FEBS Open Bio最新文献_第3页

The role of circular RNAs in regulating cytokine signaling in cancer. 环状rna在调节肿瘤细胞因子信号传导中的作用。

IF 2.8 4区生物学

FEBS Open Bio Pub Date : 2025-05-12 DOI: 10.1002/2211-5463.70051

Vandana Joshi, Swati, Amit Mishra, Amaresh Panda, Vivek Sharma

引用次数: 0

Iron-dependent lysosomal LDL oxidation induces the expression of scavenger receptor A in human THP-1 monocytes. 铁依赖性溶酶体LDL氧化诱导人THP-1单核细胞清道夫受体A的表达。

IF 2.8 4区生物学

FEBS Open Bio Pub Date : 2025-05-11 DOI: 10.1002/2211-5463.70048

Martina Čierna, Richard Buchal, Martin Leníček, Amit Shachak, Jan Pláteník

{"title":"Iron-dependent lysosomal LDL oxidation induces the expression of scavenger receptor A in human THP-1 monocytes.","authors":"Martina Čierna, Richard Buchal, Martin Leníček, Amit Shachak, Jan Pláteník","doi":"10.1002/2211-5463.70048","DOIUrl":"https://doi.org/10.1002/2211-5463.70048","url":null,"abstract":"Atherosclerosis leading to cardiovascular diseases remains a dominant medical problem. In the early stages of this disease, the interaction between circulating monocytes and the endothelium is crucial. Monocytes and macrophages express scavenger receptor A (SR-A), which mediates cell adhesion and subsequently uptake of oxidized low-density lipoproteins (LDL). High iron stores in monocytes or macrophages are known to predispose individuals to atherosclerosis, however the reasons remain poorly understood. We hypothesized that a combination of iron and LDL may induce proatherogenic changes in circulating monocytes. Here, we treated a human monocytic cell line THP-1 with isolated LDL and/or iron. A limited uptake of native LDL, but not iron or oxidized LDL, markedly induced expression of SR-A in these cells. Both SR-AI and SR-AII isoforms were upregulated. The increased SR-A was also seen at the protein level, and LDL treatment increased cellular adhesion. The induction of SR-A by LDL was inhibited by the lysosomotropic thiol WR-1065 and by the chain-breaking lipophilic antioxidant butylated hydroxytoluene (BHT). The fluorescent probe BODIPY C11 exhibited increased lipid peroxidation inside lysosomes after LDL administration. The induction of SR-A by LDL was blocked by two silencing RNAs directed against the nuclear coactivator receptor NCOA4, the cargo receptor necessary for the autophagy of ferritin. These results may point to a new pathogenetic mechanism of early-stage atherosclerosis, in which high iron stores in circulating monocytes, through increased lysosomal lipid peroxidation, may lead to an upregulated expression of SR-A, which makes the cells more adhesive and hence more atherogenic.","PeriodicalId":12187,"journal":{"name":"FEBS Open Bio","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143992284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An approach for coherent periodogram averaging of tilt-series data for improved contrast transfer function estimation. 倾斜序列数据相干周期图平均的改进对比度传递函数估计方法。

