F1000Research最新文献

{"title":"The effects of multifactorial pharmacist-led intervention protocol on medication optimisation and adherence among patients with type 2 diabetes: A randomised control trial.","authors":"Marwan El-Deyarbi, Luai Ahmed, Jeffrey King, Syed Abubackar, Ahmed Al Juboori, Nirmin A Mansour, Salahdein Aburuz","doi":"10.12688/f1000research.146517.2","DOIUrl":"10.12688/f1000research.146517.2","url":null,"abstract":"<p><strong>Background: </strong>Patient-related factors and limited medication adherence in patients with chronic diseases, are associated with poor clinical outcomes, long-term complications, and increased overall disease costs. Many methods have been tested with mixed results, and innovative approaches are needed to encourage patients to adhere to their prescribed drug regimens.</p><p><strong>Methods: </strong>This randomised controlled trial examined a new multifactorial pharmacist-led intervention protocol (MPIP), including a medication therapy management (MTM) program with face-to-face counselling, patient-specific medication booklets, and a mobile application, from July 2021 to September 2022 in the Oud Al Touba diagnostic and screening ambulatory centre in 192 patients with type 2 diabetes in the United Arab Emirates. Medication adherence was assessed using the fixed medication possession ratio of medication refills and the medication adherence questionnaire.</p><p><strong>Results: </strong>At 12 months follow-up, participants in the MPIP showed significant improvement in overall medication adherence with total (composite) medication possession ratio (MPRt) of mean (±SD) 0.95 (±0.09) compared to 0.92 (± 0.09) in the control group with mean difference of 0.03 (95%, CI 0.01-0.06), P =0.02. In addition, improvement trend was evident in the MPIP group for all medication regimens with P value <0.01. Comparable results were noticeable in adherence questionnaire scores at the end of the study, with 66 participants in the intervention group scored zero on the questionnaire, suggesting high adherence to medication compared to the control group (48 participants only). The MTM program performed 41 clinical interventions on drug-related problems, compared to six interventions in the control group, and the use of mobile application and medication booklet have increased to 45.7% compared to 21.4% before study exit.</p><p><strong>Conclusions: </strong>The pharmacy intervention protocol effectively improved medication adherence and optimised medication regimens in diabetic patients with chronic medication regimens in an ambulatory healthcare centre.</p>","PeriodicalId":12260,"journal":{"name":"F1000Research","volume":"13 ","pages":"493"},"PeriodicalIF":0.0,"publicationDate":"2024-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11421608/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142344445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Psychological interventions for early-phase schizophrenia: protocol for a systematic review and network meta-analysis.","authors":"Lena Feber, Georgia Salanti, Mathias Harrer, Nurul Husna Salahuddin, Wulf-Peter Hansen, Josef Priller, Irene Bighelli, Stefan Leucht","doi":"10.12688/f1000research.152191.1","DOIUrl":"10.12688/f1000research.152191.1","url":null,"abstract":"<p><strong>Introduction: </strong>Treating the early phase of schizophrenia is crucial for preventing further episodes and improving quality of life, functioning, and social inclusion. Pharmacotherapies are first-line treatments, but have limitations. There is consensus on the need for non-pharmacological interventions for individuals in the early phase of schizophrenia. Several psychological interventions have shown promising effects; however, their comparative effectiveness remains largely unknown. To address this issue, a network meta-analysis will be performed. We aim to develop a hierarchy of existing psychological treatments concerning their efficacy and tolerability, which will inform treatment guidelines.</p><p><strong>Protocol: </strong>Randomized controlled trials (RCTs) investigating psychological interventions for first-episode psychosis, first-episode schizophrenia, or early phase schizophrenia will be included. The primary outcome will be overall schizophrenia symptoms (measured up to 6 and 12 months, and at the longest follow-up) and relapse as a co-primary outcome. Secondary outcomes are premature discontinuation; change in positive, negative, and depressive symptoms of schizophrenia; response; quality of life; overall functioning; satisfaction with care; adherence; adverse events; and mortality. The study selection and data extraction are performed by two independent reviewers. We will assess the risk of bias of each study using the Cochrane Risk of Bias tool 2 and evaluate the confidence in the results using Confidence in Network Meta-Analysis (CINeMA). Subgroup and sensitivity analyses will be conducted to explore heterogeneity and assess the robustness of our findings.