Food Hydrocolloids for Health最新文献

{"title":"Green synthesis of biocompatible silver nanoparticles from Persicaria hydropiper leaves: Therapeutic and catalytic applications","authors":"Shajahan Talukder ,&nbsp;Md. Shahin Ali ,&nbsp;Sangita Kundu ,&nbsp;Md. Abdul Aziz ,&nbsp;Md. Rezaul Karim ,&nbsp;Md. Habibur Rahman ,&nbsp;M. Ahasanur Rabbi ,&nbsp;Md. Rowshanul Habib","doi":"10.1016/j.fhfh.2025.100237","DOIUrl":"10.1016/j.fhfh.2025.100237","url":null,"abstract":"<div><div>The study presents a sustainable method for synthesizing biocompatible silver nanoparticles from <em>Persicaria hydropiper</em> leaves (PH-AgNPs) with promising anti-inflammatory, antioxidant, anticancer, antimicrobial, and catalytic potentials. UV–vis analysis confirmed PH-AgNPs formation with a surface plasmon resonance (SPR) peak at 455 nm. FTIR spectra revealed the constituents of leaf extract involved in silver ion reduction. Characterization of PH-AgNPs was done using X-ray diffraction, dynamic light scattering, scanning electron microscopy, and energy dispersive X-ray techniques. This characterization confirmed that PH-AgNPs exhibited a crystalline, spherical shape with a strong silver peak. PH-AgNPs showed blood compatibility and a non-toxic nature as compared with Triton-X. PH-AgNPs exhibited moderate anti-inflammatory and antioxidant activities compared to diclofenac sodium and ascorbic acid. PH-AgNPs also showed potential as catalysts for the degradation of harmful dyes. In the anticancer experiment, PH-AgNPs treatments significantly (<em>p</em> &lt; 0.05) decreased viable EAC (Ehrlich Ascites Carcinoma) cells and lowered weight gain in mice. PH-AgNPs (<em>p</em> &lt; 0.05) restored blood counts and improved liver structure in treated mice <em>versus</em> untreated controls. Proapoptotic genes encoding p53 and Bax proteins were overexpressed in treated EAC cells, confirming the apoptotic hallmarks seen in fluorescence microscope images of these treated cells. PH-AgNPs demonstrated potent antibacterial activity, particularly against Shigella boydii, while erythromycin was the most effective against the same bacterium. PH-AgNPs combined with tetracycline showed the highest synergistic effect (37.26 %) against <em>Escherichia coli</em>. PH-AgNPs showed the lowest minimum inhibitory concentration (9.38 mg/mL) against Bacillus cereus. Therefore, the green-fabricated PH-AgNPs hold significant biological and environmental importance, offering potential for therapeutic and environmentally friendly applications.</div></div>","PeriodicalId":12385,"journal":{"name":"Food Hydrocolloids for Health","volume":"8 ","pages":"Article 100237"},"PeriodicalIF":5.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145019178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The thirst-quenching capacity of carbonated thickened drinks","authors":"Ruth Picó-Munyoz,&nbsp;Amparo Tarrega,&nbsp;Laura Laguna","doi":"10.1016/j.fhfh.2025.100226","DOIUrl":"10.1016/j.fhfh.2025.100226","url":null,"abstract":"<div><div>Thickened drinks can be prescribed to ensure the safety of individuals with swallowing difficulties. However, these beverages, especially those with starch-based thickeners, are often less appealing because of their texture and taste. Previous research has indicated that carbonation in water can improve beverage likeability and stimulate muscular activity, potentially helping in safer swallowing. This study investigates the potential of adding carbonation to thickened drinks to enhance oral hydration and alleviate dry mouth and thirst sensations. Nine different water samples prepared with or without a thickener (xanthan gum-based or starch-based) and with still or carbonated water (powder- sparkling water and sparkling commercial water) were studied. Oral moisture, dry mouth, and thirst-quenching sensations were measured before, immediately after, and 5 min after consumption. The two types of carbonation created resulted in different distribution of bubbles. Oral moisture was reduced after consuming thickened water. In addition, the thickened water, with or without carbonation, did not alleviate thirst or dry mouth. Despite the potential safety benefits of carbonation, it did not improve thirst or dry mouth sensations. Future research should explore other stimuli to enhance thirst-quenching capacity, like flavour addition to carbonation to enhance adherence to thickened beverages and body stimulation.