Free Radical Research最新文献_第4页

Accumulation of polyunsaturated lipids fuels ferroptosis to promote liver failure after extended hepatectomy in mice. 多不饱和脂质的积累助长了铁变态反应，从而导致小鼠肝切除术后肝功能衰竭。

IF 3.6 3区生物学

Free Radical Research Pub Date : 2024-11-01 Epub Date: 2024-11-08 DOI: 10.1080/10715762.2024.2423691

Can Huang, Jian Gan, Xiangyue Mo, Qingping Li, Leyi Liao, Biao Wang, Xianqiu Wu, Hanbiao Liang, Chen Xie, Tianzhou Peng, Yang Lei, Baoxiong Zhuang, Minghui Zeng, Yonghong Peng, Yisi Chen, Cuiting Liu, Jie Zhou, Kai Wang, Chuanjiang Li

{"title":"Accumulation of polyunsaturated lipids fuels ferroptosis to promote liver failure after extended hepatectomy in mice.","authors":"Can Huang, Jian Gan, Xiangyue Mo, Qingping Li, Leyi Liao, Biao Wang, Xianqiu Wu, Hanbiao Liang, Chen Xie, Tianzhou Peng, Yang Lei, Baoxiong Zhuang, Minghui Zeng, Yonghong Peng, Yisi Chen, Cuiting Liu, Jie Zhou, Kai Wang, Chuanjiang Li","doi":"10.1080/10715762.2024.2423691","DOIUrl":"10.1080/10715762.2024.2423691","url":null,"abstract":"Background: Post-hepatectomy liver failure (PHLF) is a fatal complication of hepatectomy. However, the mechanism of hepatocyte injury in PHLF remains elusive.Methods: PHLF was induced by extended 86% hepatectomy (eHx) in mice. Lipidomics was performed to investigate the eHx-induced lipid alteration in the residual liver. Ferroptosis was assessed to screen the hepatocyte injury induced by eHx. The therapeutic effects of ferrostatin-1 (Fer-1) on PHLF were evaluated.Results: PHLF was induced by eHx with elevation in markers of hepatocyte injury and mortality in mice within 48 h after surgery. eHx-induced hepatocyte injury was manifested by hepatocyte enlargement and hepatocyte death with glycogen depletion and lipid accumulation. Lipidomics revealed that eHx induced the accumulation of ferroptosis-favored polyunsaturated lipids. Ferroptosis was found to mediate the eHx-induced hepatocyte death in the residual liver during the development of PHLF. Fer-1 could attenuate the eHx-induced ferroptotic hepatocyte death and PHLF in mice.Conclusions: Ferroptosis partly mediates the eHx-induced hepatocyte injury during the development of PHLF. Accumulation of polyunsaturated lipids in hepatocytes may promote eHx-induced ferroptosis, and targeting lipid peroxidation is a potential therapeutic strategy for PHLF.","PeriodicalId":12411,"journal":{"name":"Free Radical Research","volume":" ","pages":"733-747"},"PeriodicalIF":3.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142603925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Modifications of DJ-1 in which pI shifts to acidic in red blood cells a potential biomarker for Parkinson's disease at early stages. 红细胞中 pI 变为酸性的 DJ-1 修饰是帕金森病早期阶段的潜在生物标志物。

IF 3.6 3区生物学

Free Radical Research Pub Date : 2024-11-01 Epub Date: 2024-11-22 DOI: 10.1080/10715762.2024.2430536

