F1000Research最新文献

{"title":"Ozone water or chloroxylenol: The comparison of disinfection effectiveness against the number of bacterial colonies in dental extraction instruments at the Universitas Sumatera Utara Dental and Oral Hospital.","authors":"Hendry Rusdy, Rahmi Syaflida Dalimunthe, Ahyar Riza, Ismahani Akilah D","doi":"10.12688/f1000research.132941.4","DOIUrl":"https://doi.org/10.12688/f1000research.132941.4","url":null,"abstract":"<p><strong>Background: </strong>The application of disinfectants on dental instruments is one way to prevent cross-infection. The urgency in this research is ozone water as a disinfectant is still limited and no research has been found on the use of ozone water on tooth extraction instruments. However, ozone has antibacterial properties so researchers are interested in studying the effectiveness of ozone water as a disinfectant when compared to the commonly used 4.8% chloroxylenol. This study aimed to determine the effectiveness of disinfection of ozone water and 4.8% chloroxylenol in reducing the number of bacterial colonies on dental extraction instruments at the Universitas Sumatera Utara Dental and Oral Hospital.</p><p><strong>Methods: </strong>The samples used were mandibular molar forceps have been used in tooth extraction procedures. This study was experimental and used 30 sample, where each group consisted of 10 tooth extraction instruments. The treatment group used ozone water and 4.8% chloroxylenol and the negative control group was cleaned with distilled water. The ozone water group was produced with an ozone generator with a concentration of 15 mg/L for 20 minutes. Then the active side of tooth extraction forceps was soaked for 30 minutes. In the 4.8% chloroxylenol (dettol®) group, the active side of the mandibular molar tooth extraction forceps was soaked in 250 mL for 60 minutes. In the control group, the mandibular molar tooth extraction forceps were rinsed with distilled water. After that, the sample was counted using a bactery colony counter.</p><p><strong>Results: </strong>The results of the data analysis showed a p-value ≤0.001, which means there was a significant difference in the disinfection using ozone water and 4.8% chloroxylenol on the number of bacterial colonies on dental extraction instruments.</p><p><strong>Conclusions: </strong>From this study it can be said that ozone water is more effective in disinfecting than 4.8% chloroxylenol.</p>","PeriodicalId":12260,"journal":{"name":"F1000Research","volume":"12 ","pages":"726"},"PeriodicalIF":0.0,"publicationDate":"2025-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12374152/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145000001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Experiential retailing in cultural spaces: A case study of multisensory design in batik boutiques.","authors":"Eri Naharani Ustazah, Purwanita Setijanti, Arina Hayati","doi":"10.12688/f1000research.163519.2","DOIUrl":"10.12688/f1000research.163519.2","url":null,"abstract":"<p><strong>Background: </strong>This study examines the role of multisensory design in shaping the spatial atmosphere of batik boutiques and its impact on consumer experience and purchasing behavior. Unlike conventional retail spaces, batik boutiques offer culture-based products for a specialized consumer segment, where shopping involves a highly immersive sensory experience. By analyzing sight, sound, touch, smell, and taste, this research explores how sensory stimuli influence emotional engagement, consumer perception, and decision-making within experiential boutique environments.</p><p><strong>Methods: </strong>This research adopts a qualitative case study approach to analyze experiential retailing in batik boutiques as a brand differentiation strategy. Using an intersubjective paradigm, the study explores sociocultural consumer engagement through semi-structured interviews, direct observations, and photo-elicitation techniques. Data were collected over six months in a batik boutique in East Java, Indonesia, involving purposively selected participants to capture long-term consumer interactions with the store atmosphere and sensory elements influencing their shopping experiences.</p><p><strong>Result and conclusions: </strong>The findings indicate that batik boutiques serve beyond commercial spaces, offering culturally rich, multisensory experiences that enhance consumer attachment, emotional connection, and brand loyalty. Sensory engagement through lighting, music, scent, and tactile interactions significantly impacts consumer perception and purchase behavior. Additionally, nostalgia, identity projection, and social interaction reinforce prolonged engagement, with retail spaces functioning as cultural hubs for heritage appreciation. This study highlights the importance of sensory-driven retail design, demonstrating how store atmosphere influences consumer emotions, cognitive responses, and purchasing intentions within experiential fashion retailing.