</p><p><strong>Discussion: </strong>This systematic review and network meta-analysis aims to compare multiple existing psychological interventions, establishing which are best for symptom reduction, relapse prevention, and other important outcomes in early phase schizophrenia. Our results may provide practical guidance concerning the most effective psychological intervention to reduce symptom severity and the societal burden associated with the disorder.</p>","PeriodicalId":12260,"journal":{"name":"F1000Research","volume":"13 ","pages":"649"},"PeriodicalIF":0.0,"publicationDate":"2024-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11375411/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142139698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of a low, medium, and high-intensity aquatic physiotherapy protocol on functional and biochemical parameters in individuals with knee osteoarthritis: protocol for a crossover randomized controlled trial.","authors":"Cesar Augusto Teixeira, Lisete Haas, Bruna Frata, Andressa Fiori Bortoli, Fernanda Scalco Acco, Gustavo de Castro, Fernanda Cechetti","doi":"10.12688/f1000research.140342.4","DOIUrl":"10.12688/f1000research.140342.4","url":null,"abstract":"<p><strong>Background: </strong>Knee osteoarthritis affects the performance of daily activities, independence, and quality of life. The etiopathogenesis of this condition considers the mechanisms of activation of metalloproteinase and reactive oxygen species production pathways. Metalloproteinases-3 (MMP-3) and Glutathione Peroxidase (GPx) may be responsible for cartilage destruction. Aquatic physiotherapy promotes a positive impact on the clinical picture of osteoarthritis, and this study presents an intervention protocol that aims to evaluate the effects of a single session of different aquatic physiotherapy modalities on the biochemical and functional behavior of patients with knee osteoarthritis.</p><p><strong>Methods: </strong>This will be a crossover randomized controlled trial in which 15 individuals will be submitted to three aquatic physiotherapy modalities with a minimum 15-day wash-out period in patients over 50 years old and diagnosed with OA in at least one knee, presence of pain and at least one functional dysfunction for at least 6 months, absence of physical limitation that prevents the exercise protocol from being performed, Kellgren and Lawrence ranking between I and IV, walk independently and without auxiliary device. Variations in the concentrations of MMP-3 and GPx in peripheral blood, pain, edema, and flexibility resulting from the three aquatic physiotherapeutic interventions will be evaluated both pre- and immediate post-intervention. The reference group will be submitted to the same aquatic physiotherapy protocols, however, only the biochemical parameters and the self-perception questionnaires will be evaluated.</p><p><strong>Registration: </strong>ClinicalTrials.gov ( NCT05610696, 18/01/2023).</p>","PeriodicalId":12260,"journal":{"name":"F1000Research","volume":"12 ","pages":"1605"},"PeriodicalIF":0.0,"publicationDate":"2024-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11409911/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142282742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analyzing Molecular Traits of H9N2 Avian Influenza Virus Isolated from a Same Poultry Farm in West Java Province, Indonesia, in 2017 and 2023.","authors":"Muhammad Ade Putra, Amin Soebandrio, I Wayan Teguh Wibawan, Christian Marco Hadi Nugroho Nugroho, Ryan Septa Kurnia, Otto Sahat Martua Silaen, Rifky Rizkiantino, Agustin Indrawati, Okti Nadia Poetri, Desak Gede Budi Krisnamurti","doi":"10.12688/f1000research.150975.2","DOIUrl":"10.12688/f1000research.150975.2","url":null,"abstract":"<p><strong>Background: </strong>Indonesia is one of the countries that is endemic to avian influenza virus subtype H9N2. This study aims to compare the molecular characteristics of avian influenza virus (AIV) subtype H9N2 from West Java.</p><p><strong>Methods: </strong>Specific pathogen-free (SPF) embryonated chicken eggs were used to inoculate samples. RNA extraction and RT-qPCR confirmed the presence of H9 and N2 genes in the samples. RT-PCR was employed to amplify the H9N2-positive sample. Nucleotide sequences were obtained through Sanger sequencing and analyzed using MEGA 7. Homology comparison and phylogenetic tree analysis, utilizing the neighbor-joining tree method, assessed the recent isolate's similarity to reference isolates from GenBank. Molecular docking analysis was performed on the HA1 protein of the recent isolate and the A/Layer/Indonesia/WestJava-04/2017 isolate, comparing their interactions with the sialic acids Neu5Ac2-3Gal and Neu5Ac2-6Gal.