Frontiers in Cellular and Infection Microbiology最新文献

{"title":"Immunoassay-mass spectrometry to identify <i>Brucella melitensis</i>.","authors":"Amirreza Sharif, Ramin Bagheri Nejad, Alireza Ghassempour","doi":"10.3389/fcimb.2025.1531018","DOIUrl":"10.3389/fcimb.2025.1531018","url":null,"abstract":"<p><p>Two factors frequently impede accurate bacterial identification using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS): inadequate bacterial abundance in real samples and bacterial combinations. For MALDI-TOF MS analysis and libraries for bacterial identification, time-consuming culture procedures are necessary to achieve sufficient concentration and isolation of a single bacterium. When dealing with hazardous bacteria like <i>Brucella</i>, which are more difficult to handle and cure, this problem becomes even more crucial. To overcome these obstacles, Fe₃O₄ magnetic nanoparticles (MNPs) linked with <i>Brucella</i>-specific antibodies and MALDI-TOF MS analysis have been used to create a quick and accurate technique for direct bacterial separation and identification in complex samples. This method allows MNPs to immune-selectively collect <i>Brucella</i> cells, which are then deactivated and ready for MALDI-TOF MS analysis by a formic acid/acetonitrile wash. Rabbits were used to manufacture brucella antibodies, which have effectively adsorbed onto the MNPs-protein A. Any particular <i>Brucella</i> bacteria found in the media might be absorbed by this MNPs-protein A-antibody immunoprobe. The concentration of <i>Brucella</i> bacterial cells increases the protein spectrum's visibility by a factor of 10³, making it possible to quickly identify <i>Brucella</i> spp. without first growing them in cultural conditions. This method has been successfully used to achieve a limit of detection (LOD) of 50 CFU/mL in an aqueous medium and genuine sample-milk. The diagnostic time for this harmful bacterium is greatly decreased because the entire procedure from bacterial isolation to species identification is finished in less than 60 min. High sensitivity and specificity are demonstrated by the immunoassay-MS approach, as the spectral pattern it produces matches well-known databases like SPECLUST and Ribopeaks.</p>","PeriodicalId":12458,"journal":{"name":"Frontiers in Cellular and Infection Microbiology","volume":"15 ","pages":"1531018"},"PeriodicalIF":4.6,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11832529/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143448698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the associations of gut microbiota with inflammatory and the early hematoma expansion in intracerebral hemorrhage: from change to potential therapeutic objectives.","authors":"Haixiao Jiang, Wei Zeng, Fei Zhu, Xiaoli Zhang, Demao Cao, Aijun Peng, Hongsheng Wang","doi":"10.3389/fcimb.2025.1462562","DOIUrl":"10.3389/fcimb.2025.1462562","url":null,"abstract":"<p><strong>Background: </strong>Although a great deal of research has explored the possibility of a systemic inflammatory response and dysbiosis of the gut microbiota after an intracerebral hemorrhage (ICH), the relationships between gut microbiota and blood inflammatory indicators as well as their role in the hematoma expansion following an early-stage mild-to-moderate ICH (emICH) remain unknown. This study analyzes these changes and associations in order to predict and prevent hematoma expansion after emICH.</p><p><strong>Methods: </strong>The study included 100 participants, with 70 individuals diagnosed with emICH (30 with hematoma expansion and 40 without hematoma expansion, referred to as the HE and NE groups) and 30 healthy controls matched in terms of age and gender (HC). We used 16S rRNA gene sequencing to explore the gut microbial structure and its underlying associations with blood inflammatory parameters in the HE group.</p><p><strong>Results: </strong>Our findings showed a significant decrease in the diversity and even distribution of microorganisms in the HE group when compared to the HC and NE groups. The composition of the gut microbiota experienced notable alterations in the emICH group, especially in HE. These changes included a rise in the number of gram-negative pro-inflammatory bacteria and a decline in the level of probiotics. Furthermore, we observed strong positive connections between bacteria enriched in the HE group and levels of systemic inflammation. Several microbial biomarkers (e.g. <i>Escherichia_Shigella, Enterobacter</i>, and <i>Porphyromonas</i>) were revealed in disparateiating HE from HC and NE. Analysis of the Kyoto Encyclopedia of Genes and Genomes (KEGG) exposed disturbances in essential physiological pathways, especially those related to inflammation (such as the Toll-like receptor signaling pathway), in the HE group.