F1000Research最新文献

{"title":"A protocol for the development of PhyCaRe: An extension of the CARE guideline for physiotherapy using the Delphi method.","authors":"Waqar M Naqvi, Gaurav Mishra, Aishwarya A Pashine, Sakshi P Arora, Sonia Gupta, Chanan Goyal, Ashish R Varma, Zahiruddin Quazi, Ramprasad Muthukrishnan, Praveen Kumar Kandakurti, Laxmikant Umate","doi":"10.12688/f1000research.138599.1","DOIUrl":"10.12688/f1000research.138599.1","url":null,"abstract":"<p><strong>Background: </strong>Background Case reports are one of the important forms of documentation and publication of clinical physiotherapy presenting the first line of evidence in scientific literature. In order to provide a systematic and precise structure for reporting and presenting cases, the CAse REport (CARE) guidelines were established in 2013. However, these guidelines present limitations as reporting requires items of specific specialties following the checklist. Authors from different specialities have developed CARE extensions specifying the characteristic features of corresponding fields, however, an extension dealing with physiotherapy assessment and line of management in the CARE guidelines is proposed as Physiotherapy CAse REport (PhyCARE).</p><p><strong>Method: </strong>A survey will be conducted using standard e-Delphi methodology using Google forms®. ¹ ^, ³ ^, ⁵ The e-Delphi questionnaire shall comprise responses on the relevance of each item of the CARE 2013 guideline with respect to physiotherapy. This shall be followed by suggestions from e-Delphi members regarding the incorporation of new items or adaptations of the existing items in the CARE 2013 statement using controlled feedback. Accordingly, based on the responses and suggestions from the e-Delphi experts, the core committee will draft the initial version of the PhyCARE checklist, which will be included in the second round of the e-Delphi process. Administration of the questionnaire as well as the sequential rounds shall continue unless a comprehensive set of findings with concurred definitions are obtained. Due to the fact that there is no requirement set for the number of e-Delphi rounds, the entire procedure will be carried out online. Subsequently, followed by pilot testing, submission of the CARE extension for physiotherapy (PhyCARE) will be conducted for publication and dissemination. The 2010 \"Guidance for Developers of Health Research Reporting\" and instructions from the Enhancing the QUAlity and Transparency Of health Research (EQUATOR) Network will be followed in the preparation of PhyCARE guidelines. The guidelines will be propagated on different platforms and journals will be requested to adopt the guidelines.</p><p><strong>Registration: </strong>The reporting guideline under development is prospectively registered on the EQUATOR Network website on PhyCaRe - Reporting guideline for physiotherapy case reports.</p>","PeriodicalId":12260,"journal":{"name":"F1000Research","volume":"12 ","pages":"838"},"PeriodicalIF":0.0,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10465994/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10231210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correlation between posterior teeth loss and temporomandibular joint disorder symptoms in adult patients.","authors":"Batool Hussein Ayaz, May Wathiq Al-Khudhairy","doi":"10.12688/f1000research.161006.1","DOIUrl":"10.12688/f1000research.161006.1","url":null,"abstract":"<p><strong>Background/objectives: </strong>The relation between TMD and posterior tooth loss is still up for debate and is a topic of constant discussion. The present study aimed to find a correlation between posterior teeth loss and TMJ disorder symptoms in adult patients.</p><p><strong>Methods: </strong>A total of fifty patients were selected for the study. First, intra-oral dental examination recorded all missing posterior teeth, the teeth numbers, type of missing teeth (except third molars). All existing teeth are examined during the clinical examination to identify any odontogenic causes for pain if present. Second, patients were asked to fill in the Arabic-translated form of the DC/TMD Axis I symptom questionnaire. Third, the DC/TMD Axis II protocol was applied to each patient and the examination form was completed by the examiner during the clinical examination.