FEBS Open Bio最新文献_第4页

Boundaries of photosynthesis: adaptations of carbon fixation in extreme environments 光合作用的边界：极端环境下的碳固定适应。

IF 2.8 4区生物学

FEBS Open Bio Pub Date : 2025-05-19 DOI: 10.1002/2211-5463.70047

Pere Aguiló-Nicolau, Concepción Iñiguez, Sebastià Capó-Bauçà, Jeroni Galmés

{"title":"Boundaries of photosynthesis: adaptations of carbon fixation in extreme environments","authors":"Pere Aguiló-Nicolau, Concepción Iñiguez, Sebastià Capó-Bauçà, Jeroni Galmés","doi":"10.1002/2211-5463.70047","DOIUrl":"10.1002/2211-5463.70047","url":null,"abstract":"Extreme environments challenge fundamental pillars of photosynthesis: light capture and carbon fixation. Organisms thriving in extreme conditions, such as high and low temperatures, extreme pH levels, and high salinity, have evolved remarkable adaptive mechanisms allowing them to sustain photosynthesis. Research into these adaptations has expanded our understanding of the limits and evolution of photosynthesis, while also providing promising biotechnological applications. In this review, we explore the adaptations that tolerant and extremophilic photosynthetic organisms have evolved, overcoming these environmental challenges while maintaining photosynthetic functionality. These adaptations include modifications in photosystems and electron transport chain components, the development of photoprotective mechanisms, the use of unique CO2-concentrating mechanisms (CCMs), and fine-tuning of Rubisco's kinetic properties and concentration. Our aim is to provide the basis for future research in extremophile biology while highlighting its applications in biotechnology.","PeriodicalId":12187,"journal":{"name":"FEBS Open Bio","volume":"15 7","pages":"1028-1040"},"PeriodicalIF":2.8,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/2211-5463.70047","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144101575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Knockout of the mitoribosome rescue factors Ict1 or Mtrfr is viable in zebrafish but not mice: compensatory mechanisms underlying each factor's loss. 敲除线粒体拯救因子Ict1或Mtrfr在斑马鱼中是可行的，但在小鼠中不可行：每种因子损失背后的补偿机制。

IF 2.8 4区生物学

FEBS Open Bio Pub Date : 2025-05-16 DOI: 10.1002/2211-5463.70054

Nobukazu Nameki, Chika Tomisawa, Soichiro Hoshino, Hidehiko Shimizu, Masashi Abe, Sho Arai, Kanako Kuwasako, Naoki Asakawa, Yusuke Inoue, Takuro Horii, Izuho Hatada, Masakatsu Watanabe

{"title":"Knockout of the mitoribosome rescue factors Ict1 or Mtrfr is viable in zebrafish but not mice: compensatory mechanisms underlying each factor's loss.","authors":"Nobukazu Nameki, Chika Tomisawa, Soichiro Hoshino, Hidehiko Shimizu, Masashi Abe, Sho Arai, Kanako Kuwasako, Naoki Asakawa, Yusuke Inoue, Takuro Horii, Izuho Hatada, Masakatsu Watanabe","doi":"10.1002/2211-5463.70054","DOIUrl":"https://doi.org/10.1002/2211-5463.70054","url":null,"abstract":"The mitochondrial translation system contains two ribosome rescue factors, ICT1 and MTRFR (C12orf65), which hydrolyze peptidyl-tRNA in stalled ribosomes. ICT1 also functions as a ribosomal protein of the mitochondrial large ribosomal subunit (mtLSU) in mice and humans, and its deletion is lethal. In contrast, MTRFR does not share this role. Although loss-of-function mutations in MTRFR have been linked to human mitochondrial diseases, data on this association in other vertebrates are lacking. Here, attempts to generate Mtrfr knockout mice were unsuccessful. However, knockout zebrafish lines were successfully generated for both ict1 and mtrfr (ict1-/- and mtrfr-/-). Both knockout lines appeared healthy and fertile. ict1-/-, mtrfr-/-, and wild-type adult caudal fin cells showed significant differences in mitochondrial morphology. The ict1 deletion affected the network properties more than the number of individuals and networks, whereas the mtrfr deletion exhibited the opposite effect. Additionally, the survival rates of the knockout line larvae were significantly lower than those of the wild-type larvae under starvation conditions. These results suggest that ict1 and mtrfr are required for survival under specific stress conditions, whereas ict1-/- and mtrfr-/- involve different compensatory mechanisms in response to loss of either factor under nonstress conditions. Ict1 proteins from all teleosts, including zebrafish, lack the N-terminal mtLSU-binding motif found in most metazoans, suggesting that Ict1 does not function as a ribosomal protein in teleosts. Thus, Mtrfr may partially compensate for the loss of Ict1. In conclusion, zebrafish appear to exemplify a limited category of vertebrates capable of enduring genetic abnormalities in ict1 or mtrfr.","PeriodicalId":12187,"journal":{"name":"FEBS Open Bio","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144076763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Profiling the effect of low frequency mechanical vibration on the metabolic and oxidative stress responses of A431 carcinoma. 分析低频机械振动对A431癌代谢和氧化应激反应的影响。

