Food Hydrocolloids for Health最新文献

{"title":"Structural characterization and bioactivity of sulfated Galactan GSSG-2 from Gracilaria salicornia: Antioxidant and anticancer potential","authors":"Mythileeswari Lakshmikanthan ,&nbsp;Sakthivel Muthu ,&nbsp;Peer Mohamed Deen Mohamed ,&nbsp;Kathiravan Krishnan ,&nbsp;Nagaraj Karuppiah ,&nbsp;Ramamoorthy Karuppan ,&nbsp;Shenbhagaraman Ramalingam ,&nbsp;Anandhi Subramanian ,&nbsp;Bharathi Venkatachalam ,&nbsp;Gholamreza Abdi","doi":"10.1016/j.fhfh.2025.100236","DOIUrl":"10.1016/j.fhfh.2025.100236","url":null,"abstract":"<div><div>The sulfated galactan GSSG-2, isolated from <em>Gracilaria salicornia</em>, was investigated for its antioxidant and anticancer properties. Polysaccharides derived from marine sources are well-known for their distinctive bioactivities, making them promising candidates for pharmaceutical applications. Purification of GSSG-2 was achieved through a two-step procedure consisting of anion-exchange chromatography utilizing a DEAE-Sepharose column, followed by size-based separation via Sephadex G-100 gel filtration chromatography. Its structural features were characterized using elemental analysis, high-performance gel permeation chromatography (HPGPC), high-performance liquid chromatography (HPLC), Fourier-transform infrared spectroscopy (FT-IR), and nuclear magnetic resonance (NMR) spectroscopy. The antioxidant capacity of GSSG-2 was measured at concentrations from 25 to 150 µg/mL using assays such as DPPH radical scavenging, inhibition of superoxide anions, ferric reducing antioxidant power (FRAP), and total reducing power. In all assays, l-ascorbic acid served as the reference standard. Cytotoxic effects and induction of apoptosis in A549 human lung carcinoma cells were assessed employing MTT assays and acridine orange/ethidium bromide (AO/EtBr) fluorescence staining. GSSG-2 demonstrated a molecular weight of 70.61 kDa, with galactose identified as the dominant monosaccharide unit (328.92 mg/g). Its antioxidant activity showed a dose-dependent increase, ranging from 19.84% to 88.59%. The IC₅₀ value determined by the MTT assay was 100 µg/mL, and cell viability dropped dramatically to 16.0% at 250 µg/mL. Results from AO/EtBr staining further corroborated the time-dependent enhancement of apoptotic cell death. The strong antioxidant and anticancer effects of GSSG-2 are attributed to its structural components, notably the esterified sulfate moieties and β-(1→3)-linked d-galactopyranose units. These results underscore the potential of this compound as a therapeutic agent targeting oxidative stress and cancer.</div></div>","PeriodicalId":12385,"journal":{"name":"Food Hydrocolloids for Health","volume":"8 ","pages":"Article 100236"},"PeriodicalIF":5.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144827474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Formulation design of quercetin-loaded polyvinyl alcohol nanofibres for ocular drug delivery","authors":"Rabiah Bashir ,&nbsp;Adil Gani ,&nbsp;Asima Shah ,&nbsp;Hasham Shafi ,&nbsp;Abdul Aala Fazili ,&nbsp;Tabasum Ali ,&nbsp;Syed Naiem Raza ,&nbsp;Shabnam Kawoosa ,&nbsp;Nisar Ahmad Khan","doi":"10.1016/j.fhfh.2025.100244","DOIUrl":"10.1016/j.fhfh.2025.100244","url":null,"abstract":"<div><div>In the realm of ocular drug delivery, the limited bioavailability of pharmaceuticals presents a significant challenge. The development of alternative drug delivery systems aimed at enhancing the availability of medications at targeted sites is of considerable importance in addressing this issue. The research work aimed to design, formulate, and optimize a fast-dissolving nanofiber of quercetin and polyvinyl alcohol for an ocular delivery system. Response Surface Methodology (Box-Behnken design) was used for the optimisation of electrospinning parameters, including polymer concentration, flow rate, and voltage, resulting in enhanced drug encapsulation and the formation of smooth and uniform nanofibers. Morphological characterization was conducted using scanning electron microscopy, which demonstrated the development of