FEBS Letters最新文献_第3页

Odz4 upregulates SAN-specific genes to promote differentiation into cardiac pacemaker-like cells. Odz4上调SAN特异性基因，促进心脏起搏器样细胞的分化。

IF 3.5 4区生物学

FEBS Letters Pub Date : 2024-10-27 DOI: 10.1002/1873-3468.15036

Anqi Dong, Masao Yoshizumi, Hiroki Kokubo

{"title":"Odz4 upregulates SAN-specific genes to promote differentiation into cardiac pacemaker-like cells.","authors":"Anqi Dong, Masao Yoshizumi, Hiroki Kokubo","doi":"10.1002/1873-3468.15036","DOIUrl":"https://doi.org/10.1002/1873-3468.15036","url":null,"abstract":"Cardiac arrhythmias stemming from abnormal sinoatrial node (SAN) function can lead to sudden death. Developing a biological pacemaker device for treating sick sinus syndrome (SSS) could offer a potential cure. Understanding SAN differentiation is crucial, yet its regulatory mechanism remains unclear. We reanalyzed published RNA-seq data and identified Odz4 as a SAN-specific candidate. In situ hybridization revealed Odz4 expression in the cardiac crescent and throughout the cardiac conduction system (CCS). To assess the role of Odz4 in CCS differentiation, we utilized a Tet-Off inducible system for its intracellular domain (ICD). Embryonic bodies (EBs) exogenously expressing Odz4-ICD exhibited an increased propensity to develop into pacemaker-like cells with enhanced automaticity and upregulated expression of SAN-specific genes. CellChat and GO analyses unveiled SAN-specific enrichment of ligand-receptor sets, especially Ptn-Ncl, and extracellular matrix components in the group exogenously expressing Odz4-ICD. Our findings underscore the significance of Odz4 in SAN development and offer fresh insights into biological pacemaker establishment.","PeriodicalId":12142,"journal":{"name":"FEBS Letters","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142497669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Crystal structures of Aspergillus oryzae exo-β-(1,3)-glucanase reveal insights into oligosaccharide binding, recognition, and hydrolysis. 黑曲霉外β-(1,3)-葡聚糖酶的晶体结构揭示了寡糖的结合、识别和水解过程。

IF 3.5 4区生物学

FEBS Letters Pub Date : 2024-10-24 DOI: 10.1002/1873-3468.15045

Barnava Banerjee, Chinmay K Kamale, Abhishek B Suryawanshi, Subrata Dasgupta, Santosh Noronha, Prasenjit Bhaumik

{"title":"Crystal structures of Aspergillus oryzae exo-β-(1,3)-glucanase reveal insights into oligosaccharide binding, recognition, and hydrolysis.","authors":"Barnava Banerjee, Chinmay K Kamale, Abhishek B Suryawanshi, Subrata Dasgupta, Santosh Noronha, Prasenjit Bhaumik","doi":"10.1002/1873-3468.15045","DOIUrl":"https://doi.org/10.1002/1873-3468.15045","url":null,"abstract":"Exo-β-(1,3)-glucanases are promising enzymes for use in the biofuel industry as they hydrolyse sugars such as laminarin, a major constituent of the algal cell wall. This study reports structural and biochemical characterizations of Aspergillus oryzae exo-β-(1,3)-glucanase (AoBgl) belonging to the GH5 family. Purified AoBgl hydrolyses β-(1,3)-glycosidic linkages of the oligosaccharide laminaritriose and the polysaccharide laminarin effectively. We have determined three high-resolution structures of AoBgl: (a) the apo form at 1.75 Å, (b) the complexed form with bound cellobiose at 1.73 Å and (c) the glucose-bound form at 1.20 Å. The crystal structures, molecular dynamics simulation studies and site-directed mutagenesis reveal the mode of substrate binding and interactions at the active site. The results also indicate that AoBgl effectively hydrolyses trisaccharides and higher oligosaccharides. The findings from our structural and biochemical studies would aid in rational engineering efforts to generate superior AoBgl variants and similar GH5 enzymes for their industrial use.","PeriodicalId":12142,"journal":{"name":"FEBS Letters","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142497592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

RETRACTION: Role of the PDK1-PKB-GSK3 Pathway in Regulating Glycogen Synthase and Glucose Uptake in the Heart. 回归：PDK1-PKB-GSK3 通路在调节心脏糖原合成酶和葡萄糖摄取中的作用。

IF 3.5 4区生物学

FEBS Letters Pub Date : 2024-10-24 DOI: 10.1002/1873-3468.15044

引用次数: 0

Substrate recognition by the 4-hydroxytryptamine kinase PsiK in psilocybin biosynthesis. 迷幻药生物合成过程中 4-羟色胺激酶 PsiK 的底物识别。

IF 3.5 4区生物学

FEBS Letters Pub Date : 2024-10-24 DOI: 10.1002/1873-3468.15042

Kai Rogge, Tobias Johannes Wagner, Dirk Hoffmeister, Bernhard Rupp, Sebastiaan Werten

引用次数: 0

Mapping the sclerostin-LRP4 binding interface identifies critical interaction hotspots in loops 1 and 3 of sclerostin. 绘制硬骨蛋白-LRP4 结合界面图，确定硬骨蛋白环 1 和环 3 中的关键相互作用热点。

IF 3.5 4区生物学