Kohei Matsuda, Yuichiro Mita, Kazumasa Saigoh, Yoshiro Saito, Noriko Noguchi

{"title":"Modifications of DJ-1 in which pI shifts to acidic in red blood cells a potential biomarker for Parkinson's disease at early stages.","authors":"Kohei Matsuda, Yuichiro Mita, Kazumasa Saigoh, Yoshiro Saito, Noriko Noguchi","doi":"10.1080/10715762.2024.2430536","DOIUrl":"10.1080/10715762.2024.2430536","url":null,"abstract":"Parkinson's disease (PD) is one of the most common neurodegenerative diseases, the incidence of which increases with age. However, since there is no fundamental treatment or methods for early diagnosis, new methods of treatment and diagnosis are urgently needed. We focused on post-translational modifications of DJ-1, which is encoded by the familial PD-causative gene PARK7 in red blood cells (RBCs). DJ-1 has three cysteines (Cys46, Cys53, and Cys106), with Cys106 being preferentially oxidized. We previously reported that sulfinated/sulfonated Cys106 DJ-1 (oxDJ-1) is increased in the RBCs of PD patients. In this study, we analyzed RBC-derived DJ-1 from PD patients and control subjects by 2-dimensional electrophoresis. We found that the ratio of the spot of DJ-1 with a more acidic isoelectric point than oxDJ-1 was increased more significantly than that of oxDJ-1 in RBCs from patients at the early stage of unmedicated PD and decreased with the progression of PD stage and treatment. Furthermore, we revealed that this acidic spot of DJ-1 increased upon exposure to H2O2. However, when either Cys53 or Cys106 of DJ-1 was replaced with serine, there was no significant increase in the acidic spot caused by H2O2. In this study, we propose a new biomarker for early diagnosis of PD using both the ratios of oxDJ-1 to total DJ-1 and the acidic spot of DJ-1 to total DJ-1.","PeriodicalId":12411,"journal":{"name":"Free Radical Research","volume":" ","pages":"748-757"},"PeriodicalIF":3.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142686456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ferritin with methylglyoxal produces reactive oxygen species but remains functional. 含有甲基乙二醛的铁蛋白会产生活性氧，但仍能发挥作用。

IF 3.6 3区生物学

Free Radical Research Pub Date : 2024-11-01 Epub Date: 2024-11-07 DOI: 10.1080/10715762.2024.2417281

Adriana Rybnikářová, Richard Buchal, Jan Pláteník

{"title":"Ferritin with methylglyoxal produces reactive oxygen species but remains functional.","authors":"Adriana Rybnikářová, Richard Buchal, Jan Pláteník","doi":"10.1080/10715762.2024.2417281","DOIUrl":"10.1080/10715762.2024.2417281","url":null,"abstract":"Iron is necessary for life, but the simultaneous iron-catalyzed formation of reactive oxygen species (ROS) is involved in pathogenesis of many diseases. One of them is diabetes mellitus, a widespread disease with severe long-term complications, including neuropathy, retinopathy, and nephropathy. Much evidence points to methylglyoxal, a potent glycating agent, as the key mediator of diabetic complications. In diabetes, there is also a peculiar dysregulation of iron homeostasis, leading to an expansion of redox-active iron. This in vitro study focuses on the interaction of methylglyoxal with ferritin, which is the main cellular protein for iron storage. Methylglyoxal effectively liberates iron from horse spleen ferritin, as well as synthetic iron cores; in both cases, it is partially mediated by superoxide. The interaction of methylglyoxal with ferritin increases the production of hydrogen peroxide, much above the generation of peroxide by methylglyoxal alone, in an iron-dependent manner. Glycation with methylglyoxal results in structural changes in ferritin. All of these findings can be demonstrated with pathophysiologically relevant (submillimolar) methylglyoxal concentrations. However, the rate of iron release by ascorbate, the ferroxidase activity, or the diameter of gated pores even in intensely glycated ferritin is not altered. In conclusion, although the functional features of ferritin resist alterations due to glycation, the interaction of methylglyoxal with ferritin liberates iron and markedly increases ROS production, both of which could enhance oxidative stress in vivo. Our findings may have implications for the pathogenesis of long-term diabetic complications, as well as for the use of ferritin as a nanocarrier in chemotherapy.","PeriodicalId":12411,"journal":{"name":"Free Radical Research","volume":" ","pages":"702-722"},"PeriodicalIF":3.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142603927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metformin suppresses metabolic dysfunction-associated fatty liver disease by ferroptosis and apoptosis via activation of oxidative stress. 二甲双胍通过激活氧化应激，抑制铁凋亡和细胞凋亡，从而抑制代谢功能障碍相关性脂肪肝。

