FEBS Open Bio最新文献

Production of antibacterial compounds by a Steely hybrid polyketide synthase in Dictyostelium. Dictyostelium中钢杂化聚酮合成酶的抗菌化合物生产。

IF 2.3 4区生物学

FEBS Open Bio Pub Date : 2025-10-08 DOI: 10.1002/2211-5463.70124

Tomoaki R Yamashita, Toyonobu Usuki, Robert R Kay, Tamao Saito

{"title":"Production of antibacterial compounds by a Steely hybrid polyketide synthase in Dictyostelium.","authors":"Tomoaki R Yamashita, Toyonobu Usuki, Robert R Kay, Tamao Saito","doi":"10.1002/2211-5463.70124","DOIUrl":"https://doi.org/10.1002/2211-5463.70124","url":null,"abstract":"Ecological interactions in the soil are often mediated by small molecules, which can later become valuable drugs. The cellular slime mould Dictyostelium discoideum is a soil microbe with a life cycle consisting of unicellular (amoeba) and multicellular phases (fruiting bodies). After Dictyostelium amoebae have consumed all available bacteria, they form stalked fruiting bodies to aid dispersal of the spores. The dying stalk cells repurpose a hybrid polyketide synthase to make abundant chlorinated metabolites, which persist in their fruiting bodies. The most abundant of the chlorinated metabolites, CDF-1, is a chlorinated dibenzofuran, which was shown to be an effective antimicrobial, being roughly as potent as ampicillin. Here, we identify CDF-2 and -3 by purification, followed by MS and NMR, after increasing their yields by using producer species and growth condition optimisation. Similar to CDF-1, CDF-2 and -3 are chlorinated dibenzofurans and exhibit more potent antibacterial activity against Gram-positive bacteria than ampicillin. We propose that the ecological function of CDF-2 and -3 is to protect the dormant spores from degradative bacteria.","PeriodicalId":12187,"journal":{"name":"FEBS Open Bio","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145244107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular determinants of signal transduction in tropomyosin receptor kinases. 原肌球蛋白受体激酶信号转导的分子决定因素。

IF 2.3 4区生物学

FEBS Open Bio Pub Date : 2025-10-06 DOI: 10.1002/2211-5463.70135

Giray Enkavi

引用次数: 0

Overview of molecular signatures of senescence and associated resources: pros and cons. 衰老的分子特征和相关资源综述：利弊。

IF 2.3 4区生物学

FEBS Open Bio Pub Date : 2025-10-04 DOI: 10.1002/2211-5463.70134

Orestis A Ntintas, Sylvia Vagena, Pavlos Pantelis, Giorgos Theocharous, Russel Petty, Konstantinos Evangelou, Vassilis G Gorgoulis

引用次数: 0

Thrombolytic proteins profiling: High-throughput activity, selectivity, and resistance assays. 溶栓蛋白分析：高通量活性，选择性和抗性分析。

IF 2.3 4区生物学

FEBS Open Bio Pub Date : 2025-10-04 DOI: 10.1002/2211-5463.70132

Martin Toul, Alan Strunga, Jiri Damborsky, Zbynek Prokop

{"title":"Thrombolytic proteins profiling: High-throughput activity, selectivity, and resistance assays.","authors":"Martin Toul, Alan Strunga, Jiri Damborsky, Zbynek Prokop","doi":"10.1002/2211-5463.70132","DOIUrl":"https://doi.org/10.1002/2211-5463.70132","url":null,"abstract":"Cardiovascular diseases, including thrombotic events such as ischemic stroke, pulmonary embolism, and myocardial infarction, are among the leading causes of morbidity and disability worldwide. The application of clot-dissolving thrombolytic enzymes is a cost-effective therapeutic intervention for these life-threatening conditions. However, the effectiveness and safety profiles of current drugs are suboptimal, necessitating the discovery of new medicines or the engineering and enhancement of the existing ones. Here, we present a set of optimized biochemical protocols that allow robust screening and the therapeutic potential assessment of thrombolytic biomolecules. The assays provide information on multiple characteristics such as enzymatic activity, fibrinolysis rate, fibrin and fibrinogen stimulation, fibrin selectivity, clot binding affinity, and inhibition resistance. Such detailed characterization enables a rapid and reliable evaluation of candidate effectiveness and provides an indication of biological half-life, associated bleeding complications, and other side effects. We demonstrate the credibility of the methodology by applying it to the two most widely used thrombolytic drugs: alteplase (Activase®/Actilyse®) and tenecteplase (Metalyse®/TNKase®). Consistent with previous studies, tenecteplase exhibited increased fibrin selectivity and inhibition resistance, which explains its extended half-life. Our findings reinforce the growing consensus that tenecteplase may be a superior alternative to alteplase for thrombolytic treatment.","PeriodicalId":12187,"journal":{"name":"FEBS Open Bio","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145225316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Decoding tumor–stroma interactions: from molecular crosstalk to therapeutic targets 解码肿瘤-基质相互作用：从分子串扰到治疗靶点

