果蝇的一种新型脂肪功能缺失突变体

IF 2.4 4区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
Fly Pub Date : 2024-12-01 Epub Date: 2024-05-13 DOI:10.1080/19336934.2024.2352938
Nicole A Losurdo, Adriana Bibo, Jacob Bedke, Nichole Link
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引用次数: 0

摘要

为了确定大脑生长所需的基因,我们在果蝇中采用了 RNAi 敲除反向遗传方法。其中一个潜在的候选基因是抗脂肪生成基因adipose(adp)。Adp 在负向调节果蝇脂肪体和哺乳动物脂肪组织的脂肪生成方面具有公认的作用。虽然脂肪是正常发育的关键,但在大脑发育过程中,adp 还没有被研究过。在这里,我们发现 RNAi 敲除神经干细胞和神经元中的 adp 会导致脑叶体积缩小,并试图用突变体苍蝇复制这一结果。根据浮力测定结果,我们生成了一种新型 adp 突变体,它是一种功能缺失突变体。我们发现,尽管体内脂肪含量总体上发生了变化,较大(>5 µm)脑脂滴的数量也有所减少,但突变体幼虫的脑叶体积没有变化。总之,我们的工作描述了一种新型的adp突变体,它可以在功能上替代长期存在的adp60突变体,并表明adp基因没有明显参与脑部生长。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
A novel adipose loss-of-function mutant in Drosophila.

To identify genes required for brain growth, we took an RNAi knockdown reverse genetic approach in Drosophila. One potential candidate isolated from this effort is the anti-lipogenic gene adipose (adp). Adp has an established role in the negative regulation of lipogenesis in the fat body of the fly and adipose tissue in mammals. While fat is key to proper development in general, adp has not been investigated during brain development. Here, we found that RNAi knockdown of adp in neuronal stem cells and neurons results in reduced brain lobe volume and sought to replicate this with a mutant fly. We generated a novel adp mutant that acts as a loss-of-function mutant based on buoyancy assay results. We found that despite a change in fat content in the body overall and a decrease in the number of larger (>5 µm) brain lipid droplets, there was no change in the brain lobe volume of mutant larvae. Overall, our work describes a novel adp mutant that can functionally replace the long-standing adp60 mutant and shows that the adp gene has no obvious involvement in brain growth.

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来源期刊
Fly
Fly 生物-生化与分子生物学
CiteScore
2.90
自引率
0.00%
发文量
17
审稿时长
>12 weeks
期刊介绍: Fly is the first international peer-reviewed journal to focus on Drosophila research. Fly covers a broad range of biological sub-disciplines, ranging from developmental biology and organogenesis to sensory neurobiology, circadian rhythm and learning and memory, to sex determination, evolutionary biology and speciation. We strive to become the “to go” resource for every researcher working with Drosophila by providing a forum where the specific interests of the Drosophila community can be discussed. With the advance of molecular technologies that enable researchers to manipulate genes and their functions in many other organisms, Fly is now also publishing papers that use other insect model systems used to investigate important biological questions. Fly offers a variety of papers, including Original Research Articles, Methods and Technical Advances, Brief Communications, Reviews and Meeting Reports. In addition, Fly also features two unconventional types of contributions, Counterpoints and Extra View articles. Counterpoints are opinion pieces that critically discuss controversial papers questioning current paradigms, whether justified or not. Extra View articles, which generally are solicited by Fly editors, provide authors of important forthcoming papers published elsewhere an opportunity to expand on their original findings and discuss the broader impact of their discovery. Extra View authors are strongly encouraged to complement their published observations with additional data not included in the original paper or acquired subsequently.
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