IF 1.2 4区生物学

Fly Pub Date : 2017-04-03 DOI: 10.1080/19336934.2016.1238993

Allison K Timmons, Albert A Mondragon, Tracy L. Meehan, K. McCall

{"title":"Control of non-apoptotic nurse cell death by engulfment genes in Drosophila","authors":"Allison K Timmons, Albert A Mondragon, Tracy L. Meehan, K. McCall","doi":"10.1080/19336934.2016.1238993","DOIUrl":"https://doi.org/10.1080/19336934.2016.1238993","url":null,"abstract":"ABSTRACT Programmed cell death occurs as a normal part of oocyte development in Drosophila. For each egg that is formed, 15 germline-derived nurse cells transfer their cytoplasmic contents into the oocyte and die. Disruption of apoptosis or autophagy only partially inhibits the death of the nurse cells, indicating that other mechanisms significantly contribute to nurse cell death. Recently, we demonstrated that the surrounding stretch follicle cells non-autonomously promote nurse cell death during late oogenesis and that phagocytosis genes including draper, ced-12, and the JNK pathway are crucial for this process. When phagocytosis genes are inhibited in the follicle cells, events specifically associated with death of the nurse cells are impaired. Death of the nurse cells is not completely blocked in draper mutants, suggesting that other engulfment receptors are involved. Indeed, we found that the integrin subunit, αPS3, is enriched on stretch follicle cells during late oogenesis and is required for elimination of the nurse cells. Moreover, double mutant analysis revealed that integrins act in parallel to draper. Death of nurse cells in the Drosophila ovary is a unique example of programmed cell death that is both non-apoptotic and non-cell autonomously controlled.","PeriodicalId":12128,"journal":{"name":"Fly","volume":"11 1","pages":"104 - 111 - 55"},"PeriodicalIF":1.2,"publicationDate":"2017-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/19336934.2016.1238993","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43353624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 16

Parsimony and complexity: Cell fate assignment in the developing Drosophila eye 简约与复杂:发育中的果蝇眼睛的细胞命运分配

IF 1.2 4区生物学

Fly Pub Date : 2017-02-23 DOI: 10.1080/19336934.2017.1291103

Y. Mavromatakis, A. Tomlinson

{"title":"Parsimony and complexity: Cell fate assignment in the developing Drosophila eye","authors":"Y. Mavromatakis, A. Tomlinson","doi":"10.1080/19336934.2017.1291103","DOIUrl":"https://doi.org/10.1080/19336934.2017.1291103","url":null,"abstract":"ABSTRACT The specification of the R7 photoreceptor in the Drosophila eye has become a classic model for understanding how cell fates are assigned in developing systems. R7 is derived from a group of cells that also gives rise to the R1/6 photoreceptor class and the non-photoreceptor cone cells. Our studies examine the signals and cellular information that direct each of these cell types. The cell fates are directed by the combined actions of the Receptor Tyrosine Kinase (RTK) and Notch (N) signaling pathways. The RTK pathway acts to remove the transcription factor Tramtrack (Ttk) which represses the photoreceptor fate. If a cell receives an RTK signal sufficient to remove Ttk then the photoreceptor fate is specified; if not, the cone cell fate results. If Ttk is removed from a cell and its N activity is high then it is specified as an R7, but if its N activity is low then it becomes an R1/6 class photoreceptor. Thus, a remarkably simple molecular code underlies the specification of the fates: 1. Ttk degraded or not: 2. N activity high or low. In the R1/6 and cone cell precursors the molecular codes are achieved with relative simplicity but in the R7 precursor, manifold interactions occur between the RTK and N pathways, and to-date we have identified 4 distinct roles played by N in R7 fate specification. In this review we detail this molecular complexity, and describe how the RTK/N pathway crosstalk eventually leads to the simple molecular code of Tramtrack removed and N activity high. Furthermore, we describe the role played by the transcription factor Lozenge (Lz) in directing retinal precursor fates, and how the RTK/N signals specify different retinal cell types depending on the presence or absence of Lz.","PeriodicalId":12128,"journal":{"name":"Fly","volume":"11 1","pages":"171 - 178"},"PeriodicalIF":1.2,"publicationDate":"2017-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/19336934.2017.1291103","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46832024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Gluconeogenesis: An ancient biochemical pathway with a new twist 糖异生:一个古老的生化途径与一个新的转折

IF 1.2 4区生物学

Fly Pub Date : 2017-02-07 DOI: 10.1080/19336934.2017.1283081

T. Miyamoto, H. Amrein

引用次数: 30

secHsp70 as a tool to approach amyloid-β42 and other extracellular amyloids secHsp70作为接近淀粉样蛋白-β42和其他细胞外淀粉样蛋白的工具

