Food Hydrocolloids for Health最新文献

{"title":"Pea protein and p-coumaric acid conjugates: New evidence on in vitro digestion, modulation of gut microbiota, and anti-inflammatory activity","authors":"Xuxiao Gong ,&nbsp;Jie Lin ,&nbsp;Gwoncheol Park ,&nbsp;Saurabh Kadyan ,&nbsp;Quancai Sun ,&nbsp;Ravinder Nagpal ,&nbsp;Leqi Cui","doi":"10.1016/j.fhfh.2025.100245","DOIUrl":"10.1016/j.fhfh.2025.100245","url":null,"abstract":"<div><div>This study aimed to investigate the gut health benefits and anti-inflammatory property of pea protein isolates (PPI) as affected by conjugating with p-coumaric acid (p-CA). ¹³C NMR analysis confirmed the newly formed covalent bonds between PPI and p-CA occurred at the C8 position of p-CA. Results showed that after in vitro digestion, the conjugates exhibited higher protein digestibility compared to PPI. Dialysis was conducted to simulate small intestinal absorption and obtain non-absorbable fraction, which were utilized for human fecal fermentation and for the evaluation of anti-inflammatory activity in RAW 264.7 cells. Gut microbiota analysis revealed that PPI reduced the abundance of Bifidobacterium and increased Lactobacillus. However, after conjugation with p-CA, the inhibitory effect of PPI on Bifidobacterium was diminished, while its promotive effect on Lactobacillus was further strengthened. Meanwhile, PPI exhibited anti-inflammatory potential, as evidenced by the significant suppression of nitric oxide (NO) production and IL-6 expression. While the conjugates induced higher NO production compared to PPI, the levels remained lower than those induced by LPS alone, suggesting a partial reduction in anti-inflammatory potential after conjugation. Overall, this study provides new evidence that conjugation with phenolics can enhance pea protein's potential to positively influence gut microbiota composition, albeit with a reduced anti-inflammatory potential.</div></div>","PeriodicalId":12385,"journal":{"name":"Food Hydrocolloids for Health","volume":"8 ","pages":"Article 100245"},"PeriodicalIF":5.1,"publicationDate":"2025-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145216880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Formulation design of quercetin-loaded polyvinyl alcohol nanofibres for ocular drug delivery","authors":"Rabiah Bashir ,&nbsp;Adil Gani ,&nbsp;Asima Shah ,&nbsp;Hasham Shafi ,&nbsp;Abdul Aala Fazili ,&nbsp;Tabasum Ali ,&nbsp;Syed Naiem Raza ,&nbsp;Shabnam Kawoosa ,&nbsp;Nisar Ahmad Khan","doi":"10.1016/j.fhfh.2025.100244","DOIUrl":"10.1016/j.fhfh.2025.100244","url":null,"abstract":"<div><div>In the realm of ocular drug delivery, the limited bioavailability of pharmaceuticals presents a significant challenge. The development of alternative drug delivery systems aimed at enhancing the availability of medications at targeted sites is of considerable importance in addressing this issue. The research work aimed to design, formulate, and optimize a fast-dissolving nanofiber of quercetin and polyvinyl alcohol for an ocular delivery system. Response Surface Methodology (Box-Behnken design) was used for the optimisation of electrospinning parameters, including polymer concentration, flow rate, and voltage, resulting in enhanced drug encapsulation and the formation of smooth and uniform nanofibers. Morphological characterization was conducted using scanning electron microscopy, which demonstrated the development of smooth, uniform, bead-like porous fibers with the drug incorporated within the fibers in nanoform during the electrospinning process, rather than being deposited on the surface. The drug-excipient interaction was evaluated by FTIR analysis, which indicated compatibility between PVA and quercetin, with all the peaks of the quercetin retained in the optimized formulation. The conversion of quercetin from its crystalline form to an amorphous state was demonstrated through X-ray diffraction (XRD) and differential scanning calorimetry (DSC) analyses. The XRD pattern illustrated a reduction in the crystalline peaks characteristic of quercetin, while the DSC results indicated a decrease in the melting point of quercetin in nanofibers. The physical, chemical, and pharmaceutical characteristics of the optimized nanofibre formulation were appropriate and within limits. Disintegration was accomplished within 45 s, with approximately 100% of the active ingredient released within 10 min during an in vitro dissolution test. In contrast, the conventional eye drop formulation exhibited a release of only 50%. Eye irritation study results indicated that there was no visual sign of irritation in all experimental rabbits. The optimized formulation underwent an accelerated stability study and retained its original properties with negligible changes. There was no significant alteration in physical appearance, flexibility, disintegration time, or drug encapsulation, confirming its stability.</div></div>","PeriodicalId":12385,"journal":{"name":"Food Hydrocolloids for Health","volume":"8 ","pages":"Article 100244"},"PeriodicalIF":5.1,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145216285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Smart-responsive chitosan dressings: From microenvironmental sensing to multifunctional precision wound healing","authors":"Minghui Sun ,&nbsp;Yibo Zhang ,&nbsp;Mingwei Zhou","doi":"10.1016/j.fhfh.2025.100243","DOIUrl":"10.1016/j.fhfh.2025.100243","url":null,"abstract":"<div><div>Wound healing constitutes a multifaceted and dynamically regulated process that is modulated by a variety of microenvironmental factors such as pH, enzymes, and reactive oxygen species (ROS). Conventional wound dressings frequently fail to deliver the precise interventions necessary for the effective management of complex wounds. Chitosan, characterized by its high biocompatibility, biodegradability, and structural adaptability, exhibits enhanced self-healing photoactivity, rendering it an ideal candidate for the development of smart, responsive wound dressings. This review systematically examines the design strategies, molecular mechanisms, and therapeutic applications of chitosan-based smart dressings. It emphasizes their roles in infection control, inflammation reduction, angiogenesis, and tissue regeneration. Furthermore, we underscore the importance of multimodal systems incorporating adaptive feedback mechanisms (pH/ROS combinations, pH/enzyme and ROS/photothermal coupling) and explore their clinical potential in the treatment of diabetic foot ulcers, pressure ulcers, and other complex skin injuries. These findings provide a detailed framework for the development of the next generation of bio-responsive materials, fostering the integration of materials science with personalized wound care strategies.</div></div>","PeriodicalId":12385,"journal":{"name":"Food Hydrocolloids for Health","volume":"8 ","pages":"Article 100243"},"PeriodicalIF":5.1,"publicationDate":"2025-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145117853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of nanoemulsion-based sage seed gum coating containing cinnamon essential oil on shelf life extension of strawberry","authors":"Parastoo Asadi Shizari ,&nbsp;Fatemeh Davoodi ,&nbsp;Dayana Hassani ,&nbsp;Shahriyar Amiri ,&nbsp;Bahram Hassani","doi":"10.1016/j.fhfh.2025.100242","DOIUrl":"10.1016/j.fhfh.2025.100242","url":null,"abstract":"<div><div>In this study, the antimicrobial efficacy of a nanoemulsion (NE) based on sage seed gum (WSG) containing cinnamon essential oil (CEO) was evaluated as an edible coating to extend the shelf life of strawberries. CEO at a concentration of 400 ppm was used for NE preparation. The particle size, polydispersity index (PDI), zeta potential, and encapsulation efficiency of the NE were reported to be 148.5 nm, 0.27, –27.12 mV, and 86 %, respectively. Strawberries were coated with distilled water (control), CEO (400 ppm), WSG coating, and CEO-loaded NE coating, then stored at room temperature for 9 days. Evaluations were conducted on days 0, 3, 6, and 9 to assess mass loss, total soluble solids, total mesophilic bacteria count, total mold and yeast count, pH, titratable acidity, vitamin C content, firmness, and color parameters. The results showed that over time, mass loss, total soluble solids, total mesophilic bacteria, and total mold and yeast counts increased significantly (<em>p</em> &lt; 0.05), with the highest values observed in the control sample and the lowest in the NE -coated samples. Conversely, pH, titratable acidity, vitamin C content, and firmness decreased significantly (<em>p</em> &lt; 0.05) over time. The lowest total phenolic content was observed in the control group, while the highest was recorded in the NE -coated strawberries at the end of the storage period. Overall, the NE coating showed the most significant preservative effect among all treatments, followed by the conventional WSG coating. These findings highlight the potential of CEO NE as an effective edible coating to extend the shelf life of strawberries.</div></div>","PeriodicalId":12385,"journal":{"name":"Food Hydrocolloids for Health","volume":"8 ","pages":"Article 100242"},"PeriodicalIF":5.1,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145154511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of yeast cell-wall derived from a potential probiotic yeast isolate as a controlled release agent in a triple-layer microcapsule with potent anti S. aureus activity","authors":"Sima Shams Shargh ,&nbsp;Alireza Sadeghi ,&nbsp;Mahmoud Shams Shargh ,&nbsp;Fahimeh Hajinia ,&nbsp;Ali Moayedi","doi":"10.1016/j.fhfh.2025.100241","DOIUrl":"10.1016/j.fhfh.2025.100241","url":null,"abstract":"<div><div>Application of postbiotics derived from characterized probiotics (Probio) as efficient controlled release agents of the entrapped bioactives is a promising progress in microencapsulation strategies. In the present study, phylogenetic evolutionary analysis led to the identification of <em>Pichia kudriavzevii</em> as the potential Probio yeast isolated from fermented sprouted soybeans with 82.70 % survivability under simulated gastrointestinal (SGI) conditions and potent <em>in vitro</em> inhibitory activity against <em>Staphylococcus aureus</em> (88.21 %), which was significantly (<em>p</em> &lt; 0.05) higher than those of the other foodborne bacteria tested. The yeast isolate also showed antifungal, antioxidant and suitable adhesion capabilities. Moreover, anti-ochratoxigenic activity of viable and heat-killed cells of the isolate was approved according to the HPLC-based analysis. <em>In vivo</em> safety of the isolate was also confirmed in accordance with the blood biochemistry parameters and liver enzymes activity of the fed-chicken compared to the control group. <em>In vivo</em> inhibitory activity of viable and heat-killed cells of the isolate against <em>S. aureus</em> was equal to 74.39 % and 89.71 %, respectively. Cross-linking of the yeast cell-wall derived from the isolate with the alginate-chitosan construct was also approved through FTIR and SEM analyses. In addition, antibacterial profile of the construct under SGI conditions confirmed targeted delivery and controlled release of the entrapped chitosan.</div></div>","PeriodicalId":12385,"journal":{"name":"Food Hydrocolloids for Health","volume":"8 ","pages":"Article 100241"},"PeriodicalIF":5.1,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145044375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preserving and hydrogel-matrixing the bioactive properties of aromatic medicinal halophytic herbs from the coastline of the Iberian Peninsula","authors":"Tiago Parracho ,&nbsp;Pedro F. Cruz ,&nbsp;Claúdia C. Peralta ,&nbsp;Cândida G. Silva ,&nbsp;Maria Jorge Campos ,&nbsp;Marta Neves ,&nbsp;Rachel Cordeiro ,&nbsp;Daniela Trindade ,&nbsp;Carla Moura ,&nbsp;Zaida L. Almeida ,&nbsp;Cidália D. Pereira ,&nbsp;Carla Guimarães ,&nbsp;Rui M.M. Brito ,&nbsp;Mauro Guerra ,&nbsp;Fernando Reboredo ,&nbsp;Paula Veríssimo ,&nbsp;Vânia Ribeiro ,&nbsp;Daniela C. Vaz","doi":"10.1016/j.fhfh.2025.100239","DOIUrl":"10.1016/j.fhfh.2025.100239","url":null,"abstract":"<div><div>Edible wild plants are part of the ethnobotanic heritage of a certain geographical area and are important sources of essential oils, antioxidants, minerals, and special flavours. <em>Corema album</em> (Portuguese crowberry), <em>Crithmum maritimum</em> (sea fennel), <em>Eryngium maritimum</em> (sea holly), <em>Helichrysum italicum</em> (curry plant) and <em>Otanthus maritimus</em> (cottonweed) wildly flourish along the sandy dunes of the coast of the Iberian Peninsula. These plants are locally known for their beneficial properties, with important value for food, cosmetics and/or medicinal applications. Hence, leaves of these endemic species were collected at four different locations and submitted to different preserving treatments (oven-drying, freezing, and freeze-drying). Acetonic extracts of the different plants submitted to the different post-harvesting treatments were analysed regarding their antioxidant capacities and phenolic contents. Plant extracts were also analysed by diffusion-ordered nuclear magnetic resonance spectroscopy (DOSY-NMR). In general, freeze-drying was the best method of preserving plant minerals, antioxidants (∼4 mgVCEAC/g fw) and polyphenols (∼5 mgGAE/g fw). Minerals were quantified via energy-dispersive X-ray fluorescence spectrometry, and despite their location, all plants were rich in Ca, Cl, K, S and P. Hierarchical clustering and principal component analyses (PCA) pointed towards chemical/metabolic proximity between taxonomic families. Alginate hydrogels loaded with 0.1 % and 0.2 % (w/v) of extracts presented homogenous surface properties by scanning electron microscopy, good mechanical tensile strength (∼30 MPa) and antibacterial activity against <em>S. aureus</em>. Edible alginate hydrogels enriched with plant extracts hold great nutraceutical potential to be used as natural preservatives for food coating and packaging or as sources of bioactive compounds for biomedical applications.</div></div>","PeriodicalId":12385,"journal":{"name":"Food Hydrocolloids for Health","volume":"8 ","pages":"Article 100239"},"PeriodicalIF":5.1,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145026979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent advances in plant protein microgels encapsulated with bioactive compounds","authors":"Srutee Rout ,&nbsp;Sena Bakir ,&nbsp;Sonali Khanal ,&nbsp;Gurjeet Kaur ,&nbsp;Zakir Showkat Khan ,&nbsp;Thameed Aijaz ,&nbsp;Jyoti Sharma ,&nbsp;Mohmad Sayeed Bhat ,&nbsp;Farhana Mehraj ,&nbsp;Ghazaleh Sadeghi Vahid ,&nbsp;Dinesh Kumar ,&nbsp;Prem Prakash Srivastav","doi":"10.1016/j.fhfh.2025.100238","DOIUrl":"10.1016/j.fhfh.2025.100238","url":null,"abstract":"<div><div>Protein microgels and other novel food structuring methods have garnered attention to increase plant protein functionality. Microgels, small particles (0.5–5 μm) that trap a lot of solvent, are made of a three-dimensional network of cross-linked biopolymer molecules. Protein microgels exhibit structural adaptability that makes them excellent carriers for lipophilic bioactives, protecting them from degradation and instability in aqueous systems. Additionally, their tunable design allows controlled release, enhancing bioavailability and functional efficacy in food and nutraceutical applications. The integration of scientific advances with food technology positions plant protein-based microgels as transformative tools in developing sustainable, protein-rich, and functional plant-based food systems. These advances in protein microgels prompt this review of growing plant protein sources, microgel formation, stabilization technologies, encapsulation with bioactive compounds, bioactive component transport, focusing on their functional properties, structural features, and applications. Knowing these trends can help us understand how sustainable, functioning food systems will emerge and solve global environmental and nutritional challenges.