</p><p><strong>Conclusions: </strong>Our exploration indicated that individuals with emICH, especially those with HE, demonstrate notably different host-microbe interactions when compared to healthy individuals. We deduced that emICH could rapidly trigger the dysbiosis of intestinal flora, and the disturbed microbiota could, in turn, exacerbate inflammatory response and increase the risk of hematoma expansion. Our comprehensive research revealed the potential of intestinal flora as a potent diagnostic tool, emphasizing its significance as a preventive target for HE.</p>","PeriodicalId":12458,"journal":{"name":"Frontiers in Cellular and Infection Microbiology","volume":"15 ","pages":"1462562"},"PeriodicalIF":4.6,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11830820/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143440141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modulation of virulence and metabolic profiles in <i>Klebsiella pneumoniae</i> under indole-mediated stress response.","authors":"Xueyao Fang, Yanhui Chen, Longhua Hu, Shumin Gu, Junqi Zhu, Yaping Hang, Xingwei Cao, Yanping Xiao, Hong Luo, Chuwen Zhao, Lianhua Xiao, Qiaoshi Zhong","doi":"10.3389/fcimb.2025.1546991","DOIUrl":"10.3389/fcimb.2025.1546991","url":null,"abstract":"<p><p>Indole, a crucial bacterial signaling molecule, plays a fundamental role in regulating various physiological processes within bacteria, including growth, acid tolerance, biofilm development, motility, and other cellular functions. Its regulatory influence extends beyond indole-producing bacteria, significantly impacting the physiological activities in non-indole-producing species. In this study, we demonstrate that indole enhances the pathogenicity and viability of <i>Klebsiella pneumoniae</i> using the <i>Galleria mellonella</i> infection model and serum killing assay. Concurrently, indole has varying effects on biofilm formation in <i>K. pneumoniae</i>, with some strains showing enhanced biofilm formation ability. To elucidate the underlying molecular mechanisms, transcriptome analysis revealed that indole exposure in <i>K. pneumoniae</i> led to the upregulation of genes associated with pili formation and iron acquisition systems, while simultaneously inducing oxidative stress responses. Additionally, our analysis uncovered extensive metabolic remodeling. Specifically, we observed significant upregulation of genes involved in simple carbohydrate utilization pathways, including those responsible for galactose, mannose, and maltose metabolism, as well as enhanced expression of genes associated with pyrimidine biosynthesis. These findings collectively indicate that indole enhances the intestinal colonization and pathogenicity of <i>K. pneumoniae</i> primarily by modulation of fimbriae expression and metabolic pathway regulation.</p>","PeriodicalId":12458,"journal":{"name":"Frontiers in Cellular and Infection Microbiology","volume":"15 ","pages":"1546991"},"PeriodicalIF":4.6,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11830697/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143440222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Editorial: The role of dietary interventions in the regulation of host-microbe interactions, volume II.","authors":"Xinyi Zhang, Yujie Cai, Xia He, Tingtao Chen","doi":"10.3389/fcimb.2025.1549914","DOIUrl":"https://doi.org/10.3389/fcimb.2025.1549914","url":null,"abstract":"","PeriodicalId":12458,"journal":{"name":"Frontiers in Cellular and Infection Microbiology","volume":"15 ","pages":"1549914"},"PeriodicalIF":4.6,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11830740/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143440139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of intracranial lesions in patients with HIV-associated cryptococcal meningitis.","authors":"Wei Song, Li Liu, Tangkai Qi, Zhenyan Wang, Yang Tang, Jianjun Sun, Shuibao Xu, Junyang Yang, Jiangrong Wang, Jun Chen, Renfang Zhang, Yinzhong Shen","doi":"10.3389/fcimb.2025.1446470","DOIUrl":"10.3389/fcimb.2025.1446470","url":null,"abstract":"<p><strong>Background: </strong>Intracranial imaging abnormalities are commonly observed in patients suffering from HIV-associated cryptococcal meningitis, both before and during the treatment period. This study aims to analyze the prevalence, origins, radiological characteristics, treatments, and prognosis of intracranial lesions in patients with HIV-associated cryptococcal meningitis, thereby providing references for future clinical decision-making.