EXCLI Journal最新文献

{"title":"Light-based therapies and radiodermatitis: a case series report.","authors":"Talita Oliveira de Lima, Karina Alexandra Batista da Silva Freitas, Karen Aline Batista da Silva, Maria Fernanda Setúbal Destro Rodrigues, Thais Barbosa Dos Santos, Illora Aswinkumar Darbar Shimozato, Christiane Pavani, Rebeca Boltes Cecatto","doi":"10.17179/excli2024-7749","DOIUrl":"https://doi.org/10.17179/excli2024-7749","url":null,"abstract":"<p><p>The main treatments for cancer are radiotherapy and chemotherapy, but they can generate side effects such as fatigue, myelosuppression, and radiodermatitis. The Multinational Association of Supportive Care in Cancer already recommends the use of laser for radiodermatitis in breast cancer patients. However, in relation to head and neck cancer patients, there is a lack of studies clearly demonstrating clinical effects and identifying the best light parameters for the treatment of radiodermatitis. This study reports on three oncological patients with radiodermatitis treated with light-based therapies to show clinical improvements in lesion grades and to discuss the effects of laser and its parameters. A retrospective report of three head and neck cancer patients with radiodermatitis, treated with photobiomodulation and photodynamic therapy at an outpatient health clinical facility. The Visual Analog Scale, Toxicity Criteria of the Radiation Therapy Oncology Group (RTOG) Scale, and the clinical characteristics of lesions were evaluated before and after a photobiomodulation plus photodynamic therapy protocol. Improvements were observed in cases with RTOG grade III with just 4 treatment sessions required for complete healing of the lesions. The patient with RTOG grade IV required antibiotic therapy, temporary suspension of radiotherapy, and more than 4 light sessions to achieve improvements. None of the patients showed worsening of the lesions, necrosis, or infection after treatment with no adverse effects. Head and neck cancer patients with radiodermatitis treated with phototherapy obtained good results in wound healing and pain relief in a short period. These case reports embody the easy-to-apply implementation of a light protocol in a health facility based on previous scientific evidence with positive results and no short-term side effects. In light of the negative impact on quality of life caused by radiodermatitis, health teams should be encouraged to design research study protocols involving light-based therapies.</p>","PeriodicalId":12247,"journal":{"name":"EXCLI Journal","volume":"23 ","pages":"1276-1286"},"PeriodicalIF":3.8,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11610036/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142767627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A paradigm shift in the detection of bloodborne pathogens: conventional approaches to recent detection techniques.","authors":"Sonali Khanal, Manjusha Pillai, Deblina Biswas, Muhammad Torequl Islam, Rachna Verma, Kamil Kuca, Dinesh Kumar, Asim Najmi, Khalid Zoghebi, Asaad Khalid, Syam Mohan","doi":"10.17179/excli2024-7392","DOIUrl":"10.17179/excli2024-7392","url":null,"abstract":"<p><p>Bloodborne pathogens (BBPs) pose formidable challenges in the realm of infectious diseases, representing significant risks to both human and animal health worldwide. The review paper provides a thorough examination of bloodborne pathogens, highlighting the serious worldwide threat they pose and the effects they have on animal and human health. It addresses the potential dangers of exposure that healthcare workers confront, which have affected 3 million people annually, and investigates the many pathways by which these viruses can spread. The limitations of traditional detection techniques like PCR and ELISA have been criticized, which has led to the investigation of new detection methods driven by advances in sensor technology. The objective is to increase the amount of knowledge that is available regarding bloodborne infections as well as effective strategies for their management and detection. This review provides a thorough overview of common bloodborne infections, including their patterns of transmission, and detection techniques.