IF 1.4 4区生物学

Fly Pub Date : 2026-12-01 Epub Date: 2026-01-16 DOI: 10.1080/19336934.2026.2616950

Benjamin Geier, Logan Neely, Eli Coronado, Lawrence T Reiter

{"title":"Loss of Drosophila UBE3A phenocopies Piezo dysfunction and drives hyperphagic feeding in Drosophila.","authors":"Benjamin Geier, Logan Neely, Eli Coronado, Lawrence T Reiter","doi":"10.1080/19336934.2026.2616950","DOIUrl":"10.1080/19336934.2026.2616950","url":null,"abstract":"Angelman syndrome (AS) is a rare neurogenetic disorder characterized by developmental delay, speech impairment, ataxia, epilepsy, and in some cases hyperphagic feeding behavior. AS is caused by loss of function mutations, loss of expression, or maternal allele deletion of the E3 ubiquitin ligase UBE3A. Recent work has identified a connection between UBE3A and the mechanosensitive ion channel PIEZO2, raising the possibility that UBE3A may regulate PIEZO-dependent satiety signaling. In this study, we investigated the role of the Drosophila UBE3A ortholog, Dube3a, in Piezo-associated feeding behaviors. Single-cell RNA-sequencing data revealed overlapping expression of Dube3a and Piezo within crop and enterocyte populations of the gut, identifying a relevant cellular context for this pathway to occur. We developed a novel feeding assay using GFP-expressing yeast to quantify food intake and gut distention in vivo. Dube3a loss-of-function (Dube3a15b) flies exhibited hyperphagia and gut distention nearly identical to Piezo knockout flies. Analysis of chromosomal deficiency lines spanning the Dube3a locus further supported a requirement for Dube3a in normal satiety signaling. Finally, biochemical analyses demonstrated that Dube3a knockdown results in decreased Piezo protein levels, consistent with an indirect regulatory relationship. Together, these findings identify Dube3a as a critical regulator of Piezo-dependent satiety pathways and suggest that dysregulation of mechanosensory signaling may contribute to hyperphagia observed in AS. Further work is needed to define the intermediate factors linking UBE3A activity to Piezo stability and function.","PeriodicalId":12128,"journal":{"name":"Fly","volume":"20 1","pages":"2616950"},"PeriodicalIF":1.4,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12818797/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145988932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Systematic generation of Drosophila Wnt transgenes enables the characterization of canonical Wnt signaling. 果蝇Wnt转基因的系统生成使典型Wnt信号的表征成为可能。

IF 1.4 4区生物学

Fly Pub Date : 2026-12-01 Epub Date: 2026-02-10 DOI: 10.1080/19336934.2026.2624185

Jamie C Little, Marc Debrunner, Thom de Hoog, Farzad Tadjik, Chiara J Kehl, George Hausmann, Erich Brunner, Konrad Basler