</div></div>","PeriodicalId":12385,"journal":{"name":"Food Hydrocolloids for Health","volume":"8 ","pages":"Article 100238"},"PeriodicalIF":5.1,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145044376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Green synthesis of biocompatible silver nanoparticles from Persicaria hydropiper leaves: Therapeutic and catalytic applications","authors":"Shajahan Talukder ,&nbsp;Md. Shahin Ali ,&nbsp;Sangita Kundu ,&nbsp;Md. Abdul Aziz ,&nbsp;Md. Rezaul Karim ,&nbsp;Md. Habibur Rahman ,&nbsp;M. Ahasanur Rabbi ,&nbsp;Md. Rowshanul Habib","doi":"10.1016/j.fhfh.2025.100237","DOIUrl":"10.1016/j.fhfh.2025.100237","url":null,"abstract":"<div><div>The study presents a sustainable method for synthesizing biocompatible silver nanoparticles from <em>Persicaria hydropiper</em> leaves (PH-AgNPs) with promising anti-inflammatory, antioxidant, anticancer, antimicrobial, and catalytic potentials. UV–vis analysis confirmed PH-AgNPs formation with a surface plasmon resonance (SPR) peak at 455 nm. FTIR spectra revealed the constituents of leaf extract involved in silver ion reduction. Characterization of PH-AgNPs was done using X-ray diffraction, dynamic light scattering, scanning electron microscopy, and energy dispersive X-ray techniques. This characterization confirmed that PH-AgNPs exhibited a crystalline, spherical shape with a strong silver peak. PH-AgNPs showed blood compatibility and a non-toxic nature as compared with Triton-X. PH-AgNPs exhibited moderate anti-inflammatory and antioxidant activities compared to diclofenac sodium and ascorbic acid. PH-AgNPs also showed potential as catalysts for the degradation of harmful dyes. In the anticancer experiment, PH-AgNPs treatments significantly (<em>p</em> &lt; 0.05) decreased viable EAC (Ehrlich Ascites Carcinoma) cells and lowered weight gain in mice. PH-AgNPs (<em>p</em> &lt; 0.05) restored blood counts and improved liver structure in treated mice <em>versus</em> untreated controls. Proapoptotic genes encoding p53 and Bax proteins were overexpressed in treated EAC cells, confirming the apoptotic hallmarks seen in fluorescence microscope images of these treated cells. PH-AgNPs demonstrated potent antibacterial activity, particularly against Shigella boydii, while erythromycin was the most effective against the same bacterium. PH-AgNPs combined with tetracycline showed the highest synergistic effect (37.26 %) against <em>Escherichia coli</em>. PH-AgNPs showed the lowest minimum inhibitory concentration (9.38 mg/mL) against Bacillus cereus. Therefore, the green-fabricated PH-AgNPs hold significant biological and environmental importance, offering potential for therapeutic and environmentally friendly applications.</div></div>","PeriodicalId":12385,"journal":{"name":"Food Hydrocolloids for Health","volume":"8 ","pages":"Article 100237"},"PeriodicalIF":5.1,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145019178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Structural characterization and bioactivity of sulfated Galactan GSSG-2 from Gracilaria salicornia: Antioxidant and anticancer potential","authors":"Mythileeswari Lakshmikanthan ,&nbsp;Sakthivel Muthu ,&nbsp;Peer Mohamed Deen Mohamed ,&nbsp;Kathiravan Krishnan ,&nbsp;Nagaraj Karuppiah ,&nbsp;Ramamoorthy Karuppan ,&nbsp;Shenbhagaraman Ramalingam ,&nbsp;Anandhi Subramanian ,&nbsp;Bharathi Venkatachalam ,&nbsp;Gholamreza Abdi","doi":"10.1016/j.fhfh.2025.100236","DOIUrl":"10.1016/j.fhfh.2025.100236","url":null,"abstract":"<div><div>The sulfated galactan GSSG-2, isolated from <em>Gracilaria salicornia</em>, was investigated for its antioxidant and anticancer properties. Polysaccharides