IF 2.8 4区生物学

FEBS Open Bio Pub Date : 2025-05-08 DOI: 10.1002/2211-5463.70050

Sagar Khavnekar, William Wan

{"title":"An approach for coherent periodogram averaging of tilt-series data for improved contrast transfer function estimation.","authors":"Sagar Khavnekar, William Wan","doi":"10.1002/2211-5463.70050","DOIUrl":"https://doi.org/10.1002/2211-5463.70050","url":null,"abstract":"Cryo-electron microscopy (cryo-EM) has become an indispensable technique for determining three-dimensional structures of biological macromolecules. A critical aspect of achieving high-resolution cryo-EM reconstructions is accurately determining and correcting for the microscope's contrast transfer function (CTF). The CTF introduces defocus-dependent distortions during imaging; if not properly accounted for, the CTF can distort features in and limit the resolution of 3D reconstructions. For tilt-series data used in cryo-electron tomography (cryo-ET), CTF estimation becomes even more challenging due to the tilt of the specimen, which introduces a defocus gradient across the field of view, as well as the low dose and signal in individual tilt images. Here, we describe a simple algorithm to improve the accuracy of CTF estimation of tilted images by leveraging the tilt-series alignment parameters determined for tomographic reconstruction to explicitly account for the tilted specimen geometry. In brief, each tilt image is divided into patches, each of which are then stretched according to their defocus shift. These are then summed to provide a coherent power spectrum at the tilt axis, which can then be used in standard CTF estimation algorithms. This uses all the data in each image to enhance the visibility of Thon rings, thereby improving high-resolution CTF estimation and subsequent enhancements in the resolution of subtomogram averages.","PeriodicalId":12187,"journal":{"name":"FEBS Open Bio","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143990795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The impact of frailty syndrome on skeletal muscle histology: preventive effects of exercise. 虚弱综合征对骨骼肌组织学的影响：运动的预防作用。

IF 2.8 4区生物学

FEBS Open Bio Pub Date : 2025-05-05 DOI: 10.1002/2211-5463.70049

Fujue Ji, Hae Sung Lee, Haesung Lee, Jong-Hee Kim

{"title":"The impact of frailty syndrome on skeletal muscle histology: preventive effects of exercise.","authors":"Fujue Ji, Hae Sung Lee, Haesung Lee, Jong-Hee Kim","doi":"10.1002/2211-5463.70049","DOIUrl":"https://doi.org/10.1002/2211-5463.70049","url":null,"abstract":"Frailty syndrome, a condition marked by increased vulnerability due to age-related physiological decline, exerts a profound impact on skeletal muscle structure and function. Despite its widespread prevalence, the underlying mechanisms contributing to frailty-associated muscle deterioration remain poorly elucidated. This study utilized histological and biochemical analyses in a murine model to investigate the effects of frailty syndrome on skeletal muscle. Mice were classified based on age and condition, including a subset subjected to an exercise intervention. Parameters evaluated included body weight, lean mass ratio, myofiber size and number, extracellular matrix (ECM) content, and myosin heavy chain isoform expression. Frailty syndrome led to increased body weight and ECM content, coupled with reductions in myofiber size and number, reflecting substantial structural and functional impairments in skeletal muscle. Exercise interventions effectively countered these deleterious changes, preserving myofiber morphology and reducing ECM expansion, thereby demonstrating the protective role of exercise in mitigating frailty-induced muscle deterioration. The study highlights the severe impact of frailty syndrome on skeletal muscle structure and integrity. Importantly, it underscores the potential of regular exercise as an effective therapeutic approach to prevent or reverse muscle deterioration associated with frailty, offering critical insights into managing age-related muscular degeneration.","PeriodicalId":12187,"journal":{"name":"FEBS Open Bio","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143958487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Neurotoxic amyloid β-peptide and tau produce cytokine-like effects on PMCA in glioblastoma cell lines, enhancing its activity and isoforms expression. 神经毒性淀粉样蛋白β-肽和tau对胶质母细胞瘤细胞系PMCA产生细胞因子样作用，增强其活性和同工型的表达。

IF 2.8 4区生物学

FEBS Open Bio Pub Date : 2025-05-05 DOI: 10.1002/2211-5463.70046

María Berrocal, Alberto Alvarez-Barrientos, Ana M Mata

引用次数: 0

Correction to “Characterization of the cHS4 insulator in mouse embryonic stem cells” 更正“小鼠胚胎干细胞中cHS4绝缘体的特性”

IF 2.8 4区生物学

FEBS Open Bio Pub Date : 2025-05-05 DOI: 10.1002/2211-5463.70012

引用次数: 0

An open chat with…Pierre Santucci: a testimonial on why we should engage with scientific societies and their journals 与皮埃尔·桑图奇（Pierre Santucci）的公开谈话：关于我们为什么应该与科学协会及其期刊合作的证明。

