FEBS Open Bio最新文献_第2页

Positive feedback loop between MAPK and aquaporin 7 regulates autophagy and apoptosis induced by palmitate in RIN-m5f cells. MAPK和水通道蛋白7之间的正反馈回路调节棕榈酸盐诱导的RIN-m5f细胞的自噬和凋亡。

IF 2.8 4区生物学

FEBS Open Bio Pub Date : 2025-03-24 DOI: 10.1002/2211-5463.70011

Maoqi Wang, Jiang Tan, Xueting He, Yuqin Chen, Guoping Qiu, Mei Yang

{"title":"Positive feedback loop between MAPK and aquaporin 7 regulates autophagy and apoptosis induced by palmitate in RIN-m5f cells.","authors":"Maoqi Wang, Jiang Tan, Xueting He, Yuqin Chen, Guoping Qiu, Mei Yang","doi":"10.1002/2211-5463.70011","DOIUrl":"https://doi.org/10.1002/2211-5463.70011","url":null,"abstract":"Type 2 diabetes mellitus (T2DM) is characterized by peripheral blood insulin resistance and progressive pancreatic β-cell dysfunction which is closely related to apoptosis of β-cells. Aquaporin 7 (AQP7) is the only aquaglyceroporin protein expressed in pancreatic β-cells. However, the relationship between AQP7 and autophagy remains unexplored, with limited studies investigating its link to islet β-cell apoptosis. In our study, we utilized an in vitro model involving palmitate-treated rat pancreatic β-cells (RIN-m5f) to examine these relationships. Our aim was to investigate the effects of AQP7 on autophagy and apoptosis by examining LC3 lipidation levels and p62 expression in pancreatic islet β-cells, thereby elucidating potential underlying mechanisms. Our results showed that phosphorylation of p38 and c-Jun-terminal kinase (JNK) increased in response to palmitate treatment, indicating the activation of these signaling pathways. Conversely, AQP7 expression decreased, reduced autophagy, and promoted apoptosis. AQP7 knockdown activated the p38 and JNK signaling pathways, inhibited autophagy (as evidenced by LC3 lipidation and p62 expression), and increased apoptosis. Furthermore, AQP7 overexpression repressed palmitate-induced apoptosis and alleviated autophagy inhibition by suppressing the p38 and JNK mitogen-activated protein kinase (MAPK) signaling pathways. Our results suggest a positive feedback loop between MAPK signaling and AQP7 that regulates autophagy and apoptosis in RIN-m5f cells under high-fat conditions.","PeriodicalId":12187,"journal":{"name":"FEBS Open Bio","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143700110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Possible role of human ribonuclease dicer in the regulation of R loops. 人核糖核酸酶dicer在R环调控中的可能作用。

IF 2.8 4区生物学

FEBS Open Bio Pub Date : 2025-03-24 DOI: 10.1002/2211-5463.70026

Klaudia Wojcik, Paulina Krzeminska, Anna Kurzynska-Kokorniak

{"title":"Possible role of human ribonuclease dicer in the regulation of R loops.","authors":"Klaudia Wojcik, Paulina Krzeminska, Anna Kurzynska-Kokorniak","doi":"10.1002/2211-5463.70026","DOIUrl":"https://doi.org/10.1002/2211-5463.70026","url":null,"abstract":"R loops are three-stranded nucleic acid structures that form naturally in cells under various conditions, mainly as intermediates during replication or as by-products during transcription. R loops are involved in the regulation of many important cellular processes, including replication, transcription, centromere stabilization, protection of chromosome ends, or control of telomere length. Unscheduled R loops are linked to many diseases, including cancer, neurodegenerative, or inflammatory disorders. The list of cancer diseases linked to excessive R loop accumulation is growing rapidly. There is currently much debate about the understanding of abnormal R loop formation and its impact on genome instability and cancer development. In this review, we briefly describe the nature of R loops, their formation under physiological and pathological conditions, and the proteins involved in the regulation of R loops. In addition, we emphasize the possible role of the human ribonuclease Dicer, a multi-tasking protein mostly known for its important role in microRNA biogenesis, in the regulation of R loops. We also discuss the involvement of R loops in cancer development and their potential use as diagnostic biomarkers. Knowledge of the molecular mechanisms underlying R loop dysregulation may significantly improve our understanding of cancer biology and provide new directions for research.","PeriodicalId":12187,"journal":{"name":"FEBS Open Bio","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143700111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hydrophobicity causes anomalous migration of cystine/glutamate antiporter SLC7A11 in SDS-PAGE with low acrylamide concentration. 疏水性导致胱氨酸/谷氨酸反转运蛋白SLC7A11在低丙烯酰胺浓度的SDS-PAGE中异常迁移。

