Food Additives and Contaminants Part A-chemistry Analysis Control Exposure & Risk Assessment最新文献

{"title":"Determination of oxyphenisatine and its total ester derivatives content in fermented green plum by ultra performance liquid chromatography-tandem mass spectrometry.","authors":"Zhihua Zhang, Zhanqiang Hu, Baolin Xia","doi":"10.1080/19440049.2024.2446709","DOIUrl":"10.1080/19440049.2024.2446709","url":null,"abstract":"<p><p>Illegal additives such as oxyphenisatine and its esters are prevalent in the slimming food industry, necessitating a robust analytical method for their detection. This study presents a novel UPLC-MS/MS method for the rapid and accurate quantification of total oxyphenisatine levels in fermented green plum, following hydrolysis of its esters. An efficient ultrasonic extraction with a methanol and 0.1 mol/L NaOH mixture (5:5, <i>v/v</i>) was optimised to hydrolyse esters to oxyphenisatine within 18 min. Chromatographic separation was conducted on a C18 column (Waters Acquity UPLC BEH, 2.1 × 100 mm, 1.7 μm) with a mobile phase of 5 mmol/L ammonium acetate and acetonitrile under gradient elution at a flow rate of 0.3 mL/min. The method demonstrated linearity (<i>r</i>² > 0.999) over 0.1-500 µg/L, with a LOD of 10 µg/kg and LOQ of 30 µg/kg. Quantitative analysis employed positive ion multi-response monitoring and external standardisation, achieving recoveries of 92.4-97.0% and RSDs of 2.9-4.1%. Application to ten real samples gave a 90% detection rate, with measured values closely aligning with theoretical predictions (-11.3 to 13.2% relative difference) and oxyphenisatine content ranging from 159 µg/kg to 452 mg/kg. This UPLC-MS/MS method provides a reliable and efficient tool for monitoring the presence of oxyphenisatine and its derivatives in the context of food safety.</p>","PeriodicalId":12295,"journal":{"name":"Food Additives and Contaminants Part A-chemistry Analysis Control Exposure & Risk Assessment","volume":" ","pages":"169-179"},"PeriodicalIF":2.3,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142947158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tissue histology and depuration of per- and polyfluoroalkyl substances (PFAS) from dairy cattle with lifetime exposures to PFAS-contaminated drinking water and feed.","authors":"Sara J Lupton, David J Smith, Erin B Howey, Ann S Predgen, Carrie E Schmidt, Eric Scholljegerdes, Shanna Ivey, Emilio Esteban, John J Johnston","doi":"10.1080/19440049.2024.2444560","DOIUrl":"10.1080/19440049.2024.2444560","url":null,"abstract":"<p><p>Plasma, milk and tissue samples were collected from 30 dairy cattle (0.4 to 8.9 years of age) with lifetime exposures to perfluoroalkyl substances (PFAS) removed from a PFAS-contaminated farm and provided PFAS-free feed and water. Twenty cattle were slaughtered 2 weeks after removal from the farm and tissues were collected for histological and residue analyses. Milk and/or plasma were collected from all remaining cattle at 2-week intervals and milk samples were collected daily but were analyzed at the same intervals as plasma samples. The remaining cattle were slaughtered 20 and 22 weeks after the initial set of 20 animals were slaughtered. While many incidental and normal background findings were noted on histological evaluation, no consistent histological finding was associated with PFAS exposure. Perfluoroalkyl carboxylic acids (PFCA) and perfluoro butane sulfonic acid (PFBS) were not generally detected in milk, plasma and tissues, but perfluoroalkyl sulfonic acids (PFSA) were quantifiable throughout the 22-week withdrawal period in most matrices. Estimated plasma half-lives of perfluorohexane sulfonic acid (PFHxS), perfluoroheptane sulfonic acid (PFHpS), linear perfluorooctane sulfonic acid (L-PFOS), perfluoro-3-methyl heptanesulfonate (3Me-PFOS) and perfluoro-6-methyl heptanesulfonate (6Me-PFOS) ranged from 4 to 10 weeks, but the estimates were associated with large confidence intervals. Across animal status (heifer, lactating, dry), natural log transformed (Ln) plasma residues of PFHxS and L-PFOS were generally well correlated with Ln-transformed PFHxS and L-PFOS residues in lung, muscle, liver and kidney (R², 0.7572 to 0.9394) whereas the strongest relationships of Ln-transformed L-PFOS residues among tissues were between lung and liver, kidney and muscle (R², 0.8287 to 0.9138).