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Spontaneous and chaperone-assisted metal loading in the active site of protein phosphatase-1 蛋白磷酸酶-1 活性位点的自发金属负载和伴侣辅助金属负载。
IF 3.5 4区 生物学
FEBS Letters Pub Date : 2024-09-08 DOI: 10.1002/1873-3468.15012
Gerd Van der Hoeven, Sarah Lemaire, Xinyu Cao, Zander Claes, Spyridoula Karamanou, Mathieu Bollen
{"title":"Spontaneous and chaperone-assisted metal loading in the active site of protein phosphatase-1","authors":"Gerd Van der Hoeven,&nbsp;Sarah Lemaire,&nbsp;Xinyu Cao,&nbsp;Zander Claes,&nbsp;Spyridoula Karamanou,&nbsp;Mathieu Bollen","doi":"10.1002/1873-3468.15012","DOIUrl":"10.1002/1873-3468.15012","url":null,"abstract":"<p>Protein phosphatase PP1 has two active-site metals (Zn<sup>2+</sup>/Fe<sup>2+</sup>) that are essential for catalysis. However, when expressed in bacteria, PP1 has two Mn<sup>2+</sup>-ions in its active site, indicating that the incorporation of Zn<sup>2+</sup>/Fe<sup>2+</sup> depends on additional eukaryotic component(s). Here, we used purified, metal-deficient PP1 to study metal incorporation. Fe<sup>2+</sup> was incorporated spontaneously, but Zn<sup>2+</sup> was not. Mn<sup>2+</sup>-incorporation at physiological pH depended on the co-expression of PP1 with PPP1R2 (Inhibitor-2) or PPP1R11 (Inhibitor-3), or a pre-incubation of PP1 at pH 4. We also demonstrate that PPP1R2 and PPP1R11 are Zn<sup>2+</sup>-binding proteins but are, by themselves, not able to load PP1 with Zn<sup>2+</sup>. Our data suggest that PPP1R2 and PPP1R11 function as metal chaperones for PP1 but depend on co-chaperone(s) and/or specific modification(s) for the transfer of associated Zn<sup>2+</sup> to PP1.</p>","PeriodicalId":12142,"journal":{"name":"FEBS Letters","volume":"598 23","pages":"2876-2885"},"PeriodicalIF":3.5,"publicationDate":"2024-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11626998/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142153519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Mitochondrial permeability transition mediated by MTCH2 and F-ATP synthase contributes to ferroptosis defense. 由 MTCH2 和 F-ATP 合成酶介导的线粒体通透性转换有助于铁中毒防御。
IF 3.5 4区 生物学
FEBS Letters Pub Date : 2024-09-03 DOI: 10.1002/1873-3468.15008
Lishu Guo
{"title":"Mitochondrial permeability transition mediated by MTCH2 and F-ATP synthase contributes to ferroptosis defense.","authors":"Lishu Guo","doi":"10.1002/1873-3468.15008","DOIUrl":"https://doi.org/10.1002/1873-3468.15008","url":null,"abstract":"<p><p>The opening of the mitochondrial permeability transition pore (PTP), a Ca<sup>2+</sup>-dependent pore located in the inner mitochondrial membrane, triggers mitochondrial outer membrane permeabilization (MOMP) and induces organelle rupture. However, the underlying mechanism of PTP-induced MOMP remains unclear. Mitochondrial carrier homolog 2 (MTCH2) mediates MOMP process by facilitating the recruitment of tBID to mitochondria. Here, we show that MTCH2 binds to cyclophilin D (CyPD) and promotes the dimerization of F-ATP synthase via interaction with subunit j. The interplay between MTCH2 and subunit j coordinates MOMP and PTP to mediate the occurrence of mitochondrial permeability transition. Knockdown of CyPD, MTCH2 and subunit j markedly sensitizes cells to RSL3-induced ferroptosis, which is prevented by MitoTEMPO, suggesting that mitochondrial permeability transition mediates ferroptosis defense.</p>","PeriodicalId":12142,"journal":{"name":"FEBS Letters","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142125225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A shift in chromatin binding of phosphorylated p38 precedes transcriptional changes upon oxidative stress 在氧化应激发生转录变化之前，磷酸化 p38 的染色质结合发生了变化。
IF 3.5 4区 生物学
FEBS Letters Pub Date : 2024-09-01 DOI: 10.1002/1873-3468.15006
Carlos Camilleri-Robles, Paula Climent-Cantó, Palmira Llorens-Giralt, Cecilia C. Klein, Florenci Serras, Montserrat Corominas
