IF 1.2 4区生物学

Fly Pub Date : 2022-12-01 DOI: 10.1080/19336934.2022.2030191

Geanette Lam, Katherine Beebe, Carl S Thummel

{"title":"A direct-drive GFP reporter for studies of tracheal development in Drosophila.","authors":"Geanette Lam, Katherine Beebe, Carl S Thummel","doi":"10.1080/19336934.2022.2030191","DOIUrl":"https://doi.org/10.1080/19336934.2022.2030191","url":null,"abstract":"The Drosophila tracheal system consists of a widespread tubular network that provides respiratory functions for the animal. Its development, from ten pairs of placodes in the embryo to the final stereotypical branched structure in the adult, has been extensively studied by many labs as a model system for understanding tubular epithelial morphogenesis. Throughout these studies, a breathless (btl)-GAL4 driver has provided an invaluable tool to either mark tracheal cells during development or to manipulate gene expression in this tissue. A distinct shortcoming of this approach, however, is that btl-GAL4 cannot be used to specifically visualize tracheal cells in the presence of other GAL4 drivers or other UAS constructs, restricting its utility. Here we describe a direct-drive btl-nGFP reporter that can be used as a specific marker of tracheal cells throughout development in combination with any GAL4 driver and/or UAS construct. This reporter line should facilitate the use of Drosophila as a model system for studies of tracheal development and tubular morphogenesis.","PeriodicalId":12128,"journal":{"name":"Fly","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8803062/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39870583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Patched and Costal-2 mutations lead to differences in tissue overgrowth autonomy. Patched和Costal-2突变导致组织过度生长自主性的差异。

IF 2.4 4区生物学

Fly Pub Date : 2022-12-01 DOI: 10.1080/19336934.2022.2062991

Shannon L Moore, Frank C Adamini, Erik S Coopes, Dustin Godoy, Shyra J Northington, Jordan M Stewart, Richard L Tillett, Kayla L Bieser, Jacob D Kagey

{"title":"Patched and Costal-2 mutations lead to differences in tissue overgrowth autonomy.","authors":"Shannon L Moore, Frank C Adamini, Erik S Coopes, Dustin Godoy, Shyra J Northington, Jordan M Stewart, Richard L Tillett, Kayla L Bieser, Jacob D Kagey","doi":"10.1080/19336934.2022.2062991","DOIUrl":"10.1080/19336934.2022.2062991","url":null,"abstract":"Genetic screens are used in Drosophila melanogaster to identify genes key in the regulation of organismal development and growth. These screens have defined signalling pathways necessary for tissue and organismal development, which are evolutionarily conserved across species, including Drosophila. Here, we have used an FLP/FRT mosaic system to screen for conditional regulators of cell growth and cell division in the Drosophila eye. The conditional nature of this screen utilizes a block in the apoptotic pathway to prohibit the mosaic mutant cells from dying via apoptosis. From this screen, we identified two different mutants that mapped to the Hedgehog signalling pathway. Previously, we described a novel Ptc mutation and here we add to the understanding of disrupting the Hh pathway with a novel allele of Cos2. Both of these Hh components are negative regulators of the pathway, yet they depict mutant differences in the type of overgrowth created. Ptc mutations lead to overgrowth consisting of almost entirely wild-type tissue (non-autonomous overgrowth), while the Cos2 mutation results in tissue that is overgrown in both the mutant and wild-type clones (both autonomous and non-autonomous). These differences in tissue overgrowth are consistent in the Drosophila eye and wing. The observed difference is correlated with different deregulation patterns of pMad, the downstream effector of DPP signalling. This finding provides insight into pathway-specific differences that help to better understand intricacies of developmental processes and human diseases that result from deregulated Hedgehog signalling, such as basal cell carcinoma.","PeriodicalId":12128,"journal":{"name":"Fly","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9045829/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10603000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spatiotemporal changes in microtubule dynamics during dendritic morphogenesis. 树突形态发生过程中微管动力学的时空变化

