Experimental Dermatology最新文献

{"title":"Lipase M Is Essential for Skin Barrier Function in Mice","authors":"Hong-Ming Zhou,&nbsp;Jinan Li,&nbsp;Shaohui Liu,&nbsp;Miguel Barriera Diaz,&nbsp;Qun Wang,&nbsp;Emma H. Doud,&nbsp;Whitney Smith-Kinnaman,&nbsp;Derrick A. Gray,&nbsp;Sou Shirai,&nbsp;Keisuke Jojima,&nbsp;Akio Kihara,&nbsp;Bo Zhao,&nbsp;Matthew J. Turner","doi":"10.1111/exd.70133","DOIUrl":"https://doi.org/10.1111/exd.70133","url":null,"abstract":"<p>A functional skin barrier is essential for restricting water losses for terrestrial animals. The outermost layer of this barrier, the stratum corneum, consists of corneocytes (derived from terminally differentiated keratinocytes) coated with a protein-rich envelope and a conjugated lipid-rich envelope embedded in an organised hydrophobic intercellular lipid matrix. Gene mutations and environmental insults that affect corneocyte formation or the intercellular lipid matrix organisation cause skin barrier defects in mice and humans. Here we demonstrate mice homozygous for a loss-of-function mutation in the <i>Lipm</i> gene exhibit a diminished and discontinuous intercellular lipid matrix and a profound skin barrier defect associated with neonatal lethality. These findings suggest the protein encoded by the <i>Lipm</i> gene, lipase M (LIPM), is essential for skin barrier function by impacting intercellular lipid matrix organisation in the stratum corneum.</p>","PeriodicalId":12243,"journal":{"name":"Experimental Dermatology","volume":"34 7","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/exd.70133","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144589520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"New Methodological Approach to Follow the Re-Epithelialisation Phase in the Wound Healing Process on a 3D Full Thickness Skin Model","authors":"Bénédicte Fallou,&nbsp;Mylène Pelleter,&nbsp;Florence Innamorato,&nbsp;Mickaël Roche,&nbsp;Michel Bataillon","doi":"10.1111/exd.70137","DOIUrl":"https://doi.org/10.1111/exd.70137","url":null,"abstract":"<div>\u0000 \u0000 <p>Scratch assays are commonly used as screening tools to assess the skin regenerative potential of new active ingredients; however, they lack the complexity of 3D stratified epidermis. Previously, a 3D migration model was developed to mimic the re-epithelialisation, especially phase III and IV of the wound healing process. To monitor the regenerative process on this model, Papanicolaou staining paired with histological observation at each day of the study was used. Unfortunately, the potential of this 3D model as a screening tool was limited since this methodology is time consuming, and its invasive nature implicates a large number of samples and high variability. An alternative method allowing evaluation of re-epithelialisation in 3D would circumvent these limitations and consolidate the in vitro evaluation model. This study introduces a novel methodology employing non-invasive Optical Coherence Tomography (OCT) and image processing algorithms, combined with a final quantification of the histological quality, to evaluate re-epithelialisation in a 3D skin model. OCT technology showed a high correlation to the previously described Papanicolaou staining technique. Moreover, two pro-epithelialisation compounds, oncostatin M (OSM) and ascorbic acid (VITC), were used as positive controls, bestowing the model with different repair profiles. This approach represents a significant advancement over traditional methods by proposing a reliable and robust evaluation method, extending the capability of the 3D in vitro model as a potential screening tool to understand the mechanisms of action of pro-epithelialisation actives in the process of skin regeneration and healing.