Experimental parasitology最新文献

{"title":"Enhancing chlamydospore production in Duddingtonia flagrans on solid substrate: The impact of mannitol and varied cultivation conditions","authors":"M. Junco ,&nbsp;L.E. Iglesias ,&nbsp;S. Zegbi ,&nbsp;M.F. Sagués ,&nbsp;I. Guerrero ,&nbsp;G. Bernat ,&nbsp;M.E. Fuentes ,&nbsp;E. Riva ,&nbsp;A.S. Fernández ,&nbsp;C.A. Saumell","doi":"10.1016/j.exppara.2024.108725","DOIUrl":"10.1016/j.exppara.2024.108725","url":null,"abstract":"<div><p><em>Duddingtonia flagrans</em> is a nematophagous fungus which has shown promising results as a non-chemical parasitic control tool. The fungus disrupts the parasite's life cycle by trapping larvae in the environment through the networks generated from chlamydospores, thus preventing the reinfection of animals. One barrier to the development of a commercial product using this tool is the need to increase chlamydospore production in the laboratory for its administration to livestock. The purpose of this study was to evaluate the addition of mannitol to an enriched culture medium and the effect of adverse cultivation conditions on chlamydospore production. <em>D. flagrans</em> was cultivated on Petri dishes with corn agar for 4 weeks at 27 °C and 70% relative humidity (RH). Four groups were then formed, all with Sabouraud agar as a base, to which different growth inducers were added: GSA (glucose Sabouraud agar), GSA-MI (glucose Sabouraud agar + meso inositol), GSA-E (enriched glucose Sabouraud agar), and AE-M (enriched agar + mannitol). After 4 weeks, chlamydospores were recovered by washing the surface of each plate with distilled water and then quantified. The medium that yielded the highest amount of chlamydospores was subjected to different cultivation conditions: NC (normal conditions): 70% RH and 27 °C, AC (adverse conditions) 1: 20% RH and 40 °C, CA2: 60% RH and 27 °C, and CA3: 55% RH and 24 °C. It was determined that mannitol increases chlamydospore production (65x10⁶ chlamydospores/plate), and when reducing humidity by 10% under cultivation conditions it resulted in an approximately 10% increase in chlamydospore production compared to the control group. These results suggest that the addition of polyols, as well as its cultivation under certain environmental conditions, can improve chlamydospore production on a laboratory scale.</p></div>","PeriodicalId":12117,"journal":{"name":"Experimental parasitology","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2024-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140065023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potent hydroxamate-derived compounds arrest endodyogeny of Toxoplasma gondii tachyzoites","authors":"Carlla Assis Araujo-Silva ,&nbsp;Katharina Vögerl ,&nbsp;Ferdinand Breu ,&nbsp;Manfred Jung ,&nbsp;Andreia Luiza Oliveira Costa ,&nbsp;Wanderley De Souza ,&nbsp;Franz Bracher ,&nbsp;Erica S. Martins-Duarte ,&nbsp;Rossiane C. Vommaro","doi":"10.1016/j.exppara.2024.108727","DOIUrl":"10.1016/j.exppara.2024.108727","url":null,"abstract":"<div><p>Toxoplasmosis is a zoonosis that is a worldwide health problem, commonly affecting fetal development and immunodeficient patients. Treatment is carried out with a combination of pyrimethamine and sulfadiazine, which can cause cytopenia and intolerance and does not lead to a parasitological cure of the infection. Lysine deacetylases (KDACs), which remove an acetyl group from lysine residues in histone and non-histone proteins are found in the <em>Toxoplasma gondii</em> genome. Previous work showed the hydroxamate-type KDAC inhibitors Tubastatin A (TST) and Vorinostat (Suberoylanilide Hydroxamic Acid, SAHA) were effective against <em>T. gondii</em>. In the present study, the effects of three hydroxamates (<strong>KV-24</strong>, <strong>KV-30</strong>, <strong>KV-46</strong>), which were originally designed to inhibit human KDAC6, showed different effects against <em>T. gondii</em>. These compounds contain a heterocyclic cap group and a benzyl linker bearing the hydroxamic acid group in <em>para</em>-position. All compounds showed selective activity against <em>T. gondii</em> proliferation, inhibiting tachyzoite proliferation with IC₅₀ values in a nanomolar range after 48h treatment. Microscopy analyses showed that after treatment, tachyzoites presented mislocalization of the apicoplast, disorganization of the inner membrane complex, and arrest in the completion of new daughter cells. The number of dividing cells with incomplete endodyogeny increased significantly after treatment, indicating the compounds can interfere in the late steps of cell division. The results obtained in this work that these new hydroxamates should be considered for future <em>in vivo</em> tests and the development of new compounds for treating toxoplasmosis.