IF 3.6 3区生物学

Free Radical Research Pub Date : 2024-11-01 Epub Date: 2024-10-18 DOI: 10.1080/10715762.2024.2417279

Zhiyu Li, Chao Cui, Liang Xu, Mingfeng Ding, Yinghui Wang

{"title":"Metformin suppresses metabolic dysfunction-associated fatty liver disease by ferroptosis and apoptosis via activation of oxidative stress.","authors":"Zhiyu Li, Chao Cui, Liang Xu, Mingfeng Ding, Yinghui Wang","doi":"10.1080/10715762.2024.2417279","DOIUrl":"10.1080/10715762.2024.2417279","url":null,"abstract":"Metformin is known for its antioxidant properties and ability to ameliorate metabolic dysfunction-associated fatty liver disease (MAFLD) and is the focus of this study. Lipoprotein-associated phospholipase A2 (Lp-PLA2) is linked to MAFLD risk. This study investigated the effects of metformin on ferroptosis in free fatty acid (FFA)-treated Huh7 hepatoma cells and its association with MAFLD risk. Using Western blot, immunofluorescence, and ELISA, this study revealed that FFA treatment led to increased intracellular fat and iron accumulation, heightened Lp-PLA2 expression, reduced levels of the cysteine transporter SLC7A11 and glutathione peroxidase 4 (GPX4), altered glutathione (GSH)/oxidized glutathione (GSSG) ratios, generation of reactive oxygen species (ROS), and initiation of lipid peroxidation, which ultimately resulted in cell ferroptosis. Importantly, metformin reversed FFA-induced iron accumulation, and this effect was attenuated by ferrostatin-1 but enhanced by erastin, RSL3, and si-GPX4. Additionally, metformin activated antioxidant and antiapoptotic mechanisms, which reduced lipid peroxidation and suppressed Lp-PLA2 expression in FFA-treated Huh7 cells. In conclusion, our findings indicate that metformin may protect against MAFLD by inhibiting iron accumulation and Lp-PLA2 expression through the ROS, ferroptosis, and apoptosis signaling pathways. This study highlights potential therapeutic strategies for managing MAFLD-related risks and emphasizes the diverse roles of metformin in maintaining hepatocyte balance.","PeriodicalId":12411,"journal":{"name":"Free Radical Research","volume":" ","pages":"686-701"},"PeriodicalIF":3.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142461846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

"Reductive stress" the overlooked side of cellular redox modulation in cancer: opportunity for design of next generation redox chemotherapeutics. 癌症细胞氧化还原调节中被忽视的 "还原应激"：设计下一代氧化还原化疗药物的机遇。

IF 3.6 3区生物学

Free Radical Research Pub Date : 2024-11-01 Epub Date: 2024-11-28 DOI: 10.1080/10715762.2024.2433988