smooth, uniform, bead-like porous fibers with the drug incorporated within the fibers in nanoform during the electrospinning process, rather than being deposited on the surface. The drug-excipient interaction was evaluated by FTIR analysis, which indicated compatibility between PVA and quercetin, with all the peaks of the quercetin retained in the optimized formulation. The conversion of quercetin from its crystalline form to an amorphous state was demonstrated through X-ray diffraction (XRD) and differential scanning calorimetry (DSC) analyses. The XRD pattern illustrated a reduction in the crystalline peaks characteristic of quercetin, while the DSC results indicated a decrease in the melting point of quercetin in nanofibers. The physical, chemical, and pharmaceutical characteristics of the optimized nanofibre formulation were appropriate and within limits. Disintegration was accomplished within 45 s, with approximately 100% of the active ingredient released within 10 min during an in vitro dissolution test. In contrast, the conventional eye drop formulation exhibited a release of only 50%. Eye irritation study results indicated that there was no visual sign of irritation in all experimental rabbits. The optimized formulation underwent an accelerated stability study and retained its original properties with negligible changes. There was no significant alteration in physical appearance, flexibility, disintegration time, or drug encapsulation, confirming its stability.</div></div>","PeriodicalId":12385,"journal":{"name":"Food Hydrocolloids for Health","volume":"8 ","pages":"Article 100244"},"PeriodicalIF":5.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145216285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preserving and hydrogel-matrixing the bioactive properties of aromatic medicinal halophytic herbs from the coastline of the Iberian Peninsula","authors":"Tiago Parracho ,&nbsp;Pedro F. Cruz ,&nbsp;Claúdia C. Peralta ,&nbsp;Cândida G. Silva ,&nbsp;Maria Jorge Campos ,&nbsp;Marta Neves ,&nbsp;Rachel Cordeiro ,&nbsp;Daniela Trindade ,&nbsp;Carla Moura ,&nbsp;Zaida L. Almeida ,&nbsp;Cidália D. Pereira ,&nbsp;Carla Guimarães ,&nbsp;Rui M.M. Brito ,&nbsp;Mauro Guerra ,&nbsp;Fernando Reboredo ,&nbsp;Paula Veríssimo ,&nbsp;Vânia Ribeiro ,&nbsp;Daniela C. Vaz","doi":"10.1016/j.fhfh.2025.100239","DOIUrl":"10.1016/j.fhfh.2025.100239","url":null,"abstract":"<div><div>Edible wild plants are part of the ethnobotanic heritage of a certain geographical area and are important sources of essential oils, antioxidants, minerals, and special flavours. <em>Corema album</em> (Portuguese crowberry), <em>Crithmum maritimum</em> (sea fennel), <em>Eryngium maritimum</em> (sea holly), <em>Helichrysum italicum</em> (curry plant) and <em>Otanthus maritimus</em> (cottonweed) wildly flourish along the sandy dunes of the coast of the Iberian Peninsula. These plants are locally known for their beneficial properties, with important value for food, cosmetics and/or medicinal applications. Hence, leaves of these endemic species were collected at four different locations and submitted to different preserving treatments (oven-drying, freezing, and freeze-drying). Acetonic extracts of the different plants submitted to the different post-harvesting treatments were analysed regarding their antioxidant capacities and phenolic contents. Plant extracts were also analysed by diffusion-ordered nuclear magnetic resonance spectroscopy (DOSY-NMR). In general, freeze-drying was the best method of preserving plant minerals, antioxidants (∼4 mgVCEAC/g fw) and polyphenols (∼5 mgGAE/g fw). Minerals were quantified via energy-dispersive X-ray fluorescence spectrometry, and despite their location, all plants were rich in Ca, Cl, K, S and P. Hierarchical clustering and principal component analyses (PCA) pointed towards chemical/metabolic proximity between taxonomic families. Alginate hydrogels loaded with 0.1 % and 0.2 % (w/v) of extracts presented homogenous surface properties by scanning electron microscopy, good mechanical tensile strength (∼30 MPa) and antibacterial activity against <em>S. aureus</em>. Edible alginate hydrogels enriched with plant extracts hold great nutraceutical potential to be used as natural preservatives for food coating and packaging or as sources of bioactive compounds for biomedical applications.