分析b细胞恶性肿瘤细胞外囊泡免疫相互作用的生物学和技术复杂性。

IF 3 4区生物学 Q1 Biochemistry, Genetics and Molecular Biology

FEBS Letters Pub Date : 2025-07-04 DOI:10.1002/1873-3468.70102

Daniel Bachurski, Michael Hallek

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引用次数: 0

摘要

细胞外囊泡（EVs）介导肿瘤免疫微环境中的细胞间通讯。然而，由于EV分离和分析的生物学复杂性和技术挑战，它们在b细胞恶性肿瘤中的作用仍然不明确。通过新颖的标记策略在单EV和单细胞水平上推进EV和受体细胞分析对于克服这些障碍至关重要。本文综述了来自不同来源的EV货物和亚群组成的异质性及其在b细胞恶性肿瘤免疫微环境中的免疫相互作用，同时讨论了分析免疫细胞EV及其靶细胞的创新方法。

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Biological and technical complexities in analyzing extracellular vesicle immune interactions in B-cell malignancies.

Extracellular vesicles (EVs) mediate intercellular communication in tumor immune microenvironments. However, their role in B-cell malignancies remains poorly defined, owing to biological complexity and technical challenges in EV isolation and analysis. Advancing EV and recipient cell analysis at the single-EV and single-cell level via novel tagging strategies is essential to overcome these obstacles. This graphical review examines the heterogeneity of EV cargo and subpopulation composition from diverse sources and their immune interactions in the tumor immune microenvironment of B-cell malignancies, while discussing innovative methods for analyzing immune cell EVs and their target cells.

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来源期刊

FEBS Letters 生物-生化与分子生物学

CiteScore

7.00

自引率

2.90%

发文量

303

审稿时长

1.0 months

期刊介绍： FEBS Letters is one of the world''s leading journals in molecular biology and is renowned both for its quality of content and speed of production. Bringing together the most important developments in the molecular biosciences, FEBS Letters provides an international forum for Minireviews, Research Letters and Hypotheses that merit urgent publication.