尼日利亚一些植物中的化合物的抗锥虫和抗利什曼病活性。

IF 1.4 4区医学 Q3 PARASITOLOGY

Experimental parasitology Pub Date : 2024-10-05 DOI:10.1016/j.exppara.2024.108844

Samya Alenezi , Ngozichukwuka P. Igoli , Alexander I. Gray , Enimie E. Oaikhena , Ibrahim A. Alfayez , Harry P. de Koning , John O. Igoli

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引用次数: 0

摘要

对从三种尼日利亚植物中提取的 10 种化合物、6 种提取物和 5 种馏分进行了体外抗锥虫和抗利什曼活性测定。每种植物都用正己烷、乙酸乙酯和甲醇进行提取。根据核磁共振化学位移以及与文献报告的比较，对分离出的化合物进行了表征和鉴定。对粗提取物、馏分和分离出的化合物进行了针对动植体寄生虫的测试：布氏锥虫 Lister 427WT 的血流形式和衍生的耐多药克隆 B48，以及墨西哥利什曼原虫 cas9/T7 和衍生的克隆 cas9ΔNT1。使用硅胶对提取物进行柱层析，得到了 10 种化合物，分别是来自 Siphonochilus aetiopicus 的 curzerenone、epi-curzerenone、chloranthene F、isofuranodienone、8(17)-12E-labdadiene-15, 16-dial 和 15-hydroxy-8(17),12E-labdadiene-16-al ，来自 Calliandra portoricensis 的 lupeol、芳樟酸和 spinasterone，以及来自 Abrus precatorius 的 abruquinone B。检测结果表明，Siphonochilus aetiopicus 和 Calliandra portoricensis 的粗提取物、馏分和化合物对布氏锥虫具有中等程度的活性，但对墨西哥利什曼原虫的活性较低。Abrus precatorius 粗萃取物、馏分和分离出的化合物对测试的两种内胚层寄生虫只表现出微弱的杀锥虫和杀利什曼活性。这些发现为 Siphonochilus aetiopicus 和 Calliandra portoricensis 在与寄生虫病有关的传统医学中的应用提供了证据。

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Antitrypanosomal and antileishmanial activity of compounds from some Nigerian plants

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Antitrypanosomal and antileishmanial activity of compounds from some Nigerian plants

Ten compounds, six extracts and five fractions obtained from three Nigerian plants were assayed for their in vitro antitrypanosomal and antileishmanial activities. Each plant was extacted with hexane, ethyl acetate, and methanol. Isolated compounds were characterized and identified based on their NMR chemical shifts and comparison to literature reports. The crude extracts, fractions and isolated compounds were tested against the kinetoplastid parasites: bloodstream forms of Trypanosoma brucei Lister 427WT and the derived multi-drug resistant clone B48, and promastigote forms of Leishmania mexicana cas9/T7 and the derived clone cas9ΔNT1. Column chromatography of the extracts using silica gel yielded ten compounds identified as curzerenone, epi-curzerenone, chloranthene F, isofuranodienone, 8(17)-12E-labdadiene-15, 16-dial and 15-hydroxy-8(17),12E-labdadiene-16-al from Siphonochilus aetiopicus, lupeol, linalolic acid and spinasterone from Calliandra portoricensis, and abruquinone B from Abrus precatorius. The assay results showed that the Siphonochilus aetiopicus and Calliandra portoricensis crude extracts, fractions and compounds displayed moderate activity against the Trypanosoma brucei but showed less activity against Leishmania mexicana. Abrus precatorius crude extract, fraction, and isolated compound exhibited only weak trypanocidal and leishmanicidal activities against both kinetoplastid parasites tested. These findings have provided evidence for the use of Siphonochilus aetiopicus and Calliandra portoricensis in traditional medicine relating to parasitic diseases.

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来源期刊

Experimental parasitology 医学-寄生虫学

CiteScore

3.10

自引率

4.80%

发文量

160

审稿时长

3 months

期刊介绍： Experimental Parasitology emphasizes modern approaches to parasitology, including molecular biology and immunology. The journal features original research papers on the physiological, metabolic, immunologic, biochemical, nutritional, and chemotherapeutic aspects of parasites and host-parasite relationships.