FEBS Letters最新文献

RETRACTION: Sialic Acids Acquired by Pseudomonas aeruginosa Are Involved in Reduced Complement Deposition and Siglec Mediated Host-Cell Recognition. 撤回：铜绿假单胞菌获得唾液酸参与补体沉积减少和Siglec介导的宿主细胞识别。

IF 3.5 4区生物学

FEBS Letters Pub Date : 2025-05-27 DOI: 10.1002/1873-3468.70082

{"title":"RETRACTION: Sialic Acids Acquired by Pseudomonas aeruginosa Are Involved in Reduced Complement Deposition and Siglec Mediated Host-Cell Recognition.","authors":"","doi":"10.1002/1873-3468.70082","DOIUrl":"https://doi.org/10.1002/1873-3468.70082","url":null,"abstract":"Retraction: B. Khatua, A. Ghoshal, K. Bhattacharya, C. Mandal, B. Saha, P.R. Crocker, and C. Mandal, \"Sialic Acids Acquired by Pseudomonas Aeruginosa Are Involved in Reduced Complement Deposition and Siglec Mediated Host-Cell Recognition,\" FEBS Letters 584, no. 3 (2010): 555-561, https://doi.org/10.1016/j.febslet.2009.11.087. The above article, published online on 27 November 2009 in Wiley Online Library (wileyonlinelibrary.com), has been retracted by agreement between the journal Editor-in-Chief, Michael Brunner; the Federation of European Biochemical Societies; and John Wiley & Sons Ltd. UK. The retraction has been agreed due to concerns raised by a third party. Further investigation revealed the unattributed duplication of nearly all of the data presented in Figures 1 and 2 from Figures 2 and 3 from a now-retracted article by the same authors (DOI: 10.1128/IAI.01083-08). In addition, there is duplication of data within the MALDI-TOF-MS data in Figure 3b and within the flow cytometry-based binding assay data in Figure 5. These concerns undermine the journal's confidence in the results and conclusions presented. Therefore, the parties agree that the article must be retracted.","PeriodicalId":12142,"journal":{"name":"FEBS Letters","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144157542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mechanisms governing GPCR anterograde transport. 控制GPCR顺行运输的机制。

IF 3.5 4区生物学

FEBS Letters Pub Date : 2025-05-27 DOI: 10.1002/1873-3468.70081

Kevin Assoumou, Sofia Papadogkonaki, Itziar Muneta-Arrate, Miriam Stoeber

{"title":"Mechanisms governing GPCR anterograde transport.","authors":"Kevin Assoumou, Sofia Papadogkonaki, Itziar Muneta-Arrate, Miriam Stoeber","doi":"10.1002/1873-3468.70081","DOIUrl":"https://doi.org/10.1002/1873-3468.70081","url":null,"abstract":"G protein-coupled receptors (GPCRs) constitute the largest family of human membrane proteins. GPCRs recognize diverse extracellular stimuli and activate intracellular signaling cascades that regulate key physiological processes such as neurotransmission and cardiovascular function. The controlled transport of nascent GPCRs from the endoplasmic reticulum (ER) via the Golgi apparatus to the cell surface critically determines the cellular responsiveness to incoming ligands. Here, we present a comprehensive overview of the cellular mechanisms and motif-driven interactions with regulatory proteins that orchestrate GPCR folding, post-translational modifications, and vesicular transport along the secretory pathway. We highlight signaling cues that can modulate the anterograde transport and specialized mechanisms that deliver biosynthetic GPCRs to dendrites and axons in neurons. Furthermore, we discuss that many disease-causing GPCR mutants exhibit aberrant intracellular retention, which can be rescued by pharmacological strategies that stabilize misfolded GPCRs. Finally, we highlight insights into the agonist-driven signaling of biosynthetic GPCRs in secretory organelles. This review covers the complex roles of anterograde transport in controlling GPCR function and emerging possibilities to target the underlying mechanisms in disease.","PeriodicalId":12142,"journal":{"name":"FEBS Letters","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144157540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of molecular mechanisms of CaMKKα/1 oligomerization. CaMKKα/1寡聚分子机制的表征。

IF 3.5 4区生物学

FEBS Letters Pub Date : 2025-05-25 DOI: 10.1002/1873-3468.70078

Shun Uenoyama, Hayato Nitta, Satomi Ohtsuka, Masaki Magari, Futoshi Suizu, Hiroshi Tokumitsu