</p></div>","PeriodicalId":12117,"journal":{"name":"Experimental parasitology","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140021189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of annexin B18 from Echinococcus granulosus sensu lato on mouse macrophages","authors":"Yanxin Chen ,&nbsp;Ruiqi Hua ,&nbsp;Guoqing Shao ,&nbsp;Xiaowei Zhu ,&nbsp;Wei Hou ,&nbsp;Shengqiong Li ,&nbsp;Aiguo Yang ,&nbsp;Guangyou Yang","doi":"10.1016/j.exppara.2024.108723","DOIUrl":"10.1016/j.exppara.2024.108723","url":null,"abstract":"<div><p>Cystic echinococcosis (CE) is a zoonotic disease, caused by <em>Echinococcus granulosus</em> sensu lato (<em>E. granulosus</em> s. l.), which posed significant public health concern globally. <em>E. granulosus</em> s. l. annexin B18 (<em>Eg</em>ANXB18) acts as a secretory protein, exerting a crucial influence in mediating host-parasite interactions. Recombinant annexin B18 (r<em>Eg</em>ANXB18) was expressed by <em>Escherichia coli</em> and the immunoreactivity was assessed by western blotting. The binding affinity between r<em>Eg</em>ANXB18 and total protein of RAW264.7 cells was assessed by ELISA. The impact of r<em>Eg</em>ANXB18 on the metabolic activity of RAW264.7 cells was assayed by Cell Counting Kit-8 assay. The mRNA levels of polarization markers (inducible nitrous oxide synthase (iNOS) and arginase 1 (Arg1)) and key cellular factors (IL-1β，IL-6，IL-10 and TNFα) were evaluated by qRT-PCR. r<em>Eg</em>ANXB18 was successfully expressed and recognized by <em>E. granulosus</em> s.l. infected canine sera, as well as could bind to the total protein of RAW264.7 cells. Additionally, r<em>Eg</em>ANXB18 could promote metabolic activity at 5, 10, 20, and 40 μg/mL while no significant impact on metabolic activity was observed at 80 μg/mL. Co-culture RAW264.7 cells with r<em>Eg</em>ANXB18 resulted in significantly upregulation of the transcript levels of polarization markers iNOS and Arg1. Moreover, r<em>Eg</em>ANXB18 significantly upregulated the transcript levels of IL-1β, IL-6, TNFα, and IL-10, while dose-effect relationship was observed in IL-1β, IL-6, and IL-10. Our results indicated that <em>Eg</em>ANXB18 showed the potential to regulate immune response of macrophages by shifting the cell polarization and cytokine profile, thereby promoting the parasitism of CE.</p></div>","PeriodicalId":12117,"journal":{"name":"Experimental parasitology","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140021188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tegumental surface change in Paramphistomum epiclitum caused by Bombax ceiba flowers and black pepper seed extract","authors":"Kulvadee Dolsophon ,&nbsp;Sirapat Nak-on ,&nbsp;Thapana Chontananarth","doi":"10.1016/j.exppara.2024.108724","DOIUrl":"10.1016/j.exppara.2024.108724","url":null,"abstract":"<div><p>Rumen flukes, parasites of the superfamily Paramphistomoidea, are found in cattle rumen. Heavy infections can cause symptoms such as diarrhea, weight loss, and poor body condition, resulting in a decrease in milk and meat production. This study compares the tegumental surface change of <em>Paramphistomum epiclitum</em> as a response to ethanolic extracts of <em>Bombax ceiba</em> flowers and black pepper seeds. Adult flukes were subjected to various concentrations of crude extracts, including 12.5, 25, 50, 100, and 200 μg/mL for 12, 18, and 24 h incubation. Scanning electron microscopy (SEM) exhibited that the ethanolic extracts of both <em>Bombax ceiba</em> flowers and black pepper seeds caused tegumental surface changes in adult <em>P. epiclitum</em>. Based on the results, <em>Bombax ceiba</em> flower extract has anthelmintic activity, compared with black pepper seed extract, towards adult <em>P. epiclitum</em> due to the deformation of the tegument at lower concentrations than black pepper extract.