FEBS Open Bio最新文献

{"title":"Using a flipped classroom teaching and learning approach to promote scientific literacy skill development and retention.","authors":"Elaina B K Brendel, Ala Alzubi, Shrujan Rai, Christine Mariathasan, Laelie A Snook, Jennifer M Monk","doi":"10.1002/2211-5463.13938","DOIUrl":"https://doi.org/10.1002/2211-5463.13938","url":null,"abstract":"<p><p>The development of scientific literacy (SL) skills is critical in the life sciences. A flipped classroom reverses traditional learning spaces such that foundational knowledge is acquired by students independently through recorded lectures and/or readings in advance of the lecture period and knowledge is consolidated through active learning activities in the classroom. A flipped classroom learning environment can promote critical skill development and knowledge application, and therefore, could enhance SL skill development. The objectives here were to (a) determine the effect of a flipped classroom learning environment on SL skill development in second-year kinesiology students enrolled in a research methods course and (b) reassess SL skills 4 months later. SL skills were assessed using the validated test of scientific literacy skills (TOSLS) questionnaire at the start and end of the semester (n = 57) and reassessed 4 months later after the summer semester break (n = 46). During the flipped classroom semester, practical SL skills (TOSLS scores) were increased by 16.3% and TOSLS scores were positively correlated with the students' final grade (r = 0.526, P < 0.001). Four months later, average TOSLS scores significantly decreased compared to the levels at the end of the flipped classroom learning experience. Importantly, retention of SL skills (i.e., 4 months later TOSLS scores) were related to learning approach scores and were positively correlated with deep learning approach scores (r = 0.298, P = 0.044) and negatively correlated with surface learning approach scores (r = -0.314, P = 0.034). Therefore, SL skill retention was higher in students utilizing a deep learning approach (e.g., engaged, self-regulation in learning, and seeking a deeper understanding of concepts) and lower in students utilizing a surface learning approach (e.g., limited engagement, rote memorization of concepts). Collectively, the results demonstrate the value of a flipped classroom in promoting SL skills while highlighting the role of students' learning approach in critical skill retention.</p>","PeriodicalId":12187,"journal":{"name":"FEBS Open Bio","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2024-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142766605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potential radiosensitive germline biomarkers in the testes of wild mice after the Fukushima accident","authors":"Syun Tokita,&nbsp;Ryo Nakayama,&nbsp;Yohei Fujishima,&nbsp;Valerie Swee Ting Goh,&nbsp;Donovan Anderson,&nbsp;Ippei Uemura,&nbsp;Hikari Ikema,&nbsp;Jin Shibata,&nbsp;Yoh Kinoshita,&nbsp;Yoshinaka Shimizu,&nbsp;Hisashi Shinoda,&nbsp;Jun Goto,&nbsp;Maria Grazia Palmerini,&nbsp;Abdulla Mohamed Hatha,&nbsp;Takashi Satoh,&nbsp;Akifumi Nakata,&nbsp;Manabu Fukumoto,&nbsp;Tomisato Miura,&nbsp;Hideaki Yamashiro","doi":"10.1002/2211-5463.13927","DOIUrl":"10.1002/2211-5463.13927","url":null,"abstract":"<p>We investigated potential germline-specific radiosensitive biomarkers in the testes of large Japanese field mice (<i>Apodemus speciosus</i>) exposed to low-dose-rate (LDR) radiation after the Fukushima accident. Fukushima wild mice testes were analysed via RNA-sequencing to identify genes differentially expressed in the breeding and non-breeding seasons when compared to controls. Results revealed significant changes during the breeding season, with <i>Lsp1</i> showing a considerable upregulation, while <i>Ptprk</i> and <i>Tspear</i> exhibited significant reductions. Conversely, in the