FEBS Letters最新文献

Mapping the evolution of mitochondrial complex I through structural variation. 通过结构变异绘制线粒体复合体I的进化图谱。

IF 3 4区生物学

FEBS Letters Pub Date : 2025-10-10 DOI: 10.1002/1873-3468.70181

Dong-Woo Shin, Tingting Chen, James A Letts

{"title":"Mapping the evolution of mitochondrial complex I through structural variation.","authors":"Dong-Woo Shin, Tingting Chen, James A Letts","doi":"10.1002/1873-3468.70181","DOIUrl":"https://doi.org/10.1002/1873-3468.70181","url":null,"abstract":"Respiratory complex I (CI) is a multi-subunit membrane protein complex important for the production of ATP via the oxidative phosphorylation pathway. The structure of CI is roughly conserved across species and is composed of subunits that are either embedded in the membrane or are exposed to the aqueous environment that together form an overall L-shaped 'boot'. The conserved core of CI is generally composed of 14 subunits. Across species, various less conserved 'supernumerary' or 'accessory' subunits have been added. Accessory subunits vary in number across species and can include proteins that are unique to specific lineages. Additionally, there are structural variations in the core subunits between clades. In this Review, we compare seven representative CI structures from divergent eukaryotic lineages to identify what aspects of the CI core subunits are susceptible to variation and classify eukaryotic accessory subunits into those conserved from the last eukaryotic common ancestor (LECA) or those that are lineage specific. Impact statement Understanding the biodiversity and evolution of mitochondrial complex I will reveal patterns that may reflect metabolic niche and can be used to constrain quantitative models of molecular evolution.","PeriodicalId":12142,"journal":{"name":"FEBS Letters","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145274221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Marfan Europe Network: Together we can! 马凡欧洲网：携手共进！

IF 3 4区生物学

FEBS Letters Pub Date : 2025-10-10 DOI: 10.1002/1873-3468.70183

Margit Aschenbrenner, Tuija Ekegren, Françoise Steinbach, Julia Hansen

引用次数: 0

We need more women in STEM. 我们需要更多的女性进入STEM领域。

IF 3 4区生物学

FEBS Letters Pub Date : 2025-10-09 DOI: 10.1002/1873-3468.70178

Laura Norton

引用次数: 0

RAD50 missense variants differentially affect the DNA damage response and mitotic progression. RAD50错义变体对DNA损伤反应和有丝分裂进程的影响不同。

IF 3 4区生物学

FEBS Letters Pub Date : 2025-10-01 DOI: 10.1002/1873-3468.70175

Hanna Redeker, Swantje Kebel, Lea Völkening, Anna Vatselia, Louisa Weinhold, Girmay Asgedom, Axel Schambach, Detlev Schindler, Thilo Dörk, Kristine Bousset

{"title":"RAD50 missense variants differentially affect the DNA damage response and mitotic progression.","authors":"Hanna Redeker, Swantje Kebel, Lea Völkening, Anna Vatselia, Louisa Weinhold, Girmay Asgedom, Axel Schambach, Detlev Schindler, Thilo Dörk, Kristine Bousset","doi":"10.1002/1873-3468.70175","DOIUrl":"https://doi.org/10.1002/1873-3468.70175","url":null,"abstract":"RAD50 is the central protein of the MRN complex and crucial in DNA double-strand break repair. RAD50 deficiency causes a genomic instability disorder characterized by microcephaly and stunted growth. Using lentiviral constructs, we investigated whether cancer-related RAD50 missense variants can complement the delayed damage response after exposure to the chemotherapeutic agent epirubicin and/or mitotic progression in RAD50-deficient fibroblasts. Eight missense variants, all capable of forming an MRN complex, supported the DNA damage response and mitotic features to different extents, indicating these functions are separable. Three variants showed both an impaired epirubicin response and slowed cell division in the likely pathogenic range. Assessing RAD50 missense variants with distinct functional readouts may help to further elucidate their differential roles in immunodeficiency and cancer and could improve therapeutic strategies. Impact statement RAD50 has a strong impact on DNA repair and cancer therapy. Here, we analyse RAD50 missense variants at four functional levels. Some variants showed an impaired epirubicin response and mitotic progression in the pathological range, while for others these endpoints were separable. Functional heterogeneity of RAD50 variants could contribute to clinical variability.","PeriodicalId":12142,"journal":{"name":"FEBS Letters","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145198668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Serial amplification of tau filaments using Alzheimer's brain homogenates and C322A or C322S recombinant tau. 使用阿尔茨海默病脑匀浆和C322A或C322S重组tau蛋白对tau蛋白丝进行序列扩增。

