对早期斑马鱼发育过程中mRNA 3‘末端的综合分析揭示了全基因组范围内3’ utr变化的动态。

IF 3.5 4区生物学 Q1 Biochemistry, Genetics and Molecular Biology

FEBS Letters Pub Date : 2025-07-01 DOI:10.1002/1873-3468.70106

Ludivine Fierro, Anna Ishii, Haruka Aoyama, Toshihiro Arae, Yukako Chiba, Tomoya Kotani

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引用次数: 0

摘要

卵子积累了超过10000个mrna，从这些mrna合成蛋白质或以暂时控制的方式合成蛋白质。尽管这些mrna翻译状态的变化对早期发育至关重要，但胚胎如何协调它们仍不清楚。在这里，我们利用斑马鱼胚胎的3‘端rna测序和计算方法研究了mRNA 3’非翻译区（UTRs）的变化。与我们之前的发现一致，pou5f3 mRNA在发育早期的3'UTR处缩短，数千种mRNA显示3'UTR缩短。此外，我们发现胚胎中的大多数mrna含有几个不同的3'端，并且它们的比例是动态变化的。这些变化与蛋白质合成相结合。我们的研究结果揭示了全基因组3'端动态调控生物过程。

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Comprehensive analysis of mRNA 3' ends during early zebrafish development reveals dynamics of 3'UTR changes on a genome-wide scale.

Eggs accumulate more than 10 000 mRNAs, from which proteins are synthesized constitutively or in a temporally controlled manner. Although changes in the translation state of these mRNAs are crucial for early development, how embryos orchestrate them remains unclear. Here, we investigated changes in mRNA 3' untranslated regions (UTRs) using 3' end-RNA sequencing of zebrafish embryos and computational methods. Consistent with our previous finding that pou5f3 mRNA is shortened at the 3'UTR during early development, thousands of mRNAs showed shortening of the 3'UTRs. Moreover, we found that most mRNAs in embryos contained several different 3' ends and their proportion was dynamically changed. These changes were coupled with protein synthesis. Our results reveal genome-wide 3'-end dynamics in the regulation of biological processes.

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来源期刊

FEBS Letters 生物-生化与分子生物学

CiteScore

7.00

自引率

2.90%

发文量

303

审稿时长

1.0 months

期刊介绍： FEBS Letters is one of the world''s leading journals in molecular biology and is renowned both for its quality of content and speed of production. Bringing together the most important developments in the molecular biosciences, FEBS Letters provides an international forum for Minireviews, Research Letters and Hypotheses that merit urgent publication.