Free Radical Research最新文献_第8页

Redox remodeling of central metabolism as a driving force for cellular protection, proliferation, differentiation, and dysfunction. 中枢代谢的氧化还原重塑是细胞保护、增殖、分化和功能障碍的驱动力。

IF 3.6 3区生物学

Free Radical Research Pub Date : 2024-10-01 Epub Date: 2024-09-24 DOI: 10.1080/10715762.2024.2407147

Junichi Fujii

{"title":"Redox remodeling of central metabolism as a driving force for cellular protection, proliferation, differentiation, and dysfunction.","authors":"Junichi Fujii","doi":"10.1080/10715762.2024.2407147","DOIUrl":"10.1080/10715762.2024.2407147","url":null,"abstract":"The production of reactive oxygen species (ROS) is elevated via metabolic hyperactivation in response to a variety of stimuli such as growth factors and inflammation. Tolerable amounts of ROS moderately inactivate enzymes via oxidative modification, which can be reversed back to the native form in a redox-dependent manner. The excessive production of ROS, however, causes cell dysfunction and death. Redox-reactive enzymes are present in primary metabolic pathways such as glycolysis and the tricarboxylic acid cycle, and these act as floodgates for carbon flux. Oxidation of a specific form of cysteine inhibits glyceraldehyde-3-phosphate dehydrogenase, which is reversible, and causes an accumulation of upstream intermediary compounds that increases the flux of glucose-6-phosphate to the pentose phosphate pathway. These reactions increase the NADPH and ribose-5-phosphate that are available for reductive reactions and nucleotide synthesis, respectively. On the other hand, oxidative inactivation of mitochondrial aconitase increases citrate, which is then recruited to synthesize fatty acids in the cytoplasm. Decreases in the use of carbohydrate for ATP production can be compensated via amino acid catabolism, and this metabolic change makes nitrogen available for nucleic acid synthesis. Coupling of the urea cycle also converts nitrogen to urea and polyamine, the latter of which supports cell growth. This metabolic remodeling stimulates the proliferation of tumor cells and fibrosis in oxidatively damaged tissues. Oxidative modification of these enzymes is generally reversible in the early stages of oxidizing reactions, which suggests that early treatment with appropriate antioxidants promotes the maintenance of natural metabolism.","PeriodicalId":12411,"journal":{"name":"Free Radical Research","volume":" ","pages":"606-629"},"PeriodicalIF":3.6,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142344616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

NRF2 signaling and amino acid metabolism in cancer. 癌症中的 NRF2 信号传导和氨基酸代谢。

IF 3.6 3区生物学

Free Radical Research Pub Date : 2024-10-01 Epub Date: 2024-11-14 DOI: 10.1080/10715762.2024.2423690

Suji Ham, Bo-Hyun Choi, Mi-Kyoung Kwak

引用次数: 0

Redox system and ROS-related disorders in peroxisomes. 过氧物酶体中的氧化还原系统和 ROS 相关紊乱。

IF 3.6 3区生物学

Free Radical Research Pub Date : 2024-10-01 Epub Date: 2024-11-17 DOI: 10.1080/10715762.2024.2427088

Hyunsoo Kim, Jaetaek Hwang, Channy Park, Raekil Park

引用次数: 0

The inhibitory potential of 4,7-dihydroxycoumarin derivatives on ROS-producing enzymes and direct HOO•/o2• - radical scavenging activity - a comprehensive kinetic DFT study. 4,7-二羟基香豆素衍生物对产生 ROS 的酶的抑制潜力和直接清除 HOO-/o2- 自由基的活性--一项全面的动力学 DFT 研究。

IF 3.3 3区生物学

Free Radical Research Pub Date : 2024-09-12 DOI: 10.1080/10715762.2024.2400674

Žiko Milanović,Svetlana Jeremić,Marko Antonijević,Dušan Dimić,Đura Nakarada,Edina Avdović,Zoran Marković