IF 3 4区生物学

FEBS Letters Pub Date : 2025-09-30 DOI: 10.1002/1873-3468.70141

Alessia Santambrogio, Sofia Lövestam, Michael A Metrick, Thomas Löhr, Peifeng Xu, Nicholas C T Gallagher, Bernardino Ghetti, Byron Caughey, Sjors H W Scheres, Michele Vendruscolo

{"title":"Serial amplification of tau filaments using Alzheimer's brain homogenates and C322A or C322S recombinant tau.","authors":"Alessia Santambrogio, Sofia Lövestam, Michael A Metrick, Thomas Löhr, Peifeng Xu, Nicholas C T Gallagher, Bernardino Ghetti, Byron Caughey, Sjors H W Scheres, Michele Vendruscolo","doi":"10.1002/1873-3468.70141","DOIUrl":"https://doi.org/10.1002/1873-3468.70141","url":null,"abstract":"The assembly of tau into amyloid filaments is a hallmark of Alzheimer's disease (AD) and other tauopathies. Cryo-EM revealed the existence of disease-specific tau folds, which are challenging to replicate in vitro. We studied three full-length recombinant 0N3R tau forms (the wild-type and the C322A and C322S variants) using an RT-QuIC assay with brain homogenate seeding. C322A tau formed filaments resembling AD paired helical filaments (PHFs) but with a more open C-shaped core. C322S tau yielded structurally distinct filaments with an ordered C-terminal region. Both mutants seeded further aggregation, whereas the wild-type showed poor reproducibility and mainly unfolded aggregates. These results highlight the importance of optimised conditions to produce disease-relevant tau filaments and aid the development of targeted therapies. Impact statement We investigated the seeded assembly of 0N3R tau and its two mutational variants C322A and C322S, using Alzheimer's disease brain homogenates in a real-time quaking-induced conversion (RT-QuIC) assay. The C322A variant formed filaments partially resembling the AD PHF structure, showing the importance of optimised conditions to produce disease-relevant tau filaments.","PeriodicalId":12142,"journal":{"name":"FEBS Letters","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145198851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hsp104-Hsp70-Hsp110 chaperones disintegrate kinase aggregates formed upon stress in Saccharomyces cerevisiae. 在酿酒酵母中，Hsp104-Hsp70-Hsp110伴侣蛋白可分解在胁迫下形成的激酶聚集体。

IF 3 4区生物学

FEBS Letters Pub Date : 2025-09-29 DOI: 10.1002/1873-3468.70174

Pramit Bhattacharjee, Atin K Mandal

引用次数: 0

Revealing the structure of land plant photosystem II: the journey from negative-stain EM to cryo-EM. 揭示陆地植物光系统II的结构：从负染色电镜到低温电镜的历程。