IF 2.8 4区生物学

FEBS Open Bio Pub Date : 2025-03-24 DOI: 10.1002/2211-5463.70019

Nsengiyumva Emmanuel, Qian He, Yixin Kang, Dianbao Zhang, Min Gao, Minglin Wang, Kexin Fan, Jingwen Xiong, Shaobo Wu, Botao Fa, Zhengtao Xiao, Yingfang Niu, Jun Yao, Yilei Zhang

{"title":"Hydrophobicity causes anomalous migration of cystine/glutamate antiporter SLC7A11 in SDS-PAGE with low acrylamide concentration.","authors":"Nsengiyumva Emmanuel, Qian He, Yixin Kang, Dianbao Zhang, Min Gao, Minglin Wang, Kexin Fan, Jingwen Xiong, Shaobo Wu, Botao Fa, Zhengtao Xiao, Yingfang Niu, Jun Yao, Yilei Zhang","doi":"10.1002/2211-5463.70019","DOIUrl":"https://doi.org/10.1002/2211-5463.70019","url":null,"abstract":"The cystine/glutamate antiporter, solute carrier family 7 member 11 (SLC7A11), plays a crucial role in regulating redox homeostasis and cell death processes such as apoptosis and ferroptosis. These processes are implicated in various diseases, including cancer, organ injuries and neurodegenerative disorders. However, the sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) expression pattern of SLC7A11 varies across studies and remains unclear. In many studies, including ours, SLC7A11 migrates at an atypical molecular weight (MW) of approximately 37 kDa, which is lower than its theoretical molecular mass of 55.4 kDa. This discrepancy raises concerns about the precise molecular mass and expression pattern of SLC7A11 in SDS-PAGE. We confirmed that this fast-migrating band corresponds to SLC7A11 through knockdown of endogenous SLC7A11 or overexpression of exogenous SLC7A11. Furthermore, we ruled out the possibility of proteolytic cleavage after protein translation. We found that the high hydrophobicity of SLC7A11 is a key factor responsible for its anomalous migration. Substituting the non-polar residue isoleucine (Ile) with the polar residue asparagine (Asn) reduced its hydrophobicity and restored normal migration, aligning with its predicted MW of 55 kDa. Additionally, we observed that SLC7A11 migrated faster in SDS-PAGE at lower acrylamide concentrations, whereas higher concentrations (e.g. 12% or 15%) eliminated the gel shift. This study clarifies the expression pattern of SLC7A11 in SDS-PAGE and emphasizes the importance of considering physicochemical properties such as hydrophobicity and gel concentration when characterizing membrane proteins like SLC7A11.","PeriodicalId":12187,"journal":{"name":"FEBS Open Bio","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143691437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Equi-MOI ratio for rapid baculovirus-mediated multiprotein co-expression in insect cells integrating selenomethionine for structural studies 在昆虫细胞中以等MOI比例快速表达杆状病毒介导的多蛋白共表达，将硒蛋氨酸用于结构研究。