derived from marine sources are well-known for their distinctive bioactivities, making them promising candidates for pharmaceutical applications. Purification of GSSG-2 was achieved through a two-step procedure consisting of anion-exchange chromatography utilizing a DEAE-Sepharose column, followed by size-based separation via Sephadex G-100 gel filtration chromatography. Its structural features were characterized using elemental analysis, high-performance gel permeation chromatography (HPGPC), high-performance liquid chromatography (HPLC), Fourier-transform infrared spectroscopy (FT-IR), and nuclear magnetic resonance (NMR) spectroscopy. The antioxidant capacity of GSSG-2 was measured at concentrations from 25 to 150 µg/mL using assays such as DPPH radical scavenging, inhibition of superoxide anions, ferric reducing antioxidant power (FRAP), and total reducing power. In all assays, l-ascorbic acid served as the reference standard. Cytotoxic effects and induction of apoptosis in A549 human lung carcinoma cells were assessed employing MTT assays and acridine orange/ethidium bromide (AO/EtBr) fluorescence staining. GSSG-2 demonstrated a molecular weight of 70.61 kDa, with galactose identified as the dominant monosaccharide unit (328.92 mg/g). Its antioxidant activity showed a dose-dependent increase, ranging from 19.84% to 88.59%. The IC₅₀ value determined by the MTT assay was 100 µg/mL, and cell viability dropped dramatically to 16.0% at 250 µg/mL. Results from AO/EtBr staining further corroborated the time-dependent enhancement of apoptotic cell death. The strong antioxidant and anticancer effects of GSSG-2 are attributed to its structural components, notably the esterified sulfate moieties and β-(1→3)-linked d-galactopyranose units. These results underscore the potential of this compound as a therapeutic agent targeting oxidative stress and cancer.</div></div>","PeriodicalId":12385,"journal":{"name":"Food Hydrocolloids for Health","volume":"8 ","pages":"Article 100236"},"PeriodicalIF":5.1,"publicationDate":"2025-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144827474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extraction, purification, structure characterization of polysaccharides from Cistanche deserticola and their biological effects","authors":"Faqin Tao ,&nbsp;Baotang Zhao ,&nbsp;Shen Song ,&nbsp;Yunfei Xu ,&nbsp;Ji Zhang","doi":"10.1016/j.fhfh.2025.100235","DOIUrl":"10.1016/j.fhfh.2025.100235","url":null,"abstract":"<div><div>The polysaccharide fraction (CDP-D2-N1) from the stem of Cristanche deseticola was obtained after extraction and purification. The structural characteristics and in vitro antioxidant and anti-inflammatory activities of CDP-D2-N1 were investigated by physical, chemical and instrumental analyses. The results showed that CDP-D2-N1 (127.784 kDa) is a heterogeneous polysaccharide with an irregular non-crystalline structure, containing mainly Rha:Ara:Xyl:Gal:Gal:GlcA:Glc:GalA, and a molar ratio of 6.75 : 26.68 : 1.56 : 37.87 : 3.94 : 2.17 : 21.02. Combined with the results of NMR spectroscopy and methylation analyses, the main chain of CDP-D2-N1 was determined to be →4)-α-<span>d</span>-GalpA-(1→,→3,6)-β-<span>d</span>-Galp-(1→, →5)-α-<span>l</span>-Araf-(1→, containing a small amount of fragment →3,4)-β-<span>d</span>-Galp-(1→ and →3)-β-<span>d</span>-Galp-(1→, and the CDP-D2-N1 structure was inferred. In vitro assays demonstrated that CDP-D2-N1 possessed significant antioxidant capacity and attenuated inflammation induced by lipopolysaccharide (LPS) from <em>Escherichia coli</em> O55:B5 in bovine endothelial cells (bEEcs) by inhibiting the expression levels of TNF-α, IL-6, TLR 4 and IL-1.</div></div>","PeriodicalId":12385,"journal":{"name":"Food Hydrocolloids for Health","volume":"8 ","pages":"Article 100235"},"PeriodicalIF":5.1,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144780240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}