</p></div>","PeriodicalId":12117,"journal":{"name":"Experimental parasitology","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140021190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Histopathological and morphological characterization of Cysticercus fasciolaris isolated from domestic and wild rodents in Morocco","authors":"Mohamed Echchakery ,&nbsp;Ali Boumezzough ,&nbsp;Mohamed Hafidi ,&nbsp;Samia Boussaa","doi":"10.1016/j.exppara.2024.108726","DOIUrl":"10.1016/j.exppara.2024.108726","url":null,"abstract":"<div><p><em>Cysticercus fasciolaris</em> is a parasitic helminth that usually infects feline and canine mammal hosts. The intermediate hosts (rodents, occasionally lagomorphs, and humans) get infected by the consumption of feed or water contaminated with eggs. Rodents are vectors of disease and reservoirs of various zoonotic parasites. The current survey was aimed at determining endoparasitic helminth infections in rodents in central Morocco. Sampled rodents after specific identification were sacrificed and examined to identify parasitic helminths following ethical guidelines. Parasites were identified using morphological characteristics. A total of 197 specimens of rodents were collected and examined in this study. Ten rodent species were identified morphologically as <em>Rattus rattus, R. norvegicus, Apodemus sylvaticus, Mus musculus, M. spretus, Mastomys erythroleucus, Meriones shawi, M. libycus, Gerbillus campestris,</em> and <em>Lemniscomys barbarus.</em> The parasitological results showed that metacestode of tapeworms was found encysted in the liver, the larval stage of <em>Taenia taeniaeformis</em> develops large multinodular fibrosarcomas which envelope the tapeworm cysts in the liver of the <em>R. rattus</em> and <em>R. norvegicus.</em> Based on morphological data, the metacestode was identified as <em>C. fasciolaris</em> in 23 (23/80) <em>R. rattus</em> 2 (2/8) and <em>R. norvegicus</em> with a prevalence of 11.7 % and 1.0 %, respectively. Rodents are major vectors of human and domestic animal diseases worldwide, and therefore, important parasitic zoonotic agents (<em>C. fasciolaris</em>), which are transmitted by black rats (<em>R. rattus</em>) and brown rats (<em>R. norvegicus</em>), must be considered to prevent the infectivity of humans, domestic animals, and livestock such as cattle, sheep, and rabbits.</p></div>","PeriodicalId":12117,"journal":{"name":"Experimental parasitology","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2024-02-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140012519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stimulation of microvesicle secretion in Trichomonas vaginalis","authors":"Júlio César Santana de Andrade ,&nbsp;Marlene Benchimol ,&nbsp;Wanderley de Souza","doi":"10.1016/j.exppara.2024.108722","DOIUrl":"10.1016/j.exppara.2024.108722","url":null,"abstract":"<div><p><em>Trichomonas vaginalis</em> is an extracellular flagellate protozoan and the etiological agent of human trichomoniasis, a sexually transmitted infection (STI) with a high incidence. Several reports have shown that this protozoan releases microvesicles into the culture medium, which show high potential in modulating cell-to-cell communication and the host response to infections. However, the biogenesis of these vesicles has not been analyzed in detail. In the present study, high-resolution ion scanning microscopy (SEM) and transmission electron microscopy (TEM) were used to analyze the surface of control cells and cells incubated in the presence of Ca²⁺ alone or with A 23187 calcium ionophore. Two different strains of <em>T. vaginalis</em> were analyzed. Most control cells displayed relatively smooth surfaces, whereas cells incubated with Ca²⁺ had many surface projections of variable shape and size (from 40 nm to around 1 μm). Quantitative analyses were performed directly in the scanning electron microscope and showed a significant increase in the number of cells with surface projections after incubation in the presence of calcium. TEM showed that treated cells presented several cytoplasmic multivesicular structures, suggesting membrane fusion and exosomes in the extracellular medium. The amount and size of the released vesicles were quantitatively analyzed using light scattering and TEM on negatively stained samples.</p><p>The observations show that incubation of both parasite strains in the presence of Ca²⁺ significantly increased the release of microvesicles into the extracellular medium in a time-dependent process. Sequential incubation in the presence of Ca²⁺ and the calcium ionophore A23187 increases the presence of vesicles on the parasite surface only at a short incubation time (5 min). Transmission electron microscopy showed that at least part of the vesicles are originated from cytoplasmic multivesicular structures. This information contributes to a better understanding of the biogenesis of extracellular vesicles secreted by <em>T. vaginalis</em>.