FEBS Open Bio最新文献_第9页

Upregulation of HCN2 in ventral tegmental area is involved in morphine-induced conditioned place preference in rats 大鼠腹侧被盖区 HCN2 的上调与吗啡诱导的条件性位置偏好有关

IF 2.8 4区生物学

FEBS Open Bio Pub Date : 2024-09-12 DOI: 10.1002/2211-5463.13888

Jie Yin, Yang Li, Dan Li, Chenxu Chang, Xiechuan Weng

{"title":"Upregulation of HCN2 in ventral tegmental area is involved in morphine-induced conditioned place preference in rats","authors":"Jie Yin, Yang Li, Dan Li, Chenxu Chang, Xiechuan Weng","doi":"10.1002/2211-5463.13888","DOIUrl":"10.1002/2211-5463.13888","url":null,"abstract":"Morphine is an opioid commonly used to treat pain in clinic, but it also has the potential to be highly addictive, which can lead to abuse. Despite these known risks, the cellular and molecular mechanism of morphine conditioned place preference (CPP) is still unclear. In this study, using a rat model of chronic morphine administration, we found that compared with the control group, the mRNA and protein expression of HCN2 channel in the ventral tegmental area (VTA) were upregulated. Further immunofluorescence analysis showed that the fluorescence intensity of HCN2 channel of VTA dopaminergic neurons in morphine group was significantly enhanced, while the patch clamp recording of brain slices showed that both the magnitude and the density of Ih (HCN channel current) of VTA neurons were significantly increased. Moreover, intra-VTA infusion of ZD7288, a selective inhibitor of HCN channel, into rats of the morphine group decreased morphine CPP. Taken together, our results show that chronic morphine administration induces an upregulation of HCN2 in VTA dopamine neurons, while HCN inhibition reduces morphine CPP, suggesting that HCN channel may be a potential target for the treatment of morphine addiction.","PeriodicalId":12187,"journal":{"name":"FEBS Open Bio","volume":"14 12","pages":"1996-2005"},"PeriodicalIF":2.8,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/2211-5463.13888","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142254008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identifying adeno-associated virus (AAV) vectors that efficiently target high grade glioma cells, for in vitro monitoring of temporal cell responses 确定有效靶向高级别胶质瘤细胞的腺相关病毒（AAV）载体，用于体外监测时间性细胞反应

IF 2.8 4区生物学

FEBS Open Bio Pub Date : 2024-09-10 DOI: 10.1002/2211-5463.13894

Farhana A. Sarker, Yuyan Chen, Adrian Westhaus, Leszek Lisowski, Geraldine M. O'Neill

{"title":"Identifying adeno-associated virus (AAV) vectors that efficiently target high grade glioma cells, for in vitro monitoring of temporal cell responses","authors":"Farhana A. Sarker, Yuyan Chen, Adrian Westhaus, Leszek Lisowski, Geraldine M. O'Neill","doi":"10.1002/2211-5463.13894","DOIUrl":"10.1002/2211-5463.13894","url":null,"abstract":"To improve the translation of preclinical cancer research data to successful clinical effect, there is an increasing focus on the use of primary patient-derived cancer cells with limited growth in culture to reduce genetic and phenotype drift. However, these primary lines are less amenable to standardly used methods of exogenous DNA introduction. Adeno-associated viral (AAV) vectors display tropism for a wide range of human tissues, avidly infect primary cells and have a good safety profile. In the present study, we therefore used a next-generation sequencing (NGS) barcoded AAV screening method to assess transduction capability of a panel of 36 AAVs in primary cell lines representing high-grade glioma (HGG) brain tumours including glioblastoma (GBM) and diffuse intrinsic pontine glioma (DIPG)/diffuse midline glioma (DMG). As proof of principle, we created a reporter construct to analyse activity of the transcriptional co-activators yes-associated protein (YAP) and transcriptional co-activator with PDZ-binding motif (TAZ). Transcriptional activation was monitored by promoter-driven expression of the Timer fluorescent tag, a protein that fluoresces green immediately after transcription and transitions to red fluorescence over time. As expected, attempts to express the reporter in primary HGG cells from plasmid expression vectors were unsuccessful. Using the top candidate from the AAV screen, we demonstrate successful AAV-mediated transduction of HGG cells with the YAP/TAZ dynamic activity reporter. In summary, the NGS-screening approach facilitated screening of many potential AAVs, identifying vectors that can be used to study the biology of primary HGG cells.","PeriodicalId":12187,"journal":{"name":"FEBS Open Bio","volume":"14 11","pages":"1914-1925"},"PeriodicalIF":2.8,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/2211-5463.13894","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142214890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microbiological investigation of pregnancies following vaginal radical trachelectomy using 16S rRNA sequencing of FFPE placental specimens 使用 FFPE 胎盘标本的 16S rRNA 测序对阴道根治性气管切除术后的妊娠进行微生物学调查。