non-breeding season, <i>Fmo2</i> and <i>Fmo2</i> (highly similar) were significantly upregulated in radiation-exposed Fukushima mice. qPCR analysis results were consistent with transcriptome sequencing, detecting <i>Lsp1</i> and <i>Ptprk</i> regulation in the testes of Fukushima mice. While differences in gene expression were observed, these do not imply any causal association between the identified biomarkers and chronic LDR exposure, as other factors such as the environment and developmental age may contribute. This study provides valuable insights into the reproductive biology is affected by environmental radiation and highlights the value of assessing the effects of chronic LDR radiation exposure on testicular health in wild mice.</p>","PeriodicalId":12187,"journal":{"name":"FEBS Open Bio","volume":"15 2","pages":"296-310"},"PeriodicalIF":2.8,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/2211-5463.13927","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142766107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reviewers acknowledgement","authors":"","doi":"10.1002/2211-5463.13937","DOIUrl":"https://doi.org/10.1002/2211-5463.13937","url":null,"abstract":"&lt;p&gt;The editors of &lt;i&gt;FEBS Open Bio&lt;/i&gt; would like to thank all those who have given their time and expertise to review articles submitted for publication in Volume 14. Although &lt;i&gt;FEBS Open Bio&lt;/i&gt; does not seek to judge the importance of submissions, our reviewers carefully scrutinize the experimental design and results of all papers, and challenge authors about their conclusions.&lt;/p&gt;&lt;p&gt;The names of these reviewers are listed below; we apologize if we have inadvertently omitted anyone.&lt;/p&gt;&lt;p&gt;Alexandra Araújo&lt;/p&gt;&lt;p&gt;Jörg Kleeff&lt;/p&gt;&lt;p&gt;Niamh Lynam-Lennon&lt;/p&gt;&lt;p&gt;Mohammad Aarif Siddiqui&lt;/p&gt;&lt;p&gt;Ashraf N. Abdalla&lt;/p&gt;&lt;p&gt;Rita Abranches&lt;/p&gt;&lt;p&gt;Mariana Acuña&lt;/p&gt;&lt;p&gt;Ruchi Agrawal&lt;/p&gt;&lt;p&gt;Rodrigo Aguiar&lt;/p&gt;&lt;p&gt;Javeed Ahmad&lt;/p&gt;&lt;p&gt;Sachiko Akashi-Takamura&lt;/p&gt;&lt;p&gt;Yaaser Q. Almulaiky&lt;/p&gt;&lt;p&gt;Cestmir Altaner&lt;/p&gt;&lt;p&gt;Marcelo Alves Pinto&lt;/p&gt;&lt;p&gt;Franca Anglani&lt;/p&gt;&lt;p&gt;Munehito Arai&lt;/p&gt;&lt;p&gt;Sandra Armstrong&lt;/p&gt;&lt;p&gt;Anoop Arunagiri&lt;/p&gt;&lt;p&gt;Avraham Ashkenazi&lt;/p&gt;&lt;p&gt;Deborah L. Baines&lt;/p&gt;&lt;p&gt;Aria Baniahmad&lt;/p&gt;&lt;p&gt;Christian Barbato&lt;/p&gt;&lt;p&gt;Rebecca Barnes&lt;/p&gt;&lt;p&gt;Francis A. Barr&lt;/p&gt;&lt;p&gt;Kaustuv Basu&lt;/p&gt;&lt;p&gt;Zsófia Bata&lt;/p&gt;&lt;p&gt;Howard Baylis&lt;/p&gt;&lt;p&gt;Susanne Bechstedt&lt;/p&gt;&lt;p&gt;Thomas Becker&lt;/p&gt;&lt;p&gt;Iraldo Bello-Rivero&lt;/p&gt;&lt;p&gt;Alexandre Benedetto&lt;/p&gt;&lt;p&gt;Vitor Bernardes Pinheiro&lt;/p&gt;&lt;p&gt;David Q. Beversdorf&lt;/p&gt;&lt;p&gt;Prasenjit Bhaumik&lt;/p&gt;&lt;p&gt;Sofia-Iris Bibli&lt;/p&gt;&lt;p&gt;Ricardo Biondi&lt;/p&gt;&lt;p&gt;Eric Blair&lt;/p&gt;&lt;p&gt;Jef Boeke&lt;/p&gt;&lt;p&gt;István Bojti&lt;/p&gt;&lt;p&gt;Stephanie Marie Bozonet&lt;/p&gt;&lt;p&gt;Piotr Bragoszewski&lt;/p&gt;&lt;p&gt;Claudio Brancolini&lt;/p&gt;&lt;p&gt;Stefania Brocca&lt;/p&gt;&lt;p&gt;Laura Brunnthaler&lt;/p&gt;&lt;p&gt;Timothy Bugg&lt;/p&gt;&lt;p&gt;Peter Burkovics&lt;/p&gt;&lt;p&gt;C. Joaquin Caceres&lt;/p&gt;&lt;p&gt;Christine Cavazza&lt;/p&gt;&lt;p&gt;Jobichen Chacko&lt;/p&gt;&lt;p&gt;Dibakar Chakrabarty&lt;/p&gt;&lt;p&gt;Joydeep Chakraborty&lt;/p&gt;&lt;p&gt;Sohini Chakraborty&lt;/p&gt;&lt;p&gt;Etienne Challet&lt;/p&gt;&lt;p&gt;Shih-Ching Chao&lt;/p&gt;&lt;p&gt;Huaiyong Chen&lt;/p&gt;&lt;p&gt;Qun Chen&lt;/p&gt;&lt;p&gt;Wanlin Chen&lt;/p&gt;&lt;p&gt;Chun-Jung Chen&lt;/p&gt;&lt;p&gt;Zhe Chi&lt;/p&gt;&lt;p&gt;Jong-Il Choi&lt;/p&gt;&lt;p&gt;Pooi Yin Chung&lt;/p&gt;&lt;p&gt;Carmen Clapp&lt;/p&gt;&lt;p&gt;Rosemary Clyne&lt;/p&gt;&lt;p&gt;Anne