</p><p><strong>Results: </strong>Disc displacement with reduction is more likely to cause headache, pain, and clicking. A statistically significant positive correlation was found between age and the number of missing teeth. Furthermore, a statistically significant negative correlation was found between age and maximum unassisted mouth opening, and the number of missing teeth and maximum unassisted mouth opening. Logistics regression analysis showed clicking was significantly associated and 13.8 (OR) times more likely to have TMD.</p><p><strong>Conclusions: </strong>The current study reported that patients with TMD are more likely to have pain, headache, clicking, and a decrease in maximum mouth opening. There was a correlation between clicking and TMJ disorder, and the number of tooth loss and TMJ disorder.</p>","PeriodicalId":12260,"journal":{"name":"F1000Research","volume":"14 ","pages":"428"},"PeriodicalIF":0.0,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12120414/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144179804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The influence of the fourth industrial revolution in teaching and learning: the COVID-19 context.","authors":"Awelani V Mudau, Lettah Sikhosana","doi":"10.12688/f1000research.131618.2","DOIUrl":"10.12688/f1000research.131618.2","url":null,"abstract":"<p><strong>Background: </strong>The aim of this paper was to explore how the Fourth Industrial Revolution shapes teaching and learning during COVID-19 in some of the schools located in the Gauteng and Mpumalanga provinces, South Africa. This paper employed qualitative interpretative multiple case study design. Purposive sampling was used to sample our participants.</p><p><strong>Methods: </strong>We selected four teachers who taught at the early childhood development stage, intermediate phase, senior phase, and further education and training phases. Data was collected telephonically through semi-structured interviews with teachers from Limpopo, Gauteng and Mpumalanga provinces of South Africa. Data was collected from their experiences of COVID-19 from 2019 to 2021. Collected data was analysed using a typology approach whereby themes that derived from the literature reviewed and research questions were used.</p><p><strong>Results: </strong>We inferred from the results of the paper that teachers had challenges with teaching and the availability of learning resources, such as limited access to the internet and socio-economic backgrounds, which hindered the integration of the Fourth Industrial Revolution in the teaching and learning process. There were also challenges related to teachers' background on the usage of the Fourth Industrial Revolution and the lack of school management teams. The paper revealed that the integration of the Fourth Industrial Revolution in teaching and learning was affected negatively by the existence of the above-mentioned challenges, which need to be addressed.</p><p><strong>Conclusions: </strong>Therefore, we recommended that the government and stakeholders within the education sector provide resources such as smart-boards, computers, and network access in schools lacking such facilities, as well as providing professional development interventions and training teachers to have an in-depth understanding of the Fourth Industrial Revolution within the teaching and learning context.</p>","PeriodicalId":12260,"journal":{"name":"F1000Research","volume":"12 ","pages":"1183"},"PeriodicalIF":0.0,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12120419/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144181928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A global overview of the use of cone beam computed tomography in dentistry: a bibliometric review focusing on paediatric patients.","authors":"Danielle Cristina Alves Rigo, Aurélio de Oliveira Rocha, Lucas Menezes Dos Anjos, Pablo Silveira Santos, Isabela Ramos, Michely Cristina Goebel, Julia Maldonado Garcia, Gabriela Beatriz Rigo Wietzkoski, Carla Miranda Santana, Mariane Cardoso","doi":"10.12688/f1000research.157349.2","DOIUrl":"https://doi.org/10.12688/f1000research.157349.2","url":null,"abstract":"<p><strong>Background: </strong>Cone Beam Computed Tomography (CBCT) has improved diagnosis and treatment planning in paediatric dentistry, but no bibliometric studies have examined the research landscape. This study provides an overview regarding the role of CBCT in paediatric dentistry.