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G protein-coupled oestrogen receptor regulates branched-chain amino acid metabolism through c-Jun N-terminal kinase
IF 3.5 4区 生物学
FEBS Letters Pub Date : 2025-03-06 DOI: 10.1002/1873-3468.70030
Anshu Gupta, Prasad Govind Shinde, Sachin Jorvekar, Akash Suresh Humane, Mythri Chandrasekaran, Roshan M. Borkar, Sudhagar Selvaraju
{"title":"G protein-coupled oestrogen receptor regulates branched-chain amino acid metabolism through c-Jun N-terminal kinase","authors":"Anshu Gupta,&nbsp;Prasad Govind Shinde,&nbsp;Sachin Jorvekar,&nbsp;Akash Suresh Humane,&nbsp;Mythri Chandrasekaran,&nbsp;Roshan M. Borkar,&nbsp;Sudhagar Selvaraju","doi":"10.1002/1873-3468.70030","DOIUrl":"10.1002/1873-3468.70030","url":null,"abstract":"<p>Branched-chain amino acids (BCAA) are essential requirements for overall protein turnover, signalling and energy balance, and dysregulation of their metabolic pathway has been associated with many pathophysiological events. Despite the importance of BCAA in human health, our understanding of their metabolic regulation is limited. Here, we present evidence that G protein-coupled oestrogen receptor (GPER) activation inhibits the key BCAA metabolic regulatory enzyme branched-chain α-keto acid dehydrogenase complex (BCKDH) by phosphorylating S293. Inhibition of BCKDH results in leucine, isoleucine and valine accumulation in cells. Interestingly, GPER did not alter the levels of the kinase BCKDK and the phosphatase PPM1K, which regulate BCKDH activity, but activated MAPK signalling. Using gene silencing, we identified that JNK intercedes GPER-mediated BCKDH inhibition. Together, our results demonstrate that GPER inhibits BCAA metabolism through JNK signalling.</p>","PeriodicalId":12142,"journal":{"name":"FEBS Letters","volume":"599 6","pages":"892-900"},"PeriodicalIF":3.5,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143566409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Changes in importin levels promote nuclear proteasomal degradation of cell cycle-related proteins during THP-1 monocyte-to-macrophage differentiation
IF 3.5 4区 生物学
FEBS Letters Pub Date : 2025-03-05 DOI: 10.1002/1873-3468.70020
Makoto Kimura, Yutaka Ogawa, Shoko Motohashi, Naoko Imamoto
{"title":"Changes in importin levels promote nuclear proteasomal degradation of cell cycle-related proteins during THP-1 monocyte-to-macrophage differentiation","authors":"Makoto Kimura,&nbsp;Yutaka Ogawa,&nbsp;Shoko Motohashi,&nbsp;Naoko Imamoto","doi":"10.1002/1873-3468.70020","DOIUrl":"10.1002/1873-3468.70020","url":null,"abstract":"<p>Importin family nucleocytoplasmic transport receptors share thousands of cargo proteins. To elucidate cell regulatory mechanisms via transport regulation, we analyzed the levels of transport receptors by western blotting and quantified the total cellular and nuclear proteins during monocyte-to-macrophage differentiation of THP-1 cells using mass spectrometry. Importin-α1 decreased and importin-α5 increased during the differentiation. Cell cycle-related proteins decreased in both whole cells and nuclei, and proteasome-related proteins increased in the nuclei but not in whole cells. During the differentiation with importin-α1 overexpression, the nuclear levels of some cell division-related proteins recovered, and with importin-α5 knockdown, proteasome assembly factors decreased in the nuclei. In this differentiation, transport receptors reduce unnecessary nuclear proteins by abating import and promoting nuclear proteasomal degradation. This study demonstrates the importance of global nuclear transport control in cell regulation.</p>","PeriodicalId":12142,"journal":{"name":"FEBS Letters","volume":"599 6","pages":"813-827"},"PeriodicalIF":3.5,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143556401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Making tau amyloid models in vitro: a crucial and underestimated challenge.