</p>\u0000 </div>","PeriodicalId":12243,"journal":{"name":"Experimental Dermatology","volume":"34 7","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144573225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing Early Detection of Metastatic Cutaneous Squamous Cell Carcinoma (CSCC): Integrating AI With Histopathological Assessments","authors":"E. M. Bramer,&nbsp;C. Chia,&nbsp;B. Rentroia-Pacheco,&nbsp;S. Tokez,&nbsp;L. Pijnenborg,&nbsp;J. Damman,&nbsp;A. Amir,&nbsp;D. Kumar,&nbsp;L. M. Hollestein,&nbsp;A. L. Mooyaart,&nbsp;M. Wakkee","doi":"10.1111/exd.70135","DOIUrl":"https://doi.org/10.1111/exd.70135","url":null,"abstract":"<p>Cutaneous squamous cell carcinoma (CSCC) patients at high risk for metastasis are insufficiently identified with current staging systems. Advances in digital pathology and artificial intelligence (AI) might assist by extracting detailed and reproducible predictive features from haematoxylin and eosin slides. We evaluated a multi-step convolutional neural network (CNN) as an assistive tool to provide detailed complementary histopathological variables towards identifying high-risk CSCC. Using a nested case–control design, we studied patients diagnosed with primary CSCC in the Netherlands from 2007 to 2018, with metastatic patients as cases and non-metastatic patients as controls. The dataset was divided into a development set (130 patients) and an evaluation set (244 patients). Four elaborative variables were derived from a CNN model for object detection and semantic segmentation, complementing six dermatopathologist-scored histopathological variables. Dermatopathologists involved were blinded to the outcomes. We assessed the efficacy of these variables using multivariable logistic regression (MR) models and odds ratios (OR) for metastatic CSCC on the evaluation set. The MR model fitting was assessed using the pairwise concordance index (C-index). The combined dermatopathologist-AI model yielded the highest C-index (0.92 [0.87–0.95]). Significant variables in the combined model included model-derived tumour area (OR 1.35 [1.00–1.84]) which complemented scored tumour diameter (OR 1.54 [0.75–3.17]) and model-derived nuclei density (OR 3.14 [1.08–9.17]) as a counterpart of scored tumour differentiation grades (OR 10.6 [3.01–37.0] and 11.5 [2.95–44.5]). The CNN model can derive detailed and reproducible histopathological variables associated with metastatic risk in CSCC, complementing the current pathologist-based assessment and enhancing the identification of high-risk CSCC.</p>","PeriodicalId":12243,"journal":{"name":"Experimental Dermatology","volume":"34 7","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/exd.70135","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144550848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Incidence and Clinical Characteristics of Active Tuberculosis in Psoriasis Patients From a High-Burden Setting: An 18-Year Retrospective Study of 86 Patients","authors":"Suphattra Trakanwittayarak,&nbsp;Leena Chularojanamontri,&nbsp;Chayada Chaiyabutre,&nbsp;Narumol Silpa-archa,&nbsp;Chanisada Wongpraparut,&nbsp;Praveena Chiowchanwisawakit","doi":"10.1111/exd.70134","DOIUrl":"https://doi.org/10.1111/exd.70134","url":null,"abstract":"<p>Real-world data on concurrent psoriasis and active tuberculosis (TB) remain limited, particularly in high TB-burden settings. This retrospective study evaluated the incidence, prevalence, and clinical characteristics of psoriasis patients with active TB who had received topical or systemic treatments. Medical records from 13 066 psoriasis patients who presented at Siriraj Hospital over 18 years were reviewed. Among these, 86 (0.66%) developed active TB, yielding an incidence range of 135–1332 per 100 000 psoriasis patients. The mean patient age was 50.4 <b>±</b> 15.7 years; 63 were men and 23 were women. Pulmonary TB occurred in 55 patients (64.0%), whereas 31 (36.0%) developed extrapulmonary TB. Male sex and smoking were associated with pulmonary TB. The most common pulmonary symptoms were chronic cough (65.5%) and dyspnoea (60.0%), although 7.3% were asymptomatic. Time to TB onset was shorter for extrapulmonary cases (5.7 <b>±</b> 5.1 years) than for pulmonary cases (7.4 <b>±</b> 6.5 years), but this difference was not statistically significant. Extrapulmonary disease most frequently involved the lymph node and pleura (25.8%) or the gastrointestinal tract (16.1%). Notably, all four patients who received infliximab within 1 year before TB diagnosis developed extrapulmonary TB. In conclusion, the incidence of TB in psoriasis patients in endemic regions may be high. Geographic factors, sex, smoking, and treatment history appear to influence TB risk. Close monitoring is critical, particularly in high-burden settings.