</p><p><strong>Methods: </strong>A bibliometric review was conducted using articles from the Web of Science database. The search was performed on 22 February 2024, including publications up to that date. Conference papers and editorials were excluded. Data extracted included citation counts, publication dates, journals, impact factors, study designs, topics, geographical and institutional affiliations, authors, and keywords. Collaborative networks were visualised using VOSviewer, and Spearman's correlation assessed the relationship between citation counts and other variables.</p><p><strong>Results: </strong>The review analysed 517 articles, with the most cited receiving 557 citations. Publication dates ranged from 2005 to 2024, with a peak in 2023. Observational studies were the most common, particularly on maxillary expansion. The American Journal of Orthodontics and Dentofacial Orthopedics was the most cited journal, and the USA was a major contributor. Jacobs R authored the most articles (n=19), and the University of Alberta led in institutional output. Spearman's correlation showed a weak positive correlation between citation count and journal impact factor (rho=0.272, p<0.001) and a strong negative correlation with publication year (rho=-0.762, p<0.001).</p><p><strong>Conclusions: </strong>This bibliometric review provides an overview of the use of CBCT in paediatric dentistry, particularly in maxillary expansion. The findings suggest that more specific imaging protocols may improve safety and clinical outcomes, and that further investigation of long-term outcomes may provide valuable insights.</p>","PeriodicalId":12260,"journal":{"name":"F1000Research","volume":"13 ","pages":"1320"},"PeriodicalIF":0.0,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12009477/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144005650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mapping the landscape of women entrepreneurs in micro and small enterprises: Trends, themes, and insights through systematic review and text mining.","authors":"Thanh Binh Nguyen, Mohan Kumar, Deepak Kumar Sahoo, Neha Nain, Mohit Yadav, Vartika Dadhich","doi":"10.12688/f1000research.163030.1","DOIUrl":"10.12688/f1000research.163030.1","url":null,"abstract":"<p><strong>Background: </strong>This study explores the pivotal role of women entrepreneurs in fostering economic growth and social transformation, focusing on micro and small enterprises (MSEs). Despite the significant rise in women entrepreneurship, challenges persist, including gender-based barriers, limited access to capital, and regional disparities. Understanding these dynamics is essential for formulating policies that support and empower women entrepreneurs.</p><p><strong>Method: </strong>The present study employed a systematic literature review (SLR) and Latent Dirichlet Allocation (LDA), an advanced topic modeling technique, to identify prevalent themes within the literature. Data was extracted from Scopus-indexed journals (2000-2024), systematically filtered, and analyzed to uncover key patterns in women entrepreneurship research.</p><p><strong>Results: </strong>The analysis identifies four central themes: (1) Women in Entrepreneurship and Gender Roles, (2) Small Business and Women Enterprises, (3) Social Impact of Women Entrepreneurs, and (4) Challenges Faced by Female Entrepreneurs. The findings highlight the transformative potential of digital platforms in enhancing business opportunities for women and addressing traditional constraints, such as mobility and market access.</p><p><strong>Conclusion: </strong>This study highlights the need for targeted interventions to enhance digital literacy, bridge socio-cultural disparities, and establishing inclusive policies that foster a supportive entrepreneurial ecosystem. By synthesizing existing literature, this research provides actionable recommendations for policymakers, financial institutions, and stakeholders to strengthen women-led enterprises and drive gender-equitable growth.