</p>","PeriodicalId":12295,"journal":{"name":"Food Additives and Contaminants Part A-chemistry Analysis Control Exposure & Risk Assessment","volume":" ","pages":"223-239"},"PeriodicalIF":2.3,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142947088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a direct competitive enzyme-linked immunosorbent assay (dc-ELISA) for the detection of scopolamine in wheat.","authors":"Gurmit Singh, Ligia Velasquez, Terence Koerner, Anne-Catherine Huett, Nathalie Gillard","doi":"10.1080/19440049.2024.2435335","DOIUrl":"10.1080/19440049.2024.2435335","url":null,"abstract":"<p><p>A competitive direct enzyme-linked immunosorbent assay (dc-ELISA) was developed for the detection and quantification of scopolamine (SCO) in wheat flours and cereal samples (multigrain, oat and barley). The limit of quantification (IC₂₀) of the established method was 6.00 ± 1.20 ng/g, with the limit of detection (IC₁₀) being 2.4 ± 0.6 ng/g in wheat flour with a coefficient of variation (CV) less than 20%. The assay was highly specific to SCO and nor-scopolamine, with no cross-reactivity to other similar structures. In spiked wheat flours the recoveries ranged from 84% to 104% with CVs of less than 20% and the recovery from a Food Analysis Performance Assessment Scheme (FAPAS) buckwheat control sample was 118%. A comparison of spiked wheat flour and a FAPAS control sample showed similar results to those determined by classical LC-MS/MS methods. A small retail survey of wheat flours and cereal samples was conducted using this ELISA method and a LC-MS/MS method, which showed scopolamine was not detected in any of these survey samples by either method. This method is suitable for rapid quantitation of SCO in wheat flours and cereal samples.</p>","PeriodicalId":12295,"journal":{"name":"Food Additives and Contaminants Part A-chemistry Analysis Control Exposure & Risk Assessment","volume":" ","pages":"240-248"},"PeriodicalIF":2.3,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142789448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of glyphosate, glufosinate, and their metabolites in multi-floral honey for food safety.","authors":"Giulia Rampazzo, Maria Nobile, Stefania Carpino, Luca Chiesa, Sergio Ghidini, Teresa Gazzotti, Sara Panseri","doi":"10.1080/19440049.2024.2441752","DOIUrl":"10.1080/19440049.2024.2441752","url":null,"abstract":"<p><p>Beehives can accumulate environmental contaminants as bees gather pollen, propolis, and water from their surroundings, contaminating hive products like honey. Moreover, in multifloral environments, bees can interact with plants treated with different pesticides, often causing higher pesticides concentrations in multi-floral honey than in mono-floral varieties. Glyphosate and glufosinate are both widely used herbicides. Glyphosate accounted for one-third of herbicide sales in Europe in 2017 and continues to raise health concerns, including its potential carcinogenicity. While the European Commission extended glyphosate's authorisation for another 10 years in 2023, concerns remain about its impact on biodiversity and human health. This study aimed to monitor the presence of glyphosate, glufosinate, and their metabolites in 100 samples of multifloral honey representing Italian production by analysis using IC-HRMS. Results indicated that 12% of honey samples contained glyphosate residues ranging from > LOQ to 45 ng g^-1, with the highest concentrations detected in the Puglia region. No sample exceeded the maximum residue levels set by EU regulations. Glufosinate and its metabolites were not detected in any samples. These findings underscore the need for continued monitoring of pesticide residues in honey, particularly given the potential 'cocktail effect' of multiple contaminants and their combined toxicity. This study highlights the importance of safeguarding consumer health, especially in vulnerable populations, by addressing gaps in data on pesticide residue levels.</p>","PeriodicalId":12295,"journal":{"name":"Food Additives and Contaminants Part A-chemistry Analysis Control Exposure & Risk Assessment","volume":" ","pages":"213-222"},"PeriodicalIF":2.3,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142961986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation and identification of two novel PDE-5 inhibitors illegally added to pressed candies.","authors":"Li-Hong Zhao, Xiao-Pu Liu, Pei-Zhi Dong, Xin-Hua Xiang, Guo-Hua Shen","doi":"10.1080/19440049.2024.2445201","DOIUrl":"10.1080/19440049.2024.2445201","url":null,"abstract":"<p><p>Two novel phosphodiesterase 5 (PDE-5) inhibitors were detected in pressed candy using high-performance liquid chromatography (HPLC)-diode array detection. Following extraction with acetonitrile and sonication, the compounds were isolated and purified <i>via</i> semi-preparative liquid chromatography. Structural characterisation was achieved through high-resolution mass spectrometry (HRMS) and nuclear magnetic resonance (NMR) spectroscopy. The results indicate that both compounds possess identical UV absorption spectra. The molecular formulas of compounds 1 and 2 are C₂₃H₃₂N₄O₄ and C₂₂H₃₀N₄O₄, respectively, each featuring a pyrimidinone benzene structure with a single methylene group (-CH₂) variation. As studies on these illicit compounds in functional foods have not been conducted, regulatory agencies must take note and incorporate them for the assessment of illicit additives in functional and healthcare foods.