IF 3 4区生物学

FEBS Letters Pub Date : 2025-09-25 DOI: 10.1002/1873-3468.70173

Roman Kouřil

{"title":"Revealing the structure of land plant photosystem II: the journey from negative-stain EM to cryo-EM.","authors":"Roman Kouřil","doi":"10.1002/1873-3468.70173","DOIUrl":"https://doi.org/10.1002/1873-3468.70173","url":null,"abstract":"Photosystem II (PSII) is a multi-subunit pigment-protein complex in plant chloroplasts that drives water oxidation and oxygen evolution in photosynthesis. In land plants, PSII forms dimeric core complexes associated with multiple light-harvesting complex II (LHCII) antenna proteins (LHCB1-6), assembling into large PSII-LHCII supercomplexes within thylakoid membranes. Over the past few decades, our understanding of PSII structure has advanced dramatically, evolving from low-resolution electron microscopy (EM) data to near-atomic models. This review highlights key milestones in PSII structural research, focusing on how progressive advances in EM from early negative-stain imaging to high-resolution single-particle cryo-EM, have revealed the organization of PSII supercomplexes in various plant species, ranging from angiosperms (e.g., Arabidopsis thaliana, spinach, pea) to gymnosperms (e.g., Norway spruce). These developments have led to the most detailed structural models of plant PSII to date. Future work will aim not only to determine PSII structures from a broader range of organisms, but also to explore the organization and dynamics of larger assemblies such as PSII megacomplexes and PSII-partner complexes, both in isolation and in situ within the thylakoid membrane.","PeriodicalId":12142,"journal":{"name":"FEBS Letters","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145137018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Protein kinase FAM20C-when subcellular localization matters. 蛋白激酶fam20c -当亚细胞定位重要时。

IF 3 4区生物学

FEBS Letters Pub Date : 2025-09-19 DOI: 10.1002/1873-3468.70172

Francesca Noventa, Mauro Salvi

引用次数: 0

TOR signaling on membranes. 细胞膜上的TOR信号。

IF 3 4区生物学

FEBS Letters Pub Date : 2025-09-19 DOI: 10.1002/1873-3468.70171

Robbie Loewith, Lucas Tafur

引用次数: 0

RETRACTION: Evidence That Low Doses of Taxol Enhance the Functional Transactivatory Properties of p53 on p21 Waf Promoter in MCF-7 Breast Cancer Cells. 撤回：低剂量紫杉醇增强MCF-7乳腺癌细胞中p53对p21 Waf启动子的功能转激活特性的证据。

IF 3 4区生物学

FEBS Letters Pub Date : 2025-09-18 DOI: 10.1002/1873-3468.70168

{"title":"RETRACTION: Evidence That Low Doses of Taxol Enhance the Functional Transactivatory Properties of p53 on p21 Waf Promoter in MCF-7 Breast Cancer Cells.","authors":"","doi":"10.1002/1873-3468.70168","DOIUrl":"https://doi.org/10.1002/1873-3468.70168","url":null,"abstract":"Retraction: M. L. Panno , F. Giordano , F. Mastroianni , C. Morelli , E. Brunelli , M. G. Palma , M. Pellegrino , S. Aquila , A. Miglietta , L. Mauro , D. Bonofiglio , and S. Andò , \"Evidence That Low Doses of Taxol Enhance the Functional Transactivatory Properties of p53 on p21 Waf Promoter in MCF-7 Breast Cancer Cells,\" FEBS Letters 580, no. 9 (2006): 2371-2380, https://doi.org/10.1016/j.febslet.2006.03.055. The above article, published online on 29 March 2006 in Wiley Online Library (wileyonlinelibrary.com), has been retracted by agreement between the journal Editor-in-Chief, Michael Brunner; FEBS Press; and John Wiley and Sons Ltd. The retraction has been agreed upon following an investigation prompted by concerns raised by a third party. The investigation identified inappropriate duplications of image sections in Figures 4, 5, and 10. The authors' explanation was found to be insufficient to resolve these concerns, and due to the time elapsed since the original publication, the raw data could no longer be retrieved. However, the extent and nature of the inconsistencies identified in the published figures raised significant concerns regarding the data. As a result, the editors no longer have confidence in the integrity of the presented data and consider the study's conclusions to be compromised. The authors do not agree with this retraction.","PeriodicalId":12142,"journal":{"name":"FEBS Letters","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145080125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0