F1000Research最新文献

{"title":"A guide to selecting high-performing antibodies for CSNK2A1 (UniProt ID: P68400) for use in western blot, immunoprecipitation and immunofluorescence.","authors":"Riham Ayoubi, Maryam Fotouhi, Charles Alende, Vera Ruíz Moleón, Kathleen Southern, Carl Laflamme","doi":"10.12688/f1000research.153243.2","DOIUrl":"https://doi.org/10.12688/f1000research.153243.2","url":null,"abstract":"<p><p>Casein kinase II subunit alpha (CSNK2A1), a serine/threonine kinase, phosphorylates multiple protein substrates and is involved in diverse cellular and biological processes. Implicated in various human diseases, high-performing antibodies would help evaluate its potential as a therapeutic target and benefit the scientific community. In this study, we have characterized ten CSNK2A1 commercial antibodies for western blot, immunoprecipitation, and immunofluorescence using a standardized experimental protocol based on comparing read-outs in knockout cell lines and isogenic parental controls. These studies are part of a larger, collaborative initiative seeking to address antibody reproducibility issues by characterizing commercially available antibodies for human proteins and publishing the results openly as a resource for the scientific community. While use of antibodies and protocols vary between laboratories, we encourage readers to use this report as a guide to select the most appropriate antibodies for their specific needs.</p>","PeriodicalId":12260,"journal":{"name":"F1000Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11472280/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142461517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>In Vitro</i> Alpha-Glucosidase Inhibitory Effect of <i>Etlingera Elatior</i> Ethanol Extract Growing in Gayo Highland, Aceh Province, Indonesia.","authors":"Zumaidar Zumaidar, Nuzul Asmilia, Saudah Saudah, Milda Husnah","doi":"10.12688/f1000research.149029.2","DOIUrl":"10.12688/f1000research.149029.2","url":null,"abstract":"<p><strong>Background: </strong>The prevalence of diabetes mellitus (DM) is increasing overtime, potentially leading to various severe health complications and mortality. Despite therapeutic agents have currently been developed, unexpected adverse effects are inevitable. Hence, safe and effective medications such as those of plant origin are critical to prevent unexpected complication in DM sufferers. <i>Etlingera elatior</i> has been widely used as spice and traditional medicine to treat diabetes in Aceh Province, Indonesia. However, study regarding α-glucosidase inhibitory effect of <i>E. elatior</i> growing in Gayo highlands, Aceh, Indonesia, is completely lacking. The aim of this study was to evaluate <i>in vitro</i> α-glucosidase inhibitory effect of <i>E. elatior</i> ethanol extracts (EEEE) growing in Gayo highlands, Aceh Province, Indonesia.</p><p><strong>Methods: </strong>Antioxidant activity was determined using DPPH procedure, whereas α-glucosidase inhibition assay was carried out using spectrophotometric method. Data analysis was performed using One-Way Analysis of Variance (ANOVA), followed by Duncan's multiple range test at α=0.05.</p><p><strong>Results: </strong>Phytochemical analysis revealed the presence of total phenolic (TPC), total flavonoid (TFC), and total tannin (TTC) content in all <i>E. elatior</i> plant parts, in which the highest TPC was found in the stem (158.38 GAE/g), whereas the highest TFC and TTC was obtained in the rhizome extracts. The extract of fruit showed the strongest antioxidant activities, followed by the stem and leaf, with IC ₅₀ of 2.381 μg/mL, 6.966 μg/mL, and 19.365 μg/mL, respectively. All <i>E. elatior</i> extracts revealed a significant inhibitory activity against α-glucosidase at the concentration of 500 μg/mL, in which the stem extract showed the most effective α-glucosidase inhibitory effect with IC ₅₀ value of 5.15 μg/mL, suggesting its promising potential as antidiabetic agent.</p><p><strong>Conclusions: </strong>This study highlights <i>E. elatior</i> potency as a novel source of antioxidant and natural antidiabetic compounds that are useful for the prevention and treatment of diabetes.