IF 1.2 4区生物学

Fly Pub Date : 2022-12-01 DOI: 10.1080/19336934.2021.2016327

Anneli Hoikkala, Noora Poikela

{"title":"Adaptation and ecological speciation in seasonally varying environments at high latitudes: Drosophila virilis group.","authors":"Anneli Hoikkala, Noora Poikela","doi":"10.1080/19336934.2021.2016327","DOIUrl":"https://doi.org/10.1080/19336934.2021.2016327","url":null,"abstract":"Living in high latitudes and altitudes sets specific requirements on species' ability to forecast seasonal changes and to respond to them in an appropriate way. Adaptation into diverse environmental conditions can also lead to ecological speciation through habitat isolation or by inducing changes in traits that influence assortative mating. In this review, we explain how the unique time-measuring systems of Drosophila virilis group species have enabled the species to occupy high latitudes and how the traits involved in species reproduction and survival exhibit strong linkage with latitudinally varying photoperiodic and climatic conditions. We also describe variation in reproductive barriers between the populations of two species with overlapping distributions and show how local adaptation and the reinforcement of prezygotic barriers have created partial reproductive isolation between conspecific populations. Finally, we consider the role of species-specific chromosomal inversions and the X chromosome in the development of reproductive barriers between diverging lineages.","PeriodicalId":12128,"journal":{"name":"Fly","volume":" ","pages":"85-104"},"PeriodicalIF":1.2,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8786326/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39846841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Ancestral dietary change alters the development of Drosophila larvae through MAPK signalling. 祖先的饮食改变通过MAPK信号改变果蝇幼虫的发育。

IF 1.2 4区生物学

Fly Pub Date : 2022-12-01 DOI: 10.1080/19336934.2022.2088032

Samuel G Towarnicki, Neil A Youngson, Susan M Corley, Jus C St John, Richard G Melvin, Nigel Turner, Margaret J Morris, J William O Ballard

{"title":"Ancestral dietary change alters the development of Drosophila larvae through MAPK signalling.","authors":"Samuel G Towarnicki, Neil A Youngson, Susan M Corley, Jus C St John, Richard G Melvin, Nigel Turner, Margaret J Morris, J William O Ballard","doi":"10.1080/19336934.2022.2088032","DOIUrl":"https://doi.org/10.1080/19336934.2022.2088032","url":null,"abstract":"Studies in a broad range of animal species have revealed phenotypes that are caused by ancestral life experiences, including stress and diet. Ancestral dietary macronutrient composition and quantity (over- and under-nutrition) have been shown to alter descendent growth, metabolism and behaviour. Molecules have been identified in gametes that are changed by ancestral diet and are required for transgenerational effects. However, there is less understanding of the developmental pathways altered by inherited molecules during the period between fertilization and adulthood. To investigate this non-genetic inheritance, we exposed great grand-parental and grand-parental generations to defined protein to carbohydrate (P:C) dietary ratios. Descendent developmental timing was consistently faster in the period between the embryonic and pupal stages when ancestors had a higher P:C ratio diet. Transcriptional analysis revealed extensive and long-lasting changes to the MAPK signalling pathway, which controls growth rate through the regulation of ribosomal RNA transcription. Pharmacological inhibition of both MAPK and rRNA pathways recapitulated the ancestral diet-induced developmental changes. This work provides insight into non-genetic inheritance between fertilization and adulthood.","PeriodicalId":12128,"journal":{"name":"Fly","volume":"16 1","pages":"299-311"},"PeriodicalIF":1.2,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9354765/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10528622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

A robust method to isolate Drosophila fat body nuclei for transcriptomic analysis. 一种分离果蝇脂肪体核进行转录组学分析的可靠方法。

IF 1.2 4区生物学

Fly Pub Date : 2022-12-01 DOI: 10.1080/19336934.2021.1978776

Vanika Gupta, Brian P Lazzaro

{"title":"A robust method to isolate Drosophila fat body nuclei for transcriptomic analysis.","authors":"Vanika Gupta, Brian P Lazzaro","doi":"10.1080/19336934.2021.1978776","DOIUrl":"https://doi.org/10.1080/19336934.2021.1978776","url":null,"abstract":"Gene expression profiles are typically described at the level of the tissue or, often in Drosophila, at the level of the whole organism. Collapsing the gene expression of entire tissues into single measures averages over potentially important heterogeneity among the cells that make up that tissue. The advent of single-cell RNA-sequencing technology (sc-RNAseq) allows transcriptomic evaluation of the individual cells that make up a tissue. However, sc-RNAseq requires a high-quality suspension of viable cells or nuclei, and cell dissociation methods that yield healthy cells and nuclei are still lacking for many important tissues. The insect fat body is a polyfunctional tissue responsible for diverse physiological processes and therefore is an important target for sc-RNAseq. The Drosophila adult fat body consists of fragile cells that are difficult to dissociate while maintaining cell viability. As an alternative, we developed a method to isolate single fat body nuclei for RNA-seq. Our isolation method is largely free of mitochondrial contamination and yields higher capture of transcripts per nucleus compared to other nuclei preparation methods. Our method works well for single-cell nuclei sequencing and can potentially be implemented for bulk RNA-seq.","PeriodicalId":12128,"journal":{"name":"Fly","volume":" ","pages":"62-67"},"PeriodicalIF":1.2,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8500699/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39488730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