IF 2.4 4区生物学

Fly Pub Date : 2022-12-01 DOI: 10.1080/19336934.2021.1976033

Chun Hu, Pan Feng, Meilan Chen, Yan Tang, Peter Soba

{"title":"Spatiotemporal changes in microtubule dynamics during dendritic morphogenesis.","authors":"Chun Hu, Pan Feng, Meilan Chen, Yan Tang, Peter Soba","doi":"10.1080/19336934.2021.1976033","DOIUrl":"10.1080/19336934.2021.1976033","url":null,"abstract":"Dendritic morphogenesis requires dynamic microtubules (MTs) to form a coordinated cytoskeletal network during development. Dynamic MTs are characterized by their number, polarity and speed of polymerization. Previous studies described a correlation between anterograde MT growth and terminal branch extension in Drosophila dendritic arborization (da) neurons, suggesting a model that anterograde MT polymerization provides a driving force for dendritic branching. We recently found that the Ste20-like kinase Tao specifically regulates dendritic branching by controlling the number of dynamic MTs in a kinase activity-dependent fashion, without affecting MT polarity or speed. This finding raises the interesting question of how MT dynamics affects dendritic morphogenesis, and if Tao kinase activity is developmentally regulated to coordinate MT dynamics and dendritic morphogenesis. We explored the possible correlation between MT dynamics and dendritic morphogenesis together with the activity changes of Tao kinase in C1da and C4da neurons during larval development. Our data show that spatiotemporal changes in the number of dynamic MTs, but not polarity or polymerization speed, correlate with dendritic branching and Tao kinase activity. Our findings suggest that Tao kinase limits dendritic branching by controlling the abundance of dynamic MTs and we propose a novel model on how regulation of MT dynamics might influence dendritic morphogenesis.","PeriodicalId":12128,"journal":{"name":"Fly","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8496546/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9188952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mathematical modeling of Notch dynamics in Drosophila neural development. 果蝇神经发育过程中Notch动力学的数学建模。

IF 1.2 4区生物学

Fly Pub Date : 2022-12-01 DOI: 10.1080/19336934.2021.1953363

Tetsuo Yasugi, Makoto Sato

引用次数: 2

How Social Experience and Environment Impacts Behavioural Plasticity in Drosophila. 社会经验和环境如何影响果蝇的行为可塑性。

IF 1.2 4区生物学

Fly Pub Date : 2022-12-01 DOI: 10.1080/19336934.2021.1989248

Molly Chen, Marla B Sokolowski

引用次数: 4

Studies of neurodegenerative diseases using Drosophila and the development of novel approaches for their analysis. 利用果蝇研究神经退行性疾病，并开发新的分析方法。

IF 2.4 4区生物学

Fly Pub Date : 2022-12-01 DOI: 10.1080/19336934.2022.2087484

Yohei Nitta, Atsushi Sugie

引用次数: 0

Adaptation and ecological speciation in seasonally varying environments at high latitudes: Drosophila virilis group. 高纬度季节变化环境下的适应与生态物种形成:果蝇类群。

IF 1.2 4区生物学

Fly Pub Date : 2022-12-01 DOI: 10.1080/19336934.2021.2016327

Anneli Hoikkala, Noora Poikela

{"title":"Adaptation and ecological speciation in seasonally varying environments at high latitudes: Drosophila virilis group.","authors":"Anneli Hoikkala, Noora Poikela","doi":"10.1080/19336934.2021.2016327","DOIUrl":"https://doi.org/10.1080/19336934.2021.2016327","url":null,"abstract":"Living in high latitudes and altitudes sets specific requirements on species' ability to forecast seasonal changes and to respond to them in an appropriate way. Adaptation into diverse environmental conditions can also lead to ecological speciation through habitat isolation or by inducing changes in traits that influence assortative mating. In this review, we explain how the unique time-measuring systems of Drosophila virilis group species have enabled the species to occupy high latitudes and how the traits involved in species reproduction and survival exhibit strong linkage with latitudinally varying photoperiodic and climatic conditions. We also describe variation in reproductive barriers between the populations of two species with overlapping distributions and show how local adaptation and the reinforcement of prezygotic barriers have created partial reproductive isolation between conspecific populations. Finally, we consider the role of species-specific chromosomal inversions and the X chromosome in the development of reproductive barriers between diverging lineages.","PeriodicalId":12128,"journal":{"name":"Fly","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8786326/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39846841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Ancestral dietary change alters the development of Drosophila larvae through MAPK signalling. 祖先的饮食改变通过MAPK信号改变果蝇幼虫的发育。

IF 1.2 4区生物学

Fly Pub Date : 2022-12-01 DOI: 10.1080/19336934.2022.2088032

Samuel G Towarnicki, Neil A Youngson, Susan M Corley, Jus C St John, Richard G Melvin, Nigel Turner, Margaret J Morris, J William O Ballard

