Experimental Dermatology最新文献

{"title":"The role of macrophage migration inhibitory factor family and CD74 in the pathogenesis of melanoma","authors":"Keiji Tanese,&nbsp;Dai Ogata","doi":"10.1111/exd.15122","DOIUrl":"10.1111/exd.15122","url":null,"abstract":"<p>Melanoma is an aggressive tumour with poor prognosis that arises from the malignant transformation of melanocytes. Over the past few decades, intense research into the pathogenesis of melanoma has led to the development of BRAF and immune checkpoint inhibitors, including antibodies against programmed cell death protein 1 (PD-1) and cytotoxic T lymphocyte-associated protein 4 (CTLA-4), which have shown clinically significant efficacy. However, some tumours do not respond to these therapies initially or become treatment resistant. Most melanoma tissues appear to possess biological characteristics that allow them to evade these treatments, and identifying these characteristics is one of the major challenges facing cancer researchers. One such characteristic that has recently gained attention is the role of macrophage migration inhibitory factor (MIF) and its receptor CD74. This review outlines the cellular and molecular functions of CD74, MIF and their family of proteins. We then review their roles in tumours based on previous reports, highlight their pathological significance in melanoma and discuss their potential as therapeutic targets.</p>","PeriodicalId":12243,"journal":{"name":"Experimental Dermatology","volume":"33 6","pages":""},"PeriodicalIF":3.6,"publicationDate":"2024-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/exd.15122","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141330707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated bioinformatics analysis and experimental validation identifies CPE as a potential biomarker and therapeutic target for skin aging","authors":"Xiaozhen Peng,&nbsp;Yun Zhong,&nbsp;Rui Mao,&nbsp;Fanping He,&nbsp;Yufan Cheng,&nbsp;Mengting Chen,&nbsp;Lei Zhou,&nbsp;Hongfu Xie,&nbsp;Ji Li,&nbsp;Yiya Zhang","doi":"10.1111/exd.15120","DOIUrl":"10.1111/exd.15120","url":null,"abstract":"<p>Ageing is an inevitable biological process characterized by progressive decline in physiological functions. It is a complex natural phenomenon that will cause structural and functional decline. Despite substantial progress in understanding the mechanism of ageing, both predictive biomarkers and preventive therapies remain limited. Using Weighted Gene Co-expression Network Analysis (WGCNA) and machine learning techniques, we identified Carboxypeptidase E (CPE) as a pivotal marker of skin ageing, based on ageing-related bulk transcriptome and single-cell transcriptome data. Next, our investigation reveals downregulation of CPE in replicative, UVA-induced, and H₂O₂-induced senescent human dermal fibroblast cells (HDFs). Furthermore, shRNA-mediated CPE knockdown induced HDFs senescence, and overexpression of CPE delayed HDFs senescence. Moreover, downregulated CPE inhibits collagen synthesis and induces inflammation, highlighting its potential as a therapeutic target for skin ageing. In conclusion, our study demonstrated that CPE functions as a predictor and optional target for therapeutic intervention of skin ageing.</p>","PeriodicalId":12243,"journal":{"name":"Experimental Dermatology","volume":"33 6","pages":""},"PeriodicalIF":3.6,"publicationDate":"2024-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141418502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Possible role of β-hydroxybutyrate in inducing inflammation in alopecia areata","authors":"Jung-Min Shin,&nbsp;Seungjin Son,&nbsp;Kyung Eun Jung,&nbsp;Chang Deok Kim,&nbsp;Young Lee","doi":"10.1111/exd.15117","DOIUrl":"10.1111/exd.15117","url":null,"abstract":"<p>Alopecia areata (AA) is an autoimmune inflammatory disease characterized by non-scarring hair loss due to an immune response that targets hair follicles. The current treatment approach for AA involves the use of immunosuppressants and immunomodulators to reduce cytokine levels around affected hair follicles. Sodium-glucose cotransporter 2 (SGLT2) inhibitors have emerged as potential anti-inflammatory agents with diverse beneficial effects in various medical conditions. This study investigates the role of beta-hydroxybutyrate (BHB), a ketone body produced during