{"title":"A shift in chromatin binding of phosphorylated p38 precedes transcriptional changes upon oxidative stress","authors":"Carlos Camilleri-Robles,&nbsp;Paula Climent-Cantó,&nbsp;Palmira Llorens-Giralt,&nbsp;Cecilia C. Klein,&nbsp;Florenci Serras,&nbsp;Montserrat Corominas","doi":"10.1002/1873-3468.15006","DOIUrl":"10.1002/1873-3468.15006","url":null,"abstract":"<p>P38 mitogen-activated protein kinases are key in the regulation of the cellular response to stressors. P38 is known to regulate transcription, mRNA processing, stability, and translation. The transcriptional changes mediated by phosphorylated p38 (P-p38) in response to extracellular stimuli have been thoroughly analyzed in many tissues and organisms. However, the genomic localization of chromatin-associated P-p38 remains poorly understood. Here, we analyze the chromatin binding of activated P-p38 and its role in the response to reactive oxygen species (ROS) in <i>Drosophila</i> S2 cells. We found that P-p38 is already bound to chromatin in basal conditions. After ROS exposure, chromatin-associated P-p38 relocates towards genes involved in the recovery process. Our findings highlight the role of P-p38 dynamic chromatin binding in orchestrating gene expression responses to oxidative stress.</p>","PeriodicalId":12142,"journal":{"name":"FEBS Letters","volume":"598 23","pages":"2926-2938"},"PeriodicalIF":3.5,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11627000/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142105843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Structural modeling and characterization of the Mycobacterium tuberculosis MmpL3 C-terminal domain 结核分枝杆菌 MmpL3 C 端结构域的结构建模和特征描述。
IF 3.5 4区 生物学
FEBS Letters Pub Date : 2024-08-28 DOI: 10.1002/1873-3468.15007
Naomi Berkowitz, Allison MacMillan, Marit B. Simmons, Ujwal Shinde, Georgiana E. Purdy
{"title":"Structural modeling and characterization of the Mycobacterium tuberculosis MmpL3 C-terminal domain","authors":"Naomi Berkowitz,&nbsp;Allison MacMillan,&nbsp;Marit B. Simmons,&nbsp;Ujwal Shinde,&nbsp;Georgiana E. Purdy","doi":"10.1002/1873-3468.15007","DOIUrl":"10.1002/1873-3468.15007","url":null,"abstract":"<p>The <i>Mycobacterium tuberculosis</i> (<i>Mtb</i>) cell envelope provides a protective barrier against the immune response and antibiotics. The mycobacterial membrane protein large (MmpL) family of proteins export cell envelope lipids and siderophores; therefore, these proteins are important for the basic biology and pathogenicity of <i>Mtb</i>. In particular, MmpL3 is essential and a known drug target. Despite interest in MmpL3, the structural data in the field are incomplete. Utilizing homology modeling, AlphaFold, and biophysical techniques, we characterized the cytoplasmic C-terminal domain (CTD) of MmpL3 to better understand its structure and function. Our <i>in silico</i> models of the MmpL11<sub>TB</sub> and MmpL3<sub>TB</sub> CTD reveal notable features including a long unstructured linker that connects the globular domain to the last transmembrane (TM) in each transporter, charged lysine and arginine residues facing the membrane, and a C-terminal alpha helix. Our predicted overall structure enables a better understanding of these transporters.</p>","PeriodicalId":12142,"journal":{"name":"FEBS Letters","volume":"598 21","pages":"2734-2747"},"PeriodicalIF":3.5,"publicationDate":"2024-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142092566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Structural basis of sugar recognition by SCFFBS2 ubiquitin ligase involved in NGLY1 deficiency 参与 NGLY1 缺乏症的 SCFFBS2 泛素连接酶识别糖的结构基础
IF 3.5 4区 生物学
FEBS Letters Pub Date : 2024-08-22 DOI: 10.1002/1873-3468.15003
Tadashi Satoh, Maho Yagi-Utsumi, Nozomi Ishii, Tsunehiro Mizushima, Hirokazu Yagi, Ryuichi Kato, Yuriko Tachida, Hiroaki Tateno, Ichiro Matsuo, Koichi Kato, Tadashi Suzuki, Yukiko Yoshida