Conibear&lt;/p&gt;&lt;p&gt;Valeria Consoli&lt;/p&gt;&lt;p&gt;Anne Cooke&lt;/p&gt;&lt;p&gt;Fasseli Coulibaly&lt;/p&gt;&lt;p&gt;Xavier Coumoul&lt;/p&gt;&lt;p&gt;Marie Couturier&lt;/p&gt;&lt;p&gt;Nancy Craig&lt;/p&gt;&lt;p&gt;Guillaume Croville&lt;/p&gt;&lt;p&gt;Colm Cunningham&lt;/p&gt;&lt;p&gt;Fiona Cuskin&lt;/p&gt;&lt;p&gt;Boris Cvek&lt;/p&gt;&lt;p&gt;Sakda Daduang&lt;/p&gt;&lt;p&gt;Baoying Dai&lt;/p&gt;&lt;p&gt;Xavier Daura&lt;/p&gt;&lt;p&gt;Peter L. Davies&lt;/p&gt;&lt;p&gt;Caitlin Davis&lt;/p&gt;&lt;p&gt;Iñaki de Diego Martinez&lt;/p&gt;&lt;p&gt;Luitzen de Jong&lt;/p&gt;&lt;p&gt;Juan Vladimir de la Rosa Medina&lt;/p&gt;&lt;p&gt;Ermelinda De Meo&lt;/p&gt;&lt;p&gt;Caio de Oliveira Gorgulho Silva&lt;/p&gt;&lt;p&gt;Danya Dean&lt;/p&gt;&lt;p&gt;Christophe Decroos&lt;/p&gt;&lt;p&gt;Jeroen Den Hertog&lt;/p&gt;&lt;p&gt;Sven Dennerlein&lt;/p&gt;&lt;p&gt;Jayne Dennis&lt;/p&gt;&lt;p&gt;Vincenzo Desiderio&lt;/p&gt;&lt;p&gt;Antimo Di Maro&lt;/p&gt;&lt;p&gt;Cecilia Diaz Oreiro&lt;/p&gt;&lt;p&gt;Victoria Diedrich&lt;/p&gt;&lt;p&gt;Richard Dixon&lt;/p&gt;&lt;p&gt;Chaoqing Dong&lt;/p&gt;&lt;p&gt;Eyal Dor-On&lt;/p&gt;&lt;p&gt;Jiten Doshi&lt;/p&gt;&lt;p&gt;Senouwa Dossou&lt;/p&gt;&lt;p&gt;Matthew Drew&lt;/p&gt;&lt;p&gt;Joanna Dulińska-Litewka&lt;/p&gt;&lt;p&gt;Nicolas Dumaz&lt;/p&gt;&lt;p&gt;Katarzyna Patrycja Dzik&lt;/p&gt;&lt;p&gt;Jason Ear&lt;/p&gt;&lt;p&gt;Adrienne Edkins&lt;/p&gt;&lt;p&gt;Sigrun Eick&lt;/p&gt;&lt;p&gt;Andrey Elchaninov&lt;/p&gt;&lt;p&gt;Sherien M. El-Daly&lt;/p&gt;&lt;p&gt;Toshiya Endo&lt;/p&gt;&lt;p&gt;Ferenc Erdődi&lt;/p&gt;&lt;p&gt;Catherine Etchebest&lt;/p&gt;&lt;p&gt;Abdul Samath Ethayathulla&lt;/p&gt;&lt;p&gt;Chukwuebuka Eze&lt;/p&gt;&lt;","PeriodicalId":12187,"journal":{"name":"FEBS Open Bio","volume":"14 12","pages":"2113-2116"},"PeriodicalIF":2.8,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/2211-5463.13937","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142762201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Report on the 23rd FEBS Young Scientists' Forum 2024","authors":"Riccardo Miggiano,&nbsp;Luigi Ippolito,&nbsp;Chiara Paganini,&nbsp;Alessio Paone,&nbsp;Francesca Tonelli,&nbsp;Sonia Trojan,&nbsp;Irene Díaz-Moreno","doi":"10.1002/2211-5463.13933","DOIUrl":"https://doi.org/10.1002/2211-5463.13933","url":null,"abstract":"<p>The 23rd FEBS YSF was held from 26th to 29th June 2024 in Pavia, Italy. Over 100 PhD students and early postdoctoral researchers from around 30 different countries came together at the inspiring rooms of the University of Pavia for a four-day event. This year's topic was ‘Biochemistry for bridging the gap’, meaning the opportunity to have a comprehensive perspective on all biochemistry applications. Four renowned keynote speakers presented their latest research, accompanied by four career-focused speakers, as well as additional sessions on academic career opportunities, including fellowships, women in science, and laboratory sustainability. Additionally, 10 selected YSF participants gave short talks to a large audience, while the remaining attendees shared their research findings through flash talks and two dedicated poster sessions. Scientific exchange and networking were encouraged during the poster sessions, breaks, and the social events. The meeting was a prelude before attending the 48th FEBS congress, celebrated in Milan. The success of the series will be continued during the 24th YSF edition: ‘Inspired by nature, driven by science’, which will take place from 2nd to 5th July 2025 in Sapanca, Türkiye.