</p>","PeriodicalId":12260,"journal":{"name":"F1000Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11487234/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142461504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Dynamic, D-dimer-based Thromboprophylaxis Strategy in Patients with COVID-19.","authors":"Lantarima Bhoopat, Anastasia Martynova, April Choi, Pattharawin Pattharanitima, Semi Han, Senxi Du, Ibrahim Syed, Catherine Chan, Esther E Oh, Zea Borok, Janice Liebler, Melissa Lee Wilson, Pichaya Tantiyavarong, Casey O Connell","doi":"10.12688/f1000research.146710.2","DOIUrl":"https://doi.org/10.12688/f1000research.146710.2","url":null,"abstract":"<p><strong>Background: </strong>COVID-19 pandemics increases venous thromboembolism (VTE) risk during hospitalization, despite prophylactic anticoagulation. Limited radiological diagnosis in pandemic requires a guided protocol for anticoagulant adjustment.</p><p><strong>Methods: </strong>This retrospective cohort study was conducted at a single center as part of a quality improvement program evaluating the efficacy and safety of anticoagulation protocols. The study focused on implementing a guideline for anticoagulant dosing protocol based on dynamic changes in D-dimer levels in COVID-19 hospitalized patients. The dosing guideline allowed for dose escalation from standard prophylactic levels to escalated prophylactic or therapeutic levels, depending on the patient's risk profile for VTE. The primary endpoints included in-hospital survival comparing between fix and dynamic adjustment treatment groups. Secondary endpoints encompassed major and clinically relevant non-major bleeding (CRNMB) events, incidence of breakthrough thrombosis, length of hospitalization and ICU stay, days of mechanical ventilator use, and survival duration.</p><p><strong>Findings: </strong>Among the 260 COVID-19-infected patients hospitalized between March 15th and June 15th, 2020. The patients received fixed anticoagulant dosage in 188, 72.3%) patients, while 72 (27.7%) were up-titrated according to the protocol. In-hospital survival at 30 days demonstrated superiority among patients whose anticoagulation was up-titrated to either escalated prophylactic or therapeutic (80.2%) compared to receiving fixed anticoagulant dosage (51.3%) (p=0.01). Bleeding events were significantly higher in up-titrate group (12.5%) compared to fixed anticoagulant dosage group (2.13%). Most of them are CRNMB.</p><p><strong>Conclusion: </strong>A dynamic, D-dimer-based dose escalation of anticoagulation for hospitalized patients with COVID-19 holds promise in improving in-hospital mortality rates without a significant increase in fatal bleeding events.</p>","PeriodicalId":12260,"journal":{"name":"F1000Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11467651/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142461505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The rise of pathogen genomics in Africa.","authors":"Gerald Mboowa, Francis Kakooza, Moses Egesa, Stephen Tukwasibwe, Stephen Kanyerezi, Ivan Sserwadda, Benson R Kidenya, Jupiter Marina Kabahita, Maria Magdalene Namaganda, Mike Nsubuga, Patricia Nabisubi, Alisen Ayitewala, Grace Kebirungi, Esther Nakafu, Natasha Patience Akwii","doi":"10.12688/f1000research.147114.2","DOIUrl":"10.12688/f1000research.147114.2","url":null,"abstract":"<p><p>The routine genomic surveillance of pathogens in diverse geographical settings and equitable data sharing are critical to inform effective infection control and therapeutic development. The coronavirus disease 2019 (COVID-19) pandemic highlighted the importance of routine genomic surveillance of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) to detect emerging variants of concern. However, the majority of high-income countries sequenced >0.5% of their COVID-19 cases, unlike low- and middle-income countries. By the end of 2022, many countries around the world had managed to establish capacity for pathogen genomic surveillance. Notably, Beta and Omicron; 2 of the 5 current SARS-CoV-2 variants of concern were first discovered in Africa through an aggressive sequencing campaign led by African scientists. To sustain such infrastructure and expertise beyond this pandemic, other endemic pathogens should leverage this investment. Therefore, countries are establishing multi-pathogen genomic surveillance strategies. Here we provide a catalog of the current landscape of sequenced and publicly shared pathogens in different countries in Africa. Drawing upon our collective knowledge and expertise, we review the ever-evolving challenges and propose innovative recommendations.