</p><p><strong>Results: </strong>RT-qPCR confirmed the isolate samples as AIV subtype H9N2. The recent virus exhibited 11 amino acid residue differences compared to the A/Layer/Indonesia/WestJava-04/2017 isolate. Phylogenetically, the recent virus remains within the h9.4.2.5 subclade. Notably, at antigenic site II, the recent isolate featured an amino acid N at position 183, unlike A/Layer/Indonesia/WestJava-04/2017. Molecular docking analysis revealed a preference of HA1 from the 2017 virus for Neu5Ac2-3Gal, while the 2023 virus displayed a tendency to predominantly bind with Neu5Ac2-6Gal.</p><p><strong>Conclusion: </strong>In summary, the recent isolate displayed multiple mutations and a strong affinity for Neu5Ac2-6Gal, commonly found in mammals.</p>","PeriodicalId":12260,"journal":{"name":"F1000Research","volume":"13 ","pages":"571"},"PeriodicalIF":0.0,"publicationDate":"2024-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11602698/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142750508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An enhanced broadband class-J mode power amplifier for 5G smart meter applications.","authors":"Nagisetty Sridhar, Dr Chinnaiyan Senthilpari, Dr Mardeni R, Dr Wong Hin Yong","doi":"10.12688/f1000research.73498.2","DOIUrl":"https://doi.org/10.12688/f1000research.73498.2","url":null,"abstract":"<p><p><b>Background:</b> With the tremendous increase in the usage of smart meters for industrial/ household purposes, their implementation is considered a crucial challenge in the Internet of Things (IoT) world, leading to a demand for emerging 5G technology. In addition, a large amount of data has to be communicated by smart meters efficiently, which needs a significant enhancement in bandwidth. The power amplifier (PA) plays a major role in deciding the efficiency and bandwidth of the entire communication system. Among the various modes of PAs, a newly developed Class-J mode PA has been proven to achieve high efficiency over a wide bandwidth by maintaining linearity. <b>Methods:</b> This paper proposes a Class-J mode PA design methodology using a CGH40010F-GaN device that operates at a 3.5 GHz frequency to meet the requirements of 5G wireless communication technology for the replacement of existing 4G/LTE technology used for advanced metering infrastructure (AMI) in smart grids. This research's main objective is to design the proper matching networks (M.Ns) to achieve Class-J mode operation that satisfies the bandwidth requirements of 5G smart grid applications. With the target impedances obtained using the load-pull simulation, lumped element matching networks are analyzed and designed in 3 ways using the ADS EDA tool. <b>Results:</b> The simulation results reveal that the proposed Class-J PA provides a maximum drain efficiency (D.E) of 82%, power added efficiency (PAE) of 67% with 13 dB small-signal gain at 3.5 GHz, and output power of 40 dBm (41.4 dBm peak) with a power gain of approximately 7 dB over a bandwidth of approximately 400 MHz with a 28 V power supply into a 50 Ω load. <b>Conclusion:</b> The efficiency and bandwidth of the proposed Class-J PA can be enhanced further by fine-tuning the matching network design to make it more suitable for 5G smart meter/grid applications.</p>","PeriodicalId":12260,"journal":{"name":"F1000Research","volume":"10 ","pages":"1099"},"PeriodicalIF":0.0,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11523559/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142544576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of histopathological and triphenyl tetrazolium chloride test in diagnosing myocardial infarction: An autopsy study.","authors":"Varun Krishna B, Chandni Gupta, Vikram Palimar, Anitha S, Deepak Nayak M","doi":"10.12688/f1000research.152421.1","DOIUrl":"https://doi.org/10.12688/f1000research.152421.1","url":null,"abstract":"<p><strong>Background: </strong>Sudden and unexpected deaths are increasing drastically. The main cause of sudden death is cardiovascular disease, out of which coronary artery disease predominates forming 80% of the cases. Most of the time, detecting early changes in myocardial infarction during the autopsy is challenging since gross infarct changes do not appear until after 24 to 48 hours of myocardial ischemia injury. So, the aim of this study was to compare two test to detect early changes of Myocardial Infarction one by using Triphenyl Tetrazolium Chloride (TTC) staining of the myocardial tissue, during autopsy and other by histopathological examination.</p><p><strong>Methods: </strong>The sample size of 60 hearts taken from all the sudden deaths cases brought to Mortuary with suspected cause of death due to cardiac origin. The heart was obtained from the deceased by standard post-mortem technique. Serial full-thickness transverse sections of the heart were taken at 2 cm intervals from the apex to the atrioventricular groove. All the serial slices of heart are taken for histochemical staining and TTC staining.