SGLT2 inhibition, in the pathogenesis of AA. Serum BHB levels were found to be significantly elevated in patients with AA compared with healthy controls, with higher levels correlating with severity of hair loss. BHB treatment increased inflammatory cytokine production in outer root sheath (ORS) cells, mimicking the inflammatory conditions seen in AA. The results suggest that elevated BHB levels may exacerbate the inflammatory immune response in AA patients and may be associated with chronic hair loss and resistance to treatment. Serum BHB levels may serve as a potential marker of poor prognosis in patients with severe AA. Further research is needed to elucidate the precise role of BHB in the pathogenesis of AA and its implications for disease management.</p>","PeriodicalId":12243,"journal":{"name":"Experimental Dermatology","volume":"33 6","pages":""},"PeriodicalIF":3.6,"publicationDate":"2024-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/exd.15117","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141330705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Subclinical effects of botulinum toxin A and microwave thermolysis for axillary hyperhidrosis: A descriptive study with line-field confocal optical coherence tomography and histology","authors":"Gabriela Lladó Grove,&nbsp;Kevin Jacobsen,&nbsp;Nina Loeth Maartensson,&nbsp;Merete Haedersdal","doi":"10.1111/exd.15110","DOIUrl":"10.1111/exd.15110","url":null,"abstract":"<p>Botulinum toxin A (BTX) and microwave thermolysis (MWT) are standard axillary hyperhidrosis treatments, but comparison of their subclinical effects is lacking. Line-field confocal optical coherence tomography (LC-OCT) is a promising non-invasive imaging tool for visualizing tissue-interactions. This study aimed to describe subclinical effects of BTX and MWT for axillary hyperhidrosis with LC-OCT-imaging compared to histology. This study derived from an intra-individual, randomized, controlled trial, treating axillary hyperhidrosis with BTX versus MWT. Subclinical effects based on LC-OCT images from baseline and 6-month follow-up (<i>n</i> = 8 patients) were evaluated and compared to corresponding histological samples. At baseline, LC-OCT visualized eccrine pores at the skin surface and ducts in the upper dermis (500 μm), but not deeper-lying sweat glands. Histology identified entire sweat glands. Six months post-treatment, LC-OCT revealed no detectable morphology changes in any BTX-treated axillae (100%), while recognizing obstructed eccrine pores and atrophy of eccrine ducts in most MWT-treated axillae (75%). Histology corroborated LC-OCT findings, while also showing substantial changes to entire sweat glands. LC-OCT enabled visualization of subclinical alterations of superficial eccrine ducts after MWT and unchanged morphology after BTX. LC-OCT is a promising tool for non-invasive assessment of treatment-specific tissue-interactions that can be complementary to histology.</p>","PeriodicalId":12243,"journal":{"name":"Experimental Dermatology","volume":"33 6","pages":""},"PeriodicalIF":3.6,"publicationDate":"2024-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/exd.15110","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141330706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"miR-4521—A novel biomarker for human lentigos","authors":"Hiroshi Maeno,&nbsp;Yoko Suzuki-Horiuchi,&nbsp;Ayaka Funakoshi,&nbsp;Onuma Chumsakul,&nbsp;Yasunari Sato,&nbsp;Tsuyoshi Ishii,&nbsp;John T. Seykora","doi":"10.1111/exd.15118","DOIUrl":"10.1111/exd.15118","url":null,"abstract":"&lt;p&gt;Lentigos are hyperpigmented skin lesions that can vary in size and shape, typically appearing on the face, hands and shoulders, usually on sun exposed skin. Currently, the molecular pathogenesis of lentigos remains unclear. As a first step in identifying potential biomarkers of lentigos that may drive lentigo formation, we performed laser-capture microdissection (LCM) on the lesional epidermis of lentigos from five archived patient samples and, as a control, from the epidermis of three unremarkable skin samples matching for site, sex and age (Table S1). Total RNA was extracted from the micro-dissected lentigo and control tissue samples to perform transcriptomic microarray analysis (ThermoFisher Human transcriptome Array 2.0.) to identify potential biomarkers of lentigos. Bioinformatics analysis of data obtained from the whole transcriptome array (ThermoFisher TAC4.0 software package, Figure S1) identified coding and non-coding transcripts that were significantly differentially expressed in lentigos; some of these differentially expressed transcripts are associated with melanogenesis which is consistent with previous studies (Table S2).