EXCLI Journal最新文献

{"title":"mRNA-engineered T cells against telomerase: a novel immunotherapy approach for cancer.","authors":"Mudasir Maqbool, Zulfkar Qadrie, Amita Joshi Rana, Sumel Ashique, Md Sadique Hussain","doi":"10.17179/2025-8327","DOIUrl":"10.17179/2025-8327","url":null,"abstract":"","PeriodicalId":12247,"journal":{"name":"EXCLI Journal","volume":"24 ","pages":"570-572"},"PeriodicalIF":3.8,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12171005/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144316268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Epitope imprinted polymers: a versatile and cost-effective alternative for targeted therapies.","authors":"Liming Zhang, Md Sadique Hussain, Mudasir Maqbool, Sumel Ashique, Gyas Khan","doi":"10.17179/excli2025-8306","DOIUrl":"10.17179/excli2025-8306","url":null,"abstract":"","PeriodicalId":12247,"journal":{"name":"EXCLI Journal","volume":"24 ","pages":"564-566"},"PeriodicalIF":3.8,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12127518/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144208079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Halitosis: the unique scent of colorectal cancer.","authors":"Ying Chen, Xiao Xian Qian","doi":"10.17179/2025-8338","DOIUrl":"10.17179/2025-8338","url":null,"abstract":"","PeriodicalId":12247,"journal":{"name":"EXCLI Journal","volume":"24 ","pages":"567-569"},"PeriodicalIF":3.8,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12171006/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144316266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comment to \"The beneficial effect of combination therapy with sulfasalazine and valsartan in the treatment of ulcerative colitis [EXCLI Journal 2021;20:236-247]\".","authors":"Jan G Hengstler, Agapios Sachinidis","doi":"10.17179/excli2025-8388","DOIUrl":"https://doi.org/10.17179/excli2025-8388","url":null,"abstract":"","PeriodicalId":12247,"journal":{"name":"EXCLI Journal","volume":"24 ","pages":"562-563"},"PeriodicalIF":3.8,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12127517/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144208078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lack of association between atopic dermatitis and COVID-19 severity: results from a case-control study.","authors":"Martha Débora Lira Tenório, Pedro Dantas Oliveira, Paulo Ricardo Martins-Filho","doi":"10.17179/excli2025-8197","DOIUrl":"10.17179/excli2025-8197","url":null,"abstract":"","PeriodicalId":12247,"journal":{"name":"EXCLI Journal","volume":"24 ","pages":"558-561"},"PeriodicalIF":3.8,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12127519/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144208080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Baccharis articulata aqueous extract exerts in vitro antifibrotic effect in hepatic stellate cells by attenuating collagen deposition and TGF-ß1 protein expression.","authors":"Daiana Daniele Boeff, Markus Berger, Mariana Koetz, Pamela Zanon, Alícia da Costa Pereira, Katyuce de Souza Farias, Carlos Alexandre Carollo, Paula Barros Terraciano, Eduardo Luis Konrath","doi":"10.17179/excli2025-8394","DOIUrl":"10.17179/excli2025-8394","url":null,"abstract":"<p><p><i>Baccharis articulata</i> (Lam) Pers. is an herb native to southern Brazil and is widely used in local traditional medicine for weight loss and for the treatment of digestive and liver diseases. However, only a few studies have been conducted to scientifically validate the folk use of this plant. This study assessed the <i>in vitro</i> therapeutic effects of an aqueous extract of <i>B. articulata</i> and chlorogenic acid on liver fibrosis in murine hepatic stellate cells (HSC; GRX cell line). The decrease in cell proliferation and cytotoxicity, as well as phenotypic reversion by the presence of lipid droplets and reduction in collagen content after seven days of treatment, were evaluated. The mechanisms responsible for the antifibrotic effects of the extract, including the plasminogen activation system, were assessed. from high-performance liquid chromatography coupled with diode array detector and tandem mass spectrometry (HPLC-DAD-MS/MS) data. Twenty-six metabolites were identified in the extract, including flavonoids, phenylpropanoid derivatives, and diterpenes. Treatment with the extract significantly induced the accumulation of lipids in the cytoplasm of cells, indicating that it could revert the HSC phenotype to a quiescent state with no cytotoxic or antiproliferative effects. These findings may be related to the inhibition of the TGF-β1 pathway, a biomarker of liver fibrosis, upregulation of the plasminogen system, and dose-dependent inhibition of plasmin activity. The presence of caffeoylquinic acids seems to be partially related to the extract effect, as chlorogenic acid displayed antiproliferative activity and reduced collagen content in hepatic stellate cells. Considering the unmet need for antifibrotic therapies, the use of medicinal plants to inhibit the proliferation of activated HSC is promising, and this study indicated that the aqueous extract of <i>B. articulata</i> has potential therapeutic activity against hepatic fibrosis (see also Figure 1(Fig. 1) graphical abstract).