</p><p><strong>Methods: </strong>The clinical data of patients diagnosed with HIV-associated cryptococcal meningitis and admitted to the Shanghai Public Health Clinical Centre between 2013 and 2019 were collected. Logistic regression analysis was subsequently conducted to identify potential risk factors associated with the development of intracranial lesions in this patient group.</p><p><strong>Results: </strong>Of 211 patients analyzed, 64.5% (136/211) had intracranial lesions during treatment and follow-up. Initial cranial imaging showed 60% had lesions pre-treatment. Throughout treatment, 32.7% (52/159) developed new or worsened lesions. Mortality rates at 2 weeks, 8 weeks, and 2 years for those with detected lesions were 3%, 7.6%, and 13.2%, respectively. Lesions were primarily caused by <i>Cryptococcus</i> (70.5%) and <i>Mycobacterium</i> (24.3%). Lacunar infarcts, especially in the basal ganglia, were the most common type. Patients aged 50 years or older, and those presenting with altered mental status upon admission, were found to be more likely to have intracranial lesions at baseline, with adjusted odds ratios of 5.364 (95% CI: 1.468-19.591, P=0.011) and 7.970 (95% CI: 2.241-28.337, P=0.001), respectively. Patients with lesion progression showed higher levels of IFN-γ, IL-4, IL-5, IL-6, IL-1Ra, IL-1β, GM-CSF, Eotaxin, and Basic FGF in cerebrospinal fluid after four weeks of treatment.</p><p><strong>Conclusion: </strong>Intracranial lesions in HIV-associated cryptococcal meningitis patients are mostly due to Cryptococcus and Mycobacterium infections. They often appear as lacunar infarcts, predominantly in the basal ganglia, and can worsen with treatment initiation, possibly due to higher baseline cytokine levels in cerebrospinal fluid.</p>","PeriodicalId":12458,"journal":{"name":"Frontiers in Cellular and Infection Microbiology","volume":"15 ","pages":"1446470"},"PeriodicalIF":4.6,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11830730/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143439502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gut-brain axis mediated by intestinal content microbiota was associated with Zhishi Daozhi decoction on constipation.","authors":"Leyao Fang, Xin Yi, Junxi Shen, Na Deng, Xinxin Peng","doi":"10.3389/fcimb.2025.1539277","DOIUrl":"10.3389/fcimb.2025.1539277","url":null,"abstract":"<p><strong>Background: </strong>Constipation is a common digestive system disorder, which is closely related to the intestinal flora. Zhishi Daozhi decoction (ZDD) is a traditional Chinese medicine prescription used to treat constipation caused by indigestion. This study is to evaluate the efficacy of ZDD in treating constipation and to elucidate the underlying mechanism.</p><p><strong>Methods: </strong>In this study, Kunming mice were administered a high-protein diet (HFHPD) and loperamide hydrochloride injections to induce constipation. The mice then received varying doses (2.4, 4.7, and 9.4 mg/kg) of ZDD for seven days. Following the sampling process, we measured fecal microbial activity. The levels of 5-hydroxytryptamine (5-HT), vasoactive intestinal peptide (VIP), and aquaporin-3 (AQP3) were quantified using enzyme-linked immunosorbent assay. Changes in the gut microbiota were evaluated through 16S rRNA gene sequencing. Additionally, we investigated the correlation between specific microbiota features and the levels of 5-HT, VIP, and AQP3.</p><p><strong>Results: </strong>The fecal surface of the mice in the model group (CMM) was rough and dry. The stool of mice in the low-dose ZDD group (CLD), medium-dose ZDD group (CMD), and high-dose ZDD group (CHD) exhibited a smoother texture, closely resembling that of the normal group (CNM). 5-HT levels in the CMM group were significantly lower than in the CNM, CLD, and CHD. VIP levels in the CMD were lower than in the other four groups, and AQP3 levels in CMM showed a decreasing trend. The fecal microbial activity of the CMM group was significantly higher than that of the other groups. Diversity analysis indicated that CMD and CHD treatments were more effective in restoring the intestinal microbiota structure. Potential pathogenic bacteria, including <i>Clostridium</i>, <i>Aerococcus</i>, <i>Jeotgalicoccus</i>, and <i>Staphylococcus</i> were enriched in CMM. In contrast, beneficial bacteria such as <i>Faecalibacterium</i>, <i>Bacillaceae</i>, and <i>Bacillus</i> were more prevalent in the CLD, CMD, and CHD. Correlation analysis revealed that <i>Streptococcus</i> and <i>Enterococcus</i> were positively correlated with VIP, while <i>Succinivibrio</i> showed a negative correlation with 5-HT.</p><p><strong>Conclusions: </strong>Constipation induced by HFHPD and loperamide hydrochloride disrupts the structure of the intestinal microbiota. ZDD appears to alleviate constipation, potentially through mechanisms linked to the brain-gut axis and its interaction with the intestinal microbiota. Among the treatment groups, the medium dose of ZDD demonstrated the most effective results.