IF 2.8 4区生物学

FEBS Open Bio Pub Date : 2025-03-18 DOI: 10.1002/2211-5463.70025

Andrej Bitala, Mário Benko, Marek Nemčovič, Ivana Nemčovičová

{"title":"Equi-MOI ratio for rapid baculovirus-mediated multiprotein co-expression in insect cells integrating selenomethionine for structural studies","authors":"Andrej Bitala, Mário Benko, Marek Nemčovič, Ivana Nemčovičová","doi":"10.1002/2211-5463.70025","DOIUrl":"10.1002/2211-5463.70025","url":null,"abstract":"Proteins often co-exist as multicomponent assemblies, making their co-expression essential in recombinant production processes. The baculovirus expression vector system is commonly used to produce recombinant multiprotein complexes mostly for structural and functional studies. Although AI-enhanced tools, such as AlphaFold, have revolutionized protein structure prediction, solving the phase problem remains the most significant challenge in X-ray crystallography for determining entirely novel, dynamic, or complex protein structures. To address this challenge, the early incorporation of selenomethionine into native proteins during production is especially advantageous for facilitating experimental phasing. Here, we describe a fast, effective, and versatile research protocol that uniquely combines these two challenging features. The principle of this method is based on using co-infection of several recombinant baculoviruses in so-called equal multiplicity of infection (MOI) or equi-MOI ratio, while at the same time, the balanced selenomethionine incorporation takes place to allow for an accelerated workflow. The delicate balance between individual conditions for producing selenomethionine-incorporated multiprotein complexes with high efficiency has been developed over several years of studying protein complexes; therefore, many useful tips and tricks are provided as well. Moreover, this protocol is straightforward to implement in any wet lab.","PeriodicalId":12187,"journal":{"name":"FEBS Open Bio","volume":"15 4","pages":"563-572"},"PeriodicalIF":2.8,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/2211-5463.70025","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143656651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

METTL3 knockout accelerates hepatocarcinogenesis via inhibiting endoplasmic reticulum stress response. METTL3基因敲除通过抑制内质网应激反应加速肝癌的发生。

IF 2.8 4区生物学

FEBS Open Bio Pub Date : 2025-03-18 DOI: 10.1002/2211-5463.70023

Bo Cui, Silin Tu, Haibo Li, Zhancheng Zeng, Ruiqi Xiao, Jing Guo, Xiaoqi Liang, Chang Liu, Lijie Pan, Wenjie Chen, Mian Ge, Xiaofen Zhong, Linsen Ye, Huaxin Chen, Qi Zhang, Yan Xu

{"title":"METTL3 knockout accelerates hepatocarcinogenesis via inhibiting endoplasmic reticulum stress response.","authors":"Bo Cui, Silin Tu, Haibo Li, Zhancheng Zeng, Ruiqi Xiao, Jing Guo, Xiaoqi Liang, Chang Liu, Lijie Pan, Wenjie Chen, Mian Ge, Xiaofen Zhong, Linsen Ye, Huaxin Chen, Qi Zhang, Yan Xu","doi":"10.1002/2211-5463.70023","DOIUrl":"https://doi.org/10.1002/2211-5463.70023","url":null,"abstract":"Hepatocellular carcinoma (HCC) is among the most common causes of cancer-related deaths worldwide. Previous studies showed that N6-methyladenosine (m6A), the most abundant chemical modification in eukaryotic RNAs, is implicated in HCC progression. Using liver-specific conditional knockout mice, we found that the loss of METTL3, the core catalytic subunit of m6A methyltransferase, significantly promoted hepatic tumor initiation under various oncogenic challenges, contrary to the previously reported oncogenic role of METTL3 in liver cancer cell lines or xenograft models. Mechanistically, we hypothesized that METTL3 deficiency accelerated HCC initiation by inhibiting m6A deposition on MANF transcripts, impairing nuclear export and thus MANF protein levels, which led to insufficient endoplasmic reticulum (ER) stress response pathway activation. Our findings suggest a tumor-suppressive role for METTL3 in the early stages of HCC, emphasizing the importance of understanding the dynamic role of epigenetic regulation in tumorigenesis and targeted therapy.","PeriodicalId":12187,"journal":{"name":"FEBS Open Bio","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143656720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An enzyme-linked immunosorbent assay (ELISA)-based activity assay for AMP-activated protein kinase (AMPK). 基于酶联免疫吸附试验（ELISA）的amp活化蛋白激酶（AMPK）活性测定。

IF 2.8 4区生物学

FEBS Open Bio Pub Date : 2025-03-11 DOI: 10.1002/2211-5463.70017

Trezze P Nguyen, Shangze Lyu, Yang Liu

引用次数: 0

Dysfunctional tetraspanin 7 (TSP-7) in Caenorhabditis elegans promotes; increases in average life- & health-span, stress-induced survival and motility. 功能失调的四联蛋白7 （TSP-7）在秀丽隐杆线虫中促进；增加平均寿命和健康寿命，压力诱发的生存和运动能力。

