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PDRG1 is essential for early plant development as a component of the prefoldin-like complex.
IF 3.5 4区 生物学
FEBS Letters Pub Date : 2025-03-03 DOI: 10.1002/1873-3468.70024
Laura Hernández-Villa, Alberto Palacios-Abella, Yaiza Gómez-Mínguez, Cecilia Costigliolo-Rojas, Eugenio G Minguet, David Alabadí
{"title":"PDRG1 is essential for early plant development as a component of the prefoldin-like complex.","authors":"Laura Hernández-Villa, Alberto Palacios-Abella, Yaiza Gómez-Mínguez, Cecilia Costigliolo-Rojas, Eugenio G Minguet, David Alabadí","doi":"10.1002/1873-3468.70024","DOIUrl":"https://doi.org/10.1002/1873-3468.70024","url":null,"abstract":"<p><p>p53 AND DNA DAMAGE-REGULATED GENE1 (PDRG1) is part of the prefoldin-like complex (PFDLc) in plants and animals. Whether PDRG1 acts primarily as a subunit of PFDLc or as an independent subunit is not known in any eukaryote. Here, we show that impairment of PDRG1 activity in Arabidopsis thaliana leads to embryonic lethality, as is the case for the other prefoldin-like proteins UXT and AtURI. The subunits of PFDLc are the main interactors of PDRG1 in vivo, and the interactomes of PDRG1, UXT, and AtURI show strong overlaps, including subunits of nuclear RNA polymerases and various complexes of the spliceosome. Our results show that PDRG1 plays an essential role in Arabidopsis mainly as a subunit of PFDLc.</p>","PeriodicalId":12142,"journal":{"name":"FEBS Letters","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143540785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The multiple roles of the NlpC_P60 peptidase family in mycobacteria - an underexplored target for antimicrobial drug discovery.
IF 3.5 4区 生物学
FEBS Letters Pub Date : 2025-03-03 DOI: 10.1002/1873-3468.70021
Catharina Dos Santos Silva, Marcio Vinicius Bertacine Dias
{"title":"The multiple roles of the NlpC_P60 peptidase family in mycobacteria - an underexplored target for antimicrobial drug discovery.","authors":"Catharina Dos Santos Silva, Marcio Vinicius Bertacine Dias","doi":"10.1002/1873-3468.70021","DOIUrl":"https://doi.org/10.1002/1873-3468.70021","url":null,"abstract":"<p><p>The main function of the cell wall is to maintain cellular integrity throughout the cell cycle by keeping the cell shape during growth and division. However, far from being a static structure, the cell wall undergoes constant recycling and even molecular modifications of its components. The major component of the bacterial cell wall is the peptidoglycan layer. The balance between peptidoglycan synthesis and degradation is crucial for cell viability and proliferation. Hence, factors involved in the control of peptidoglycan turnover are considered interesting targets for drug development. Members of the NlpC_P60 superfamily of peptidases have been described to participate in the physiology and pathogenesis of several bacterial lineages. However, the knowledge about NlpC_P60-like proteins from mycobacteria is still limited, despite the great progress in recent years. In this Review, we aimed to compile the information about mycobacterial NlpC_P60 proteins, pointing out their distribution across pathogenic and environmental Mycobacterium species, highlighting the knowledge gaps and describing their structural features, role in the physiology and mycobacterial pathogenesis.</p>","PeriodicalId":12142,"journal":{"name":"FEBS Letters","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143540791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Cell proliferation and cell death during whole-body regeneration in the demosponge Halisarca dujardinii.
