Experimental Dermatology最新文献_第2页

Sensitive Skin Syndrome in the Chinese Population—A Critical Discussion of Current Knowledge, Clinical Implications and Research Needs 敏感性皮肤综合征在中国人群-当前知识，临床意义和研究需求的关键讨论

IF 3.5 3区医学

Experimental Dermatology Pub Date : 2025-06-16 DOI: 10.1111/exd.70124

Thomas Jaenicke, Tao Zhang, Ehrhardt Proksch, Jean Krutmann, Thomas Luger

引用次数: 0

Skin Pressing: An Easy and Reliable Method to Induce Acute Hair Greying in Mice and Useful for Studying Canities 皮肤按压：一种简便可靠的诱导小鼠急性毛发变白的方法，对研究犬科动物有益

IF 3.5 3区医学

Experimental Dermatology Pub Date : 2025-06-09 DOI: 10.1111/exd.70126

Yoshihiro Kawai, Ichitaro Niibe, Takashi Matsuzaki

{"title":"Skin Pressing: An Easy and Reliable Method to Induce Acute Hair Greying in Mice and Useful for Studying Canities","authors":"Yoshihiro Kawai, Ichitaro Niibe, Takashi Matsuzaki","doi":"10.1111/exd.70126","DOIUrl":"https://doi.org/10.1111/exd.70126","url":null,"abstract":"Grey hair is a hallmark of aging, and its restoration is a major concern. Using aged mice for studying hair greying has disadvantages, such as individual variability and time requirements. In this study, we developed a simple method to induce acute and less variable hair greying in mice. After induction of the hair cycle anagen in black mice, the dorsal skin was pinched and pressed from both sides using a pair of C-shaped neodymium magnets cushioned with a thin silicone rubber. This pressure was maintained for 8 h. Grey hair was consistently observed on the skin just below the area where the skin press was applied and sporadically inside, but not outside, the area in all treated mice. No obvious differences were observed in hair characteristics (length, thickness, shape, and hair-type ratio) between the induced grey hair and normal black hair, except for a slight delay in hair emergence. The sporadic greying pattern resembled that of age-related hair depigmentation. The grey pattern was stable through three consecutive hair cycles, and melanocyte stem cells were observed in the bulge area of the grey hair, suggesting that the skin press method offers a reliable model for studying the mechanisms and treatment of canities.","PeriodicalId":12243,"journal":{"name":"Experimental Dermatology","volume":"34 6","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/exd.70126","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144244385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spatial Transcriptomics Shows a Distinctive Tumour Microenvironment in the Invasive Versus Premalignant Portion of Early Cutaneous Squamous Cell Carcinoma 空间转录组学显示了早期皮肤鳞状细胞癌侵袭性和癌前部分不同的肿瘤微环境

IF 3.5 3区医学

Experimental Dermatology Pub Date : 2025-06-03 DOI: 10.1111/exd.70125

Jeong-An Gim, Chungyeul Kim, Hyun Jyung Oh, Ko Eun Kim, Jiehyun Jeon, Aeree Kim, Yoo Sang Baek