IF 2.8 4区生物学

FEBS Open Bio Pub Date : 2025-05-16 DOI: 10.1002/2211-5463.70055

Wresti L Anggayasti, Chikahiro Imashiro, Takashi Morikura, Shogo Miyata, Akira Funahashi, Kenjiro Takemura

{"title":"Profiling the effect of low frequency mechanical vibration on the metabolic and oxidative stress responses of A431 carcinoma.","authors":"Wresti L Anggayasti, Chikahiro Imashiro, Takashi Morikura, Shogo Miyata, Akira Funahashi, Kenjiro Takemura","doi":"10.1002/2211-5463.70055","DOIUrl":"https://doi.org/10.1002/2211-5463.70055","url":null,"abstract":"Mechanomedicine represents a potential biocompatible method in cancer therapy. In particular, the use of low-frequency mechanical vibration previously proved to trigger apoptosis of the human epidermoid carcinoma A431 cell line. In this study, we further characterized the metabolic and oxidative stress responses triggered by 1 h of 20 Hz mechanical vibration stimulus to A431 prior to cell death. Our results indicate that cell death may be related to the decrease of glucose consumption rate and the higher expression of reactive oxygen species right after mechanical stimulation (0 h). The overexpression of HMGB1 and HSP70 coding genes signified the increase of A431 cell stress. However, HMGB1 and HSP70 expression decreased at 24 h after mechanical vibration, along with the progression of cell death. We also observed cell morphology changes on A431 cells following vibration which might be indicative of A431 death by apoptosis. The emergence of these stress responses suggests that several pathways are connected to promote cancer cell death. The discovery of A431 cellular stress symptoms which lead to apoptotic death may clarify the usefulness of mechanical vibration in cancer treatment as a novel application of biomechanical manipulation.","PeriodicalId":12187,"journal":{"name":"FEBS Open Bio","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144076767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of 4T1 breast cancer mouse model system for preclinical carbonic anhydrase IX studies. 用于临床前碳酸酐酶IX研究的4T1乳腺癌小鼠模型系统的建立。

IF 2.8 4区生物学

FEBS Open Bio Pub Date : 2025-05-15 DOI: 10.1002/2211-5463.70052

Zane Kalniņa, Ilva Liekniņa, Svetlana Koteloviča, Ramona Petrovska, Gediminas Žvinys, Agne Petrosiute, Asta Zubrienė, Matīss Toms Laugalis, Vendija Skeltona, Juris Jansons, Madara Kreishmane, Edita Čapkauskaitė, Daumantas Matulis, Kaspars Tārs

{"title":"Development of 4T1 breast cancer mouse model system for preclinical carbonic anhydrase IX studies.","authors":"Zane Kalniņa, Ilva Liekniņa, Svetlana Koteloviča, Ramona Petrovska, Gediminas Žvinys, Agne Petrosiute, Asta Zubrienė, Matīss Toms Laugalis, Vendija Skeltona, Juris Jansons, Madara Kreishmane, Edita Čapkauskaitė, Daumantas Matulis, Kaspars Tārs","doi":"10.1002/2211-5463.70052","DOIUrl":"https://doi.org/10.1002/2211-5463.70052","url":null,"abstract":"Triple-negative breast cancer (TNBC) is the most aggressive type of breast cancer, for which targeted treatment is currently lacking. Carbonic anhydrase IX (CAIX) is a known cancer target due to its selective overexpression in hypoxia, a hallmark of many solid cancers including TNBC. This study aimed to develop a robust murine TNBC cell line 4T1-based model system that could be used in the comprehensive preclinical evaluation of targeting CAIX. The model is based on the original 4T1 breast cancer cell line and two genetically edited versions of it-one with biallelic CRISPR/Cas9-mediated Car9 inactivation and another with constitutively expressed Car9, thus ensuring negative and positive controls for CAIX production in the model system, respectively. The generated cell lines were validated for CAIX production and characterised functionally in vitro and in vivo after orthotopic implantation in syngeneic BALB/c mice. Results demonstrated significantly reduced primary tumour growth and metastatic progression rates in animals with CAIX-deficient tumours, while the CAIX-expressing tumours had vascularised phenotypes with prominent central areas of coagulative necrosis. The differential CAIX expression levels in the model were preserved during tumour growth in syngeneic mice, as verified by in vivo imaging using a novel high-affinity CAIX-specific near-infrared (NIR) fluorescent imaging probe, GZ22-4. Constitutive overexpression of autologous CAIX did not elicit specific autoantibody responses in vivo, demonstrating the suitability of this model for evaluating the efficacy of anti-CAIX vaccination as a therapeutic strategy. The in vivo study was repeated as an independent experiment and demonstrated good robustness of the developed model.","PeriodicalId":12187,"journal":{"name":"FEBS Open Bio","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144076759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

