IF 2.4 4区生物学

Fly Pub Date : 2022-12-01 DOI: 10.1080/19336934.2022.2087484

Yohei Nitta, Atsushi Sugie

引用次数: 0

Ancestral dietary change alters the development of Drosophila larvae through MAPK signalling. 祖先的饮食改变通过MAPK信号改变果蝇幼虫的发育。

IF 1.2 4区生物学

Fly Pub Date : 2022-12-01 DOI: 10.1080/19336934.2022.2088032

Samuel G Towarnicki, Neil A Youngson, Susan M Corley, Jus C St John, Richard G Melvin, Nigel Turner, Margaret J Morris, J William O Ballard

{"title":"Ancestral dietary change alters the development of Drosophila larvae through MAPK signalling.","authors":"Samuel G Towarnicki, Neil A Youngson, Susan M Corley, Jus C St John, Richard G Melvin, Nigel Turner, Margaret J Morris, J William O Ballard","doi":"10.1080/19336934.2022.2088032","DOIUrl":"https://doi.org/10.1080/19336934.2022.2088032","url":null,"abstract":"Studies in a broad range of animal species have revealed phenotypes that are caused by ancestral life experiences, including stress and diet. Ancestral dietary macronutrient composition and quantity (over- and under-nutrition) have been shown to alter descendent growth, metabolism and behaviour. Molecules have been identified in gametes that are changed by ancestral diet and are required for transgenerational effects. However, there is less understanding of the developmental pathways altered by inherited molecules during the period between fertilization and adulthood. To investigate this non-genetic inheritance, we exposed great grand-parental and grand-parental generations to defined protein to carbohydrate (P:C) dietary ratios. Descendent developmental timing was consistently faster in the period between the embryonic and pupal stages when ancestors had a higher P:C ratio diet. Transcriptional analysis revealed extensive and long-lasting changes to the MAPK signalling pathway, which controls growth rate through the regulation of ribosomal RNA transcription. Pharmacological inhibition of both MAPK and rRNA pathways recapitulated the ancestral diet-induced developmental changes. This work provides insight into non-genetic inheritance between fertilization and adulthood.","PeriodicalId":12128,"journal":{"name":"Fly","volume":"16 1","pages":"299-311"},"PeriodicalIF":1.2,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9354765/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10528622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

HaloTag-based reporters for sparse labeling and cell tracking. 基于halotag的报告器，用于稀疏标记和细胞跟踪。

IF 1.2 4区生物学

Fly Pub Date : 2022-12-01 DOI: 10.1080/19336934.2022.2142460

Lydie Couturier, Juan Luna, Khalil Mazouni, Claire Mestdagh, Minh-Son Phan, Francis Corson, Francois Schweisguth

{"title":"HaloTag-based reporters for sparse labeling and cell tracking.","authors":"Lydie Couturier, Juan Luna, Khalil Mazouni, Claire Mestdagh, Minh-Son Phan, Francis Corson, Francois Schweisguth","doi":"10.1080/19336934.2022.2142460","DOIUrl":"https://doi.org/10.1080/19336934.2022.2142460","url":null,"abstract":"Multiscale analysis of morphogenesis requires to follow and measure in real-time the in vivo behaviour of large numbers of individual cells over long period of time. Despite recent progress, the large-scale automated tracking of cells in developing embryos and tissues remains a challenge. Here we describe a genetic tool for the random and sparse labelling of individual cells in developing Drosophila tissues. This tool is based on the conditional expression of a nuclear HaloTag protein that can be fluorescently labelled upon the irreversible binding of a cell permeable synthetic ligand. While the slow maturation of genetically encoded fluorescent renders the tracking of individual cells difficult in rapidly dividing tissues, nuclear HaloTag proteins allowed for rapid labelling of individual cells in cultured imaginal discs. To study cell shape changes, we also produced an HaloTag version of the actin-bound protein LifeAct. Since sparse labelling facilitates cell tracking, nuclear HaloTag reporters will be useful for the single-cell analysis of fate dynamics in Drosophila tissues cultured ex vivo.","PeriodicalId":12128,"journal":{"name":"Fly","volume":"16 1","pages":"360-366"},"PeriodicalIF":1.2,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9635558/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10474465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A screen of small molecule and genetic modulators of life span in female Drosophila identifies etomoxir, RH5849 and unanticipated temperature effects. 对雌果蝇寿命的小分子和基因调节剂进行筛选，发现了依托莫西尔、RH5849 和意想不到的温度效应。