</p>","PeriodicalId":12243,"journal":{"name":"Experimental Dermatology","volume":"34 7","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/exd.70134","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144519931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Humanised Mice in Cutaneous Leishmaniasis—T-Cell Recruitment Into Human Skin Transplants After Leishmania major Infection","authors":"Ling Miao,&nbsp;Henning Klapproth,&nbsp;Michael R. Stepkes,&nbsp;Joanna Wegner,&nbsp;Esther von Stebut","doi":"10.1111/exd.70131","DOIUrl":"https://doi.org/10.1111/exd.70131","url":null,"abstract":"<p>Treatment against leishmaniasis is associated with severe side effects, high costs, and parasitic resistance. Preclinical models such as humanised mice would aid therapeutic improvement or the development of a vaccine. We developed a model in which human skin transplants on immunodeficient mice are infected with <i>Leishmania major</i>. Parasite inoculation of the skin transplant led to a robust infection with increasing numbers of parasites in the skin and visceral organs. In addition, intraperitoneally co-administered allogeneic peripheral blood mononuclear cells (PBMCs) were strongly recruited to skin lesions, with ≥ 65% of the cells being positive for anti-human CD45; we identified ~20% CD4⁺ and ~50% CD8⁺ human T cells. The number of skin-resident macrophages or dendritic cells was unaltered compared to healthy skin prior to transplantation, and PBMC administration did not alter their numbers. Together, we show that parasitic infection provides a strong inflammatory signal that leads to recruitment of T cells into skin transplants. The presence of antigen-presenting cells in the transplants—as an important prerequisite for proper APC-T-cell interaction—recreates a fully human skin microenvironment that allows for stroma/immune cell interactions upon infection. This model may be of high interest to researchers interested in translating skin research questions into the human system in vivo.</p>","PeriodicalId":12243,"journal":{"name":"Experimental Dermatology","volume":"34 7","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/exd.70131","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144519930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Precision at the Cutting Edge: Ex Vivo Confocal Microscopy for Perioperative Tumour Thickness Assessment in Melanoma","authors":"D. Hartmann,&nbsp;A. Swarlik,&nbsp;L. Buttgereit,&nbsp;L. Stärr,&nbsp;K. Kerl-French,&nbsp;M. Flaig,&nbsp;E. C. Sattler,&nbsp;L. E. French,&nbsp;M. Deußing","doi":"10.1111/exd.70136","DOIUrl":"https://doi.org/10.1111/exd.70136","url":null,"abstract":"<p>Ex vivo confocal laser microscopy (EVCM) represents a promising diagnostic tool for the immediate assessment of fresh tissue, with significant potential for the management of melanoma. This study aimed to evaluate the accuracy of EVCM in determining perioperative tumour thickness, a critical factor in guiding treatment strategies for melanoma. A total of 27 confirmed melanomas of varying thickness and from multiple anatomic sites were analysed using both EVCM and gold standard conventional histopathology. Tumour thickness was independently measured using confocal tumour thickness (CTT) and histopathological tumour thickness (HTT) and subsequently compared using correlation analysis, Spearman's correlation coefficient and Bland–Altman plot. Our findings demonstrate a high correlation between HTT and CTT, with a Spearman's correlation coefficient of 0.94. Bland–Altman analysis revealed a mean difference of −0.19 ± 0.72 mm between CTT and HTT, indicating a strong agreement between the two measurement methods. These results underscore the potential of EVCM as a reliable tool for perioperative evaluation of tumour thickness in melanoma, potentially streamlining the decision-making process for surgical margins and improving patient outcomes. Further studies with larger sample sizes are warranted to validate these findings and explore the broader applicability of EVCM in clinical practice.