</p>","PeriodicalId":12260,"journal":{"name":"F1000Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11512136/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142497712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Feasibility Study of Community-Based Training for Musculoskeletal Health Promotion.","authors":"Rizky Suganda Prawiradilaga, Magnus Bendtsen, Simon Esrup, Niklas Rye Jørgensen, Fajar Awalia Yulianto, Eva Wulff Helge","doi":"10.12688/f1000research.73698.3","DOIUrl":"https://doi.org/10.12688/f1000research.73698.3","url":null,"abstract":"<p><p><b>Background:</b> To minimize fracture risk, multimodal training regimens are recommended. However, their effectiveness in community settings remains uncertain. This study evaluated the feasibility of 19-weeks of multimodal training in a local community center with emphasis on musculoskeletal health in postmenopausal women. <b>Methods:</b> In a controlled trial, 28 postmenopausal women (53-68-years-old) were assigned to a multimodal training group (MMT, n=15) or a control group (CON, n=13). The training consisted of high- and odd-impact, resistance and balance-coordination training 1-2 hours weekly. The outcomes were attendance rate, regional and total bone mineral density (BMD), bone mineral content (BMC), bone turnover markers (BTM), body composition, functional muscle strength and power, and dynamic balance. All were determined at baseline and after 19 weeks of training. BTM was assessed after three weeks. <b>Results:</b> Overall, 22(79%) participants (MMT, n=9; CON, n=13) completed the study, and the mean attendance rate for MMT was 65.5% of the maximum sessions (2) offered. Only right trochanter BMD increased (p<0.05) by 1.0±1.1% in MMT, which was higher(p<0.05) than CON. While whole-body BMC was not changed at 19 weeks from baseline in MMT, it decreased (p<0.05) in CON resulting in a significant difference (p<0.05) in whole-body BMC delta values between the two groups. Compared to baseline, body fat percentage(%BF), fat mass(FM), and visceral adipose tissue (VAT)-mass and -volume were decreased (p<0.01) in MMT, and were larger (p<0.05) than CON. No significant changes were observed in BTM, muscle strength and power, and dynamic balance after 19 weeks. <b>Conclusions:</b> Nineteen weeks of multimodal training 1-2 hours per week in a local community had a health-enhancing effect on %BF, FM, and VAT, whereas the musculoskeletal health impact was modest. We hypothesize that the reason might be too low training volume and frequency and supposedly too low musculoskeletal training intensity for some participants. <b>Registration:</b> ClinicalTrials.gov NCT05164679 (21/12/2021).</p>","PeriodicalId":12260,"journal":{"name":"F1000Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11387933/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142282714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Teaching critical thinking about health information and choices in secondary schools: human-centred design of digital resources.","authors":"Sarah Rosenbaum, Jenny Moberg, Faith Chesire, Michael Mugisha, Ronald Ssenyonga, Marlyn A Ochieng, Clarisse Marie Claudine Simbi, Esther Nakyejwe, Benson Ngatia, Gabriel Rada, Juan Vásquez-Laval, José Damián Garrido, Grace Baguma, Sam Kuloba, Edward Sebukyu, Richard Kabanda, Irene Mwenyango, Tonny Muzaale, Pamela Nandi, Jane Njue, Cyril Oyuga, Florian Rutiyomba, Felecien Rugengamanzi, Joan Murungi, Allen Nsangi, Daniel Semakula, Margaret Kaseje, Nelson Sewankambo, Laetitia Nyirazinyoye, Simon Lewin, Andrew D Oxman, Matt Oxman","doi":"10.12688/f1000research.132580.3","DOIUrl":"10.12688/f1000research.132580.3","url":null,"abstract":"<p><strong>Background: </strong>Learning to thinking critically about health information and choices can protect people from unnecessary suffering, harm, and resource waste. Earlier work revealed that children can learn these skills, but printing costs and curricula compatibility remain important barriers to school implementation. We aimed to develop a set of digital learning resources for students to think critically about health that were suitable for use in Kenyan, Rwandan, and Ugandan secondary schools.</p><p><strong>Methods: </strong>We conducted work in two phases collaborating with teachers, students, schools, and national curriculum development offices using a human-centred design approach. First, we conducted context analyses and an overview of teaching strategies, prioritised content and collected examples. Next, we developed lessons and guidance iteratively, informed by data from user-testing, individual and group interviews, and school pilots.