{"title":"The inhibitory potential of 4,7-dihydroxycoumarin derivatives on ROS-producing enzymes and direct HOO•/o2• - radical scavenging activity - a comprehensive kinetic DFT study.","authors":"Žiko Milanović,Svetlana Jeremić,Marko Antonijević,Dušan Dimić,Đura Nakarada,Edina Avdović,Zoran Marković","doi":"10.1080/10715762.2024.2400674","DOIUrl":"https://doi.org/10.1080/10715762.2024.2400674","url":null,"abstract":"This study examined the antiradical activity of three synthesized coumarin derivatives: (E)-3-(1-((2-hydroxyphenyl)amino)ethylidene)-2,4-dioxochroman-7-yl acetate (A1-OH), (E)-3-(1-((3-hydroxyphenyl)amino)ethylidene)-2,4-dioxochroman-7-yl acetate (A2-OH), and (E)-3-(1-((4-hydroxyphenyl)amino)ethylidene)-2,4-dioxochroman-7-yl acetate (A3-OH) against HOO•/O2•- radical species. The investigation included electron spin resonance (ESR) measurements and a DFT kinetic study. Thermodynamic and kinetic parameters of antiradical mechanisms-Formal Hydrogen Atom Transfer (f-HAT), Radical Adduct Formation (RAF), Sequential Proton Loss followed by Electron Transfer (SPLET), and Single-Electron Transfer followed by Proton Transfer (SET-PT)-were evaluated using the Quantum Mechanics-based test for Overall Free Radical Scavenging Activity (QM-ORSA) under physiological conditions. ESR results indicated antiradical activity decreased in the sequence A1-OH (58.7%) > A2-OH (57.5%) > A3-OH (53.1%). Kinetic analysis revealed the f-HAT mechanism dominated HOO• inactivation. A newly formulated Sequential Proton Loss followed by Radical Adduct Formation (SPL-RAF) mechanism described interactions with O2•-. The activity toward O2•- was A2-OH (1.26 × 106 M-1s-1) > A3-OH (7.71 × 105 M-1s-1) > A1-OH (4.22 × 105 M-1s-1). Molecular docking and dynamics studies tested inhibitory capability against enzymes producing reactive species: Lipoxygenase (LOX), Myeloperoxidase (MPO), NAD(P)H oxidase (NOX), and Xanthine Oxidase (XOD). Affinity to enzymes decreased in the order: XOD > LOX > NOX > MPO.","PeriodicalId":12411,"journal":{"name":"Free Radical Research","volume":"27 1","pages":"1-16"},"PeriodicalIF":3.3,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142219710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

EP4 receptor agonist CAY10598 upregulates ROS-dependent Hsp90 cleavage in colorectal cancer cells. EP4受体激动剂CAY10598能上调结直肠癌细胞中依赖于ROS的Hsp90裂解。

IF 3.3 3区生物学

Free Radical Research Pub Date : 2024-09-11 DOI: 10.1080/10715762.2024.2396909

In Gyung Chae,Joohee Jung,Do-Hee Kim,Joon-Seok Choi,Kyung-Soo Chun

{"title":"EP4 receptor agonist CAY10598 upregulates ROS-dependent Hsp90 cleavage in colorectal cancer cells.","authors":"In Gyung Chae,Joohee Jung,Do-Hee Kim,Joon-Seok Choi,Kyung-Soo Chun","doi":"10.1080/10715762.2024.2396909","DOIUrl":"https://doi.org/10.1080/10715762.2024.2396909","url":null,"abstract":"Prostaglandin E2 (PGE2) interacts with four specific G protein-coupled receptors, namely EP1, EP2, EP3, and EP4, playing a pivotal role in determining the fate of cells. Our previous findings highlighted that stimulating the EP4 receptor with its agonist, CAY10598, triggers apoptosis in colon cancer HCT116 cells via the production of reactive oxygen species (ROS). This process also reduces the phosphorylation of the oncogenic protein JAK2 and leads to its degradation in these cells. In this study, our goal was to explore the pathways through which CAY10598 leads to JAK2 degradation. We focused on Hsp90, a heat shock protein family member known for its role as a molecular chaperone maintaining the stability of several key proteins including EGFR, MET, Akt, and JAK2. Our results show that CAY10598 decreases the levels of client proteins of Hsp90 in HCT116 cells, an effect reversible by pretreatment with the ROS scavenger N-acetyl cysteine (NAC) or the proteasome inhibitor MG132, indicating that the degradation is likely driven by ROS. Furthermore, we observed that CAY10598 cleaves both α and β isoforms of Hsp90, the process inhibited by NAC. Inhibition of EP4 with the antagonist GW627368x not only prevented the degradation of Hsp90 client proteins but also the cleavage of Hsp90 itself in CAY10598-treated HCT116 cells. Additionally, CAY10598 suppressed the growth of HCT116 cells implanted in mice. Our findings reveal that CAY10598 induces apoptosis in cancer cells by a novel mechanism involving the ROS-dependent cleavage of Hsp90, thereby inhibiting the function of crucial Hsp90 client proteins.","PeriodicalId":12411,"journal":{"name":"Free Radical Research","volume":"6 1","pages":"1-10"},"PeriodicalIF":3.3,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142219711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Low density lipoprotein oxidized under lysosomal conditions decreases arterial vasodilatation. 在溶酶体条件下氧化的低密度脂蛋白会降低动脉血管舒张功能。

