FEBS Open Bio最新文献

Ultrafast visual detection of Streptococcus suis via HtpsC-targeted LAMP-CRISPR-Cas12a coupled with lateral flow immunochromatography. htpsc靶向LAMP-CRISPR-Cas12a与侧流免疫层析耦联超快速视觉检测猪链球菌

IF 2.7 4区生物学

FEBS Open Bio Pub Date : 2026-10-16 DOI: 10.1002/2211-5463.70353

Liu-Xin Li, Miao Xin, Xiao-Wen Cui, Shi-Yin Yu, Fei Meng, Min Cao, Xiao-Hui Xiong, Yi-Chen Lu

{"title":"Ultrafast visual detection of Streptococcus suis via HtpsC-targeted LAMP-CRISPR-Cas12a coupled with lateral flow immunochromatography.","authors":"Liu-Xin Li, Miao Xin, Xiao-Wen Cui, Shi-Yin Yu, Fei Meng, Min Cao, Xiao-Hui Xiong, Yi-Chen Lu","doi":"10.1002/2211-5463.70353","DOIUrl":"10.1002/2211-5463.70353","url":null,"abstract":"This study describes a rapid, visual detection platform for Streptococcus suis, a significant zoonotic pathogen, by integrating loop-mediated isothermal amplification (LAMP) with CRISPR-Cas12a and a lateral flow immunochromatographic strip (LFIA) readout. Targeting the conserved HtpsC gene, specific LAMP primers and a PAM-compliant CrRNA were designed. Following isothermal amplification, the Cas12a/CrRNA complex recognizes the HtpsC amplicon, initiating target-specific cleavage and subsequent trans-cleavage of a dual-labeled ssDNA reporter (FAM-BIO), enabling visual detection on a LFIA; a design which reduces false positives. The optimized assay detected HtpsC-positive S. suis strains within 45 min and achieved a visual detection limit of 1.1 × 102 CFU/mL under laboratory conditions, with no cross-reactivity observed against the tested nontarget bacteria. In addition to pure-culture dilutions, the assay was further evaluated using spiked porcine blood samples and achieved similar detection performance, supporting its preliminary applicability in a complex biological matrix. Overall, this study provides an HtpsC-targeted visual detection workflow for screening of S. suis and is expected to be further applied to on-site rapid detection of clinical samples.","PeriodicalId":12187,"journal":{"name":"FEBS Open Bio","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2026-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13673931/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149192365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A nationwide student survey on biochemistry learning resources in Italy. 意大利全国学生生物化学学习资源调查。

IF 2.7 4区生物学

FEBS Open Bio Pub Date : 2026-10-16 DOI: 10.1002/2211-5463.70355

Anna Marabotti, Ivano Eberini, Maria Luisa Genova, Francesca Mensitieri, Francesco Malatesta

{"title":"A nationwide student survey on biochemistry learning resources in Italy.","authors":"Anna Marabotti, Ivano Eberini, Maria Luisa Genova, Francesca Mensitieri, Francesco Malatesta","doi":"10.1002/2211-5463.70355","DOIUrl":"10.1002/2211-5463.70355","url":null,"abstract":"Biochemistry is widely recognized as a crucial discipline in life sciences. However, learning biochemistry poses significant challenges, requiring students to understand complex molecular concepts while integrating knowledge across multiple disciplines. This necessitates instructors to innovate their teaching of biochemistry, considering students' learning behaviors. Here, we present the results of a nationwide survey of university students, conducted by the Education Group of the Italian Society of Biochemistry and Molecular Biology, from more than 2000 students across 49 academic institutions in Italy, with the aim of capturing not only which learning resources are relied on but also the underlying motivations for their choices and perceptions of biochemistry. Results show that overall, biochemistry is perceived as an interesting subject to study, even if challenging. Students primarily rely on instructor-provided materials, such as lecture slides, as well as self-acquired resources, including audio recordings and personal notes. Textbooks are used mainly as references when the preferred materials above do not sufficiently support students' learning. AI tools are increasingly used but not yet preferred. This strong reliance on teacher guidance underscores the need for high-quality teaching materials and greater integration of practical, application-oriented learning activities. By mapping these trends, our survey provides a foundation for critical reflection on current teaching practices and offers insights that may guide the development of more effective learning tools. Our broader goal is to support educators in creating environments that foster deeper understanding, sustained engagement, and a more inclusive learning experience in biochemistry.","PeriodicalId":12187,"journal":{"name":"FEBS Open Bio","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2026-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13673930/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149192335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Using fluorescence fluctuation spectroscopy to quantify molecular dynamics within cells. 使用荧光波动光谱来量化细胞内的分子动力学。

IF 2.7 4区生物学

FEBS Open Bio Pub Date : 2026-10-08 DOI: 10.1002/2211-5463.70348

Mark A Hink

引用次数: 0

Fluorination: A strategy to tune the interactions of molecules and nanoscale systems with biological matter. 氟化：一种调整分子和纳米级系统与生物物质相互作用的策略。