</p>","PeriodicalId":12187,"journal":{"name":"FEBS Open Bio","volume":"14 12","pages":"1934-1939"},"PeriodicalIF":2.8,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/2211-5463.13933","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142762172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Professor Stuart John Ferguson (29 September 1949–25 April 2024)","authors":"Julie M. Stevens","doi":"10.1002/2211-5463.13943","DOIUrl":"https://doi.org/10.1002/2211-5463.13943","url":null,"abstract":"&lt;p&gt;We reflect with great fondness and admiration on the life and work of Stuart Ferguson, following his passing earlier this year at the age of 74. He was an internationally distinguished scientist who leaves a significant legacy. He was a dedicated long-term member of the Editorial Board of &lt;i&gt;FEBS Letters&lt;/i&gt; and more recently on the Board of &lt;i&gt;FEBS Open Bio&lt;/i&gt;, where he was a founding member and a vital contributor to its formation.&lt;/p&gt;&lt;p&gt;Born in the UK, Stuart attended the University of Oxford as an undergraduate in Chemistry, where he was awarded a first-class degree. He completed his PhD under the supervision of the renowned scientist George Radda, who also passed away recently. Stuart took up a lectureship at the University of Birmingham, where he met and published with Tina George; they later married and had two sons, Robin and George. He returned in 1985 to Oxford to St Edmund Hall, as the William R Miller Tutorial Fellow in Biochemistry. Stuart built a productive multidisciplinary research group in Oxford, with numerous national and international collaborations, and in 1997, he was awarded the title of Professor of Biochemistry. His legacy is marked by exceptional contributions to both research and education in the field of bioenergetics: the very fundamental principles of how energy flows in living systems.&lt;/p&gt;&lt;p&gt;His research discoveries were wide-ranging and impacted many areas of biology. It happens that Stuart's very first publication (of hundreds) was in &lt;i&gt;FEBS Letters&lt;/i&gt; in 1972, an NMR study on lysozyme, as was his second paper on one of his favourite protein complexes, ATP synthase.&lt;/p&gt;&lt;p&gt;ATP synthase is central to bioenergetics as it produces ATP, driven by a gradient of ions across membranes. It is a large, multicomponent complex, the mechanism of which took years to elucidate. Stuart conducted a number of key studies on ATP synthase, including a critical early observation using a chemical modification experiment. Modification of only one of the three ATP synthesising components inhibited the entire complex, showing that the three sites were not operating independently. This experiment underpinned the so-called binding change mechanism, for which Boyer later received a Nobel Prize in Chemistry. With the respect and recognition of the community, Stuart went on to become a thought leader on the subject, notably on P/O ratios, and the intriguing variety of subunits in the c-rings of the ATPases from different organisms, as detailed structural information became available.&lt;/p&gt;&lt;p&gt;The enzymology of the nitrogen cycle was a significant area of study for Stuart's group and the subject of many collaborations for much of his career. The context for Stuart's interest in the bacterium &lt;i&gt;Paracoccus denitrificans&lt;/i&gt; was that it is a close relative of the bacterial progenitor of our own mitochondria (a discovery made nearby in Oxford's Botany Department). Mitochondria are known to be remnants of bacteria that colonised our ancestral ce","PeriodicalId":12187,"journal":{"name":"FEBS Open Bio","volume":"14 12","pages":"1932-1933"},"PeriodicalIF":2.8,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/2211-5463.13943","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142762171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"FXR activation reduces the formation of macrophage foam cells and atherosclerotic plaque, possibly by down regulating hepatic lipase in macrophages","authors":"Qiang Gu,&nbsp;Jia Liu,&nbsp;Li Li Shen","doi":"10.1002/2211-5463.13925","DOIUrl":"10.1002/2211-5463.13925","url":null,"abstract":"<p>Macrophages are the most important immune cells affecting the formation of atherosclerotic plaque. Nevertheless, the