</p>","PeriodicalId":12458,"journal":{"name":"Frontiers in Cellular and Infection Microbiology","volume":"15 ","pages":"1539277"},"PeriodicalIF":4.6,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11830728/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143440180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid and simple detection of <i>Candida albicans</i> using closed dumbbell-mediated isothermal amplification.","authors":"Yanli Zhang, Xuhan Chen, Yeling Zhong, Fei Guo, Guifang Ouyang, Rui Mao","doi":"10.3389/fcimb.2025.1484089","DOIUrl":"10.3389/fcimb.2025.1484089","url":null,"abstract":"<p><strong>Introduction: </strong><i>Candida albicans</i>, a human fungal pathogen, multiplies to invade body cells and causes fungal diseases in the condition of insufficient body's immune function. Early detection of <i>C. albicans</i> is required to guide appropriate prevention and treatment.</p><p><strong>Methods: </strong>The purpose of this study was to establish a <i>C. albicans</i> assay based on newly developed closed dumbbell-mediated isothermal amplification (CDA) to achieve rapid and simple point of care diagnostic. The CDA technique was carried out by specific primers targeting at the conserved <i>C. albicans</i> ITS2 gene. All primers were selected and evaluated by real-time fluorescence monitoring and endpoint visual judgement indicated by hydroxy naphthol blue (HNB). Optimal primers and accelerate primers (out primers and loop primers) were designed and selected after confirmation of the fundamental CDA primers to achieve more efficient CDA reaction for <i>C. albicans</i> detection (CA-OL-CDA).</p><p><strong>Results: </strong>After establishment of the assay, 9 <i>non-Candida albicans</i> strains, including 3 <i>Candida</i> species were tested to negative by adopting the established CA-OL-CDA assay, indicated high specificity. The limit of detection of <i>Candida albicans</i> DNA by CA-OL-CDA assay was 6.2×10^-6 ng/μL of DNA (10 copies/μL), 10-fold more sensitive than real-time quantitative PCR (qPCR).</p><p><strong>Discussion: </strong>The CA-OL-CDA assay exhibited advantages of high specificity, sensitivity, simpler and more efficient operation. In addition, the CA-OL-CDA method holds potential in on-site detection for <i>C. albicans</i> using color shift by adopting the reaction mixture based on HNB.</p>","PeriodicalId":12458,"journal":{"name":"Frontiers in Cellular and Infection Microbiology","volume":"15 ","pages":"1484089"},"PeriodicalIF":4.6,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11830661/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143440433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correlation between uterine microbiota and pregnancy outcomes of embryo transfer in overweight and obese women.","authors":"Ying Yan, Ying Li, Lingling Wu, Yuxuan Zhang, Hong Guo, Yazhong Ji","doi":"10.3389/fcimb.2025.1515563","DOIUrl":"10.3389/fcimb.2025.1515563","url":null,"abstract":"<p><strong>Objective: </strong>Currently, it has been reported that uterine microbiota affected pregnancy outcomes in assisted reproductive technology, but little was known in obese women. Thus, this study aims to explore how body weight affects pregnancy outcomes by comparing the differences in uterine microbiota between women of normal weight and those with obesity or overweight.</p><p><strong>Methods: </strong>The study included 45 embryo transfer cycles. Patients were divided into two groups based on body mass index (BMI): normal-weight group, BMI ≤23.9 kg/m² (Con group, n=31) and obesity/overweight group, BMI >23.9 kg/m² (OwOb group, n=14). Microbial samples were collected from the catheter tips and analyzed using RNA Ribosomal 16S.</p><p><strong>Results: </strong>In 45 women, the β-human chorionic gonadotropin (β-hCG) positivity rate and clinical pregnancy rate 10-12 days after embryo transfer were higher in the Con group. At the genus level, the relative abundance of Lactobacillus in the Con group was 2.2 times higher than that in the OwOb group. The Alpha diversity in the OwOb group was significantly higher than in the Con group (p=0.028). After regrouping based on β-hCG results 10-12 days post-transfer, in the Con-negative group (the group with negative β-hCG in the Con group, n=16) the relative abundances of pathogenic bacteria such as Klebsiella (p<0.001), Parasutterella (p=0.004), Dialister (p=0.01), and Gardnerella (p=0.029) were significantly higher than in the Con-positive group (the group with positive β-hCG in the Con group, n=15).