FEBS Open Bio最新文献

{"title":"Establishment and optimization of the two-step induction system for generating primordial germ cell-like cells from chicken embryonic stem cells.","authors":"Zeyu Li, XianShuai Xu, GuangZheng Liu, XiaoQian Lv, JiuZhou Song, HongYan Sun, YingJie Niu, QiSheng Zuo, Wei Han, BiChun Li, Kai Jin","doi":"10.1002/2211-5463.70116","DOIUrl":"https://doi.org/10.1002/2211-5463.70116","url":null,"abstract":"<p><p>Primordial germ cells (PGCs) are the progenitor cells of sperm and eggs. Xenotransplantation of chicken PGCs can achieve germline transmission. However, there are still challenges in obtaining many PGCs from endangered birds in vitro. In this study, at first, by incorporating 2i factors, the embryonic stem cells (ESCs) culture conditions were optimized, successfully yielding and validating pluripotent ESCs clones. Then, during induction ESCs, bFGF, activin A, and 1% KSR were added to Epiblast-like cells (EpiLCs). Quantitative real-time polymerase chain reaction (qRT-PCR) showed Pax6, Eomes, and Vimentin expression patterns similar to primary epiblast, indicating successful EpiLCs induction. During EpiLCs to Primordial germ cell-like cells (PGCLCs) transformation, we evaluated BMP4, BMP8b, EGF, LIF, and SCF combinations' impact on induction efficiency. Flow cytometry, qRT-PCR, and immunofluorescence showed high expression of Cvh, C-kit, Dazl, CVH, and DAZL in PGCLCs, suggesting successful EpiLCs differentiation. Induced PGCLCs injected into 2.5-day chick embryos migrated to gonads by day 7-7.5, demonstrating migration and colonization. This study optimized a two-step protocol for in vitro differentiation of chicken ESCs into PGCLCs. This research's results not only provide a reference for obtaining many PGCLCs in vitro but also open up a new approach for the development and application of genetic resource preservation technology in domestic chickens.</p>","PeriodicalId":12187,"journal":{"name":"FEBS Open Bio","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145029290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electrical pulse stimulation reflecting the episodic nature of real-life exercise modulates metabolic and secretory profile of primary human myotubes.","authors":"Klára Gabrišová, Tímea Kurdiová, Daria Barkova, Natália Pálešová, Jana Babulicová, Silvia Tyčiaková, Marta Novotová, Mária Balážová, Miroslav Sabo, Václav Pustka, Jozef Ukropec, Barbara Ukropcová","doi":"10.1002/2211-5463.70114","DOIUrl":"https://doi.org/10.1002/2211-5463.70114","url":null,"abstract":"<p><p>Electrical pulse stimulation (EPS) represents a useful tool to study exercise-related adaptations of muscle cells in vitro. Here, we examine the metabolic and secretory response of primary human muscle cells from metabolically healthy individuals to the EPS protocol reflecting the episodic nature of real-life exercise training. This intermittent EPS protocol alternates high-frequency stimulation periods with low-frequency resting periods. Continuous EPS was used as a comparator. Radiometric assessment of glucose and fatty acid metabolism was complemented by examination of mitochondrial OxPHOS proteins, fiber-type markers, and the release of selected myokines and extracellular vesicles into the media. Both EPS protocols facilitated glycogen synthesis and incomplete fatty acid oxidation (intermediary metabolites accumulation), while complete glucose and fatty acid oxidation (CO₂ production) was increased only after the intermittent stimulation. Continuous stimulation elicited robust release of the contraction-regulated myokines (IL6, IL8) into the media. Both EPS protocols increased expression of oxidative fiber-type markers (MYH2, MYH7), while inducing protein expression of a putative myokine, growth differentiation factor11 (GDF11) and a release of extracellular vesicles into the media. In conclusion, intermittent electrical pulse stimulation enhanced the rate of complete glucose and fatty acid oxidation in differentiated muscle cells from metabolically healthy individuals, while it was comparable to continuous stimulation in modulating markers of oxidative fibers and a putative myokine GDF11, and less effective in stimulating the release of myokines IL6, IL8, and extracellular vesicles into the media. Intermittent EPS-a protocol mimicking the episodic nature of exercise-can be used for studying metabolism and the secretome of skeletal muscle cells in vitro.