</p></div>","PeriodicalId":12117,"journal":{"name":"Experimental parasitology","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2024-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139917470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Manganese- and zinc-coordinated interaction of Schistosoma japonicum glutathione S-transferase with neurotransmitter transporters GlyT1 and GAT3 in vitro","authors":"Martina Baliova,&nbsp;Frantisek Jursky","doi":"10.1016/j.exppara.2024.108721","DOIUrl":"10.1016/j.exppara.2024.108721","url":null,"abstract":"<div><p>Glutathione S-transferases (GSTs) are a family of multifunctional isoenzymes involved in the neutralization of toxic compounds, drug resistance and several other cellular functions. The glutathione S-transferase enzyme of <em>Schistosoma japonicum</em> (<em>Sj</em>GST-26) plays a role in human schistosomiasis and is also a frequently used fusion partner in mammalian and bacterial expression and pull-down systems. GSTs seem not to be naturally associated with metal ions<em>.</em> Exceptionally, <em>in vitro</em>, metal binding sites have been previously described in some schistosome GSTs; however, their possible physiological role is unclear. Molecules of several neurotransmitter transporters also contain a regulatory zinc binding site, which affects their transport cycle. Here we show that among several metals, manganese and zinc are able to induce a specific protein interaction of <em>Sj</em>GST-26 with the glycine transporter GlyT1 and the GABA transporter GAT3 <em>in vitro</em>. The results suggest that metal-binding sites on <em>Sj</em>GST-26 and neurotransmitter transporters might function in metal-coordinated interactions with other metalloproteins. Our results additionally indicate that the presence of metal ions in <em>Sj</em>GST-26-based GST protein pull-down assays may lead to a false-positive interaction if the potential interacting target is the metalloprotein.</p></div>","PeriodicalId":12117,"journal":{"name":"Experimental parasitology","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2024-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139899616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessing the effectiveness of fenbendazole supramolecular complexes against schistosomiasis, monieziosis and parabronemosis in sheep","authors":"Firuza Akramova ,&nbsp;Ulugbek Shakarbaev ,&nbsp;Dilbar Paluaniyazova ,&nbsp;Abror Akbarov ,&nbsp;Salavat Khalikov ,&nbsp;Alisher Safarov ,&nbsp;Djalaliddin Azimov ,&nbsp;Mourad Ben Said","doi":"10.1016/j.exppara.2024.108714","DOIUrl":"10.1016/j.exppara.2024.108714","url":null,"abstract":"<div><p>Helminth infections pose a significant economic threat to livestock production, causing productivity declines and, in severe cases, mortality. Conventional anthelmintics, exemplified by fenbendazole, face challenges related to low solubility and the necessity for high doses. This study explores the potential of supramolecular complexes, created through mechanochemical modifications, to address these limitations. The study focuses on two key anthelmintics, praziquantel (PZQ) and fenbendazole (FBZ), employing mechanochemical techniques to enhance their solubility and efficacy. Solid dispersions (SD) of PZQ with polymers and dioctyl sulfosuccine sodium (DSS) and fenbendazole with licorice extract (ES) and DSS were prepared. The helminthicidal activity of these complexes was assessed through helminthological dissections of sheep infected with <em>Schistosoma turkestanicum</em>, moniesiasis, and parabronemosis. In the assessment of supramolecular complex of FBZ (SMCF) at doses ranging from 1.0 to 3.0 mg/kg for the active substance (AS), optimal efficacy was observed with the fenbendazole formulation containing arabinogalactan and polyvinylpyrrolidone at a 3.0 mg/kg dosage. At this concentration, the formulation demonstrated a remarkable 100% efficacy in treating spontaneous monieziosis in sheep, caused by <em>Moniezia expansa</em> (Rudolphi, 1810) and <em>M. benedenii</em> (Moniez, 1879). Furthermore, the SMCF, administered at doses of 1.0, 2.0, and 3.0 mg/kg, exhibited efficacy rates of 42.8%, 85.7%, and 100%, respectively, against the causative agent of parabronemosis (<em>Parabronema skrjabini</em> Rassowska, 1924). Mechanochemical modifications, yielding supramolecular complexes of PZQ and FBZ, present a breakthrough in anthelmintic development. These complexes address solubility issues and significantly reduce required doses, offering a practical solution for combating helminth infections in livestock. The study underscores the potential of supramolecular formulations for revolutionizing helminthiasis management, thereby enhancing the overall health and productivity of livestock.