{"title":"Systematic generation of Drosophila Wnt transgenes enables the characterization of canonical Wnt signaling.","authors":"Jamie C Little, Marc Debrunner, Thom de Hoog, Farzad Tadjik, Chiara J Kehl, George Hausmann, Erich Brunner, Konrad Basler","doi":"10.1080/19336934.2026.2624185","DOIUrl":"10.1080/19336934.2026.2624185","url":null,"abstract":"A conserved cohort of signalling pathways orchestrate development and adult homoeostasis. Deregulation of these pathways underlies many diseases. A key set of signals is the family of Wnt ligands. Members of this family are conserved, but a clear understanding of the unique and redundant roles is lacking. Previous efforts to study Wnt ligand function in Drosophila have been hampered by the difficulty of generating , functional transgenes. To address this, we have created a complete set of synthesized constructs in an insulated expression system, integrated into the same genomic location, enabling reliable gain-of-function analyses across multiple tissues. Distinct phenotypic outcomes were observed, reflecting both shared and unique features of individual ligands. To define the canonicity of Wnt signalling, we monitored canonical targets such as Dfz3, notum, and the 'naked cuticle' phenotype in developing tissues and the adult gut. Our findings revealed strong evidence of canonical responses from not only wg, but also DWnt6 and DWnt10 in the embryo, wing disc, larval gut, and adult gut. In addition, DWnt2, DWnt4, and WntD produced phenotypes distinct from the control with DWnt2 and DWnt4, showing context-dependent evidence of some canonical activity. While previous studies have suggested regulatory features between wg and DWnt6, our work provides functional evidence that Wg, DWnt6, and DWnt10 each induce expression of canonical signalling reporters in vivo. These findings refine our understanding of redundancy and specificity within the Drosophila Wnt family and demonstrate that multiple Wnt ligands can act similarly within the canonical pathway depending on tissue context.","PeriodicalId":12128,"journal":{"name":"Fly","volume":"20 1","pages":"2624185"},"PeriodicalIF":1.4,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12893696/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146156421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Toxicological effects of polypropylene microplastics and co-exposure with arsenic in Drosophila melanogaster. 聚丙烯微塑料及其与砷共暴露对黑腹果蝇的毒理学影响。

IF 1.4 4区生物学

Fly Pub Date : 2026-12-01 Epub Date: 2026-07-30 DOI: 10.1080/19336934.2026.2707706

María Llasbeth Hernández-Calderón, Alondra Gallegos-Moreno, Aneet Yamely Miranda-Camacho, David Ramiro Aguillón-Gutiérrez, Sandra Díaz Barriga-Arceo

{"title":"Toxicological effects of polypropylene microplastics and co-exposure with arsenic in Drosophila melanogaster.","authors":"María Llasbeth Hernández-Calderón, Alondra Gallegos-Moreno, Aneet Yamely Miranda-Camacho, David Ramiro Aguillón-Gutiérrez, Sandra Díaz Barriga-Arceo","doi":"10.1080/19336934.2026.2707706","DOIUrl":"10.1080/19336934.2026.2707706","url":null,"abstract":"Microplastics (MPs) are particles smaller than 5 mm that, due to their physicochemical properties, can act as carriers for contaminants such as heavy metals. In this study, we evaluated the toxicological effects of polypropylene microplastics (PP-MPs) and their co-exposure with dimethylarsinic acid (DMA) in the Drosophila melanogaster model. Third-instar larvae were exposed to the following experimental groups: negative control, positive control (0.02% H2O2), cosolvent control (ethanol, 6.94% final concentration), PP-MPs (0.01 g/mL), DMA (0.5 mM), and the co-exposure group (PP-MPs + DMA). Larval-to-adult viability, morphology, tissue damage, and fecundity were assessed. PP-MPs alone did not significantly affect larval-to-adult viability, the incidence of morphological abnormalities, or fecundity; however, evidence of tissue damage was observed. In contrast, co-exposure suggested an antagonistic effect on larval-to-adult viability and tissue damage, while indicating a synergistic effect on fecundity and the incidence of abnormalities. These findings contribute to a better understanding of the biological effects associated with co-exposure to microplastics and arsenic in an in vivo model.","PeriodicalId":12128,"journal":{"name":"Fly","volume":"20 1","pages":"2707706"},"PeriodicalIF":1.4,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13432825/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148619031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Drosophila as a model in organophosphate toxicology. 果蝇作为有机磷毒理学的模型。

IF 1.4 4区生物学

Fly Pub Date : 2026-12-01 Epub Date: 2026-06-27 DOI: 10.1080/19336934.2026.2695497

Marta Tkachuk, Nataliya Matiytsiv

引用次数: 0

Generation of full-length wild-type and mutated futsch transgenes in Drosophila-efficient Gibson Assembly of ultra-large cDNAs. 在果蝇高效的超大型cdna的Gibson组装中产生全长野生型和突变的futsch转基因。