IF 3.5 4区 生物学
FEBS Letters Pub Date : 2025-03-03 DOI: 10.1002/1873-3468.70022
Julien Broc, Clara Piersson, Yann Fichou
{"title":"Making tau amyloid models in vitro: a crucial and underestimated challenge.","authors":"Julien Broc, Clara Piersson, Yann Fichou","doi":"10.1002/1873-3468.70022","DOIUrl":"https://doi.org/10.1002/1873-3468.70022","url":null,"abstract":"<p><p>The protein Tau accumulates in the brain as insoluble deposits in a number of neurodegenerative diseases called tauopathies. In vitro work studying the properties of reconstituted tau assemblies has played a major role in understanding the fundamental mechanisms underlying tauopathies. Yet, in our view, the extent to which tau in vitro models represent the assemblies found in the brain is not sufficiently addressed. Here, we provide an overview of the procedures used to form tau amyloids in vitro and we discuss their similarities and differences with brain deposits. We emphasize that, to date, further work is needed to establish how to form accurate models of tau deposits with recombinant protein, let alone how to represent disease-specific properties that discriminate tauopathies.</p>","PeriodicalId":12142,"journal":{"name":"FEBS Letters","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143540839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The intracellular domain of TLR2 is capable of high-affinity Zn binding: possible outcomes for the receptor activation.
IF 3.5 4区 生物学
FEBS Letters Pub Date : 2025-03-03 DOI: 10.1002/1873-3468.70026
Vladislav A Lushpa, Cong Lin, Irina A Talyzina, Marina V Goncharuk, Eduard V Bocharov, Alexander S Arseniev, Xiaohui Wang, Sergey A Goncharuk, Konstantin S Mineev
{"title":"The intracellular domain of TLR2 is capable of high-affinity Zn binding: possible outcomes for the receptor activation.","authors":"Vladislav A Lushpa, Cong Lin, Irina A Talyzina, Marina V Goncharuk, Eduard V Bocharov, Alexander S Arseniev, Xiaohui Wang, Sergey A Goncharuk, Konstantin S Mineev","doi":"10.1002/1873-3468.70026","DOIUrl":"https://doi.org/10.1002/1873-3468.70026","url":null,"abstract":"<p><p>Toll-like receptors (TLRs) are important players in the innate immune system. Binding of pathogen-related molecules to the extracellular domains of TLRs initiates signalosome assembly, a key event in signal transduction. Despite extensive research on individual receptor domains, the mechanism of signalosome assembly remains unclear. Recent evidence suggests that the intracellular TIR domain of TLR1 binds zinc ions, with cysteines playing a pivotal role in binding and receptor activation. This study explores the zinc-binding ability of the TLR2 TIR domain (TLR2<sub>TIR</sub>). We found that TLR2<sub>TIR</sub> binds zinc with nanomolar affinity through its cysteine residues. Two of them, C673 and C713, are essential for receptor activation. These results suggest that zinc may be involved in the initiation of signalosome assembly.</p>","PeriodicalId":12142,"journal":{"name":"FEBS Letters","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143540790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Taurine promotes glucagon-like peptide-1 secretion in enteroendocrine L cells.
IF 3.5 4区 生物学
FEBS Letters Pub Date : 2025-03-03 DOI: 10.1002/1873-3468.70014
Yuri Osuga, Kazuki Harada, Takuya Yamauchi, Tetsuya Kitaguchi, Masami Yokota Hirai, Mitsuharu Matsumoto, Takashi Tsuboi
{"title":"Taurine promotes glucagon-like peptide-1 secretion in enteroendocrine L cells.","authors":"Yuri Osuga, Kazuki Harada, Takuya Yamauchi, Tetsuya Kitaguchi, Masami Yokota Hirai, Mitsuharu Matsumoto, Takashi Tsuboi","doi":"10.1002/1873-3468.70014","DOIUrl":"https://doi.org/10.1002/1873-3468.70014","url":null,"abstract":"<p><p>Taurine, an amino-sulfonic acid mainly sourced from food, suppresses blood glucose by stimulating insulin secretion from pancreatic β-cells. However, its relationship with glucagon-like peptide-1 (GLP-1) secretion from enteroendocrine L cells is unclear. This study aimed to determine the role of taurine in GLP-1 secretion from L cells. Taurine administration promoted GLP-1 secretion in enteroendocrine L cell line GLUTag cells and increased plasma GLP-1 in mice. Taurine uptake via the taurine transporter increased cytosolic ATP levels, resulting in higher intracellular Ca<sup>2+</sup> concentrations and enhanced GLP-1 secretion through ATP-sensitive K<sup>+</sup> channel closure. These findings may help identify new therapeutic targets for obesity and diet-related disease prevention.</p>","PeriodicalId":12142,"journal":{"name":"FEBS Letters","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143540789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
PDRG1 is essential for early plant development as a component of the prefoldin-like complex.