</p>","PeriodicalId":12247,"journal":{"name":"EXCLI Journal","volume":"23 ","pages":"1245-1275"},"PeriodicalIF":3.8,"publicationDate":"2024-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11579516/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142686480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fisetin-loaded nanoemulsion ameliorates lung cancer pathogenesis via downregulating cathepsin-B, galectin-3 and enolase in an <i>in vitro</i> setting.","authors":"Ayeh Bani Saeid, Keshav Raj Paudel, Gabriele De Rubis, Samir Mehndiratta, Sofia Kokkinis, Sukriti Vishwas, Stewart Yeung, Gaurav Gupta, Sachin Kumar Singh, Kamal Dua","doi":"10.17179/excli2024-7583","DOIUrl":"10.17179/excli2024-7583","url":null,"abstract":"","PeriodicalId":12247,"journal":{"name":"EXCLI Journal","volume":"23 ","pages":"1238-1244"},"PeriodicalIF":3.8,"publicationDate":"2024-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11579513/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142686583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Head and neck cancer care: advanced molecular and genetic diagnostics.","authors":"Rabab Fatima","doi":"10.17179/excli2024-7727","DOIUrl":"https://doi.org/10.17179/excli2024-7727","url":null,"abstract":"","PeriodicalId":12247,"journal":{"name":"EXCLI Journal","volume":"23 ","pages":"1229-1231"},"PeriodicalIF":3.8,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11610038/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142767625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combination therapy of phytonanomedicine and post-biotic for the management of Parkinson's disease.","authors":"Bushra Bashir, Sukriti Vishwas, Monica Gulati, Gaurav Gupta, Sachin Kumar Singh","doi":"10.17179/excli2024-7770","DOIUrl":"https://doi.org/10.17179/excli2024-7770","url":null,"abstract":"","PeriodicalId":12247,"journal":{"name":"EXCLI Journal","volume":"23 ","pages":"1234-1237"},"PeriodicalIF":3.8,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11609944/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142767621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling the anticancer potential of platycodin D.","authors":"Tae Kyung Hyun","doi":"10.17179/excli2024-7724","DOIUrl":"https://doi.org/10.17179/excli2024-7724","url":null,"abstract":"","PeriodicalId":12247,"journal":{"name":"EXCLI Journal","volume":"23 ","pages":"1232-1233"},"PeriodicalIF":3.8,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11609939/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142767631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"AI is revolutionizing biomedical research, but are there any negatives?","authors":"Bor Luen Tang","doi":"10.17179/excli2024-7761","DOIUrl":"https://doi.org/10.17179/excli2024-7761","url":null,"abstract":"","PeriodicalId":12247,"journal":{"name":"EXCLI Journal","volume":"23 ","pages":"1226-1228"},"PeriodicalIF":3.8,"publicationDate":"2024-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11610031/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142767617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultrastructural alterations and mitochondrial dysfunction in skeletal muscle of peripheral artery disease patients: implications for early therapeutic interventions.","authors":"Dylan Wilburn, Emma Fletcher, Evlampia Papoutsi, William T Bohannon, Gleb Haynatzki, Bernd Zechmann, Yuqian Tian, Iraklis I Pipinos, Dimitrios Miserlis, Panagiotis Koutakis","doi":"10.17179/excli2024-7592","DOIUrl":"10.17179/excli2024-7592","url":null,"abstract":"&lt;p&gt;&lt;p&gt;Peripheral artery disease (PAD) is an atherosclerotic condition that impairs blood flow to the lower extremities, resulting in myopathy in affected skeletal muscles. Improving our understanding of PAD and developing novel treatment strategies necessitates a comprehensive examination of cellular structural alterations that occur in the muscles with disease progression. Here we aimed to employ electron microscopy to quantify skeletal muscle ultrastructural alterations responsible for the myopathy of PAD. Fifty-two participants (22 controls, 10 PAD Stage II, and 20 PAD Stage IV) were enrolled. Gastrocnemius biopsies were obtained to determine mitochondrial respiration and oxidative stress. Skeletal muscle sarcomere, mitochondria, lipid droplets, and sarcoplasm were assessed using transmission electron microscopy and focused ion beam scanning electron microscopy. Controls and PAD Stage II patients underwent walking performance tests: 6-minute walking test, 4-minute walking velocity, and maximum graded treadmill test. We identified several prominent ultrastructural modifications in PAD gastrocnemius, including reduced sarcomere dimensions, alterations in mitochondria number and localization, myofibrillar disorientation, changes in lipid droplets, and modifications in mitochondria-lipid droplet contact area. These changes correlated with impaired mitochondrial respiration and increased ROS production. We