Experimental Dermatology最新文献

Population-Specific HLA Profiles in Generalised Pustular Psoriasis in Sarawak, Malaysia. 马来西亚沙捞越广泛性脓疱性银屑病人群特异性HLA谱。

IF 3.1 3区医学

Experimental Dermatology Pub Date : 2026-05-01 DOI: 10.1111/exd.70258

Ingrid Pao Lin Ting, Hock Gin Teo, Bee Tee Koay, Norfarhana Khairul-Fahmy, Masita Arip, Norhazlin Mustafa, Min Moon Tang

{"title":"Population-Specific HLA Profiles in Generalised Pustular Psoriasis in Sarawak, Malaysia.","authors":"Ingrid Pao Lin Ting, Hock Gin Teo, Bee Tee Koay, Norfarhana Khairul-Fahmy, Masita Arip, Norhazlin Mustafa, Min Moon Tang","doi":"10.1111/exd.70258","DOIUrl":"https://doi.org/10.1111/exd.70258","url":null,"abstract":"Genetic studies mutations (IL36RN, CARD14 and AP1S3) account for only 28.6% of generalised pustular psoriasis (GPP) leaving much of its pathogenesis remained to be elucidated. Emerging evidence suggests Th17-mediated inflammation, potentially driven by specific HLA class alleles, may underlie dysregulated IL-36 signalling. This study explores the genotypic and phenotypic features of autochthonous GPP in Sarawak. A cross-sectional case-control study was conducted in Sarawak's three main dermatology centres. GPP patients (n = 43) fulfilling ERASPEN criteria between 1997 and June 2024 were included (23 with GPP alone); 20 with concomitant psoriasis vulgaris. HLA genotyping (HLA-A, -B, -C, -DR) was performed via polymerase chain reaction and sequence-specific oligonucleotide probe hybridisation (PCR-SSO) methods at the Institute of Medical Research (IMR). HLA frequencies were compared to 90 Sarawakian controls from Malaysian Stem Cell Registry. Female predominance was noted (1:4.4). Median age at GPP onset was 29 years. Family history of psoriasis was reported in 30.2%, with 16% females developing GPP during pregnancy. Treatment responses: corticosteroids (100%), ciclosporin (82.1%), acitretin (60%) and methotrexate (50%). While biologics were effective in 10 patients. Common alleles included HLA-DRB1*12:02, HLA-A*11:01 and HLA-C*07:02 while HLA-C06:02 was not observed. HLA-A*02:07 and HLA-B*46:01 were observed only in concomitant psoriasis patients. HLA-A*11:02 was seen in patients with ciclosporin non-response, HLA-A*24:02 in those with poorer responses to acitretin and methotrexate and HLA-B*38:02 in psoriatic arthritis. Lower frequencies of HLA-B*35:05 and HLA-C*04:01 were observed among Dayak patients. The absence of HLA-C*06:02 and variation in allele frequencies in this cohort may reflect population-specific patterns but require validation in larger studies.","PeriodicalId":12243,"journal":{"name":"Experimental Dermatology","volume":"35 5","pages":"e70258"},"PeriodicalIF":3.1,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147766885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

HCA2 Receptors in Lymphocytes and Keratinocytes Affect Murine Contact Allergic Inflammation. 淋巴细胞和角化细胞中的HCA2受体影响小鼠接触性过敏性炎症。

IF 3.1 3区医学

Experimental Dermatology Pub Date : 2026-05-01 DOI: 10.1111/exd.70254

Sina Polkownik, Julia Meens, Hagen Lange, Johannes Weil, Bastian Kruse, Anthony Buzzai, Katharina Wulff, Andreas D Braun, Susanne Bonfiatius, Thomas Tüting, Evelyn Gaffal