</p><p><strong>Conclusion: </strong>Overweight and obese women possess a distinct uterine microbiota, characterized by a lower relative abundance of Lactobacillus and an increased relative abundance of pathogenic bacteria, along with specific genera strongly associated with obesity. In individuals with higher BMI, pathogenic bacteria are more likely to impair endometrial receptivity, ultimately leading to adverse pregnancy outcomes. Therefore, modulating the uterine microbiota in overweight/obese women may improve assisted reproductive technology success rates.</p>","PeriodicalId":12458,"journal":{"name":"Frontiers in Cellular and Infection Microbiology","volume":"15 ","pages":"1515563"},"PeriodicalIF":4.6,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11830673/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143440062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum: Editorial: Antiviral monoclonal antibody therapies.","authors":"Catalina Florez, Denise Haslwanter, Rohit K Jangra","doi":"10.3389/fcimb.2025.1558708","DOIUrl":"https://doi.org/10.3389/fcimb.2025.1558708","url":null,"abstract":"<p><p>[This corrects the article DOI: 10.3389/fcimb.2024.1484448.].</p>","PeriodicalId":12458,"journal":{"name":"Frontiers in Cellular and Infection Microbiology","volume":"15 ","pages":"1558708"},"PeriodicalIF":4.6,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11831045/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143440133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nineteen years retrospective analysis of epidemiology, antifungal resistance and a nomogram model for 30-day mortality in nosocomial candidemia patients.","authors":"Zhang Dai, Xuhong Lan, Minjing Cai, Yunhui Liao, Jingwen Zhang, Naifang Ye, Xinxin Lu, Jiajia Wang, Yun Xiao, Yan Zhang, Yihui Yao, Xianming Liang","doi":"10.3389/fcimb.2025.1504866","DOIUrl":"10.3389/fcimb.2025.1504866","url":null,"abstract":"<p><strong>Background: </strong>The incidence of nosocomial candidemia has increased in recently years, however, the epidemiological data remain insufficient in China.</p><p><strong>Methods: </strong>A total of 234 candidemia patients were included from Xiamen University Zhong Shan hospital between January 2006 and October 2024. Incidence, species proportion, distribution, antifungal drug resistance of candidemia was analyzed. A nomogram model for 30-day morbidity of candidemia was determined using the least absolute shrinkage and logistic regression analysis.</p><p><strong>Results: </strong>The incidence of candidemia increased in recent years (2020: 0.025%, 2021: 0.029%, 2023:0.022%). The dominant species of candidemia were <i>Candida albicans</i> (n=99,42.31%), <i>Candida parapsilosis</i> (n=47,20.09%), <i>Candida tropicalis</i> (n=43,18.38%), <i>Candida glabrata</i> (n=31,13.25%). Departments with a higher detection of candidemia included intensive care unit (n=55), emergency department (n=24) and hepatobiliary surgery (n=22). <i>Candida tropicalis</i> performed the highest resistance to azole (fluconazole: 55.81%, voriconazole:55.00% and itraconazole:58.14%). The resistance of <i>Candida albicans</i> to fluconazole, voriconazole and itraconazole were 32.32%, 23.53% and 31.31%. The mortality rate of 30-day discharge for candidemia reached 52.99%. 205 cases of candidemia patients from January 2006 to December 2023 were included as the training set, while 29 cases of candidiasis patients from January to October 2004 were included as the validation set. Five independent factors included <i>Candida albicans</i>, decreased albumin, multiple organ dysfunction syndrome, solid tumor and septic shock were adopted in a nomogram for 30-days mortality of candidemia. In the training set, the area under curve was 0.866 (95%CI: 0.817-0.916), the optimal cutoff value was 0.617, the sensitivity was 80% and the specificity was 80.4%. In the validation set, the area under curve was 0.808 (95%CI:0.737-0.970), the optimal cutoff value was 0.543. The sensitivity was 72.7% and the specificity was 83.3%.</p><p><strong>Conclusion: </strong>The incidence of nosocomial candidemia has risen in recent years. <i>Candida albicans</i> remains the primary species, with the highest incidence is intensive care unit. <i>Candida tropicalis</i> exhibits the highest resistance rate to azole drugs. A nomogram predicting 30-day mortality discharge for candidemia patients has been constructed, and the independent risk factors including <i>Candida albicans</i>, multiple organ dysfunction syndrome, septic shock, solid tumors, and decreased albumin.</p>","PeriodicalId":12458,"journal":{"name":"Frontiers in Cellular and Infection Microbiology","volume":"15 ","pages":"1504866"},"PeriodicalIF":4.6,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11830704/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143440430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}