Isolation and identification of two novel PDE-5 inhibitors illegally added to pressed candies.

IF 2.3 3区农林科学 Q2 CHEMISTRY, APPLIED

Food Additives and Contaminants Part A-chemistry Analysis Control Exposure & Risk Assessment Pub Date : 2025-01-13 DOI:10.1080/19440049.2024.2445201

Li-Hong Zhao, Xiao-Pu Liu, Pei-Zhi Dong, Xin-Hua Xiang, Guo-Hua Shen

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Abstract

Two novel phosphodiesterase 5 (PDE-5) inhibitors were detected in pressed candy using high-performance liquid chromatography (HPLC)-diode array detection. Following extraction with acetonitrile and sonication, the compounds were isolated and purified via semi-preparative liquid chromatography. Structural characterisation was achieved through high-resolution mass spectrometry (HRMS) and nuclear magnetic resonance (NMR) spectroscopy. The results indicate that both compounds possess identical UV absorption spectra. The molecular formulas of compounds 1 and 2 are C₂₃H₃₂N₄O₄ and C₂₂H₃₀N₄O₄, respectively, each featuring a pyrimidinone benzene structure with a single methylene group (-CH₂) variation. As studies on these illicit compounds in functional foods have not been conducted, regulatory agencies must take note and incorporate them for the assessment of illicit additives in functional and healthcare foods.

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压榨糖果中非法添加的两种新型PDE-5抑制剂的分离鉴定。

采用高效液相色谱-二极管阵列检测技术，在压榨糖果中检测到两种新型磷酸二酯酶5 （PDE-5）抑制剂。经乙腈提取和超声处理后，采用半制备液相色谱分离纯化化合物。结构表征是通过高分辨率质谱（HRMS）和核磁共振（NMR）光谱来实现的。结果表明，两种化合物具有相同的紫外吸收光谱。化合物1和2的分子式分别为C23H32N4O4和C22H30N4O4，均为一个亚甲基（-CH2）变异的嘧啶酮苯结构。由于尚未对功能食品中的这些非法化合物进行研究，监管机构必须注意并将其纳入评估功能食品和保健食品中的非法添加剂。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Food Additives and Contaminants Part A-chemistry Analysis Control Exposure & Risk Assessment CHEMISTRY, APPLIED-FOOD SCIENCE & TECHNOLOGY

CiteScore

7.40

自引率

6.90%

发文量

136

审稿时长

3 months

期刊介绍： Food Additives & Contaminants: Part A publishes original research papers and critical reviews covering analytical methodology, occurrence, persistence, safety evaluation, detoxification and regulatory control of natural and man-made additives and contaminants in the food and animal feed chain. Papers are published in the areas of food additives including flavourings, pesticide and veterinary drug residues, environmental contaminants, plant toxins, mycotoxins, marine biotoxins, trace elements, migration from food packaging, food process contaminants, adulteration, authenticity and allergenicity of foods. Papers are published on animal feed where residues and contaminants can give rise to food safety concerns. Contributions cover chemistry, biochemistry and bioavailability of these substances, factors affecting levels during production, processing, packaging and storage; the development of novel foods and processes; exposure and risk assessment.