</div></div>","PeriodicalId":12385,"journal":{"name":"Food Hydrocolloids for Health","volume":"8 ","pages":"Article 100226"},"PeriodicalIF":4.6,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144321565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent advances in plant protein microgels encapsulated with bioactive compounds","authors":"Srutee Rout ,&nbsp;Sena Bakir ,&nbsp;Sonali Khanal ,&nbsp;Gurjeet Kaur ,&nbsp;Zakir Showkat Khan ,&nbsp;Thameed Aijaz ,&nbsp;Jyoti Sharma ,&nbsp;Mohmad Sayeed Bhat ,&nbsp;Farhana Mehraj ,&nbsp;Ghazaleh Sadeghi Vahid ,&nbsp;Dinesh Kumar ,&nbsp;Prem Prakash Srivastav","doi":"10.1016/j.fhfh.2025.100238","DOIUrl":"10.1016/j.fhfh.2025.100238","url":null,"abstract":"<div><div>Protein microgels and other novel food structuring methods have garnered attention to increase plant protein functionality. Microgels, small particles (0.5–5 μm) that trap a lot of solvent, are made of a three-dimensional network of cross-linked biopolymer molecules. Protein microgels exhibit structural adaptability that makes them excellent carriers for lipophilic bioactives, protecting them from degradation and instability in aqueous systems. Additionally, their tunable design allows controlled release, enhancing bioavailability and functional efficacy in food and nutraceutical applications. The integration of scientific advances with food technology positions plant protein-based microgels as transformative tools in developing sustainable, protein-rich, and functional plant-based food systems. These advances in protein microgels prompt this review of growing plant protein sources, microgel formation, stabilization technologies, encapsulation with bioactive compounds, bioactive component transport, focusing on their functional properties, structural features, and applications. Knowing these trends can help us understand how sustainable, functioning food systems will emerge and solve global environmental and nutritional challenges.</div></div>","PeriodicalId":12385,"journal":{"name":"Food Hydrocolloids for Health","volume":"8 ","pages":"Article 100238"},"PeriodicalIF":5.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145044376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Beta-glucan-enriched diets improve immune function, antioxidant activity, and survivability in challenged oysters","authors":"Gustavo Waltzer Fehrenbach ,&nbsp;João Rui Tanoeiro ,&nbsp;Robert Pogue ,&nbsp;Frank Carter ,&nbsp;Marco F.L. Lemos ,&nbsp;Patrick Murray ,&nbsp;Declan Devine ,&nbsp;Emanuele Rezoagli ,&nbsp;Ian Major ,&nbsp;Emma J. Murphy","doi":"10.1016/j.fhfh.2025.100227","DOIUrl":"10.1016/j.fhfh.2025.100227","url":null,"abstract":"<div><div>Beta-glucans are bioactive polysaccharides with immunomodulatory and hydrocolloid properties. This study explores the potential of beta-glucans extracted from mushroom waste to enhance oyster (<em>Crassostrea gigas</em>) immunity and quality, providing a sustainable alternative to conventional aquaculture interventions. Beta-glucan supplementation led to a 100 % survival rate in treated groups compared to 70 % in the bacteria-only group (<em>p</em> = 0.024). Flow cytometry revealed a significant reduction in viable hemocytes in the beta-glucan group post-infection, suggesting modulation of inflammation. Protein content in gills, mantle, and digestive tissues increased by up to 2-fold in beta-glucan-fed oysters versus controls. Antioxidant activity, assessed by FRAP assay, was 25–40 % higher in the mantle and digestive tract if beta-glucan groups. Bacteriostatic assays demonstrated a marked reduction in <em>Vibrio</em> sp. Counts in tissues, particularly in the digestive tract, with bacterial load similar to uninfected controls. These findings highlight the dual functionality of beta-glucans as both immunomodulators and hydrocolloids, with implications for improving aquaculture sustainability and food safety. Further research should explore feeding behaviour, gut transit time, and nutrient absorption to optimize beta-glucan application in commercial shellfish production.