IF 2.4 4区生物学

Fly Pub Date : 2022-12-01 DOI: 10.1080/19336934.2022.2149209

Gary N Landis, Sebastian Ko, Oscar Peng, Brett Bognar, Michael Khmelkov, Hans S Bell, John Tower

{"title":"A screen of small molecule and genetic modulators of life span in female Drosophila identifies etomoxir, RH5849 and unanticipated temperature effects.","authors":"Gary N Landis, Sebastian Ko, Oscar Peng, Brett Bognar, Michael Khmelkov, Hans S Bell, John Tower","doi":"10.1080/19336934.2022.2149209","DOIUrl":"10.1080/19336934.2022.2149209","url":null,"abstract":"Mifepristone increases life span in female Drosophila melanogaster, and its molecular target(s) remain unclear. Here small molecule and genetic interventions were tested for ability to mimic mifepristone, or to decrease life span in a way that can be rescued by mifepristone. Etomoxir inhibits lipid metabolism, and significantly increased life span in virgin and mated females, but not males, at 50 µM concentration. Pioglitazone is reported to activate both mammalian PPARγ and its Drosophila homolog Eip75B. Pioglitazone produced minor and inconsistent benefits for female Drosophila life span, and only at the lowest concentrations tested. Ecdysone is a Drosophila steroid hormone reported to regulate responses to mating, and RH5849 is a potent mimic of ecdysone. RH5849 reduced virgin female life span, and this was partly rescued by mifepristone. Mifepristone did not compete with RH5849 for activation of an ecdysone receptor (EcR)-responsive transgenic reporter, indicating that the relevant target for mifepristone is not EcR. The conditional GAL4/GAL80ts system was used in attempt to test the effect of an Eip75B RNAi construct on female life span. However, the 29°C temperature used for induction reduced or eliminated mating-induced midgut hypertrophy, the negative life span effects of mating, and the positive life span effects of mifepristone. Even when applied after mating was complete, a shift to 29°C temperature reduced mating-induced midgut hypertrophy by half, and the life span effects of mating by 4.8-fold. Taken together, these results identify promising small molecules for further analysis, and inform the design of experiments involving the GAL4/GAL80ts system.","PeriodicalId":12128,"journal":{"name":"Fly","volume":"16 1","pages":"397-413"},"PeriodicalIF":2.4,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9683069/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10474700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The role of micro RNAs (miRNAs) in the regulation of Drosophila melanogaster's innate immunity. 微rna (miRNAs)在果蝇先天免疫调节中的作用。

IF 1.2 4区生物学

Fly Pub Date : 2022-12-01 DOI: 10.1080/19336934.2022.2149204

Max Yang Lu, Stanislava Chtarbanova

引用次数: 2

Interorgan communication through peripherally derived peptide hormones in Drosophila. 果蝇通过外周肽类激素进行器官间交流

IF 2.4 4区生物学

Fly Pub Date : 2022-12-01 DOI: 10.1080/19336934.2022.2061834

Naoki Okamoto, Akira Watanabe

引用次数: 0

Dynamic movement and turnover of extracellular matrices during tissue development and maintenance. 细胞外基质在组织发育和维持过程中的动态运动和周转。

IF 2.4 4区生物学

Fly Pub Date : 2022-12-01 DOI: 10.1080/19336934.2022.2076539

Yutaka Matsubayashi

引用次数: 0

Notch Signalling Under Maternal-to-Zygotic Transition. 母系转合子过程中的Notch信号传导。

IF 1.2 4区生物学

Fly Pub Date : 2022-12-01 DOI: 10.1080/19336934.2022.2139981

Tomoko Yamakawa, Elzava Yuslimatin Mujizah, Kenji Matsuno

{"title":"Notch Signalling Under Maternal-to-Zygotic Transition.","authors":"Tomoko Yamakawa, Elzava Yuslimatin Mujizah, Kenji Matsuno","doi":"10.1080/19336934.2022.2139981","DOIUrl":"https://doi.org/10.1080/19336934.2022.2139981","url":null,"abstract":"The development of all animal embryos is initially directed by the gene products supplied by their mothers. With the progression of embryogenesis, the embryo's genome is activated to command subsequent developments. This transition, which has been studied in many model animals, is referred to as the Maternal-to-Zygotic Transition (MZT). In many organisms, including flies, nematodes, and sea urchins, genes involved in Notch signaling are extensively influenced by the MZT. This signaling pathway is highly conserved across metazoans; moreover, it regulates various developmental processes. Notch signaling defects are commonly associated with various human diseases. The maternal contribution of its factors was first discovered in flies. Subsequently, several genes were identified from mutant embryos with a phenotype similar to Notch mutants only upon the removal of the maternal contributions. Studies on these maternal genes have revealed various novel steps in the cascade of Notch signal transduction. Among these genes, pecanex and almondex have been functionally characterized in recent studies. Therefore, in this review, we will focus on the roles of these two maternal genes in Notch signaling and discuss future research directions on its maternal function.","PeriodicalId":12128,"journal":{"name":"Fly","volume":"16 1","pages":"347-359"},"PeriodicalIF":1.2,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9645253/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10472596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Characterization of enhancer fragments in Drosophila robo2. 果蝇 robo2 中增强子片段的特征。

