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The solution supramolecular structure of α2 → 8 polysialic acid suggests a structural cause for its low immunogenicity.
IF 3.5 4区 生物学
FEBS Letters Pub Date : 2025-03-18 DOI: 10.1002/1873-3468.70032
Kenneth A Rubinson
{"title":"The solution supramolecular structure of α2 → 8 polysialic acid suggests a structural cause for its low immunogenicity.","authors":"Kenneth A Rubinson","doi":"10.1002/1873-3468.70032","DOIUrl":"https://doi.org/10.1002/1873-3468.70032","url":null,"abstract":"<p><p>α2 → 8 polysialic acid (polySia), the capsular polysaccharide on Neisseria meningitidis serogroup B and Escherichia coli K1, elicits poor immunogenic properties. A solution structure might clarify the cause. From X-ray and neutron small-angle scattering, we find that the solution supramolecular structure of polySia exhibits parallel-chain binding. Also, striking structural changes occur upon the addition of calcium to these solutions but still with the parallel motif. The major histocompatibility complex requires entities in the molecular weight range of 600-3000 Da, but the solution structures determined here argue that in endosomes polySia oligomers of the correct size to be immunologically effective are instead bound up as parallel chains. This is expected to reduce the possible concentrations of the effective oligomers and so reduce the immunogenic capability of α2 → 8 polysialic acid.</p>","PeriodicalId":12142,"journal":{"name":"FEBS Letters","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143656683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
IgG4 and IgG1 undergo common acid-induced compaction into an alternatively folded state.
IF 3.5 4区 生物学
FEBS Letters Pub Date : 2025-03-17 DOI: 10.1002/1873-3468.70031
Hiroshi Imamura, Shinya Honda
{"title":"IgG4 and IgG1 undergo common acid-induced compaction into an alternatively folded state.","authors":"Hiroshi Imamura, Shinya Honda","doi":"10.1002/1873-3468.70031","DOIUrl":"https://doi.org/10.1002/1873-3468.70031","url":null,"abstract":"<p><p>Immunoglobulin G1 (IgG1) antibodies undergo denaturation in acidic conditions, resulting in an alternatively folded state (AFS). The AFS structure is more compact than the native state. However, the prevalence of AFS in other subclasses remains largely unexplored. This study provides evidence that humanized IgG4 can also adopt the AFS structure, as demonstrated through size-exclusion chromatography coupled with small-angle X-ray scattering (SEC-SAXS) analysis. These findings suggest that the anomalous compaction of immunoglobulins G (IgGs) is resilient to variations in sequence and structure among subclasses.</p>","PeriodicalId":12142,"journal":{"name":"FEBS Letters","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143647817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
From cytoplasm to lumen-mapping the free pools of protein subunits of three photosynthetic complexes using quantitative mass spectrometry.
IF 3.5 4区 生物学
FEBS Letters Pub Date : 2025-03-12 DOI: 10.1002/1873-3468.70029
Anna M Williams, Philip J Jackson, Steven M Theg, Terry M Bricker, C Neil Hunter, Haijun Liu
{"title":"From cytoplasm to lumen-mapping the free pools of protein subunits of three photosynthetic complexes using quantitative mass spectrometry.","authors":"Anna M Williams, Philip J Jackson, Steven M Theg, Terry M Bricker, C Neil Hunter, Haijun Liu","doi":"10.1002/1873-3468.70029","DOIUrl":"https://doi.org/10.1002/1873-3468.70029","url":null,"abstract":"<p><p>The phycobilisome (PBS) captures light energy and transfers it to photosystem I (PSI) and photosystem II (PSII). Which and how many copies of protein subunits in PBSs, PSI, and PSII remain unbound in thylakoids are unknown. Here, quantitative mass spectrometry (QMS) was used to quantify substantial pools of free extrinsic subunits of PSII and PSI. Interestingly, the membrane intrinsic PsaL is 3-fold higher than PsaA/B. This scenario complements the static structures of these complexes as revealed by X-ray crystallography and cryo-EM. The ratios of ApcG and photoprotective OCP over PBS indicate a pool of extra ApcG. The 2.5 ratio of CpcG-PBS over CpcL-PBS improves our understanding of these light-harvesting complexes involved in energy capture and photoprotection in cyanobacteria. Impact statement Our study presents the first quantitative inquiry of the free pools of proteins associated with the three major photosynthetic complexes in Synechocystis 6803. This study increases our understanding of the unbound thylakoid proteome, guiding future research into the functions of these proteins, which will facilitate efforts to enhance photosynthetic efficiency.</p>","PeriodicalId":12142,"journal":{"name":"FEBS Letters","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143614054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Leaving science-attrition of biologists in 38 OECD countries.