</p><p><strong>Results: </strong>In histopathological examination 34 hearts were diagnosed with myocardial infarction and 26 hearts reported non myocardial infarction. With TTC 40 hearts remained unstained suggestive of myocardial infarction and 20 hearts were stained suggestive of non-infarcted hearts. TTC staining in our study shows an accuracy of 88.33%.</p><p><strong>Conclusion: </strong>The result of this study shows that the Triphenyl Tetrazolium Chloride test, a histochemical staining technique of heart, is reliable approach for forensic pathologists to diagnose early myocardial infarction during the post-mortem examination.</p>","PeriodicalId":12260,"journal":{"name":"F1000Research","volume":"13 ","pages":"1050"},"PeriodicalIF":0.0,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11411240/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142282754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In silico analysis of molecular mimicry between human aquaporin 3, <i>Aspergillus fumigatus</i> aquaporin and aquaporins from allergic sources.","authors":"Andrés Sánchez, Yaquelin Padilla, Adriana Lorduy, Jorge Sanchez, Marlon Munera, Claudia Baena, Carlos Bernal, Juan Urrego","doi":"10.12688/f1000research.142843.2","DOIUrl":"10.12688/f1000research.142843.2","url":null,"abstract":"<p><strong>Background: </strong>Atopic dermatitis (AD) is a chronic inflammatory skin condition that has a significant impact on quality of life. The immune response and allergy symptoms in AD are triggered by the recognition of specific allergens by IgE antibodies. Cross-reactivity can lead to auto-IgE responses, potentially worsening AD symptoms. Our research aimed to enhance our understanding of allergenic sources, including A. fumigatus, and their role in AD. We focused on molecular mimicry between human AQP3 and A. fumigatus aquaporin.</p><p><strong>Methods: </strong>In our in-silico analysis, we compared the amino acid sequences of human aquaporin 3 (AQP3) and A. fumigatus aquaporin with 25 aquaporins from various allergenic sources, sourced from the UniProt and NCBI databases. Phylogenetic relationship analysis and homology-based modeling were conducted. We identified conserved antigenic regions located within the 3D structures.</p><p><strong>Results: </strong>The global identity levels among the studied aquaporins averaged 32.6%. One antigenic site exhibited a remarkable local region, with a conserved identity of 71.4%. We categorized the aquaporins into five monophyletic clades (A-E), with group B showing the highest identity (95%), including six mammalian aquaporins, including AQP3. When comparing <i>A. fumigatus</i> aquaporins, the highest identity was observed with <i>Malassezia sympodialis</i> at 35%. Both human and A. fumigatus aquaporins have three linear and three discontinuous epitopes.</p><p><strong>Conclusions: </strong>We identified potential linear and conformational epitopes of AQP3, indicating a possible molecular mimicry between humans and <i>A. fumigatus</i> aquaporins. This suggests autoreactivity and potential cross-reactivity, although further validation using in vitro and in vivo experiments is required.</p>","PeriodicalId":12260,"journal":{"name":"F1000Research","volume":"13 ","pages":"358"},"PeriodicalIF":0.0,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11415755/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142282759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The frailty among suburban elderly population after one-year COVID-19 pandemic in Cirebon Regency, Indonesia.","authors":"Ahmad Fariz Malvi Zamzam Zein, Witri Pratiwi, Naswidi Dohana","doi":"10.12688/f1000research.145504.2","DOIUrl":"10.12688/f1000research.145504.2","url":null,"abstract":"<p><strong>Background: </strong>The coronavirus disease 2019 (COVID-19) pandemic has had significant impacts worldwide, especially among older adults. Frailty is a determinant of susceptibility to morbidity and mortality due to COVID-19 in the elderly. This study aimed to determine frailty status and identify factors associated with the suburban elderly population in Cirebon Regency, Indonesia, after the one-year COVID-19 pandemic.</p><p><strong>Methods: </strong>A cross-sectional study of community-dwelling individuals aged ≥ 60 years was conducted in Klangenan, Cirebon Regency, Indonesia, from March to June 2021. A questionnaire was used to determine the baseline characteristics of participants, healthcare access, comorbidity, and frailty status. The Ina-FRAIL scale was used to determine the frailty status (frail/non-frail). The chi-square test and logistic regression analysis were used to determine the association between independent variables and frailty.