FEBS Letters最新文献_第2页

A guide for blood-brain barrier models. 血脑屏障模型指南

IF 3.5 4区生物学

FEBS Letters Pub Date : 2024-11-12 DOI: 10.1002/1873-3468.15053

Yomna Soliman, Jana Al-Khodor, Gülnaz Yildirim Köken, Nur Mustafaoglu

{"title":"A guide for blood-brain barrier models.","authors":"Yomna Soliman, Jana Al-Khodor, Gülnaz Yildirim Köken, Nur Mustafaoglu","doi":"10.1002/1873-3468.15053","DOIUrl":"https://doi.org/10.1002/1873-3468.15053","url":null,"abstract":"Understanding the intricate mechanisms underlying brain-related diseases hinges on unraveling the pivotal role of the blood-brain barrier (BBB), an essential dynamic interface crucial for maintaining brain equilibrium. This review offers a comprehensive analysis of BBB physiology, delving into its cellular and molecular components while exploring a wide range of in vivo and in vitro BBB models. Notably, recent advancements in 3D cell culture techniques are explicitly discussed, as they have significantly improved the fidelity of BBB modeling by enabling the replication of physiologically relevant environments under flow conditions. Special attention is given to the cellular aspects of in vitro BBB models, alongside discussions on advances in stem cell technologies, providing valuable insights into generating robust cellular systems for BBB modeling. The diverse array of cell types used in BBB modeling, depending on their sources, is meticulously examined in this comprehensive review, scrutinizing their respective derivation protocols and implications. By synthesizing diverse approaches, this review sheds light on the improvements of BBB models to capture physiological conditions, aiding in understanding BBB interactions in health and disease conditions to foster clinical developments.","PeriodicalId":12142,"journal":{"name":"FEBS Letters","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142617479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Glu592 of the axon guidance receptor ROBO3 mediates a pH-dependent interaction with NELL2 ligand. 轴突导向受体 ROBO3 的 Glu592 介导了与 NELL2 配体的 pH 依赖性相互作用。

IF 3.5 4区生物学

FEBS Letters Pub Date : 2024-11-12 DOI: 10.1002/1873-3468.15054

Kimihiko Mizutani, Mayuko Toyoda, Teruyo Ojima-Kato, Andrés D Maturana, Tomoaki Niimi

引用次数: 0

FoxO1 signaling in B cell malignancies and its therapeutic targeting. B 细胞恶性肿瘤中的 FoxO1 信号转导及其治疗靶点。

IF 3.5 4区生物学

FEBS Letters Pub Date : 2024-11-12 DOI: 10.1002/1873-3468.15057

Krystof Hlavac, Petra Pavelkova, Laura Ondrisova, Marek Mraz

{"title":"FoxO1 signaling in B cell malignancies and its therapeutic targeting.","authors":"Krystof Hlavac, Petra Pavelkova, Laura Ondrisova, Marek Mraz","doi":"10.1002/1873-3468.15057","DOIUrl":"https://doi.org/10.1002/1873-3468.15057","url":null,"abstract":"FoxO transcription factors (FoxO1, FoxO3a, FoxO4, FoxO6) are a highly evolutionary conserved subfamily of the 'forkhead' box proteins. They have traditionally been considered tumor suppressors, but FoxO1 also exhibits oncogenic properties. The complex nature of FoxO1 is illustrated by its various roles in B cell development and differentiation, immunoglobulin gene rearrangement and cell-surface B cell receptor (BCR) structure, DNA damage control, cell cycle regulation, and germinal center reaction. FoxO1 is tightly regulated at a transcriptional (STAT3, HEB, EBF, FoxOs) and post-transcriptional level (Akt, AMPK, CDK2, GSK3, IKKs, JNK, MAPK/Erk, SGK1, miRNA). In B cell malignancies, recurrent FoxO1 activating mutations (S22/T24) and aberrant nuclear export and activity have been described, underscoring the potential of its therapeutic inhibition. Here, we review FoxO1's roles across B cell and myeloid malignancies, namely acute lymphoblastic leukemia (ALL), acute myeloid leukemia (AML), chronic lymphocytic leukemia (CLL), follicular lymphoma (FL), diffuse large B cell lymphoma (DLBCL), mantle cell lymphoma (MCL), Burkitt lymphoma (BL), Hodgkin lymphoma (HL), and multiple myeloma (MM). We also discuss preclinical evidence for FoxO1 targeting by currently available inhibitors (AS1708727, AS1842856, cpd10).","PeriodicalId":12142,"journal":{"name":"FEBS Letters","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142617499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cordycepin generally inhibits growth factor signal transduction in a systems pharmacology study. 在一项系统药理学研究中，虫草素通常会抑制生长因子信号转导。

IF 3.5 4区生物学

FEBS Letters Pub Date : 2024-11-07 DOI: 10.1002/1873-3468.15046

Steven Lawrence, Jialiang Lin, Asma Khurshid, Wahyu Utami, Richa Singhania, Sadaf Ashraf, Graeme J Thorn, Irengbam Rocky Mangangcha, Keith Spriggs, Dong-Hyun Kim, David Barrett, Cornelia H de Moor

引用次数: 0

14-3-3ε conditional knockout mice exhibit defects in the development of the epidermis. 14-3-3ε 条件性基因敲除小鼠表现出表皮发育缺陷。

IF 3.5 4区生物学

FEBS Letters Pub Date : 2024-11-07 DOI: 10.1002/1873-3468.15051

Sarika Tilwani, Karan Gandhi, Sorab N Dalal

引用次数: 0

Cryo-EM structure of a novel α-synuclein filament subtype from multiple system atrophy. 多系统萎缩症中一种新型α-突触核蛋白丝亚型的冷冻电子显微镜结构。

