Free Radical Research最新文献_第4页

Low density lipoprotein oxidized under lysosomal conditions decreases arterial vasodilatation. 在溶酶体条件下氧化的低密度脂蛋白会降低动脉血管舒张功能。

IF 3.6 3区生物学

Free Radical Research Pub Date : 2024-08-01 Epub Date: 2024-09-13 DOI: 10.1080/10715762.2024.2403038

Hadeel K M Alboaklah, Alister J McNeish, David S Leake

{"title":"Low density lipoprotein oxidized under lysosomal conditions decreases arterial vasodilatation.","authors":"Hadeel K M Alboaklah, Alister J McNeish, David S Leake","doi":"10.1080/10715762.2024.2403038","DOIUrl":"10.1080/10715762.2024.2403038","url":null,"abstract":"Endothelial dysfunction is a risk factor for atherosclerosis and includes impaired endothelium-dependent vasodilatation. We have shown previously that low density lipoprotein (LDL) can be oxidized by iron in the lysosomes of macrophages. Macrophage lysis in atherosclerotic lesions might expose endothelial cells to this oxidized LDL and adversely affect their function. LDL was oxidized by ferrous sulfate (5 µM) for 24 h at pH 4.5 at 37 °C. Aortas from male Wistar rats were cut into rings and subjected to wire myography for isometric tension recording. The rings were incubated with or without oxidized LDL (50 µg protein/ml) for one hour, constricted with 100 nM phenylephrine and relaxation to acetylcholine (1 nM - 3 µM) was measured. There was about 50% less relaxation in the presence of this oxidized LDL. Endothelial-independent vasodilatation induced by sodium nitroprusside was less affected by oxidized LDL. Oxidized LDL increased the formation of reactive oxygen species by the aortic rings and by cultured human aortic and dermal microvascular endothelial cells, which might have inactivated nitric oxide. Acetylcholine increased the activatory phosphorylation of eNOS (ser-1177), but oxidized LDL had little effect on this activation in cultured human aortic endothelial cells. These findings raise the possibility that LDL oxidized in lysosomes and released from lysed macrophages might decrease vasodilatation in atherosclerotic arteries.","PeriodicalId":12411,"journal":{"name":"Free Radical Research","volume":" ","pages":"509-516"},"PeriodicalIF":3.6,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142282999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effect of hypochlorous acid inhalation on the activity of antioxidant system enzymes in rats of different ages. 吸入次氯酸对不同年龄大鼠抗氧化系统酶活性的影响。

IF 3.6 3区生物学

Free Radical Research Pub Date : 2024-08-01 DOI: 10.1080/10715762.2024.2386688

Bohdan Murashevych, Hanna Maslak, Dmitry Girenko, Olha Abraimova, Olha Netronina, Volodymyr Shvets

{"title":"The effect of hypochlorous acid inhalation on the activity of antioxidant system enzymes in rats of different ages.","authors":"Bohdan Murashevych, Hanna Maslak, Dmitry Girenko, Olha Abraimova, Olha Netronina, Volodymyr Shvets","doi":"10.1080/10715762.2024.2386688","DOIUrl":"10.1080/10715762.2024.2386688","url":null,"abstract":"Hypochlorous acid HOCl is an effective disinfectant with a broad spectrum and high rate of microbicidal action. Its use for air treatment can be an effective tool for the prevention and therapy of infectious diseases. In this work, the in vivo study was conducted on 110 Wistar Han rats (12 and 72 weeks old) on the effect of a single inhalation of air containing gaseous HOCl on the activity of antioxidant system enzymes. For this, a special installation was designed to uniformly maintain the concentration of HOCl in the air and regulate it over a wide range. Inhalation exposure was carried out for 4 h at total chlorine concentrations in the air of approximately 2.0 mg/m3 and 5.0 mg/m3, after which the animals were observed for 14 days. The effect of inhalation on the antioxidant system activity varied significantly in animals of different ages. Catalase activity in young rats increased approximately 2-fold on days 1-2 after inhalation, regardless of the HOCl concentration, while in old animals a sharp dose-dependent decrease was initially observed. The glutathione peroxidase activity in animals of both ages increased upon inhalation of air with 5.0 mg/m3 HOCl, and in old animals this was more pronounced; when the HOCl concentration decreased to 2.0 mg/m3, this indicator increased slightly in old rats and remained virtually unchanged in young ones. The glutathione reductase activity when exposed to 2.0 mg/m3 HOCl did not change for both age groups, and with increasing HOCl concentration it increased by 1.5-2.0 times in all animals.","PeriodicalId":12411,"journal":{"name":"Free Radical Research","volume":" ","pages":"441-457"},"PeriodicalIF":3.6,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141792363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Polyphenolic metacyclophane as a radical scavenger for therapeutic activation: a computational study. 多酚类化合物 Metacyclophane 作为自由基清除剂用于激活疗法：计算研究。

