Fly最新文献_第4页

Cell-cell interactions that drive tumorigenesis in Drosophila. 驱动果蝇肿瘤发生的细胞-细胞相互作用。

IF 1.2 4区生物学

Fly Pub Date : 2022-12-01 DOI: 10.1080/19336934.2022.2148828

Masato Enomoto, Tatsushi Igaki

引用次数: 2

Cell mechanics and cell-cell recognition controls by Toll-like receptors in tissue morphogenesis and homeostasis Toll样受体在组织形态发生和稳态中的细胞力学和细胞-细胞识别控制

IF 1.2 4区生物学

Fly Pub Date : 2022-05-17 DOI: 10.1080/19336934.2022.2074783

Daiki Umetsu

引用次数: 5

Cutting edge technologies expose the temporal regulation of neurogenesis in the Drosophila nervous system 尖端技术揭示了果蝇神经系统神经发生的时间调节

IF 1.2 4区生物学

Fly Pub Date : 2022-05-13 DOI: 10.1080/19336934.2022.2073158

Makoto Sato, Takumi Suzuki

{"title":"Cutting edge technologies expose the temporal regulation of neurogenesis in the Drosophila nervous system","authors":"Makoto Sato, Takumi Suzuki","doi":"10.1080/19336934.2022.2073158","DOIUrl":"https://doi.org/10.1080/19336934.2022.2073158","url":null,"abstract":"ABSTRACT During the development of the central nervous system (CNS), extremely large numbers of neurons are produced in a regular fashion to form precise neural circuits. During this process, neural progenitor cells produce different neurons over time due to their intrinsic gene regulatory mechanisms as well as extrinsic mechanisms. The Drosophila CNS has played an important role in elucidating the temporal mechanisms that control neurogenesis over time. It has been shown that a series of temporal transcription factors are sequentially expressed in neural progenitor cells and regulate the temporal specification of neurons in the embryonic CNS. Additionally, similar mechanisms are found in the developing optic lobe and central brain in the larval CNS. However, it is difficult to elucidate the function of numerous molecules in many different cell types solely by molecular genetic approaches. Recently, omics analysis using single-cell RNA-seq and other methods has been used to study the Drosophila nervous system on a large scale and is making a significant contribution to the understanding of the temporal mechanisms of neurogenesis. In this article, recent findings on the temporal patterning of neurogenesis and the contributions of cutting-edge technologies will be reviewed.","PeriodicalId":12128,"journal":{"name":"Fly","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2022-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49020058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Biology and ecology of the Oriental flower-breeding Drosophila elegans and related species 东方繁花果蝇及其近缘种的生物学与生态学

IF 1.2 4区生物学

Fly Pub Date : 2022-05-01 DOI: 10.1080/19336934.2022.2066953

Yuki Ishikawa, M. Kimura, M. Toda

引用次数: 4

The developing wing crossvein of Drosophila melanogaster: a fascinating model for signaling and morphogenesis 黑腹果蝇正在发育的翅膀横静脉：一个迷人的信号传导和形态发生模型

IF 1.2 4区生物学

Fly Pub Date : 2022-03-18 DOI: 10.1080/19336934.2022.2040316

Hanna Antson, Tambet Tõnissoo, O. Shimmi

引用次数: 3

Structure-function analysis of Cdc25Twine degradation at the Drosophila maternal-to-zygotic transition Cdc25Twine在果蝇母体向合子转化过程中降解的结构-功能分析

IF 1.2 4区生物学

Fly Pub Date : 2022-02-28 DOI: 10.1080/19336934.2022.2043095

P. Ferree, Maggie Xing, Jenny Zhang, Stefano Di Talia

引用次数: 0

Flying remote. 远程飞行。

IF 1.2 4区生物学

Fly Pub Date : 2021-12-01 DOI: 10.1080/19336934.2021.1884033

Howy Jacobs

引用次数: 0

In vivo assay and modelling of protein and mitochondrial turnover during aging. 衰老过程中蛋白质和线粒体周转的活体检测和建模。

IF 2.4 4区生物学

Fly Pub Date : 2021-12-01 DOI: 10.1080/19336934.2021.1911286

Hans S Bell, John Tower

{"title":"In vivo assay and modelling of protein and mitochondrial turnover during aging.","authors":"Hans S Bell, John Tower","doi":"10.1080/19336934.2021.1911286","DOIUrl":"10.1080/19336934.2021.1911286","url":null,"abstract":"To maintain homoeostasis, cells must degrade damaged or misfolded proteins and synthesize functional replacements. Maintaining a balance between these processes, known as protein turnover, is necessary for stress response and cellular adaptation to a changing environment. Damaged mitochondria must also be removed and replaced. Changes in protein and mitochondrial turnover are associated with aging and neurodegenerative disease, making it important to understand how these processes occur and are regulated in cells. To achieve this, reliable assays of turnover must be developed. Several methods exist, including pulse-labelling with radioactive or stable isotopes and strategies making use of fluorescent proteins, each with their own advantages and limitations. Both cell culture and live animals have been used for these studies, in systems ranging from yeast to mammals. In vivo assays are especially useful for connecting turnover to aging and disease. With its short life cycle, suitability for fluorescent imaging, and availability of genetic tools, Drosophila melanogaster is particularly well suited for this kind of analysis.","PeriodicalId":12128,"journal":{"name":"Fly","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8143256/pdf/KFLY_15_1911286.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38993629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterizing a gene expression toolkit for eye- and photoreceptor-specific expression in Drosophila. 鉴定果蝇眼部和感光器特异性基因表达工具包。