IF 2.8 4区生物学

FEBS Open Bio Pub Date : 2025-03-10 DOI: 10.1002/2211-5463.70013

Brogan Jones, Laurence Seabra, Francesco Michelangeli

{"title":"Dysfunctional tetraspanin 7 (TSP-7) in Caenorhabditis elegans promotes; increases in average life- & health-span, stress-induced survival and motility.","authors":"Brogan Jones, Laurence Seabra, Francesco Michelangeli","doi":"10.1002/2211-5463.70013","DOIUrl":"https://doi.org/10.1002/2211-5463.70013","url":null,"abstract":"Caenorhabditis elegans (C. elegans) tetraspanin-7 (TSP-7) protein is an orthologue of the Human tetraspanin CD63, which has recently been shown to be a negative regulator of autophagy. In this study a mutant strain of wild-type (WT) C. elegans (tm5761) with a 352 bp deletion in the tsp-7 gene, was studied. A polyclonal antibody was raised to a peptide sequence present only in the wild-type strain (N2). This antibody cross-reacted with the protein of the correct molecular weight (MW) in the WT lysate, but not in the tm5761, confirming the absence of a functional TSP-7 in this strain. From life-span studies, the tm5761 strain had a higher average survival age of 23.3 ± 0.6, compared to 20.1 ± 0.8 days for WT, although the absolute life-span was not statistically different. This indicates that the mutant tm5761 strain has an increased physiological health-span. Survival studies undertaken at 37 °C, showed a decrease in survival levels, with complete death of the WT occurring after 3 h of exposure, whereas the tm5761 strain was more robust (i.e. 25% survival after 3 h). Sub-lethal osmotic stress caused by increased sodium chloride (NaCl) concentrations was investigated by observing stress-related motility, such as frequency of coiling and reversing. These results showed that the tm5761 strain was more motile at higher concentrations of NaCl than the WT. These findings suggest that, like CD63, TSP-7 could be acting as a negative regulator of autophagy; therefore, the tm5761 strain likely has increased basal autophagy. This would explain its; increased, mean life- and health-span, motility under stress, and improved thermotolerance.","PeriodicalId":12187,"journal":{"name":"FEBS Open Bio","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143585300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Establishment and biological characterization of radioresistant colorectal cancer cell lines. 结直肠癌放射耐药细胞系的建立及生物学特性研究。

IF 2.8 4区生物学

FEBS Open Bio Pub Date : 2025-03-08 DOI: 10.1002/2211-5463.70016

Tian-Yin Qu, Qing Dai, Jing Leng, Lin Fang, Jing-Jing Ma, Rui Chen, Che Chen, Peng-Fei Ran, Wen-Wen Zhou, Chang Liu, Huang-Fei Yu

{"title":"Establishment and biological characterization of radioresistant colorectal cancer cell lines.","authors":"Tian-Yin Qu, Qing Dai, Jing Leng, Lin Fang, Jing-Jing Ma, Rui Chen, Che Chen, Peng-Fei Ran, Wen-Wen Zhou, Chang Liu, Huang-Fei Yu","doi":"10.1002/2211-5463.70016","DOIUrl":"https://doi.org/10.1002/2211-5463.70016","url":null,"abstract":"Radiotherapy resistance is a major cause of recurrence and metastasis in colorectal cancer (CRC). We established radiotherapy-resistant cell lines to explore the molecular mechanisms of radiotherapy resistance in CRC. HT29 and HCT116 cells were subjected to repeated irradiation at 2 Gy to establish these lines. CCK-8 assay, colony formation, and xenograft tumor experiments were used to detect the radiosensitivity of the cells. DNA damage repair proteins, GSH content, and intracellular ROS were also assayed in parental and resistant cells. We successfully established HT29R and HCT116R radioresistant cell lines after fractionated irradiation, and the cells showed significant tolerance to further irradiation compared with the parental cells and a stronger capacity for DNA damage repair. Meanwhile, ionizing radiation significantly reduced GSH in HT29 and HCT116 parental cells but had no effect on GSH content in resistant cells. These results demonstrate that radioresistant colorectal cancer cell lines were successfully established by the method of continuous irradiation with 2 Gy. This provides a basis for further exploration of the mechanism of colorectal cancer radiotherapy resistance.","PeriodicalId":12187,"journal":{"name":"FEBS Open Bio","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143585341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancing transporter activity in heterologous expression systems with SAHA: a 2500-times more potent and odorless alternative to butyrate. 用SAHA增强异种表达系统中的转运蛋白活性：一种比丁酸盐强2500倍且无味的替代品。

