FEBS Open Bio最新文献_第10页

Expression and purification of E140 protein antigen fragments of Plasmodium vivax and Plasmodium berghei for serological assays 间日疟原虫和伯氏疟原虫E140蛋白抗原片段的表达和纯化及血清学检测。

IF 2.8 4区生物学

FEBS Open Bio Pub Date : 2025-01-15 DOI: 10.1002/2211-5463.13939

Rodolfo Ferreira Marques, Edit Ábrahám, Hiromi Muramatsu, Daniel Youssef Bargieri, Norbert Pardi, Zoltán Lipinszki

{"title":"Expression and purification of E140 protein antigen fragments of Plasmodium vivax and Plasmodium berghei for serological assays","authors":"Rodolfo Ferreira Marques, Edit Ábrahám, Hiromi Muramatsu, Daniel Youssef Bargieri, Norbert Pardi, Zoltán Lipinszki","doi":"10.1002/2211-5463.13939","DOIUrl":"10.1002/2211-5463.13939","url":null,"abstract":"Malaria, a life-threatening disease caused by Plasmodium parasites, continues to pose a significant global health threat, with nearly 250 million infections and over 600 000 deaths reported annually by the WHO. Fighting malaria is particularly challenging partly due to the complex life cycle of the parasite. However, technological breakthroughs such as the development of the nucleoside-modified mRNA lipid nanoparticle (mRNA-LNP) vaccine platform, along with the discovery of novel conserved Plasmodium antigens such as the E140 protein, present new opportunities in malaria prevention. Importantly, production of recombinant proteins for malaria vaccine evaluation by serological assays often represents an additional hurdle because many Plasmodium proteins are complex and often contain transmembrane domains that make production and purification particularly difficult. This research protocol provides a step-by-step guide for the production and purification of P. berghei and P. vivax E140 protein fragments that can be used to test humoral immune responses against this novel malaria vaccine target. We demonstrate that the purified proteins can be successfully used in enzyme-linked immunosorbent assay (ELISA) to evaluate antigen-specific binding antibody responses in sera obtained from E140 mRNA-LNP-vaccinated mice. Therefore, these proteins can contribute to the development and evaluation of E140-based malaria vaccines.","PeriodicalId":12187,"journal":{"name":"FEBS Open Bio","volume":"15 5","pages":"690-698"},"PeriodicalIF":2.8,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/2211-5463.13939","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143002709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of synaptotagmin-1 action models by all-atom molecular dynamics simulations 全原子分子动力学模拟评价synaptotagmin-1作用模型。

IF 2.8 4区生物学

FEBS Open Bio Pub Date : 2025-01-15 DOI: 10.1002/2211-5463.13966

Josep Rizo, Klaudia Jaczynska, Christian Rosenmund

{"title":"Evaluation of synaptotagmin-1 action models by all-atom molecular dynamics simulations","authors":"Josep Rizo, Klaudia Jaczynska, Christian Rosenmund","doi":"10.1002/2211-5463.13966","DOIUrl":"10.1002/2211-5463.13966","url":null,"abstract":"Neurotransmitter release is triggered in microseconds by the two C2 domains of the Ca2+ sensor synaptotagmin-1 and by SNARE complexes, which form four-helix bundles that bridge the vesicle and plasma membranes. The synaptotagmin-1 C2B domain binds to the SNARE complex via a ‘primary interface’, but the mechanism that couples Ca2+-sensing to membrane fusion is unknown. Widespread models postulate that the synaptotagmin-1 Ca2+-binding loops accelerate membrane fusion by inducing membrane curvature, perturbing lipid bilayers or helping bridge the membranes, but these models do not seem compatible with SNARE binding through the primary interface, which orients the Ca2+-binding loops away from the fusion site. To test these models, we performed molecular dynamics simulations of SNARE complexes bridging a vesicle and a flat bilayer, including the synaptotagmin-1 C2 domains in various configurations. Our data do not support the notion that insertion of the synaptotagmin-1 Ca2+-binding loops causes substantial membrane curvature or major perturbations of the lipid bilayers that could facilitate membrane fusion. We observed membrane bridging by the synaptotagmin-1 C2 domains, but such bridging or the presence of the C2 domains near the site of fusion hindered the action of the SNAREs in bringing the membranes together. These results argue against models predicting that synaptotagmin-1 triggers neurotransmitter release by inducing membrane curvature, perturbing bilayers or bridging membranes. Instead, our data support the hypothesis that binding via the primary interface keeps the synaptotagmin-1 C2 domains away from the site of fusion, orienting them such that they trigger release through a remote action.","PeriodicalId":12187,"journal":{"name":"FEBS Open Bio","volume":"15 5","pages":"699-713"},"PeriodicalIF":2.8,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/2211-5463.13966","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143002703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