&lt;span&gt;&lt;sup&gt;1, 2&lt;/sup&gt;&lt;/span&gt; Multiple non-coding RNAs were found to be differentially regulated in the top 10 differentially expressed genes, including small nucleolar RNA species (snoRNA) and micro-RNA species (miR) (Tables S2 and S3). Studies have shown evidence that microRNAs play important biological roles ranging from development, differentiation, tumour progression or suppression.&lt;span&gt;&lt;sup&gt;3&lt;/sup&gt;&lt;/span&gt; In our data, one of the most highly expressed microRNAs was miR-4521, which is recently reported to act as a “tumor suppressor miR” to decrease the expression of pro-oncogenic targets such as forkhead box protein M1 and insulin-like growth factor 2.&lt;span&gt;&lt;sup&gt;4&lt;/sup&gt;&lt;/span&gt; Therefore, we further characterized the expression pattern of has-miR-4521 to validate it as a biomarker of lentigo.&lt;/p&gt;&lt;p&gt;The expression of miR-4521 in lentigos was evaluated by qRT-PCR and miR RNA in situ hybridization. miR-4521 expression level was 3.61 higher than that of control epidermis by qRT-PCR (Figure 1A, Mann–Whitney test, lentigo &lt;i&gt;n&lt;/i&gt; = 6, control &lt;i&gt;n&lt;/i&gt; = 4, &lt;i&gt;p&lt;/i&gt; &lt; 0.05). miR-4521 RNA in situ hybridization assays demonstrated stronger positive signals for the miR-4521 anti-sense probe in the epidermis between the stratum basalis and the stratum granulosum (Figure 1 E,G and H) compared to matched control skin (Figure 1D,F). In addition, the signal intensity of the anti-sense probe in the lentigo epidermis (Figure 1H) was higher than that of adjacent un-remarkable epidermis (Figure 1G) and matched control (Figure 1F). Together, these data indicate that miR-4521 expression is increased in lentigos primarily in the lesional keratinocytes. Melanocyte numbers were determined in all samples and there was no statistically significant difference noted (lentigo; 2.74 ± 0.37 SE /0.1 mm, control; 3.71 ± 0.21 SE / 0.1 mm, ","PeriodicalId":12243,"journal":{"name":"Experimental Dermatology","volume":"33 6","pages":""},"PeriodicalIF":3.6,"publicationDate":"2024-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/exd.15118","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141316927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Malassezia specific IgE in head and neck dermatitis of eczema: A systematic review & meta-analysis","authors":"Hui Xin See Tow,&nbsp;Yik Weng Yew","doi":"10.1111/exd.15108","DOIUrl":"10.1111/exd.15108","url":null,"abstract":"<p>Head and neck atopic dermatitis (HNAD) is a subtype of atopic dermatitis (AD), a common inflammatory skin condition with a distinctive clinical appearance. <i>Malassezia</i> spp., a predominant skin yeast, is considered to exacerbate HNAD. In this study, we investigate the prevalence of <i>Malassezia</i>-specific IgE among HNAD patients. A comprehensive search was performed for observational studies analysing the association between <i>Malassezia</i>-specific IgE and HNAD. This study was performed according to the Preferred Reporting Items for Systematic reviews and Meta-Analyses 2020 checklist and quality was assessed via the Newcastle-Ottawa Quality Assessment Scale (NOS). Fourteen observational studies (840 patients) were included in the analysis. 58% of HNAD patients were male (95% CI: 45.2–69.7). Overall prevalence of <i>Malassezia</i>-specific IgE among HNAD patients was 79.3% (95% CI: 57.5–91.5). Prevalence of <i>Malassezia-</i>specific IgE among HNAD patients varied significantly between geographical regions (<i>p</i> = 0.0441), with 88% in non-Asian regions (95% CI: 61.06–97.17) and 54.73% in Asian regions (95% CI: 34.36–73.63). <i>Malassezia</i>-specific IgE prevalence among HNAD patients varied significantly among studies of higher and lower NOS quality score (<i>p</i> = 0.0386), with 95.42% in studies with NOS ≥7 (95% CI: 63.54–99.60) and 58.05% in studies with NOS &lt;7 (95% CI: 41.44–73.01). <i>Malassezia</i>-specific IgE prevalence among HNAD patients did not vary significantly between more and less predominant <i>Malassezia</i> species (<i>p</i> = 0.1048). <i>Malassezia</i> spp. plays a crucial role in the pathogenesis of HNAD, and IgE anti-<i>Malassezia</i> antibodies appeared to be a common marker for HNAD. Understanding the pathophysiology of <i>Malassezia</i> in HNAD can help develop more targeted therapeutic approaches in managing AD.