</p>","PeriodicalId":12187,"journal":{"name":"FEBS Open Bio","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145023080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rethinking plastic waste: innovations in enzymatic breakdown of oil-based polyesters and bioplastics.","authors":"Elena Rosini, Nicolò Antonelli, Gianluca Molla","doi":"10.1002/2211-5463.70120","DOIUrl":"https://doi.org/10.1002/2211-5463.70120","url":null,"abstract":"<p><p>The global accumulation of plastic waste, exceeding 360 million tonnes annually, represents a critical environmental challenge due to their widespread use and extreme recalcitrance in natural environments. Furthermore, the end-of-life processing of bioplastics, which are often marketed as eco-friendly, remains problematic, with biodegradation often requiring industrial conditions. Enzyme-based depolymerization of polyesters, such as polyethylene terephthalate (PET) and bioplastics (e.g., polylactic acid (PLA), poly(butylene adipate-co-terephthalate) (PBAT), and polyhydroxyalkanoates (PHAs)), has emerged as a promising alternative, offering a green approach to postconsumer plastic management with a reduced environmental impact and in alignment with circular economy principles. This review summarizes recent advances in enzymatic degradation of oil-derived and bio-based polyesters. Key recent developments are discussed including novel high-throughput screenings, computational workflow for improvement of PET hydrolases and de novo design of biocatalysts, microbial platforms, and enzyme-embedded self-biodegrading bioplastics. Collectively, these innovations are redefining the role of biocatalysis in tackling synthetic polymer pollution. Looking ahead, the integration of enzymatic depolymerization with upcycling pathways, standardized kinetic metrics, and one-pot bioprocesses represents a viable strategy for sustainable plastic waste valorization.</p>","PeriodicalId":12187,"journal":{"name":"FEBS Open Bio","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145029392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Short-term actions of epigalocatechin-3-gallate in the liver: a mechanistic insight into hypoglycemic and potential toxic effects.","authors":"Carla Indianara Bonetti, Bruna Lopes Correia, Francielle Cristina Nakamura Manicardi, Nairana Mithieli de Queiroz Eskuarek Melo, Vanesa de Oliveira Pateis, Jurandir Fernando Comar, Anacharis Babeto de Sá-Nakanishi, Adelar Bracht, Lívia Bracht","doi":"10.1002/2211-5463.70118","DOIUrl":"https://doi.org/10.1002/2211-5463.70118","url":null,"abstract":"<p><p>Epigallocatechin-3-gallate (EGCG), the main catechin in green tea, is associated with antidiabetic and anti-obesity effects, although its acute hepatic actions remain unclear. We investigated short-term effects of EGCG (10-500 μm) using isolated perfused rat livers and complementary assays in mitochondrial, microsomal, and cytosolic fractions. EGCG markedly inhibited gluconeogenesis from lactate (up to 52%), glycerol (33%), and alanine (47%), while it stimulated glycolysis, glycogenolysis, and oleic acid oxidation (+42% total ketone bodies). Oxygen uptake was stimulated under glycogenolytic and fatty acid oxidizing conditions but inhibited under gluconeogenic conditions. Mechanistic analyses revealed EGCG-induced mild mitochondrial uncoupling, inhibition of pyruvate carboxylase and glucose-6-phosphatase (with no effect on fructose-1,6-bisphosphatase) and stimulation of phosphoenolpyruvate carboxykinase. EGCG shifted cytosolic and mitochondrial NADH/NAD⁺ ratios toward oxidation, increased mitochondrial and plasma membrane permeability (LDH leakage from 10 μm), and altered redox-sensitive fluxes, while the total hepatic ATP content remained unchanged. In summary, EGCG's multifaceted actions suggest that suppression of gluconeogenesis may contribute to its antihyperglycemic effect and the stimulation of fatty acid oxidation to its anti-obesity action. Finally, EGCG's membrane-disruptive properties raise concerns about potential hepatotoxicity in compromised livers.