IF 3.5 4区 生物学
FEBS Letters Pub Date : 2025-02-27 DOI: 10.1002/1873-3468.70025
Andrey I Lavrov, Nikolai P Melnikov, Fyodor V Bolshakov, Daniyal M Saidov, Emilie Le Goff, Kseniia V Skorentseva, Veronika S Frolova, Alexander V Ereskovsky
{"title":"Cell proliferation and cell death during whole-body regeneration in the demosponge Halisarca dujardinii.","authors":"Andrey I Lavrov, Nikolai P Melnikov, Fyodor V Bolshakov, Daniyal M Saidov, Emilie Le Goff, Kseniia V Skorentseva, Veronika S Frolova, Alexander V Ereskovsky","doi":"10.1002/1873-3468.70025","DOIUrl":"https://doi.org/10.1002/1873-3468.70025","url":null,"abstract":"<p><p>Sponges (phylum Porifera) are early-branching metazoans demonstrating an outstanding example of whole-body regeneration, cell reaggregation. During the process, single cells form aggregates capable of progressive development and reconstruction of intact sponges. This study provides the first comprehensive analysis of cell proliferation and cell death during cell reaggregation in a marine demosponge, Halisarca dujardinii. Relatively high proliferative activity occurs at the early and late stages of the reaggregation. This proliferation seems to be a consequence of intact tissue turnover rather than a prerequisite for aggregate development. The contribution of cell death is limited to a short period during the aggregate epithelization. Cell reaggregation in demosponges appears to be a morphallactic restorative process relying primarily on cell migration, dedifferentiation, and transdifferentiation.</p>","PeriodicalId":12142,"journal":{"name":"FEBS Letters","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143515196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
RETRACTION: Ultraviolet B Radiation-Induced Apoptosis in Human Keratinocytes: Cytosolic Activation of Procaspase-8 and the Role of Bcl-2
IF 3.5 4区 生物学
FEBS Letters Pub Date : 2025-02-26 DOI: 10.1002/1873-3468.70019
{"title":"RETRACTION: Ultraviolet B Radiation-Induced Apoptosis in Human Keratinocytes: Cytosolic Activation of Procaspase-8 and the Role of Bcl-2","authors":"","doi":"10.1002/1873-3468.70019","DOIUrl":"10.1002/1873-3468.70019","url":null,"abstract":"<p><b>RETRACTION</b>: Z. Assefa, M. Garmyn, A. Vantieghem, W. Declercq, P. Vandenabeele, J. R. Vandenheede and P. Agostinis, “Ultraviolet B Radiation-Induced Apoptosis in Human Keratinocytes: Cytosolic Activation of Procaspase-8 and the Role of Bcl-2,” <i>FEBS Letters</i> 540, no. 1-3 (2003): 125-132, https://doi.org/10.1016/S0014-5793(03)00238-2.</p><p>The above article, published online on 15 March 2003 in Wiley Online Library (wileyonlinelibrary.com), has been retracted by agreement between; the journal Editor-in-Chief, Michael Brunner; FEBS Press; and John Wiley and Sons Ltd. The retraction has been agreed due to concerns regarding the manipulation of the HaCaT/Bcl-2 blot shown in Figure 1B (in the PDF version of this article). Due to the time that has elapsed since publication, the authors were unable to provide their raw data. The editors found the authors' explanation unsatisfactory and as a result consider the results and conclusions unreliable. The authors disagree with the retraction.</p>","PeriodicalId":12142,"journal":{"name":"FEBS Letters","volume":"599 5","pages":"787"},"PeriodicalIF":3.5,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/1873-3468.70019","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143515197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Human PHOSPHO1 exhibits phosphatidylcholine- and phosphatidylethanolamine-phospholipase C activities and interacts with diacylglycerol kinase δ.
IF 3.5 4区 生物学
FEBS Letters Pub Date : 2025-02-24 DOI: 10.1002/1873-3468.70018
Chiaki Murakami, Kyoko Atsuta-Tsunoda, Sho Inomata, Takuma Kawai, Yasuhisa Hijikata, Kamila Dilimulati, Hiromichi Sakai, Fumio Sakane
{"title":"Human PHOSPHO1 exhibits phosphatidylcholine- and phosphatidylethanolamine-phospholipase C activities and interacts with diacylglycerol kinase δ.","authors":"Chiaki Murakami, Kyoko Atsuta-Tsunoda, Sho Inomata, Takuma Kawai, Yasuhisa Hijikata, Kamila Dilimulati, Hiromichi Sakai, Fumio Sakane","doi":"10.1002/1873-3468.70018","DOIUrl":"https://doi.org/10.1002/1873-3468.70018","url":null,"abstract":"<p><p>Phosphatidylcholine- and phosphatidylethanolamine-specific phospholipase C (PC-PLC and PE-PLC) activities, which generate diacylglycerol (DG) and are tricyclodecan-9-yl-xanthogenate (D609)-sensitive, have been detected in both the membrane and cytosolic fractions. We have previously demonstrated that sphingomyelin synthase isozymes, which are transmembrane proteins, exhibit PC-/PE-PLC activities. However, mammalian cytosolic PC-PLC and PE-PLC remain unidentified. Here, we demonstrated that phosphatase orphan 1 (PHOSPHO1), a cytosolic protein, exhibits D609-sensitive PC-PLC and PE-PLC activities. Moreover, the overexpression of PHOSPHO1 in HEK293 cells significantly increased the levels of cellular saturated and/or monounsaturated fatty acid-containing DG. Furthermore, DGKδ cosedimented and colocalized with PHOSPHO1. Collectively, these in vitro findings provide, for the first time, a promising candidate for the long-sought cytosolic PC-/PE-PLC, which may act as DG supply enzyme upstream of DGKδ.</p>","PeriodicalId":12142,"journal":{"name":"FEBS Letters","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143491434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Unraveling Mycobacterium tuberculosis acid resistance and pH homeostasis mechanisms.
