Food Hydrocolloids for Health最新文献

{"title":"Impact of partial enzymatic hydrolysis on the transport and uptake of SPI nanoparticles post-digestion","authors":"Ling Chen ,&nbsp;Yuan Lv ,&nbsp;Xiaoyong Wang ,&nbsp;Maoshen Chen ,&nbsp;Hui Zhang","doi":"10.1016/j.fhfh.2025.100223","DOIUrl":"10.1016/j.fhfh.2025.100223","url":null,"abstract":"<div><div>Despite extensive research on protein-based nanoparticles for nutrient delivery, the structural features that govern bile salt interactions and subsequently enhance intestinal absorption and metabolic transformation remain insufficiently clarified. In this study, soy protein isolate nanoparticles enzymatically modified by Alcalase, Neutrase, and Flavorzyme (SPIH-A@NP, SPIH<img>N@NP, SPIH-F@NP) were constructed as structural models to explore the relationship between nanoparticle interfacial properties, bile salt adsorption, and nutrient transport efficiency, using simulated gastrointestinal digestion and Caco-2/HT29 co-culture models. Compared with undigested nanoparticles and free <em>β</em>-carotene, digested nanoparticles exhibited significantly improved cellular uptake and transport efficiencies. Among them, SPIH<img>N@NP and SPIH-F@NP retained more hydrophobic peptides and acidic subunits from 11S globulin, which enhanced bile salt adsorption, maintained smaller particle sizes post-digestion, and facilitated more efficient delivery of <em>β</em>-carotene and its metabolites. Additionally, the digestion process altered uptake pathways, with macropinocytosis contributing more prominently post-digestion. These findings provide mechanistic insight into structure–function relationships that can guide the rational design of protein-based nanocarriers for targeted absorption and metabolic modulation of lipophilic nutrients.</div></div>","PeriodicalId":12385,"journal":{"name":"Food Hydrocolloids for Health","volume":"7 ","pages":"Article 100223"},"PeriodicalIF":4.6,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144178209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a self-nanoemulsifying system for the oil extract of Mentha spicata L. and evaluation of its anticancer efficacy in vitro","authors":"F. Morovat ,&nbsp;M. Karimi ,&nbsp;R. Dehdari Vais ,&nbsp;M. Negahdary ,&nbsp;S.A. Dastgheib ,&nbsp;H. Heli","doi":"10.1016/j.fhfh.2025.100222","DOIUrl":"10.1016/j.fhfh.2025.100222","url":null,"abstract":"<div><div>Nanocarriers based on (natural) lipids have been extensively studied to improve the oral bioavailability of anticancer compounds/drugs with poor water solubility. Self-nanoemulsifying drug delivery systems (SNEDDSs) are a route to improve the solubility and bioavailability of lipophilic compounds. In a present study, an SNEDDS using the oil extract of <em>Mentha spicata</em> L. leaves, Tween 80, and PEG 600 (SMO) was prepared. Pseudo-ternary phase diagrams were constructed to find suitable surfactant, solubilizer with the oil extract that form self-nanoemulsion after dilution in water. SMO comprised Tween 80:PEG 600:the oil with 2:3:5 in volume ratios or 2.1:3.4:4.5 in mass ratios. It comprised nanodroplets of 82.5 ± 4.7 nm in diameter and had a stability of &gt;45 days. SMO led to enhanced cytotoxicity of the oil extract with IC-50 values of 1.9 ± 0.1 and 2.0 ± 0.1 mg mL⁻¹ for MDA-MB-231 and MCF-7 cells, respectively. SMO would be applicable for delivery of either water insoluble chemotherapeutic drugs or natural anticancer oils upon more investigations.</div></div>","PeriodicalId":12385,"journal":{"name":"Food Hydrocolloids for Health","volume":"7 ","pages":"Article 100222"},"PeriodicalIF":4.6,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144147172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimization of phenolic extraction method and in vitro bioaccessibility of microencapsulated pigmented rice bran extracts and their antioxidant and anticancer properties","authors":"Rhowell Navarro Tiozon Jr. ,&nbsp;Glenn Vincent P. Ong ,&nbsp;Kristel June D. Sartagoda ,&nbsp;Sheba Mae M. Duque ,&nbsp;Saleh Alseekh ,&nbsp;Aldrin P. Bonto ,&nbsp;Shem Gempesaw Jr. ,&nbsp;Vipin Pratap ,&nbsp;Florencio C. Reginio Jr. ,&nbsp;Jonina Marie J. Tengco ,&nbsp;Christian Seagan ,&nbsp;Joel H G Tolentino ,&nbsp;Dennis Marvin O. Santiago ,&nbsp;Alisdair R. Fernie ,&nbsp;Nese Sreenivasulu","doi":"10.1016/j.fhfh.2025.100221","DOIUrl":"10.1016/j.fhfh.2025.100221","url":null,"abstract":"<div><div>Pigmented