{"title":"氨基酰基- trna合成酶的机理和动力学分析。","authors":"Igor Zivkovic, Morana Dulic, Ita Gruic-Sovulj","doi":"10.1002/1873-3468.70098","DOIUrl":null,"url":null,"abstract":"<p><p>Accurate protein synthesis is crucial for life. The key players are aminoacyl-tRNA synthetases (AARSs), which read the genetic code by pairing cognate amino acids and tRNAs. AARSs establish high amino acid selectivity by employing physicochemical limits in molecular recognition. However, chemical and structural resemblance between some amino acids prevents their efficient discrimination by AARSs. In these cases, AARSs hydrolyze the formed non-cognate intermediates or aa-tRNAs to ensure selectivity, establishing complex reaction pathways within the synthetic and editing sites. Understanding AARS mechanisms is crucial for understanding their biology. Here, we review kinetic assays for exploring AARS mechanisms. For each assay, we state the most suitable substrates, product(s) recommended to follow, and, importantly, possible caveats that may lead to the kinetic artefact.</p>","PeriodicalId":12142,"journal":{"name":"FEBS Letters","volume":" ","pages":""},"PeriodicalIF":3.0000,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Mechanisms and kinetic assays of aminoacyl-tRNA synthetases.\",\"authors\":\"Igor Zivkovic, Morana Dulic, Ita Gruic-Sovulj\",\"doi\":\"10.1002/1873-3468.70098\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Accurate protein synthesis is crucial for life. The key players are aminoacyl-tRNA synthetases (AARSs), which read the genetic code by pairing cognate amino acids and tRNAs. AARSs establish high amino acid selectivity by employing physicochemical limits in molecular recognition. However, chemical and structural resemblance between some amino acids prevents their efficient discrimination by AARSs. In these cases, AARSs hydrolyze the formed non-cognate intermediates or aa-tRNAs to ensure selectivity, establishing complex reaction pathways within the synthetic and editing sites. Understanding AARS mechanisms is crucial for understanding their biology. Here, we review kinetic assays for exploring AARS mechanisms. For each assay, we state the most suitable substrates, product(s) recommended to follow, and, importantly, possible caveats that may lead to the kinetic artefact.</p>\",\"PeriodicalId\":12142,\"journal\":{\"name\":\"FEBS Letters\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":3.0000,\"publicationDate\":\"2025-07-04\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"FEBS Letters\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1002/1873-3468.70098\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"Biochemistry, Genetics and Molecular Biology\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"FEBS Letters","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1002/1873-3468.70098","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"Biochemistry, Genetics and Molecular Biology","Score":null,"Total":0}
Mechanisms and kinetic assays of aminoacyl-tRNA synthetases.
Accurate protein synthesis is crucial for life. The key players are aminoacyl-tRNA synthetases (AARSs), which read the genetic code by pairing cognate amino acids and tRNAs. AARSs establish high amino acid selectivity by employing physicochemical limits in molecular recognition. However, chemical and structural resemblance between some amino acids prevents their efficient discrimination by AARSs. In these cases, AARSs hydrolyze the formed non-cognate intermediates or aa-tRNAs to ensure selectivity, establishing complex reaction pathways within the synthetic and editing sites. Understanding AARS mechanisms is crucial for understanding their biology. Here, we review kinetic assays for exploring AARS mechanisms. For each assay, we state the most suitable substrates, product(s) recommended to follow, and, importantly, possible caveats that may lead to the kinetic artefact.
期刊介绍:
FEBS Letters is one of the world''s leading journals in molecular biology and is renowned both for its quality of content and speed of production. Bringing together the most important developments in the molecular biosciences, FEBS Letters provides an international forum for Minireviews, Research Letters and Hypotheses that merit urgent publication.