FSH enhances the inflammatory response of macrophages in the knee joint possibly through the NFκB pathway FSH可能通过nf - κ b途径增强膝关节巨噬细胞的炎症反应。

IF 2.8 4区生物学

FEBS Open Bio Pub Date : 2025-01-13 DOI: 10.1002/2211-5463.13959

Yu Chen, Na Xu, Wen-wen Zhang, Yan Wang, Tong Su, Yan-man Zhou, Jin Xu

{"title":"FSH enhances the inflammatory response of macrophages in the knee joint possibly through the NFκB pathway","authors":"Yu Chen, Na Xu, Wen-wen Zhang, Yan Wang, Tong Su, Yan-man Zhou, Jin Xu","doi":"10.1002/2211-5463.13959","DOIUrl":"10.1002/2211-5463.13959","url":null,"abstract":"Previous studies have suggested that women with higher follicle-stimulating hormone (FSH) levels have a greater incidence of osteoarthritis (OA) compared to women with lower FSH despite normal estrogen levels. Our previous studies also showed that FSH has a negative effect on cartilage in postmenopausal OA. However, no studies have investigated the effect of FSH on the synovium. Here, we showed that the FSH receptor (FSHR) is expressed on RAW264.7 cells and BMDM (Bone Marrow-Derived Macrophages), and found that FSH stimulation promotes the production and secretion of inflammatory cytokines in synovial macrophages. In RAW264.7 cells, FSH stimulation enhances phosphorylation and nuclear translocation of P65, suggesting the activation of NFκB signaling, while the knockdown of FSHR eliminates the proinflammatory effect of FSH. To further validate these results, we used an ovariectomy mouse model supplemented with FSH and estrogen, and a mouse model with FSH neutralization. We noted that FSHR was expressed on mouse synovial joint membranes. Furthermore, in ovariectomy mice supplemented with estrogen and treated with FSH, synovial macrophages were significantly increased, while the opposite was the case in the FSH neutralizing group, which suggest that FSH triggers an inflammatory response in the synovial tissue in mice. Taken together, our results indicate that FSH is an important regulator in synovial inflammation via NFκB signaling activation and, to some extent, appears to accelerate the development of osteoarthritis.","PeriodicalId":12187,"journal":{"name":"FEBS Open Bio","volume":"15 4","pages":"622-633"},"PeriodicalIF":2.8,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/2211-5463.13959","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142970319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Purification and characterization of a thermophilic NAD+-dependent lactate dehydrogenase from Moorella thermoacetica 热醋酸摩尔氏菌嗜热NAD+乳酸脱氢酶的纯化及特性研究。