IF 2.7 4区生物学

FEBS Open Bio Pub Date : 2026-10-08 DOI: 10.1002/2211-5463.70354

Martina Beccalli, Beatrice Lucia Bona, Francesca Baldelli Bombelli

{"title":"Fluorination: A strategy to tune the interactions of molecules and nanoscale systems with biological matter.","authors":"Martina Beccalli, Beatrice Lucia Bona, Francesca Baldelli Bombelli","doi":"10.1002/2211-5463.70354","DOIUrl":"10.1002/2211-5463.70354","url":null,"abstract":"Fluorination has become a key molecular engineering strategy in medicinal chemistry and nanomedicine, enabling the fine tuning of the physicochemical and biological properties of therapeutic molecules and drug delivery systems. The unique characteristics of fluorine, including its small steric footprint, high electronegativity, and exceptional strength of the C-F bond, enhance metabolic stability, modulate lipophilicity and acidity, and improve pharmacokinetic profiles. Beyond these well-established effects, fluorination has emerged as a tool for controlling the assembly and biological behavior of nanocarriers. Fluorinated biomolecules, polymers, dendrimers, and lipid-based systems often display improved colloidal stability, reduced nonspecific protein adsorption, enhanced cellular uptake, and efficient endosomal escape, ultimately leading to superior intracellular delivery of nucleic acids, proteins, and other therapeutic cargoes. Here we summarize recent advances in the fluorination of both therapeutic cargoes and delivery carriers, emphasizing how different fluorination strategies, including single fluorine atoms, trifluoromethyl groups, linear perfluoroalkyl chains, and emerging short branched fluorinated motifs, can be exploited to optimize drug delivery performance. Attention is devoted to the relationship between fluorinated molecular architectures, self-assembly, and bio-nano interactions that govern biological activity. Furthermore, the advantages and limitations of current fluorination approaches are discussed. Finally, we highlight the need for a deeper mechanistic understanding of how fluorinated moieties interact with proteins and biological membranes. Advanced physicochemical techniques, including neutron and X-ray scattering and reflectometry, are expected to provide fundamental insights into these processes, enabling the rational design of more effective, safer, and environmentally sustainable fluorinated therapeutics and nanomedicines.","PeriodicalId":12187,"journal":{"name":"FEBS Open Bio","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2026-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13647950/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149135735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Selective elimination of quiescent cancer cells by dequalinium chloride via ROS-mediated ferroptosis. 氯去qualinium通过ros介导的铁下垂选择性消除静止癌细胞。

IF 2.7 4区生物学

FEBS Open Bio Pub Date : 2026-10-05 DOI: 10.1002/2211-5463.70344

Kotaro Miyamoto, Shiori Sakai, Minori Endo, Terigele, Mizuho Niibori, Taiki Morita, Yu Goto, Arisa Yoshida, Tomohiro Tanaka, Ning Lin, Takahiro Kuchimaru, Hiroyuki Nakamura, Masahiro Inoue, Shinae Kizaka-Kondoh, Tetsuya Kadonosono

{"title":"Selective elimination of quiescent cancer cells by dequalinium chloride via ROS-mediated ferroptosis.","authors":"Kotaro Miyamoto, Shiori Sakai, Minori Endo, Terigele, Mizuho Niibori, Taiki Morita, Yu Goto, Arisa Yoshida, Tomohiro Tanaka, Ning Lin, Takahiro Kuchimaru, Hiroyuki Nakamura, Masahiro Inoue, Shinae Kizaka-Kondoh, Tetsuya Kadonosono","doi":"10.1002/2211-5463.70344","DOIUrl":"https://doi.org/10.1002/2211-5463.70344","url":null,"abstract":"Quiescent cancer cells (QCCs) drive tumor dormancy and recurrence through their resistance to conventional therapies. Furthermore, because QCCs rely on oxidative phosphorylation and maintain a fragile redox balance, we hypothesized that they harbor a targetable redox vulnerability. Here, we show that the mitochondria-targeted agent dequalinium chloride (DQ) selectively kills QCCs in hypoxia- and nutrient limitation-induced quiescent models, including H2228 cells, multicellular spheroids, and colorectal cancer organoids, while sparing proliferating cells and normal fibroblasts. DQ induces ROS accumulation beyond the survival threshold of QCCs, leading to lipid peroxidation and ferroptotic cell death, whereas proliferating cells undergo limited caspase-independent apoptosis. These findings highlight redox imbalance as an actionable vulnerability of QCCs and position DQ as a potential repurposed agent for eliminating QCCs.","PeriodicalId":12187,"journal":{"name":"FEBS Open Bio","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2026-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13635926/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149105907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In vitro generation of regulatory T cells: A challenging tool for studying immune aging in humans. 体外产生调节性T细胞：研究人类免疫衰老的一个具有挑战性的工具。