</p>","PeriodicalId":12260,"journal":{"name":"F1000Research","volume":"14 ","pages":"422"},"PeriodicalIF":0.0,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12153045/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144274590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quantized Convolutional Neural Networks Robustness under Perturbation.","authors":"Jack Langille, Issam Hammad, Guy Kember","doi":"10.12688/f1000research.163144.1","DOIUrl":"https://doi.org/10.12688/f1000research.163144.1","url":null,"abstract":"<p><p>Contemporary machine learning models are increasingly becoming restricted by size and subsequent operations per forward pass, demanding increasing compute requirements. Quantization has emerged as a convenient approach to addressing this, in which weights and activations are mapped from their conventionally used floating-point 32-bit numeric representations to lower precision integers. This process introduces significant reductions in inference time and simplifies the hardware requirements. It is a well-studied result that the performance of such reduced precision models is congruent with their floating-point counterparts. However, there is a lack of literature that addresses the performance of quantized models in a perturbed input space, as is common when stress testing regular full-precision models, particularly for real-world deployments. We focus on addressing this gap in the context of 8-bit quantized convolutional neural networks (CNNs). We study three state-of-the-art CNNs: ResNet-18, VGG-16, and SqueezeNet1_1, and subject their floating point and fixed point forms to various noise regimes with varying intensities. We characterize performance in terms of traditional metrics, including top-1 and top-5 accuracy, as well as the F1 score. We also introduce a new metric, the Kullback-Liebler divergence of the two output distributions for a given floating-point/fixed-point model pair, as a means to examine how the model's output distribution has changed as a result of quantization, which, we contend, can be interpreted as a proxy for model similarity in decision making. We find that across all three models and under each perturbation scheme, the relative error between the quantized and full-precision model was consistently low. We also find that Kullback-Liebler divergence was on the same order of magnitude as the unperturbed tests across all perturbation regimes except Brownian noise, where significant divergences were observed for VGG-16 and SqueezeNet1_1.</p>","PeriodicalId":12260,"journal":{"name":"F1000Research","volume":"14 ","pages":"419"},"PeriodicalIF":0.0,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12041843/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144005229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of sub-maximal aerobic capacity in North African patients with chronic hepatitis B: a pilot case-control study.","authors":"Jihene Bergaoui, Imed Latiri, Sawssen Mrad, Houda Chaouch, Salma Amous, Jihene Ben Abdallah, Samia Ernez Hajri, Helmi Ben Saad","doi":"10.12688/f1000research.160390.1","DOIUrl":"https://doi.org/10.12688/f1000research.160390.1","url":null,"abstract":"<p><strong>Background: </strong>Studies assessing sub-maximal aerobic capacity in non-cirrhotic chronic hepatitis B (CHB) patients are scarce. This study aimed to evaluate sub-maximal aerobic capacity in CHB patients compared to apparently healthy participants (control-group (CG)).</p><p><strong>Methods: </strong>A 6-min walk test (6MWT) was performed. The 6-min walk distance (6MWD) was recorded, along with heart-rate (HR), oxy-hemoglobin saturation (SpO ₂), blood-pressure, and dyspnea ( <b><i>ie</i></b> ; visual analogue scale) at rest (Rest) and at the end (End) of the 6MWT. Additionally, the 6-min walk work (6MWW), and estimated cardiorespiratory and muscular chain age were calculated. Signs of physical intolerance were determined including abnormal 6MWD ( <b><i>ie</i></b> ; 6MWD < lower limit of normal), chronotropic insufficiency (ie ; HREnd < 60% of maximal predicted HR (MPHR)), high dyspnea ( <b><i>ie</i></b> ; dyspneaEnd > 5), and desaturation ( <b><i>ie</i></b> ; drop in SpO ₂ > 5 points).</p><p><strong>Results: </strong>Compared to the CG (n=28), the CHB-group (n=26) exhibited significantly lower 6MWD by 61 meters (8%), lower 6MWW by 10%, and lower HR _End by 21% (when expressed in bpm) and 17% (when expressed in %MPHR). The CHB-group, compared to the CG, included higher percentages of participants with chronotropic insufficiency and abnormal 6MWD (23.08% vs. 3.57%, and 34.61% vs. 3.57%, respectively). The CHB-group was 8.1 and 14.3 times more likely to have chronotropic insufficiency and abnormal 6MWD than the CG, respectively. CHB accelerated the aging of the cardiorespiratory and muscular chain by 11 years.