{"title":"Spatial Transcriptomics Shows a Distinctive Tumour Microenvironment in the Invasive Versus Premalignant Portion of Early Cutaneous Squamous Cell Carcinoma","authors":"Jeong-An Gim, Chungyeul Kim, Hyun Jyung Oh, Ko Eun Kim, Jiehyun Jeon, Aeree Kim, Yoo Sang Baek","doi":"10.1111/exd.70125","DOIUrl":"https://doi.org/10.1111/exd.70125","url":null,"abstract":"Cutaneous squamous cell carcinoma (SCC) is known for its stepwise progression from healthy skin to premalignant actinic keratosis (AK), followed by a malignant transformation to SCC. Unfortunately, less attention has been paid to changes in gene expression in the tumour microenvironment during this process. We retrospectively selected early-stage cutaneous SCC tissue samples containing both invasive and premalignant portions and conducted a spatial transcriptomic experiment using a NanoString GeoMx Digital Spatial Profiler (DSP). First, we selected invasive and premalignant regions of interest (ROIs) for each tissue. We then compared the gene expression patterns between the two portions (invasive versus premalignant) of the three segments: tumour cells, immune cells and fibroblasts, in each ROI. As a result, early-stage cutaneous SCC tissue samples from 17 patients were selected for this study. We identified 29, 14 and 15 differentially expressed genes (DEGs) between the invasive and premalignant portions of the tumour cells, immune cells and fibroblasts, respectively. The top three genes with the highest absolute log2 fold-change were CCDC88C, GJD3 and COMP in tumour cells; SVEP1, TSLP and PPP2R5C in immune cells; and SPAG6, PPP1CA and CCDC68 in fibroblasts. Notably, several genes, such as COMP, SVEP1 and SPAG6, have been linked to the development and function of cancer-associated fibroblasts. Functional enrichment analysis revealed that several pathways were altered in tumour and immune cells. In conclusion, distinctive changes in gene expression patterns were observed as AK progressed to SCC.","PeriodicalId":12243,"journal":{"name":"Experimental Dermatology","volume":"34 6","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/exd.70125","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144206651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

JAK1/2 Inhibitors Alleviate the Damage of Intercellular Adhesion by Reducing Endoplasmic Reticulum Stress-Induced Apoptosis in Pemphigus Vulgaris JAK1/2抑制剂通过减少内质网应激诱导的细胞凋亡减轻寻常型天疱疮细胞间粘附损伤

IF 3.5 3区医学

Experimental Dermatology Pub Date : 2025-05-20 DOI: 10.1111/exd.70121

Xi Chen, Mei-Nian Xu, Zheng-Quan Wu, Rong Huang, Hong-Yan Lu, Xiaoming Peng, Kang Zeng, Chang-Xing Li

{"title":"JAK1/2 Inhibitors Alleviate the Damage of Intercellular Adhesion by Reducing Endoplasmic Reticulum Stress-Induced Apoptosis in Pemphigus Vulgaris","authors":"Xi Chen, Mei-Nian Xu, Zheng-Quan Wu, Rong Huang, Hong-Yan Lu, Xiaoming Peng, Kang Zeng, Chang-Xing Li","doi":"10.1111/exd.70121","DOIUrl":"https://doi.org/10.1111/exd.70121","url":null,"abstract":"<div>\u0000 \u0000 Pemphigus vulgaris (PV), a severe autoimmune disease with high morbidity and mortality, necessitates innovative therapies to improve outcomes while minimising the adverse effects of conventional immunosuppressants. Immunohistochemical analysis revealed elevated phosphorylated Janus kinase (p-JAK)1 and p-JAK2 expression in PV lesions, complemented by transcriptome data showing JAK/STAT pathway dysregulation. Using a PV acantholysis model, we demonstrated that Ruxolitinib, a JAK1/2 inhibitor, significantly reduced keratinocyte apoptosis, enhanced cell adhesion, and alleviated endoplasmic reticulum (ER) stress. Additionally, Ruxolitinib mitigated tunicamycin-induced ER stress and apoptosis in HaCaT cells. These findings establish a crucial role for JAK1/2 in PV pathogenesis, demonstrating that their inhibition alleviates ER stress, reduces apoptosis, and improves cell adhesion. Our results provide a theoretical foundation for the clinical application of JAK inhibitors in PV treatment.\u0000 </div>","PeriodicalId":12243,"journal":{"name":"Experimental Dermatology","volume":"34 5","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144091892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nemolizumab Improves Pruritus in Patients With Intrinsic Atopic Dermatitis Lacking Atopic Predisposition: A Single-Centre Pilot Retrospective Cohort Study 奈莫单抗改善缺乏特应性易感性的内在特应性皮炎患者的瘙痒：一项单中心试点回顾性队列研究

IF 3.5 3区医学

Experimental Dermatology Pub Date : 2025-05-15 DOI: 10.1111/exd.70120

Emi Sato, Hisatomi Arima, Keita Tsutsui, Hiroki Shimizu, Kotaro Ito, Mayuko Iwata, Shinichi Imafuku