{"title":"Structural basis of sugar recognition by SCFFBS2 ubiquitin ligase involved in NGLY1 deficiency","authors":"Tadashi Satoh,&nbsp;Maho Yagi-Utsumi,&nbsp;Nozomi Ishii,&nbsp;Tsunehiro Mizushima,&nbsp;Hirokazu Yagi,&nbsp;Ryuichi Kato,&nbsp;Yuriko Tachida,&nbsp;Hiroaki Tateno,&nbsp;Ichiro Matsuo,&nbsp;Koichi Kato,&nbsp;Tadashi Suzuki,&nbsp;Yukiko Yoshida","doi":"10.1002/1873-3468.15003","DOIUrl":"10.1002/1873-3468.15003","url":null,"abstract":"<p>The cytosolic peptide:<i>N</i>-glycanase (PNGase) is involved in the quality control of <i>N</i>-glycoproteins via the endoplasmic reticulum-associated degradation (ERAD) pathway. Mutations in the gene encoding cytosolic PNGase (<i>NGLY1</i> in humans) cause NGLY1 deficiency. Recent findings indicate that the F-box protein FBS2 of the SCF<sup>FBS2</sup> ubiquitin ligase complex can be a promising drug target for NGLY1 deficiency. Here, we determined the crystal structure of bovine FBS2 complexed with the adaptor protein SKP1 and a sugar ligand, Man<sub>3</sub>GlcNAc<sub>2</sub>, which corresponds to the core pentasaccharide of <i>N</i>-glycan. Our crystallographic data together with NMR data revealed the structural basis of disparate sugar-binding specificities in homologous FBS proteins and identified a potential druggable pocket for <i>in silico</i> docking studies. Our results provide a potential basis for the development of selective inhibitors against FBS2 in NGLY1 deficiency.</p>","PeriodicalId":12142,"journal":{"name":"FEBS Letters","volume":"598 18","pages":"2259-2268"},"PeriodicalIF":3.5,"publicationDate":"2024-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142016821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Metabolic dysregulation-triggered neutrophil extracellular traps exacerbate acute liver failure 代谢失调触发的中性粒细胞胞外捕获物会加剧急性肝衰竭。
IF 3.5 4区 生物学
FEBS Letters Pub Date : 2024-08-18 DOI: 10.1002/1873-3468.14971
Kangnan Zhang, Rongrong Jia, Qinghui Zhang, Shihao Xiang, Na Wang, Ling Xu
{"title":"Metabolic dysregulation-triggered neutrophil extracellular traps exacerbate acute liver failure","authors":"Kangnan Zhang,&nbsp;Rongrong Jia,&nbsp;Qinghui Zhang,&nbsp;Shihao Xiang,&nbsp;Na Wang,&nbsp;Ling Xu","doi":"10.1002/1873-3468.14971","DOIUrl":"10.1002/1873-3468.14971","url":null,"abstract":"<p>Acute liver failure (ALF) is an acute liver disease with a high mortality rate in clinical practice, characterized histologically by extensive hepatocellular necrosis and massive neutrophil infiltration. However, the role of these abnormally infiltrating neutrophils during ALF development is unclear. Here, in an ALF mouse model, metabolites were identified that promote the formation of neutrophil extracellular traps (NETs) in the liver, subsequently influencing macrophage differentiation and disease progression. ALF occurs with abnormalities in hepatic and intestinal metabolites. Abnormal metabolites (LTD4 and glutathione) can directly, or indirectly <i>via</i> reactive oxygen species, promote NET formation of infiltrating neutrophils, which subsequently regulate macrophages in a pro-inflammatory M1-like state, inducing an amplification of the destructive effects of inflammation. Together, this study provides new insights into the role of NETs in the pathogenesis of ALF.</p>","PeriodicalId":12142,"journal":{"name":"FEBS Letters","volume":"598 19","pages":"2450-2462"},"PeriodicalIF":3.5,"publicationDate":"2024-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141999646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The miR-26a/SIRT6/HIF-1α axis regulates glycolysis and inflammatory responses in host macrophages during Mycobacterium tuberculosis infection 在结核分枝杆菌感染期间，miR-26a/SIRT6/HIF-1α轴调节宿主巨噬细胞中的糖酵解和炎症反应。
IF 3.5 4区 生物学
FEBS Letters Pub Date : 2024-08-18 DOI: 10.1002/1873-3468.15001
Soumya Mal, Debayan Majumder, Pankaj Birari, Arun Kumar Sharma, Umesh Gupta, Kuladip Jana, Manikuntala Kundu, Joyoti Basu