IF 2.8 4区生物学

FEBS Open Bio Pub Date : 2024-09-08 DOI: 10.1002/2211-5463.13892

Risa Tsunematsu, Tasuku Mariya, Mina Umemoto, Shiori Ogawa, Wataru Arai, Suguru E. Tanaka, Kyota Ashikawa, Terufumi Kubo, Yoshiyuki Sakuraba, Tsuyoshi Baba, Shinichi Ishioka, Toshiaki Endo, Tsuyoshi Saito

{"title":"Microbiological investigation of pregnancies following vaginal radical trachelectomy using 16S rRNA sequencing of FFPE placental specimens","authors":"Risa Tsunematsu, Tasuku Mariya, Mina Umemoto, Shiori Ogawa, Wataru Arai, Suguru E. Tanaka, Kyota Ashikawa, Terufumi Kubo, Yoshiyuki Sakuraba, Tsuyoshi Baba, Shinichi Ishioka, Toshiaki Endo, Tsuyoshi Saito","doi":"10.1002/2211-5463.13892","DOIUrl":"10.1002/2211-5463.13892","url":null,"abstract":"This study examined the risk of intrauterine infection associated with radical trachelectomy (RT) in early-stage cervical cancer patients. This procedure preserves fertility but is linked to increased risk of intrauterine infection due to cervical defects during pregnancy. DNA was extracted from the formalin-fixed paraffin-embedded (FFPE) placental specimens of 23 pregnant post-RT patients and 16S rRNA gene sequencing was used for bacterial identification. The prevalence of Lactobacillus crispatus and Burkholderia stabilis was significantly higher in the non-chorioamnionitis group. In contrast, alpha diversity analysis using the PD index showed significantly higher diversity in the chorioamnionitis group (P = 0.04). The demonstrated relationship between chorioamnionitis and microbial diversity affirms the importance of controlling the genital bacterial flora in pregnancies following RT.","PeriodicalId":12187,"journal":{"name":"FEBS Open Bio","volume":"14 11","pages":"1825-1836"},"PeriodicalIF":2.8,"publicationDate":"2024-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/2211-5463.13892","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142153520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Environmental toxicology: how pervasive organic environmental pollutants cause toxicity at the molecular, cellular and organism level 环境毒理学：无处不在的有机环境污染物如何在分子、细胞和生物体层面产生毒性

IF 2.8 4区生物学

FEBS Open Bio Pub Date : 2024-09-06 DOI: 10.1002/2211-5463.13883

Francesco Michelangeli

引用次数: 0

The separation between mRNA-ends is more variable than expected mRNA 末端之间的分隔比预期的更多变。

IF 2.8 4区生物学

FEBS Open Bio Pub Date : 2024-09-03 DOI: 10.1002/2211-5463.13877

Nancy Gerling, J. Alfredo Mendez, Eduardo Gomez, Jaime Ruiz-Garcia

{"title":"The separation between mRNA-ends is more variable than expected","authors":"Nancy Gerling, J. Alfredo Mendez, Eduardo Gomez, Jaime Ruiz-Garcia","doi":"10.1002/2211-5463.13877","DOIUrl":"10.1002/2211-5463.13877","url":null,"abstract":"Effective circularization of mRNA molecules is a key step for the efficient initiation of translation. Research has shown that the intrinsic separation of the ends of mRNA molecules is rather small, suggesting that intramolecular arrangements could provide this effective circularization. Considering that the innate proximity of RNA ends might have important unknown biological implications, we aimed to determine whether the close proximity of the ends of mRNA molecules is a conserved feature across organisms and gain further insights into the functional effects of the proximity of RNA ends. To do so, we studied the secondary structure of 274 full native mRNA molecules from 17 different organisms to calculate the contour length (CL) of the external loop as an index of their end-to-end separation. Our computational predictions show bigger variations (from 0.59 to 31.8 nm) than previously reported and also than those observed in random sequences. Our results suggest that separations larger than 18.5 nm are not favored, whereas short separations could be related to phenotypical stability. Overall, our work implies the existence of a biological mechanism responsible for the increase in the observed variability, suggesting that the CL features of the exterior loop could be relevant for the initiation of translation and that a short CL could contribute to the stability of phenotypes.","PeriodicalId":12187,"journal":{"name":"FEBS Open Bio","volume":"14 12","pages":"1985-1995"},"PeriodicalIF":2.8,"publicationDate":"2024-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/2211-5463.13877","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142125237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optogenetic control of early embryos labeling using photoactivatable Cre recombinase 3.0 利用可光激活的 Cre 重组酶 3.0 对早期胚胎标记进行光遗传控制。