{"title":"HCA2 Receptors in Lymphocytes and Keratinocytes Affect Murine Contact Allergic Inflammation.","authors":"Sina Polkownik, Julia Meens, Hagen Lange, Johannes Weil, Bastian Kruse, Anthony Buzzai, Katharina Wulff, Andreas D Braun, Susanne Bonfiatius, Thomas Tüting, Evelyn Gaffal","doi":"10.1111/exd.70254","DOIUrl":"https://doi.org/10.1111/exd.70254","url":null,"abstract":"The hydroxycarboxylic acid receptor HCA2 is expressed in keratinocytes and immune cells. In mice, the anti-inflammatory potential of HCA2 receptor signalling in the skin was first described in experimental models of psoriasis-like inflammation and bullous pemphigoid-like epidermolysis bullosa acquisita. We examined contact allergic immune responses to the obligate contact sensitiser DNFB in Hcar2-/- and wild-type C57BL/6 mice. Basal mRNA levels of pro-inflammatory mediators like IFNγ were already increased in the ear tissue of naïve Hcar2-/- animals. After sensitisation and challenge with DNFB, contact allergic ear swelling and infiltration of neutrophils and CD3+ T cells were increased in Hcar2-/- mice. To investigate the impact of HCA2 receptors on T cells, we performed in vitro co-stimulation assays with allergen-loaded dendritic cells and antigen-specific T cells, showing increased proliferation and IFNγ production of Hcar2-/- T lymphocytes. Adoptive transfer of sensitised lymphocytes and experiments with bone marrow chimeric mice indicated that HCAR2 exerts its anti-inflammatory effect in part through radio-resistant, skin-resident cells in the challenge phase. As a potential mechanism, we found that Hcar2-/- keratinocytes produced higher levels of the neutrophil-attracting chemokine CCL8. In summary, we show that HCA2 receptors are functionally expressed in lymphocytes and keratinocytes and participate in the attenuation of contact allergic immune responses. Our data indicate that the dominant anti-inflammatory effect of HCA2 signalling during the elicitation phase resides in radio-resistant, skin-resident cells, whereas effects on lymphocyte activation are likely to be modulatory. The precise contribution of distinct skin-resident cell populations and the role of endogenous ligands driving HCA2 signalling in this context remain unresolved.","PeriodicalId":12243,"journal":{"name":"Experimental Dermatology","volume":"35 5","pages":"e70254"},"PeriodicalIF":3.1,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147766903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Drug Repurposing of Itraconazole Suppresses Angiogenesis and Hyperplasia in Port Wine Stain via Autophagy-Apoptosis Crosstalk. 伊曲康唑药物再利用通过自噬-凋亡串扰抑制葡萄酒染色血管生成和增生。

IF 3.1 3区医学

Experimental Dermatology Pub Date : 2026-05-01 DOI: 10.1111/exd.70260

Lei Wang, Xin Ran, Chunhua Tan, Zeng Tu, Liqiang Gan, Wentao Lin, Peilin Lu, Kaiwen Zhuang, Yuping Ran, Aijun Chen, Shuang Chen