observed progressive deterioration in mitochondrial parameters across PAD stages. Stage II PAD showed impaired mitochondrial function and structure, while stage IV exhibited further deterioration, more pronounced structural alterations, and a decrease in mitochondrial content. The walking performance of Stage II PAD patients was significantly reduced. Our findings suggest that pathological mitochondria play a key role in the skeletal muscle dysfunction of PAD patients and represent an important target for therapeutic interventions aimed at improving clinical and functional outcomes in this patient population. Our data indicate that treatments should be implemented early and may include therapies designed to preserve and enhance mitochondrial biogenesis and respiration, optimize mitochondrial-lipid droplet interactions, or mitigate oxidative stress. &lt;b&gt;Translational Perspective&lt;/b&gt;: Peripheral artery disease (PAD) is characterized by skeletal muscle and mitochondrial dysfunction. Ultrastructural changes in skeletal muscle myofibers and mitochondria morphology can provide significant information on the PAD pathophysiology. Here, we investigated skeletal muscle and mitochondria morphological and functional changes at the sarcomere level and across the disease progression and have found that sarcomere lengths and mitochondria count and function are associated with disease progression, indicating loss of skeletal muscle contractile and metabolic function. Ultrastructural changes in the PAD skeletal muscle can provide significant information in the dev","PeriodicalId":12247,"journal":{"name":"EXCLI Journal","volume":"23 ","pages":"1208-1225"},"PeriodicalIF":3.8,"publicationDate":"2024-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11579521/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142686585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimized therapeutic potential of Sijunzi-similar formulae for chronic atrophic gastritis via Bayesian network meta-analysis.","authors":"Meilan Huang, Shiman Luo, Jiayue Yang, Huiling Xiong, Xiaohua Lu, Xiao Ma, Jinhao Zeng, Thomas Efferth","doi":"10.17179/excli2024-7618","DOIUrl":"https://doi.org/10.17179/excli2024-7618","url":null,"abstract":"<p><p>Chronic atrophic gastritis (CAG) is considered as a significant risk factor for triggering gastric cancer incidence, if not effectively treated. <i>Sijunzi</i> decoction (SD) is a well-known classic formula for treating gastric disorders, and <i>Sijunzi</i>-similar formulae (SF) derived from SD have also been highly regarded by Chinese clinical practitioners for their effectiveness in treating chronic atrophic gastritis. Currently, there is a lack of meta-analysis for these formulae, leaving unclear which exhibits optimal efficacy. Therefore, we employed Bayesian network meta-analysis (BNMA) to evaluate the efficacy and safety of SF as an intervention for CAG and to establish a scientific foundation for the clinical utilization of SF. The result of meta-analysis demonstrated that the combination of SF and basic therapy outperformed basic therapy alone in terms of clinical efficacy rate, eradication rate of H. pylori, and incidence of adverse events. As indicated by the SUCRA value, <i>Chaishao Liujunzi</i> decoction (CLD) demonstrated superior efficacy in enhancing clinical effectiveness and ameliorating <i>H. pylori</i> infection, and it also showed remarkable effectiveness in minimizing the occurrence of adverse events. Comprehensive analysis of therapeutic efficacy suggests that CLD is most likely the optimal choice among these six formulations, holding potential value for optimizing clinical treatment strategies. See also the graphical abstract(Fig. 1).</p>","PeriodicalId":12247,"journal":{"name":"EXCLI Journal","volume":"23 ","pages":"1185-1207"},"PeriodicalIF":3.8,"publicationDate":"2024-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11484511/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142461529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pitfalls in the use of sex chromosome sequence markers for internal quality control of next-generation sequencing.","authors":"Danielle Patchell, Stephen E Langabeer","doi":"10.17179/excli2024-7387","DOIUrl":"10.17179/excli2024-7387","url":null,"abstract":"","PeriodicalId":12247,"journal":{"name":"EXCLI Journal","volume":"23 ","pages":"1183-1184"},"PeriodicalIF":3.8,"publicationDate":"2024-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11464863/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142399903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}