IF 3.5 4区 生物学
FEBS Letters Pub Date : 2025-02-24 DOI: 10.1002/1873-3468.70023
Janïs Laudouze, Stéphane Canaan, Alexandre Gouzy, Pierre Santucci
{"title":"Unraveling Mycobacterium tuberculosis acid resistance and pH homeostasis mechanisms.","authors":"Janïs Laudouze, Stéphane Canaan, Alexandre Gouzy, Pierre Santucci","doi":"10.1002/1873-3468.70023","DOIUrl":"https://doi.org/10.1002/1873-3468.70023","url":null,"abstract":"<p><p>Mycobacterium tuberculosis (Mtb) is a successful pathogen that has developed a variety of strategies to survive and replicate within host cells. Among them, resistance mechanisms that allow Mtb to cope with the acidic environment of the endolysosomal network play a crucial role in its host adaptation and pathogenesis. In this Review, we summarize our understanding regarding the molecular factors contributing to Mtb acid resistance and the maintenance of intrabacterial pH homeostasis within in vitro and in cellulo systems. We also discuss some of the recently emerged concepts regarding host adaptation to acid stress, with a special emphasis on central carbon metabolism. Finally, we introduce some of the current and future direction(s) in this fast-evolving field of research.</p>","PeriodicalId":12142,"journal":{"name":"FEBS Letters","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143491435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Acute oxygen sensing by arterial chemoreceptors with a mutant mitochondrial complex I ND6 subunit lacking reverse electron transport.
IF 3.5 4区 生物学
FEBS Letters Pub Date : 2025-02-21 DOI: 10.1002/1873-3468.70017
María Torres-López, Pedro F Spiller, Lin Gao, Paula García-Flores, Michael P Murphy, Patricia Ortega-Sáenz, José López-Barneo
{"title":"Acute oxygen sensing by arterial chemoreceptors with a mutant mitochondrial complex I ND6 subunit lacking reverse electron transport.","authors":"María Torres-López, Pedro F Spiller, Lin Gao, Paula García-Flores, Michael P Murphy, Patricia Ortega-Sáenz, José López-Barneo","doi":"10.1002/1873-3468.70017","DOIUrl":"https://doi.org/10.1002/1873-3468.70017","url":null,"abstract":"<p><p>Carotid body glomus cells are essential for stimulating breathing in response to hypoxia. They contain specialized mitochondria in which hypoxia induces the accumulation of NADH and H<sub>2</sub>O<sub>2</sub> that modulate membrane ion channel activity. We investigated whether hypoxia induces reverse electron transport (RET) at mitochondrial complex I (MCI). We studied glomus cells from mice with a mutation in ND6, a core protein of MCI, which maintain normal MCI NADH dehydrogenase activity but cannot catalyze RET. The ND6 mutation increases the propensity of MCI to deactivate, and glomus cells with deactivated MCI are insensitive to acute hypoxia. These findings further indicate that MCI function is necessary for glomus cell responsiveness to hypoxia, although MCI RET does not seem to be required for this process.</p>","PeriodicalId":12142,"journal":{"name":"FEBS Letters","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143467451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Structural differences in the outer membrane-associated flagellar rings between sheathed and unsheathed flagella.