IF 3.5 4区 生物学
FEBS Letters Pub Date : 2025-03-08 DOI: 10.1002/1873-3468.70028
Marek Kwiek, Lukasz Szymula
{"title":"Leaving science-attrition of biologists in 38 OECD countries.","authors":"Marek Kwiek, Lukasz Szymula","doi":"10.1002/1873-3468.70028","DOIUrl":"https://doi.org/10.1002/1873-3468.70028","url":null,"abstract":"<p><p>We examine biologists leaving science in 38 OECD countries in the past two decades. In a cohort-based and longitudinal fashion, we follow individuals over time, from their first publication (N = 86 178). We examine four disciplines: AGRI (agricultural, biological sciences), BIO (biochemistry, genetics, molecular biology), IMMU (immunology, microbiology), and NEURO (neuroscience). Our Kaplan-Meier survival analysis of BIO shows that 60% of women are still in science after 5 years, 40% after 10 years, and only 20% after 19 years. Women in BIO are 23.26% more likely than men to leave science after 10 years and 39.74% after 19 years. Gender differences increase consistently in later career stages. They are high, but comparing the 2000 and 2010 cohorts, have slightly decreased over time.</p>","PeriodicalId":12142,"journal":{"name":"FEBS Letters","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143585318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Unconventional mRNA processing and degradation pathways for the polycistronic yrzI (spyTA) mRNA in Bacillus subtilis.
IF 3.5 4区 生物学
FEBS Letters Pub Date : 2025-03-08 DOI: 10.1002/1873-3468.70027
Laetitia Gilet, Magali Leroy, Alexandre Maes, Ciarán Condon, Frédérique Braun
{"title":"Unconventional mRNA processing and degradation pathways for the polycistronic yrzI (spyTA) mRNA in Bacillus subtilis.","authors":"Laetitia Gilet, Magali Leroy, Alexandre Maes, Ciarán Condon, Frédérique Braun","doi":"10.1002/1873-3468.70027","DOIUrl":"https://doi.org/10.1002/1873-3468.70027","url":null,"abstract":"<p><p>The ribosome-associated endoribonuclease Rae1 cleaves the Bacillus subtilis yrzI operon mRNA in a translation-dependent manner. This operon encodes up to four small peptides, S1027, YrzI, S1025, and S1024, whose functions are unknown. Here, we identified the function of YrzI and S1025 and deciphered the degradation pathways of the yrzI polycistronic mRNA. We show that YrzI is toxic at high concentrations, but co-expression with S1025 abolishes its toxicity, and that, in the absence of Rae1, S1025 is the major antidote to the YzI toxin. We show that a highly stable mRNA species containing the YrzI and S1025 open reading frames results from endoribonucleolytic cleavage upstream of yrzI followed by the arrest of 5'-exoribonucleolytic processing by ribosomes bound to its exceptionally strong Shine-Dalgarno sequence. Degradation of this mRNA requires either translation-dependent cleavage within S1025 by Rae1 or direct attack from the structured 3'-end by 3'-exoribonucleases. Neither pathway is common for a B. subtilis mRNA.</p>","PeriodicalId":12142,"journal":{"name":"FEBS Letters","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143585376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
G protein-coupled oestrogen receptor regulates branched-chain amino acid metabolism through c-Jun N-terminal kinase.