</div></div>","PeriodicalId":12385,"journal":{"name":"Food Hydrocolloids for Health","volume":"8 ","pages":"Article 100239"},"PeriodicalIF":5.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145026979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Moringa Oleifera as a multifunctional bioresource: Nutritional, medicinal and its pickering emulsifying potential","authors":"Namrah Azmi ,&nbsp;Andrew Akanno ,&nbsp;Charlotte Jacobsen ,&nbsp;Jon Otto Fossum","doi":"10.1016/j.fhfh.2025.100231","DOIUrl":"10.1016/j.fhfh.2025.100231","url":null,"abstract":"<div><div><em>Moringa Oleifera</em> is a versatile plant that has garnered considerable attention for its diverse applications. The nutritional content and bioactive compounds present in <em>M. oleifera</em> seeds and leaves make it a promising candidate for use in the food and pharmaceutical industries. While numerous traditional and scientific claims exist regarding its benefits, many remain unsubstantiated. Therefore, a critical and comprehensive evaluation of the available literature is essential for advancing the development of valuable applications. This review examines the reported economic significance of <em>M. oleifera</em> across various sectors, traditional uses across regions, and its nutritional and medicinal potential. Particular attention is given to its application in Pickering emulsions, emphasizing its capacity to serve as a sustainable and bio-based emulsifier. The review also explores the influence of extraction methods on protein functionality and highlights future prospects for incorporating <em>M. oleifera</em>-based emulsifiers in food, cosmetic, and pharmaceutical formulations.</div></div>","PeriodicalId":12385,"journal":{"name":"Food Hydrocolloids for Health","volume":"8 ","pages":"Article 100231"},"PeriodicalIF":4.6,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144695294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Proteins derived from green biomass: Alfalfa (Medicago sativa L.) and water lentil concentrate (Lemna minor L.) in the focus as stabilizers for emulsions","authors":"Maren Müller ,&nbsp;Olaf Holderer ,&nbsp;Kuno Schwärzer ,&nbsp;Anika Wiese-Klinkenberg ,&nbsp;Beate Förster ,&nbsp;Stephan Förster ,&nbsp;Joachim Kohlbrecher ,&nbsp;Kathleen Wood ,&nbsp;Baohu Wu ,&nbsp;Stephan Hauschild ,&nbsp;Henrich Frielinghaus ,&nbsp;Theresia Heiden-Hecht","doi":"10.1016/j.fhfh.2025.100233","DOIUrl":"10.1016/j.fhfh.2025.100233","url":null,"abstract":"<div><div>Green crop biomass is a sustainable protein source, which can support solving global food challenges as well as improving the bioeconomy for novel food utilization. Two green biomasses -alfalfa and water lentil- known for a high protein content and their value as feed and food components were investigated for their potential in stabilizing emulsions.</div><div>For interfaces in complex food systems like emulsions, the proteins from the green biomasses act similarly to other plant proteins and even to some extent to whey proteins. The extracted green biomass protein concentrates were composed of a diverse mixture of proteins: RuBisCo as the main component, enzymes like ATP subunit synthase, chlorophyll a-b binding proteins, histones and cytochromes, which all were associated as protein aggregates or protein-polyphenol complexes with sizes from 33 up to 85 nm.</div><div>These multicomponent and complex green protein components were obtained in an extraction process, and proved their capability to stabilize emulsions. Key parameters are a narrow oil droplet size distribution with rather small oil droplets with a median of about 3.5 μm, a low interfacial tension with around 15 mN/m and an elastic interfacial layer with an elastic modulus of about 45 mN/m comparable to functional whey or plant proteins. The oil/water interface of the emulsion revealed a more pronounced structural rearrangement for alfalfa protein compared to water lentil proteins as quantified by small-angle neutron scattering (SANS), indicating the relevance of plant protein source to the final structure.