{"title":"Drug Repurposing of Itraconazole Suppresses Angiogenesis and Hyperplasia in Port Wine Stain via Autophagy-Apoptosis Crosstalk.","authors":"Lei Wang, Xin Ran, Chunhua Tan, Zeng Tu, Liqiang Gan, Wentao Lin, Peilin Lu, Kaiwen Zhuang, Yuping Ran, Aijun Chen, Shuang Chen","doi":"10.1111/exd.70260","DOIUrl":"https://doi.org/10.1111/exd.70260","url":null,"abstract":"Port wine stain (PWS) is a common congenital and progressive skin capillary malformation with poor clinical effects, which seriously affects the patients' appearance and social interaction. In the study, we found that itraconazole (ITRA), a common antifungal agent, showed preliminary clinical improvement in patients with PWS in our pilot observational case series; however, the underlying molecular mechanisms are still unclear. PWS pathogenesis is associated with somatic activating GNAQ R183Q mutations that constitutively activate the PI3K/AKT/mTOR pathway, and angiogenesis and fibroblast hyperplasia further play important roles in its occurrence and development. We isolated and cultured primary human umbilical vein endothelial cells (HUVECs) and PWS fibroblasts (PWSFs) by enzymatic digestion and used these cells as our cell models. In vitro, we found that ITRA inhibited proliferation, induced apoptosis, increased autophagosome formation and promoted autophagy flux of HUVECs and PWSFs. Furthermore, we explored the association between apoptosis and ITRA-induced autophagy. We found that ITRA induced autophagy, and inhibition of autophagy enhanced its pro-apoptotic and anti-proliferative effects of ITRA in PWSFs and HUVECs. In addition, ITRA may inhibit migration and angiogenesis of HUVECs by downregulating the VEGF/PI3K/AKT/mTOR signalling pathway. Together, our study presents a preliminary clinical pilot observation and a relative experimental study for the potential effects of ITRA in PWS via autophagy-apoptosis crosstalk.","PeriodicalId":12243,"journal":{"name":"Experimental Dermatology","volume":"35 5","pages":"e70260"},"PeriodicalIF":3.1,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147856195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unravelling the Gut-Skin Axis in Chronic Urticaria: Dysbiosis, Metabolites and Immunological Mechanisms. 慢性荨麻疹肠道-皮肤轴的解开：生态失调、代谢产物和免疫机制。

IF 3.1 3区医学

Experimental Dermatology Pub Date : 2026-05-01 DOI: 10.1111/exd.70261

Yujia Wu, Jipeng Liu, Yunqing Ren, Chaochun Zou

{"title":"Unravelling the Gut-Skin Axis in Chronic Urticaria: Dysbiosis, Metabolites and Immunological Mechanisms.","authors":"Yujia Wu, Jipeng Liu, Yunqing Ren, Chaochun Zou","doi":"10.1111/exd.70261","DOIUrl":"https://doi.org/10.1111/exd.70261","url":null,"abstract":"Although the core pathophysiological pathways of chronic urticaria (CU) are increasingly understood, the upstream triggers and factors contributing to disease chronicity remain poorly understood. Emerging evidence suggests that gut microbiota dysbiosis represents a potentially modifiable upstream factor, which has been predominantly investigated in patients with chronic spontaneous urticaria (CSU). Multi-omics and Mendelian randomization studies have provided convergent evidence linking gut dysbiosis to systemic inflammation and mast cell instability. This is characterized primarily by the depletion of short-chain fatty acid (SCFA)-producing taxa (e.g., Faecalibacterium, Roseburia and Bifidobacterium) and the relative enrichment of pro-inflammatory Proteobacteria (particularly Enterobacteriaceae). Mechanistically, these alterations may lower the mast cell activation threshold and promote systemic immune dysregulation through specific metabolic shifts, such as the depletion of SCFAs and unsaturated fatty acids, and the translocation of endotoxins (e.g., lipopolysaccharide) due to compromised intestinal barrier function. In this review, we discuss how the use of Mendelian randomization (MR) and germ-free mouse models can advance the gut-urticaria axis (with a primary focus on CSU) from mere correlation to causation, while highlighting the crucial need to account for clinical confounders. Finally, we evaluate the clinical translational potential and associated challenges of microbiome-targeted interventions (e.g., probiotics, faecal microbiota transplantation) as novel adjuvant therapies.","PeriodicalId":12243,"journal":{"name":"Experimental Dermatology","volume":"35 5","pages":"e70261"},"PeriodicalIF":3.1,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147835494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Phase II Trial of Perioperative Oral Itraconazole for the Management of Low-Risk Basal Cell Carcinoma. 围手术期口服伊曲康唑治疗低危基底细胞癌的II期临床研究。

IF 3.1 3区医学

Experimental Dermatology Pub Date : 2026-05-01 DOI: 10.1111/exd.70264

Rodrigo Pérez Pereira, Dienifer Hermann Sirena, Sérgio Jobim de Azevedo, Monique Maria Franco da Silva, Mariani Magnus da Luz Andrade, Charles Francisco Ferreira, Tiago Elias Heinen, Renato Marchiori Bakos