RETRACTION: Fibronectin Enhances Tumor Metastasis Through B7-H3 in Clear Cell Renal Cell Carcinoma 结论：透明细胞肾细胞癌中纤维连接蛋白通过B7-H3促进肿瘤转移。

IF 2.8 4区生物学

FEBS Open Bio Pub Date : 2025-05-14 DOI: 10.1002/2211-5463.70053

{"title":"RETRACTION: Fibronectin Enhances Tumor Metastasis Through B7-H3 in Clear Cell Renal Cell Carcinoma","authors":"","doi":"10.1002/2211-5463.70053","DOIUrl":"10.1002/2211-5463.70053","url":null,"abstract":"RETRACTION: J. Xie, M. Sun, D. Zhang, C. Chen, S. Lin, and G. Zhang,“Fibronectin Enhances Tumor Metastasis Through B7-H3 in Clear Cell Renal Cell Carcinoma,” FEBS Open Bio 11, no. 11 (2021): 2977-2987, https://doi.org/10.1002/2211-5463.13280.The above article, published online on 25 August 2021 in Wiley Online Library (wileyonlinelibrary.com), has been retracted by agreement between the authors; the journal Editor-in-Chief, Miguel De la Rosa; the Federation of European Biochemical Societies; and John Wiley & Sons Ltd. UK. The retraction has been agreed due to the discovery of duplications in Figures 2C and 5A. The authors originally reported these irregularities to the journal and offered corrected figures to replace the published figures. Upon the journal's analysis of the original image data, additional irregularities were found, including other image duplications with repositioning. Given the extent of the identified issues, the editors have lost confidence in the data presented and consider the conclusions of this manuscript substantially compromised. As a result, the Editor-in-Chief, FEBS Press, and John Wiley and Sons Ltd. have determined that a retraction is necessary. The authors agree with this decision.","PeriodicalId":12187,"journal":{"name":"FEBS Open Bio","volume":"15 6","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/2211-5463.70053","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143999170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The role of circular RNAs in regulating cytokine signaling in cancer. 环状rna在调节肿瘤细胞因子信号传导中的作用。

IF 2.8 4区生物学

FEBS Open Bio Pub Date : 2025-05-12 DOI: 10.1002/2211-5463.70051

Vandana Joshi, Swati, Amit Mishra, Amaresh Panda, Vivek Sharma

引用次数: 0

Iron-dependent lysosomal LDL oxidation induces the expression of scavenger receptor A in human THP-1 monocytes. 铁依赖性溶酶体LDL氧化诱导人THP-1单核细胞清道夫受体A的表达。

IF 2.8 4区生物学

FEBS Open Bio Pub Date : 2025-05-11 DOI: 10.1002/2211-5463.70048

Martina Čierna, Richard Buchal, Martin Leníček, Amit Shachak, Jan Pláteník

{"title":"Iron-dependent lysosomal LDL oxidation induces the expression of scavenger receptor A in human THP-1 monocytes.","authors":"Martina Čierna, Richard Buchal, Martin Leníček, Amit Shachak, Jan Pláteník","doi":"10.1002/2211-5463.70048","DOIUrl":"https://doi.org/10.1002/2211-5463.70048","url":null,"abstract":"Atherosclerosis leading to cardiovascular diseases remains a dominant medical problem. In the early stages of this disease, the interaction between circulating monocytes and the endothelium is crucial. Monocytes and macrophages express scavenger receptor A (SR-A), which mediates cell adhesion and subsequently uptake of oxidized low-density lipoproteins (LDL). High iron stores in monocytes or macrophages are known to predispose individuals to atherosclerosis, however the reasons remain poorly understood. We hypothesized that a combination of iron and LDL may induce proatherogenic changes in circulating monocytes. Here, we treated a human monocytic cell line THP-1 with isolated LDL and/or iron. A limited uptake of native LDL, but not iron or oxidized LDL, markedly induced expression of SR-A in these cells. Both SR-AI and SR-AII isoforms were upregulated. The increased SR-A was also seen at the protein level, and LDL treatment increased cellular adhesion. The induction of SR-A by LDL was inhibited by the lysosomotropic thiol WR-1065 and by the chain-breaking lipophilic antioxidant butylated hydroxytoluene (BHT). The fluorescent probe BODIPY C11 exhibited increased lipid peroxidation inside lysosomes after LDL administration. The induction of SR-A by LDL was blocked by two silencing RNAs directed against the nuclear coactivator receptor NCOA4, the cargo receptor necessary for the autophagy of ferritin. These results may point to a new pathogenetic mechanism of early-stage atherosclerosis, in which high iron stores in circulating monocytes, through increased lysosomal lipid peroxidation, may lead to an upregulated expression of SR-A, which makes the cells more adhesive and hence more atherogenic.","PeriodicalId":12187,"journal":{"name":"FEBS Open Bio","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143992284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An approach for coherent periodogram averaging of tilt-series data for improved contrast transfer function estimation. 倾斜序列数据相干周期图平均的改进对比度传递函数估计方法。