</p>","PeriodicalId":12243,"journal":{"name":"Experimental Dermatology","volume":"34 7","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/exd.70136","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144519916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Staphylococcus aureus Promotes Cutaneous Lesions in Patients With Epidermolysis Bullosa","authors":"Qin Zeng,&nbsp;Shucui Wang,&nbsp;Nadira Nurxat,&nbsp;Xuemeng Min,&nbsp;Yanan Guo,&nbsp;Fuying Chen,&nbsp;Weitao Tang,&nbsp;Yali Yang,&nbsp;Qian Liu,&nbsp;Ming Li","doi":"10.1111/exd.70129","DOIUrl":"https://doi.org/10.1111/exd.70129","url":null,"abstract":"<div>\u0000 \u0000 <p>Epidermolysis bullosa (EB) is a group of rare, heterogeneous congenital conditions characterised by epidermal fragility, resulting in blister formation and lesions. Patients with EB are prone to developing cutaneous wounds. However, the composition of the EB skin microbiome in Chinese individuals remains poorly understood. The objective was to investigate the EB skin microbiome in Chinese individuals. The clinical symptoms and laboratory tests were collected for a total of 29 EB patients (23 Recessive Dystrophic EB, 3 EB simplex, 2 Kindler syndrome, and 1 Dominant Dystrophic EB). A total of 120 swabs were collected from 62 lesion sites, 29 non-lesion skin areas, and 29 nostrils. These samples underwent 16S rRNA amplicon sequencing and bacterial culture. The epidemiology of <i>S. aureus</i> was characterised, and its features were analysed using an animal model. Patients with EB exhibited a characteristic inflammatory response, marked by cutaneous lesions and elevated levels of <i>C</i>-reactive protein (CRP) and serum amyloid (SAA). Consistently, skin dysbiosis in EB patients was characterised by a predominance of <i>S. aureus</i>, particularly sequence type (ST) 7. Specifically, the abundance of <i>S. aureus</i> showed a positive correlation with EB severity and activity. Mechanistically, <i>S. aureus</i> isolated from lesional skin exhibited higher virulence due to increased accessory gene regulator (Agr) activity. Our study reported altered bacterial diversity and increased carriage of higher-virulence <i>S. aureus</i> in Chinese EB patients, which may potentially influence disease severity through microbiome alterations. Our findings suggested that maintaining the balance of the microbiome is crucial for optimising patient care.</p>\u0000 </div>","PeriodicalId":12243,"journal":{"name":"Experimental Dermatology","volume":"34 6","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144473037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The First Reported Japanese Case of PNPLA1-Nonsyndromic Epidermal Differentiation Disorder (PNPLA1-nEDD) Associated With an Unreported 92-Base-Pair Duplication Variant","authors":"Shiori Kato,&nbsp;Takuya Takeichi,&nbsp;Keisuke Jojima,&nbsp;Maiko Kato,&nbsp;Michiya Omi,&nbsp;Kana Tanahashi,&nbsp;Atsushi Otsuka,&nbsp;Yoshinao Muro,&nbsp;Akio Kihara,&nbsp;Masashi Akiyama","doi":"10.1111/exd.70130","DOIUrl":"https://doi.org/10.1111/exd.70130","url":null,"abstract":"<div>\u0000 \u0000 <p>Autosomal recessive congenital ichthyosis (ARCI) is a rare inherited skin disorder marked by generalised scaling and erythema. Very recently, diseases formerly diagnosed as ichthyosis, including ARCI, have been renamed nonsyndromic epidermal differentiation disorders (nEDDs). Although pathogenic variants in various genes, including <i>PNPLA1</i>, have been identified as causes, regional and ethnic differences exist in their prevalence. <i>PNPLA1</i> encodes a unique transacylase essential for acylceramide synthesis, a critical component of the skin barrier. Here, we describe the first reported Japanese case of a <i>PNPLA1</i>-related nonsyndromic epidermal differentiation disorder (<i>PNPLA1</i>-nEDD) caused by a homozygous 92-base-pair duplication variant (c.36_127dup, p.Leu43Profs*46) in <i>PNPLA1</i>. Lipidomic analysis of the stratum corneum revealed marked reductions in ω-<i>O</i>-acylceramides and protein-bound ceramides, alongside the accumulation of non-acylated ceramides—indicating a failure in acylceramide synthesis. This ceramide profile supports the conclusion that the <i>PNPLA1</i> duplication causes a loss of function. Our findings emphasise the importance of <i>PNPLA1</i> in the genetic screening of nEDD cases in Japan, regardless of clinical subtype, due to the absence of genotype–phenotype correlations and to broad phenotypic variability.