{"title":"Nemolizumab Improves Pruritus in Patients With Intrinsic Atopic Dermatitis Lacking Atopic Predisposition: A Single-Centre Pilot Retrospective Cohort Study","authors":"Emi Sato, Hisatomi Arima, Keita Tsutsui, Hiroki Shimizu, Kotaro Ito, Mayuko Iwata, Shinichi Imafuku","doi":"10.1111/exd.70120","DOIUrl":"https://doi.org/10.1111/exd.70120","url":null,"abstract":"<div>\u0000 \u0000 Interleukin (IL)-31 is a key therapeutic target for severe pruritus in atopic dermatitis (AD). Nemolizumab, an IL-31 receptor A antibody, has been available in Japan since 2022 for treating AD-related pruritus. This retrospective study aimed to identify the characteristics of patients with AD for whom nemolizumab is appropriate and most effective, focusing on its efficacy in alleviating pruritus. We reviewed the clinical data of patients with AD who received 60 mg of nemolizumab between 2022 and 2024 at Fukuoka University Hospital. Patients who achieved a ≥ 4-point improvement on the Peak Pruritus Numerical Rating Scale (PP-NRS4) within 16 weeks were classified as responders. Background characteristics, including atopic predisposition and total serum immunoglobulin E (IgE) levels, were compared between responders and non-responders. Multivariate analysis was performed to identify treatment response predictors. Sixteen (64%) of the 25 patients treated with nemolizumab achieved PP-NRS4 within 16 weeks. Of the 25 patients, 14 had extrinsic AD with an atopic predisposition, although only 5 (36%) achieved PP-NRS4. All 11 patients with intrinsic AD achieved PP-NRS4 (p = 0.001). The median total serum IgE level was significantly lower in responders (74.5 IU/mL) than in non-responders (691.5 IU/mL, p = 0.0034). Multivariate analysis revealed that higher baseline IgE levels were associated with poorer PP-NRS4 outcomes (standardised β = −0.63033, p = 0.0154). Serum total IgE levels, indicative of an atopic predisposition, are critical predictors of nemolizumab efficacy in alleviating pruritus. These findings underscore the importance of patient selection based on IgE levels for optimising nemolizumab therapy in AD.\u0000 </div>","PeriodicalId":12243,"journal":{"name":"Experimental Dermatology","volume":"34 5","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143950151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Memory T-Cell Phenotype in Cutaneous T-Cell Lymphoma Is Modified by Germline Gene Gametocyte Specific Factor 1 种系基因配子细胞特异性因子1修饰皮肤t细胞淋巴瘤的记忆t细胞表型

IF 3.5 3区医学

Experimental Dermatology Pub Date : 2025-05-14 DOI: 10.1111/exd.70123

Amelia Martínez Villarreal, Jennifer Gantchev, Pingxing Xie, Philippe Lefrançois, Brandon Ramchatesingh, Ivan V. Litvinov

{"title":"Memory T-Cell Phenotype in Cutaneous T-Cell Lymphoma Is Modified by Germline Gene Gametocyte Specific Factor 1","authors":"Amelia Martínez Villarreal, Jennifer Gantchev, Pingxing Xie, Philippe Lefrançois, Brandon Ramchatesingh, Ivan V. Litvinov","doi":"10.1111/exd.70123","DOIUrl":"https://doi.org/10.1111/exd.70123","url":null,"abstract":"Cutaneous T-cell lymphoma (CTCL) is a heterogeneous group of lymphoproliferative disorders characterised by skin infiltration by malignant memory T cells. While most patients will present with an indolent disease, others will follow a highly aggressive clinical course. Currently, defining disease prognosis remains challenging. Ectopic expression of gametocyte-specific factor 1 (GTSF1) has emerged as a potential prognostic biomarker. However, its contribution to CTCL carcinogenesis remains unknown. Here, we report that GTSF1 contributes to carcinogenesis by partially modifying the memory/effector phenotype of the malignant T cells. GTSF1 knockdown in CTCL cells led to T-cell activation and production of IFNγ and TNFα. Advanced stages of the disease are associated with decreased production of these cytokines. Notably, we show that patients classified with high expression of GTSF1 are associated with a worse disease prognosis. Taken together, our findings indicate that GTSF1 expression in CTCL cells allows them to acquire memory T-cell phenotype. Malignant memory T cells have a decreased production of immune-responsive cytokines, leading to a diminished immune response and disease progression. GTSF1 is an important candidate as a prognostic biomarker. Furthermore, understanding the specific function of GTSF1 might help develop novel targeted treatment options for CTCL patients.","PeriodicalId":12243,"journal":{"name":"Experimental Dermatology","volume":"34 5","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/exd.70123","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143949773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Extension of Secukinumab and Ixekizumab Dose for Moderate-To-Severe Psoriasis in Low Disease Activity Intervals 在低疾病活动期延长Secukinumab和Ixekizumab治疗中重度银屑病的剂量