{"title":"Ancestral dietary change alters the development of Drosophila larvae through MAPK signalling.","authors":"Samuel G Towarnicki, Neil A Youngson, Susan M Corley, Jus C St John, Richard G Melvin, Nigel Turner, Margaret J Morris, J William O Ballard","doi":"10.1080/19336934.2022.2088032","DOIUrl":"https://doi.org/10.1080/19336934.2022.2088032","url":null,"abstract":"Studies in a broad range of animal species have revealed phenotypes that are caused by ancestral life experiences, including stress and diet. Ancestral dietary macronutrient composition and quantity (over- and under-nutrition) have been shown to alter descendent growth, metabolism and behaviour. Molecules have been identified in gametes that are changed by ancestral diet and are required for transgenerational effects. However, there is less understanding of the developmental pathways altered by inherited molecules during the period between fertilization and adulthood. To investigate this non-genetic inheritance, we exposed great grand-parental and grand-parental generations to defined protein to carbohydrate (P:C) dietary ratios. Descendent developmental timing was consistently faster in the period between the embryonic and pupal stages when ancestors had a higher P:C ratio diet. Transcriptional analysis revealed extensive and long-lasting changes to the MAPK signalling pathway, which controls growth rate through the regulation of ribosomal RNA transcription. Pharmacological inhibition of both MAPK and rRNA pathways recapitulated the ancestral diet-induced developmental changes. This work provides insight into non-genetic inheritance between fertilization and adulthood.","PeriodicalId":12128,"journal":{"name":"Fly","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9354765/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10528622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

A robust method to isolate Drosophila fat body nuclei for transcriptomic analysis. 一种分离果蝇脂肪体核进行转录组学分析的可靠方法。

IF 1.2 4区生物学

Fly Pub Date : 2022-12-01 DOI: 10.1080/19336934.2021.1978776

Vanika Gupta, Brian P Lazzaro

{"title":"A robust method to isolate Drosophila fat body nuclei for transcriptomic analysis.","authors":"Vanika Gupta, Brian P Lazzaro","doi":"10.1080/19336934.2021.1978776","DOIUrl":"https://doi.org/10.1080/19336934.2021.1978776","url":null,"abstract":"Gene expression profiles are typically described at the level of the tissue or, often in Drosophila, at the level of the whole organism. Collapsing the gene expression of entire tissues into single measures averages over potentially important heterogeneity among the cells that make up that tissue. The advent of single-cell RNA-sequencing technology (sc-RNAseq) allows transcriptomic evaluation of the individual cells that make up a tissue. However, sc-RNAseq requires a high-quality suspension of viable cells or nuclei, and cell dissociation methods that yield healthy cells and nuclei are still lacking for many important tissues. The insect fat body is a polyfunctional tissue responsible for diverse physiological processes and therefore is an important target for sc-RNAseq. The Drosophila adult fat body consists of fragile cells that are difficult to dissociate while maintaining cell viability. As an alternative, we developed a method to isolate single fat body nuclei for RNA-seq. Our isolation method is largely free of mitochondrial contamination and yields higher capture of transcripts per nucleus compared to other nuclei preparation methods. Our method works well for single-cell nuclei sequencing and can potentially be implemented for bulk RNA-seq.","PeriodicalId":12128,"journal":{"name":"Fly","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8500699/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39488730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

HaloTag-based reporters for sparse labeling and cell tracking. 基于halotag的报告器，用于稀疏标记和细胞跟踪。

IF 1.2 4区生物学

Fly Pub Date : 2022-12-01 DOI: 10.1080/19336934.2022.2142460

Lydie Couturier, Juan Luna, Khalil Mazouni, Claire Mestdagh, Minh-Son Phan, Francis Corson, Francois Schweisguth

{"title":"HaloTag-based reporters for sparse labeling and cell tracking.","authors":"Lydie Couturier, Juan Luna, Khalil Mazouni, Claire Mestdagh, Minh-Son Phan, Francis Corson, Francois Schweisguth","doi":"10.1080/19336934.2022.2142460","DOIUrl":"https://doi.org/10.1080/19336934.2022.2142460","url":null,"abstract":"Multiscale analysis of morphogenesis requires to follow and measure in real-time the in vivo behaviour of large numbers of individual cells over long period of time. Despite recent progress, the large-scale automated tracking of cells in developing embryos and tissues remains a challenge. Here we describe a genetic tool for the random and sparse labelling of individual cells in developing Drosophila tissues. This tool is based on the conditional expression of a nuclear HaloTag protein that can be fluorescently labelled upon the irreversible binding of a cell permeable synthetic ligand. While the slow maturation of genetically encoded fluorescent renders the tracking of individual cells difficult in rapidly dividing tissues, nuclear HaloTag proteins allowed for rapid labelling of individual cells in cultured imaginal discs. To study cell shape changes, we also produced an HaloTag version of the actin-bound protein LifeAct. Since sparse labelling facilitates cell tracking, nuclear HaloTag reporters will be useful for the single-cell analysis of fate dynamics in Drosophila tissues cultured ex vivo.","PeriodicalId":12128,"journal":{"name":"Fly","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9635558/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10474465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fly最新文献