IF 1.2 4区生物学

Fly Pub Date : 2021-12-01 DOI: 10.1080/19336934.2021.1915683

Spencer E Escobedo, Aashka Shah, Alyssa N Easton, Hana Hall, Vikki M Weake

{"title":"Characterizing a gene expression toolkit for eye- and photoreceptor-specific expression in Drosophila.","authors":"Spencer E Escobedo, Aashka Shah, Alyssa N Easton, Hana Hall, Vikki M Weake","doi":"10.1080/19336934.2021.1915683","DOIUrl":"10.1080/19336934.2021.1915683","url":null,"abstract":"Binary expression systems are a powerful tool for tissue- and cell-specific research. Many of the currently available Drosophila eye-specific drivers have not been systematically characterized for their expression level and cell-type specificity in the adult eye or during development. Here, we used a luciferase reporter to measure expression levels of different drivers in the adult Drosophila eye, and characterized the cell type-specificity of each driver using a fluorescent reporter in live 10-day-old adult males. We also further characterized the expression pattern of these drivers in various developmental stages. We compared several Gal4 drivers from the Bloomington Drosophila Stock Center (BDSC) including GMR-Gal4, longGMR-Gal4 and Rh1-Gal4 with newly developed Gal4 and QF2 drivers that are specific to different cell types in the adult eye. In addition, we generated drug-inducible Rh1-GSGal4 lines and compared their induced expression with an available GMR-GSGal4 line. Although both lines had significant induction of gene expression measured by luciferase activity, Rh1-GSGal4 was expressed at levels below the detection of the fluorescent reporter by confocal microscopy, while GMR-GSGal4 showed substantial reporter expression in the absence of drug by microscopy. Overall, our study systematically characterizes and compares a large toolkit of eye- and photoreceptor-specific drivers, while also uncovering some of the limitations of currently available expression systems in the adult eye.","PeriodicalId":12128,"journal":{"name":"Fly","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/19336934.2021.1915683","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38908488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Exploring Excitotoxicity and Regulation of a Constitutively Active TRP Ca²⁺ Channel in Drosophila. 探索果蝇组成活性TRP Ca2+通道的兴奋毒性和调控。

IF 1.2 4区生物学

Fly Pub Date : 2021-12-01 Epub Date: 2020-12-01 DOI: 10.1080/19336934.2020.1851586

Bih-Hwa Shieh, Lucinda Nuzum, Inga Kristaponyte

{"title":"Exploring Excitotoxicity and Regulation of a Constitutively Active TRP Ca2+ Channel in Drosophila.","authors":"Bih-Hwa Shieh, Lucinda Nuzum, Inga Kristaponyte","doi":"10.1080/19336934.2020.1851586","DOIUrl":"https://doi.org/10.1080/19336934.2020.1851586","url":null,"abstract":"Unregulated Ca2+ influx affects intracellular Ca2+ homoeostasis, which may lead to neuronal death. In Drosophila, following the activation of rhodopsin the TRP Ca2+ channel is open to mediate the light-dependent depolarization. A constitutively active TRP channel triggers the degeneration of TrpP365 /+ photoreceptors. To explore retinal degeneration, we employed a multidisciplinary approach including live imaging using GFP tagged actin and arrestin 2. Importantly, we demonstrate that the major rhodopsin (Rh1) was greatly reduced before the onset of rhabdomere degeneration; a great reduction of Rh1 affects the maintenance of rhabdomere leading to degeneration of photoreceptors. TrpP365 /+ also led to the up-regulation of CaMKII, which is beneficial as suppression of CaMKII accelerated retinal degeneration. We explored the regulation of TRP by investigating the genetic interaction between TrpP365 /+ and mutants affecting the turnover of diacylglycerol (DAG). We show a loss of phospholipase C in norpAP24 exhibited a great reduction of the DAG content delayed degeneration of TrpP365 /+ photoreceptors. In contrast, knockdown or mutations in DAG lipase (InaE) that is accompanied by slightly reduced levels of most DAG but an increased level of DAG 34:1, exacerbated retinal degeneration of TrpP365 /+. Together, our findings support the notion that DAG plays a role in regulating TRP. Interestingly, DAG lipase is likely required during photoreceptor development as TrpP365 /+; inaEN125 double mutants contained severely degenerated rhabdomeres.","PeriodicalId":12128,"journal":{"name":"Fly","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/19336934.2020.1851586","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38611004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3