mechanisms that promote formation of foamy macrophages during atherogenesis remain poorly understood. This study explored the effects of Farnesoid X receptor (FXR) and hepatic lipase (HL, encoded by <i>LIPC</i>) on atherogenesis, particularly in foamy macrophage formation. A luciferase reporter assay indicated that FXR could bind to the <i>LIPC</i> promoter and inhibit <i>LIPC</i> transcription. FXR agonist GW4064 decreased HL expression, foam cell formation, and increased the expression of FXR downstream genes and polarization to M2 in ox-LDL-induced THP-1 and U937 foam cells. In addition, GW4064 exerted anti-atherosclerotic effects in ApoE^−/− mice, manifested as decreased serum cholesterol and triglyceride levels, and alleviated atherosclerotic plaque formation. Collectively, FXR exerted anti-atherosclerotic effects, possibly by negatively regulating HL expression in macrophages.</p>","PeriodicalId":12187,"journal":{"name":"FEBS Open Bio","volume":"15 2","pages":"311-323"},"PeriodicalIF":2.8,"publicationDate":"2024-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/2211-5463.13925","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142727413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of fibromodulin in myocardial fibrosis in a diabetic cardiomyopathy rat model.","authors":"Xiyan Dai, Fan Yang, Dongping Chen, Lu Yang, Zhihui Dong, Can Chen, Jianmin Xiao","doi":"10.1002/2211-5463.13935","DOIUrl":"https://doi.org/10.1002/2211-5463.13935","url":null,"abstract":"<p><p>Diabetic cardiomyopathy (DCM) is pathologically characterized by excessive deposition of extracellular matrix proteins, leading to myocardial fibrosis. Fibromodulin (Fmod) plays a crucial role in the pathogenesis of fibrotic diseases. However, the role and mechanism of Fmod in DCM-related myocardial fibrosis remain unclear. In the present study, we established a DCM rat model and an in vitro model of rat primary cardiac fibroblasts (RPCFs) exposed to high glucose. We assessed mRNA and protein expression levels of Col1a1, Col3a1, α-SMA and Fmod in both models. Fmod-overexpressing (ov-Fmod) and Fmod-knockdown (si-Fmod) rat cardiac fibroblasts (RCFs) were generated. Subsequently, whole RNA sequencing was conducted on ov-Fmod RCFs. The gene Col15a1 was evaluated in the DCM rat and all cell models. The correlation between plasma levels of Fmod and Col15a1 in DCM rat models was assessed. Transcription and protein levels of Fmod, Col1a1, Col3a1 and α-SMA were significantly elevated in DCM rat hearts and RPCFs. In ov-Fmod RCFs, fibrosis markers were similarly increased, except for Col3a1, which decreased. The Col1a1/Col3a1 ratio was elevated. Conversely, knocking down Fmod yielded opposite results. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses indicated that Fmod participates in multiple fibrosis-related pathways, affecting Col15a1. Expression of Col15a1 was significantly decreased in all models, compared to controls, except in si-Fmod RCFs. Importantly, Col15a1 and Fmod in plasma exhibited an inverse relationship in DCM. In summary, Fmod is implicated in DCM, with Fmod overexpression downregulating Col15a1 and increasing the Col1a1/Col3a1 ratio. This mechanism may influence diastolic heart failure in DCM by modulating myocardial stiffness and elasticity.</p>","PeriodicalId":12187,"journal":{"name":"FEBS Open Bio","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142727416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Alcohol induces α2-6sialo mucin O-glycans that kill U937 macrophages mediated by sialic acid-binding immunoglobulin-like lectin 7 (Siglec 7)","authors":"Pi-Wan Cheng,&nbsp;Vishwanath-Reddy Hothpet,&nbsp;Ganapati Bhat,&nbsp;Kristina Bailey,&nbsp;Lei Li,&nbsp;Derrick R. Samuelson","doi":"10.1002/2211-5463.13919","DOIUrl":"10.1002/2211-5463.13919","url":null,"abstract":"<p>Alcohol misuse increases infections and cancer fatalities, but