IF 3.5 4区 生物学
FEBS Letters Pub Date : 2025-03-03 DOI: 10.1002/1873-3468.70024
Laura Hernández-Villa, Alberto Palacios-Abella, Yaiza Gómez-Mínguez, Cecilia Costigliolo-Rojas, Eugenio G Minguet, David Alabadí
{"title":"PDRG1 is essential for early plant development as a component of the prefoldin-like complex.","authors":"Laura Hernández-Villa, Alberto Palacios-Abella, Yaiza Gómez-Mínguez, Cecilia Costigliolo-Rojas, Eugenio G Minguet, David Alabadí","doi":"10.1002/1873-3468.70024","DOIUrl":"https://doi.org/10.1002/1873-3468.70024","url":null,"abstract":"<p><p>p53 AND DNA DAMAGE-REGULATED GENE1 (PDRG1) is part of the prefoldin-like complex (PFDLc) in plants and animals. Whether PDRG1 acts primarily as a subunit of PFDLc or as an independent subunit is not known in any eukaryote. Here, we show that impairment of PDRG1 activity in Arabidopsis thaliana leads to embryonic lethality, as is the case for the other prefoldin-like proteins UXT and AtURI. The subunits of PFDLc are the main interactors of PDRG1 in vivo, and the interactomes of PDRG1, UXT, and AtURI show strong overlaps, including subunits of nuclear RNA polymerases and various complexes of the spliceosome. Our results show that PDRG1 plays an essential role in Arabidopsis mainly as a subunit of PFDLc.</p>","PeriodicalId":12142,"journal":{"name":"FEBS Letters","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143540785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The multiple roles of the NlpC_P60 peptidase family in mycobacteria - an underexplored target for antimicrobial drug discovery.
IF 3.5 4区 生物学
FEBS Letters Pub Date : 2025-03-03 DOI: 10.1002/1873-3468.70021
Catharina Dos Santos Silva, Marcio Vinicius Bertacine Dias
{"title":"The multiple roles of the NlpC_P60 peptidase family in mycobacteria - an underexplored target for antimicrobial drug discovery.","authors":"Catharina Dos Santos Silva, Marcio Vinicius Bertacine Dias","doi":"10.1002/1873-3468.70021","DOIUrl":"https://doi.org/10.1002/1873-3468.70021","url":null,"abstract":"<p><p>The main function of the cell wall is to maintain cellular integrity throughout the cell cycle by keeping the cell shape during growth and division. However, far from being a static structure, the cell wall undergoes constant recycling and even molecular modifications of its components. The major component of the bacterial cell wall is the peptidoglycan layer. The balance between peptidoglycan synthesis and degradation is crucial for cell viability and proliferation. Hence, factors involved in the control of peptidoglycan turnover are considered interesting targets for drug development. Members of the NlpC_P60 superfamily of peptidases have been described to participate in the physiology and pathogenesis of several bacterial lineages. However, the knowledge about NlpC_P60-like proteins from mycobacteria is still limited, despite the great progress in recent years. In this Review, we aimed to compile the information about mycobacterial NlpC_P60 proteins, pointing out their distribution across pathogenic and environmental Mycobacterium species, highlighting the knowledge gaps and describing their structural features, role in the physiology and mycobacterial pathogenesis.</p>","PeriodicalId":12142,"journal":{"name":"FEBS Letters","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143540791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Cell proliferation and cell death during whole-body regeneration in the demosponge Halisarca dujardinii.