</div></div>","PeriodicalId":12385,"journal":{"name":"Food Hydrocolloids for Health","volume":"8 ","pages":"Article 100227"},"PeriodicalIF":4.6,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144312866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Profiling oligosaccharide components in Polygonatum kingianum with potential anti-NAFLD activity using UPLC-Orbitrap-MS/MS technology","authors":"Hong Guo ,&nbsp;Rui Yao ,&nbsp;Jing Fan ,&nbsp;Ying Wang ,&nbsp;Lingzhi Zhang ,&nbsp;Hua Sun ,&nbsp;Xiaohan Guo ,&nbsp;Jianbo Yang ,&nbsp;Jingzhe Pu ,&nbsp;Yazhong Zhang ,&nbsp;Baozhong Duan ,&nbsp;Jia Chen ,&nbsp;Wenguang Jing ,&nbsp;Xianlong Cheng ,&nbsp;Feng Wei","doi":"10.1016/j.fhfh.2025.100248","DOIUrl":"10.1016/j.fhfh.2025.100248","url":null,"abstract":"<div><div>Polygonati Rhizoma serve as medicinal and edible plants rich in carbohydrates, with its polysaccharides extensively studied, while research on oligosaccharides remains limited regarding their structural and bioactive properties. This study investigates the against non-alcoholic fatty liver disease (NAFLD) effects of <em>Polygonatum kingianum</em> oligosaccharides (PKO) and employs UPLC-Orbitrap-MS/MS technology to characterize oligosaccharide components. The PKO components were enriched through hot water extraction, 60% anhydrous ethanol precipitation, and membrane separation technology, with preliminary validation of their hepatoprotective effects in DL-ethionine-induced fatty liver mice. A novel LC-MS/MS analytical method was developed utilizing characteristic secondary fragment ions and retention time profiles of oligosaccharides to enable rapid identification and characterization of PKO. Thirty-four oligosaccharides were successfully identified from <em>Polygonatum kingianum</em> (PK), all exhibiting a monosaccharide composition of fructose and glucose. The structural backbone consists of fructose residues linked via (1→2) and (2→6) glycosidic bonds, with 32 compounds being reported for the first time in PK. This study marks the first discovery of oligosaccharides in PK with potential therapeutic effects against NAFLD. Additionally, it represents the first application of LC-MS/MS technology to systematically characterize neutral oligosaccharides in this species. These findings provide a novel analytical methodology for investigating potential anti-NAFLD oligosaccharide components in PK.</div></div>","PeriodicalId":12385,"journal":{"name":"Food Hydrocolloids for Health","volume":"8 ","pages":"Article 100248"},"PeriodicalIF":5.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145320223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Encapsulation of bifidobacteria in natural biopolymers: Advances, challenges, and applications in dairy products","authors":"Marko Vinceković ,&nbsp;Lana Živković ,&nbsp;Botagoz Mutaliyeva ,&nbsp;Galiya Madybekova ,&nbsp;Elmira Turkeyeva ,&nbsp;Nenad Jalšenjak ,&nbsp;Assem Issayeva","doi":"10.1016/j.fhfh.2025.100252","DOIUrl":"10.1016/j.fhfh.2025.100252","url":null,"abstract":"<div><div>Encapsulation of <em>Bifidobacteria</em> with natural biopolymers has emerged as a promising strategy to enhance the viability of probiotics in dairy systems. While <em>Bifidobacteria</em> confer well-established health benefits, their industrial application is hindered by sensitivity to processing and storage conditions. This review critically evaluates encapsulation methods, including extrusion, emulsification, spray drying, and freeze drying, as well as natural polymers such as alginate, chitosan, carrageenan, gelatin, and starch, with a focus on their ability to maintain the stability of probiotics. Beyond summarizing current techniques, we highlight persisting gaps in scalability, cost efficiency, and consumer acceptance, issues often overlooked in earlier reviews. By integrating recent advances in material science with food engineering perspectives, this article outlines pathways for industrial translation. It distinguishes itself by focusing on how encapsulation strategies can be optimized to overcome these barriers, ultimately enabling broader adoption of probiotic-enriched dairy products.</div></div>","PeriodicalId":12385,"journal":{"name":"Food Hydrocolloids for Health","volume":"8 ","pages":"Article 100252"},"PeriodicalIF":5.