IF 3.5 4区 生物学
FEBS Letters Pub Date : 2025-02-20 DOI: 10.1002/1873-3468.70011
Przemysław Dutka, Ethan H Li, Tengfei Zhong, Grant J Jensen, Mohammed Kaplan
{"title":"Structural differences in the outer membrane-associated flagellar rings between sheathed and unsheathed flagella.","authors":"Przemysław Dutka, Ethan H Li, Tengfei Zhong, Grant J Jensen, Mohammed Kaplan","doi":"10.1002/1873-3468.70011","DOIUrl":"https://doi.org/10.1002/1873-3468.70011","url":null,"abstract":"<p><p>The bacterial flagellar motor generates a torque to move the bacterium in its environment. Despite sharing a conserved core, flagellar motors of different species exhibit structural diversity with species-specific embellishments. These embellishments are classified into various types, including integrated (spanning the whole periplasmic space) or outer membrane (OM)-associated ones. Here, we used cryo-electron tomography to investigate the structural differences between the embellishments of sheathed and unsheathed flagella in various species. We discovered that the integrated embellishments of sheathed flagella have disks and rings with a constant diameter, while those of unsheathed flagella have components that vary significantly in diameter. Both unsheathed and sheathed flagella with OM-associated embellishments have components with constant diameter with a subset of motors having an additional extracellular ring. In this Hypothesis article, we propose that these differences may play a role in the formation of the sheath, as having large protein disks of various diameters underneath the OM may interfere with membrane bending to form the sheath.</p>","PeriodicalId":12142,"journal":{"name":"FEBS Letters","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143457410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Structural insights into the Shigella flexneri GmvAT toxin-antitoxin system.
IF 3.5 4区 生物学
FEBS Letters Pub Date : 2025-02-20 DOI: 10.1002/1873-3468.70015
Ran Chen, Hui Zhao, Jie Zhou, Aoyun Liu, Yinfeng Guo, Kejue Wu, Yongle Xiang, Jinping Lei, Songshan Jiang, Wei Xie
{"title":"Structural insights into the Shigella flexneri GmvAT toxin-antitoxin system.","authors":"Ran Chen, Hui Zhao, Jie Zhou, Aoyun Liu, Yinfeng Guo, Kejue Wu, Yongle Xiang, Jinping Lei, Songshan Jiang, Wei Xie","doi":"10.1002/1873-3468.70015","DOIUrl":"https://doi.org/10.1002/1873-3468.70015","url":null,"abstract":"<p><p>Toxin-antitoxin (TA) systems are common bicistronic gene elements in bacteria and are critical for stress responses. The toxin members of the GNAT/RHH TA family can acetylate certain aminoacylated tRNA molecules and inhibit global protein translation. One member named GmvT is important for virulence plasmid maintenance in Shigella flexneri, but the underlying mechanism remains poorly understood. Here, we report the cocrystal structures of GmvT in two forms. The binding of the antitoxin mainly relies on the backbone of the toxin while the cofactor is free of contacts with the antitoxin, supported by follow-up in vitro and in vivo studies. Our study provides insight into the protein-protein/protein-ligand interactions of the GmvAT pair and the structural basis for molecular recognition.</p>","PeriodicalId":12142,"journal":{"name":"FEBS Letters","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143457411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Clathrin functions in intracellular sorting during receptor-mediated endocytosis.
IF 3.5 4区 生物学
FEBS Letters Pub Date : 2025-02-20 DOI: 10.1002/1873-3468.70016
Mai Itagaki, Noriyasu Kamei
{"title":"Clathrin functions in intracellular sorting during receptor-mediated endocytosis.","authors":"Mai Itagaki, Noriyasu Kamei","doi":"10.1002/1873-3468.70016","DOIUrl":"https://doi.org/10.1002/1873-3468.70016","url":null,"abstract":"<p><p>Clathrin is a key protein involved in receptor-mediated endocytosis (RME), which is also known as clathrin-mediated endocytosis (CME). Ligand/receptor binding facilitates clathrin clustering and cell surface invagination. We recently found that the typical CME ligand transferrin (Tf) can be internalized into cells without the clathrin heavy chain (CLTC). In this study, we clarified the unknown roles of clathrin during intracellular trafficking. Microscopic observation revealed that Tf accumulates in the deeper compartments of CLTC-knockdown HeLa cells. A colocalization analysis with endosome/lysosome markers showed that endocytosed Tf moves to early/late endosomes and then to lysosomes in CLTC-knockdown cells. This suggests that clathrin contributes to the recycling and exocytosis of endocytosed molecules.</p>","PeriodicalId":12142,"journal":{"name":"FEBS Letters","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143457403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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