IF 2.3 4区生物学

FEBS Open Bio Pub Date : 2025-10-01 DOI: 10.1002/2211-5463.70108

Isabel Fabregat

引用次数: 0

Exam-level analysis of lecture capture viewing and student exam performance. 考试水平的分析讲座捕捉观看和学生考试成绩。

IF 2.3 4区生物学

FEBS Open Bio Pub Date : 2025-09-30 DOI: 10.1002/2211-5463.70133

Kirk Hillsley

{"title":"Exam-level analysis of lecture capture viewing and student exam performance.","authors":"Kirk Hillsley","doi":"10.1002/2211-5463.70133","DOIUrl":"https://doi.org/10.1002/2211-5463.70133","url":null,"abstract":"Lecture capture (LC) systems offer students flexible review of lecture content, but their impact on learning outcomes remains mixed. LC engagement and exam performance were analyzed in three in-person courses with LC videos posted for review, each with three lecture blocks and three independent noncumulative exams. Zoom analytics and exam grade data were collected for 299 students across 982 noncumulative exam observations. Four LC metrics were derived per exam: total view duration, number of lectures viewed, number of unique views, and days between access and exam. Average exam scores were compared between LC viewers (n = 216) and nonviewers (n = 83): LC viewers scored significantly higher than nonviewers (66.1% vs. 59.4%). A linear mixed-effects model with student-level random intercepts showed opposing effects of total viewing time (+1.74% per hour) and number of lectures viewed (-1.92% per lecture), implying that average LC view duration per lecture (total minutes watched ÷ lectures viewed) was the strongest predictor of exam score. A post hoc median split of average LC view duration per lecture indicated an 8.02% higher score for students above the median. Decomposition of total LC view time revealed a between-student effect on exam grade (+2.52% per hour) and a within-student effect (-0.84% per hour), showing that spikes above a student's own average view time are associated with a lower exam grade. These findings align with self-regulated learning theory, demonstrating that while greater LC viewing time generally benefits performance, its impact depends on strategic, habitual engagement rather than episodic cramming.","PeriodicalId":12187,"journal":{"name":"FEBS Open Bio","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145198942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Overexpression of CDT1 inhibits cell cycle progression at S phase by interacting with the mini-chromosome maintenance complex and causes DNA damage. CDT1过表达通过与迷你染色体维持复合体相互作用抑制细胞周期在S期的进展并导致DNA损伤。

IF 2.3 4区生物学

FEBS Open Bio Pub Date : 2025-09-26 DOI: 10.1002/2211-5463.70127

Takashi Tsuyama, Nonoka Takayama, Rina Tanaka, Yuuki Arai, Yohko Yamaguchi, Yuko Nawata, Yutaro Azuma, Shusuke Tada