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145568573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of yeast cell-wall derived from a potential probiotic yeast isolate as a controlled release agent in a triple-layer microcapsule with potent anti S. aureus activity","authors":"Sima Shams Shargh ,&nbsp;Alireza Sadeghi ,&nbsp;Mahmoud Shams Shargh ,&nbsp;Fahimeh Hajinia ,&nbsp;Ali Moayedi","doi":"10.1016/j.fhfh.2025.100241","DOIUrl":"10.1016/j.fhfh.2025.100241","url":null,"abstract":"<div><div>Application of postbiotics derived from characterized probiotics (Probio) as efficient controlled release agents of the entrapped bioactives is a promising progress in microencapsulation strategies. In the present study, phylogenetic evolutionary analysis led to the identification of <em>Pichia kudriavzevii</em> as the potential Probio yeast isolated from fermented sprouted soybeans with 82.70 % survivability under simulated gastrointestinal (SGI) conditions and potent <em>in vitro</em> inhibitory activity against <em>Staphylococcus aureus</em> (88.21 %), which was significantly (<em>p</em> &lt; 0.05) higher than those of the other foodborne bacteria tested. The yeast isolate also showed antifungal, antioxidant and suitable adhesion capabilities. Moreover, anti-ochratoxigenic activity of viable and heat-killed cells of the isolate was approved according to the HPLC-based analysis. <em>In vivo</em> safety of the isolate was also confirmed in accordance with the blood biochemistry parameters and liver enzymes activity of the fed-chicken compared to the control group. <em>In vivo</em> inhibitory activity of viable and heat-killed cells of the isolate against <em>S. aureus</em> was equal to 74.39 % and 89.71 %, respectively. Cross-linking of the yeast cell-wall derived from the isolate with the alginate-chitosan construct was also approved through FTIR and SEM analyses. In addition, antibacterial profile of the construct under SGI conditions confirmed targeted delivery and controlled release of the entrapped chitosan.</div></div>","PeriodicalId":12385,"journal":{"name":"Food Hydrocolloids for Health","volume":"8 ","pages":"Article 100241"},"PeriodicalIF":5.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145044375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pea protein and p-coumaric acid conjugates: New evidence on in vitro digestion, modulation of gut microbiota, and anti-inflammatory activity","authors":"Xuxiao Gong ,&nbsp;Jie Lin ,&nbsp;Gwoncheol Park ,&nbsp;Saurabh Kadyan ,&nbsp;Quancai Sun ,&nbsp;Ravinder Nagpal ,&nbsp;Leqi Cui","doi":"10.1016/j.fhfh.2025.100245","DOIUrl":"10.1016/j.fhfh.2025.100245","url":null,"abstract":"<div><div>This study aimed to investigate the gut health benefits and anti-inflammatory property of pea protein isolates (PPI) as affected by conjugating with p-coumaric acid (p-CA). ¹³C NMR analysis confirmed the newly formed covalent bonds between PPI and p-CA occurred at the C8 position of p-CA. Results showed that after in vitro digestion, the conjugates exhibited higher protein digestibility compared to PPI. Dialysis was conducted to simulate small intestinal absorption and obtain non-absorbable fraction, which were utilized for human fecal fermentation and for the evaluation of anti-inflammatory activity in RAW 264.7 cells. Gut microbiota analysis revealed that PPI reduced the abundance of Bifidobacterium and increased Lactobacillus. However, after conjugation with p-CA, the inhibitory effect of PPI on Bifidobacterium was diminished, while its promotive effect on Lactobacillus was further strengthened. Meanwhile, PPI exhibited anti-inflammatory potential, as evidenced by the significant suppression of nitric oxide (NO) production and IL-6 expression. While the conjugates induced higher NO production compared to PPI, the levels remained lower than those induced by LPS alone, suggesting a partial reduction in anti-inflammatory potential after conjugation. Overall, this study provides new evidence that conjugation with phenolics can enhance pea protein's potential to positively influence gut microbiota composition, albeit with a reduced anti-inflammatory potential.</div></div>","PeriodicalId":12385,"journal":{"name":"Food Hydrocolloids for Health","volume":"8 ","pages":"Article 100245"},"PeriodicalIF":5.