IF 1.4 4区生物学

Fly Pub Date : 2026-12-01 Epub Date: 2026-09-04 DOI: 10.1080/19336934.2026.2719251

Chunlai Wu

{"title":"Generation of full-length wild-type and mutated futsch transgenes in Drosophila-efficient Gibson Assembly of ultra-large cDNAs.","authors":"Chunlai Wu","doi":"10.1080/19336934.2026.2719251","DOIUrl":"10.1080/19336934.2026.2719251","url":null,"abstract":"Drosophila Futsch is a key microtubule-associated protein (fly homolog of MAP1B) that regulates microtubule organization, synaptic terminal growth, and neuronal development. Functional analysis of futsch has long been limited by the inability to clone and express a full-length futsch transgene, owing to its exceptional size (~16.5 kb) and extensive repetitive sequences. Here, I present an efficient and reproducible method for cloning both wild-type and mutated full-length Drosophila futsch cDNA (16,488 bp) using Gibson Assembly. These resulting cDNAs were used to generate UAS-futsch transgenes. When expressed in neurons, the wild‑type transgenic Futsch associated with microtubule and rescued the synaptic morphological defects observed in futschK68 mutants. This approach substantially reduces the time and complexity compared with traditional cloning techniques. Furthermore, I highlight common pitfalls encountered during the cloning process and provide practical solutions to enhance cloning efficiency. This protocol offers a broadly applicable and cost-effective framework for cloning otherwise intractable large cDNAs from Drosophila and other organisms.","PeriodicalId":12128,"journal":{"name":"Fly","volume":"20 1","pages":"2719251"},"PeriodicalIF":1.4,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13556936/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148891410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring the versatility of Drosophila melanogaster as a model organism in biomedical research: a comprehensive review. 探索黑腹果蝇作为模式生物在生物医学研究中的多功能性：综述。

IF 2.2 4区生物学

Fly Pub Date : 2025-12-01 Epub Date: 2024-12-25 DOI: 10.1080/19336934.2024.2420453

Ayomide Victor Atoki, Patrick Maduabuchi Aja, Tijjani Salihu Shinkafi, Erick Nyakundi Ondari, Adekunle Ismahil Adeniyi, Ilemobayo Victor Fasogbon, Reuben Samson Dangana, Umar Uthman Shehu, Akinpelumi Akin-Adewumi

{"title":"Exploring the versatility of Drosophila melanogaster as a model organism in biomedical research: a comprehensive review.","authors":"Ayomide Victor Atoki, Patrick Maduabuchi Aja, Tijjani Salihu Shinkafi, Erick Nyakundi Ondari, Adekunle Ismahil Adeniyi, Ilemobayo Victor Fasogbon, Reuben Samson Dangana, Umar Uthman Shehu, Akinpelumi Akin-Adewumi","doi":"10.1080/19336934.2024.2420453","DOIUrl":"10.1080/19336934.2024.2420453","url":null,"abstract":"Drosophila melanogaster is a highly versatile model organism that has profoundly advanced our understanding of human diseases. With more than 60% of its genes having human homologs, Drosophila provides an invaluable system for modelling a wide range of pathologies, including neurodegenerative disorders, cancer, metabolic diseases, as well as cardiac and muscular conditions. This review highlights key developments in utilizing Drosophila for disease modelling, emphasizing the genetic tools that have transformed research in this field. Technologies such as the GAL4/UAS system, RNA interference (RNAi) and CRISPR-Cas9 have enabled precise genetic manipulation, with CRISPR-Cas9 allowing for the introduction of human disease mutations into orthologous Drosophila genes. These approaches have yielded critical insights into disease mechanisms, identified novel therapeutic targets and facilitated both drug screening and toxicological studies. Articles were selected based on their relevance, impact and contribution to the field, with a particular focus on studies offering innovative perspectives on disease mechanisms or therapeutic strategies. Our findings emphasize the central role of Drosophila in studying complex human diseases, underscoring its genetic similarities to humans and its effectiveness in modelling conditions such as Alzheimer's disease, Parkinson's disease and cancer. This review reaffirms Drosophila's critical role as a model organism, highlighting its potential to drive future research and therapeutic advancements.","PeriodicalId":12128,"journal":{"name":"Fly","volume":"19 1","pages":"2420453"},"PeriodicalIF":2.2,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11702942/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142893246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