{"title":"The miR-26a/SIRT6/HIF-1α axis regulates glycolysis and inflammatory responses in host macrophages during Mycobacterium tuberculosis infection","authors":"Soumya Mal,&nbsp;Debayan Majumder,&nbsp;Pankaj Birari,&nbsp;Arun Kumar Sharma,&nbsp;Umesh Gupta,&nbsp;Kuladip Jana,&nbsp;Manikuntala Kundu,&nbsp;Joyoti Basu","doi":"10.1002/1873-3468.15001","DOIUrl":"10.1002/1873-3468.15001","url":null,"abstract":"<p><i>Mycobacterium tuberculosis</i> (<i>Mtb</i>) is the causative agent of tuberculosis. Here, a macrophage infection model was used to unravel the role of the histone deacetylase sirtuin 6 (SIRT6) in <i>Mtb</i>-triggered regulation of the innate immune response. <i>Mtb</i> infection downregulated microRNA-26a and upregulated its target SIRT6. SIRT6 suppressed glycolysis and expression of HIF-1α-dependent glycolytic genes during infection. In addition, SIRT6 regulated the levels of intracellular succinate which controls stabilization of HIF-1α, as well as the release of interleukin (IL)-1β. Furthermore, SIRT6 inhibited inducible nitric oxide synthase (iNOS) and proinflammatory IL-6 but augmented anti-inflammatory arginase expression. The miR-26a/SIRT6/HIF-1α axis therefore regulates glycolysis and macrophage immune responses during <i>Mtb</i> infection. Our findings link SIRT6 to rewiring of macrophage signaling pathways facilitating dampening of the antibacterial immune response.</p>","PeriodicalId":12142,"journal":{"name":"FEBS Letters","volume":"598 20","pages":"2592-2614"},"PeriodicalIF":3.5,"publicationDate":"2024-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141999659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The structural complexity of pyomelanin impacts UV shielding in Pseudomonas species with different lifestyles 焦褐藻素的结构复杂性影响不同生活方式假单胞菌的紫外线屏蔽能力。
IF 3.5 4区 生物学
FEBS Letters Pub Date : 2024-08-16 DOI: 10.1002/1873-3468.15000
Mateo N. Diaz Appella, Adriana Kolender, Oscar J. Oppezzo, Nancy I. López, Paula M. Tribelli
{"title":"The structural complexity of pyomelanin impacts UV shielding in Pseudomonas species with different lifestyles","authors":"Mateo N. Diaz Appella,&nbsp;Adriana Kolender,&nbsp;Oscar J. Oppezzo,&nbsp;Nancy I. López,&nbsp;Paula M. Tribelli","doi":"10.1002/1873-3468.15000","DOIUrl":"10.1002/1873-3468.15000","url":null,"abstract":"<p>Pyomelanin, a polymeric pigment in <i>Pseudomonas</i>, arises mainly from alterations in tyrosine degradation. The chemical structure of pyomelanin remains elusive due to its heterogeneous nature. Here, we report strain-specific differences in pyomelanin structural features across <i>Pseudomonas</i> using PAO1 and PA14 reference strains carrying mutations in <i>hmgA</i> (a gene involved in pyomelanin synthesis), a melanogenic <i>P. aeruginosa</i> clinical isolate (PAM), and a melanogenic <i>P. extremaustralis</i> (PexM). UV spectra showed dual peaks for PAO1 and PA14 mutants and single peaks for PAM and PexM. FTIR phenol : alcohol ratio changes and complex NMR spectra indicated non-linear polymers. UVC radiation survival increased with pyomelanin addition, correlating with pigment absorption attenuation. <i>P. extremaustralis</i> UVC survival varied with melanin source, with PAO1 pyomelanin being the most protective. These findings delineate structure-based pyomelanin subgroups, having distinct physiological effects.</p>","PeriodicalId":12142,"journal":{"name":"FEBS Letters","volume":"598 21","pages":"2702-2716"},"PeriodicalIF":3.5,"publicationDate":"2024-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141995590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Crystal structure and enzyme engineering of the broad substrate spectrum l-amino acid oxidase 4 from the fungus Hebeloma cylindrosporum 来自真菌圆柱孢 Hebeloma cylindrosporum 的广底物谱 l- 氨基酸氧化酶 4 的晶体结构和酶工程。
IF 3.5 4区 生物学
FEBS Letters Pub Date : 2024-08-16 DOI: 10.1002/1873-3468.15002