</p>","PeriodicalId":12243,"journal":{"name":"Experimental Dermatology","volume":"33 6","pages":""},"PeriodicalIF":3.6,"publicationDate":"2024-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141295836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of a low-mineralized thermal spring water on skin barrier mechanical properties using atomic force microscopy","authors":"C. Mias,&nbsp;A. Stennevin,&nbsp;G. Doat,&nbsp;A. Catté,&nbsp;J. Chlasta,&nbsp;S. Bessou-Touya,&nbsp;H. Duplan","doi":"10.1111/exd.15113","DOIUrl":"10.1111/exd.15113","url":null,"abstract":"<p>The mineral content of thermal spring water (TSW) applied to the skin surface can directly influence the skin barrier. Indeed, our previous study showed that Avène TSW (ATSW), a low mineral content thermal spring water, protects the stratum corneum from dehydration compared to a mineral-rich TSW (MR-TSW) and maintains skin surface ultrastructure. While many TSWs have been recognized to have beneficial effects on skin, little is known about their localized and specific effects on skin barrier biomechanics at the nanometric scale. The aim of this study was to compare the effects of ATSW with a reference, MR-TSW, on the biomechanical barrier properties of the skin under homeostasis conditions using atomic force microscopy (AFM). AFM was used to obtain a precise nanomechanical mapping of the skin surface after three applications of both TSW. This provides specific information on the skin topographical profile and elasticity. The topographic profile of skin samples showed a specific compaction of the skin layers after application of MR-TSW, characterized by an increase of the total number of external skin layers, compared to non-treated samples. By contrast, ATSW did not modify the skin topographic profile. High-resolution force/volume acquisitions to capture the elastic modulus showed that it was directly correlated with skin rigidity. The elastic modulus strongly and significantly increased after MR-TSW application compared to non-treated skin. By contrast, applications of ATSW did not increase elastic modulus. These data demonstrate that applications of MR-TSW significantly modified skin barrier properties by increasing skin surface layer compaction and skin rigidity. By contrast, ATSW did not modify the topographical profile of skin explants nor induce mechanical stress at the level of the stratum corneum, indicating it does not disrupt the biophysical properties linked to skin surface integrity.</p>","PeriodicalId":12243,"journal":{"name":"Experimental Dermatology","volume":"33 6","pages":""},"PeriodicalIF":3.6,"publicationDate":"2024-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141295834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"IL-4 and IL-13 are not involved in IL-31-induced itch-associated scratching behaviour in mice","authors":"Rana Kawai,&nbsp;Nao Ichimasu,&nbsp;Kazumoto Katagiri","doi":"10.1111/exd.15115","DOIUrl":"10.1111/exd.15115","url":null,"abstract":"<p>Itchy skin or pruritus is a common cutaneous symptom that causes an urge to scratch, and the role of interleukins (IL) in itchy skin has been widely studied. IL-4 and IL-13 are known to induce chronic itch. Similarly, the direct role of IL-31 in inducing itch has been demonstrated in clinical situations such as atopic dermatitis and prurigo nodularis. Moreover, IL-4 receptor α antibodies (dupilumab) and IL-31 receptor A antibodies (nemolizumab) inhibit pruritus. However, the interplay between these ILs in pruritus remains unclear. Therefore, we investigated the reciprocal effects of these cytokines on pruritus in mice. The intradermal administration of IL-31 induced itch-associated scratching behaviour in a dose-dependent manner. Interestingly, the amount of IL-31 and IL-4/IL-13, co-administration or 30 min pre-administration of IL-4/IL-13 and intradermal or intravenous pre-administration of IL-4 did not affect IL-31-induced itch-associated scratching behaviour when it was observed for 30 min, 2 h, 24 h or 48 h. Pre-administration of neutralising antibodies against IL-4 and IL-13 also did not affect IL-31-induced itch-associated scratching behaviour. These results suggest that IL-31 can induce itching independently of IL-4 and IL-13 in vivo.