IF 2.8 4区生物学

FEBS Open Bio Pub Date : 2025-01-13 DOI: 10.1002/2211-5463.13964

Florian P. Rosenbaum, Volker Müller

{"title":"Purification and characterization of a thermophilic NAD+-dependent lactate dehydrogenase from Moorella thermoacetica","authors":"Florian P. Rosenbaum, Volker Müller","doi":"10.1002/2211-5463.13964","DOIUrl":"10.1002/2211-5463.13964","url":null,"abstract":"Oxidation of lactate under anaerobic dark fermentative conditions poses an energetic problem. The redox potential of the lactate/pyruvate couple is too electropositive to reduce the physiological electron carriers NAD(P)+ or ferredoxin. However, the thermophilic, anaerobic, and acetogenic model organism Moorella thermoacetica can grow on lactate but was suggested to have a NAD+-dependent lactate dehydrogenase (LDH), based on enzyme assays in cell-free extract. LDHs of thermophilic and anaerobic bacteria are barely characterized but have a huge biotechnological potential. Here, we have purified the LDH from M. thermoacetica by classical chromatography. Lactate-dependent NAD+ reduction was observed with high rates. Electron bifurcation was not observed. At pH 8 and 65 °C, the LDH had a specific activity of 60 U·mg−1 for lactate oxidation, but NADH-driven pyruvate reduction was around four times faster with an activity of 237 U·mg−1. Since lactate formation is preferred by the enzyme, further modifications of the LDH can be suggested to improve the kinetics of this enzyme making it a promising candidate for biotechnological applications.","PeriodicalId":12187,"journal":{"name":"FEBS Open Bio","volume":"15 5","pages":"714-725"},"PeriodicalIF":2.8,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/2211-5463.13964","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142970320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Meeting Abstract 焦点：教育。

IF 2.8 4区生物学

FEBS Open Bio Pub Date : 2025-01-06 DOI: 10.1002/2211-5463.13954

引用次数: 0

Strengthening the bond with the scientific community: FEBS Open Bio in 2025 加强与科学界的联系：2025年的FEBS开放生物

IF 2.8 4区生物学

FEBS Open Bio Pub Date : 2025-01-06 DOI: 10.1002/2211-5463.13956

Sara Fuentes, Miguel A. De la Rosa

引用次数: 0

CD44v, S1PR1, HER3, MET and cancer-associated amino acid transporters are promising targets for the pancreatic cancers characterized using mAb CD44v, S1PR1, HER3， MET和癌症相关的氨基酸转运蛋白是用单抗表征胰腺癌的有希望的靶点。

IF 2.8 4区生物学

FEBS Open Bio Pub Date : 2025-01-05 DOI: 10.1002/2211-5463.13963

Takashi Nakano, Kouki Okita, Shogo Okazaki, Soshi Yoshimoto, Sachiko Masuko, Hideki Yagi, Kazunori Kato, Yoshihisa Tomioka, Kenichi Imai, Yoichi Hamada, Kazue Masuko, Kayoko Shimada-Takaura, Noriaki Nagai, Hideyuki Saya, Tomio Arai, Toshiyuki Ishiwata, Takashi Masuko

{"title":"CD44v, S1PR1, HER3, MET and cancer-associated amino acid transporters are promising targets for the pancreatic cancers characterized using mAb","authors":"Takashi Nakano, Kouki Okita, Shogo Okazaki, Soshi Yoshimoto, Sachiko Masuko, Hideki Yagi, Kazunori Kato, Yoshihisa Tomioka, Kenichi Imai, Yoichi Hamada, Kazue Masuko, Kayoko Shimada-Takaura, Noriaki Nagai, Hideyuki Saya, Tomio Arai, Toshiyuki Ishiwata, Takashi Masuko","doi":"10.1002/2211-5463.13963","DOIUrl":"10.1002/2211-5463.13963","url":null,"abstract":"Effective therapies have yet to be established for pancreatic ductal adenocarcinomas (PDAC) even though it is the most aggressive cancer. In the present study, PDAC was analyzed using novel rat mAbs against membrane proteins in conjunction with flow cytometry and immunohistochemistry. Human epidermal growth receptor (HER)1–4, mesenchymal to epithelial transition factor (MET), sphingosine-1-phospahate receptor 1 (S1PR1), l-type amino acid transporter 1 (LAT1), system x−c transporter (xCT), alanine-serine-cysteine transporter (ASCT2), cationic amino acid transporter 1 (CAT1) and variant CD44 (CD44v) were expressed at high frequencies in both in vitro and in vivo PDAC. Internalization of membrane proteins by mAbs and growth inhibition by toxin-linked mAbs were demonstrated in many PDAC cell lines, and mAbs against S1PR1, ASCT2, HER3 and CD44v inhibited the growth of xenografted MIA PaCa-2 PDAC cells. Furthermore, CD44v-high PDAC showed high mRNA expression of HER1–3, MET and CD44v, and was correlated with poor prognosis. Taken together, our results suggest that CD44v, S1PR1, HER3, MET and the above-mentioned cancer-associated amino acid transporters might be promising targets for the diagnosis and treatment of PDAC.","PeriodicalId":12187,"journal":{"name":"FEBS Open Bio","volume":"15 5","pages":"867-884"},"PeriodicalIF":2.8,"publicationDate":"2025-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/2211-5463.13963","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142930863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CDK5 interacts with MST2 and modulates the Hippo signalling pathway CDK5与MST2相互作用并调节Hippo信号通路。