{"title":"A Phase II Trial of Perioperative Oral Itraconazole for the Management of Low-Risk Basal Cell Carcinoma.","authors":"Rodrigo Pérez Pereira, Dienifer Hermann Sirena, Sérgio Jobim de Azevedo, Monique Maria Franco da Silva, Mariani Magnus da Luz Andrade, Charles Francisco Ferreira, Tiago Elias Heinen, Renato Marchiori Bakos","doi":"10.1111/exd.70264","DOIUrl":"10.1111/exd.70264","url":null,"abstract":"Recently, new treatment strategies have been developed for advanced or metastatic basal cell carcinoma (BCC), including Hedgehog pathway inhibitors. Itraconazole has demonstrated clinical activity in such cases by blocking the smoothened (SMO) receptor. Such a strategy could be used in early disease. The objective of this Phase II study was to evaluate the clinical and molecular efficacy of Itraconazole in low-risk BCC patients who were primarily candidates for surgical excision. Lesions were assessed according to RECIST 1.1 criteria, with target lesions being at least 10 mm in size after confirmatory biopsy. Patients received itraconazole tablets 200 mg twice daily for 60 days before resection. The median tumour diameter before treatment was 14 mm (IQR 11-16 mm), and after treatment, 13 mm (IQR 11-15 mm), and this reduction in tumour size was statistically significant (p < 0.0001). Coupled with the decrease in tumour size, a decrease in the expression of CD105, an endothelial marker (p < 0.0001), was observed. In conclusion, neoadjuvant itraconazole for 2 months was able to reduce the diameter of low-risk BCC, and the effect was associated with a decrease in tumour angiogenesis and a favourable safety profile.","PeriodicalId":12243,"journal":{"name":"Experimental Dermatology","volume":"35 5","pages":"e70264"},"PeriodicalIF":3.1,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13155031/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147834547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Depth-Dependent Distribution of LYVE-1⁺ Macrophages in Adult Human Skin. 成人皮肤中LYVE-1+巨噬细胞深度依赖性分布

IF 3.1 3区医学

Experimental Dermatology Pub Date : 2026-05-01 DOI: 10.1111/exd.70259

Kazuki Takagaki

引用次数: 0

Use of Baricitinib in Patients With TIF1-γ Positive Dermatomyositis Following a COVID-19 Infection. Baricitinib在COVID-19感染后TIF1-γ阳性皮肌炎患者中的应用

IF 3.1 3区医学

Experimental Dermatology Pub Date : 2026-05-01 DOI: 10.1111/exd.70257

Guan-Kai Lai, Chia-Yu Chu, Yung-Tsu Cho

引用次数: 0

Dermal Fibroblasts Step Into the Spotlight as Potent Amplifiers of Cutaneous Inflammation. 皮肤成纤维细胞成为皮肤炎症的有效放大器。

IF 3.1 3区医学

Experimental Dermatology Pub Date : 2026-05-01 DOI: 10.1111/exd.70263

Tan Nguyen Manh, Yu Sawada

引用次数: 0

Exploring the Associations Between Mediterranean Diet Adherence and Autoinflammation-Associated Skin Diseases. 探索地中海饮食依从性与自身炎症相关皮肤病之间的关系