</p>","PeriodicalId":12187,"journal":{"name":"FEBS Open Bio","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145014211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stromagenesis and cancer-associated fibroblast heterogeneity in primary tumors and metastasis: focus in non-small cell lung cancer","authors":"Alejandro Bernardo,&nbsp;Natalia Díaz-Valdivia,&nbsp;Patricia Fernández-Nogueira,&nbsp;Jordi Alcaraz","doi":"10.1002/2211-5463.70102","DOIUrl":"10.1002/2211-5463.70102","url":null,"abstract":"<p>Non-small cell lung cancer (NSCLC) is the most common lung cancer type and one of the deadliest neoplasias worldwide. NSCLC is histologically classified into adenocarcinoma, squamous cell carcinoma, and other less frequent subtypes. Both subtypes and other solid tumors are increasingly regarded as abnormal organs, highlighting the critical role of the desmoplastic tumor stroma rich in cancer-associated fibroblasts (CAFs) in driving tumor progression and therapeutic resistance. This tumor stroma resembles a chronic fibrotic wound and is largely formed by activated/myofibroblast-like α-SMA+ CAFs (myCAFs), which are strongly associated with immunosuppression and poor prognosis. Despite the dominance of the myCAF phenotype, we reported a decade ago phenotypic alterations in NSCLC with a strong dependence on the histologic subtype. Subsequent studies using functional assays, single-cell techniques, and <i>in vivo</i> models have refined these initial observations, enhancing our understanding of the biology of both normal fibroblasts/myofibroblasts and CAFs in NSCLC and other cancer types, including their origins, subclassification, and physiopathologic functions. Notably, increasing evidence supports that CAFs can exhibit tumor-restraining or tumor-promoting effects, and current therapeutic efforts aim to shift the balance towards tumor-restraining phenotypes. Here, we review major advances in our understanding of tumor stromagenesis and CAF heterogeneity in both primary tumors and metastasis, including emerging consensus, with a special focus on NSCLC and its frequent dissemination to the brain. We also highlight the critical role of smoking through epigenetic reprogramming of the TGF-β/SMAD3 pathway. These advances are beginning to delineate how CAF heterogeneity depends on the stage and histologic subtype in NSCLC.</p>","PeriodicalId":12187,"journal":{"name":"FEBS Open Bio","volume":"15 10","pages":"1599-1617"},"PeriodicalIF":2.3,"publicationDate":"2025-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://febs.onlinelibrary.wiley.com/doi/epdf/10.1002/2211-5463.70102","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145000005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Non-coding RNAs as key player in cancer diagnosis and treatment","authors":"Marcin Majka","doi":"10.1002/2211-5463.70088","DOIUrl":"10.1002/2211-5463.70088","url":null,"abstract":"<p>Most of the human genome is transcribed into noncoding RNAs (ncRNAs), which although do not encode proteins, are often involved in key cellular processes. Recent technological advances have accelerated the discovery of ncRNA as well as our understanding of ncRNAs as cancer biomarkers and targets. In this “In the Limelight” special issue of <i>FEBS Open Bio</i>, four review articles by leading experts provide an overview of the role of ncRNAs in cancer, from their association to liver cancer to their role as oncogenes in head and neck squamous cell carcinoma (HNSCC).