IF 2.8 4区生物学

FEBS Open Bio Pub Date : 2025-04-25 DOI: 10.1002/2211-5463.70045

Pierre Santucci, Ioannis Tsagakis

{"title":"An open chat with…Pierre Santucci: a testimonial on why we should engage with scientific societies and their journals","authors":"Pierre Santucci, Ioannis Tsagakis","doi":"10.1002/2211-5463.70045","DOIUrl":"10.1002/2211-5463.70045","url":null,"abstract":"Scientific societies constitute a cornerstone in our scientific landscape by supporting research activities, fostering networking opportunities and promoting extensive training and education in basic sciences. As membership-based organisations, scientific societies provide their members with access to meetings, workshops, educational programs and also funding opportunities through fellowships and awards. Societies also run and supervise the publishing activities of their sponsored journals that benefit authors and the entire community by enabling the publication of high-quality studies by scientists, for scientists. Most societies or not-for-profit publishing organisations financially lean on memberships and their journals to support their activities. Therefore, engaging with and advocating for scientific societies and not-for-profit journals is important to help sustain their impactful activities and to raise awareness for fellow unaware scientists. In this piece, we discuss with our newly appointed Publishing Liaison Officer, Pierre Santucci, how scientific societies and sponsored journals can have a direct and beneficial impact on a scientist's career. Pierre also provides a short testimonial of his experience with FEBS initiatives and FEBS Press journals.","PeriodicalId":12187,"journal":{"name":"FEBS Open Bio","volume":"15 6","pages":"894-897"},"PeriodicalIF":2.8,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/2211-5463.70045","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143960538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to “The first crystal structure of a family 45 glycosidehydrolase from a brown-rot fungus, Gloeophyllum trabeum GtCel45A” 修正“褐腐菌Gloeophyllum trabeum GtCel45A的45家族糖苷水解酶的第一个晶体结构”。

IF 2.8 4区生物学

FEBS Open Bio Pub Date : 2025-04-21 DOI: 10.1002/2211-5463.70040

{"title":"Correction to “The first crystal structure of a family 45 glycosidehydrolase from a brown-rot fungus, Gloeophyllum trabeum GtCel45A”","authors":"","doi":"10.1002/2211-5463.70040","DOIUrl":"10.1002/2211-5463.70040","url":null,"abstract":"\u0000 Laura Okmane, Louise Fitkin, Mats Sandgren, Jerry Ståhlberg. The first crystal structure of a family 45 glycosidehydrolase from a brown-rot fungus, Gloeophyllum trabeum GtCel45A. FEBS Openbio. 2024; 14: 505-514. 10.1002/2211-5463.13774\u0000 The authors include the following sentence in the acknowledgements section:“We acknowledge the European Synchrotron Radiation Facility for provision of synchrotron radiation facilities and we would like to thank the staff of the ESRF and EMBL Grenoble for assistance and support in using beamline ID30B.”The acknowledgements section has been updated as:We thank Dr. Fernando Segato, University of São Paulo, Brazil, for providing spores of the GtCel45A expressing A. nidulans strain. This work was supported by the Swedish Research Council for Environment, Agricultural Sciences and Spatial Planning (Formas), Grant Number 2017–01130. We acknowledge the European Synchrotron Radiation Facility for provision of synchrotron radiation facilities and we would like to thank the staff of the ESRF and EMBL Grenoble for assistance and support in using beamline ID30B.","PeriodicalId":12187,"journal":{"name":"FEBS Open Bio","volume":"15 6","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/2211-5463.70040","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144003847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Matrigel inhibits elongation and drives endoderm differentiation in aggregates of mouse embryonic stem cells. 基质抑制伸长和驱动内胚层分化聚集的小鼠胚胎干细胞。