</p><p><strong>Results: </strong>A total of 383 participants were recruited, with a median age of 67 (IQR 64-73) years. The prevalence of frailty in the present study was 10.2%. Multivariate analysis showed that age (OR 2.73; 95%CI 1.21-6.12), multimorbidity (OR 7.86; 95% CI 3.01-20.57) and financial dependence (OR 13.40, 95% CI 5.66-31.73) were significantly associated with frailty.</p><p><strong>Conclusion: </strong>One-year COVID-19 pandemic has had a considerable burden on frailty among the suburban elderly population in Indonesia. The factors associated with frailty were age, multimorbidity, and financial dependence.</p>","PeriodicalId":12260,"journal":{"name":"F1000Research","volume":"13 ","pages":"151"},"PeriodicalIF":0.0,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11342023/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142055327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of thermomechanical parameters on surface texture in filament materials extrusion: outlook and trends.","authors":"John D Kechagias","doi":"10.12688/f1000research.144965.1","DOIUrl":"https://doi.org/10.12688/f1000research.144965.1","url":null,"abstract":"<p><p>The material extrusion process has been widely used to manufacture custom products. However, the surface texture varies due to the additive mechanism of the process, which depends on the layer height and surface orientation, resulting in varying average surface roughness values for inclined, flat and vertical surfaces. Different strand welding conditions result in non-uniform internal stresses, surface distortions, layer traces, weak bonding, non-uniform pores and material overflow. This paper comprehensively examines material extrusion process achievements in surface texture quality and studies and summarises the most influential processing parameters. Parameter effects are critically discussed for each topic; flat, inclined, and vertical surfaces. The results of this research help reduce post-processing.</p>","PeriodicalId":12260,"journal":{"name":"F1000Research","volume":"13 ","pages":"1039"},"PeriodicalIF":0.0,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11541081/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142603705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessing the cytotoxicity of phenolic and terpene fractions extracted from Iraqi <i>Prunus arabica</i> against AMJ13 and SK-GT-4 human cancer cell lines.","authors":"Matin Adil Mahmood, Abdulkareem Hameed Abd, Enas Jawad Kadhim","doi":"10.12688/f1000research.131336.3","DOIUrl":"https://doi.org/10.12688/f1000research.131336.3","url":null,"abstract":"<p><p><b>Background:</b> Breast and esophageal cancer are the most aggressive and prominent causes of death worldwide. In addition, these cancers showed resistance to current chemotherapy regimens with limited success rates and fatal outcomes. Recently many studies reported the significant cytotoxic effects of phenolic and terpene fractions extracted from various <i>Prunus</i> species against different cancer cell lines. As a result, it has a good chance to be tested as a complement or replacement for standard chemotherapies. <b>Methods:</b> The study aimed to evaluate the cytotoxicity of phenolic and terpene fractions extracted from Iraqi <i>Prunus arabica</i> on breast (AMJ13) and esophageal (SK-GT-4) cancer cell lines by using the MTT assay (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide). Analysis using the Chou-Talalay method was performed to assess the synergistic effect between the extracted fractions and chemotherapeutic agent (docetaxel). Moreover, high-performance liquid chromatography (HPLC) analysis was conducted for the quantitative determination of different bioactive molecules of both phenolic and terpene fractions in the extract. <b>Results:</b> According to the findings, the treatment modalities significantly decreased cancer cell viability of AMJ13 and SK-GT-4 and had insignificant cytotoxicity on the normal cells (normal human fibroblast cell line) (all less than 50% cytotoxicity). Analysis with Chou-Talalay showed a strong synergism with docetaxel on both cancer cell lines (higher cytotoxicity even in low concentrations) and failed to induce cytotoxicity on the normal cells. Important flavonoid glycosides and terpenoids were detected by HPLC, in particularly, ferulic acid, catechin, chlorogenic acid, β-sitosterol, and campesterol. <b>Conclusions:</b> In conclusion, the extracted fractions selectively inhibited the proliferation of both cancer cell lines and showed minimal cytotoxicity on normal cells. These fractions could be naturally derived drugs for treating breast and esophageal cancers.</p>","PeriodicalId":12260,"journal":{"name":"F1000Research","volume":"12 ","pages":"433"},"PeriodicalIF":0.0,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11480737/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142461500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}