IF 3.6 3区生物学

Free Radical Research Pub Date : 2024-08-01 Epub Date: 2024-09-13 DOI: 10.1080/10715762.2024.2403038

Hadeel K M Alboaklah, Alister J McNeish, David S Leake

{"title":"Low density lipoprotein oxidized under lysosomal conditions decreases arterial vasodilatation.","authors":"Hadeel K M Alboaklah, Alister J McNeish, David S Leake","doi":"10.1080/10715762.2024.2403038","DOIUrl":"10.1080/10715762.2024.2403038","url":null,"abstract":"Endothelial dysfunction is a risk factor for atherosclerosis and includes impaired endothelium-dependent vasodilatation. We have shown previously that low density lipoprotein (LDL) can be oxidized by iron in the lysosomes of macrophages. Macrophage lysis in atherosclerotic lesions might expose endothelial cells to this oxidized LDL and adversely affect their function. LDL was oxidized by ferrous sulfate (5 µM) for 24 h at pH 4.5 at 37 °C. Aortas from male Wistar rats were cut into rings and subjected to wire myography for isometric tension recording. The rings were incubated with or without oxidized LDL (50 µg protein/ml) for one hour, constricted with 100 nM phenylephrine and relaxation to acetylcholine (1 nM - 3 µM) was measured. There was about 50% less relaxation in the presence of this oxidized LDL. Endothelial-independent vasodilatation induced by sodium nitroprusside was less affected by oxidized LDL. Oxidized LDL increased the formation of reactive oxygen species by the aortic rings and by cultured human aortic and dermal microvascular endothelial cells, which might have inactivated nitric oxide. Acetylcholine increased the activatory phosphorylation of eNOS (ser-1177), but oxidized LDL had little effect on this activation in cultured human aortic endothelial cells. These findings raise the possibility that LDL oxidized in lysosomes and released from lysed macrophages might decrease vasodilatation in atherosclerotic arteries.","PeriodicalId":12411,"journal":{"name":"Free Radical Research","volume":" ","pages":"509-516"},"PeriodicalIF":3.6,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142282999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effect of hypochlorous acid inhalation on the activity of antioxidant system enzymes in rats of different ages. 吸入次氯酸对不同年龄大鼠抗氧化系统酶活性的影响。

IF 3.6 3区生物学

Free Radical Research Pub Date : 2024-08-01 DOI: 10.1080/10715762.2024.2386688

Bohdan Murashevych, Hanna Maslak, Dmitry Girenko, Olha Abraimova, Olha Netronina, Volodymyr Shvets

{"title":"The effect of hypochlorous acid inhalation on the activity of antioxidant system enzymes in rats of different ages.","authors":"Bohdan Murashevych, Hanna Maslak, Dmitry Girenko, Olha Abraimova, Olha Netronina, Volodymyr Shvets","doi":"10.1080/10715762.2024.2386688","DOIUrl":"10.1080/10715762.2024.2386688","url":null,"abstract":"Hypochlorous acid HOCl is an effective disinfectant with a broad spectrum and high rate of microbicidal action. Its use for air treatment can be an effective tool for the prevention and therapy of infectious diseases. In this work, the in vivo study was conducted on 110 Wistar Han rats (12 and 72 weeks old) on the effect of a single inhalation of air containing gaseous HOCl on the activity of antioxidant system enzymes. For this, a special installation was designed to uniformly maintain the concentration of HOCl in the air and regulate it over a wide range. Inhalation exposure was carried out for 4 h at total chlorine concentrations in the air of approximately 2.0 mg/m3 and 5.0 mg/m3, after which the animals were observed for 14 days. The effect of inhalation on the antioxidant system activity varied significantly in animals of different ages. Catalase activity in young rats increased approximately 2-fold on days 1-2 after inhalation, regardless of the HOCl concentration, while in old animals a sharp dose-dependent decrease was initially observed. The glutathione peroxidase activity in animals of both ages increased upon inhalation of air with 5.0 mg/m3 HOCl, and in old animals this was more pronounced; when the HOCl concentration decreased to 2.0 mg/m3, this indicator increased slightly in old rats and remained virtually unchanged in young ones. The glutathione reductase activity when exposed to 2.0 mg/m3 HOCl did not change for both age groups, and with increasing HOCl concentration it increased by 1.5-2.0 times in all animals.","PeriodicalId":12411,"journal":{"name":"Free Radical Research","volume":" ","pages":"441-457"},"PeriodicalIF":3.6,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141792363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Polyphenolic metacyclophane as a radical scavenger for therapeutic activation: a computational study. 多酚类化合物 Metacyclophane 作为自由基清除剂用于激活疗法：计算研究。