IF 2.8 4区生物学

FEBS Open Bio Pub Date : 2024-12-30 DOI: 10.1002/2211-5463.13962

Mehak Passi, Jan B. Stöckl, Thomas Fröhlich, Simone Moser, Angelika M. Vollmar, Stefan Zahler

{"title":"CDK5 interacts with MST2 and modulates the Hippo signalling pathway","authors":"Mehak Passi, Jan B. Stöckl, Thomas Fröhlich, Simone Moser, Angelika M. Vollmar, Stefan Zahler","doi":"10.1002/2211-5463.13962","DOIUrl":"10.1002/2211-5463.13962","url":null,"abstract":"MST2 (STK3) is a major upstream kinase in the Hippo signalling pathway, an evolutionary conserved pathway in regulation of organ size, self-renewal and tissue homeostasis. Its downstream effectors are the transcriptional regulators YAP and TAZ. This pathway is regulated by a variety of factors, such as substrate stiffness or cell–cell contacts. Using a yeast two-hybrid screen, we detected a novel interaction between the kinases MST2 and CDK5, which we further confirmed by co-immunoprecipitation experiments. Cyclin-dependent kinase 5 (CDK5) is an unusual member of the family of cyclin-dependent kinases, involved in tumour growth and angiogenesis. Although a link between CDK5 and Hippo has been previously postulated, the mode of action is still elusive. Here, we show that knockdown of CDK5 causes reduced transcriptional activity of YAP and that CDK5 influences the phosphorylation levels of the Hippo upstream kinase LATS1. Moreover, a phosphoproteomics approach revealed that CDK5 interferes with the phosphorylation of DLG5, another upstream kinase, which regulates the Hippo pathway. Hence, CDK5 seems to act as a signalling hub for integrating the Hippo pathway and other signalling cascades. These interactions might have important implications for the use of CDK5 inhibitors, which are already in clinical use for tumour diseases.","PeriodicalId":12187,"journal":{"name":"FEBS Open Bio","volume":"15 4","pages":"647-660"},"PeriodicalIF":2.8,"publicationDate":"2024-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/2211-5463.13962","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142909455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancing human ACE2 expression in mouse models to improve COVID-19 research 增强人ACE2在小鼠模型中的表达以改善COVID-19研究。

IF 2.8 4区生物学

FEBS Open Bio Pub Date : 2024-12-29 DOI: 10.1002/2211-5463.13934

Sun Jiaoyang, Cheng Shaofei, Hong Guangliang, Quan Xiongzhi, Lin Haofeng, Mao Rui, Johannes Grillari, Shi Zheng-Li, Chen Jiekai, Liu Meiqin, Wu Haoyu, Wu Guangming