</p><p><strong>Conclusion: </strong>Non-cirrhotic CHB may contribute to reduced submaximal aerobic capacity and acceleration of cardiorespiratory and muscular chain aging.</p>","PeriodicalId":12260,"journal":{"name":"F1000Research","volume":"14 ","pages":"98"},"PeriodicalIF":0.0,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12022957/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143992353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact on postoperative complications of combined prehabilitation targeting co-existing smoking, malnutrition, obesity, alcohol drinking, and physical inactivity: a systematic review and meta-analysis of randomised trials.","authors":"Line N Lydom, Sofie Anne-Marie S Jensen, Susanne V Lauridsen, Mette Rasmussen, Robin Christensen, Ulla N Joensen, Jacob Rosenberg, Hanne Tønnesen","doi":"10.12688/f1000research.150880.2","DOIUrl":"10.12688/f1000research.150880.2","url":null,"abstract":"<p><strong>Background: </strong>This study aimed to compare the effect on postoperative complications of combined prehabilitation targeting predefined co-existing risky SNAP factors with usual preoperative routines in surgical patients.</p><p><strong>Methods: </strong>This systematic review followed the PRISMA 2020 guideline and the protocol (CRD42022282611). Five databases were searched from inception to November 7, 2022 for randomised controlled trials on prehabilitation targeting ≥2 predefined risky lifestyles compared with usual preoperative routines. Risky lifestyles included Smoking, Nutrition (malnutrition and/or BMI>25), risky Alcohol intake, and Physical inactivity (SNAP). Primary outcome was postoperative complications ≤30 days. Cochrane's risk-of-bias tool 2 was used and meta-analyses were conducted. GRADE was used to assess certainty of evidence.</p><p><strong>Results: </strong>The search resulted in 20,862 records. At full-text screening, only two (120 participants) of 24 identified trials on combined SNAP intervention had ≥2 predefined risk factors and were included. One (n=110) on intensive physical and brief nutritional intervention to frail patients with colorectal cancer resection reported complication rates of 45% in both groups (relative risk (RR) 1.00, 95% CI 0.66 to 1.51). The other study (n=10, subgroup) on intensive alcohol and smoking intervention in patients with bladder cancer undergoing radical cystectomy, reported complications in 3/7 vs 3/3 participants. The meta-analysis estimated a RR of 0.79 (95% CI 0.41 to 1.51, I ² 51%).</p><p><strong>Conclusion: </strong>Two small of the 24 trials on prehabilitation targeted co-existing and predefined risky SNAP factors and the effect on postoperative complications is very uncertain. Future prehabilitation research involving patient needs is warranted.</p>","PeriodicalId":12260,"journal":{"name":"F1000Research","volume":"13 ","pages":"694"},"PeriodicalIF":0.0,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12198731/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144505283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First Report of <i>Ganoderma ryvardenii</i> causing Basal Stem Rot (BSR) disease on oil palm ( <i>Elaeis guineensis</i> Jacq.) in Ghana.","authors":"Emmanuellah Lekete-Lawson, Grace C van der Puije, Enoch A Osekre, Frank K Ackah","doi":"10.12688/f1000research.161972.1","DOIUrl":"10.12688/f1000research.161972.1","url":null,"abstract":"<p><strong>Backgrounds: </strong>Oil palm ( <i>Elaeis guineensis</i> Jacq.), is the most significant and highest-yielding crop among oil-producing crops worldwide. In 2020/2022, Basal stem rot (BSR) disease was observed in six oil palm growing Districts in Ghana.</p><p><strong>Methods: </strong>Field study and laboratory analysis were conducted. A random sampling technique was used to select five plantation blocks from each District. Single-point disease assessments were done using Standard Operating Procedure (SOP) with a severity scale of 0-4. Molecular assays were performed on each sample using nucleic acid as a template. ITS and GanET sequence analysis were performed along with the formation of a phylogenetic tree using the FASTA algorithm with the Fungus database from EBI and NCBI GenBank. Koch's postulate was followed to confirm the disease.