{"title":"Characterization of molecular mechanisms of CaMKKα/1 oligomerization.","authors":"Shun Uenoyama, Hayato Nitta, Satomi Ohtsuka, Masaki Magari, Futoshi Suizu, Hiroshi Tokumitsu","doi":"10.1002/1873-3468.70078","DOIUrl":"https://doi.org/10.1002/1873-3468.70078","url":null,"abstract":"Calcium/calmodulin-dependent protein kinase kinase (CaMKK) is an activating kinase for calcium/calmodulin-dependent protein kinase type 1 (CaMKI), calcium/calmodulin-dependent protein kinase type IV (CaMKIV), RAC-alpha serine/threonine-protein kinase (PKB), and AMP-activated protein kinase (AMPK) that has been reported to form an active oligomer in cells. Glutathione S-transferase (GST) pulldown assay from the extracts of COS-7 cells expressing GST- and His6-CaMKKα/1 mutants showed that the C-terminal region containing the autoinhibitory and calmodulin (CaM)-binding sequence (residues 438-463) is required for CaMKKα/1 homo-oligomerization. This was confirmed by the fact that the GST-CaMKKα/1 C-terminal domain (residues 435-505) directly interacted with EGFP-CaMKKα/1 residues 435-505 as well as with wild-type CaMKKα/1. Notably, once oligomerized in cells, CaMKKα/1 is neither exchangeable between the oligomeric complexes nor dissociated by Ca2+/CaM binding. These results support stable oligomerization of CaMKK in the cells by intermolecular self-association of its C-terminal region containing a regulatory domain.","PeriodicalId":12142,"journal":{"name":"FEBS Letters","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144141909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Brucella NyxA and NyxB dimerization enhances effector function during infection. 布鲁氏菌NyxA和NyxB二聚体在感染过程中增强了效应剂的功能。

IF 3.5 4区生物学

FEBS Letters Pub Date : 2025-05-21 DOI: 10.1002/1873-3468.70069

Lison Cancade-Veyre, Arthur Louche, Francine C A Gérard, Laurent Terradot, Suzana P Salcedo

引用次数: 0

Structural insights into the binding of human TGIF1 with SMAD2 MH2 domain. 人类TGIF1与SMAD2 MH2结构域结合的结构见解。

IF 3.5 4区生物学

FEBS Letters Pub Date : 2025-05-21 DOI: 10.1002/1873-3468.70073

Heng Zhou, Zheyu Xu, Yue Xiong, Yuanyuan Zhang, Runchen Li, Xiaoling He, Rui Hu, Jiang Zhu, Yunhuang Yang, Maili Liu

引用次数: 0

The thioredoxin-like and one glutaredoxin domain are required to rescue the iron-starvation phenotype of HeLa GLRX3 knock out cells. 硫氧还蛋白样结构域和一个glutaredoxin结构域是拯救HeLa GLRX3敲除细胞的缺铁表型所必需的。

IF 3.5 4区生物学

FEBS Letters Pub Date : 2025-05-21 DOI: 10.1002/1873-3468.70072

Laura Magdalena Jordt, Manuela Gellert, Finja Zelms, Sander Bekeschus, Christopher Horst Lillig

{"title":"The thioredoxin-like and one glutaredoxin domain are required to rescue the iron-starvation phenotype of HeLa GLRX3 knock out cells.","authors":"Laura Magdalena Jordt, Manuela Gellert, Finja Zelms, Sander Bekeschus, Christopher Horst Lillig","doi":"10.1002/1873-3468.70072","DOIUrl":"https://doi.org/10.1002/1873-3468.70072","url":null,"abstract":"Glutaredoxin 3 (Grx3) is a multidomain protein (Trx-GrxA-GrxB) with a Trx-like domain and two Grx domains containing a CGFS motif for binding Fe2S2 clusters. To study the function of these domains, HeLa cells with GLRX3 knockout were generated via CRISPR/Cas. The knockout activated iron-regulatory protein 1, indicating iron starvation due to impaired iron metabolism. Transfection with constructs encoding wild-type or individual domains showed that only the Trx-GrxA construct could rescue the phenotype, matching the effect of full-length Grx3. The specific role of the second Grx domain in human Grx3, absent in simpler eukaryotes such as yeast, remains unclear. While the individual domains are insufficient to rescue the knockout of full-length Grx3, the Trx-GrxA module is functionally critical. Impact statement Glutaredoxin 3 (Grx3) contains a Trx-like domain and two Grx domains. The importance of the domains in higher eukaryotes has not previously been addressed in physiological or cellular contexts. Here, we report GLRX3 knockout results in activation of iron regulatory protein 1, and a Trx-GrxA construct could rescue the phenotype.","PeriodicalId":12142,"journal":{"name":"FEBS Letters","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144119301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fraudulent studies are undermining the reliability of systematic reviews: on the prevalence of problematic images in preclinical depression studies. 欺诈性研究正在破坏系统评价的可靠性：关于临床前抑郁症研究中存在问题的图像的普遍性。