IF 3.5 4区 生物学
FEBS Letters Pub Date : 2025-03-06 DOI: 10.1002/1873-3468.70030
Anshu Gupta, Prasad Govind Shinde, Sachin Jorvekar, Akash Suresh Humane, Mythri Chandrasekaran, Roshan M Borkar, Sudhagar Selvaraju
{"title":"G protein-coupled oestrogen receptor regulates branched-chain amino acid metabolism through c-Jun N-terminal kinase.","authors":"Anshu Gupta, Prasad Govind Shinde, Sachin Jorvekar, Akash Suresh Humane, Mythri Chandrasekaran, Roshan M Borkar, Sudhagar Selvaraju","doi":"10.1002/1873-3468.70030","DOIUrl":"https://doi.org/10.1002/1873-3468.70030","url":null,"abstract":"<p><p>Branched-chain amino acids (BCAA) are essential requirements for overall protein turnover, signalling and energy balance, and dysregulation of their metabolic pathway has been associated with many pathophysiological events. Despite the importance of BCAA in human health, our understanding of their metabolic regulation is limited. Here, we present evidence that G protein-coupled oestrogen receptor (GPER) activation inhibits the key BCAA metabolic regulatory enzyme branched-chain α-keto acid dehydrogenase complex (BCKDH) by phosphorylating S293. Inhibition of BCKDH results in leucine, isoleucine and valine accumulation in cells. Interestingly, GPER did not alter the levels of the kinase BCKDK and the phosphatase PPM1K, which regulate BCKDH activity, but activated MAPK signalling. Using gene silencing, we identified that JNK intercedes GPER-mediated BCKDH inhibition. Together, our results demonstrate that GPER inhibits BCAA metabolism through JNK signalling.</p>","PeriodicalId":12142,"journal":{"name":"FEBS Letters","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143566409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Changes in importin levels promote nuclear proteasomal degradation of cell cycle-related proteins during THP-1 monocyte-to-macrophage differentiation.
IF 3.5 4区 生物学
FEBS Letters Pub Date : 2025-03-05 DOI: 10.1002/1873-3468.70020
Makoto Kimura, Yutaka Ogawa, Shoko Motohashi, Naoko Imamoto
{"title":"Changes in importin levels promote nuclear proteasomal degradation of cell cycle-related proteins during THP-1 monocyte-to-macrophage differentiation.","authors":"Makoto Kimura, Yutaka Ogawa, Shoko Motohashi, Naoko Imamoto","doi":"10.1002/1873-3468.70020","DOIUrl":"https://doi.org/10.1002/1873-3468.70020","url":null,"abstract":"<p><p>Importin family nucleocytoplasmic transport receptors share thousands of cargo proteins. To elucidate cell regulatory mechanisms via transport regulation, we analyzed the levels of transport receptors by western blotting and quantified the total cellular and nuclear proteins during monocyte-to-macrophage differentiation of THP-1 cells using mass spectrometry. Importin-α1 decreased and importin-α5 increased during the differentiation. Cell cycle-related proteins decreased in both whole cells and nuclei, and proteasome-related proteins increased in the nuclei but not in whole cells. During the differentiation with importin-α1 overexpression, the nuclear levels of some cell division-related proteins recovered, and with importin-α5 knockdown, proteasome assembly factors decreased in the nuclei. In this differentiation, transport receptors reduce unnecessary nuclear proteins by abating import and promoting nuclear proteasomal degradation. This study demonstrates the importance of global nuclear transport control in cell regulation.</p>","PeriodicalId":12142,"journal":{"name":"FEBS Letters","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143556401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Making tau amyloid models in vitro: a crucial and underestimated challenge.
IF 3.5 4区 生物学
FEBS Letters Pub Date : 2025-03-03 DOI: 10.1002/1873-3468.70022
Julien Broc, Clara Piersson, Yann Fichou
{"title":"Making tau amyloid models in vitro: a crucial and underestimated challenge.","authors":"Julien Broc, Clara Piersson, Yann Fichou","doi":"10.1002/1873-3468.70022","DOIUrl":"https://doi.org/10.1002/1873-3468.70022","url":null,"abstract":"<p><p>The protein Tau accumulates in the brain as insoluble deposits in a number of neurodegenerative diseases called tauopathies. In vitro work studying the properties of reconstituted tau assemblies has played a major role in understanding the fundamental mechanisms underlying tauopathies. Yet, in our view, the extent to which tau in vitro models represent the assemblies found in the brain is not sufficiently addressed. Here, we provide an overview of the procedures used to form tau amyloids in vitro and we discuss their similarities and differences with brain deposits. We emphasize that, to date, further work is needed to establish how to form accurate models of tau deposits with recombinant protein, let alone how to represent disease-specific properties that discriminate tauopathies.</p>","PeriodicalId":12142,"journal":{"name":"FEBS Letters","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143540839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The intracellular domain of TLR2 is capable of high-affinity Zn binding: possible outcomes for the receptor activation.