</p>","PeriodicalId":12295,"journal":{"name":"Food Additives and Contaminants Part A-chemistry Analysis Control Exposure & Risk Assessment","volume":" ","pages":"180-190"},"PeriodicalIF":2.3,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142978111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative assessment of commercial ELISA kits for the screening of chloramphenicol residues in meat and aquaculture products according to European Regulation (EU) 2021/808 and to the new Reference Point for Action (Commission Regulation (EU) 2019/1871).","authors":"Lucille Rousseau, Romain Ménager, Céline Hédou, Eric Verdon, Christophe Soumet, Valérie Gaudin","doi":"10.1080/19440049.2024.2435326","DOIUrl":"10.1080/19440049.2024.2435326","url":null,"abstract":"<p><p>This study covered the evaluation of performance characteristics and validation of five commercial ELISA kits for the detection of a banned antimicrobial, chloramphenicol (CAP), in muscle and aquaculture products. CAP has been banned in the European Union since 1994, but is still authorised in some countries in the world. In 2019, the European Union set a new Reference Point for Action (RPA), decreasing the acceptable limit of CAP in animal tissues for human consumption from 0.30 µg/kg to 0.15 µg/kg. Validations were performed according to the European Regulation EC/2021/808 and to the European Guideline on Screening Method Validation (2023). The detection capabilities CCβ were all determined under the RPA, but were 3 to 15 times higher than the announced limit of detection (LOD). False negative rates were satisfactory for all the kits (≤ 5%) and false positive rates were acceptable. All of them were found out to be applicable to aquaculture products and meat at a common CCβ.</p>","PeriodicalId":12295,"journal":{"name":"Food Additives and Contaminants Part A-chemistry Analysis Control Exposure & Risk Assessment","volume":" ","pages":"191-202"},"PeriodicalIF":2.3,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142767655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Simultaneous determination of twelve microcystins in algal supplements on the Chinese market and possible risks to exposed populations.","authors":"Tongtong Liu, Shanshan Sun, Shaoming Jin, Xiao Ning, Po Chen, Hui Xiao, Jin Cao","doi":"10.1080/19440049.2024.2443933","DOIUrl":"10.1080/19440049.2024.2443933","url":null,"abstract":"<p><p>Algae supplements are widely recognized for their nutritional benefits and are commonly marketed as natural health products. However, concerns regarding contamination with cyanobacterial toxins have been raised. Moreover, there is very little data regarding the potential contamination of algal supplements on the Chinese market by these toxins. In this study, we employed a validated solid-phase extraction ultra-high-performance liquid chromatography-tandem mass spectrometry (SPE-UHPLC-MS/MS) method to analyze algal supplements available in China. Therefore, this study optimized the extraction conditions for twelve microcystins (MCs) simultaneously using response surface methodology (RSM) and analyzed <i>via</i> UHPLC-MS/MS. Method validation was conducted in four supplement matrices (capsule, liquid, powder, and tablet) to ensure the method's accuracy, sensitivity, and reproducibility. Analysis of nineteen commercial algae products available in China using the validated method revealed the presence of four MCs: microcystin-LF (MC-LF), microcystin-LR (MC-LR), microcystin-LA (MC-LA), and microcystin-YR (MC-YR). Furthermore, seven products were found to contain one or more of the MCs, with two products exceeding the 1 μg/g MC limit. These findings underscore the effectiveness of the validated method in assessing MC contamination of algae supplements and consequently identifying consumers expected to be at risk from prolonged exposure to recommended daily algal supplements.</p>","PeriodicalId":12295,"journal":{"name":"Food Additives and Contaminants Part A-chemistry Analysis Control Exposure & Risk Assessment","volume":" ","pages":"259-272"},"PeriodicalIF":2.3,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142947087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Residue depletion and withdrawal time estimation of tilmicosin in black-bone silky fowls after administration via drinking water.","authors":"Li Zhang, Lijuan Yuan, Mengjun Ye, Jianjun Xiang, Yifan Dong, Qiegen Liao, Suyan Qiu, Dawen Zhang, Xiren Yu","doi":"10.1080/19440049.2024.2445784","DOIUrl":"10.1080/19440049.2024.2445784","url":null,"abstract":"<p><p>In this study, residue depletion and withdrawal time estimation of tilmicosin were examined in Taihe black-bone silky fowls (TBSFs) after oral administration for three consecutive days at a dose of 75 mg/L in water. The tilmicosin concentrations in liver, kidney, muscle, and skin/fat of TBSFs collected from different time points (0.16, 1, 3, 5, 7, 9, 12, 20, 30, 40 days after last administration) were determined by UPLC-MS/MS. The results indicated that the tilmicosin concentrations in TBSFs tissues varied significantly, and kidney had the highest average concentrations (2604.65 ± 4625.20 μg/kg), followed by liver (1125.54 ± 1479.24 μg/kg), skin/fat (372.81 ± 428.33 μg/kg), and muscle (104.52 ± 143.95 μg/kg). Meanwhile, tilmicosin was still detected in all the four studied tissues (liver, kidney, skin/fat, and muscle) of TBSFs at the last time point (40th day after administration), suggesting that tilmicosin in TBSFs depleted slowly. Based on our experiments, the recommended withdrawal time of tilmicosin for TBSFs after oral administration for three consecutive days at a dose of 75 mg/L in water should be 32 days, which is much longer than the duration specified by Chinese regulatory authorities (10 days), and the abundance of melanin in TBSFs might be responsible for this phenomenon. Hence, a special use and withdrawal procedure of veterinary drugs in TBSFs is needed, and it is essential to focus on potential involvement of melanin in tilmicosin accumulation.