IF 2.7 4区生物学

FEBS Open Bio Pub Date : 2026-10-05 DOI: 10.1002/2211-5463.70349

Lynn Muller, Jana Bleher, Birgit Weinberger, Lourdes Rocamora-Reverte

{"title":"In vitro generation of regulatory T cells: A challenging tool for studying immune aging in humans.","authors":"Lynn Muller, Jana Bleher, Birgit Weinberger, Lourdes Rocamora-Reverte","doi":"10.1002/2211-5463.70349","DOIUrl":"https://doi.org/10.1002/2211-5463.70349","url":null,"abstract":"Regulatory T (Treg) cells play a key role in immune tolerance and homeostasis. They prevent exaggerated immune responses, autoimmunity, and are crucial in graft-versus-host responses. On the other hand, their presence in many tumors is associated with a poor prognosis. In aged individuals, Treg populations are modified with a strong bias toward an effector phenotype and a reduced number of naïve Treg cells, but little is known about the functionality of human Treg cells in old age. The low frequency of Treg cells in human peripheral blood is the main limitation to studying them. Therefore, the development of induced Treg (iTreg) cells has become a valuable tool for in vitro research on peripheral Treg cells. Following a gold-standard protocol to generate iTreg in vitro, we aimed to investigate age-related differences in the generation of iTreg cells. However, we detected some unexpected results regarding Foxp3 induction and iTreg functionality. We found no differences in Foxp3+ CD4+ generated cells between young and older individuals. Surprisingly, the presence of the cytokine TGF-β did not play a role in either Foxp3 expression levels or in the suppressive activity of the resulting cells, regardless of the age of the donors. Therefore, we hypothesize that Foxp3 alone does not adequately define bona fide iTreg cells, and the presence of TGF-β is not essential for the in vitro differentiation of human conventional CD4+ T cells into suppressive Foxp3+ cells.","PeriodicalId":12187,"journal":{"name":"FEBS Open Bio","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2026-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13636363/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149105726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Determinants of undergraduate bioscience engagement: Validating attendance barriers and classroom spatial behaviour across institutions. 本科生生物科学参与的决定因素：跨机构验证出勤障碍和教室空间行为。

IF 2.7 4区生物学

FEBS Open Bio Pub Date : 2026-10-03 DOI: 10.1002/2211-5463.70352

Nigel Page, Rachel J Hunt, Lauren J Albee, Daniel Berwick, Alison Snape

{"title":"Determinants of undergraduate bioscience engagement: Validating attendance barriers and classroom spatial behaviour across institutions.","authors":"Nigel Page, Rachel J Hunt, Lauren J Albee, Daniel Berwick, Alison Snape","doi":"10.1002/2211-5463.70352","DOIUrl":"https://doi.org/10.1002/2211-5463.70352","url":null,"abstract":"Student engagement with in-person teaching remains a key challenge in undergraduate bioscience education, particularly in large-cohort and commuter-dominated contexts. This study evaluates a dual-mechanism framework encompassing macro-level structural determinants influencing attendance and micro-level affective determinants influencing in-class spatial behaviour. Survey data were collected from 891 undergraduate bioscience students across three London universities. Attendance barriers were assessed using ranked-choice responses across 15 pedagogic factors and analysed using weighted priority scores. Seating behaviour was captured through self-reported habitual classroom positioning. Non-parametric analyses examined variation across institutional, commuting, demographic, and health-related variables. Consistent with the hypothesis, attendance was primarily associated with macro-level academic and structural factors, with workload management and reliance on recorded teaching emerging as the highest-priority barriers across all institutions. Institutional differences reflected variation in lecture-capture provision and teaching practices. Journey length was associated with academic readiness barriers, particularly for medium-distance commuters, while demographic characteristics showed limited independent influence once structural factors were considered. In contrast, seating behaviour was strongly associated with micro-level affective factors, particularly social anxiety and sensitivity to crowding. Students reporting these factors tended to select peripheral or low-density areas, with minimal influence from institutional or commuting variables. These findings support a dual-dimensional model in which attendance is shaped by structural conditions, while spatial behaviour reflects sensory and emotional factors, with implications for lecture-capture, timetabling and inclusive learning environments in bioscience teaching.","PeriodicalId":12187,"journal":{"name":"FEBS Open Bio","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2026-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13633484/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149105562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Refinement of amino-acid conformation vs. difference density maps in time-resolved serial femtosecond crystallography data analysis 时间分辨序列飞秒晶体学数据分析中氨基酸构象与差密度图的改进。

IF 2.7 4区生物学

FEBS Open Bio Pub Date : 2026-10-01 Epub Date: 2026-06-05 DOI: 10.1002/2211-5463.70250

Meng Iao Fong, Yuhei Hosokawa, Lars Oliver Essen, Manuel Maestre-Reyna

引用次数: 0

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data 分析系列晶体学数据中小构象变化和低占位态的意义。

IF 2.7 4区生物学

FEBS Open Bio Pub Date : 2026-10-01 Epub Date: 2026-03-11 DOI: 10.1002/2211-5463.70218

Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke

引用次数: 0

Lipopolysaccharide uptake is augmented in lipopolysaccharide-tolerant mouse macrophage-like cells via increased CD14 expression 脂多糖耐受小鼠巨噬细胞样细胞通过增加CD14表达增加脂多糖摄取。