Fly最新文献 - Book学术

Exploring the versatility of Drosophila melanogaster as a model organism in biomedical research: a comprehensive review. 探索黑腹果蝇作为模式生物在生物医学研究中的多功能性：综述。

IF 2.4 4区生物学

Fly Pub Date : 2025-12-01 Epub Date: 2024-12-25 DOI: 10.1080/19336934.2024.2420453

Ayomide Victor Atoki, Patrick Maduabuchi Aja, Tijjani Salihu Shinkafi, Erick Nyakundi Ondari, Adekunle Ismahil Adeniyi, Ilemobayo Victor Fasogbon, Reuben Samson Dangana, Umar Uthman Shehu, Akinpelumi Akin-Adewumi

{"title":"Exploring the versatility of Drosophila melanogaster as a model organism in biomedical research: a comprehensive review.","authors":"Ayomide Victor Atoki, Patrick Maduabuchi Aja, Tijjani Salihu Shinkafi, Erick Nyakundi Ondari, Adekunle Ismahil Adeniyi, Ilemobayo Victor Fasogbon, Reuben Samson Dangana, Umar Uthman Shehu, Akinpelumi Akin-Adewumi","doi":"10.1080/19336934.2024.2420453","DOIUrl":"https://doi.org/10.1080/19336934.2024.2420453","url":null,"abstract":"Drosophila melanogaster is a highly versatile model organism that has profoundly advanced our understanding of human diseases. With more than 60% of its genes having human homologs, Drosophila provides an invaluable system for modelling a wide range of pathologies, including neurodegenerative disorders, cancer, metabolic diseases, as well as cardiac and muscular conditions. This review highlights key developments in utilizing Drosophila for disease modelling, emphasizing the genetic tools that have transformed research in this field. Technologies such as the GAL4/UAS system, RNA interference (RNAi) and CRISPR-Cas9 have enabled precise genetic manipulation, with CRISPR-Cas9 allowing for the introduction of human disease mutations into orthologous Drosophila genes. These approaches have yielded critical insights into disease mechanisms, identified novel therapeutic targets and facilitated both drug screening and toxicological studies. Articles were selected based on their relevance, impact and contribution to the field, with a particular focus on studies offering innovative perspectives on disease mechanisms or therapeutic strategies. Our findings emphasize the central role of Drosophila in studying complex human diseases, underscoring its genetic similarities to humans and its effectiveness in modelling conditions such as Alzheimer's disease, Parkinson's disease and cancer. This review reaffirms Drosophila's critical role as a model organism, highlighting its potential to drive future research and therapeutic advancements.","PeriodicalId":12128,"journal":{"name":"Fly","volume":"19 1","pages":"2420453"},"PeriodicalIF":2.4,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142893246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Stage-specific modulation of Drosophila gene expression with muscle GAL4 promoters. 肌肉GAL4启动子对果蝇基因表达的阶段性调控。

IF 2.4 4区生物学

Fly Pub Date : 2025-12-01 Epub Date: 2025-01-07 DOI: 10.1080/19336934.2024.2447617

Ziwei Zhao, Erika R Geisbrecht

引用次数: 0

Disentangling how the brain is wired. 解开大脑是如何连接的。

IF 2.4 4区生物学

Fly Pub Date : 2025-12-01 Epub Date: 2024-12-31 DOI: 10.1080/19336934.2024.2440950

Simon G Sprecher

引用次数: 0

Sleep timing in flies from "adolescence" to adulthood. 从“青春期”到成年期果蝇的睡眠时间。

IF 2.4 4区生物学

Fly Pub Date : 2025-12-01 Epub Date: 2024-12-30 DOI: 10.1080/19336934.2024.2448022