paddy rice is rich in a diverse array of phytochemicals that confer notable antioxidant and anticancer properties. However, the stability and bioaccessibility of these bioactive compounds present significant challenges. In this study, 542 brown (including pigmented and non-pigmented) whole grain rice samples were screened for their antioxidant components and capacity, leading to the identification of three superior cultivars: Balatinao variable purple rice, Ketan Hitam variable purple rice, and Kintuman red rice. Using response surface methodology, rice bran extracts from these cultivars were subjected to microencapsulation to stabilize the phytochemicals. Among the microencapsulated rice bran extracts (MRBEs), Ketan Hitam MRBE demonstrated significantly higher total phenolic content (TPC) and antioxidant capacity. This enhancement is likely due to the increased concentrations of isovitexin, luteolin 7-glucoside, and vitexin following microencapsulation. Furthermore, compared to non-encapsulated rice bran extracts, MRBEs exhibited significantly improved anticancer activity against HCT116 (colon) and A549 (lung) cancer cell lines (<em>P</em> &lt; 0.05). Subsequent fractionation of the MRBE allowed for the identification of the most bioactive fractions, which contained metabolites effective against these cancer cell lines. In addition, <em>in vitro</em> bioaccessibility assays revealed a controlled release of 19 targeted phenolic compounds. This release profile was characterized by an initial increase during the gastric digestion phase, followed by a decrease in the intestinal phase. Notably, phenolic compounds such as chlorogenic acid, gallic acid, and vanillin were preserved across the three rice varieties after microencapsulation. These findings underscore the potential of MRBEs as functional food ingredients or supplements, offering improved bioaccessibility of phenolics, enhanced antioxidant properties, and promising anticancer activity. The results support the integration of rice bran extracts into the rice value chain, promoting their use in functional health applications.</div></div>","PeriodicalId":12385,"journal":{"name":"Food Hydrocolloids for Health","volume":"7 ","pages":"Article 100221"},"PeriodicalIF":4.6,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144130942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Formulation optimization of orodispersible film containing essential oil from fruits of Zanthoxylum rhetsa (Roxb.) DC. using response surface methodology and evaluation of its antioxidant, and antiglycation activities","authors":"Pimpikar Kanchanadumkerng ,&nbsp;Vilasinee Hirunpanich Sato ,&nbsp;Nattawut Charoenthai ,&nbsp;Thongtham Suksawat ,&nbsp;Pattamapan Lomarat ,&nbsp;Savita Chewchinda","doi":"10.1016/j.fhfh.2025.100220","DOIUrl":"10.1016/j.fhfh.2025.100220","url":null,"abstract":"<div><div>This study aimed to develop an optimal formulation for an orodispersible film containing essential oil from <em>Zanthoxylum rhetsa</em> (ZR film) using response surface methodology with a Box-Behnken design experiment and to evaluate its antioxidant and antiglycation activities. Three independent variables—gelatin (1–3 % w/v), carrageenan (0.5–1.5 % w/v), and essential oil from <em>Z. rhetsa</em> fruits (1–3 % w/v)—were utilized to design a ZR film with desirable characteristics. Dependent variables, including film thickness, moisture content, and disintegration time, were assessed. The optimized formulation contained 1 % w/v gelatin, 0.5 % w/v carrageenan, and 2.6 % w/v essential oil from <em>Z. rhetsa</em> fruits. The optimized ZR film exhibited a thickness of 14.2 ± 2.12 µm, a disintegration time of 6.34 ± 0.53 min, and a moisture content of 3.32 ± 0.56 %. Total phenolic content was 55.3 ± 0.4 mg GAE/g, and ABTS radical scavenging capacity was 0.06 ± 0.02 g TEAC/g. Additionally, the ZR film inhibited the formation of advanced glycation end products (AGEs) by 66.97 ± 0.58 %. This ZR film shows promise as a potential oral healthcare product for managing oxidative stress and glycation-related pathologies.