</p>","PeriodicalId":12260,"journal":{"name":"F1000Research","volume":"14 ","pages":"506"},"PeriodicalIF":0.0,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12171720/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144316278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Greenspace exposure and associated health outcomes: a systematic review of reviews.","authors":"Brittnee Bryer, Chinonso Christian Odebeatu, Wen Ray Lee, Kathryn Vitangcol, Victor Gallegos-Rejas, Nicholas J Osborne, Gail Williams, Darsy Darssan","doi":"10.12688/f1000research.148878.2","DOIUrl":"10.12688/f1000research.148878.2","url":null,"abstract":"<p><strong>Background: </strong>Numerous systematic reviews and meta-analyses have sought to clarify the relationship between greenspace exposure and health outcomes, but the results are inconsistent. We aimed to synthesise all relevant systematic reviews and meta-analyses on this association.</p><p><strong>Methods: </strong>We searched five databases (PubMed, Embase, the Cumulative Index to Nursing and Allied Health Literature [CINAHL], Scopus, and the Cochrane Database of Systematic Reviews) and conducted a manual reference search for systematic reviews and meta-analyses written in English and published in peer-reviewed journals that clearly defined measures of greenspace exposure and reported health outcomes directly attributable to greenspace exposure. A total of 36 systematic reviews published between January 2010 and December 2020 were identified for inclusion in this systematic review of reviews (PROSPERO: CRD42021227422). The methodological quality and risk of bias of included systematic reviews were evaluated by two independent reviewers.</p><p><strong>Results: </strong>Beneficial effects of greenspace exposure were observed for all-cause and cause-specific mortality, as well as mental health and cognitive function. Ambivalent results were found for cardiovascular and metabolic health, general health and quality of life (QOL), and respiratory health and allergies. Most of the systematic reviews included in the current umbrella review had a low to moderate methodological quality and a high risk of bias.</p><p><strong>Conclusions: </strong>This umbrella review highlights the link between greenspaces and a variety of health outcomes, emphasising the importance of preserving existing greenspaces and integrating additional vegetation into urban areas to maintain public health.</p>","PeriodicalId":12260,"journal":{"name":"F1000Research","volume":"13 ","pages":"491"},"PeriodicalIF":0.0,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12426596/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145063816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Primary Research on Correlation between Erectile Function Assessment through International Index of Erectile Function Score and Nocturnal Penile Tumescence and Rigidity Measurements in Men with Erectile Dysfunction.","authors":"Roberto Bagaskara Indy Christanto, Cindy Wijaya, Ponco Birowo, Widi Atmoko, Gerhard Reinaldi Situmorang, Ashok Agarwal, Rupin Shah","doi":"10.12688/f1000research.149499.2","DOIUrl":"https://doi.org/10.12688/f1000research.149499.2","url":null,"abstract":"<p><strong>Introduction: </strong>Erectile dysfunction (ED) affects approximately 40% of men; however, the true prevalence remains uncertain owing to various factors. Diagnosing ED is challenging, and tools like the International Index of Erectile Function (IIEF) and its shorter version, the IIEF-5, are commonly used to assess its severity. Although nocturnal penile tumescence and rigidity (NPTR) monitoring, as an objective test, can help diagnose ED, it is complex and not economical. Therefore, this study aimed to compare NPTR with the IIEF to assess the IIEF's potential as a cost-effective diagnostic tool for ED.</p><p><strong>Methods: </strong>A correlation analysis study was performed on 139 men with ED between August 2017 and March 2023 who had undergone NPTR assessment in Jakarta, Indonesia. ED was assessed using detailed evaluations and IIEF-5 questionnaires. NPTR data was collected using a Rigiscan ^® device. Serum testosterone, total cholesterol, HbA1c, and vitamin D-25(OH) from blood samples were also evaluated. Bivariate analysis was used to explore the correlations between IIEF-5 scores, NPTR measurements, and blood parameters.</p><p><strong>Results: </strong>In total, 139 men with ED (median age: 42 years) were included. The median IIEF-5 score was 11, and comorbidities included dyslipidemia (20%) and diabetes (12%). There are significant correlations between IIEF scores and NPTR variables (number of erections recorded, base tumescence increment, average base tumescence, and duration of base erection with ˃ 60% rigidity). Additionally, significant negative correlations were observed between HbA1c levels and several NPTR variables, including the number and quality of nocturnal erections. Significant correlations were also found between HbA1c and various NPTR variables.