IF 3.5 4区 生物学
FEBS Letters Pub Date : 2025-02-27 DOI: 10.1002/1873-3468.70025
Andrey I Lavrov, Nikolai P Melnikov, Fyodor V Bolshakov, Daniyal M Saidov, Emilie Le Goff, Kseniia V Skorentseva, Veronika S Frolova, Alexander V Ereskovsky
{"title":"Cell proliferation and cell death during whole-body regeneration in the demosponge Halisarca dujardinii.","authors":"Andrey I Lavrov, Nikolai P Melnikov, Fyodor V Bolshakov, Daniyal M Saidov, Emilie Le Goff, Kseniia V Skorentseva, Veronika S Frolova, Alexander V Ereskovsky","doi":"10.1002/1873-3468.70025","DOIUrl":"https://doi.org/10.1002/1873-3468.70025","url":null,"abstract":"<p><p>Sponges (phylum Porifera) are early-branching metazoans demonstrating an outstanding example of whole-body regeneration, cell reaggregation. During the process, single cells form aggregates capable of progressive development and reconstruction of intact sponges. This study provides the first comprehensive analysis of cell proliferation and cell death during cell reaggregation in a marine demosponge, Halisarca dujardinii. Relatively high proliferative activity occurs at the early and late stages of the reaggregation. This proliferation seems to be a consequence of intact tissue turnover rather than a prerequisite for aggregate development. The contribution of cell death is limited to a short period during the aggregate epithelization. Cell reaggregation in demosponges appears to be a morphallactic restorative process relying primarily on cell migration, dedifferentiation, and transdifferentiation.</p>","PeriodicalId":12142,"journal":{"name":"FEBS Letters","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143515196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
RETRACTION: Ultraviolet B Radiation-Induced Apoptosis in Human Keratinocytes: Cytosolic Activation of Procaspase-8 and the Role of Bcl-2
IF 3.5 4区 生物学
FEBS Letters Pub Date : 2025-02-26 DOI: 10.1002/1873-3468.70019
{"title":"RETRACTION: Ultraviolet B Radiation-Induced Apoptosis in Human Keratinocytes: Cytosolic Activation of Procaspase-8 and the Role of Bcl-2","authors":"","doi":"10.1002/1873-3468.70019","DOIUrl":"10.1002/1873-3468.70019","url":null,"abstract":"<p><b>RETRACTION</b>: Z. Assefa, M. Garmyn, A. Vantieghem, W. Declercq, P. Vandenabeele, J. R. Vandenheede and P. Agostinis, “Ultraviolet B Radiation-Induced Apoptosis in Human Keratinocytes: Cytosolic Activation of Procaspase-8 and the Role of Bcl-2,” <i>FEBS Letters</i> 540, no. 1-3 (2003): 125-132, https://doi.org/10.1016/S0014-5793(03)00238-2.</p><p>The above article, published online on 15 March 2003 in Wiley Online Library (wileyonlinelibrary.com), has been retracted by agreement between; the journal Editor-in-Chief, Michael Brunner; FEBS Press; and John Wiley and Sons Ltd. The retraction has been agreed due to concerns regarding the manipulation of the HaCaT/Bcl-2 blot shown in Figure 1B (in the PDF version of this article). Due to the time that has elapsed since publication, the authors were unable to provide their raw data. The editors found the authors' explanation unsatisfactory and as a result consider the results and conclusions unreliable. The authors disagree with the retraction.</p>","PeriodicalId":12142,"journal":{"name":"FEBS Letters","volume":"599 5","pages":"787"},"PeriodicalIF":3.5,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/1873-3468.70019","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143515197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Human PHOSPHO1 exhibits phosphatidylcholine- and phosphatidylethanolamine-phospholipase C activities and interacts with diacylglycerol kinase δ.
IF 3.5 4区 生物学
FEBS Letters Pub Date : 2025-02-24 DOI: 10.1002/1873-3468.70018
Chiaki Murakami, Kyoko Atsuta-Tsunoda, Sho Inomata, Takuma Kawai, Yasuhisa Hijikata, Kamila Dilimulati, Hiromichi Sakai, Fumio Sakane
{"title":"Human PHOSPHO1 exhibits phosphatidylcholine- and phosphatidylethanolamine-phospholipase C activities and interacts with diacylglycerol kinase δ.","authors":"Chiaki Murakami, Kyoko Atsuta-Tsunoda, Sho Inomata, Takuma Kawai, Yasuhisa Hijikata, Kamila Dilimulati, Hiromichi Sakai, Fumio Sakane","doi":"10.1002/1873-3468.70018","DOIUrl":"https://doi.org/10.1002/1873-3468.70018","url":null,"abstract":"<p><p>Phosphatidylcholine- and phosphatidylethanolamine-specific phospholipase C (PC-PLC and PE-PLC) activities, which generate diacylglycerol (DG) and are tricyclodecan-9-yl-xanthogenate (D609)-sensitive, have been detected in both the membrane and cytosolic fractions. We have previously demonstrated that sphingomyelin synthase isozymes, which are transmembrane proteins, exhibit PC-/PE-PLC activities. However, mammalian cytosolic PC-PLC and PE-PLC remain unidentified. Here, we demonstrated that phosphatase orphan 1 (PHOSPHO1), a cytosolic protein, exhibits D609-sensitive PC-PLC and PE-PLC activities. Moreover, the overexpression of PHOSPHO1 in HEK293 cells significantly increased the levels of cellular saturated and/or monounsaturated fatty acid-containing DG. Furthermore, DGKδ cosedimented and colocalized with PHOSPHO1. Collectively, these in vitro findings provide, for the first time, a promising candidate for the long-sought cytosolic PC-/PE-PLC, which may act as DG supply enzyme upstream of DGKδ.</p>","PeriodicalId":12142,"journal":{"name":"FEBS Letters","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143491434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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