</div></div>","PeriodicalId":12385,"journal":{"name":"Food Hydrocolloids for Health","volume":"7 ","pages":"Article 100220"},"PeriodicalIF":4.6,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144106644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of sodium chloride microcapsules as saltiness enhancement strategy","authors":"Juan Carlos SOLOMANDO,&nbsp;Teresa ANTEQUERA,&nbsp;Abraham Pajuelo,&nbsp;Jorge RUIZ-CARRASCAL,&nbsp;Trinidad PEREZ-PALACIOS","doi":"10.1016/j.fhfh.2025.100219","DOIUrl":"10.1016/j.fhfh.2025.100219","url":null,"abstract":"<div><div>This study aimed to develop salt microcapsules using different wall materials to achieve stable encapsulated salt systems with optimal water solubilization and controlled salt release in the oral cavity, leading to an acceptable saltiness perception in food matrices. Six types of salt microcapsules were prepared, uncoated salt (CONT) and coated with maltodextrin (MALT), chitosan (QUIT), double emulsion (DOBL), alginate (ALGI), and liposomes (LIPO).</div><div>Significant differences were observed in size distribution, intermolecular forces, solubility index, salt release during oral digestion, and sensory evaluation. MALT microcapsules exhibited the most homogeneous size distribution, while ALGI showed the smallest particle size (D(4.3) = 0.27 μm). QUIT and DOBL microcapsules demonstrated the strongest intermolecular interactions between Na+ and Cl− ions and their respective wall materials, giving the lowest solubility (77.95 and 58.74 %) and oral salt release values (39.51 and 45.87 %). Contrarily, CONT, MALT, ALGI, and LIPO microcapsules exhibited higher solubility (99.77, 90.33, 92.65 and 86.01 %) and salt release (96.62, 83.08, 88.22 and 86.01 %).</div><div>Sensory analysis revealed that coated salt microcapsules enhanced the perception of saltiness compared to uncoated samples, demonstrating the potential of salt encapsulation as a strategy to reduce overall sodium content in foods without compromising taste. However, QUIT and ALGI microcapsules received the lowest hedonic scores, possibly due to undesirable sensory attributes associated with their wall materials.</div><div>Overall, MALT, DOBL, and LIPO microcapsules emerged as the most promising formulations, offering a balance of favorable characteristics across multiple parameters and highlighting their potential application in the development of reduced-sodium food products.</div></div>","PeriodicalId":12385,"journal":{"name":"Food Hydrocolloids for Health","volume":"7 ","pages":"Article 100219"},"PeriodicalIF":4.6,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144195961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lactobacillus plantarum in high methoxylated pectin-based encapsulation systems supports gut epithelial wound healing in vitro","authors":"Felipe Galvez-Jiron ,&nbsp;Lucas de Freitas Pedrosa ,&nbsp;Luis Silva Lagos ,&nbsp;Xin Tang ,&nbsp;Traudy Wandersleben ,&nbsp;Rodrigo Navia ,&nbsp;Stephan Drusch ,&nbsp;Francisca Acevedo ,&nbsp;Paul de Vos","doi":"10.1016/j.fhfh.2025.100218","DOIUrl":"10.1016/j.fhfh.2025.100218","url":null,"abstract":"<div><div>Wound healing and gut epithelial barrier regulation are crucial for intestinal homeostasis. Effective repair of gut epithelial wounds is crucial for re-establishing the mucosal barrier and resolving inflammation. Pectins, versatile polysaccharides in food, protect sensitive components, such as living bacteria, during gastrointestinal transit and support wound healing by promoting cell proliferation and migration.</div><div>This study investigated the effects of encapsulated <em>Lactobacillus plantarum WCFS1</em> on cell proliferation and migration during epithelial wound healing, using pectin-based beads stabilized with alginate or chitosan. Pectin from lemon, with two degrees of methyl esterification (DM18 and DM88), produced four microbead types. T84 cells were incubated with these beads for 24 hours. The results revealed that cell proliferation and migration were influenced by the bead type, the degree of methyl esterification of the pectin, and the inclusion of <em>L. plantarum</em>. Empty DM88 pectin beads improved cell proliferation while reducing migration tendencies, whereas DM18 beads delayed migration.Beads containing <em>L. plantarum</em> demonstrated different effects: DM18 beads enhanced proliferation without inhibiting migration, while DM88 beads reduced migration. Both empty and <em>L. plantarum</em>-loaded beads decreased CCL20 production. Alginate/pectin beads with <em>L. plantarum</em> increased IL-8, while chitosan/pectin beads delayed migration, reduced CCL20 secretion, and modulated IL-8 levels.