IF 3.5 4区生物学

FEBS Letters Pub Date : 2024-11-07 DOI: 10.1002/1873-3468.15048

Nicholas L Yan, Francisco Candido, Eric Tse, Arthur A Melo, Stanley B Prusiner, Daniel A Mordes, Daniel R Southworth, Nick A Paras, Gregory E Merz

引用次数: 0

Cryo-EM structures of the zinc transporters ZnT3 and ZnT4 provide insights into their transport mechanisms. 锌转运体 ZnT3 和 ZnT4 的低温电子显微镜（Cryo-EM）结构有助于深入了解它们的转运机制。

IF 3.5 4区生物学

FEBS Letters Pub Date : 2024-10-30 DOI: 10.1002/1873-3468.15047

Hanako Ishida, Riri Yo, Zhikuan Zhang, Toshiyuki Shimizu, Umeharu Ohto

引用次数: 0

Leishmania donovani adenylosuccinate synthetase requires IMP for dimerization and organization of the active site. 唐氏利什曼原虫腺苷琥珀酸合成酶的二聚化和活性位点的组织需要 IMP。

IF 3.5 4区生物学

FEBS Letters Pub Date : 2024-10-27 DOI: 10.1002/1873-3468.15040

Jigneshkumar A Mochi, Jaykumar Jani, Smit Shah, Anju Pappachan

引用次数: 0

Odz4 upregulates SAN-specific genes to promote differentiation into cardiac pacemaker-like cells. Odz4上调SAN特异性基因，促进心脏起搏器样细胞的分化。

IF 3.5 4区生物学

FEBS Letters Pub Date : 2024-10-27 DOI: 10.1002/1873-3468.15036

Anqi Dong, Masao Yoshizumi, Hiroki Kokubo

{"title":"Odz4 upregulates SAN-specific genes to promote differentiation into cardiac pacemaker-like cells.","authors":"Anqi Dong, Masao Yoshizumi, Hiroki Kokubo","doi":"10.1002/1873-3468.15036","DOIUrl":"https://doi.org/10.1002/1873-3468.15036","url":null,"abstract":"Cardiac arrhythmias stemming from abnormal sinoatrial node (SAN) function can lead to sudden death. Developing a biological pacemaker device for treating sick sinus syndrome (SSS) could offer a potential cure. Understanding SAN differentiation is crucial, yet its regulatory mechanism remains unclear. We reanalyzed published RNA-seq data and identified Odz4 as a SAN-specific candidate. In situ hybridization revealed Odz4 expression in the cardiac crescent and throughout the cardiac conduction system (CCS). To assess the role of Odz4 in CCS differentiation, we utilized a Tet-Off inducible system for its intracellular domain (ICD). Embryonic bodies (EBs) exogenously expressing Odz4-ICD exhibited an increased propensity to develop into pacemaker-like cells with enhanced automaticity and upregulated expression of SAN-specific genes. CellChat and GO analyses unveiled SAN-specific enrichment of ligand-receptor sets, especially Ptn-Ncl, and extracellular matrix components in the group exogenously expressing Odz4-ICD. Our findings underscore the significance of Odz4 in SAN development and offer fresh insights into biological pacemaker establishment.","PeriodicalId":12142,"journal":{"name":"FEBS Letters","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142497669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Crystal structures of Aspergillus oryzae exo-β-(1,3)-glucanase reveal insights into oligosaccharide binding, recognition, and hydrolysis. 黑曲霉外β-(1,3)-葡聚糖酶的晶体结构揭示了寡糖的结合、识别和水解过程。

IF 3.5 4区生物学

FEBS Letters Pub Date : 2024-10-24 DOI: 10.1002/1873-3468.15045

Barnava Banerjee, Chinmay K Kamale, Abhishek B Suryawanshi, Subrata Dasgupta, Santosh Noronha, Prasenjit Bhaumik

{"title":"Crystal structures of Aspergillus oryzae exo-β-(1,3)-glucanase reveal insights into oligosaccharide binding, recognition, and hydrolysis.","authors":"Barnava Banerjee, Chinmay K Kamale, Abhishek B Suryawanshi, Subrata Dasgupta, Santosh Noronha, Prasenjit Bhaumik","doi":"10.1002/1873-3468.15045","DOIUrl":"https://doi.org/10.1002/1873-3468.15045","url":null,"abstract":"Exo-β-(1,3)-glucanases are promising enzymes for use in the biofuel industry as they hydrolyse sugars such as laminarin, a major constituent of the algal cell wall. This study reports structural and biochemical characterizations of Aspergillus oryzae exo-β-(1,3)-glucanase (AoBgl) belonging to the GH5 family. Purified AoBgl hydrolyses β-(1,3)-glycosidic linkages of the oligosaccharide laminaritriose and the polysaccharide laminarin effectively. We have determined three high-resolution structures of AoBgl: (a) the apo form at 1.75 Å, (b) the complexed form with bound cellobiose at 1.73 Å and (c) the glucose-bound form at 1.20 Å. The crystal structures, molecular dynamics simulation studies and site-directed mutagenesis reveal the mode of substrate binding and interactions at the active site. The results also indicate that AoBgl effectively hydrolyses trisaccharides and higher oligosaccharides. The findings from our structural and biochemical studies would aid in rational engineering efforts to generate superior AoBgl variants and similar GH5 enzymes for their industrial use.","PeriodicalId":12142,"journal":{"name":"FEBS Letters","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142497592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0