IF 2.4 4区生物学

Fly Pub Date : 2022-12-01 DOI: 10.1080/19336934.2022.2126259

Gina Hauptman, Marie C Reichert, Muna A Abdal Rhida, Timothy A Evans

{"title":"Characterization of enhancer fragments in Drosophila robo2.","authors":"Gina Hauptman, Marie C Reichert, Muna A Abdal Rhida, Timothy A Evans","doi":"10.1080/19336934.2022.2126259","DOIUrl":"10.1080/19336934.2022.2126259","url":null,"abstract":"Receptor proteins of the Roundabout (Robo) family regulate axon guidance decisions during nervous system development. Among the three Drosophila robo family genes (robo1, robo2 and robo3), robo2 displays a dynamic expression pattern and regulates multiple axon guidance outcomes, including preventing midline crossing in some axons, promoting midline crossing in others, forming lateral longitudinal axon pathways, and regulating motor axon guidance. The identity and location of enhancer elements regulating robo2's complex and dynamic expression pattern in different neural cell types are unknown. Here, we characterize a set of 17 transgenic lines expressing GAL4 under the control of DNA sequences derived from noncoding regions in and around robo2, to identify enhancers controlling specific aspects of robo2 expression in the embryonic ventral nerve cord. We identify individual fragments that confer expression in specific cell types where robo2 is known to function, including early pioneer neurons, midline glia and lateral longitudinal neurons. Our results indicate that robo2's dynamic expression pattern is specified by a combination of enhancer elements that are active in different subsets of cells. We show that robo2's expression in lateral longitudinal axons represents two genetically separable subsets of neurons, and compare their axon projections with each other and with Fasciclin II (FasII), a commonly used marker of longitudinal axon pathways. In addition, we provide a general description of each fragment's expression in embryonic tissues outside of the nervous system, to serve as a resource for other researchers interested in robo2 expression and its functional roles outside the central nervous system.","PeriodicalId":12128,"journal":{"name":"Fly","volume":"16 1","pages":"312-346"},"PeriodicalIF":2.4,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/3b/61/KFLY_16_2126259.PMC9559326.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10478241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cellular mechanisms underlying adult tissue plasticity in Drosophila. 果蝇成体组织可塑性的细胞机制。

IF 1.2 4区生物学

Fly Pub Date : 2022-12-01 DOI: 10.1080/19336934.2022.2066952

Hiroki Nagai, Masayuki Miura, Yu-Ichiro Nakajima

{"title":"Cellular mechanisms underlying adult tissue plasticity in Drosophila.","authors":"Hiroki Nagai, Masayuki Miura, Yu-Ichiro Nakajima","doi":"10.1080/19336934.2022.2066952","DOIUrl":"https://doi.org/10.1080/19336934.2022.2066952","url":null,"abstract":"Adult tissues in Metazoa dynamically remodel their structures in response to environmental challenges including sudden injury, pathogen infection, and nutritional fluctuation, while maintaining quiescence under homoeostatic conditions. This characteristic, hereafter referred to as adult tissue plasticity, can prevent tissue dysfunction and improve the fitness of organisms in continuous and/or severe change of environments. With its relatively simple tissue structures and genetic tools, studies using the fruit fly Drosophila melanogaster have provided insights into molecular mechanisms that control cellular responses, particularly during regeneration and nutrient adaptation. In this review, we present the current understanding of cellular mechanisms, stem cell proliferation, polyploidization, and cell fate plasticity, all of which enable adult tissue plasticity in various Drosophila adult organs including the midgut, the brain, and the gonad, and discuss the organismal strategy in response to environmental changes and future directions of the research.","PeriodicalId":12128,"journal":{"name":"Fly","volume":"16 1","pages":"190-206"},"PeriodicalIF":1.2,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9045823/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9546157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

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