IF 1.2 4区生物学

Fly Pub Date : 2017-02-06 DOI: 10.1080/19336934.2017.1291104

Lorena de Mena, D. Chhangani, P. Fernandez-Funez, D. Rincon-Limas

{"title":"secHsp70 as a tool to approach amyloid-β42 and other extracellular amyloids","authors":"Lorena de Mena, D. Chhangani, P. Fernandez-Funez, D. Rincon-Limas","doi":"10.1080/19336934.2017.1291104","DOIUrl":"https://doi.org/10.1080/19336934.2017.1291104","url":null,"abstract":"ABSTRACT Self-association of amyloidogenic proteins is the main pathological trigger in a wide variety of neurodegenerative disorders. These aggregates are deposited inside or outside the cell due to hereditary mutations, environmental exposures or even normal aging. Cumulative evidence indicates that the heat shock chaperone Hsp70 possesses robust neuroprotection against various intracellular amyloids in Drosophila and mouse models. However, its protective role against extracellular amyloids was largely unknown as its presence outside the cells is very limited. Our recent manuscript in PNAS revealed that an engineered form of secreted Hsp70 (secHsp70) is highly protective against toxicity induced by extracellular deposition of the amyloid-β42 (Aβ42) peptide. In this Extra View article, we extend our analysis to other members of the heat shock protein family. We created PhiC31-based transgenic lines for human Hsp27, Hsp40, Hsp60 and Hsp70 and compared their activities in parallel against extracellular Aβ42. Strikingly, only secreted Hsp70 exhibits robust protection against Aβ42-triggered toxicity in the extracellular milieu. These observations indicate that the ability of secHsp70 to suppress Aβ42 insults is quite unique and suggest that targeted secretion of Hsp70 may represent a new therapeutic approach against Aβ42 and other extracellular amyloids. The potential applications of this engineered chaperone are discussed.","PeriodicalId":12128,"journal":{"name":"Fly","volume":"11 1","pages":"179 - 184"},"PeriodicalIF":1.2,"publicationDate":"2017-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/19336934.2017.1291104","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42649161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 18

Mobile-phone radiation-induced perturbation of gene-expression profiling, redox equilibrium and sporadic-apoptosis control in the ovary of Drosophila melanogaster 手机辐射对黑腹果蝇卵巢基因表达谱、氧化还原平衡和散发性细胞凋亡控制的干扰

IF 1.2 4区生物学

Fly Pub Date : 2017-01-12 DOI: 10.1080/19336934.2016.1270487

A. Manta, Deppie Papadopoulou, A. Polyzos, A. Fragopoulou, A. Skouroliakou, D. Thanos, D. Stravopodis, L. Margaritis

{"title":"Mobile-phone radiation-induced perturbation of gene-expression profiling, redox equilibrium and sporadic-apoptosis control in the ovary of Drosophila melanogaster","authors":"A. Manta, Deppie Papadopoulou, A. Polyzos, A. Fragopoulou, A. Skouroliakou, D. Thanos, D. Stravopodis, L. Margaritis","doi":"10.1080/19336934.2016.1270487","DOIUrl":"https://doi.org/10.1080/19336934.2016.1270487","url":null,"abstract":"ABSTRACT The daily use by people of wireless communication devices has increased exponentially in the last decade, begetting concerns regarding its potential health hazards. Drosophila melanogaster four days-old adult female flies were exposed for 30 min to radiation emitted by a commercial mobile phone at a SAR of 0.15 W/kg and a SAE of 270 J/kg. ROS levels and apoptotic follicles were assayed in parallel with a genome-wide microarrays analysis. ROS cellular contents were found to increase by 1.6-fold (x), immediately after the end of exposure, in follicles of pre-choriogenic stages (germarium - stage 10), while sporadically generated apoptotic follicles (germarium 2b and stages 7–9) presented with an averaged 2x upregulation in their sub-population mass, 4 h after fly's irradiation with mobile device. Microarray analysis revealed 168 genes being differentially expressed, 2 h post-exposure, in response to radiofrequency (RF) electromagnetic field-radiation exposure (≥1.25x, P < 0.05) and associated with multiple and critical biological processes, such as basic metabolism and cellular subroutines related to stress response and apoptotic death. Exposure of adult flies to mobile-phone radiation for 30 min has an immediate impact on ROS production in animal's ovary, which seems to cause a global, systemic and non-targeted transcriptional reprogramming of gene expression, 2 h post-exposure, being finally followed by induction of apoptosis 4 h after the end of exposure. Conclusively, this unique type of pulsed radiation, mainly being derived from daily used mobile phones, seems capable of mobilizing critical cytopathic mechanisms, and altering fundamental genetic programs and networks in D. melanogaster.","PeriodicalId":12128,"journal":{"name":"Fly","volume":"11 1","pages":"75 - 95"},"PeriodicalIF":1.2,"publicationDate":"2017-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/19336934.2016.1270487","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"60065301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 15