IF 3.6 3区生物学

Free Radical Research Pub Date : 2024-08-01 Epub Date: 2024-08-22 DOI: 10.1080/10715762.2024.2394121

Raktim Nath, Alaiha Zaheen, Sanchaita Rajkhowa, Rahul Kar

{"title":"Polyphenolic metacyclophane as a radical scavenger for therapeutic activation: a computational study.","authors":"Raktim Nath, Alaiha Zaheen, Sanchaita Rajkhowa, Rahul Kar","doi":"10.1080/10715762.2024.2394121","DOIUrl":"10.1080/10715762.2024.2394121","url":null,"abstract":"Modeling antioxidants for improved human health is a prime area of research. Inclusion complexes exhibit antioxidant activity. Supramolecular scaffolds like calixtyrosol are anticipated to have considerable antioxidant and therapeutic activity. In this study, we have designed 30 polyphenolic metacyclophanes and investigated their antioxidant properties. Exceptional O─H bond dissociation energy of 44 kcal/mol is reported for a metacyclophane with acyl urea linkage. This may be explained through a cooperative effect of localization of spin density distribution and an intramolecular hydrogen bonding of the corresponding radical. Further, the pharmacokinetics and toxicity analysis screened eight drug-like candidates. The interaction of the eight screened molecules with the Lysozyme transport protein and SOD protein has been studied using the molecular docking approach. Lastly, the MD simulations are performed to analyze the conformational changes of the transport protein after complexation with the proposed molecules. Comprehensive analyses including density functional studies of physiological parameters, favorable pharmacokinetics, toxicity, molecular docking, and MD simulations affirmed polyphenolic metacyclophane XXI as a radical scavenging and drug-like candidate.","PeriodicalId":12411,"journal":{"name":"Free Radical Research","volume":" ","pages":"476-492"},"PeriodicalIF":3.6,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141999713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reduction of oxidative damage in prostate tissue caused by radiation and/or chloroquine by apocynin. 阿朴昔宁可减少辐射和/或氯喹对前列腺组织造成的氧化损伤。

IF 3.6 3区生物学

Free Radical Research Pub Date : 2024-08-01 Epub Date: 2024-08-20 DOI: 10.1080/10715762.2024.2393147

Onur Ertik, Ayca Sezen Us, Ilknur Bugan Gul, Huseyin Us, Melis Coremen, Omur Karabulut Bulan, Refiye Yanardag

{"title":"Reduction of oxidative damage in prostate tissue caused by radiation and/or chloroquine by apocynin.","authors":"Onur Ertik, Ayca Sezen Us, Ilknur Bugan Gul, Huseyin Us, Melis Coremen, Omur Karabulut Bulan, Refiye Yanardag","doi":"10.1080/10715762.2024.2393147","DOIUrl":"10.1080/10715762.2024.2393147","url":null,"abstract":"Prostate damage can occur in men due to age and genetic factors, especially when exposed to external factors. Radiation (RAD) is a prominent factor leading to oxidative stress and potential prostate damage. Additionally, chloroquine (CQ), used in malaria treatment, can induce oxidative stress in a dose-dependent manner. Therefore, reducing and preventing oxidative damage in prostate tissue caused by external factors is crucial. Rats used in the study were divided into seven groups, CQ, apocynin (APO), RAD, CQ + APO, CQ + RAD, APO + RAD, CQ + APO + RAD. Subsequently, in vivo biochemical parameters of prostate tissues were examined, including reduced glutathione, lipid peroxidation, superoxide dismutase, glutathione reductase, glutathione peroxidase, glutathione-S-transferase activities, and total antioxidant status, total oxidant status, reactive oxygen species, oxidative stress index, advanced oxidation protein products and histologically. The in vivo results presented in our study showed that APO reduced oxidative stress and had a protective effect on prostate tissue in the CQ, RAD, and CQ + RAD groups as a results of biochemical and histological experiments. Additionally, in silico studies revealed a higher binding affinity of diapocynin to target proteins compared to APO. As a histological results, RAD and CQ alone or in combination did not induce damage in prostate tissues, whereas mild histopathological findings such as hyperemia and haemorrhage were observed in all APO-treated groups. The results suggest that the use of APO for the treatment of oxidative damage induced by CQ and RAD in rats.","PeriodicalId":12411,"journal":{"name":"Free Radical Research","volume":" ","pages":"458-475"},"PeriodicalIF":3.6,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141987796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction. 更正。