HaloTag-based reporters for sparse labeling and cell tracking. 基于halotag的报告器，用于稀疏标记和细胞跟踪。

IF 1.2 4区生物学

Fly Pub Date : 2022-12-01 DOI: 10.1080/19336934.2022.2142460

Lydie Couturier, Juan Luna, Khalil Mazouni, Claire Mestdagh, Minh-Son Phan, Francis Corson, Francois Schweisguth

{"title":"HaloTag-based reporters for sparse labeling and cell tracking.","authors":"Lydie Couturier, Juan Luna, Khalil Mazouni, Claire Mestdagh, Minh-Son Phan, Francis Corson, Francois Schweisguth","doi":"10.1080/19336934.2022.2142460","DOIUrl":"https://doi.org/10.1080/19336934.2022.2142460","url":null,"abstract":"Multiscale analysis of morphogenesis requires to follow and measure in real-time the in vivo behaviour of large numbers of individual cells over long period of time. Despite recent progress, the large-scale automated tracking of cells in developing embryos and tissues remains a challenge. Here we describe a genetic tool for the random and sparse labelling of individual cells in developing Drosophila tissues. This tool is based on the conditional expression of a nuclear HaloTag protein that can be fluorescently labelled upon the irreversible binding of a cell permeable synthetic ligand. While the slow maturation of genetically encoded fluorescent renders the tracking of individual cells difficult in rapidly dividing tissues, nuclear HaloTag proteins allowed for rapid labelling of individual cells in cultured imaginal discs. To study cell shape changes, we also produced an HaloTag version of the actin-bound protein LifeAct. Since sparse labelling facilitates cell tracking, nuclear HaloTag reporters will be useful for the single-cell analysis of fate dynamics in Drosophila tissues cultured ex vivo.","PeriodicalId":12128,"journal":{"name":"Fly","volume":"16 1","pages":"360-366"},"PeriodicalIF":1.2,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9635558/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10474465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Standardized terminology and visual atlas of the external morphology and terminalia for the genus Scaptomyza (Diptera: Drosophilidae). 水虻属外形和终末物的标准化术语和视觉图谱(双翅目:果蝇科)。

IF 1.2 4区生物学

Fly Pub Date : 2022-12-01 DOI: 10.1080/19336934.2021.1969220

Augusto Santos Rampasso, Patrick Michael O'Grady

{"title":"Standardized terminology and visual atlas of the external morphology and terminalia for the genus Scaptomyza (Diptera: Drosophilidae).","authors":"Augusto Santos Rampasso, Patrick Michael O'Grady","doi":"10.1080/19336934.2021.1969220","DOIUrl":"https://doi.org/10.1080/19336934.2021.1969220","url":null,"abstract":"ABSTRACT The genus Scaptomyza is one of the two Drosophilidae genera with Hawaiian endemic species. This genus is an excellent model for biogeographic studies since it is distributed throughout the majority of continents, including continental islands, the Hawaiian Islands, and many other remote oceanic islands. This genus currently comprises 273 described species, 148 of which are endemic to the Hawaiian Islands. However, most descriptions were published before efforts to standardizing the morphological terminology across the Diptera were made in the 1980’s. Since research groups developed their own set of terminologies independently, without considering homologies, multiple terms have been used to refer to the same characters. This is especially true for the male terminalia, which have remarkable modifications within the family Drosophilidae. We reviewed the Scaptomyza literature, in addition to other studies across the Drosophilidae and Diptera, compiled the English synonyms, and provided a visual atlas of each body part, indicating how to recognize the morphological characters. The goal of the present study is to facilitate species identification and propose preferred terms to be adopted for future Scaptomyza descriptions.","PeriodicalId":12128,"journal":{"name":"Fly","volume":" ","pages":"37-61"},"PeriodicalIF":1.2,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8525988/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39513254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

A screen of small molecule and genetic modulators of life span in female Drosophila identifies etomoxir, RH5849 and unanticipated temperature effects. 对雌果蝇寿命的小分子和基因调节剂进行筛选，发现了依托莫西尔、RH5849 和意想不到的温度效应。

IF 2.4 4区生物学

Fly Pub Date : 2022-12-01 DOI: 10.1080/19336934.2022.2149209

Gary N Landis, Sebastian Ko, Oscar Peng, Brett Bognar, Michael Khmelkov, Hans S Bell, John Tower