</p><p><strong>Results: </strong>Final resources include online lesson plans, teachers' guide, and extra resources, with lesson plans in two modes, for use in a classroom equipped with a blackboard/flip-chart and a projector. The resources are accessible offline for use when electricity or Internet is lacking. Teachers preferred the projector mode, as it provided structure and a focal point for class attention. Feedback was largely positive, with teachers and students appreciating the learning and experiencing it as relevant. Four main challenges included time to teach lessons; incorrect comprehension; identifying suitable examples; and technical, logistical, and behavioural challenges with a student-computer mode that we piloted. We resolved challenges by simplifying and combining lessons; increasing opportunities for review and assessment; developing teacher training materials, creating a searchable set of examples; and deactivating the student-computer mode.</p><p><strong>Conclusion: </strong>Using a human-centred design approach, we created digital resources for teaching secondary school students to think critically about health actions and for training teachers. Be smart about your health resources are open access and can be translated or adapted to other settings.</p>","PeriodicalId":12260,"journal":{"name":"F1000Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11377934/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142153542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring antioxidant potential of agricultural by-products: a systematic review.","authors":"Imam Santoso, Suprayogi Suprayogi, Akhmad Adi Sulianto, Endrika Widyastuti, Annisa'U Choirun, Khairunnisa Lestari, Syairil A'yuniah, Octavia Widyastuti Kusumaningtyas","doi":"10.12688/f1000research.145702.1","DOIUrl":"https://doi.org/10.12688/f1000research.145702.1","url":null,"abstract":"<p><strong>Background: </strong>Agricultural waste sourced from various activities that occur along the agricultural supply chain including post-harvest, processing, and consumption processes, can pose a threat to ecosystem balance and community welfare. Data shows that agricultural by-products have the potential to be utilized because they contain antioxidant compounds. This systematic review study aims to identify and assess the antioxidant activity of agricultural by-products through various extraction methods.</p><p><strong>Methods: </strong>This systematic review collected literature in the last 10 years (2013-2023) from Google Scholar, Semantic, and Scopus-indexed articles with the help of Publish or Perish. Using the help of boolean operators (AND) and (OR) in searching using keywords. The steps applied adapt the PRISMA method (Preferred Reporting Items for Systematic Reviews and Meta-Analyses), including identification, screening, eligibility, and inclusion.</p><p><strong>Results: </strong>Literature collection data shows that the dominant processing method used is the solvent extraction method to determine the antioxidant value of various agricultural waste by-products. Followed by microwave-assisted extraction (MAE) and ultrasound-assisted extraction (UAE) methods. A wide range of antioxidant activity values were found depending on the type of agricultural waste and processing technique. One potential utilization of agricultural wastes rich in antioxidant content is as additives in formulations in the cosmetic industry.</p><p><strong>Conclusion: </strong>Agricultural waste by-products have high potential of antioxidant content, depending on the type of waste and extraction method. The dominant agricultural waste used is by-products from the fruit group. The utilization of agricultural waste that is rich in antioxidants has the potential to be utilized in the cosmetic industry.