IF 2.8 4区生物学

FEBS Open Bio Pub Date : 2024-09-02 DOI: 10.1002/2211-5463.13862

Kumi Morikawa, Akira Nagasaki, Lue Sun, Eihachiro Kawase, Tatsuhiko Ebihara, Yasuaki Shirayoshi

{"title":"Optogenetic control of early embryos labeling using photoactivatable Cre recombinase 3.0","authors":"Kumi Morikawa, Akira Nagasaki, Lue Sun, Eihachiro Kawase, Tatsuhiko Ebihara, Yasuaki Shirayoshi","doi":"10.1002/2211-5463.13862","DOIUrl":"10.1002/2211-5463.13862","url":null,"abstract":"Establishing a highly efficient photoactivatable Cre recombinase PA-Cre3.0 can allow spatiotemporal control of Cre recombinase activity. This technique may help to elucidate cell lineages, as well as facilitate gene and cell function analysis during development. This study examined the blue light-mediated optical regulation of Cre-loxP recombination using PA-Cre3.0 transgenic early mouse pre-implantation embryos. We found that inducing PA-Cre3.0 expression in the heterozygous state did not show detectable recombination activation with blue light. Conversely, in homozygous embryos, DNA recombination by PA-Cre3.0 was successfully induced by blue light and resulted in the activation of the red fluorescent protein reporter gene, while almost no leaks of Cre recombination activity were detected in embryos without light illumination. Thus, we characterize the conditions under which the PA-Cre3.0 system functions efficiently in early mouse embryos. These results are expected to provide a new optogenetic tool for certain biological studies, such as developmental process analysis and lineage tracing in early mouse embryos.","PeriodicalId":12187,"journal":{"name":"FEBS Open Bio","volume":"14 11","pages":"1888-1898"},"PeriodicalIF":2.8,"publicationDate":"2024-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/2211-5463.13862","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142119351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolic disrupting chemicals in the intestine: the need for biologically relevant models 肠道中的代谢干扰化学物：需要生物相关模型：斑马鱼：我们能从这种对环境敏感的小鱼身上学到什么？

IF 2.8 4区生物学

FEBS Open Bio Pub Date : 2024-09-01 DOI: 10.1002/2211-5463.13878

Chedi Erradhouani, Sylvie Bortoli, Selim Aït-Aïssa, Xavier Coumoul, François Brion

{"title":"Metabolic disrupting chemicals in the intestine: the need for biologically relevant models","authors":"Chedi Erradhouani, Sylvie Bortoli, Selim Aït-Aïssa, Xavier Coumoul, François Brion","doi":"10.1002/2211-5463.13878","DOIUrl":"10.1002/2211-5463.13878","url":null,"abstract":"Although the concept of endocrine disruptors first appeared almost 30 years ago, the relatively recent involvement of these substances in the etiology of metabolic pathologies (obesity, diabetes, hepatic steatosis, etc.) has given rise to the concept of Metabolic Disrupting Chemicals (MDCs). Organs such as the liver and adipose tissue have been well studied in the context of metabolic disruption by these substances. The intestine, however, has been relatively unexplored despite its close link with these organs. In vivo models are useful for the study of the effects of MDCs in the intestine and, in addition, allow investigations into interactions with the rest of the organism. In the latter respect, the zebrafish is an animal model which is used increasingly for the characterization of endocrine disruptors and its use as a model for assessing effects on the intestine will, no doubt, expand. This review aims to highlight the importance of the intestine in metabolism and present the zebrafish as a relevant alternative model for investigating the effect of pollutants in the intestine by focusing, in particular, on cytochrome P450 3A (CYP3A), one of the major molecular players in endogenous and MDCs metabolism in the gut.","PeriodicalId":12187,"journal":{"name":"FEBS Open Bio","volume":"14 9","pages":"1397-1419"},"PeriodicalIF":2.8,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/2211-5463.13878","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142105872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MicroRNAs implicated in canine diffuse large B-cell lymphoma prognosis 与犬弥漫大 B 细胞淋巴瘤预后有关的微 RNA。