</p></div>","PeriodicalId":12117,"journal":{"name":"Experimental parasitology","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2024-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139898000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The larvicidal effect of the supernatant of Lactobacillus acidophilus ATCC 4356 on Toxocara canis","authors":"Carolina Netto de Oliveira da Cunha ,&nbsp;Stéfani Rodeghiero Collares ,&nbsp;Débora Carvalho Rodrigues ,&nbsp;Débora Liliane Walcher ,&nbsp;Micaele Quintana de Moura ,&nbsp;Lourdes Helena Rodrigues Martins ,&nbsp;Gabriel Baracy Klafke ,&nbsp;Jean Lucas de Oliveira Arias ,&nbsp;Rodolfo Carapelli ,&nbsp;João Batista do Santos Espinelli Junior ,&nbsp;Carlos James Scaini ,&nbsp;Luciana Farias da Costa de Avila","doi":"10.1016/j.exppara.2024.108720","DOIUrl":"10.1016/j.exppara.2024.108720","url":null,"abstract":"<div><p>Human toxocariasis is a parasitic anthropozoonosis that is difficult to treat and control. A previous study carried out with <em>Lactobacillus acidophilus</em> ATCC 4356 revealed that the cell free supernatant (CFS) of this probiotic killed 100% of <em>Toxocara canis</em> larvae <em>in vitro</em>. The present study aimed to investigate the characteristics of the CFS of <em>L. acidophilus</em> ATCC 4356, which may be involved in its larvicidal effects on <em>T. canis</em>. <em>L. acidophilus</em> ATCC 4356 was cultured, and lactic and acetic acids present in the CFS were quantified by high performance liquid chromatography (HPLC). The levels of pH and H₂O₂ were also analyzed. To assess the larvicidal effect of the CFS, this was tested pure and diluted (1:2 to 1:128) on <em>T. canis</em> larvae. High concentrations of lactic and acetic acids were detected in the CFS. The acidity of the pure CFS was observed at pH 3.8, remaining acidic at dilutions of 1:2 to 1:16. Regarding the <em>in vitro</em> larvicidal effect, 100% death of <em>T. canis</em> larvae was observed using the pure CFS and 1:2 dilution. Based on these results, it can be inferred that the presence of higher concentrations of organic acids and low pH of the medium contributed to the larvicidal activity of the CFS of <em>L. acidophilus</em> ATCC 4356. In addition, the maintenance of the larvicidal effect, even after dilution, suggests a greater chance of the larvicidal effect of this CFS against <em>T. canis in vivo</em>.</p></div>","PeriodicalId":12117,"journal":{"name":"Experimental parasitology","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2024-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139898001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A potential role of protein extractions from Metagonimus yokogawai in amelionating inflammation in patients with ankylosing spondylitis","authors":"Eun Jeong Won ,&nbsp;Yu Jeong Lee ,&nbsp;Moon-Ju Kim ,&nbsp;Hae-In Lee ,&nbsp;Hyun Hee Jang ,&nbsp;Seong Hoon Kim ,&nbsp;Hee Min Yoo ,&nbsp;Namki Cho ,&nbsp;Seung Cheol Shim ,&nbsp;Tae-Jong Kim","doi":"10.1016/j.exppara.2024.108718","DOIUrl":"10.1016/j.exppara.2024.108718","url":null,"abstract":"<div><p>Helminth infections and their components has been recognized to have a positive impact on the immune system. This study aimed to investigate the potential of <em>Metagonimus yokogawai</em>-derived proteins (MYp) to provide protection against ankylosing spondylitis (AS) through modulation of immune responses. The cytotoxicity of MYp at various doses was first assessed using MTS and flow cytometry. Peripheral blood mononuclear cells (PBMCs) were collected from AS patients, and the production of inflammatory cytokines was analyzed through flow cytometry. In the experiments with SKG mice, MYp or vehicle was administered and inflammation was evaluated through immunohistochemistry and enzyme-linked immunosorbent assay. The results showed that MYp did not decrease cell viability of PBMCs even after 48 h. Additionally, the frequencies of IFN-γ and IL-17A producing cells were significantly reduced after MYp treatment in the PBMC cultures. Furthermore, MYp treatment significantly suppressed arthritis and enthesitis in the SKG mouse model. The results suggest the first evidence that MYp can effectively alleviate clinical symptoms and restore cytokine balance in patients with AS.</p></div>","PeriodicalId":12117,"journal":{"name":"Experimental parasitology","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2024-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139899561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}