Amit Kunwar, J Aishwarya

{"title":"\"Reductive stress\" the overlooked side of cellular redox modulation in cancer: opportunity for design of next generation redox chemotherapeutics.","authors":"Amit Kunwar, J Aishwarya","doi":"10.1080/10715762.2024.2433988","DOIUrl":"10.1080/10715762.2024.2433988","url":null,"abstract":"The last three decades of redox biology research have been dominated by the term \"oxidative stress\" since it was first coined by Helmut Sies to represent a form of cellular redox modulation characterized by redox imbalance toward overproduction of oxidants. Almost every pathological condition, including cancer, has been linked with oxidative stress and so forth; targeting oxidative stress became the strategy for the new drug discovery with anticancer drugs aiming to selectively induce oxidative stress in cancerous cells while antioxidants aiming to prevent carcinogenesis as prophylactic agents. Time has now come to realize, how harmful the other side of the cellular redox spectrum, \"reductive stress,\" characterized by redox imbalance toward the accumulation of reducing equivalents, maybe during carcinogenesis, and to tap its potential for the design of next-generation chemotherapeutic agents. Adjuvants-causing reductive stress may also work synergistically with radiation therapy under hypoxia to achieve better tumor control. Keeping this evolving field into account, the present review provides a current understating of the role of reductive stress in carcinogenesis, the status of reductive stress-based chemotherapeutic agents with particular emphasis on sulfhydryl and selenium-containing compounds and the gap areas that need to be addressed in future.","PeriodicalId":12411,"journal":{"name":"Free Radical Research","volume":" ","pages":"782-795"},"PeriodicalIF":3.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142738852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Catechol-derived Mannich bases: radical regulatory properties, cytotoxicity and interaction with biomolecules. 儿茶酚衍生曼尼希碱：自由基调节特性、细胞毒性以及与生物大分子的相互作用。

IF 3.6 3区生物学

Free Radical Research Pub Date : 2024-11-01 Epub Date: 2024-11-27 DOI: 10.1080/10715762.2024.2433985

Gvozdev M Y, Turomsha I S, Loginova N V, Varfolomeeva E Y, Kovalev R A, Fedorova N D, Ksendzova G A, Osipovich N P, Sverdlov R L

{"title":"Catechol-derived Mannich bases: radical regulatory properties, cytotoxicity and interaction with biomolecules.","authors":"Gvozdev M Y, Turomsha I S, Loginova N V, Varfolomeeva E Y, Kovalev R A, Fedorova N D, Ksendzova G A, Osipovich N P, Sverdlov R L","doi":"10.1080/10715762.2024.2433985","DOIUrl":"10.1080/10715762.2024.2433985","url":null,"abstract":"Free radicals are ubiquitous in biological systems, being responsible for pathogenesis of degenerative diseases and participating in vitally important biochemical processes, which are mediated by radical regulatory agents. The effects of the aliphatic amine substituents in the catechol-derived Mannich bases on their antioxidant and pro-oxidant activity were investigated. It has been found that the presence of catechol moiety in the structure of Mannich bases allows them to act as Cu(II) reductants, efficient Fe(II) chelators and potent DPPH radical scavengers. It has been found that the plausible mechanism of the DPPH radical scavenging proceeds via quinone formation, followed by their interaction with ethanol via the Michael addition reaction. In the neutrophil respiratory burst assay, several compounds have demonstrated a weak antioxidant activity at the micromolar level (0.1-10 µM), whereas at the millimolar level (0.1 mМ) a strong pro-oxidant effect has been observed. Additionally, at the highest used concentrations a pronounced cytotoxicity against dermal fibroblasts DF-2 and an immunosuppressive effect against T-lymphocytes have been observed for all the synthesized compounds. It has been demonstrated that the oxidation of catechols in the presence of low-molecular thiols results in the formation of covalent adducts, which provides an insight into their cytotoxicity and detoxification pathways.","PeriodicalId":12411,"journal":{"name":"Free Radical Research","volume":" ","pages":"770-781"},"PeriodicalIF":3.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142738883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Karyoptosis as a novel type of UVB-induced regulated cell death. 核衰是一种新型的uvb诱导的细胞死亡。

IF 3.6 3区生物学

Free Radical Research Pub Date : 2024-11-01 Epub Date: 2024-12-03 DOI: 10.1080/10715762.2024.2433986

Weidong Chen, Jiin Byun, Han Chang Kang, Hye Suk Lee, Joo Young Lee, Young Jik Kwon, Yong-Yeon Cho