</div><div>Green biomass proteins from alfalfa and water lentil represent a novel protein source to stabilize emulsions.</div></div>","PeriodicalId":12385,"journal":{"name":"Food Hydrocolloids for Health","volume":"8 ","pages":"Article 100233"},"PeriodicalIF":5.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144780239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization and physicochemical properties of a novel microencapsulated bio-calcium from Asian sea bass bones","authors":"Salinee Phengleng ,&nbsp;Wipavadee Sangadkit ,&nbsp;Songsak Wattanachaisaereekul ,&nbsp;Jiraporn Sirison ,&nbsp;Suneerat Ruangsomboon","doi":"10.1016/j.fhfh.2025.100247","DOIUrl":"10.1016/j.fhfh.2025.100247","url":null,"abstract":"<div><div>Calcium is the most abundant mineral in the human body, yet intake remains insufficient in many populations. Fishbone-derived bio-calcium from Asian sea bass (<em>Lates calcarifer</em>), containing approximately 37.5 % calcium (dry weight), offers a cost-effective source. However, its primary form, hydroxyapatite, has low solubility due to high crystallinity, limiting its application in food fortification. This study aimed to enhance the physicochemical properties of bio-calcium (B) powders by encapsulating them with maltodextrin (M), gum arabic (G), and their combination (MG) at 5 %, 10 %, and 15 % (w/v) using spray drying. A 1:4 (w/w) ratio of B to wall materials was applied at 180 °C (inlet) and 60 °C (outlet) temperatures. Powder yields ranged from 25.2 % (15 % BG) to 30.3 % (15 % BM), with no significant differences (<em>p</em> &gt; 0.05) among treatments. Encapsulated powders had higher lightness (L*) than B. The highest calcium content and encapsulation efficiency were observed in 5 % BG, while BM showed the lowest. Moisture content and water activity remained below 10 % and 0.6 %, respectively. BG had the highest hygroscopicity, while wall concentration had no significant (p &gt; 0.05) impact. Encapsulation improved water solubility index (75.4–86.5 %), especially in BM. Particle sizes ranged from 0.92 µm (10 % BMG) to 2.89 µm (15 % BM), while zeta potentials ranged from -8.71 mV (15 % BM) to -20.90 mV (15 % BMG). Encapsulated powders were more spherical and smoother than B, while BG particles showed aggregation, whereas BMG showed mixed morphologies. These findings suggest that encapsulation enhanced the physicochemical properties of bio-calcium, supporting its potential application in calcium-fortified foods and dietary supplements.</div></div>","PeriodicalId":12385,"journal":{"name":"Food Hydrocolloids for Health","volume":"8 ","pages":"Article 100247"},"PeriodicalIF":5.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145320229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of oral probiotic supplementation on gut permeability in obesity: A systematic review of randomized controlled trials","authors":"Alpha Fardah Athiyyah ,&nbsp;Triska Susila Nindya ,&nbsp;Reza Gunadi Ranuh ,&nbsp;Andy Darma ,&nbsp;Khadijah Rizky Sumitro ,&nbsp;Purwo Sri Rejeki ,&nbsp;Lilik Djuari ,&nbsp;Subijanto Marto Sudarmo ,&nbsp;Rina Agustina","doi":"10.1016/j.fhfh.2025.100225","DOIUrl":"10.1016/j.fhfh.2025.100225","url":null,"abstract":"<div><h3>Background</h3><div>Obesity is a chronic condition and a global epidemic, associated with impaired intestinal permeability owing to dysbiosis. Gut microbiota reportedly plays a potential role in obesity treatment. Probiotics have emerged as the most widely used approach for treating dysbiosis. This study explored the effect of oral probiotic supplementation on intestinal permeability in individuals with obesity.</div></div><div><h3>Methods</h3><div>A Systematic review was conducted in accordance with Preferred Reporting Items for Systematic Reviews and Meta-Analyses. PubMed, ScienceDirect, PLOS One, and Cochrane databases were searched to identify articles published between 2014 and September 2024. The Cochrane risk-of-bias tool for randomized trials was used to assess publication bias.