Esther D Domenie, Paola Cusumano, Sara Montagnese, Rodolfo Costa

{"title":"Sleep timing in flies from \"adolescence\" to adulthood.","authors":"Esther D Domenie, Paola Cusumano, Sara Montagnese, Rodolfo Costa","doi":"10.1080/19336934.2024.2448022","DOIUrl":"https://doi.org/10.1080/19336934.2024.2448022","url":null,"abstract":"The aim of the present study was to assess sleep timing in Drosophila melanogaster at different ages, within the setting of an enforced schedule of varying light-dark stimuli, simulating light exposure variations between four typical office working days and one free day spent outside by a human, for a total of 30 days. Locomotor activity recording started when male flies were 3 days old. Flies exhibited a bimodal activity pattern, with a morning and an evening peak, and clear anticipation of the lights on and lights off transitions. From experimental day 10 (i.e. 12-day-old flies) onwards, a decrease in activity counts/increase in sleep amount were observed. On free days, a rise in activity counts and a reduction in sleep amount during the lights on interval was observed and was also present, albeit less obvious, on the subsequent working day during the lights off interval. A progressive delay in sleep onset was observed in the first days of the experiment, peaking on day 4 (i.e. 6-day-old flies), after which sleep onset timing gradually advanced. A delay in sleep offset was also observed for the first 13 days of the experiment, after which sleep offset stabilized. In conclusion, 'adolescent' flies exhibited changes in sleep timing that were reminiscent of those of human adolescents.","PeriodicalId":12128,"journal":{"name":"Fly","volume":"19 1","pages":"2448022"},"PeriodicalIF":2.4,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11702927/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142946810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

25 years of Drosophila "Sleep genes". 25年的果蝇“睡眠基因”。

IF 2.4 4区生物学

Fly Pub Date : 2025-12-01 Epub Date: 2025-05-06 DOI: 10.1080/19336934.2025.2502180

Orie Thomas Shafer

引用次数: 0

An updated proteomic analysis of Drosophila haemolymph after bacterial infection. 细菌感染后果蝇血淋巴的最新蛋白质组学分析。

IF 2.4 4区生物学

Fly Pub Date : 2025-12-01 Epub Date: 2025-04-13 DOI: 10.1080/19336934.2025.2485685

Samuel Rommelaere, Fanny Schüpfer, Florence Armand, Romain Hamelin, Bruno Lemaitre

引用次数: 0

Experimental assessment of 3D-printed traps and chemical attractants for the collection of wild Drosophila melanogaster. 野生黑腹果蝇采集3d打印陷阱和化学引诱剂的实验评估。

IF 2.4 4区生物学

Fly Pub Date : 2025-12-01 Epub Date: 2025-05-14 DOI: 10.1080/19336934.2025.2502184

Alexandra H Keene-Snickers, Tillie J Dunham, Mark D Stenglein

{"title":"Experimental assessment of 3D-printed traps and chemical attractants for the collection of wild Drosophila melanogaster.","authors":"Alexandra H Keene-Snickers, Tillie J Dunham, Mark D Stenglein","doi":"10.1080/19336934.2025.2502184","DOIUrl":"10.1080/19336934.2025.2502184","url":null,"abstract":"Drosophila melanogaster, the common fruit fly, has been instrumental to our understanding of evolution, genetics and disease. There are benefits to studying these flies in the wild, including assessment of their naturally occurring microbiota. To facilitate efforts to catch wild D. melanogaster, we designed two fly traps and evaluated several candidate attractants. The first trap utilized a stable food substrate that can be used to catch live flies to establish new lab colonies. The second trap was designed to be reusable and easy to ship to enable the collection of flies over time from diverse locations. We evaluated several chemical attractants derived from banana and from marula fruit, which is the proposed ancestral food host of D. melanogaster. We found that wild flies were preferentially attracted to banana-based odorants over marula-derived ones. Overall, these traps and attractants represent an inexpensive and simple option for the collection of wild D. melanogaster and related species for sampling or colony establishment.","PeriodicalId":12128,"journal":{"name":"Fly","volume":"19 1","pages":"2502184"},"PeriodicalIF":2.4,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12087654/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144076854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of adaptation to crowded larval environment on the evolution of sperm competitive ability in males of Drosophila melanogaster. 适应拥挤的幼虫环境对雄性黑腹果蝇精子竞争能力进化的影响。

IF 2.4 4区生物学

Fly Pub Date : 2025-12-01 Epub Date: 2024-12-18 DOI: 10.1080/19336934.2024.2437204