IF 2.8 4区生物学

FEBS Open Bio Pub Date : 2025-05-08 DOI: 10.1002/2211-5463.70050

Sagar Khavnekar, William Wan

{"title":"An approach for coherent periodogram averaging of tilt-series data for improved contrast transfer function estimation.","authors":"Sagar Khavnekar, William Wan","doi":"10.1002/2211-5463.70050","DOIUrl":"https://doi.org/10.1002/2211-5463.70050","url":null,"abstract":"Cryo-electron microscopy (cryo-EM) has become an indispensable technique for determining three-dimensional structures of biological macromolecules. A critical aspect of achieving high-resolution cryo-EM reconstructions is accurately determining and correcting for the microscope's contrast transfer function (CTF). The CTF introduces defocus-dependent distortions during imaging; if not properly accounted for, the CTF can distort features in and limit the resolution of 3D reconstructions. For tilt-series data used in cryo-electron tomography (cryo-ET), CTF estimation becomes even more challenging due to the tilt of the specimen, which introduces a defocus gradient across the field of view, as well as the low dose and signal in individual tilt images. Here, we describe a simple algorithm to improve the accuracy of CTF estimation of tilted images by leveraging the tilt-series alignment parameters determined for tomographic reconstruction to explicitly account for the tilted specimen geometry. In brief, each tilt image is divided into patches, each of which are then stretched according to their defocus shift. These are then summed to provide a coherent power spectrum at the tilt axis, which can then be used in standard CTF estimation algorithms. This uses all the data in each image to enhance the visibility of Thon rings, thereby improving high-resolution CTF estimation and subsequent enhancements in the resolution of subtomogram averages.","PeriodicalId":12187,"journal":{"name":"FEBS Open Bio","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143990795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The impact of frailty syndrome on skeletal muscle histology: preventive effects of exercise. 虚弱综合征对骨骼肌组织学的影响：运动的预防作用。

IF 2.8 4区生物学

FEBS Open Bio Pub Date : 2025-05-05 DOI: 10.1002/2211-5463.70049

Fujue Ji, Hae Sung Lee, Haesung Lee, Jong-Hee Kim

{"title":"The impact of frailty syndrome on skeletal muscle histology: preventive effects of exercise.","authors":"Fujue Ji, Hae Sung Lee, Haesung Lee, Jong-Hee Kim","doi":"10.1002/2211-5463.70049","DOIUrl":"https://doi.org/10.1002/2211-5463.70049","url":null,"abstract":"Frailty syndrome, a condition marked by increased vulnerability due to age-related physiological decline, exerts a profound impact on skeletal muscle structure and function. Despite its widespread prevalence, the underlying mechanisms contributing to frailty-associated muscle deterioration remain poorly elucidated. This study utilized histological and biochemical analyses in a murine model to investigate the effects of frailty syndrome on skeletal muscle. Mice were classified based on age and condition, including a subset subjected to an exercise intervention. Parameters evaluated included body weight, lean mass ratio, myofiber size and number, extracellular matrix (ECM) content, and myosin heavy chain isoform expression. Frailty syndrome led to increased body weight and ECM content, coupled with reductions in myofiber size and number, reflecting substantial structural and functional impairments in skeletal muscle. Exercise interventions effectively countered these deleterious changes, preserving myofiber morphology and reducing ECM expansion, thereby demonstrating the protective role of exercise in mitigating frailty-induced muscle deterioration. The study highlights the severe impact of frailty syndrome on skeletal muscle structure and integrity. Importantly, it underscores the potential of regular exercise as an effective therapeutic approach to prevent or reverse muscle deterioration associated with frailty, offering critical insights into managing age-related muscular degeneration.","PeriodicalId":12187,"journal":{"name":"FEBS Open Bio","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143958487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Neurotoxic amyloid β-peptide and tau produce cytokine-like effects on PMCA in glioblastoma cell lines, enhancing its activity and isoforms expression. 神经毒性淀粉样蛋白β-肽和tau对胶质母细胞瘤细胞系PMCA产生细胞因子样作用，增强其活性和同工型的表达。

IF 2.8 4区生物学

FEBS Open Bio Pub Date : 2025-05-05 DOI: 10.1002/2211-5463.70046

María Berrocal, Alberto Alvarez-Barrientos, Ana M Mata

引用次数: 0