IF 3.5 3区医学

Experimental Dermatology Pub Date : 2025-05-14 DOI: 10.1111/exd.70122

Caiyu Wang, Yanhua Liu, Yang Yang, Jiao Ning, Xiaoyi Xing, Ping Jian, Ziyuan Wang, Guangquan Xu, Zhongrui Xu, Jian Zhou, Xiaoming Liu, Chen Yu, Gang Wang

{"title":"Extension of Secukinumab and Ixekizumab Dose for Moderate-To-Severe Psoriasis in Low Disease Activity Intervals","authors":"Caiyu Wang, Yanhua Liu, Yang Yang, Jiao Ning, Xiaoyi Xing, Ping Jian, Ziyuan Wang, Guangquan Xu, Zhongrui Xu, Jian Zhou, Xiaoming Liu, Chen Yu, Gang Wang","doi":"10.1111/exd.70122","DOIUrl":"https://doi.org/10.1111/exd.70122","url":null,"abstract":"<div>\u0000 \u0000 Biological agents targeting IL-17A, such as secukinumab and ixekizumab, are highly effective in treating psoriasis, often achieving low disease activity or remission. However, frequent injections, side effects and high costs pose significant challenges. Extending dosing intervals for patients with stable disease and partial response may address these issues while maintaining efficacy, yet standardised dose reduction guidelines remain absent. This study aimed to evaluate the efficacy and safety of reduced dosing regimens of secukinumab and ixekizumab during the maintenance phase of psoriasis treatment. From 2020 to 2023, patients completing induction and maintenance phases with prescribed regimens, achieving a PASI (Psoriasis Area and Severity Index) score below 1 and PASI90 or PASI100 response, underwent clinician evaluations for extended dosing intervals. Secukinumab dosing was adjusted from 300 mg (or 150 mg) Q4W to Q8W, and ixekizumab from 80 mg Q4W to Q8W. Patients were monitored over 36 weeks, with data collected throughout the observation period to assess feasibility and safety. A total of 64 patients with moderate-to-severe plaque psoriasis were enrolled. Following extended dosing intervals, 75.4% maintained a PASI90 response at 12 weeks, with 67.7% sustaining it at 36 weeks. Similarly, 69.8% achieved PASI100 at 12 weeks, and 61.2% maintained this response at 36 weeks. These findings demonstrate that dose reduction strategies for secukinumab and ixekizumab in moderate-to-severe psoriasis can reduce treatment burden while maintaining high therapeutic efficacy, offering valuable insights to guide clinical guidelines and address current knowledge gaps.\u0000 </div>","PeriodicalId":12243,"journal":{"name":"Experimental Dermatology","volume":"34 5","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143949774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characteristics of the MicroRNA-Processing Enzymes in Melanocytic Skin Lesions: Dicer and DGCR8 Are Potential Biomarkers for Primary Cutaneous Melanomas 黑色素细胞性皮肤病变中microrna加工酶的特征：Dicer和DGCR8是原发性皮肤黑色素瘤的潜在生物标志物

IF 3.5 3区医学

Experimental Dermatology Pub Date : 2025-05-08 DOI: 10.1111/exd.70110

Fabiola Schafer, Enrique Bellolio, Tatiana Sepúlveda, Mirta Espinoza, Juan-José Orellana, Isabel Bellolio, Miguel Angel Villaseca, Rodrigo Miranda