IF 2.8 4区生物学

FEBS Open Bio Pub Date : 2025-04-19 DOI: 10.1002/2211-5463.70044

Atoosa Amel, Rachel Brown, Alexa Rabeling, Mubeen Goolam

{"title":"Matrigel inhibits elongation and drives endoderm differentiation in aggregates of mouse embryonic stem cells.","authors":"Atoosa Amel, Rachel Brown, Alexa Rabeling, Mubeen Goolam","doi":"10.1002/2211-5463.70044","DOIUrl":"https://doi.org/10.1002/2211-5463.70044","url":null,"abstract":"Modelling peri-implantation mammalian development using the self-organising properties of stem cells is a rapidly growing field that has advanced our understanding of cell fate decisions occurring in the early embryo. Matrigel, a basement membrane matrix, is a critical substrate used in various protocols for its efficacy in promoting stem cell growth and self-organisation. However, its role in driving stem cell lineage commitment, and whether this effect is driven by biochemical or physical cues, is not currently clear. Here, we grow embryoid bodies in suspension, Matrigel and agarose, an inert polysaccharide, to attempt to decouple the physical and biochemical roles of Matrigel and better understand how it drives stem cell differentiation. We use a combination of light microscopy, quantitative PCR and immunostaining to investigate gene and protein changes in our different culture conditions. We show that stem cell aggregates in Matrigel are hindered in their ability to elongate compared with those grown in agarose or in suspension, indicating that prohibitive role in self-organisation. Aggregates in Matrigel are also driven to differentiate into endoderm, with ectoderm differentiation inhibited. Furthermore, these effects are not due to the physical presence of Matrigel, as the same effects are not witnessed in aggregates grown in agarose. Our results thus indicate that Matrigel has a significant and complex effect on the differentiation and morphology of embryoid bodies.","PeriodicalId":12187,"journal":{"name":"FEBS Open Bio","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143958483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Downregulation of O-GlcNAcylation enhances etoposide-induced p53-mediated apoptosis in HepG2 human liver cancer cells. 下调o - glcn酰化可增强依托泊苷诱导的p53介导的HepG2人肝癌细胞凋亡。

IF 2.8 4区生物学

FEBS Open Bio Pub Date : 2025-04-16 DOI: 10.1002/2211-5463.70028

Jaehoon Lee, Gi-Bang Koo, Jihye Park, Byung-Cheol Han, Mijin Kwon, Seung-Ho Lee

{"title":"Downregulation of O-GlcNAcylation enhances etoposide-induced p53-mediated apoptosis in HepG2 human liver cancer cells.","authors":"Jaehoon Lee, Gi-Bang Koo, Jihye Park, Byung-Cheol Han, Mijin Kwon, Seung-Ho Lee","doi":"10.1002/2211-5463.70028","DOIUrl":"https://doi.org/10.1002/2211-5463.70028","url":null,"abstract":"Etoposide, an anticancer drug that inhibits topoisomerase II, is commonly used in combination chemotherapy. However, the impact of O-GlcNAcylation regulation on etoposide's anticancer effects has rarely been investigated. This study evaluated the effect of etoposide on cellular O-GlcNAcylation and whether modulating this process enhances etoposide-induced apoptosis. O-GlcNAc expression was measured after 24 h of etoposide treatment, and the effect of O-GlcNAc transferase (OGT) inhibition by OSMI-1 on etoposide's anticancer activity in HepG2 human liver cancer cells was quantitatively analyzed. Additionally, molecular analyses were used to confirm that the observed effects were mediated by p53-induced apoptosis. Etoposide reduced O-GlcNAcylation in a dose-dependent manner without directly interacting with OGT. Cotreatment with 20 μm of OSMI-1 lowered the IC50 value for cell viability by approximately 1.64-fold to 60.68 μm and increased the EC50 value for cytotoxicity by around 4.07-fold to 43.41 μm. Furthermore, this synergistic effect was linked to the activation of the p53/caspase-3/PARP1 pathway. These findings suggest that downregulating O-GlcNAcylation may enhance the efficacy of etoposide-based chemotherapy and help overcome tumor resistance.","PeriodicalId":12187,"journal":{"name":"FEBS Open Bio","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143958480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0