{"title":"Enhancing human ACE2 expression in mouse models to improve COVID-19 research","authors":"Sun Jiaoyang, Cheng Shaofei, Hong Guangliang, Quan Xiongzhi, Lin Haofeng, Mao Rui, Johannes Grillari, Shi Zheng-Li, Chen Jiekai, Liu Meiqin, Wu Haoyu, Wu Guangming","doi":"10.1002/2211-5463.13934","DOIUrl":"10.1002/2211-5463.13934","url":null,"abstract":"Mice are one of the most common biological models for laboratory use. However, wild-type mice are not susceptible to COVID-19 infection due to the low affinity of mouse ACE2, the entry protein for SARS-CoV-2. Although mice with human ACE2 (hACE2) driven by Ace2 promoter reflect its tissue specificity, these animals exhibit low ACE2 expression, potentially limiting their fidelity in mimicking COVID-19 manifestations and their utility in viral studies. Here, we created and compared hACE2 mouse models generated with different strategies. Our findings show that distinct β-globin insertion within hACE2 cassette significantly influences its expression, with downstream placement enhancing transcription. Moreover, optimizing hACE2 codons (opt-hACE2) improves translation efficiency in multiple tissues. Notably, opt-hACE2 mice displayed more active immune responses and severe COVID-19 phenotypes following SARS-CoV-2 challenge compared to other models. Our study demonstrates the dual regulatory role of β-globin element in transgene transcription and suggests that opt-hACE2 mice might serve as valuable tools for SARS-CoV-2 research.","PeriodicalId":12187,"journal":{"name":"FEBS Open Bio","volume":"15 2","pages":"324-334"},"PeriodicalIF":2.8,"publicationDate":"2024-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/2211-5463.13934","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142906842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

RETRACTION: Effect of a Neural Relay on Liver Regeneration in Mice: Activation of Serotonin Release from the Gastrointestinal Tract 神经接力对小鼠肝脏再生的影响：胃肠道血清素释放的激活。

IF 2.8 4区生物学

FEBS Open Bio Pub Date : 2024-12-25 DOI: 10.1002/2211-5463.13961

{"title":"RETRACTION: Effect of a Neural Relay on Liver Regeneration in Mice: Activation of Serotonin Release from the Gastrointestinal Tract","authors":"","doi":"10.1002/2211-5463.13961","DOIUrl":"10.1002/2211-5463.13961","url":null,"abstract":"RETRACTION: R. Inoue, K. Kamimura, T. Nagoya, N. Sakai, T. Yokoo, R. Goto, K. Ogawa, Y. Shinagawa-Kobayashi, Y. Watanabe-Mori, A. Sakamaki, S. Abe, H. Kamimura, N. Miyamura, H. Nishina, and S. Terai, “Effect of a Neural Relay on Liver Regeneration in Mice: Activation of Serotonin Release from the Gastrointestinal Tract,” FEBS Open Bio 8, no. 3 (2018): 449-460, https://doi.org/10.1002/2211-5463.12382.The above article, published online on 16 January 2018, in Wiley Online Library (http://onlinelibrary.wiley.com/),has been retracted by agreement between the journal Editor-in-Chief, Miguel A. De la Rosa; FEBS Press; and John Wiley and Sons Ltd. The journal received a report from a third party which indicated that the Day 2 and Day 4 panels in Figure 3A had been duplicated and rotated. Additional investigation by the journal discovered multiple inappropriate image duplications and overlaps in Figure 6A. The authors responded to an inquiry by the journal, confirmed that images had been duplicated in both figures, and provided what was labelled as the correct data. Following receipt of the authors' explanation and new data, the journal requested an investigation by the authors' institution.The institutional investigation reported that there was insufficient evidence of intentional image manipulation and concluded that the image duplications were due to errors in image preparation by the authors. However, given the extent of the identified issues, the editors have lost confidence in the data presented and consider the conclusions of this manuscript substantially compromised. As a result, the Editor-in-Chief, FEBS Press, and John Wiley and Sons Ltd. have determined that a retraction is necessary. The authors disagree with the retraction.","PeriodicalId":12187,"journal":{"name":"FEBS Open Bio","volume":"15 5","pages":"889"},"PeriodicalIF":2.8,"publicationDate":"2024-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/2211-5463.13961","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142893306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0