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145216880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The action of Poria cocos polysaccharides against non-alcoholic fatty liver disease: A preliminary study","authors":"Rui Zhou ,&nbsp;Qinting Chen ,&nbsp;Jie Mo ,&nbsp;Meizhen Liu ,&nbsp;Ka Wu ,&nbsp;Chao Guo","doi":"10.1016/j.fhfh.2025.100234","DOIUrl":"10.1016/j.fhfh.2025.100234","url":null,"abstract":"<div><div>Non-alcoholic fatty liver disease (NAFLD) is a significant public health challenge owing to its current prevalence. Basic and clinical evidence indicates that pyroptosis is responsible for the development of NAFLD to non-alcoholic steatohepatitis. Our previous findings suggested that <em>Poria cocos</em> polysaccharides (PCP) exert promising anti-liver injury benefits. However, the anti-NAFLD action of PCP via the modulation of pyroptosis remains untested. In this study, a network pharmacology- and molecular docking-based approach was used to determine the anti-NAFLD activities and mechanisms of PCP prior to <em>in vitro</em> or <em>in vivo</em>. Based on these bioinformatics data, 31 intersecting genes in PCP (483 target genes), NAFLD (1445 target genes), and pyroptosis (905 target genes) were identified via network pharmacology analysis, and a total of 16 key genes in PCP against NAFLD through the modulation of pyroptosis were determined. Further enrichment analysis revealed the detailed anti-NAFLD functions and molecular mechanisms of PCP. Molecular docking simulations were used to identify the spatial binding features between PCP and the target proteins related to pyroptosis. This study determined the pyroptosis-related cluster genes that act with PCP against NAFLD, thereby offering preliminary evidence to support PCP’s potential in treating NAFLD. Additionally, we identified the possible underlying pyroptotic mechanisms for future experimental validations.</div></div>","PeriodicalId":12385,"journal":{"name":"Food Hydrocolloids for Health","volume":"8 ","pages":"Article 100234"},"PeriodicalIF":4.6,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144704139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Topical fish scale collagen cream: Formulation, skin permeation, and biocompatibility for cosmetic and therapeutic use","authors":"Ismail Zainol ,&nbsp;Ayah Rebhi Hilles ,&nbsp;Wan Rusmawati Wan Mahamod ,&nbsp;Hasnor Izzati Che Razali ,&nbsp;Haniza Hanim Mohd Zain","doi":"10.1016/j.fhfh.2025.100253","DOIUrl":"10.1016/j.fhfh.2025.100253","url":null,"abstract":"<div><div>This study aims to develop and characterise fish scale-collagen cream (CoC) intended for topical application. The development of CoC consists of hydrolysed collagen (molecular weight ≈ 1 kDa) dispersed into the aqueous phase of a water-in-oil (w/o) emulsion of Span 60: Tween 60/water/virgin coconut oil (VCO) system. Collagen used in this study has been extracted from Tilapia fish scales using hydrothermal extraction combined with enzymatic hydrolysis treatment. The CoC was developed using a water-in-VCO emulsion medium at water volume fractions (φ_w) of 0.74, 0.83, and 0.94. Up to 20 wt.% of hydrolysed collagen was successfully loaded into the emulsion system via the high shear homogenisation technique. The characterisation of the CoC involved a complex microscopic analysis, including optical polarising microscopy (OPM) and scanning electron microscopy (SEM), complemented by droplet size analysis, differential scanning calorimetry, and rheological evaluation. The physical characteristics of CoC were investigated to evaluate its suitability for administration on the skin. The potential of CoC to be applied on the skin was evaluated using analysis of <em>ex vivo</em> rat skin permeation using the Franz diffusion cell, <em>in vitro</em> fibroblast cell viability and <em>in vivo</em> toxicity to the mice. Franz diffusion cell studies revealed 3466.46 ± 21.70 μg/cm² cumulative collagen permeation over 6 h (vs. 1750 ± 319.67 μg/cm² control; <em>p</em> &lt; 0.001, 95 % CI: 1580–1850 μg/cm²), with 94–97 % retention in skin layers. <em>In vitro</em>, 0.02 mg/mL hydrolysed collagen increased fibroblast viability by 34 % (<em>p</em> &lt; 0.01, <em>n</em> = 3). Furthermore, Sub-acute toxicity tests of 25–50 mg/kg body weight of hydrolysed collagen for 7 days showed no significant weight changes (<em>p</em> &gt; 0.05, 95 % CI: −0.5 % to +1.2 %) or mortality. It was observed that the hydrolysed collagen could pass through the rat skin easily and successfully stimulate the fibroblast cell viability. In conclusion, CoC is a suitable carrier for hydrolysed collagen in topical applications. The use of the designed emulsion-based creams can safely enhance the delivery of the hydrolysed collagen in the skin layers with additional benefits for cosmetic and therapeutic applications.</div></div>","PeriodicalId":12385,"journal":{"name":"Food Hydrocolloids for Health","volume":"8 ","pages":"Article 100253"},"PeriodicalIF":5.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145568670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}