EXCLI Journal最新文献

{"title":"m6A methylation: a new frontier in epilepsy research and therapeutics.","authors":"Mudasir Maqbool, Yumna Khan, Mohammed M Arab, Saud O Alshammari, Md Sadique Hussain, Fawaz M Almufarriji","doi":"10.17179/2025-8359","DOIUrl":"10.17179/2025-8359","url":null,"abstract":"<p><p>Epilepsy is a highly complex and global neurological disorder, for which available treatments only inadequately control the disease in many patients. Recent advances in molecular research have identified N6-methyladenosine (m6A) RNA modifications as key regulators of neuronal processes that underpin the pathophysiology of epilepsy. This review critically discusses the emerging significance of m6A modifications in epilepsy, focusing on dynamic regulations of m6A \"writers,\" \"erasers,\" and \"readers\" for modulating gene expression, neuronal excitability, and synaptic plasticity in epilepsy. Dysregulation of m6A machinery promotes epilepsy by exacerbating oxidative stress, mitochondrial dysfunction, and neuronal damage. We also discuss the prognostic significance of m6A alterations as a potential biomarker in epilepsy diagnosis and disease progression, along with advanced therapeutic strategies against m6A, including small molecules, RNA editing technologies, and precision medicine. This review highlights the transformational significance of m6A modulation in epilepsy therapy and opens new avenues for personalized therapeutic strategies that may revolutionize the field of drug-resistant epilepsy and improve the prognosis for patients. See also the graphical abstract(Fig. 1).</p>","PeriodicalId":12247,"journal":{"name":"EXCLI Journal","volume":"24 ","pages":"578-611"},"PeriodicalIF":3.8,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12171009/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144316267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Twenty years of inhaled insulin: promise, setbacks, and future directions.","authors":"Meriem Gaddas, Imen Ben Saida, Helmi Ben Saad","doi":"10.17179/2025-8260","DOIUrl":"10.17179/2025-8260","url":null,"abstract":"","PeriodicalId":12247,"journal":{"name":"EXCLI Journal","volume":"24 ","pages":"573-577"},"PeriodicalIF":3.8,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12171007/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144316269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"mRNA-engineered T cells against telomerase: a novel immunotherapy approach for cancer.","authors":"Mudasir Maqbool, Zulfkar Qadrie, Amita Joshi Rana, Sumel Ashique, Md Sadique Hussain","doi":"10.17179/2025-8327","DOIUrl":"10.17179/2025-8327","url":null,"abstract":"","PeriodicalId":12247,"journal":{"name":"EXCLI Journal","volume":"24 ","pages":"570-572"},"PeriodicalIF":3.8,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12171005/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144316268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Epitope imprinted polymers: a versatile and cost-effective alternative for targeted therapies.","authors":"Liming Zhang, Md Sadique Hussain, Mudasir Maqbool, Sumel Ashique, Gyas Khan","doi":"10.17179/excli2025-8306","DOIUrl":"10.17179/excli2025-8306","url":null,"abstract":"","PeriodicalId":12247,"journal":{"name":"EXCLI Journal","volume":"24 ","pages":"564-566"},"PeriodicalIF":3.8,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12127518/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144208079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Halitosis: the unique scent of colorectal cancer.","authors":"Ying Chen, Xiao Xian Qian","doi":"10.17179/2025-8338","DOIUrl":"10.17179/2025-8338","url":null,"abstract":"","PeriodicalId":12247,"journal":{"name":"EXCLI Journal","volume":"24 ","pages":"567-569"},"PeriodicalIF":3.8,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12171006/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144316266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comment to \"The beneficial effect of combination therapy with sulfasalazine and valsartan in the treatment of ulcerative colitis [EXCLI Journal 2021;20:236-247]\".","authors":"Jan G Hengstler, Agapios Sachinidis","doi":"10.17179/excli2025-8388","DOIUrl":"https://doi.org/10.17179/excli2025-8388","url":null,"abstract":"","PeriodicalId":12247,"journal":{"name":"EXCLI Journal","volume":"24 ","pages":"562-563"},"PeriodicalIF":3.8,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12127517/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144208078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lack of association between atopic dermatitis and COVID-19 severity: results from a case-control study.","authors":"Martha Débora Lira Tenório, Pedro Dantas Oliveira, Paulo Ricardo Martins-Filho","doi":"10.17179/excli2025-8197","DOIUrl":"10.17179/excli2025-8197","url":null,"abstract":"","PeriodicalId":12247,"journal":{"name":"EXCLI Journal","volume":"24 ","pages":"558-561"},"PeriodicalIF":3.8,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12127519/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144208080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of sulforaphane on autism spectrum disorder: systematic review and meta-analysis.","authors":"Rui Wang, Zhenhui Ren, Yamin Li","doi":"10.17179/excli2025-8239","DOIUrl":"10.17179/excli2025-8239","url":null,"abstract":"<p><p>Autism