{"title":"Characteristics of the MicroRNA-Processing Enzymes in Melanocytic Skin Lesions: Dicer and DGCR8 Are Potential Biomarkers for Primary Cutaneous Melanomas","authors":"Fabiola Schafer, Enrique Bellolio, Tatiana Sepúlveda, Mirta Espinoza, Juan-José Orellana, Isabel Bellolio, Miguel Angel Villaseca, Rodrigo Miranda","doi":"10.1111/exd.70110","DOIUrl":"https://doi.org/10.1111/exd.70110","url":null,"abstract":"<div>\u0000 \u0000 Primary cutaneous melanoma (PCM) is an aggressive skin cancer. Its physiopathology is a challenge with heterogeneous pathways involved. As such, microRNA-processing enzymes have been shown to be deregulated in cancer. The aim of this study was to characterise the expression profile of Dicer, Drosha, DGCR8 and PACT enzymes in melanocytic skin lesions. A total of 126 formalin-fixed paraffin-embedded samples, including 42 benign nevi, 42 dysplastic nevi and 42 PCM, were studied using tissue microarray and immunohistochemistry, which was graded based on the percentage of immunoreactive tumour cells (%IRC). Increased Dicer immunoexpression was found in PCM compared to benign nevi (p = 0.044) and increased DGCR8 immunoexpression was found in PCM compared to dysplastic and benign nevi (p = 0.000). For Drosha and PACT, only dysplastic nevi showed an increased expression (p = 0.011). A ROC curve cut-off of 80% IRC was used. For Dicer, the specificity for non-malignant cutaneous lesions (NMCL) was 98.8%, and sensitivity for PCM was 31.0%. The negative predictive value (NPV) was 98.6% and positive predictive value (PPV) was 34.7%. For DGCR8, the specificity for NMCL was 100%, and sensitivity for PCM was 31.0%. The NPV was 98.6% and PPV was 100%. All cases with positive Dicer and DGCR8 immunoexpression were melanomas. Dicer was increased in nodular histologic subtype (p = 0.011) and DGCR8 was higher in males (p = 0.005). Both Dicer and DGCR8 were increased in ulcerated PCM (p < 0.05). Dicer and DGCR8 play an important role in melanoma development with a potential use as diagnostic tools to differentiate PCM from other melanocytic skin lesions.\u0000 </div>","PeriodicalId":12243,"journal":{"name":"Experimental Dermatology","volume":"34 5","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143925782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Blocking of IL-4/IL-13 Signalling With Dupilumab Results in Restoration of Serum and Cutaneous Abnormalities in Netherton Syndrome 用Dupilumab阻断IL-4/IL-13信号传导可恢复内瑟顿综合征的血清和皮肤异常

IF 3.5 3区医学

Experimental Dermatology Pub Date : 2025-05-08 DOI: 10.1111/exd.70113

Stefan Blunder, Natascha Hermann-Kleiter, Rita Mahmuti, Martin Hermann, Daniela Ortner, Daniela Reider, Verena Moosbrugger-Martinz, Barbara Del Frari, Patrizia Stoitzner, Sandrine Dubrac, Matthias Schmuth, Robert Gruber

