Ancestral dietary change alters the development of Drosophila larvae through MAPK signalling.

IF 2.4 4区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
Fly Pub Date : 2022-12-01 DOI:10.1080/19336934.2022.2088032
Samuel G Towarnicki, Neil A Youngson, Susan M Corley, Jus C St John, Richard G Melvin, Nigel Turner, Margaret J Morris, J William O Ballard
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引用次数: 2

Abstract

Studies in a broad range of animal species have revealed phenotypes that are caused by ancestral life experiences, including stress and diet. Ancestral dietary macronutrient composition and quantity (over- and under-nutrition) have been shown to alter descendent growth, metabolism and behaviour. Molecules have been identified in gametes that are changed by ancestral diet and are required for transgenerational effects. However, there is less understanding of the developmental pathways altered by inherited molecules during the period between fertilization and adulthood. To investigate this non-genetic inheritance, we exposed great grand-parental and grand-parental generations to defined protein to carbohydrate (P:C) dietary ratios. Descendent developmental timing was consistently faster in the period between the embryonic and pupal stages when ancestors had a higher P:C ratio diet. Transcriptional analysis revealed extensive and long-lasting changes to the MAPK signalling pathway, which controls growth rate through the regulation of ribosomal RNA transcription. Pharmacological inhibition of both MAPK and rRNA pathways recapitulated the ancestral diet-induced developmental changes. This work provides insight into non-genetic inheritance between fertilization and adulthood.

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祖先的饮食改变通过MAPK信号改变果蝇幼虫的发育。
对多种动物物种的研究揭示了由祖先生活经历(包括压力和饮食)引起的表型。祖先的饮食宏量营养素组成和数量(营养过剩和营养不足)已被证明会改变后代的生长、代谢和行为。配子中的分子已经被鉴定出来,这些分子被祖先的饮食改变,并且需要跨代影响。然而,在受精和成年之间,遗传分子改变发育途径的理解较少。为了研究这种非基因遗传,我们将曾祖父母和曾祖父母暴露在确定的蛋白质与碳水化合物(P:C)膳食比例中。当祖先饮食中磷碳比较高时,在胚胎期和蛹期之间,后代的发育时间一直较快。转录分析揭示了MAPK信号通路广泛而持久的变化,该信号通路通过调节核糖体RNA转录来控制生长速度。MAPK和rRNA途径的药理抑制再现了祖先饮食诱导的发育变化。这项工作为受精和成年之间的非基因遗传提供了洞见。
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来源期刊
Fly
Fly 生物-生化与分子生物学
CiteScore
2.90
自引率
0.00%
发文量
17
审稿时长
>12 weeks
期刊介绍: Fly is the first international peer-reviewed journal to focus on Drosophila research. Fly covers a broad range of biological sub-disciplines, ranging from developmental biology and organogenesis to sensory neurobiology, circadian rhythm and learning and memory, to sex determination, evolutionary biology and speciation. We strive to become the “to go” resource for every researcher working with Drosophila by providing a forum where the specific interests of the Drosophila community can be discussed. With the advance of molecular technologies that enable researchers to manipulate genes and their functions in many other organisms, Fly is now also publishing papers that use other insect model systems used to investigate important biological questions. Fly offers a variety of papers, including Original Research Articles, Methods and Technical Advances, Brief Communications, Reviews and Meeting Reports. In addition, Fly also features two unconventional types of contributions, Counterpoints and Extra View articles. Counterpoints are opinion pieces that critically discuss controversial papers questioning current paradigms, whether justified or not. Extra View articles, which generally are solicited by Fly editors, provide authors of important forthcoming papers published elsewhere an opportunity to expand on their original findings and discuss the broader impact of their discovery. Extra View authors are strongly encouraged to complement their published observations with additional data not included in the original paper or acquired subsequently.
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