spectrum disorder (ASD) is a complex neurodevelopmental disorder lacking effective treatments. This systematic review and meta-analysis assesses the efficacy and safety of sulforaphane (SFN) for ASD. Eight databases were searched from inception to September 2024, identifying six randomized controlled trials for inclusion. Efficacy outcomes included ASD symptoms measured by the mean difference (MD) or standardized mean difference (SMD), while safety outcomes included adverse events measured by relative risk. Risk of bias was assessed using the Cochrane tool, and evidence certainty was evaluated via the Grade of Recommendations Assessment Development and Evaluation (GRADE). Results showed that SFN significantly improved total symptoms (SMD = -0.27, 95 % confidence interval (CI), -0.42, -0.12), aberrant behavior (SMD = -0.43, 95 % CI, -0.66, -0.19), hyperactivity (SMD = -0.58, 95 % CI, -1.03, -0.13), social interaction (SMD = -0.43, 95 % CI, -0.59, -0.27), social communication (SMD = -0.24, 95 % CI, -0.35, - 0.12), and restricted and repetitive behaviors (RRB) (SMD = -0.16, 95 % CI, -0.31, -0.00). Effects on irritability, anxiety, sensory sensitivity, total social skills, social awareness, social cognition, and social motivation were not statistically significant. Adverse events were similar between intervention and control groups. In conclusion, SFN shows potential in improving ASD symptoms without significant adverse effects. However, results should be interpreted cautiously due to potential influences from assessment tools, outcome assessors, and treatment duration. Further research is needed to confirm the long-term efficacy and safety of SFN for ASD.</p>","PeriodicalId":12247,"journal":{"name":"EXCLI Journal","volume":"24 ","pages":"542-557"},"PeriodicalIF":3.8,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12127520/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144208081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of immune dysregulation in the pathogenesis of type 1 diabetes: a paradigm shift in prevention strategies.","authors":"Rajiv G Gopalsamy, Hariharan Govindasamy, Jessiane B de Souza, Deise M R R Silva, Pedro H M Moura, Ronaldy S Santos, Marina Dos S Barreto, Adriana G Guimarães, Lucas A da M Santana, Lysandro P Borges, Eloia E D Silva","doi":"10.17179/excli2025-8233","DOIUrl":"https://doi.org/10.17179/excli2025-8233","url":null,"abstract":"","PeriodicalId":12247,"journal":{"name":"EXCLI Journal","volume":"24 ","pages":"539-541"},"PeriodicalIF":3.8,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12078776/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144076981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesis, structural studies, and inhibitory potential of selected sulfonamide analogues: insights from in silico and in vitro analyses.","authors":"Tahira Noor, Daniel C Schultz, Gustavo Seabra, Yuting Zhai, Kwangcheol Casey Jeong, Saleem Ahmed Bokhari, Fahim Ashraf Qureshi, Abdul Rauf Siddiqi, Chenglong Li","doi":"10.17179/excli2024-8118","DOIUrl":"https://doi.org/10.17179/excli2024-8118","url":null,"abstract":"<p><p>Antimicrobial resistance is a growing public health threat worldwide, and the current drug development pipeline has thus far been inadequate in addressing this impending crisis. Further research into antibiotic agents, both existing and novel, is therefore paramount for identifying suitable candidates to combat antibiotic-resistant pathogens. Sulfonamides, the first class of synthetic antibiotics, target dihydropteroate synthase (DHPS), a key bacterial enzyme. While this class of antibiotics has historically demonstrated great utility, their use has diminished due to resistance and undesired side effects. In the present study, we synthesized a selection of four sulfonamide analogues (<b>FQ5</b>, <b>FQ6</b>, <b>FQ7</b> and <b>FQ12</b>), validated their structures through NMR spectroscopy, and evaluated their inhibitory potential through computational docking and MIC assays against four bacterial strains: <i>Staphylococcus aureus</i> ATCC 25923, <i>Pseudomonas aeruginosa</i> ATCC 27853, <i>Escherichia coli</i> ATCC 35401 and <i>Bacillus subtilis</i> ATCC 6633. Each compound exhibited antibacterial activity; <b>FQ5</b> demonstrated the most potent activity, with an MIC of 32, 16, 16, and 16 µg/mL against aforementioned strains, respectively. <b>FQ6</b>, <b>FQ7</b> and <b>FQ12</b>, on the other hand, exhibited moderate activity against <i>P. aeruginosa</i> and <i>E. coli</i> (MIC = 128 µg/mL each) and low activity against <i>S. aureus</i> and <i>B. subtilis</i> (MIC = 256 µg/mL each). Molecular docking studies indicated that <b>FQ5</b> captures multiple hydrogen bonding, ionic, and π-π interactions with key binding pocket residues of DHPS, and <b>FQ5</b> also demonstrated superior predicted drug-likeness in <i>in silico</i> ADMET studies compared to other compounds. <b>FQ5</b> is therefore a favorable starting point for further optimization.</p>","PeriodicalId":12247,"journal":{"name":"EXCLI Journal","volume":"24 ","pages":"527-538"},"PeriodicalIF":3.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12078777/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144076979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}