</p><p><strong>Conclusion: </strong>This study underscores the value of subjective questionnaires such as the IIEF-5 in diagnosing ED, especially in the absence of advanced tests like the NPTR assessment. We found correlations between IIEF scores and nocturnal erection frequency, as well as specific erection characteristics. Our findings highlight the importance of a personalized approach to ED diagnosis. We also observed that higher HbA1c levels were associated with reduced nocturnal erection quality, highlighting the metabolic influence on erectile function. Although IIEF aids in cost-effective assessments, it should not replace objective testing.</p>","PeriodicalId":12260,"journal":{"name":"F1000Research","volume":"14 ","pages":"142"},"PeriodicalIF":0.0,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12365589/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144947902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Predicting infection with COVID-19 disease using logistic regression model in Karak City, Jordan.","authors":"Anas Khaleel, Wael Abu Dayyih, Lina AlTamimi, Liana Dalaeen, Zainab Zakaraya, Alhareth Ahmad, Baker Albadareen, Abdallah Ahmed Elbakkoush","doi":"10.12688/f1000research.129799.3","DOIUrl":"https://doi.org/10.12688/f1000research.129799.3","url":null,"abstract":"<p><strong>Background: </strong>On March 2020, World Health Organization (WHO) labeled coronavirus disease 2019 (COVID-19) as a pandemic. COVID-19 has rapidly increased in Jordan which resulted in the announcement of the emergency state on March 19th, 2020. Despite the variety of research being reported, there is no agreement on the variables that predict COVID-19 infection. This study aimed to test the predictors that probably contributed to the infection with COVID-19 using a binary logistic regression model.</p><p><strong>Methods: </strong>Based on data collected by Google sheet of COVID-19 infected and non-infected persons in Karak city, analysis was applied to predict COVID-19 infection probability using a binary logistic regression model.</p><p><strong>Results: </strong>A total of 386 participants have completed the questionnaire including 323 women and 63 men. Among the participants 295 (76.4%) were aged less than or equal 45 years old, and 91 (23.6%) were aged over 45 years old. Among the 386 participants a total of 275 were infected with COVID-19. The Logistic regression test was used to analyze every demographic characteristic (sex, age, job, smoking, chronic disease, yearly flu injection) in this study to find predictors of the likelihood of COVID-19 infection. The findings indicate that the participants' sex and age are the most important demographic determinants of infection. Female gender was associated with higher infection risk compared to males (OR = 2.04, 95% CI: 1.17-3.58, p = 0.012). Participants aged >45 years had increased infection risk compared to those ≤45 years (OR = 1.91, 95% CI: 1.11-3.30, p = 0.020). Cox & Snell R Square (R2 = 0.028) and Nagelkerke R Square (R2 = 0.039) indicators were used to measure model fineness with a significant P-value < 0.05.</p><p><strong>Conclusions: </strong>Given a person's age and sex, the final model presented in this study can be used to calculate the probability of infection with COVID-19 in Karak city. This could help aid health-care management and policymakers in properly planning and allocating health-care resources.</p>","PeriodicalId":12260,"journal":{"name":"F1000Research","volume":"12 ","pages":"126"},"PeriodicalIF":0.0,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12374151/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144991870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Marital Bargaining and Assortative Mating on Fertility Preference : Evidence based on Cross-sectional Data in China.","authors":"Meiyi Zhuang, Hisahiro Naito","doi":"10.12688/f1000research.151196.4","DOIUrl":"10.12688/f1000research.151196.4","url":null,"abstract":"<p><strong>Background: </strong>Behaviors regarding child-bearing are among the most consequential ones within families, as child-rearing necessitates the active involvement of both partners. This dynamic suggests that individuals may seek partners with similar fertility preferences, leading to assortative mating based on these shared preferences.</p><p><strong>Data and methods: </strong>This study investigates second-child fertility outcomes using data from the 2018 China Family Panel Studies and applies the Ordinary Least Squares (OLS) regression. Furthermore, it examines assortative matching based on fertility preferences, utilizing the 2020 provincial-level sex ratio for individuals aged 20-39 as a proxy for women's bargaining power in the marriage market.