</div></div><div><h3>Results</h3><div>A total of 690 human RCTs (randomized controlled trials) were identified, of which nine were eligible for this review. Probiotics (<em>Akkermansia, Bifidobacterium, Enterococcus, Lactobacillus, Streptococcus,</em> and a combination of <em>Saccharomyces</em> yeast) used for over 8 weeks may decrease gut permeability outcomes, including plasma zonulin, fecal zonulin, plasma or serum LPS, urinary lactulose, and urinary mannitol; however, BMI (body mass index) levels exhibited inconsistent measurements across all groups.</div></div><div><h3>Conclusions</h3><div>Probiotic supplementation for a minimum of 8 weeks may reduce gut permeability. However, further studies are required to develop standardized protocols for probiotic strains, dosages, durations, and delivery methods, to better understand their effects on intestinal permeability and related outcomes in individuals with obesity.</div></div>","PeriodicalId":12385,"journal":{"name":"Food Hydrocolloids for Health","volume":"8 ","pages":"Article 100225"},"PeriodicalIF":4.6,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144291447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced oral delivery of zeaxanthin via chitosan/alginate nanoparticles: Optimizing stability and antioxidant efficacy in retinal cells","authors":"Feuangthit Niyamissara Sorasitthiyanukarn ,&nbsp;Chawanphat Muangnoi ,&nbsp;Nonthaneth Nalinratana ,&nbsp;Pranee Rojsitthisak ,&nbsp;Pornchai Rojsitthisak","doi":"10.1016/j.fhfh.2025.100254","DOIUrl":"10.1016/j.fhfh.2025.100254","url":null,"abstract":"<div><div>Zeaxanthin (ZT), a lipophilic carotenoid with strong antioxidant potential, suffers from poor aqueous solubility and low oral bioavailability, which limits its therapeutic application. In this study, chitosan/alginate nanoparticles (CS/ALG-NPs) were developed and optimized for oral ZT delivery using a Box-Behnken design. The optimized NPs showed a particle size of 268 ± 35 nm, a zeta potential of −25.2 ± 0.8 mV, and an encapsulation efficiency of 75.4 ± 3.4%. <em>In vitro</em> release under simulated gastrointestinal (GI) conditions exhibited sustained release with improved digestive stability and bioaccessibility compared to free ZT. The optimized ZT-CS/ALG-NPs exhibited favorable stability, maintaining particle size, surface charge, and encapsulation efficiency during storage at 4 °C, and retained their spherical morphology and uniform dispersion after simulated GI digestion, supporting their potential for oral delivery applications. In ARPE-19 cells, ZT-CS/ALG-NPs significantly reduced intracellular reactive oxygen species, restored antioxidant enzyme activities (SOD, CAT, GPx), and increased intracellular glutathione (GSH) levels compared to free ZT. Under H₂O₂-induced oxidative stress, ZT-CS/ALG-NPs reduced the expression of pro-apoptotic proteins (Bax and cytochrome c) and increased the expression of anti-apoptotic protein (Bcl-2) in ARPE-19 cells (<em>vs</em> H₂O₂ group). Transmission electron microscopy and flow cytometry confirmed cellular uptake. These findings demonstrate the potential of CS/ALG-NPs as a polysaccharide-based oral delivery system to enhance the stability, bioaccessibility, and antioxidant efficacy of hydrophobic bioactives such as zeaxanthin.</div></div>","PeriodicalId":12385,"journal":{"name":"Food Hydrocolloids for Health","volume":"8 ","pages":"Article 100254"},"PeriodicalIF":5.