IF 3.5 4区 生物学
FEBS Letters Pub Date : 2025-03-03 DOI: 10.1002/1873-3468.70026
Vladislav A Lushpa, Cong Lin, Irina A Talyzina, Marina V Goncharuk, Eduard V Bocharov, Alexander S Arseniev, Xiaohui Wang, Sergey A Goncharuk, Konstantin S Mineev
{"title":"The intracellular domain of TLR2 is capable of high-affinity Zn binding: possible outcomes for the receptor activation.","authors":"Vladislav A Lushpa, Cong Lin, Irina A Talyzina, Marina V Goncharuk, Eduard V Bocharov, Alexander S Arseniev, Xiaohui Wang, Sergey A Goncharuk, Konstantin S Mineev","doi":"10.1002/1873-3468.70026","DOIUrl":"https://doi.org/10.1002/1873-3468.70026","url":null,"abstract":"<p><p>Toll-like receptors (TLRs) are important players in the innate immune system. Binding of pathogen-related molecules to the extracellular domains of TLRs initiates signalosome assembly, a key event in signal transduction. Despite extensive research on individual receptor domains, the mechanism of signalosome assembly remains unclear. Recent evidence suggests that the intracellular TIR domain of TLR1 binds zinc ions, with cysteines playing a pivotal role in binding and receptor activation. This study explores the zinc-binding ability of the TLR2 TIR domain (TLR2<sub>TIR</sub>). We found that TLR2<sub>TIR</sub> binds zinc with nanomolar affinity through its cysteine residues. Two of them, C673 and C713, are essential for receptor activation. These results suggest that zinc may be involved in the initiation of signalosome assembly.</p>","PeriodicalId":12142,"journal":{"name":"FEBS Letters","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143540790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Taurine promotes glucagon-like peptide-1 secretion in enteroendocrine L cells.
IF 3.5 4区 生物学
FEBS Letters Pub Date : 2025-03-03 DOI: 10.1002/1873-3468.70014
Yuri Osuga, Kazuki Harada, Takuya Yamauchi, Tetsuya Kitaguchi, Masami Yokota Hirai, Mitsuharu Matsumoto, Takashi Tsuboi
{"title":"Taurine promotes glucagon-like peptide-1 secretion in enteroendocrine L cells.","authors":"Yuri Osuga, Kazuki Harada, Takuya Yamauchi, Tetsuya Kitaguchi, Masami Yokota Hirai, Mitsuharu Matsumoto, Takashi Tsuboi","doi":"10.1002/1873-3468.70014","DOIUrl":"https://doi.org/10.1002/1873-3468.70014","url":null,"abstract":"<p><p>Taurine, an amino-sulfonic acid mainly sourced from food, suppresses blood glucose by stimulating insulin secretion from pancreatic β-cells. However, its relationship with glucagon-like peptide-1 (GLP-1) secretion from enteroendocrine L cells is unclear. This study aimed to determine the role of taurine in GLP-1 secretion from L cells. Taurine administration promoted GLP-1 secretion in enteroendocrine L cell line GLUTag cells and increased plasma GLP-1 in mice. Taurine uptake via the taurine transporter increased cytosolic ATP levels, resulting in higher intracellular Ca<sup>2+</sup> concentrations and enhanced GLP-1 secretion through ATP-sensitive K<sup>+</sup> channel closure. These findings may help identify new therapeutic targets for obesity and diet-related disease prevention.</p>","PeriodicalId":12142,"journal":{"name":"FEBS Letters","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143540789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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