IF 3.6 3区生物学

Free Radical Research Pub Date : 2024-05-01 Epub Date: 2024-07-25 DOI: 10.1080/10715762.2024.2381328

引用次数: 0

Hydroxyl radical scavenging and chemical repair capabilities of positively charged peptides (PCPs): a pulse radiolysis study. 正电荷肽（PCPs）的羟自由基清除和化学修复能力：脉冲辐射分解研究。

IF 3.6 3区生物学

Free Radical Research Pub Date : 2024-05-01 Epub Date: 2024-08-08 DOI: 10.1080/10715762.2024.2385342

Chaozhong Tian, Shinichi Yamashita, Atsushi Kimura, Yui Obata, Hao Yu, Mitsumasa Taguchi

{"title":"Hydroxyl radical scavenging and chemical repair capabilities of positively charged peptides (PCPs): a pulse radiolysis study.","authors":"Chaozhong Tian, Shinichi Yamashita, Atsushi Kimura, Yui Obata, Hao Yu, Mitsumasa Taguchi","doi":"10.1080/10715762.2024.2385342","DOIUrl":"10.1080/10715762.2024.2385342","url":null,"abstract":"Pulse radiolysis was employed to investigate fundamental radiation chemical reactions, which are essential in the radiation protection of DNA. Two positively charged peptides (PCPs), histidine-tyrosine-histidine (His-Tyr-His) and lysine-tyrosine-lysine (Lys-Tyr-Lys), as well as the amino acids that constitute them, were involved. The reaction rate constants for tyrosine (Tyr), histidine (His), lysine (Lys), His-Tyr-His, and Lys-Tyr-Lys with OH radicals (•OH) were (1.6 ± 0.3) × 1010, (9.0 ± 0.9) × 109, (1.4 ± 0.3) × 109, (1.8 ± 0.1) × 1010, and (1.0 ± 0.1) × 1010 M-1s-1, respectively, indicating that formation of peptide bond can affect the reaction of amino acids with •OH. Observed transient absorption spectra indicated a shielding effect of the His or Lys residues at both ends of the PCPs on the centrally located Tyr. The measurement of chemical repair capabilities using deoxyguanosine monophosphate (dGMP) as a model for DNA demonstrated that the reaction rate constants of Tyr, His-Tyr-His, and Lys-Tyr-Lys with dGMP radicals were (2.2 ± 0.5) × 108, (2.3 ± 0.1) × 108, and (3.3 ± 0.4) × 108 M-1s-1, respectively, implying that the presence of a positive charge may enhance the chemical repair process.","PeriodicalId":12411,"journal":{"name":"Free Radical Research","volume":" ","pages":"388-395"},"PeriodicalIF":3.6,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141901489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unveiling an oxidative stress-linked diagnostic signature and molecular subtypes in preeclampsia: novel insights into pathogenesis. 揭示与氧化应激相关的子痫前期诊断特征和分子亚型：对发病机制的新见解。

IF 3.6 3区生物学

Free Radical Research Pub Date : 2024-05-01 Epub Date: 2024-06-04 DOI: 10.1080/10715762.2024.2360015