IF 3.5 4区生物学

FEBS Letters Pub Date : 2025-05-21 DOI: 10.1002/1873-3468.70077

Jenny P Berrío, Otto Kalliokoski

引用次数: 0

Binding mechanism of adenylate kinase-specific monobodies. 腺苷酸激酶特异性单体的结合机制。

IF 3.5 4区生物学

FEBS Letters Pub Date : 2025-05-21 DOI: 10.1002/1873-3468.70076

Ibuki Nakamura, Hiroshi Amesaka, Satoshi Nagao, Naoki Orito, Shigeru Negi, Shun-Ichi Tanaka, Takashi Matsuo

引用次数: 0

B cell mechanobiology in health and disease: emerging techniques and insights into therapeutic responses. 健康和疾病中的B细胞机械生物学：新兴技术和对治疗反应的见解。

IF 3.5 4区生物学

FEBS Letters Pub Date : 2025-05-19 DOI: 10.1002/1873-3468.70071

Marta Sampietro, Marco Cellani, Cristina Scielzo

{"title":"B cell mechanobiology in health and disease: emerging techniques and insights into therapeutic responses.","authors":"Marta Sampietro, Marco Cellani, Cristina Scielzo","doi":"10.1002/1873-3468.70071","DOIUrl":"https://doi.org/10.1002/1873-3468.70071","url":null,"abstract":"Cells sense physical cues from their environment and convert them into biochemical responses through mechanotransduction. Unlike solid tumours, the role of such forces in haematological cancers is underexplored. In this context, immune cells experience dynamic mechanical stimuli as they migrate, extravasate and home to specific tissues. Understanding how these forces shape B-cell function and malignancy represents a groundbreaking area of research. This review examines the key mechanosensory pathways and molecules involved in lymphocyte mechanotransduction, beginning with mechanosensory proteins at the plasma membrane, followed by intracellular signal propagation through the cytoskeleton, eventually highlighting the nucleus as a 'signal actuator'. Subsequently, we cover some measurement approaches and advanced systems to investigate tumour biomechanics, highlighting their application in the context of B cells. Finally, we focus on the implications of mechanobiology in leukaemia, identifying molecules involved in B-cell malignancies that could serve as potential 'mechano-targets' for personalised therapies. This review emphasises the need to understand how lymphocytes generate, sense and respond to mechanical stimuli, which could open avenues for future biomedical innovations. Impact statement Our review is particularly valuable in highlighting the underexplored role of mechanobiology in B cell function and malignancies, while also discussing emerging techniques that can advance this research area. It bridges mechanotransduction, immunology, and cancer biology in a way that will be of interest to researchers across these three main fields.","PeriodicalId":12142,"journal":{"name":"FEBS Letters","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144093006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biophysical characterization of a putative antimicrobial peptide-binding protein of Escherichia coli highlights its dual functionality. 一种推定的抗菌肽结合蛋白的生物物理特性突出了其双重功能。

IF 3.5 4区生物学

FEBS Letters Pub Date : 2025-05-19 DOI: 10.1002/1873-3468.70068

Pratik Dasgupta, Shankar Prasad Kanaujia

{"title":"Biophysical characterization of a putative antimicrobial peptide-binding protein of Escherichia coli highlights its dual functionality.","authors":"Pratik Dasgupta, Shankar Prasad Kanaujia","doi":"10.1002/1873-3468.70068","DOIUrl":"https://doi.org/10.1002/1873-3468.70068","url":null,"abstract":"Antimicrobial peptides (AMPs) disrupt the integrity of the bacterial membrane, ultimately leading to their death. In counter-defense, pathogens are reported to have developed systems such as the sensitivity to antimicrobial peptides (Sap) transport system that evade the action of AMPs and sequester essential micronutrients. However, recent contrasting reports cloud the functional prospects of the Sap system. Hence, this study aimed to characterize the Escherichia coli Sap (EcSap) transport system using biophysical techniques. The results obtained from various approaches suggested the binding of heme to the substrate-binding component (EcSapA) of the EcSap system. Further, this study suggests the interaction of EcSapA with the AMP protamine. In summary, the findings of this study suggest the dual ligand-binding ability of EcSapA. Impact statement The present study reports the functional prospects of the enigmatic substrate-binding protein SapA of E. coli. This analysis highlights the essentiality of the intra-protein disulfide bonds in maintaining the structural integrity of EcSapA. Further, biophysical studies of EcSapA highlight its dual ligand binding propensity, earmarking it as a drug target.","PeriodicalId":12142,"journal":{"name":"FEBS Letters","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144093028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0