UCH-L1 regulates eye differentiation-related genes and modulates EGFR signalling in Drosophila melanogaster. UCH-L1调节黑腹果蝇眼分化相关基因并调节EGFR信号传导。

IF 2.2 4区生物学

Fly Pub Date : 2025-12-01 Epub Date: 2025-11-13 DOI: 10.1080/19336934.2025.2580003

Nguyen Anh Tuan, Tran Linh Thuoc, Dang Thi Phuong Thao

{"title":"UCH-L1 regulates eye differentiation-related genes and modulates EGFR signalling in Drosophila melanogaster.","authors":"Nguyen Anh Tuan, Tran Linh Thuoc, Dang Thi Phuong Thao","doi":"10.1080/19336934.2025.2580003","DOIUrl":"10.1080/19336934.2025.2580003","url":null,"abstract":"UCH-L1 (Ubiquitin Carboxyl-terminal Hydrolase - L1) is a protein that plays a critical role in the ubiquitin-proteasome system. Previous studies have demonstrated a link between UCH-L1 and various diseases, including neurodegenerative disorders, diabetes, and cancer. However, the role of UCH-L1 in development remains unclear. To investigate the functions of UCH-L1 in a living organism, taking advantage of the Drosophila model, and to explore the correlation between Drosophila UCH (dUCH) and human UCH-L1, we established a GAL4/UAS-targeted expression system to examine the effect of dUCH on Drosophila eye development. We found that knockdown of dUCH resulted in a rough eye phenotype associated with the MAPK pathway. In this study, for the first time, we revealed that loss of dUCH function leads to a reduction in EGFR protein levels. Additionally, dUCH knockdown downregulated Spitz (spi), a ligand of EGFR, as well as Draf, a key component of the MAPK pathway. Furthermore, under dUCH knockdown conditions, several genes known to play critical roles in eye cell differentiation were affected, including the downregulation of sens, salm, lz, barth1/2, and salm, which are essential for the differentiation of R2/5, R3/4, and R1/6 photoreceptor cells. Interestingly, dUCH was found to be involved not only in the MAPK pathway but also in the regulation of pros, lz, barth1/2, and sev gene expression, suggesting its role in R7 photoreceptor differentiation. Taken together, these findings highlight the important role of dUCH in regulating genes associated with eye cell differentiation and its involvement in EGFR signalling in Drosophila melanogaster.","PeriodicalId":12128,"journal":{"name":"Fly","volume":"19 1","pages":"2580003"},"PeriodicalIF":2.2,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12622323/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145511784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Innate immunity pathways activate cell proliferation after penetrating traumatic brain injury in adult Drosophila. 先天性免疫途径激活成年果蝇穿透性创伤性脑损伤后的细胞增殖。

IF 2.2 4区生物学

Fly Pub Date : 2025-12-01 Epub Date: 2025-11-14 DOI: 10.1080/19336934.2025.2586357

Shawn Ahern-Djamali, Khailee Marischuk, Kassi L Crocker, Isabella Peetz, Eli Scott, Grace Boekhoff-Falk