</p>\u0000 </div>","PeriodicalId":12243,"journal":{"name":"Experimental Dermatology","volume":"34 6","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144339673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Psychological Stress Overactivates IL-23/Th17 Inflammatory Axis and Increases cDC2 in Imiquimod-Induced Psoriasis Models of C57BL/6 Mice","authors":"Jue Tang,&nbsp;Siqi Zhao,&nbsp;Yanqian Su,&nbsp;Huijuan Shi,&nbsp;Xuan Li,&nbsp;Xuan Zhang,&nbsp;Jing Wang,&nbsp;Xinping Jin,&nbsp;Yanling He","doi":"10.1111/exd.70128","DOIUrl":"https://doi.org/10.1111/exd.70128","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Psychological stress often accompanies psoriasis, and both conditions involve an overactive IL-23/Th17 inflammatory axis. However, the mechanism behind the comorbidity of psoriasis and psychological stress remains unclear. This study established a mouse model of comorbid psoriasis and psychological stress to investigate the impact on skin inflammation and the IL-23/Th17 axis. Chronic restraint stress and imiquimod cream were used to induce psychological stress and psoriasis. Behavioural tests included the open-field test and elevated plus maze, while psoriasis severity was assessed using the Psoriasis Severity Index and histopathology. Flow cytometry measured cDC2 populations in the lesion and spleen, and ELISA quantified levels of key inflammatory factors in serum and lesions. Mice with psoriasis alone showed anxiety symptoms, which were significantly exacerbated in the comorbid group. The comorbid group exhibited more severe lesions, with elevated levels of IL-23 and IL-17A in both serum and lesions compared to the psoriasis-only group. The number of CD11c⁺ cDCs in the dermis and the proportion of cDC2s in the lesion and spleen were also significantly higher in the comorbid group. Psychological stress intensifies anxiety symptoms and exacerbates the inflammatory response in the skin and systemic tissues of mice with comorbid psoriasis and psychological stress. This occurs via the overactivation of the IL-23/Th17 inflammatory axis, leading to increased mobilisation of cDC2s and higher levels of inflammatory mediators like IL-23 and IL-17A, thus chronicling inflammation. These findings enhance our understanding of the mechanisms linking psoriasis and psychological disorders, paving the way for identifying potential therapeutic targets.</p>\u0000 </section>\u0000 </div>","PeriodicalId":12243,"journal":{"name":"Experimental Dermatology","volume":"34 6","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144323514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Editorial: New Target for Skin Cancer","authors":"Motoki Nakamura,&nbsp;Dai Ogata","doi":"10.1111/exd.70127","DOIUrl":"https://doi.org/10.1111/exd.70127","url":null,"abstract":"<div>\u0000 \u0000 <p>Skin cancer encompasses a diverse spectrum of malignancies with increasing global incidence and persistent clinical challenges. Despite advances in therapies such as immune checkpoint inhibitors and targeted agents, many patients—especially those with rare subtypes or immunologically ‘cold’ tumours—face limited options and poor outcomes. This special issue of Experimental Dermatology, titled ‘New Target for Skin Cancer’, highlights 29 original articles that reflect the evolving landscape of dermatologic oncology. The collection spans six key themes: molecular and genetic mechanisms, tumour microenvironment and immunology, novel diagnostic biomarkers, emerging therapeutic strategies, rare cancer subtypes and technological innovations. Studies presented include identification of small nucleolar RNAs and metabolic pathways as prognostic markers, analysis of tertiary lymphoid structures and the use of spatial omics and artificial intelligence for diagnostic refinement. Promising therapeutic strategies discussed involve antibody-drug conjugates, oncolytic viruses and stromal-targeting agents. Several articles focus on underrepresented cancers such as extramammary Paget's disease and dermatofibrosarcoma protuberans, underscoring the need for more inclusive research. Technological advances such as ex vivo functional drug screening and mobile health platforms are also explored as tools to personalise and expand access to care. Collectively, these contributions illustrate how interdisciplinary integration of molecular biology, immunology, engineering and AI is reshaping both research and clinical practice in skin cancer. This issue serves as both a comprehensive update on current progress and a forward-looking roadmap for future innovations aimed at improving patient outcomes across all skin cancer types.</p>\u0000 </div>","PeriodicalId":12243,"journal":{"name":"Experimental Dermatology","volume":"34 6","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144299886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}