IF 2.8 4区生物学

FEBS Open Bio Pub Date : 2025-03-07 DOI: 10.1002/2211-5463.70015

Svenja Flögel, Maurice Tust, Samira Boussettaoui, Dietmar Fischer, Dirk Gründemann

{"title":"Enhancing transporter activity in heterologous expression systems with SAHA: a 2500-times more potent and odorless alternative to butyrate.","authors":"Svenja Flögel, Maurice Tust, Samira Boussettaoui, Dietmar Fischer, Dirk Gründemann","doi":"10.1002/2211-5463.70015","DOIUrl":"https://doi.org/10.1002/2211-5463.70015","url":null,"abstract":"The functional characterization of plasma membrane transport proteins often relies on their heterologous expression in cultured cells. However, some transporters exhibit low activity, hindering meaningful functional assays. Heterologous expression is usually based on strong viral promoters which in living cells are prone to promoter silencing, a major problem. Here, we investigated the efficacy of low-cost histone deacetylase (HDAC) inhibitors in enhancing transporter activity, comparing the established sodium butyrate (the sodium salt of butyric acid) with valproate/valproic acid (VPA) and suberoylanilide hydroxamic acid (SAHA, also known as vorinostat). Using 293 cells stably transfected with pEBTet plasmids containing the CMV promotor to express the transporters SLC16A9, SLC22A15, and OATP1A2, we measured substrate efflux or uptake via LC-MS/MS following overnight preincubation with the HDAC inhibitors. All three compounds markedly stimulated transporter activity. VPA was less effective than butyrate but still surpassed control conditions. SAHA was cytotoxic at 6 μm, but at 2 μm, the enhancement was consistently comparable to 5 mm butyrate. Additionally, SAHA was more cost-effective and devoid of the repulsive odor characteristic of butyrate. Our findings advocate for replacing butyrate with SAHA to enhance heterologously expressed transporter activity. This offers a more efficient and user-friendly alternative for functional assays.","PeriodicalId":12187,"journal":{"name":"FEBS Open Bio","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143572481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Alkane biosynthesis gene expression and its increased production in recombinant cyanobacteria. 重组蓝藻中烷烃生物合成基因的表达及其产量的增加。

IF 2.8 4区生物学

FEBS Open Bio Pub Date : 2025-03-06 DOI: 10.1002/2211-5463.70009

Misato Nagao, Takato Ozaki, Hirofumi Fukuda, Yu Kanesaki, Munehiko Asayama

{"title":"Alkane biosynthesis gene expression and its increased production in recombinant cyanobacteria.","authors":"Misato Nagao, Takato Ozaki, Hirofumi Fukuda, Yu Kanesaki, Munehiko Asayama","doi":"10.1002/2211-5463.70009","DOIUrl":"https://doi.org/10.1002/2211-5463.70009","url":null,"abstract":"Microalgae such as cyanobacteria convert CO2 to compatible drop-in fuels, such as alkanes. However, the production yield is approximately 0.05-1.0% of the dry weight of natural algae. Here, we aimed to study the role of transcriptional expression, mRNA molecular structure and culture-dependent accumulation of alkanes from two cyanobacteria species. The transcription start sites of the alkane biosynthesis genes ado and aar were identified in the representative cyanobacteria strains Synechocystis sp. PCC 6803 and Limnothrix sp. SK1-2-1, which produce heptadecane and pentadecane, respectively. This characterisation revealed the potential promoters and unique mRNA structures of the ado and aar genes in these species. Transcripts from these genes were induced more in the nitrogen-depleted BG11 (BG11-N) culture than in the BG11 culture, although the biomass was reduced, and as such the amount of alkanes obtained per unit medium was greater for BG11 than for BG11-N. PCC 6803 transconjugants carrying alkane biosynthesis genes from PCC 6803 or SK1-2-1 showed an approximately 1.8- to 2.3-fold increase in heptadecane production compared to the control strain when grown on BG11 cultures without any nitrogen depletion. These results suggest that not only the enzymes ADO/AAR but also the intracellular production of fatty acyl-ACP substrates may be important for the mass production of target alkanes.","PeriodicalId":12187,"journal":{"name":"FEBS Open Bio","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143566535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0