</p>","PeriodicalId":12243,"journal":{"name":"Experimental Dermatology","volume":"33 6","pages":""},"PeriodicalIF":3.6,"publicationDate":"2024-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141295835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Research progress on the mechanism and signalling pathway of ferroptosis and its potential role in dermatosis research","authors":"Min Li,&nbsp;Jian Gong,&nbsp;Qiao Liu,&nbsp;Weiwei Wu","doi":"10.1111/exd.15114","DOIUrl":"10.1111/exd.15114","url":null,"abstract":"<p>Ferroptosis is a novel type of cell death that is dependent on lipid peroxidation and iron accumulation, which distinguishes it from other types of programmed cell death. Current research indicates a significant association between ferroptosis and various pathological conditions, including cancer, neurological disorders, and cardiovascular diseases, albeit with a relatively unexplored role in dermatological afflictions. This paper elaborates on the mechanisms and signalling pathways of ferroptosis, summarizing the recent studies on ferroptosis and its related factors in dermatosis. Our objective is to shed light on novel perspectives and therapeutic strategies for dermatosis, enhancing the understanding of this under-researched area through this comprehensive review.</p>","PeriodicalId":12243,"journal":{"name":"Experimental Dermatology","volume":"33 6","pages":""},"PeriodicalIF":3.6,"publicationDate":"2024-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/exd.15114","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141295837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"STAT3-induced lncRNA GNAS-AS1 accelerates keloid formation by mediating the miR-196a-5p/CXCL12/STAT3 axis in a feedback loop","authors":"Yun Liu,&nbsp;Teng-Xiao Ma,&nbsp;Peng-Fei Fan,&nbsp;Ze Wang,&nbsp;Zhe Wang,&nbsp;Lei Li","doi":"10.1111/exd.15111","DOIUrl":"10.1111/exd.15111","url":null,"abstract":"<p>Keloids are pathological scar tissue resulting from skin trauma or spontaneous formation, often accompanied by itching and pain. Although GNAS antisense RNA 1 (GNAS-AS1) shows abnormal upregulation in keloids, the underlying molecular mechanism is unclear. The levels of genes and proteins in clinical tissues from patients with keloids and human keloid fibroblasts (HKFs) were measured using quantitative reverse transcription PCR, western blot and enzyme-linked immunosorbent assay. The features of HKFs, including proliferation and migration, were evaluated using cell counting kit 8 and a wound healing assay. The colocalization of GNAS-AS1 and miR-196a-5p in HKFs was measured using fluorescence in situ hybridization. The relationships among GNAS-AS1, miR-196a-5p and C-X-C motif chemokine ligand 12 (CXCL12) in samples from patients with keloids were analysed by Pearson correlation analysis. Gene interactions were validated by chromatin immunoprecipitation and luciferase reporter assays. GNAS-AS1 and CXCL12 expression were upregulated and miR-196a-5p expression was downregulated in clinical tissues from patients with keloids. GNAS-AS1 knockdown inhibited proliferation, migration, and extracellular matrix (ECM) accumulation of HKFs, all of which were reversed by miR-196a-5p downregulation. Signal transducer and activator of transcription 3 (STAT3) induced GNAS-AS1 transcription through GNAS-AS1 promoter interaction, and niclosamide, a STAT3 inhibitor, decreased GNAS-AS1 expression. GNAS-AS1 positively regulated CXCL12 by sponging miR-196-5p. Furthermore, CXCL12 knockdown restrained STAT3 phosphorylation in HKFs. Our findings revealed a feedback loop of STAT3/GNAS-AS1/miR-196a-5p/CXCL12/STAT3 that promoted HKF proliferation, migration and ECM accumulation and affected keloid progression.</p>","PeriodicalId":12243,"journal":{"name":"Experimental Dermatology","volume":"33 6","pages":""},"PeriodicalIF":3.6,"publicationDate":"2024-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141260942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}