{"title":"Innate immunity pathways activate cell proliferation after penetrating traumatic brain injury in adult Drosophila.","authors":"Shawn Ahern-Djamali, Khailee Marischuk, Kassi L Crocker, Isabella Peetz, Eli Scott, Grace Boekhoff-Falk","doi":"10.1080/19336934.2025.2586357","DOIUrl":"10.1080/19336934.2025.2586357","url":null,"abstract":"We are utilizing an adult penetrating traumatic brain injury (PTBI) model in Drosophila to investigate regenerative mechanisms after damage to the central brain. Here, we focus on cell proliferation as an early event in the regenerative process. To identify pathways that could trigger cell proliferation following PTBI, we utilized bulk RNA-Seq. We find that transcript levels for components of both Toll and Immune Deficiency (Imd) innate immunity pathways are rapidly and highly upregulated post-PTBI. We then tested mutants for the NF-κB transcription factors of the Toll and Imd pathways, Dorsal-related immunity factor (Dif) and Relish (Rel), respectively. We find that loss of either Dif or Rel results in loss of cell proliferation after injury and identify tissue-specific requirements for Dif and Rel. In addition, while the canonical downstream targets of Drosophila innate immune signalling, the antimicrobial peptides (AMPs), are upregulated following PTBI, their levels revert to near baseline within 24 hr. Taken together, these results indicate that the innate immunity pathways play an integral role in the regenerative response and that this response may not require the antimicrobial peptides. Innate immunity previously has been implicated as both a potentiator and an inhibitor of regenerative processes. Our work suggests that modulation of innate immunity may be essential to prevent adverse outcomes. Thus, this work is likely to inform future experiments to dissect regenerative mechanisms in higher organisms as well as in Drosophila.","PeriodicalId":12128,"journal":{"name":"Fly","volume":"19 1","pages":"2586357"},"PeriodicalIF":2.2,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12622319/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145511854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

What the fruit fly can tell us about autosomal recessive primary microcephaly. 果蝇能告诉我们的常染色体隐性原发性小头症。

IF 1.4 4区生物学

Fly Pub Date : 2025-12-01 Epub Date: 2025-10-11 DOI: 10.1080/19336934.2025.2572866

Shalini Chakraborty, Steven Florez, Todd Schoborg

{"title":"What the fruit fly can tell us about autosomal recessive primary microcephaly.","authors":"Shalini Chakraborty, Steven Florez, Todd Schoborg","doi":"10.1080/19336934.2025.2572866","DOIUrl":"10.1080/19336934.2025.2572866","url":null,"abstract":"Three decades of research aimed at understanding the basis for autosomal recessive primary microcephaly (MCPH), a human clinical disorder defined by a significant reduction in head and brain size, has uncovered a suite of ~30 genes that participate in this process. Work in both vertebrate and invertebrate model systems have been instrumental in attempting to link MCPH gene function to the brain growth phenotype. However, we still lack definitive evidence as to what these functions are for many of these genes. In this review, we summarize recent work in Drosophila aimed at overcoming these limitations in our knowledge of MCPH gene function that may be applicable to humans. We discuss the clinical features of MCPH, parallels between human and Drosophila neurogenesis modes with a particular focus on the fly optic lobe, and highlight four of the most well-studied Drosophila MCPH orthologs: abnormal spindle (asp)/MCPH5, Microcephalin/MCPH1, WD Repeat-Containing Protein 62 (Wdr62)/MCPH2, and Ankryin Repeat-and LEM Domain- Containing Protein 2 (ANKLE2)/MCPH16. We focus on the multifunctional roles for these proteins that may underlie the microcephaly phenotype and advocate for the use of flies as a relevant model for human MCPH.","PeriodicalId":12128,"journal":{"name":"Fly","volume":"19 1","pages":"2572866"},"PeriodicalIF":1.4,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12520113/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145274250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Stage-specific modulation of Drosophila gene expression with muscle GAL4 promoters. 肌肉GAL4启动子对果蝇基因表达的阶段性调控。

IF 2.2 4区生物学

Fly Pub Date : 2025-12-01 Epub Date: 2025-01-07 DOI: 10.1080/19336934.2024.2447617

Ziwei Zhao, Erika R Geisbrecht

引用次数: 0

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