</div><div>In conclusion, DM88 pectin beads without <em>L. plantarum</em> and DM18 beads containing <em>L. plantarum</em> supported cell proliferation over migration during epithelial wound healing. These findings suggest that pectin-based encapsulation systems may play a role in promoting gut health, protecting barrier integrity, and delivering therapeutic agents.</div></div>","PeriodicalId":12385,"journal":{"name":"Food Hydrocolloids for Health","volume":"7 ","pages":"Article 100218"},"PeriodicalIF":4.6,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144084373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Beneficial effects of soluble dietary fiber from Dendrocalamus brandisii Munro shoots on high-fat diet-induced metabolic disorders in mice: Impact on liver, adipose tissue, and intestinal health","authors":"Mingtian Tian ,&nbsp;Jiahong Dong ,&nbsp;Yuhong Guo ,&nbsp;Yihe Zhao ,&nbsp;Jianxin Cao","doi":"10.1016/j.fhfh.2025.100217","DOIUrl":"10.1016/j.fhfh.2025.100217","url":null,"abstract":"<div><div>Bamboo shoot soluble dietary fiber (DBS-SDF) has numerous health-promoting benefits, but its protective effects and potential mechanism against obesity-related metabolic disorders remain unclear. The effects of DBS-SDF on metabolic disorders induced by a high-fat diet (HFD) in mice was studied. Supplementation with DBS-SDF alleviated the development of HFD-induced obesity and related symptoms by lowering serum lipid levels, preventing lipid degeneration, and modulating the expression levels of genes related to lipid synthesis and decomposition in the liver. At the same time, DBS-SDF reversed the enlargement of white adipose cells, prevented the whitening of brown adipose tissue, and activated fat browning to improve energy balance. In addition, DBS-SDF reshaped the structure of the gut microbiota, significantly reducing the ratio of Firmicutes/Bacteroidetes, promoting the proliferation of SCFA producing bacteria <em>Ligilactobacillus</em>, unclassified_<em>Muribaculaceae</em>, and <em>Lachnospiraceae</em>_NK4A136_group, while inhibiting harmful bacterial populations such as <em>Faecalibaculum</em> and <em>Desulfovibrio</em>. These effects are related to the enhancement of the intestinal barrier, such as upregulation of Intectin expression, increased secretion of mucin MUC2, and increased expression of tight junction proteins. Spearman correlation analysis revealed significant associations between obesity related indicators, intestinal barrier, and gut microbiota, emphasizing the improvement effect of DBS-SDF on metabolic disorders through a healthy gut microenvironment. These findings indicate that DBS-SDF has enormous potential in the development of prebiotics or functional foods.</div></div>","PeriodicalId":12385,"journal":{"name":"Food Hydrocolloids for Health","volume":"7 ","pages":"Article 100217"},"PeriodicalIF":4.6,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144071354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Emulsion-enhanced synergy of walnut-derived hexapeptide (EPEVLR) and DHA in mitigating D-galactose-induced cognitive decline in mice","authors":"Like Lin ,&nbsp;Li Zhang ,&nbsp;Cong Li ,&nbsp;Lihua Jin ,&nbsp;Rui Wang ,&nbsp;Yu Shu ,&nbsp;Bang Chen ,&nbsp;Yehua Shen","doi":"10.1016/j.fhfh.2025.100215","DOIUrl":"10.1016/j.fhfh.2025.100215","url":null,"abstract":"<div><div>This study explored the synergistic effects of walnut-derived peptide (Glu-Pro-Glu-Val-Leu-Arg, EPEVLR) and docosahexaenoic acid (DHA) on ameliorating D-galactose (D-gal) induced cognitive dysfunction in mice. EPEVLR and DHA were integrated using ultrasonic mixing at a 2:1 molar ratio, resulting in a stable water-in-oil (W/O) emulsion labeled DHA@EP. DHA@EP was more effective than DHA or EPEVLR alone in alleviating D-gal-induced mice cognitive deficits. The underlying mechanisms included reducing oxidative stress, mitigating neuroinflammation, and stabilizing the cholinergic system. Structural characterization and theoretical calculations confirmed that EPEVLR and DHA formed a stable emulsion with droplet diameters below 10 μm, driven by hydrogen bonding between the nitrogen (N) and oxygen (O) atoms of EPEVLR and the hydrogen (H) atoms of DHA. <em>In vitro</em> digestion simulation experiments indicated that the W/O emulsion structure of DHA@EP significantly enhanced the intestinal stability of EPEVLR, likely due to the protective effect of the oil phase against enzymatic degradation in the gastrointestinal tract. These findings highlight the potential of DHA@EP as a functional food ingredient for cognitive improvement and provide insights into the synergistic use of bioactive peptides and fatty acids.