{"title":"Karyoptosis as a novel type of UVB-induced regulated cell death.","authors":"Weidong Chen, Jiin Byun, Han Chang Kang, Hye Suk Lee, Joo Young Lee, Young Jik Kwon, Yong-Yeon Cho","doi":"10.1080/10715762.2024.2433986","DOIUrl":"10.1080/10715762.2024.2433986","url":null,"abstract":"Karyoptosis is a type of regulated cell death (RCD) characterized by explosive nuclear rupture caused by a loss of nuclear membrane integrity, resulting in the release of genomic DNA and other nuclear components into the cytosol and extracellular environment. The mechanism underlying karyoptosis involves a delicate balance between the following forces: the expansion force exerted by the tightly packed DNA in the nucleus, the resistance provided by the nuclear lamina at the inner nuclear membrane (INM), and the tensile force from the cytoskeleton that helps position the nucleus at the center of the cytoplasm, allowing it to remain maximally expanded. In addition, CREB3, a type II integral membrane protein with DNA-binding ability, tethers chromatin to the INM, providing a tightening force through chromatin interactions that prevent nuclear membrane rupture. UVB radiation can trigger this process, inducing CREB3-FL cleavage and producing CREB3-CF. Therefore, UVB acts as an intrinsic factor in the induction of karyoptosis. Importantly, biochemical analysis of RCD markers shows that karyoptosis is distinct from other forms of cell death, such as apoptosis, autophagy, necroptosis, and pyroptosis. This review explores the mechanisms involved in maintaining nuclear membrane integrity and the role of CREB3 in triggering karyoptosis and provides brief suggestions on the potential implications for targeting cancer cells.","PeriodicalId":12411,"journal":{"name":"Free Radical Research","volume":" ","pages":"796-810"},"PeriodicalIF":3.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142767705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of autophagy on the selective death of human breast cancer cells exposed to plasma-activated Ringer's lactate solution. 自噬对暴露于血浆活化林格氏乳酸溶液的人乳腺癌细胞选择性死亡的影响

IF 3.6 3区生物学

Free Radical Research Pub Date : 2024-11-01 Epub Date: 2024-12-03 DOI: 10.1080/10715762.2024.2433965

Taishi Yamakawa, Ayako Tanaka, Camelia Miron, Kae Nakamura, Hiroaki Kajiyama, Shinya Toyokuni, Masaaki Mizuno, Masaru Hori, Hiromasa Tanaka

{"title":"Effects of autophagy on the selective death of human breast cancer cells exposed to plasma-activated Ringer's lactate solution.","authors":"Taishi Yamakawa, Ayako Tanaka, Camelia Miron, Kae Nakamura, Hiroaki Kajiyama, Shinya Toyokuni, Masaaki Mizuno, Masaru Hori, Hiromasa Tanaka","doi":"10.1080/10715762.2024.2433965","DOIUrl":"10.1080/10715762.2024.2433965","url":null,"abstract":"Plasma-activated Ringer's lactate (PAL) solution prepared by irradiating an intravenous solution with a non-equilibrium atmospheric pressure plasma is a potential new cancer therapy having no side effects. However, the induction of autophagy to avoid cell death has been confirmed to occur following exposure to PAL solution. It is thought that the antitumor effect of PAL solution could be weakened by this process, which is meant to maintain homeostasis in cells and assists tumorigenesis. Thus, it would be helpful to devise PAL-based cancer therapies that inhibit autophagy. Unfortunately, it is not yet clear which substances in PAL solution promote autophagy. The present work examined the mechanism by which PAL solution induces autophagy when treating MCF-7 human breast cancer cells. Autophagy was found to be temporarily induced upon exposure to PAL solution, suggesting that this effect contributes to cell proliferation. Although autophagy is associated with reactive oxygen and nitrogen species and/or acidic environments, in this study, significant autophagy was observed using a PAL solution diluted 1/256x without these stressors. Acetate, glyoxylate and 2,3-dimethyltartrate in the PAL solution were determined to promote autophagy. Interestingly, 2,3-dimethyltartrate was found to either induce cell death or autophagy depending on the concentration.","PeriodicalId":12411,"journal":{"name":"Free Radical Research","volume":" ","pages":"758-769"},"PeriodicalIF":3.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142767793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Redox factors in the antioxidant activity of nitroxides toward DNA guanyl and 2-deoxyribose-peroxyl radicals. 硝基化合物对 DNA 鸟苷酸和 2-脱氧核糖过氧自由基的抗氧化活性中的氧化还原因素。