</p><p><strong>Results: </strong>The results, based on a sample of 2,351 household-level observations, indicate that spouses' preferences are complementary. When a husband (or wife) desires a second child, their own preference has a stronger impact on the actual outcome to have of having a second child if their partner also expresses the same desire. Additionally, the findings reveal evidence of assortative mating in terms of preferences for the desired number of children. Moreover, in provinces with higher male-to-female sex ratios-indicating stronger bargaining power for women-a wife's preference is more strongly aligned with her husband's preference. For men, however, a husband's preference aligns with his wife's preference regardless of the sex ratio, suggesting that men's bargaining power in the marriage market is relatively weak.</p><p><strong>Conclusions: </strong>The findings have two key implications. First, shared fertility preferences between spouses show a complementary effect, increasing the likelihood of having a second child. Second, marriage matching is not random, as individuals are more likely to partner with those who share similar fertility preferences. Additionally, women with greater bargaining power are positively associated with their husbands' desired family size.</p>","PeriodicalId":12260,"journal":{"name":"F1000Research","volume":"13 ","pages":"659"},"PeriodicalIF":0.0,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12351236/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144872192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A structured dataset of the federalist society's public engagements.","authors":"Chad M Topaz","doi":"10.12688/f1000research.161735.2","DOIUrl":"10.12688/f1000research.161735.2","url":null,"abstract":"<p><strong>Background: </strong>The Federalist Society, a leading conservative legal organization, has played a significant role in shaping the American judiciary for decades. Despite its influence, comprehensive empirical data on the organization remains scarce. We address this gap by systematically documenting 20,205 public events hosted by the Society from 1984 to 2024, with substantive coverage from 2007 onward.</p><p><strong>Methods: </strong>Following ethical best practices in data collection and ownership, we gathered event metadata-including titles, dates, locations, sponsors, topics, and speakers-via web scraping from the Federalist Society's archives. The dataset is structured to facilitate analysis of event trends, co-speaking networks, and thematic shifts over time. To ensure data integrity, we performed validation, deduplication, and cleaning, resulting in a well-structured, high-quality dataset.</p><p><strong>Conclusions: </strong>This dataset provides an empirical foundation for examining the Federalist Society's role in legal discourse. It is a resource for scholars in law, political science, sociology, gender studies, network science, and computational social science, enabling investigations into key themes of discussion, event participation patterns, demographic diversity, professional backgrounds, and more. Researchers can use it to study institutional influence, legal networks, and the evolution of conservative legal thought.</p>","PeriodicalId":12260,"journal":{"name":"F1000Research","volume":"14 ","pages":"213"},"PeriodicalIF":0.0,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12203041/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144527104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protocol for a co-design study for the development of a chronotherapeutic mobile health behaviour change intervention targeting light exposure among older adults.","authors":"Zahrah Alwi Alkaff, Resshaya Roobini Murukesu, Denz Del Villar, Julia Woerle, Manuel Spitschan","doi":"10.12688/f1000research.157814.2","DOIUrl":"https://doi.org/10.12688/f1000research.157814.2","url":null,"abstract":"<p><p>Through its wide-ranging effects on human physiology and behaviour, daily light exposure is an important environmental modulator of healthy ageing. Integrating mobile health (mHealth) technology with behaviour change strategies offers a promising approach to optimise light exposure and positively impact sleep, rest-wake cycles, cognitive function, and mood at scale. This study aims to develop the LightSPAN mHealth behaviour change intervention to optimise light exposure across the lifespan. Employing a co-design methodology, the study comprises two distinct workstreams. The first focuses on conceptualising the theoretical framework and implementation strategies through a comprehensive review of light exposure interventions, behaviour change theories, mHealth user personas, and recommendations for designing mHealth interventions for older adults. The second workstream centres on co-designing the intervention, involving consultation with community service providers and engagement with older adults at ageing community centres (≥60 years of age). Community service providers will be consulted through a focus group discussion (target n=5). Older adult participants (n=20) will engage in telephone interviews, focus group discussions and prototyping workshops to explore older adult participants' characteristics, needs, preferences, and mHealth intervention design elements and co-design the LightSPAN mHealth behaviour change intervention. The insights generated in these co-design components will facilitate future implementation of our intervention.