IF 2.8 4区生物学

FEBS Open Bio Pub Date : 2024-09-01 DOI: 10.1002/2211-5463.13887

Nelly O. Elshafie, Michael Gribskov, Nathanael I. Lichti, Ekramy E. Sayedahmed, Michael O. Childress, Andrea Pires dos Santos

{"title":"MicroRNAs implicated in canine diffuse large B-cell lymphoma prognosis","authors":"Nelly O. Elshafie, Michael Gribskov, Nathanael I. Lichti, Ekramy E. Sayedahmed, Michael O. Childress, Andrea Pires dos Santos","doi":"10.1002/2211-5463.13887","DOIUrl":"10.1002/2211-5463.13887","url":null,"abstract":"Diffuse large B-cell lymphoma (DLBCL) is the most prevalent subtype of non-Hodgkin lymphoma (NHL) in domestic dogs, with many similarities to its human counterpart. The progression of the disease is rapid, and treatment must be initiated early to achieve cancer remission and extend life. This study examined the relationship between progression-free survival (PFS) and microRNA (miRNA) expression in dogs with DLBCL. miRNAs are small non-coding RNA molecules that typically regulate gene expression post-transcriptionally. They are involved in several pathophysiological processes, including the growth and progression of cancer. Based on the analysis of small RNA sequencing (sRNA-seq) data, we validated a group of miRNAs in lymph nodes from 44 DLBCL-affected dogs with known outcomes. We used quantitative PCR to quantify their expression and report a specific subset of miRNAs is associated with decreased PFS in dogs with DLBCL. The miR-192-5p and miR-16-5p expression were significantly downregulated in dogs with increased PFS. These results indicate that miRNA profiling may potentially identify dogs with DLBCL that will experience poor outcomes following treatment. Identifying specific miRNAs that correlate with the progression of canine DLBCL could aid the development of individualized treatment regimens for dogs.","PeriodicalId":12187,"journal":{"name":"FEBS Open Bio","volume":"14 11","pages":"1899-1913"},"PeriodicalIF":2.8,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/2211-5463.13887","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142105873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Measuring the capacity of yeast for surface display of cell wall-anchored protein isoforms by using β-lactamase as a reporter enzyme. 利用β-内酰胺酶作为报告酶，测量酵母表面显示细胞壁锚定蛋白同工酶的能力。

IF 2.8 4区生物学

FEBS Open Bio Pub Date : 2024-08-28 DOI: 10.1002/2211-5463.13886

Tea Martinić Cezar, Antonia Paić, Stefani Prekpalaj, Renata Teparić, Bojan Žunar, Igor Stuparević

{"title":"Measuring the capacity of yeast for surface display of cell wall-anchored protein isoforms by using β-lactamase as a reporter enzyme.","authors":"Tea Martinić Cezar, Antonia Paić, Stefani Prekpalaj, Renata Teparić, Bojan Žunar, Igor Stuparević","doi":"10.1002/2211-5463.13886","DOIUrl":"https://doi.org/10.1002/2211-5463.13886","url":null,"abstract":"Yeast surface display is a promising biotechnological tool that uses genetically modified yeast cell wall proteins as anchors for enzymes of interest, thereby transforming yeast cell wall into a living catalytic material. Here, we present a comprehensive protocol for quantifying surface-displayed β-lactamase on the cell wall of model yeast Saccharomyces cerevisiae. We use β-lactamase as a reporter enzyme, which we tagged to be anchored to the cell wall closer to its N or C terminus, through the portion of the Pir2 or Ccw12 cell wall proteins, respectively. The catalytic activity of surface-displayed β-lactamase is assessed by its ability to hydrolyze nitrocefin, which produces a colorimetric change that is quantitatively measured by spectrophotometric analysis at 482 nm. This system enables precise quantification of the potential of S. cerevisiae strains for surface display, continuous real-time monitoring of enzyme activity, and facilitates the study of enzyme kinetics and interactions with inhibitors within the cell's native environment. In addition, the system provides a platform for high-throughput screening of potential β-lactamase inhibitors and can be adapted for the visualization of other enzymes, making it a versatile tool for drug discovery and bioprocess development.","PeriodicalId":12187,"journal":{"name":"FEBS Open Bio","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2024-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142092568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An open chat with… Johannes Herrmann 与......约翰内斯-赫尔曼（Johannes Herrmann）畅谈。

IF 2.8 4区生物学

FEBS Open Bio Pub Date : 2024-08-23 DOI: 10.1002/2211-5463.13879

Ioannis Tsagakis, Johannes Herrmann

引用次数: 0