</p>","PeriodicalId":12247,"journal":{"name":"EXCLI Journal","volume":"24 ","pages":"659-676"},"PeriodicalIF":3.8,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12235268/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144590809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of sulforaphane on autism spectrum disorder: systematic review and meta-analysis.","authors":"Rui Wang, Zhenhui Ren, Yamin Li","doi":"10.17179/excli2025-8239","DOIUrl":"10.17179/excli2025-8239","url":null,"abstract":"<p><p>Autism spectrum disorder (ASD) is a complex neurodevelopmental disorder lacking effective treatments. This systematic review and meta-analysis assesses the efficacy and safety of sulforaphane (SFN) for ASD. Eight databases were searched from inception to September 2024, identifying six randomized controlled trials for inclusion. Efficacy outcomes included ASD symptoms measured by the mean difference (MD) or standardized mean difference (SMD), while safety outcomes included adverse events measured by relative risk. Risk of bias was assessed using the Cochrane tool, and evidence certainty was evaluated via the Grade of Recommendations Assessment Development and Evaluation (GRADE). Results showed that SFN significantly improved total symptoms (SMD = -0.27, 95 % confidence interval (CI), -0.42, -0.12), aberrant behavior (SMD = -0.43, 95 % CI, -0.66, -0.19), hyperactivity (SMD = -0.58, 95 % CI, -1.03, -0.13), social interaction (SMD = -0.43, 95 % CI, -0.59, -0.27), social communication (SMD = -0.24, 95 % CI, -0.35, - 0.12), and restricted and repetitive behaviors (RRB) (SMD = -0.16, 95 % CI, -0.31, -0.00). Effects on irritability, anxiety, sensory sensitivity, total social skills, social awareness, social cognition, and social motivation were not statistically significant. Adverse events were similar between intervention and control groups. In conclusion, SFN shows potential in improving ASD symptoms without significant adverse effects. However, results should be interpreted cautiously due to potential influences from assessment tools, outcome assessors, and treatment duration. Further research is needed to confirm the long-term efficacy and safety of SFN for ASD.</p>","PeriodicalId":12247,"journal":{"name":"EXCLI Journal","volume":"24 ","pages":"542-557"},"PeriodicalIF":3.8,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12127520/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144208081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of immune dysregulation in the pathogenesis of type 1 diabetes: a paradigm shift in prevention strategies.","authors":"Rajiv G Gopalsamy, Hariharan Govindasamy, Jessiane B de Souza, Deise M R R Silva, Pedro H M Moura, Ronaldy S Santos, Marina Dos S Barreto, Adriana G Guimarães, Lucas A da M Santana, Lysandro P Borges, Eloia E D Silva","doi":"10.17179/excli2025-8233","DOIUrl":"https://doi.org/10.17179/excli2025-8233","url":null,"abstract":"","PeriodicalId":12247,"journal":{"name":"EXCLI Journal","volume":"24 ","pages":"539-541"},"PeriodicalIF":3.8,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12078776/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144076981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesis, structural studies, and inhibitory potential of selected sulfonamide analogues: insights from in silico and in vitro analyses.","authors":"Tahira Noor, Daniel C Schultz, Gustavo Seabra, Yuting Zhai, Kwangcheol Casey Jeong, Saleem Ahmed Bokhari, Fahim Ashraf Qureshi, Abdul Rauf Siddiqi, Chenglong Li","doi":"10.17179/excli2024-8118","DOIUrl":"https://doi.org/10.17179/excli2024-8118","url":null,"abstract":"<p><p>Antimicrobial resistance is a growing public health threat worldwide, and the current drug development pipeline has thus far been inadequate in addressing this impending crisis. Further research into antibiotic agents, both existing and novel, is therefore paramount for identifying suitable candidates to combat antibiotic-resistant pathogens. Sulfonamides, the first class of synthetic antibiotics, target dihydropteroate synthase (DHPS), a key bacterial enzyme. While this class of antibiotics has historically demonstrated great utility, their use has diminished due to resistance and undesired side effects. In the present study, we synthesized a selection of four sulfonamide analogues (<b>FQ5</b>, <b>FQ6</b>, <b>FQ7</b> and <b>FQ12</b>), validated their structures through NMR spectroscopy, and evaluated their inhibitory potential through computational docking and MIC assays against four bacterial strains: <i>Staphylococcus aureus</i> ATCC 25923, <i>Pseudomonas aeruginosa</i> ATCC 27853, <i>Escherichia coli</i> ATCC 35401 and <i>Bacillus subtilis</i> ATCC 6633. Each compound exhibited antibacterial activity; <b>FQ5</b> demonstrated the most potent activity, with an MIC of 32, 16, 16, and 16 µg/mL against aforementioned strains, respectively. <b>FQ6</b>, <b>FQ7</b> and <b>FQ12</b>, on the other hand, exhibited moderate activity against <i>P. aeruginosa</i> and <i>E. coli</i> (MIC = 128 µg/mL each) and low activity against <i>S. aureus</i> and <i>B. subtilis</i> (MIC = 256 µg/mL each). Molecular docking studies indicated that <b>FQ5</b> captures multiple hydrogen bonding, ionic, and π-π interactions with key binding pocket residues of DHPS, and <b>FQ5</b> also demonstrated superior predicted drug-likeness in <i>in silico</i> ADMET studies compared to other compounds. <b>FQ5</b> is therefore a favorable starting point for further optimization.</p>","PeriodicalId":12247,"journal":{"name":"EXCLI Journal","volume":"24 ","pages":"527-538"},"PeriodicalIF":3.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12078777/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144076979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bacteriophages as potential therapeutic agents in the control of bacterial infections.","authors":"Felipe Gomes Dallepiane, Malena Alejandro Coimbra Nogueira, Lucas Menezes Dos Anjos, Gilberto De Souza Melo, João Paulo De Carli, Bruno Henriques, Gislaine Fongaro, Ariadne Cristiane Cabral Cruz","doi":"10.17179/excli2025-8145","DOIUrl":"https://doi.org/10.17179/excli2025-8145","url":null,"abstract":"","PeriodicalId":12247,"journal":{"name":"EXCLI Journal","volume":"24 ","pages":"524-526"},"PeriodicalIF":3.8,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12078781/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144076928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}