</p>","PeriodicalId":12295,"journal":{"name":"Food Additives and Contaminants Part A-chemistry Analysis Control Exposure & Risk Assessment","volume":"42 2","pages":"203-212"},"PeriodicalIF":2.3,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143046113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characteristics of heavy metal migration from virgin PET and recycled PET plastic bottles in beverage products.","authors":"Ratih Dewi Sujana, Hanifah Nuryani Lioe, Nugraha Edhi Suyatma","doi":"10.1080/19440049.2024.2428745","DOIUrl":"10.1080/19440049.2024.2428745","url":null,"abstract":"<p><p>Polyethylene terephthalate (PET) is one of the most commonly used packaging materials for beverages. The increased use of PET has contributed to the rise in plastic waste. Recycled PET (R-PET) is a solution to address this environmental issue. This study aims to determine the migration level of Pb, Cd, Hg, and Sb in PET bottles, evaluate the effect of plastic types (virgin PET and R-PET) and testing conditions (according to BPOM following national regulation and EU following international regulation) on the migration from heavy metals into food. Sixty test samples of virgin PET and R-PET bottles, obtained from four packaging industries in Indonesia, were analyzed for Cd, Hg, Pb, and Sb concentrations using ICP-MS. The results showed that the highest concentrations of heavy metal migration for Cd and Pb were in a virgin PET sample according to BPOM, respectively, at 28 ± 2 ng/L and 636 ± 22 ng/L. Meanwhile, the highest concentrations of Hg and Sb were in a R-PET sample according to EU, respectively, at 469 ± 91 ng/L and 5042 ± 617 ng/L. There was a significant difference in heavy metal levels between the two types of plastic (virgin PET and R-PET) and two testing conditions (BPOM and EU). However, all the obtained concentrations were below the permitted limits of national and EU regulations.</p>","PeriodicalId":12295,"journal":{"name":"Food Additives and Contaminants Part A-chemistry Analysis Control Exposure & Risk Assessment","volume":" ","pages":"273-280"},"PeriodicalIF":2.3,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142828166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Glyphosate losses through various stages of coffee production and consequences for human exposure.","authors":"Gerhard Bytof, Oliver Suesse-Herrmann, Meike Holtmann, Jessica A Falenski, Viviane Theurillat, Gerhard Eisenbrand","doi":"10.1080/19440049.2024.2427667","DOIUrl":"10.1080/19440049.2024.2427667","url":null,"abstract":"<p><p>Green coffee beans, rejected for commercial use because of glyphosate contamination, were examined to monitor their glyphosate levels from harvest, through roasting, until various coffee extractions. The green beans, Arabica and Robusta, exhibited glyphosate levels above the EU-MRL (0.14-0.21 mg/kg), representing a worst-case scenario. The beans were roasted to different degrees and subsequently used for different coffee preparations. As a result of roasting (>200 °C), glyphosate contents were reduced, frequently by more than 73%. Remarkably, up to 9% of initial glyphosate was removed together with the silverskin, already at lower temperatures. Filtered and instant coffee beverages prepared from respective coffee samples resulted in virtually quantitative glyphosate transfer. Glyphosate transfer was significantly less for espresso, and ristretto, apparently due to the reduced amounts of water used for extraction. Aminomethylphosphonic acid (AMPA) was not detectable on any process level, confirming that AMPA is not a thermal glyphosate degradation product. In conclusion, compelling evidence is provided that glyphosate contamination becomes considerably reduced during roasting, whereas beverage preparation contributes at best to a minor further reduction. In consequence, even unusually high initial glyphosate loads in green beans are strongly reduced by the roasting process, resulting in a final cup content of <0.4 µg.</p>","PeriodicalId":12295,"journal":{"name":"Food Additives and Contaminants Part A-chemistry Analysis Control Exposure & Risk Assessment","volume":" ","pages":"59-74"},"PeriodicalIF":2.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142647262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}