{"title":"Overexpression of CDT1 inhibits cell cycle progression at S phase by interacting with the mini-chromosome maintenance complex and causes DNA damage.","authors":"Takashi Tsuyama, Nonoka Takayama, Rina Tanaka, Yuuki Arai, Yohko Yamaguchi, Yuko Nawata, Yutaro Azuma, Shusuke Tada","doi":"10.1002/2211-5463.70127","DOIUrl":"https://doi.org/10.1002/2211-5463.70127","url":null,"abstract":"Cdc10-dependent transcript 1 (CDT1) is an essential protein for DNA replication licensing, which loads the mini-chromosome maintenance (MCM) complex onto replication origins. We previously reported that excess CDT1 inhibits the elongation of nascent strands during DNA replication in Xenopus egg extracts. In the present study, we investigated the underlying mechanism through which CDT1 inhibits replication fork progression by expressing various CDT1 mutants in human cells. Initiation of DNA replication resulted in downregulation of CDT1, preventing MCM reloading within the same cell cycle; thus, CDT1 overexpression induces rereplication. In this study, we observed that overexpression of a mutant CDT1 lacking licensing activity induced cell cycle arrest at the S phase in human cells. An additional mutation in the MCM-binding domain reduced this cell cycle inhibitory effect. Furthermore, overexpression of CDT1 induced DNA damage independent of its licensing activity. These results suggest that CDT1 overexpression inhibits the progression of replication forks by interacting with the MCM complex, leading to the stalling and collapse of replication forks.","PeriodicalId":12187,"journal":{"name":"FEBS Open Bio","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145174425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

HSP70 governs permeability and mechanotransduction in primary human endothelial cells. HSP70控制原代人内皮细胞的通透性和机械转导。

IF 2.3 4区生物学

FEBS Open Bio Pub Date : 2025-09-26 DOI: 10.1002/2211-5463.70129

Andrea Pinto-Martinez, Everton G Melo, Isadora C B Pavan, Percíllia V S Oliveira, Luiza B C T Coimbra, Thaís L S Araujo

{"title":"HSP70 governs permeability and mechanotransduction in primary human endothelial cells.","authors":"Andrea Pinto-Martinez, Everton G Melo, Isadora C B Pavan, Percíllia V S Oliveira, Luiza B C T Coimbra, Thaís L S Araujo","doi":"10.1002/2211-5463.70129","DOIUrl":"https://doi.org/10.1002/2211-5463.70129","url":null,"abstract":"Vascular barrier disruption is a hallmark of diseases such as cardiovascular disease, stroke, hypertension, pulmonary disorders, infections, and cancer. Endothelium permeability is tightly regulated by shear stress, allowing tissue perfusion, while disturbed flow leads to increased permeability. Cell-cell junctional proteins, including platelet/endothelial cell adhesion molecule-1 (PECAM-1)/CD31 and VE-cadherin, play significant roles in mechanotransduction and barrier integrity. The 70 kDa heat shock protein HSP70 has a well-established cytoprotective function in cardiovascular physiology. Here, we hypothesized that HSP70 interacts with and regulates these junctional proteins. We found that PECAM-1 and VE-cadherin co-immunoprecipitate with endogenous HSP70, and both proteins exhibited positive proximity ligation assay signals in the endothelial monolayers. HSP70 loss of function leads to disassembly of VE-cadherin and PECAM-1 at the cell surface and selectively decreases PECAM-1 steady-state expression. Consistent with its vascular protective role, HSP70 inhibition also reduced endothelial nitric oxide synthase (eNOS) levels. Furthermore, HSP70 was essential for maintaining normal paracellular flux in primary vein (HUVEC) and coronary artery endothelial cells (HCAEC) monolayers, as well as for promoting natural cell alignment under physiological laminar shear stress in HUVEC. These results demonstrate that HSP70 regulates the quality control of interendothelial adherens junctions, mediates responses to hemodynamic forces, and maintains monolayer barrier function across vascular beds. Our findings advance the mechanistic understanding of how human HSP70 mediates vascular homeostasis through endothelium responses to blood flow and permeability in addition to HSP70 role in migration, proliferation, and angiogenesis.","PeriodicalId":12187,"journal":{"name":"FEBS Open Bio","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145148381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cloning, expression, purification, and structural modeling of the Chandipura virus matrix protein. 钱迪普勒病毒基质蛋白的克隆、表达、纯化和结构建模。

IF 2.3 4区生物学

FEBS Open Bio Pub Date : 2025-09-25 DOI: 10.1002/2211-5463.70130

Mariana Grieben

引用次数: 0

Homologous expression and purification of human HAX-1 for structural studies. 人HAX-1的同源表达和纯化用于结构研究。

IF 2.3 4区生物学

FEBS Open Bio Pub Date : 2025-09-25 DOI: 10.1002/2211-5463.70131

Mariana Grieben

引用次数: 0