</p>","PeriodicalId":12260,"journal":{"name":"F1000Research","volume":"13 ","pages":"1356"},"PeriodicalIF":0.0,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12361882/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144948260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Estimation of Covid-19 lungs damage based on computer tomography images analysis.","authors":"Martin Schätz, Olga Rubešová, Jan Mareš, David Girsa, Alan Spark","doi":"10.12688/f1000research.109020.3","DOIUrl":"https://doi.org/10.12688/f1000research.109020.3","url":null,"abstract":"<p><p>Modern treatment is based on reproducible quantitative analysis of available data. The Covid-19 pandemic did accelerate development and research in several multidisciplinary areas. One of them is the use of software tools for faster and reproducible patient data evaluation. A CT scan can be invaluable for a search of details, but it is not always easy to see the big picture in 3D data. Even in the visual analysis of CT slice by slice can inter and intra variability makes a big difference. We present an ImageJ tool developed together with the radiology center of Faculty hospital Královské Vinohrady for CT evaluation of patients with COVID-19. The tool was developed to help estimate the percentage of lungs affected by the infection. The patients can be divided into five groups based on percentage score and proper treatment can be applied.</p>","PeriodicalId":12260,"journal":{"name":"F1000Research","volume":"11 ","pages":"326"},"PeriodicalIF":0.0,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12365592/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144948030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mycotoxin binder for immune and intestinal histopathology ameliorations against Newcastle disease in vaccinated broilers.","authors":"Erma Safitri, Hery Purnobasuki, Tita Damayanti Lestari, Suzanita Utama, Merisa Wahyu Erdhina, Maulida Ilma Sadida, Eka Pramyrtha Hestianah, Mohammad Anam Al Arif, Chairul Anwar Nidom, Sri Mulyati, Jola Rahmahani, Martia Rani Tacharina, Sri Hidanah, Siti Darodjah Rasad, Goo Jang, Mitsuhiro Takagi, Muhammad Thohawi Elziyad Purnama","doi":"10.12688/f1000research.158103.3","DOIUrl":"https://doi.org/10.12688/f1000research.158103.3","url":null,"abstract":"<p><strong>Background: </strong>In broiler farming, vaccination against Newcastle disease (ND) is essential. Nonetheless, during the post-vaccination phase, production may be negatively impacted by mycotoxin contamination in feed. This study aimed to evaluate the effect of mycotoxin binders on immune and intestinal histopathology ameliorations against ND in vaccinated broilers with aflatoxin B1 (AFB1) and ochratoxin A (OTA) toxication.</p><p><strong>Methods: </strong>A total of 20 broilers were randomly assigned into 4 groups with 5 replications i.e. (C-) broiler groups with basal feed, (C+) broiler groups with AFB1 and OTA feed contamination, (T1) and (T2) broiler groups with exposed AFB1, OTA, and toxin binders as feed additives with dose 1.1 g/kg and 1.6 g/kg feed, respectively. ND vaccination was carried out on day 7 and 21. Antibody titers were evaluated from serum samples of broiler on days 14, 28, and 35 for further hemagglutination inhibition (HI) test. Histopathology of the cecum and colon organs was evaluated using HE staining on day 36. HI test and histological scoring were analyzed using the One-Way ANOVA, followed by Duncan's test with a p < 0.05 in SPSS v.26 software (IBM Corp., Armonk, NY).</p><p><strong>Results: </strong>As a result, histopathological improvement of the cecum and colon was reported based on mucosal rupture, hemorrhage and necrosis on day 35. A significant increase in antibody titer was observed on day 35 in the C- and T2 groups, which differed significantly (p < 0.05) from the C+ and T1 groups.</p><p><strong>Conclusions: </strong>This study revealed that a 1.6 g/kg toxin binder dose in feed can increase antibody titer and histopathology of cecum and colon in broiler chickens after ND vaccination fed with mycotoxin-contaminated feed.</p>","PeriodicalId":12260,"journal":{"name":"F1000Research","volume":"13 ","pages":"1518"},"PeriodicalIF":0.0,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12361880/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144948231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}