</p>","PeriodicalId":12187,"journal":{"name":"FEBS Open Bio","volume":"15 9","pages":"1380-1382"},"PeriodicalIF":2.3,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://febs.onlinelibrary.wiley.com/doi/epdf/10.1002/2211-5463.70088","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144927635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diffusion-based size determination of solute particles: a method adapted for postsynaptic proteins.","authors":"András László Szabó, Eszter Nagy-Kanta, Soma Varga, Edit Andrea Jáger, Csaba István Pongor, Mária Laki, András József Laki, Zoltán Gáspári","doi":"10.1002/2211-5463.70111","DOIUrl":"https://doi.org/10.1002/2211-5463.70111","url":null,"abstract":"<p><p>The postsynaptic density (PSD) is a complex, multilayered protein network largely situated on the internal surface of the postsynaptic membrane. It is the first processing unit for incoming synaptic signals, and changes in its internal structure are associated with synaptic strength and plasticity. These structural changes are largely governed by multivalent interactions between its components. The in vitro characterization of such complexes requires unbiased methods that can be used to estimate the size of the emerging assemblies for systems with multiple possible stoichiometries. Here, we present an experimental method for detecting specific PSD proteins as well as their complexes based on their diffusion in a microfluidic environment. The method requires a fluorescent labeling technique that does not disrupt the function of labeled proteins, a microfluidic device that can maintain laminar flow for protein solutions, a microscope that can record the fluorescent signal emitted by these solutions, and an analytic software package that can process the collected experimental data and convert them into approximate particle sizes. We demonstrate the applicability of our method on protein constructs of various postsynaptic proteins, including the multivalent assembly between GKAP and LC8.</p>","PeriodicalId":12187,"journal":{"name":"FEBS Open Bio","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144948094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Key role for inhibins in effective T cell activation, migration and Th17 differentiation.","authors":"Sandra Ortega-Francisco, Roxana Olguín-Alor, Lizbeth Bolaños-Castro, Alexandra Morales-Cruz, Marisol De La Fuente-Granada, Gloria Soldevila","doi":"10.1002/2211-5463.70106","DOIUrl":"https://doi.org/10.1002/2211-5463.70106","url":null,"abstract":"<p><p>Our group has previously reported that inhibin and its molecular pair, TGF-β type III receptor (TβRIII), regulate T cell development within the thymus. In addition, inhibins play a key role in immune tolerance through the modulation of dendritic cell (DC) maturation and peripheral Treg induction. However, the functional role of inhibins in T cell activation and differentiation is currently unknown. Here, we demonstrate that inhibins are produced by activated T cells and play a role during T cell activation, migration, and functional Th differentiation. Specifically, stimulation of Inhα^-/- naïve T cells resulted in decreased expression of early activation markers, including CD69, CD25, and TβRIII, compared to Inhα^+/+ T cells. Additionally, we analyzed the migratory potential of Inhα^-/- T cells toward CCR7 ligands and showed an impaired in vitro chemotaxis toward CCL21 and CCL19, which correlated with a decreased homing to peripheral lymph nodes using in vivo competitive assays. To evaluate the impact of inhibins on Th differentiation, we performed in vitro polarization cultures under skewing conditions. Interestingly, Inhα^-/- naïve T cells showed a decreased differentiation to Th1 cells, while the induction of Th17 cells was significantly increased when compared with Inhα^+/+. This preferential polarization of Inhα^-/- toward Th17 was reversed by the addition of recombinant Inh A, without altering the differentiation of Inhα^-/- Th1. Our data demonstrate that inhibins regulate T cell effector differentiation and homing and thus, may be considered new players in T cell immune responses.