{"title":"Blocking of IL-4/IL-13 Signalling With Dupilumab Results in Restoration of Serum and Cutaneous Abnormalities in Netherton Syndrome","authors":"Stefan Blunder, Natascha Hermann-Kleiter, Rita Mahmuti, Martin Hermann, Daniela Ortner, Daniela Reider, Verena Moosbrugger-Martinz, Barbara Del Frari, Patrizia Stoitzner, Sandrine Dubrac, Matthias Schmuth, Robert Gruber","doi":"10.1111/exd.70113","DOIUrl":"https://doi.org/10.1111/exd.70113","url":null,"abstract":"<div>\u0000 \u0000 Netherton syndrome (NS) is a rare ichthyosis caused by SPINK5-null mutations, resulting in erythroderma, ichthyosis linearis circumflexa, and atopic diathesis. Elevated serum IgE levels and activation of the KLK5-PAR2-TSLP axis suggest involvement of Th2-skewed immunity in NS. In this pilot study, we investigated the effects of IL-4/IL-13 blocking with dupilumab on NS features. At baseline, Th2-chemokines CCL11, CCL17, CCL18, CCL26, and serum IgE were more elevated in atopic dermatitis (AD) than in NS vs. controls (ctrls). AD exhibited elevated serum levels of CCL27, LDH, and eosinophils, while NS showed higher levels of IL-9 and IL-18. Epidermal aberrations, including acanthosis and SC-detachment, were present in NS versus ctrls. The number of CD3+ T cells increased, while CD1a + Langerhans cell numbers decreased in NS skin. Amounts of KLK5 were reduced, and the distribution of KLK7 was abnormal in NS epidermis as compared to ctrls. Reduced amounts of FLG, CDSN, and DSG1 highlight impaired keratinocyte late differentiation in NS. Amounts of epidermal TSLP were diminished. Upon dupilumab treatment, clinical improvement in NS began as early as week 8 and continued up to 30 months, with no serious side effects reported. Serum levels of IgE, CCL17, CCL26, IFN-γ and IL-18 decreased upon IL-4/IL-13 blockade, and alterations of cutaneous immune cells improved in NS. Furthermore, the epidermal protease inhibitor WFDC12 expression increased after dupilumab treatment, concurring with improved and partially normalised epidermal structure, including increased FLG, CDSN, and DSG1. These data highlight Th2-skewed immunity in NS and emphasise the amelioration of NS features through dupilumab treatment.\u0000 </div>","PeriodicalId":12243,"journal":{"name":"Experimental Dermatology","volume":"34 5","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143925783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Generation of a Novel Inducible and Dermal Papilla-Specific Wif1-CreER Knock-In Mouse Line for Hair Follicle Research 用于毛囊研究的新型诱导和真皮乳头特异性wip1 - creer敲入小鼠系的产生

IF 3.5 3区医学

Experimental Dermatology Pub Date : 2025-05-07 DOI: 10.1111/exd.70109

Rina Su, Guangqian Shen, Xin Xiao, Yinghui Zheng, Fang Liu, Daoming Chen

{"title":"Generation of a Novel Inducible and Dermal Papilla-Specific Wif1-CreER Knock-In Mouse Line for Hair Follicle Research","authors":"Rina Su, Guangqian Shen, Xin Xiao, Yinghui Zheng, Fang Liu, Daoming Chen","doi":"10.1111/exd.70109","DOIUrl":"https://doi.org/10.1111/exd.70109","url":null,"abstract":"<div>\u0000 \u0000 Dermal papilla (DP) cells are essential niche cells that regulate hair follicle development, cycling and regeneration. Despite the establishment of several DP cell mouse lines in prior research, these tools are limited by incomplete specificity and spatiotemporal control. The Wnt inhibitory factor 1 (Wif1) has been identified as a DP signature gene. To address the need for precise labelling and manipulation of DP cells, we developed a novel genetic tool—Wif1-CreER knock-in mice. Using CRISPR/Cas9-mediated homologous recombination, the CreERT2 sequences were inserted into the endogenous Wif1 locus, under the control of the native promoter. PCR and sequencing analysis confirmed the accurate insertion of the CreERT2 sequence. Crossing Wif1-CreER mice with a reporter line demonstrated efficient and specific Cre recombinase activity in DP cells during anagen, catagen and telogen upon tamoxifen treatment across hair types. Importantly, DP-restricted labelling was confirmed by immunofluorescence and colocalised with Crabp1 and alkaline phosphatase (AP)-staining activity, exhibiting minimal to negligible expression in other tissues. This innovative mouse model overcomes the limitations of current tools and provides a valuable resource for advancing our understanding of hair biology and developing targeted therapies for hair-related disorders, offering unprecedented precision in the manipulation of dermal papilla cells.\u0000 </div>","PeriodicalId":12243,"journal":{"name":"Experimental Dermatology","volume":"34 5","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143914046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0