</p>","PeriodicalId":12260,"journal":{"name":"F1000Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11474157/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142461538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Socio-demographic analysis of destination selection factors for Himalayan Hill destinations.","authors":"Manish Badoni, Babita Rawat, Megha Aggarwal","doi":"10.12688/f1000research.146873.2","DOIUrl":"https://doi.org/10.12688/f1000research.146873.2","url":null,"abstract":"<p><strong>Background: </strong>The towering peaks of the Himalayas lie in troves of captivating hill destinations, especially in India. Each destination aims to provide tourists with unique experiences and breath-taking landscapes. Understanding the tapestry of factors that weave the allure of these destinations and draw visitors from diverse backgrounds remains intriguing.</p><p><strong>Method: </strong>This study delves into the socio-demographic tapestry of Himalayan hill destination selection, unraveling the complex interplay of demographic characteristics, social influences, and individual motivations that shape tourists' choices.</p><p><strong>Results: </strong>This study aims to answer why different tourists have different travel choices and what factors are the drivers behind such choices. The results show that destination selection factors are similar irrespective of respondents' socio-demographic variabilities; however, for a few factors, the results are reversed.</p><p><strong>Conclusion: </strong>The study has implications for policymakers and the limitations of the research discussed at the end.</p>","PeriodicalId":12260,"journal":{"name":"F1000Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11375401/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142139699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Case Report: <i>Shewanella algae</i>, a rare cause of osteosynthesis-associated infection.","authors":"Sofiane Masmoudi, Mohamed Ali Khlif, Hajer Battikh, Meriam Zribi, Maher Barsaoui, Khaled Zitouna","doi":"10.12688/f1000research.142096.2","DOIUrl":"10.12688/f1000research.142096.2","url":null,"abstract":"<p><p><i>Shewanella</i> is an emerging human pathogen. It mostly causes skin and soft tissue infections. Osteosynthesis-associated infection involving <i>Shewanella</i> are rare and in most cases are secondary to direct contamination following open fractures in aquatic environments. Here, we present a rare case of hematogenous osteosynthesis-associated infection involving <i>Shewanella algae</i> affecting an 18-year-old patient who was operated on for 12 ^th thoracic vertebrae and 4th lumbar vertebrae fractures occurring in an aquatic environment. We performed surgical debridement with subsequent double course parenteral antibiotherapy that was then adapted to bacteria sensitivities for three weeks. After a follow-up of six months, the patient had no signs of recurrent infection. The presence of infected dermabrasions and the concordance between germs isolated in operative samples and in blood cultures presumes that the contamination was hematogenous.</p>","PeriodicalId":12260,"journal":{"name":"F1000Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11375409/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142139765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clust&See3.0 : clustering, module exploration and annotation.","authors":"Fabrice Lopez, Lionel Spinelli, Christine Brun","doi":"10.12688/f1000research.152711.1","DOIUrl":"10.12688/f1000research.152711.1","url":null,"abstract":"<p><strong>Background: </strong>Cytoscape is an open-source software to visualize and analyze networks. However, large networks, such as protein interaction networks, are still difficult to analyze as a whole.</p><p><strong>Methods: </strong>Here, we propose Clust&See3.0, a novel version of a Cytoscape app that has been developed to identify, visualize and manipulate network clusters and modules. It is now enriched with functionalities allowing custom annotations of nodes and computation of their statistical enrichments.</p><p><strong>Results: </strong>As the wealth of multi-omics data is growing, such functionalities are highly valuable for a better understanding of biological module composition, as illustrated by the presented use case.</p><p><strong>Conclusions: </strong>In summary, the originality of Clust&See3.0 lies in providing users with a complete tool for network clusters analyses: from cluster identification, visualization, node and cluster annotations to annotation statistical analyses.</p>","PeriodicalId":12260,"journal":{"name":"F1000Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11528184/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142570956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}