</p>","PeriodicalId":12187,"journal":{"name":"FEBS Open Bio","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144948084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of the thermophilic xylanase Fsa02490Xyn from the hyperthermophile Fervidibacter sacchari belonging to glycoside hydrolase family 10","authors":"Nicole Torosian,&nbsp;Jonathan K. Covington,&nbsp;Allison M. Cook,&nbsp;Nancy O. Nou,&nbsp;Marike Palmer,&nbsp;Ritesh Mewalal,&nbsp;Miranda Harmon-Smith,&nbsp;Ian K. Blaby,&nbsp;Jan-Fang Cheng,&nbsp;Matthias Hess,&nbsp;Brian P. Hedlund","doi":"10.1002/2211-5463.70072","DOIUrl":"10.1002/2211-5463.70072","url":null,"abstract":"<div>\u0000 \u0000 <section>\u0000 \u0000 <p><i>Fervidibacter sacchari</i> is an aerobic hyperthermophile belonging to the phylum <i>Armatimonadota</i> that degrades a variety of polysaccharides. Its genome encodes 117 enzymes with one or more annotated glycoside hydrolase (GH) domain, but the roles of these putative GHs in polysaccharide catabolism are poorly defined. Here, we describe one <i>F. sacchari</i> enzyme encoding a GH10 domain, Fsa02490Xyn, that was previously shown to be active on <i>Miscanthus</i>, oat β-glucan, and beech-wood xylan, with optimal activity at 90–100 °C. We show that Fsa02490Xyn is also active on birch-wood xylan and gellan gum. The pH range on beech-wood xylan was 4.5 to 9.5 (pH_opt 7.0–8.0). Fsa024940Xyn had a <i>K</i>_m of 2.375 m<span>m</span>, <i>V</i>_max of 1250 μ<span>m</span>·min⁻¹, and <i>k</i>_cat/<i>K</i>_m of 1.259 × 10⁴ s⁻¹·<span>m</span>⁻¹ when using a <i>para</i>-nitrophenyl-𝛽-xylobioside assay. A phylogenetic analysis of GH10 family enzymes revealed a large clade of enzymes from diverse members of the class <i>Fervidibacteria</i>, including Fsa02490Xyn and a second enzyme from <i>F. sacchari</i>, with apparent horizontal gene transfer within <i>Fervidibacteria</i> and between <i>Fervidibacteria</i> and thermophilic <i>Bacillota</i>. This study establishes Fsa02490Xyn as a hyperthermophilic GH10 enzyme with endo-β-1,4-xylanase activity and identifies a large clade of homologous GH10 enzymes within the class <i>Fervidibacteria</i>.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <div>\u0000 <div>\u0000 \u0000 <h3>Impact statement</h3>\u0000 <p>The depolymerization of xylan at high temperatures is important because this process limits the degradation of polysaccharides in nature and the synthesis of biofuels from plant wastes. Our study is also important because <i>F. sacchari</i> is one of only a few cultivated members of the <i>Armatimonadota</i>, which are polysaccharide-degradation specialists.</p>\u0000 </div>\u0000 </div>\u0000 </section>\u0000 </div>","PeriodicalId":12187,"journal":{"name":"FEBS Open Bio","volume":"15 10","pages":"1629-1642"},"PeriodicalIF":2.3,"publicationDate":"2025-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://febs.onlinelibrary.wiley.com/doi/epdf/10.1002/2211-5463.70072","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144948102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Osmotic pressure-induced calcium response states","authors":"Zidan Gao,&nbsp;Keiji Naruse,&nbsp;Masatoshi Morimatsu","doi":"10.1002/2211-5463.70094","DOIUrl":"10.1002/2211-5463.70094","url":null,"abstract":"<p>Osmotic pressure is essential for maintaining cellular homeostasis; however, the mechanisms by which cells sense and respond to acute osmotic stress remain incompletely understood. Here, we applied rapid osmotic pressure stimulation to cultured HEK293T cells and observed dynamic intracellular calcium responses. Acute hypotonic stimulation evoked calcium response patterns, whereas hypertonic and isotonic stress did not elicit similar effects. Mechanistically, these calcium signals originated from the endoplasmic reticulum via ryanodine receptor 2 and propagated to neighboring cells through Connexin 43-mediated gap junctions. These findings reveal a previously unrecognized role for calcium signaling in the acute cellular response to osmotic stress, providing new insights into the mechanisms of intercellular communication during osmotic adaptation.</p>","PeriodicalId":12187,"journal":{"name":"FEBS Open Bio","volume":"15 10","pages":"1714-1722"},"PeriodicalIF":2.3,"publicationDate":"2025-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://febs.onlinelibrary.wiley.com/doi/epdf/10.1002/2211-5463.70094","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144948217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}