11832577/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143448756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Can fungal endophytes suppress <i>Trialeurodes vaporariorum</i> and the transmission of tomato infectious chlorosis and chlorosis viruses in field conditions?","authors":"Marial Makur Zechariah Paweer, Everlyne Samita Namikoye, Shem Bonuke Nchore, Komivi Senyo Akutse","doi":"10.3389/fcimb.2025.1470821","DOIUrl":"10.3389/fcimb.2025.1470821","url":null,"abstract":"<p><p>Field trials were conducted for two seasons in two experimental sites (Mwea in Kirinyaga and Ngoliba in Kiambu counties of Kenya) to assess the efficacy of fungal endophytes <i>Hypocrea lixii</i> F3ST1 and <i>Trichoderma asperellum</i> M2RT4 in the control of <i>Trialeurodes vaporariorum</i> vector of tomato infectious chlorosis virus (TICV) and tomato chlorosis virus (ToCV) through seeds inoculation. TICV and ToCV's disease incidence, severity and the yield were also evaluated. All the fungal endophytes successfully colonized all the tomato plant parts, but the highest root colonization was observed in <i>H. lixii</i> F3ST1 compared to the <i>T. asperellum</i> M2RT4 in both seasons. The number of nymphs was significantly lower in the endophytically colonized tomato plants than the control treatments in all the seasons and at both sites. However, the lowest number of nymphs was recorded in <i>H. lixii</i> F3ST1 compared to <i>T. asperellum</i> M2RT4. On the other hand, the TICV and ToCV disease incidence and severity rates were lower in endophytically colonized tomato crops compared to the control plots. This could be attributed to the reduction in the virus replication and lower feeding ability of <i>T. vaporariorum</i> that was characterized by less excretion of honeydew causing sooty mold. However, no significant difference was observed in ToCV disease severity rates among the treatments and across the seasons. The yield was significantly higher in endophyte plots than the control treatments in both sites and across the two seasons. This study demonstrates that <i>H. lixii</i> F3ST1 and <i>T. asperellum</i> M2RT4 endophytically colonized tomato plants and conferred systemic resistance against <i>T. vaporariorum</i> vector, and significantly reduced the transmission of TICV and ToCV, contributing to high reduction of both diseases' incidence and severity in the field. However, further studies are warranted to confirm these results at large scale trials.</p>","PeriodicalId":12458,"journal":{"name":"Frontiers in Cellular and Infection Microbiology","volume":"15 ","pages":"1470821"},"PeriodicalIF":4.6,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11832475/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143448759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}