IF 3.6 3区生物学

Free Radical Research Pub Date : 2024-11-01 Epub Date: 2024-10-24 DOI: 10.1080/10715762.2024.2417278

Wen Qi, Smitha U Nair, David I Pattison, Robert F Anderson

{"title":"Redox factors in the antioxidant activity of nitroxides toward DNA guanyl and 2-deoxyribose-peroxyl radicals.","authors":"Wen Qi, Smitha U Nair, David I Pattison, Robert F Anderson","doi":"10.1080/10715762.2024.2417278","DOIUrl":"10.1080/10715762.2024.2417278","url":null,"abstract":"A series of eight nitroxide compounds (four substituted piperidines, three pyrrolidines and one oxo-piperidine) are found to undergo electron transfer to 2'-deoxyribose-peroxyl and the guanyl radical. One-electron oxidation potentials of the nitroxides to oxoammonium cations (oxoammonium reduction potential), E0', have been measured against a common redox indicator, chlorpromazine, and found to span the range 751 ± 15 mV to 973 ± 15 mV. Fast chemical reduction of the 2'-deoxyribose-peroxyl radical to the hydroperoxide, generated by •OH radical attack on 2-deoxyribose, dR, in oxygenated aqueous solution, is a redox-dependent reaction, with rate constants of 0.8-3.5 x 107 M-1 s-1. The guanyl radicals, produced upon one-electron oxidation of 2'-deoxyguanosine monophosphate, dG, by the selenite radical, SeO3•-, react with the nitroxides in a redox-independent reaction with diffusion rate constants of 1-2 x 108 M-1 s-1. These findings represent a possible antioxidant role for nitroxides in the fast chemical repair of DNA radicals, which is supported by an in vitro strand break study using a plasmid.","PeriodicalId":12411,"journal":{"name":"Free Radical Research","volume":" ","pages":"677-685"},"PeriodicalIF":3.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142497944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cutting-edge insights into Helicobacter research. 对螺旋杆菌研究的前沿见解。

IF 3.6 3区生物学

Free Radical Research Pub Date : 2024-10-01 Epub Date: 2024-11-06 DOI: 10.1080/10715762.2024.2421174

Hideki Mori, Hidekazu Suzuki

{"title":"Cutting-edge insights into Helicobacter research.","authors":"Hideki Mori, Hidekazu Suzuki","doi":"10.1080/10715762.2024.2421174","DOIUrl":"10.1080/10715762.2024.2421174","url":null,"abstract":"Non-Helicobacter pylori Helicobacter (NHPH) species are emerging as significant gastric pathogens. Despite their clinical importance, NHPH infections are less studied compared to Helicobacter pylori (H. pylori) due to their lower prevalence and diagnostic challenges. Zoonotic transmission, particularly from pigs, dogs, and cats, underscores the need for improved diagnostic methods and heightened clinical awareness. Gastric cancer (GC) remains a major global health issue, with H. pylori being a primary risk factor. The eradication of H. pylori reduces GC risk, but post-eradication surveillance is essential. Endoscopic findings, especially those from the Kyoto classification, and noninvasive biomarkers play crucial roles in early GC detection and risk assessment. The increasing antibiotic resistance in H. pylori necessitates new treatment strategies. Novel therapies, such as vonoprazan-based regimens, and alternatives like sitafloxacin and rifabutin, are being developed to improve eradication success rates. Understanding the fundamental mechanisms of gastric carcinogenesis, including the roles of oxidative stress and cancer stem cells, is key to advancing treatment. Targeting specific molecular pathways offers potential for more effective therapies.","PeriodicalId":12411,"journal":{"name":"Free Radical Research","volume":" ","pages":"641-647"},"PeriodicalIF":3.6,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142590261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0