mechanisms underlying its toxicity are ill-defined. We show that alcohol treatment of human tracheobronchial epithelial cells leads to inactivation of giantin-mediated Golgi targeting of glycosylation enzymes. Loss of core 2 <i>N</i>-acetylglucosaminyltransferase 1, which uses only giantin for Golgi targeting, coupled with shifted targeting of other glycosylation enzymes to Golgi matrix protein 130-Golgi reassembly stacking protein 65, the site normally used by core 1 enzyme, results in loss of sialyl Lewis x and increase of sialyl Lewis a and α2-6sialo mucin O-glycans. The α2-6sialo mucin O-glycans induced by alcohol cause death of U937 macrophages mediated by sialic acid-binding immunoglobulin-like lectin 7. These results provide a mechanistic insight into the cause of the toxic effects of alcohol and might contribute to the development of therapies to alleviate its toxicity.</p>","PeriodicalId":12187,"journal":{"name":"FEBS Open Bio","volume":"15 1","pages":"165-179"},"PeriodicalIF":2.8,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11705458/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142727406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Cordyceps militaris extract containing cordycepin on the adipogenesis and lipolysis of adipocytes","authors":"Kazuya Kusama,&nbsp;Kodai Oka,&nbsp;Yumi Yashiro,&nbsp;Kanoko Yoshida,&nbsp;Hiroaki Miyaoka,&nbsp;Kazuhiro Tamura","doi":"10.1002/2211-5463.13930","DOIUrl":"10.1002/2211-5463.13930","url":null,"abstract":"<p>Obesity, a global health concern, results from an energy imbalance leading to lipid accumulation. In the present study, <i>Cordyceps militaris</i> extract (CM) and its primary component, cordycepin, were investigated to characterize their potential effects on adipogenesis and lipolysis. Treatment with CM or cordycepin reduced lipid droplets and increased hormone-sensitive lipase activation in 3T3-L1 cells. In a diabetic obese mouse model, CM and cordycepin lowered serum low-density lipoprotein/very low-density lipoprotein levels and reduced oxidative stress and cell senescence markers. Thus, cordycepin inhibits preadipocyte differentiation and promotes lipolysis, which may serve as a novel obesity treatment. Further studies, including clinical trials, are required to validate the clinical potential of cordycepin.</p>","PeriodicalId":12187,"journal":{"name":"FEBS Open Bio","volume":"15 2","pages":"335-345"},"PeriodicalIF":2.8,"publicationDate":"2024-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/2211-5463.13930","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142686399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protein crystallization and structure determination at room temperature in the CrystalChip.","authors":"Petr Pachl, Léna Coudray, Romain Vincent, Léa Nilles, Hélène Scheer, Christophe Ritzenthaler, Adéla Fejfarová, Pavlína Řezáčová, Sylvain Engilberge, Claude Sauter","doi":"10.1002/2211-5463.13932","DOIUrl":"https://doi.org/10.1002/2211-5463.13932","url":null,"abstract":"<p><p>The production of high-quality crystals is a key step in crystallography in general, but control of crystallization conditions is even more crucial in serial crystallography, which requires sets of crystals homogeneous in size and diffraction properties. This protocol describes the implementation of a simple and user-friendly microfluidic device that allows both the production of crystals by the counter-diffusion method and their in situ analysis by serial crystallography. As an illustration, the whole procedure is used to determine the crystal structure of three proteins from data collected at room temperature at a synchrotron radiation source.</p>","PeriodicalId":12187,"journal":{"name":"FEBS Open Bio","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2024-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142686401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}