IF 3.1 3区医学

Experimental Dermatology Pub Date : 2026-05-01 DOI: 10.1111/exd.70247

Krithika Nayudu, Sofia Milosavljevic, Natalie Braun, Vinod Nambudiri

{"title":"Exploring the Associations Between Mediterranean Diet Adherence and Autoinflammation-Associated Skin Diseases.","authors":"Krithika Nayudu, Sofia Milosavljevic, Natalie Braun, Vinod Nambudiri","doi":"10.1111/exd.70247","DOIUrl":"https://doi.org/10.1111/exd.70247","url":null,"abstract":"Autoinflammatory diseases (AIDs) are characterized by abnormal responses of the innate immune system, accompanied by elevations in inflammatory biomarkers, in the absence of a physiologic crisis or infection. Certain monogenic AIDs may present with acneiform or hidradenitis suppurativa-like cutaneous manifestations. In contrast, acne vulgaris, plaque psoriasis and hidradenitis suppurativa are complex, multifactorial inflammatory dermatoses situated along an autoinflammation-associated immunologic spectrum, involving both innate and adaptive immune pathways. There is a growing sphere of research dedicated to exploring dietary patterns as modifiable factors associated with inflammatory disease activity. The Mediterranean diet (MD)-which includes a high consumption of vegetables, whole grains, legumes, fruits, nuts, seeds, fish, wine and extra-virgin olive oil (EVOO)-has gained attention for its potential to modulate pathogenic inflammatory mechanisms. In this review, we summarize the current evidence examining associations between MD adherence and three autoinflammation-associated skin conditions: acne vulgaris, psoriasis and hidradenitis suppurativa. We highlight shared and disease-specific inflammatory pathways while emphasizing the predominance of observational data and the need for further interventional studies to clarify clinical implications.","PeriodicalId":12243,"journal":{"name":"Experimental Dermatology","volume":"35 5","pages":"e70247"},"PeriodicalIF":3.1,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147766924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Secretome of Bullous Pemphigoid IgG-Treated Keratinocytes Induces a Pro-Inflammatory Eosinophil Response. 大疱性类天疱疮igg处理的角质形成细胞分泌组诱导促炎性嗜酸性粒细胞反应。

IF 3.1 3区医学

Experimental Dermatology Pub Date : 2026-05-01 DOI: 10.1111/exd.70265

Adrian P Mansini, Lei Bao, Krishan D Chhiba, Jing Li, Yulu Wang, Haley Gainer, M Allen McAlexander, Christopher McCrae, Christopher D Nazaroff, Fei Li Kuang, Kyle T Amber

{"title":"The Secretome of Bullous Pemphigoid IgG-Treated Keratinocytes Induces a Pro-Inflammatory Eosinophil Response.","authors":"Adrian P Mansini, Lei Bao, Krishan D Chhiba, Jing Li, Yulu Wang, Haley Gainer, M Allen McAlexander, Christopher McCrae, Christopher D Nazaroff, Fei Li Kuang, Kyle T Amber","doi":"10.1111/exd.70265","DOIUrl":"10.1111/exd.70265","url":null,"abstract":"Bullous pemphigoid (BP) is an autoimmune blistering disease whereby the cutaneous antigens BP180 and BP230 are targeted by autoantibodies. Skin lesions in BP are characterized by an abundance of eosinophils. We recently demonstrated that the treatment of keratinocytes with antibodies from patients with BP induces a robust inflammatory response with release of numerous cytokines, chemokines, complement factors, and proteases. We thus questioned whether this keratinocyte inflammatory response was capable of directly inducing an inflammatory response in eosinophils. We therefore treated human eosinophils with conditioned media from keratinocytes treated with IgG from patients with BP (BP-IgG), or healthy controls (control-IgG) with or without supplemental IL-5. Flow cytometry revealed upregulation of CD107a/CD107b, markers of degranulation, on eosinophils treated with supernatants from BP-IgG relative to control-IgG treated keratinocytes. A decrease in CCR3 and CD101 was also identified. Functional activity of eosinophils was confirmed by performing a multiplex immunoassay on eosinophil supernatants. This revealed significant upregulation IL-6, IL-8, LIF, TGFα, MCP-4, MMP-9, and MMP-10. Supplemental IL-5 did not appear to significantly influence these responses. Our data demonstrate that the inflammatory activation of keratinocytes by BP-IgG affects eosinophils, driving phenotypic changes consistent with degranulation, as well as a pro-inflammatory and proteolytic response.","PeriodicalId":12243,"journal":{"name":"Experimental Dermatology","volume":"35 5","pages":"e70265"},"PeriodicalIF":3.1,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13154717/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147835372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0