</p><p><strong>Results: </strong>The disease incidence was 11.3 % with the highest severity score of 4. BSR is characterised by stem decay large-perennial, woody brackets basidiocarps of average measurement of 2-65 cm in diameter on infected palms. Culture colonies were white, striated, undulating, woolly-cottony, and creamish pigment on the reverse depicting attributes of <i>Ganoderma</i> fungus. Molecular confirmation was done by combining ITS sequence of top matches of >97% to members of the genus <i>Ganoderma</i>, >98% and 99.3% identity to three sequences of <i>Ganoderma</i> sp. (HM138671; HM138670 and HM138672) generated from strains assigned to <i>Ganoderma ryvardenii</i> and compared with 132 published sequences of <i>Ganoderma</i> isolates.</p><p><strong>Conclusion: </strong>This is the first report of <i>Ganoderma ryvardenii</i> causing BSR disease on oil palm in Ghana and possibly the second report in Africa. However, the pathogen was first reported to cause similar diseases in oil palm in Cameroon.</p>","PeriodicalId":12260,"journal":{"name":"F1000Research","volume":"14 ","pages":"413"},"PeriodicalIF":0.0,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12125568/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144198644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diversity, Distribution, and Phenotypic Characterization of Cultivable Wild Yeasts Isolated from Natural Forest.","authors":"Teshome Tadesse, Degife Dese, Anbessa Dabassa, Ketema Bacha","doi":"10.12688/f1000research.160250.3","DOIUrl":"https://doi.org/10.12688/f1000research.160250.3","url":null,"abstract":"<p><strong>Background: </strong>Yeasts are unicellular fungi that inhabit a variety of environments including plant surfaces, water, soil, and animal hosts. However, limited research has been conducted on soil and plant associated yeasts in Africa, with most studies originating from developed regions.</p><p><strong>Methods: </strong>This study explored the diversity, distribution, and phenotypic characterization of cultivable wild yeast in samples from rhizosphere soil, leaves, litter, and tree bark collected from South West Ethiopia. Yeast isolates were characterized using morphological, physiological and biochemical methods, Stress-tolerant yeast species were identified using Matrix-Assisted Laser Desorption Ionization-Time of Flight (MALDI-TOF).</p><p><strong>Results: </strong>Based on morphological, physiological, and biochemical analyses, a total of 15 yeast genera were identified from 23 plant species. Predominant yeast species included <i>Candida</i> spp., <i>Saccharomyces</i> spp., <i>Meyerozyma</i> spp., <i>Pichia</i> spp., <i>Geotrichum</i> spp., and <i>Hanseniaspora</i> spp. Plant species with the highest yeast diversity were <i>Ficus vasta</i>, <i>Ficus exasperata</i>, <i>Ficus sycomorus</i>, <i>Cordia africana</i>, and <i>Ritchiea albersii.</i> Bark samples yielded more yeast isolates than rhizosphere soil, litter, and leaves. Stress-tolerant species such as <i>Saccharomyces cerevisiae</i>, <i>Candida pelliculosa</i>, <i>Meyerozyma guilliermondii</i>, <i>Pichia kluyveri</i>, and <i>Trichosporon asahii</i> were identified using MALDI-TOF. Correlation analysis revealed no significant relationship between yeast populations in bark and leaf samples or between rhizosphere soil and leaves, though a weak positive correlation was found between rhizosphere soil and bark or litter. Seasonal analysis showed a strong positive correlation between yeast abundance in spring and summer, but no association between autumn and spring.</p><p><strong>Conclusion: </strong>Ethiopian forests are home for various yeast species including the stress-tolerant wild yeasts. This study highlights the significant yeast diversity in Ethiopian forests, with potential applications in improving industrial fermentation processes that operate under stressful conditions.</p>","PeriodicalId":12260,"journal":{"name":"F1000Research","volume":"14 ","pages":"105"},"PeriodicalIF":0.0,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12033980/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144000587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}