A direct-drive GFP reporter for studies of tracheal development in Drosophila.

IF 2.4 4区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Fly Pub Date : 2022-12-01 DOI:10.1080/19336934.2022.2030191

Geanette Lam, Katherine Beebe, Carl S Thummel

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Abstract

The Drosophila tracheal system consists of a widespread tubular network that provides respiratory functions for the animal. Its development, from ten pairs of placodes in the embryo to the final stereotypical branched structure in the adult, has been extensively studied by many labs as a model system for understanding tubular epithelial morphogenesis. Throughout these studies, a breathless (btl)-GAL4 driver has provided an invaluable tool to either mark tracheal cells during development or to manipulate gene expression in this tissue. A distinct shortcoming of this approach, however, is that btl-GAL4 cannot be used to specifically visualize tracheal cells in the presence of other GAL4 drivers or other UAS constructs, restricting its utility. Here we describe a direct-drive btl-nGFP reporter that can be used as a specific marker of tracheal cells throughout development in combination with any GAL4 driver and/or UAS construct. This reporter line should facilitate the use of Drosophila as a model system for studies of tracheal development and tubular morphogenesis.

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用于果蝇气管发育研究的直接驱动GFP报告器。

果蝇的气管系统由一个广泛的管状网络组成，为动物提供呼吸功能。它的发育，从胚胎的十对基板到成人的最终定型分支结构，已经被许多实验室广泛研究作为理解小管上皮形态发生的模型系统。在这些研究中，屏气(btl)-GAL4驱动因子为在气管细胞发育过程中标记或操纵该组织中的基因表达提供了宝贵的工具。然而，这种方法的一个明显缺点是，在其他GAL4驱动因子或其他UAS结构存在的情况下，btl-GAL4不能用于特异性地观察气管细胞，限制了它的实用性。在这里，我们描述了一种直接驱动的btl-nGFP报告基因，它可以与任何GAL4驱动基因和/或UAS结构相结合，作为气管细胞在整个发育过程中的特定标记。这条报告细胞系有助于将果蝇作为研究气管发育和小管形态发生的模型系统。

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来源期刊

Fly 生物-生化与分子生物学

CiteScore

2.90

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Fly is the first international peer-reviewed journal to focus on Drosophila research. Fly covers a broad range of biological sub-disciplines, ranging from developmental biology and organogenesis to sensory neurobiology, circadian rhythm and learning and memory, to sex determination, evolutionary biology and speciation. We strive to become the “to go” resource for every researcher working with Drosophila by providing a forum where the specific interests of the Drosophila community can be discussed. With the advance of molecular technologies that enable researchers to manipulate genes and their functions in many other organisms, Fly is now also publishing papers that use other insect model systems used to investigate important biological questions. Fly offers a variety of papers, including Original Research Articles, Methods and Technical Advances, Brief Communications, Reviews and Meeting Reports. In addition, Fly also features two unconventional types of contributions, Counterpoints and Extra View articles. Counterpoints are opinion pieces that critically discuss controversial papers questioning current paradigms, whether justified or not. Extra View articles, which generally are solicited by Fly editors, provide authors of important forthcoming papers published elsewhere an opportunity to expand on their original findings and discuss the broader impact of their discovery. Extra View authors are strongly encouraged to complement their published observations with additional data not included in the original paper or acquired subsequently.