{"title":"A screen of small molecule and genetic modulators of life span in female Drosophila identifies etomoxir, RH5849 and unanticipated temperature effects.","authors":"Gary N Landis, Sebastian Ko, Oscar Peng, Brett Bognar, Michael Khmelkov, Hans S Bell, John Tower","doi":"10.1080/19336934.2022.2149209","DOIUrl":"10.1080/19336934.2022.2149209","url":null,"abstract":"Mifepristone increases life span in female Drosophila melanogaster, and its molecular target(s) remain unclear. Here small molecule and genetic interventions were tested for ability to mimic mifepristone, or to decrease life span in a way that can be rescued by mifepristone. Etomoxir inhibits lipid metabolism, and significantly increased life span in virgin and mated females, but not males, at 50 µM concentration. Pioglitazone is reported to activate both mammalian PPARγ and its Drosophila homolog Eip75B. Pioglitazone produced minor and inconsistent benefits for female Drosophila life span, and only at the lowest concentrations tested. Ecdysone is a Drosophila steroid hormone reported to regulate responses to mating, and RH5849 is a potent mimic of ecdysone. RH5849 reduced virgin female life span, and this was partly rescued by mifepristone. Mifepristone did not compete with RH5849 for activation of an ecdysone receptor (EcR)-responsive transgenic reporter, indicating that the relevant target for mifepristone is not EcR. The conditional GAL4/GAL80ts system was used in attempt to test the effect of an Eip75B RNAi construct on female life span. However, the 29°C temperature used for induction reduced or eliminated mating-induced midgut hypertrophy, the negative life span effects of mating, and the positive life span effects of mifepristone. Even when applied after mating was complete, a shift to 29°C temperature reduced mating-induced midgut hypertrophy by half, and the life span effects of mating by 4.8-fold. Taken together, these results identify promising small molecules for further analysis, and inform the design of experiments involving the GAL4/GAL80ts system.","PeriodicalId":12128,"journal":{"name":"Fly","volume":"16 1","pages":"397-413"},"PeriodicalIF":2.4,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9683069/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10474700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The role of micro RNAs (miRNAs) in the regulation of Drosophila melanogaster's innate immunity. 微rna (miRNAs)在果蝇先天免疫调节中的作用。

IF 1.2 4区生物学

Fly Pub Date : 2022-12-01 DOI: 10.1080/19336934.2022.2149204

Max Yang Lu, Stanislava Chtarbanova

引用次数: 2

Interorgan communication through peripherally derived peptide hormones in Drosophila. 果蝇通过外周肽类激素进行器官间交流

IF 2.4 4区生物学

Fly Pub Date : 2022-12-01 DOI: 10.1080/19336934.2022.2061834

Naoki Okamoto, Akira Watanabe

引用次数: 0

Dynamic movement and turnover of extracellular matrices during tissue development and maintenance. 细胞外基质在组织发育和维持过程中的动态运动和周转。

IF 2.4 4区生物学

Fly Pub Date : 2022-12-01 DOI: 10.1080/19336934.2022.2076539

Yutaka Matsubayashi

引用次数: 0

Notch Signalling Under Maternal-to-Zygotic Transition. 母系转合子过程中的Notch信号传导。

IF 1.2 4区生物学

Fly Pub Date : 2022-12-01 DOI: 10.1080/19336934.2022.2139981

Tomoko Yamakawa, Elzava Yuslimatin Mujizah, Kenji Matsuno

{"title":"Notch Signalling Under Maternal-to-Zygotic Transition.","authors":"Tomoko Yamakawa, Elzava Yuslimatin Mujizah, Kenji Matsuno","doi":"10.1080/19336934.2022.2139981","DOIUrl":"https://doi.org/10.1080/19336934.2022.2139981","url":null,"abstract":"The development of all animal embryos is initially directed by the gene products supplied by their mothers. With the progression of embryogenesis, the embryo's genome is activated to command subsequent developments. This transition, which has been studied in many model animals, is referred to as the Maternal-to-Zygotic Transition (MZT). In many organisms, including flies, nematodes, and sea urchins, genes involved in Notch signaling are extensively influenced by the MZT. This signaling pathway is highly conserved across metazoans; moreover, it regulates various developmental processes. Notch signaling defects are commonly associated with various human diseases. The maternal contribution of its factors was first discovered in flies. Subsequently, several genes were identified from mutant embryos with a phenotype similar to Notch mutants only upon the removal of the maternal contributions. Studies on these maternal genes have revealed various novel steps in the cascade of Notch signal transduction. Among these genes, pecanex and almondex have been functionally characterized in recent studies. Therefore, in this review, we will focus on the roles of these two maternal genes in Notch signaling and discuss future research directions on its maternal function.","PeriodicalId":12128,"journal":{"name":"Fly","volume":"16 1","pages":"347-359"},"PeriodicalIF":1.2,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9645253/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10472596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Fly最新文献