</div></div>","PeriodicalId":12385,"journal":{"name":"Food Hydrocolloids for Health","volume":"7 ","pages":"Article 100215"},"PeriodicalIF":4.6,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143852264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and characterization of edible film containing keratin nanocarriers loaded with N. nucifera seedpod extract","authors":"Hina Fayaz Bhat ,&nbsp;Zarka Nasir ,&nbsp;Sheza Farooq ,&nbsp;Zuhaib F. Bhat ,&nbsp;Syed Mudasir Andrabi ,&nbsp;Abderrahmane Aït-Kaddour","doi":"10.1016/j.fhfh.2025.100216","DOIUrl":"10.1016/j.fhfh.2025.100216","url":null,"abstract":"<div><div>Edible films prepared from organic macromolecules have evolved as sustainable and biodegradable alternatives to standard petroleum-based packaging. The present study aimed to develop a novel carrageenan-based bioactive film using keratin nanocarriers loaded with <em>N. nucifera</em> seedpod extract (KNP-Ex). An ultrasonication-based method was used to prepare the nanocarriers using the keratin derived from goat hairs and the extract from <em>N. nucifera</em> seedpod extract, a waste-to-wealth concept. The films containing keratin nanocarriers (KNP) and extract-loaded keratin nanocarriers (KNP-Ex) were compared with the control films without any nanocarriers. The films were characterized for various physicochemical (such as SDS-PAGE, LC/MS, SEM, TEM, FTIR, and TGA analysis), physicomechanical (thickness, tensile strength, surface analysis, biodegradability, transparency, and solubility) and bioactive properties [antioxidant (DPPH-radical scavenging assay) and antimicrobial (disk diffusion assay)]. The results of our study indicate the potential of keratin nanocarriers as payloads to infuse bioactive properties and modify the mechanical and functional characteristics of the film. This film can provide fundamental protective functions and can be applied to improve the storage quality of foods.</div></div>","PeriodicalId":12385,"journal":{"name":"Food Hydrocolloids for Health","volume":"7 ","pages":"Article 100216"},"PeriodicalIF":4.6,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143859896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of microencapsulation on E. coli transcriptome under simulated gastric stress: Implications for oral fecal microbiota transplant delivery","authors":"Jiayi Li ,&nbsp;Yan Wang ,&nbsp;Han Yu ,&nbsp;Qinlin Zeng ,&nbsp;Peijia Ye ,&nbsp;Harold Corke ,&nbsp;Arakkaveettil Farha Kabeer ,&nbsp;Olivier Habimana","doi":"10.1016/j.fhfh.2025.100214","DOIUrl":"10.1016/j.fhfh.2025.100214","url":null,"abstract":"<div><div>Fecal microbiota transplantation (FMT) constitutes a practical therapeutic approach for a range of gastrointestinal disorders; nonetheless, its predominant delivery method, colonoscopy, limits its broader application adoption. Oral FMT offers a less invasive alternative, but the harsh gastric environment necessitates a protective delivery system. This research investigated how well microencapsulation with chitosan and chitosan-genipin safeguards <em>Escherichia coli</em> in conditions that simulate gastric transit. Microfluidic encapsulation generated uniform microcapsules, and simulated gastrointestinal analysis revealed superior stability for chitosan-genipin over chitosan alone. RNA sequencing and bioinformatics showed that chitosan-genipin significantly reduced simulated gastric fluid-induced transcriptomic disturbances in <em>E. coli</em>, lowering differentially expressed genes compared to chitosan or non-encapsulated samples controls. These findings suggest chitosan-genipin microencapsulation is a promising method for non-invasive and effective oral fecal microbiota transplant delivery.</div></div>","PeriodicalId":12385,"journal":{"name":"Food Hydrocolloids for Health","volume":"7 ","pages":"Article 100214"},"PeriodicalIF":4.6,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143847628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}