Rurong Mao, Li Li, Penghao Li

{"title":"Unveiling an oxidative stress-linked diagnostic signature and molecular subtypes in preeclampsia: novel insights into pathogenesis.","authors":"Rurong Mao, Li Li, Penghao Li","doi":"10.1080/10715762.2024.2360015","DOIUrl":"10.1080/10715762.2024.2360015","url":null,"abstract":"Preeclampsia (PE) is a complex pregnancy disorder characterized by hypertension and organ dysfunction, affecting both maternal and fetal health. Oxidative stress has been implicated in the pathogenesis of PE, but the underlying molecular mechanisms remain poorly understood. In this study, we aimed to identify a diagnostic signature and molecular subtypes associated with oxidative stress in PE to gain novel insights into its pathogenesis. The ssGSEA algorithm evaluated oxidative stress-related pathway scores using transcriptional data from the GSE75010 dataset. Oxidative stress-related genes (ORGs) were co lected from these pathways, and hub ORGs associated with PE were identified using the LASSO and logistic regression models. A nomogram prediction model was constructed using the identified ORGs. Consensus clustering identified two molecular subgroups related to oxidative stress, labeled as C1 and C2, with unique immune characteristics and inflammatory pathway profiles. Seventy ORGs associated with oxidative stress, ce l death, and inflammation-related pathways were identified in PE. EGFR, RIPK3, and ALAD were confirmed as core ORGs for PE biomarkers. The C1 and C2 subgroups exhibited distinct immune characteristics and inflammatory pathway profiles. This study provides novel insights into the role of oxidative stress in PE pathogenesis. A diagnostic signature and molecular subtypes associated with oxidative stress were identified, which may improve understanding, diagnosis, and management of PE.","PeriodicalId":12411,"journal":{"name":"Free Radical Research","volume":" ","pages":"354-365"},"PeriodicalIF":3.6,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141093378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ferroptosis: a new target for hepatic ischemia-reperfusion injury? 铁蛋白沉积：肝缺血再灌注损伤的新靶点？

IF 3.6 3区生物学

Free Radical Research Pub Date : 2024-05-01 Epub Date: 2024-07-31 DOI: 10.1080/10715762.2024.2386075

Shanshan Guo, Zexin Li, Yi Liu, Ying Cheng, Degong Jia

引用次数: 0

Regulation of cancer cell ferroptosis by PTRF/Cavin-1. PTRF/Cavin-1 对癌细胞铁突变的调控。

IF 3.6 3区生物学

Free Radical Research Pub Date : 2024-05-01 Epub Date: 2024-08-07 DOI: 10.1080/10715762.2024.2386457

Hui Xiang, Miao Wang, Yi-Fang Chen, Hao-Ming Wu, Ming-Ge Li, Lei Guo, Ying-Yi Zhang, He-Zhe Lu

{"title":"Regulation of cancer cell ferroptosis by PTRF/Cavin-1.","authors":"Hui Xiang, Miao Wang, Yi-Fang Chen, Hao-Ming Wu, Ming-Ge Li, Lei Guo, Ying-Yi Zhang, He-Zhe Lu","doi":"10.1080/10715762.2024.2386457","DOIUrl":"10.1080/10715762.2024.2386457","url":null,"abstract":"Ovarian cancer, marked by high rate of recurrence, novel therapeutic strategies are needed to improve patient outcome. One of the potential strategies is inducing ferroptosis in ovarian cancer cells. Ferroptosis is an iron-dependent, lipid peroxidation-driven mode of cell death primarily occurring on the cell membrane. PTRF, an integral component of the caveolae structures located on the cell membrane, is involved in a multitude of physiological processes, including but not limited to, endocytosis, signal transduction, and lipid metabolism. This study elucidates the relationship between PTRF and ferroptosis in ovarian cancer, offering a fresh perspective for the development of new therapeutic strategies. We knocked down PTRF employing siRNA in the ovarian cancer cell lines HEY and SKOV3, following which we stimulated ferroptosis with Erastin (Era). Our research indicates that the lack of PTRF sensitizes cancer cells to ferroptosis, likely by altering membrane stability and tension, thereby affecting signal pathways related to ferroptosis, such as lipid and atherosclerosis, fluid shear stress, and atherosclerosis. Our findings provide new insights for developing new treatments for ovarian cancer.","PeriodicalId":12411,"journal":{"name":"Free Radical Research","volume":" ","pages":"417-429"},"PeriodicalIF":3.6,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141855324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antioxidant properties of α-amino acids: a density functional theory viewpoint. α-氨基酸的抗氧化特性：密度泛函理论观点。

IF 3.6 3区生物学

Free Radical Research Pub Date : 2024-05-01 Epub Date: 2024-08-05 DOI: 10.1080/10715762.2024.2385338

Andrey A Buglak

引用次数: 0