Rohit Kapila, Komal Maggu, Neetika Ahlawat, Nagaraj Guru Prasad

{"title":"Effects of adaptation to crowded larval environment on the evolution of sperm competitive ability in males of Drosophila melanogaster.","authors":"Rohit Kapila, Komal Maggu, Neetika Ahlawat, Nagaraj Guru Prasad","doi":"10.1080/19336934.2024.2437204","DOIUrl":"10.1080/19336934.2024.2437204","url":null,"abstract":"Two of the most important environmental factors that affect the sperm competitive ability in males are the availability of resources and the socio-sexual environment. Numerous studies have investigated the individual effects of these factors, but their combined effect on the evolution of sperm competitive ability remains untested. A crowded larval environment is unique because it simultaneously affects the fitness of the organism through both resource availability and the socio-sexual environment. In this study, we used a set of four laboratory populations of D. melanogaster, evolved under a crowded larval environment for more than 165 generations and their respective controls to investigate how the sperm competitive ability of the males is affected by a single generation of larval crowding versus evolution under a crowded larval environment for more than 165 generations. Our results show that larval crowding negatively affects the sperm defence ability of males evolved in a crowded larval environment, while it has no effect on the sperm defence ability of control males. Additionally, larval crowding negatively impacts the sperm offence ability in both control and evolved populations. Males from populations adapted to a crowded larval environment exhibit lower sperm offence ability at an older age compared to control populations.","PeriodicalId":12128,"journal":{"name":"Fly","volume":"19 1","pages":"2437204"},"PeriodicalIF":2.4,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11660399/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142853174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancing drug administration in Drosophila melanogaster: a method for using solid dispersions for improved solubility and bioavailability. 加强黑腹果蝇的给药：一种使用固体分散体提高溶解度和生物利用度的方法。

IF 2.4 4区生物学

Fly Pub Date : 2025-12-01 Epub Date: 2025-04-25 DOI: 10.1080/19336934.2025.2497565

Sunayn Cheku, Blase Rokusek, Mahesh Pattabiraman, Kimberly A Carlson

{"title":"Enhancing drug administration in Drosophila melanogaster: a method for using solid dispersions for improved solubility and bioavailability.","authors":"Sunayn Cheku, Blase Rokusek, Mahesh Pattabiraman, Kimberly A Carlson","doi":"10.1080/19336934.2025.2497565","DOIUrl":"https://doi.org/10.1080/19336934.2025.2497565","url":null,"abstract":"Drosophila melanogaster is a widely used model organism for diseases such as Parkinson's disease, Alzheimer's disease, obesity, and diabetes. However, compound administration-based toxicological and behavioural studies on Drosophila have been hindered by technical difficulties associated with inefficient administration of hydrophobic compounds. This study illustrates a general method to make and distribute PEG 8000-based solid dispersions for three hydrophobic compounds, distearoylglycerol (DSG) geldanamycin (GA) and RU486 to D. melanogaster. The solid dispersions were validated, in vitro, using nuclear magnetic resonance spectroscopy (NMR), to have a higher aqueous solubility. The study also describes three different methods to administer the solid dispersions: subcutaneous injections, mixing in solid food, and the capillary feeder assay (CAFE). We show that the presence of 1% DMSO decreases survival, whereas PEG does not have an adverse effect. Lastly, we showed that the prepared PEG-RU486 formulation showed signs of enhanced bioavailability when compared to RU486 dissolved in ethanol. The methodology described in the study provides an easy and effective means to administer hydrophobic compounds to D. melanogaster using subcutaneous injections, CAFE assay, or by mixing it with solid food.","PeriodicalId":12128,"journal":{"name":"Fly","volume":"19 1","pages":"2497565"},"PeriodicalIF":2.4,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12036485/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143988980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Open carry. 公开携带枪支。

IF 2.4 4区生物学

Fly Pub Date : 2025-12-01 Epub Date: 2025-05-21 DOI: 10.1080/19336934.2025.2503624

Howy Jacobs

引用次数: 0