Simon Koopmeiners, Dominic Gilzer, Christiane Widmann, Nils Berelsmann, Jens Sproß, Hartmut H. Niemann, Gabriele Fischer von Mollard
{"title":"Crystal structure and enzyme engineering of the broad substrate spectrum l-amino acid oxidase 4 from the fungus Hebeloma cylindrosporum","authors":"Simon Koopmeiners,&nbsp;Dominic Gilzer,&nbsp;Christiane Widmann,&nbsp;Nils Berelsmann,&nbsp;Jens Sproß,&nbsp;Hartmut H. Niemann,&nbsp;Gabriele Fischer von Mollard","doi":"10.1002/1873-3468.15002","DOIUrl":"10.1002/1873-3468.15002","url":null,"abstract":"<p><span>l</span>-Amino acid oxidases (LAAOs) catalyze the oxidative deamination of <span>l</span>-amino acids to α-keto acids. Recombinant production of LAAOs with broad substrate spectrum remains a formidable challenge. We previously achieved this for the highly active and thermostable LAAO4 of <i>Hebeloma cylindrosporum</i> (<i>Hc</i>LAAO4). Here, we crystallized a proteolytically truncated surface entropy reduction variant of <i>Hc</i>LAAO4 and solved its structure in substrate-free form and in complex with diverse substrates. The ability to support the aliphatic portion of a substrate's side chain by an overall hydrophobic active site is responsible for the broad substrate spectrum of <i>Hc</i>LAAO4, including <span>l</span>-amino acids with big aromatic, acidic and basic side chains. Based on the structural findings, we generated an E288H variant with increased activity toward pharmaceutical building blocks of high interest.</p>","PeriodicalId":12142,"journal":{"name":"FEBS Letters","volume":"598 18","pages":"2306-2320"},"PeriodicalIF":3.5,"publicationDate":"2024-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/1873-3468.15002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141995588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
T-cell immunosuppression in sepsis is augmented by sciatic denervation-induced skeletal muscle atrophy 坐骨神经支配引起的骨骼肌萎缩增强了败血症中的 T 细胞免疫抑制。
IF 3.5 4区 生物学
FEBS Letters Pub Date : 2024-08-08 DOI: 10.1002/1873-3468.14999
Sumika Osa, Yuki Enoki, Daisuke Takahashi, Victor Tuan Giam Chuang, Kazuaki Taguchi, Kazuaki Matsumoto
{"title":"T-cell immunosuppression in sepsis is augmented by sciatic denervation-induced skeletal muscle atrophy","authors":"Sumika Osa,&nbsp;Yuki Enoki,&nbsp;Daisuke Takahashi,&nbsp;Victor Tuan Giam Chuang,&nbsp;Kazuaki Taguchi,&nbsp;Kazuaki Matsumoto","doi":"10.1002/1873-3468.14999","DOIUrl":"10.1002/1873-3468.14999","url":null,"abstract":"<p>Skeletal muscle atrophy is a known risk factor for immunosuppressive conditions and for a poor prognosis in sepsis. However, its immunopathology has not been experimentally elucidated. This study investigated the effects of skeletal muscle atrophy on the immunopathology of sepsis. Male C57BL/6J mice were subjected to sciatic denervation (DN) and caecal ligation and puncture (CLP) to induce muscle atrophy or sepsis. The macrophages, myeloid-derived suppressor cells (MDSC), and T-cells in peritoneal and spleen were analysed using flow cytometry. DN-induced muscle atrophy did not affect macrophage and MDSC populations. In contrast, the percentage of cytotoxic T-lymphocyte-associated antigen (CTLA)-4<sup>+</sup> CD4<sup>+</sup> T-cells, programmed death (PD)-1<sup>+</sup> CD8<sup>+</sup> T-cells, regulatory T-cells, and the CTLA-4<sup>+</sup> regulatory T-cells was statistically significantly higher in DN-CLP mice than in sham-CLP mice. Skeletal muscle atrophy before sepsis triggers excessive T cell immunosuppression, which may contribute to the exacerbation of sepsis under skeletal muscle atrophy.</p>","PeriodicalId":12142,"journal":{"name":"FEBS Letters","volume":"598 20","pages":"2581-2591"},"PeriodicalIF":3.5,"publicationDate":"2024-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/1873-3468.14999","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141906308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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