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145614355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances in food-grade hydrogel encapsulation of probiotics with next-generation prebiotics for targeted synbiotic delivery","authors":"Vidmantė Minelgaitė,&nbsp;Sigita Jeznienė,&nbsp;Aušra Šipailienė","doi":"10.1016/j.fhfh.2025.100251","DOIUrl":"10.1016/j.fhfh.2025.100251","url":null,"abstract":"<div><div>Background. Hydrogel-based encapsulation represents a successful method for preserving probiotics and their functionality during processing, storage, and gastrointestinal transit. Although conventional systems offer protection, advances in next-generation prebiotics such as polyphenols and non-digestible carbohydrates have shifted the focus toward multifunctional synbiotic delivery systems. These materials provide sustainable, food-grade design and improve both structural integrity and bioactivity. Additionally, fabrication and crosslinking methods such as ionic interactions, Maillard conjugation, and enzymatic or pH-responsive techniques facilitate precise hydrogel customization for targeted probiotic release. Scope and approach. This review discusses recent progress in natural and composite hydrogels for probiotic encapsulation, with particular attention to the influence of next generation prebiotics on hydrogel functionality. A decision-oriented design framework is presented, aligning target sites and release triggers with materials and crosslinking strategies, exemplified with quantitative results. This framework offers a systematic approach for selecting food-grade matrices and encapsulation methods. Key findings and conclusions. Natural and composite hydrogels provide effective protection against oxygen, heat, acidity, bile salts, and digestive enzymes, maintaining a hydrated and biocompatible microenvironment. Protein–polysaccharide combinations increase mechanical and rheological stability, while next-generation prebiotics further reinforce structural integrity and bioactivity. Encapsulation efficiencies above 90 percent and enhanced cell viability in simulated digestion demonstrate promising performance of these systems. The integration of quantitative mapping and design principles establishes a practical framework for developing scalable, food-grade, multifunctional synbiotic hydrogels, supporting advancements in probiotic delivery technology. Adopting standardized digestion models and prioritizing in vivo validation will aid the development of synbiotic hydrogels acceptable in real food systems.</div></div>","PeriodicalId":12385,"journal":{"name":"Food Hydrocolloids for Health","volume":"8 ","pages":"Article 100251"},"PeriodicalIF":5.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145412510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extraction, purification, structure characterization of polysaccharides from Cistanche deserticola and their biological effects","authors":"Faqin Tao ,&nbsp;Baotang Zhao ,&nbsp;Shen Song ,&nbsp;Yunfei Xu ,&nbsp;Ji Zhang","doi":"10.1016/j.fhfh.2025.100235","DOIUrl":"10.1016/j.fhfh.2025.100235","url":null,"abstract":"<div><div>The polysaccharide fraction (CDP-D2-N1) from the stem of Cristanche deseticola was obtained after extraction and purification. The structural characteristics and in vitro antioxidant and anti-inflammatory activities of CDP-D2-N1 were investigated by physical, chemical and instrumental analyses. The results showed that CDP-D2-N1 (127.784 kDa) is a heterogeneous polysaccharide with an irregular non-crystalline structure, containing mainly Rha:Ara:Xyl:Gal:Gal:GlcA:Glc:GalA, and a molar ratio of 6.75 : 26.68 : 1.56 : 37.87 : 3.94 : 2.17 : 21.02. Combined with the results of NMR spectroscopy and methylation analyses, the main chain of CDP-D2-N1 was determined to be →4)-α-<span>d</span>-GalpA-(1→,→3,6)-β-<span>d</span>-Galp-(1→, →5)-α-<span>l</span>-Araf-(1→, containing a small amount of fragment →3,4)-β-<span>d</span>-Galp-(1→ and →3)-β-<span>d</span>-Galp-(1→, and the CDP-D2-N1 structure was inferred. In vitro assays demonstrated that CDP-D2-N1 possessed significant antioxidant capacity and attenuated inflammation induced by lipopolysaccharide (LPS) from <em>Escherichia coli</em> O55:B5 in bovine endothelial cells (bEEcs) by inhibiting the expression levels of TNF-α, IL-6, TLR 4 and IL-1.</div></div>","PeriodicalId":12385,"journal":{"name":"Food Hydrocolloids for Health","volume":"8 ","pages":"Article 100235"},"PeriodicalIF":5.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144780240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}