We also CanFly! The 2nd MexFly drosophila research conference 我们也会飞!第二届墨西哥果蝇研究会议

IF 1.2 4区生物学

Fly Pub Date : 2017-01-09 DOI: 10.1080/19336934.2016.1271517

F. Missirlis, M. Nahmad

引用次数: 0

How tissue damage MET metabolism: Regulation of the systemic damage response. 如何组织损伤MET代谢:调节全身损伤反应。

IF 1.2 4区生物学

Fly Pub Date : 2017-01-02 Epub Date: 2016-08-11 DOI: 10.1080/19336934.2016.1221549

Soshiro Kashio, Fumiaki Obata, Masayuki Miura

引用次数: 3

Drosophila yakuba mayottensis, a new model for the study of incipient ecological speciation. 雅库巴马约特果蝇:早期生态物种形成研究的新模式。

IF 1.2 4区生物学

Fly Pub Date : 2017-01-02 Epub Date: 2016-08-11 DOI: 10.1080/19336934.2016.1221550

Amir Yassin

{"title":"Drosophila yakuba mayottensis, a new model for the study of incipient ecological speciation.","authors":"Amir Yassin","doi":"10.1080/19336934.2016.1221550","DOIUrl":"https://doi.org/10.1080/19336934.2016.1221550","url":null,"abstract":"A full understanding of how ecological factors drive the fixation of genetic changes during speciation is obscured by the lack of appropriate models with clear natural history and powerful genetic toolkits. In a recent study, we described an early stage of ecological speciation in a population of the generalist species Drosophila yakuba (melanogaster subgroup) on the island of Mayotte (Indian Ocean). On this island, flies are strongly associated with the toxic fruits of noni (Morinda citrifolia) and show a partial degree of pre-zygotic reproductive isolation. Here, I mine the nuclear and mitochondrial genomes and provide a full morphological description of this population. Only 29 nuclear sites (< 4 × 10-7 of the genome) are fixed in this population and absent from 3 mainland populations and the closest relative D. santomea, but no mitochondrial or morphological character distinguish Mayotte flies from the mainland. This result indicates that physiological and behavioral traits may evolve faster than morphology at the early stages of speciation. Based on these differences, the Mayotte population is designated as a new subspecies, Drosophila yakuba mayottensis subsp. nov., and its strong potential in understanding the genetics of speciation and plant-insect interactions is discussed.","PeriodicalId":12128,"journal":{"name":"Fly","volume":"11 1","pages":"37-45"},"PeriodicalIF":1.2,"publicationDate":"2017-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/19336934.2016.1221550","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34389019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

The beneficial role of extracellular reactive oxygen species in apoptosis-induced compensatory proliferation. 细胞外活性氧在凋亡诱导的代偿性增殖中的有益作用。

IF 1.2 4区生物学

Fly Pub Date : 2017-01-02 Epub Date: 2016-08-15 DOI: 10.1080/19336934.2016.1222997

Neha Diwanji, Andreas Bergmann

{"title":"The beneficial role of extracellular reactive oxygen species in apoptosis-induced compensatory proliferation.","authors":"Neha Diwanji, Andreas Bergmann","doi":"10.1080/19336934.2016.1222997","DOIUrl":"https://doi.org/10.1080/19336934.2016.1222997","url":null,"abstract":"Apoptosis-induced proliferation (AiP) maintains tissue homeostasis following massive stress-induced cell death. During this phenomenon, dying cells induce proliferation of the surviving cells to compensate for the tissue loss, and thus restore organ size. Along with wound healing and tissue regeneration, AiP also contributes to tumor repopulation following radiation or chemotherapy. There are several models of AiP. Using an \"undead\" AiP model that causes hyperplastic overgrowth of Drosophila epithelial tissue, we recently demonstrated that extracellular reactive oxygen species (eROS) are produced by undead epithelial cells, and are necessary for inducing AiP and overgrowth. Furthermore, hemocytes, the Drosophila blood cells, are seen adjacent to the undead epithelial tissue, and may secrete the TNF ortholog Eiger that signals through the TNF receptor to active Jun-N-terminal kinase (JNK) in the undead tissue and induce proliferation. We propose that undead epithelial tissue triggers an inflammatory response that resembles recruitment of macrophages to human epithelial tumors, and that these tumor-associated macrophages release signals for proliferation and tumor growth of the epithelium. This Extra View article summarizes these recent findings with a focus on the role of eROS for promoting regeneration and inflammation-induced tumorigenesis.","PeriodicalId":12128,"journal":{"name":"Fly","volume":"11 1","pages":"46-52"},"PeriodicalIF":1.2,"publicationDate":"2017-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/19336934.2016.1222997","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34347642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 28

From the editors 来自编辑

IF 1.2 4区生物学

Fly Pub Date : 2017-01-02 DOI: 10.1080/19336934.2016.1276793

Blandine Lapercheis

引用次数: 0

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