IF 3.6 3区生物学

Free Radical Research Pub Date : 2024-08-01 Epub Date: 2024-08-22 DOI: 10.1080/10715762.2024.2394121

Raktim Nath, Alaiha Zaheen, Sanchaita Rajkhowa, Rahul Kar

{"title":"Polyphenolic metacyclophane as a radical scavenger for therapeutic activation: a computational study.","authors":"Raktim Nath, Alaiha Zaheen, Sanchaita Rajkhowa, Rahul Kar","doi":"10.1080/10715762.2024.2394121","DOIUrl":"10.1080/10715762.2024.2394121","url":null,"abstract":"Modeling antioxidants for improved human health is a prime area of research. Inclusion complexes exhibit antioxidant activity. Supramolecular scaffolds like calixtyrosol are anticipated to have considerable antioxidant and therapeutic activity. In this study, we have designed 30 polyphenolic metacyclophanes and investigated their antioxidant properties. Exceptional O─H bond dissociation energy of 44 kcal/mol is reported for a metacyclophane with acyl urea linkage. This may be explained through a cooperative effect of localization of spin density distribution and an intramolecular hydrogen bonding of the corresponding radical. Further, the pharmacokinetics and toxicity analysis screened eight drug-like candidates. The interaction of the eight screened molecules with the Lysozyme transport protein and SOD protein has been studied using the molecular docking approach. Lastly, the MD simulations are performed to analyze the conformational changes of the transport protein after complexation with the proposed molecules. Comprehensive analyses including density functional studies of physiological parameters, favorable pharmacokinetics, toxicity, molecular docking, and MD simulations affirmed polyphenolic metacyclophane XXI as a radical scavenging and drug-like candidate.","PeriodicalId":12411,"journal":{"name":"Free Radical Research","volume":" ","pages":"476-492"},"PeriodicalIF":3.6,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141999713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reduction of oxidative damage in prostate tissue caused by radiation and/or chloroquine by apocynin. 阿朴昔宁可减少辐射和/或氯喹对前列腺组织造成的氧化损伤。

IF 3.6 3区生物学

Free Radical Research Pub Date : 2024-08-01 Epub Date: 2024-08-20 DOI: 10.1080/10715762.2024.2393147

Onur Ertik, Ayca Sezen Us, Ilknur Bugan Gul, Huseyin Us, Melis Coremen, Omur Karabulut Bulan, Refiye Yanardag

{"title":"Reduction of oxidative damage in prostate tissue caused by radiation and/or chloroquine by apocynin.","authors":"Onur Ertik, Ayca Sezen Us, Ilknur Bugan Gul, Huseyin Us, Melis Coremen, Omur Karabulut Bulan, Refiye Yanardag","doi":"10.1080/10715762.2024.2393147","DOIUrl":"10.1080/10715762.2024.2393147","url":null,"abstract":"Prostate damage can occur in men due to age and genetic factors, especially when exposed to external factors. Radiation (RAD) is a prominent factor leading to oxidative stress and potential prostate damage. Additionally, chloroquine (CQ), used in malaria treatment, can induce oxidative stress in a dose-dependent manner. Therefore, reducing and preventing oxidative damage in prostate tissue caused by external factors is crucial. Rats used in the study were divided into seven groups, CQ, apocynin (APO), RAD, CQ + APO, CQ + RAD, APO + RAD, CQ + APO + RAD. Subsequently, in vivo biochemical parameters of prostate tissues were examined, including reduced glutathione, lipid peroxidation, superoxide dismutase, glutathione reductase, glutathione peroxidase, glutathione-S-transferase activities, and total antioxidant status, total oxidant status, reactive oxygen species, oxidative stress index, advanced oxidation protein products and histologically. The in vivo results presented in our study showed that APO reduced oxidative stress and had a protective effect on prostate tissue in the CQ, RAD, and CQ + RAD groups as a results of biochemical and histological experiments. Additionally, in silico studies revealed a higher binding affinity of diapocynin to target proteins compared to APO. As a histological results, RAD and CQ alone or in combination did not induce damage in prostate tissues, whereas mild histopathological findings such as hyperemia and haemorrhage were observed in all APO-treated groups. The results suggest that the use of APO for the treatment of oxidative damage induced by CQ and RAD in rats.","PeriodicalId":12411,"journal":{"name":"Free Radical Research","volume":" ","pages":"458-475"},"PeriodicalIF":3.6,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141987796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction. 更正。

IF 3.6 3区生物学

Free Radical Research Pub Date : 2024-05-01 Epub Date: 2024-07-25 DOI: 10.1080/10715762.2024.2381328

引用次数: 0